2007 International Symposium on Micro-NanoMechatronics and Human Science最新文献_第4页

Biomechanical Role of Intracellular Structures in Smooth Muscle Cells Estimated by Traction Force Measurements 通过牵引力测量估计平滑肌细胞内结构的生物力学作用

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420888

T. Ohash, S. Nakamura, N. Sakamoto, M. Sato

{"title":"Biomechanical Role of Intracellular Structures in Smooth Muscle Cells Estimated by Traction Force Measurements","authors":"T. Ohash, S. Nakamura, N. Sakamoto, M. Sato","doi":"10.1109/MHS.2007.4420888","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420888","url":null,"abstract":"This study addresses a technique to explore mechanical role of intracellular structures by using a triton cytoskeleton model (TC model) for traction force measurements. Inhibition of signaling pathways of myosin light chain phosphorylation, possibly being induced by disruption of mictotubules, is also performed. Traction forces for the TC model significantly decreased compared to control. In contrast, traction forces significantly increased from 10.3 plusmn 2.5 nN to 13.3 plusmn 3.7 nN after treatment of nocodazole, which is well consistent with previous studies. From these results, not only cytoskeletal structures but also other cellular components such as cytoplasm should be involved in cell mechanics. Separate fluorescence studies showed that microtubules disruption induced myosin light chain phosphorylation. Exposure to Y27632 showed that traction forces decreased by 80% compared to control within 15 min and the following treatment with nocodazole showed only 40% recovery from the priori decreased forces. This result indicates that microtubules disassembly may modulate the actomyosin mechanism leading to the increase in traction forces, mainly through the ROCK pathway. It can be concluded that contribution of microtubules should include not only a force balance but also a modulator of the actomyosin-based contractile system.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130430707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of Patterned Cell Co-Cultures inside Tubular Structure Using Electrochemical Biolithography and Electrostatic Assembly 利用电化学生物光刻和静电组装技术在管状结构中产生图案细胞共培养

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420850

H. Kaji, S. Sekine, T. Abe, M. Nishizawa

{"title":"Generation of Patterned Cell Co-Cultures inside Tubular Structure Using Electrochemical Biolithography and Electrostatic Assembly","authors":"H. Kaji, S. Sekine, T. Abe, M. Nishizawa","doi":"10.1109/MHS.2007.4420850","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420850","url":null,"abstract":"We report a method for producing patterned cell co-cultures inside silicone tubing. A platinum needle microelectrode was inserted through the wall of the tubing and an oxidizing agent electrochemically generated at the inserted electrode. This agent caused local detachment of the anti-biofouling heparin layer from the inner surface of the tubing. The cell-adhesive protein fibronectin selectively adsorbed onto the newly exposed surface, making it possible to initiate a localized cell culture. The electrode could be readily set in place without breaking the tubular structure and, importantly, almost no culture solution leaked from the electrode insertion site after the electrode was removed. Ionic adsorption of poly-L-lysine at the tubular region retaining a heparin coating was used to switch the heparin surface from cell-repellent to cell-adhesive, thereby facilitating the adhesion of a second cell type. The combination of the electrode-based technique with electrostatic deposition enabled the formation of patterned co-cultures within the semi-closed tubular structure. The controlled co-cultures inside the elastic tubing should be of value for cell-cell interaction studies following application of chemical or mechanical stimuli and for tissue engineering-based bioreactors.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130680135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

4-Legged Mechanism of Realizing Dynamic Running. -Movement Performance Assessment of Locomotion Pattern by Prototype I- 实现动态跑步的四足机构。-原型机移动模式的运动性能评估

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420925

K. Morita, H. Ishihara

引用次数: 1

Development of the Maskless Exposure Device equipped with a LCD-Projector for Fabrication of Micropatterned Surfaces and Microfluidic Channels 用于制造微图案表面和微流控通道的lcd -投影仪无掩模曝光装置的研制

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420846

K. Itoga, J. Kobayashi, Y. Tsuda, M. Yamato, A. Kikuchi, T. Okano

引用次数: 4

Micro-Nano Two-Fingered Hybrid Manipulator Hand 微纳双指混合操纵手

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420822

A. Ramadan, K. Inoue, T. Arai, T. Takubo, I. Hatta

引用次数: 5

Micron Scale Measurement of a Crack Origin in a Metal Brittle Fracture 金属脆性断裂中裂纹起源的微米尺度测量

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420861

Y. Yamaguchi, Y. Kato, K. Ogata

引用次数: 2

Design and Fabrication of Tube typed Muscle Powered Bioactuator Stimulated by Circumferential Stretching System 环形拉伸系统刺激管型肌肉动力生物驱动器的设计与制造

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420891

S. Suzuki, K. Morishima

引用次数: 0

Bio-Inspired MEMS Devices for Electrical Cell Separation and Mechanical Cell Characterization 用于电细胞分离和机械细胞表征的仿生MEMS器件

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420909

Young‐Ho Cho

{"title":"Bio-Inspired MEMS Devices for Electrical Cell Separation and Mechanical Cell Characterization","authors":"Young‐Ho Cho","doi":"10.1109/MHS.2007.4420909","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420909","url":null,"abstract":"We present a couple of bio-inspired microdevices for electrical cell separation and mechanical cell characterization, respectively. The electrical cell separator, inspired from ion-selective permeation mechanism in bio-membrane, performs size-dependent cell sorting functions using electrical pillar array. The mechanical cell characterization device, inspired from deformability-selective erythrocyte destruction mechanism in spleen, performs size-independent cell deformability monitoring functions using mechanical filter array. The structures and principles of the bio-inspired microdevices are presented and compared with those of the biological counter parts. The unique features and performance characteristics of the bio-inspired microdevices are analyzed and verified by experimental study. The cell separator illustrates an electrical method for size-dependent cell sorting insensitive to cell property, while the cell deformability sensor demonstrates a mechanical method for cell deformability monitoring insensitive to cell size. Additional advantages of the bio-inspired microdevices include simple structures and stable performance for applications to integrated biomedical systems.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125395560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Advanced Simulation for Shape-prediction of 3-D PTFE Microstructures Fabricated by Using Synchrotron Radiation 同步辐射制备三维聚四氟乙烯微结构形状预测的高级模拟

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420855

M. Horade, M. Tsudo, S. Kajita, S. Sugiyama

引用次数: 0

Cell migration assay using multiple laminar flows in PDMS microchannel PDMS微通道中多层流细胞迁移实验

2007 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2007-11-01 DOI: 10.1109/MHS.2007.4420889

Fuqiang Nie, J. Kobayashi, M. Yamada, M. Yamato, A. Kikuchi, T. Okano

{"title":"Cell migration assay using multiple laminar flows in PDMS microchannel","authors":"Fuqiang Nie, J. Kobayashi, M. Yamada, M. Yamato, A. Kikuchi, T. Okano","doi":"10.1109/MHS.2007.4420889","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420889","url":null,"abstract":"Cell migration is a cellular process that plays a critical role in health and disease, including embryogenesis, wound healing, immune response, and tissue development. In order to overcome some shortcomings derived from traditional physical scrape-based cell migration assay, multiple laminar flows have been used to partially treat a confluent cell sheet formed in microchannels, which can simply pattern physiological wound edge. In the present study, rapid prototyping of poly(dimethylsiloxane) (PDMS) microchannels for assays was preformed utilizing instead a simple photopolymerization procedure adapted for a conventional liquid crystal display projector (LCDP) without any need for expensive photomasks. PDMS microchannels having three inlets (300 mum in width) that converged into a single main channel (900 mum in width) were fabricated with the LCDP-modified device. In conclusion, wound edges of confluent cell monolayers for cell migration assay were prepared utilizing trypsin laminar flow to contact only a portion of cell monolayers, within microfluidic channels. These results demonstrate that cell migration assays could be performed even in a miniaturized platform. The utilization of miniaturized microchannels can minimize the amount of expensive reagents required for cell migration assay and allows for possible scaling-up in future applications.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127778186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3