{"title":"Antireflection Micro Patterning using EB-Lithography","authors":"Y. Kathuria, S. Sugiyama","doi":"10.1109/MHS.2007.4420875","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420875","url":null,"abstract":"This paper describes the fabrication of near infra-red (NIR) antireflective micro structures on a phosphorus doped Si-wafer by direct write EB- lithography followed by RIE-etching. The Fourier transform infra-red (FTIR) measurement done from the structured surface clearly shows a reflection dip at 1.42 mum and 2.5 mum respectively. It is also found that a change in pattern size, shape and its periodicity results a shift in reflection dip accordingly. This dip in reflection spectra is caused by the surface plasmon excitation due to interaction between the incoming photon and surface plasmon resonance. Its application in the frequency selective surfaces (FSS) such as antireflection surface for effective solar radiation absorption can be realized.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126896968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Enzyme-Responsive Micelle-vesicle Transition: A New Method for the Reconstitution of Trans-membrane Protein to Liposome","authors":"K. Akiyoshi, N. Morimoto, A. Murota","doi":"10.1109/MHS.2007.4420832","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420832","url":null,"abstract":"New enzyme-responsive micellar systems in which an enzymatic reaction controls the amphiphilicity of the surfactants were reported. The surfactants in our study consist of a short alkyl chain and a maltooligomer as a primer which can be synthesized enzymatically. In the presence of phosphorylase and alpha-D-glucose-1-phosphate (G1P), the elongation reaction of the saccharide chain proceeds from the non-reducing 4-OH terminus of the glucan chain. Amylose-primer surfactants (C12MP) where an alkyl group (C12) is linked to the reduced terminus of maltopentaose form micelles in water, which are dissociated upon the enzymatic elongation reaction of the sugar moiety. The micelle-to-vesicle transition of mixed lipid/ primer systems can be controlled by using this property. This new type of transition system can be used in the reconstitution of transmembrane proteins to liposome.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131088437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Reliable Technique for Personal Identification or Verification","authors":"Hninn Thiri Thein, M. Sein, Saw Nay La Aung","doi":"10.1109/MHS.2007.4420864","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420864","url":null,"abstract":"Biometric characteristics are not unchangeable. Most biometric characteristics remain constant after they are fully developed. The hopes on biometric systems are in many respects justified and their use frequently gives good results. Fingerprint based biometric systems offer positive identification with a very high degree of confidence and compact solid state fingerprint sensors can be embedded in various systems. Fingerprinting does not require a laboratory for analysis, and fingerprints remain relatively constant over time, with the exception of injury. Each person has ten fingers, ten unique tokens tied to his or her identity. In this thesis, our objective is to design a fingerprint-based biometric system which is capable of achieving a fully automatic \"personal identification\" with a high level of confidence based on fuzzy sets in real time. Primarily the ends, branches of ridges and valleys of fingerprint are scanned. These points are unique for every person and are used to match fingerprints by their location. The following topics are covered: introduction, image requirements in acquisition and specifications, image noise reduction and enhancement, image processing methods, matching procedures and the conclusion.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123714692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sangho Kim, K. Sekiyama, T. Fukuda, K. Tanaka, K. Itoigawa
{"title":"Development of Dynamically Re-formable Input Device in Tactile and Visual Interaction","authors":"Sangho Kim, K. Sekiyama, T. Fukuda, K. Tanaka, K. Itoigawa","doi":"10.1109/MHS.2007.4420914","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420914","url":null,"abstract":"This paper describes a flexible tactile input device to operate vehicular telematics systems such as car navigation systems in quick, easy and convenient manner. The two key features of the device are: It provides visual guidance and tactual keys which can be felt. While users may press a button shown on a flat monitor under the touch screen in a conventional touch monitor, using our input device, they press a button that is tactually felt as well as visually shown. Next the tactual keys are reconfigured, being synchronized to the screen image. In a conventional touch screen configuration, surface profiles are usually fixed ones. The surface profiles of our input device are real-time reconfigured according to the screen menu. To confirm the effectiveness of the dynamically re-formable input device, experiments to compare a touch screen with our device reaction time were performed.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"153 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127327632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Mori, H. Oana, H. Atomi, T. Imanaka, M. Washizu
{"title":"Visualization of an oriC region on an isolated single whole-genome DNA with triplex forming PNA probe using fluorescence microscopy","authors":"Y. Mori, H. Oana, H. Atomi, T. Imanaka, M. Washizu","doi":"10.1109/MHS.2007.4420830","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420830","url":null,"abstract":"This paper presents a methodology for analyzing intact Mb-size DNA which enables to obtain spatial information on the position of the specific sequence in single molecular level. We utilized the complex of Qdot-streptavidin conjugate and biotinylated PNA as a triplex forming fluorescent probe and tried to visualize a region of the origin site of chromosomal replication (oriC) on an intact genomic DNA of a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1. To improve the spatial resolution along the DNA, the DNA was dragged and elongated in the solution by the optical tweezers. PNA binding to homopurine sequence in oriC region of KOD1 was confirmed by the gel electrophoresis mobility shift assay and fluorescence optical mapping in single molecular level was demonstrated. However, we have not yet detected the oriC region specifically due to the difficulty of the complete elimination of the non-specific binding between Qdot-streptavidin conjugate and DNA.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"99 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126976616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Micropatterning with different cell types by dielectrophoretic manipulation","authors":"T. Yasukawa, Masato Suzuki, H. Shiku, T. Matsue","doi":"10.1109/MHS.2007.4420848","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420848","url":null,"abstract":"In this paper, a versatile, rapid and reproducible method for patterning different cell types based on negative dielectrophoresis (n-DEP), without any special pretreatment of a culture slide, has been described. An interdigitated array (IDA) electrode with four independent microelectrode subunits was fabricated with indium-tin-oxide (ITO) and used as a template to form cellular micropatterns. A suspension of C2C12 cells was introduced into the device between the upper slide and the bottom IDA. In the present system, the n-DEP force is induced by applying an ac voltage (typically 12 Vpp, 1 MHz) to direct cells toward a weaker region of electric field strength. The cells aligned above one of the bands of IDA within 1 min since the aligned areas on the slide were regions with the lower electric field. The application of an ac voltage for 5 min allows the cells to adsorb onto the cell culture slide. After disassembling the device and removing excess cells, the culture slide was assembled again with the IDA electrode, and was rotated 90deg relative to the previous setup. The second cell type was patterned in lines using the same method as with the first set of cells, forming a grid pattern on the slide.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117349285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ohka, Y. Sawamoto, S. Matsukawa, T. Miyaoka, Y. Mitsuya
{"title":"Parallel Type Two-axial Actuator Controlled by a Multi-layered Neural Network","authors":"M. Ohka, Y. Sawamoto, S. Matsukawa, T. Miyaoka, Y. Mitsuya","doi":"10.1109/MHS.2007.4420892","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420892","url":null,"abstract":"We experimentally design a parallel typed two-axial micro actuator, which is utilized for the key part of the tactile display. The parallel typed two-axial actuator was composed of two bimorph piezoelectric elements and two small links connected by three joints. We formulated kinematics for the parallel typed two-axial actuator because the endpoint is controlled in the two-dimensional coordinate. Since relationship between applied voltage and displacement cause by the voltage shows a hysteresis loop in the bimorph piezoelectric element used as components of the two-axial actuator, we produce a control system for the two-axial actuator based on a multi-layered artificial neural network to compensate the hysteresis. The neural network is comprised of 4 neurons in the input layer, 10 neurons in the hidden layer and ones neuron in the output layer. The output neuron emits time derivative of voltage; two bits signal expressing increment or decrement condition is generated by two input neurons; one of the other two input neurons and the other calculate current values of voltage and displacement, respectively. The neural network is featured with a feedback loop including an integral element to reduce number of neurons. In the learning process, the network learns the hysteresis including a minor loop. In the verification test, the endpoint of the two-axial actuator traces the desired circular trajectory in the two-dimensional coordinate system.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131542318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of Morphological Properties of Perfluoropolyether Films on Magnetic Disks Using Molecular Dynamics Simulation","authors":"Y. Tagaya, Y. Mitsuya, Hedong Zhang, K. Fukuzawa","doi":"10.1109/MHS.2007.4420919","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420919","url":null,"abstract":"Coarse-grained molecular dynamics (MD) simulations were applied to reproduce the surface morphology of lubricant films composed of perfluoropolyether (PFPE) Zdol and tri-functional PFPE, which has a functional group at the middle of a molecule as well as functional endgroups at the head and tail of the molecule. The molecular-mesh scanning method was introduced to detect the location of film surface. The effects of the number of polar groups and polymer length on a flat solid surface were studied as lubricant films with periodic boundary conditions. We clarified that the film surface of the tri-functional PFPE lubricant was smoother than Zdol 2000 when compared at the same film thickness condition.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125292121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Display of Recombinant Membrane Receptors on Giant Liposomes: Attempt to Construct a Cell Model with Integrated Membrane Protein Systems","authors":"K. Tsumoto, K. Kamiya, T. Yoshimura","doi":"10.1109/MHS.2007.4420834","DOIUrl":"https://doi.org/10.1109/MHS.2007.4420834","url":null,"abstract":"Giant unilamellar vesicles (GUVs), or giant liposomes (GLs), are biochemical compartments enclosed with phospholipid bilayers. The size of GUVs is comparable to that of real cells (~10-100 mum). Thus, GUVs are employed to construct artificial cell models, which are reconstituted from biochemical materials to mimic real cellular functions in a simpler manner for investigation of fundamental mechanisms of living systems. However, transmembrane proteins are difficult to reconstitute on GUV bilayers. In order to obtain GUVs containing transmembrane proteins, giant proteoliposomes, here, we introduce the novel method using membrane fusion between GUVs and recombinant baculovirus (Autographa californica nucleopolyhedrovirus (AcNPV)). It is known that the AcNPV budded virus (BV) displays recombinant membrane proteins on their own envelopes. BVs have fusogenic envelope glycoproteins (gp64) sensitive to acidic pH. Combination of these two features may produce giant proteoliposomes. The fusion properties were observed using fluorescence microscopy to comprehend conditions suitable for reconstitution of proteoliposomes. The display of a 7-fold transmembrane receptor protein (G protein-coupled receptor) on GUVs was also demonstrated using fluoroimmuno microscopic observation.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"103 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125558214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Uchida, H. Oura, S. Ikeda, T. Nakano, F. Aral, M. Negoro, T. Matsuda, T. Fukuda
{"title":"Development of biodegradable scaffolds by leaching self-assembled magnetic sugar particles","authors":"T. Uchida, H. Oura, S. Ikeda, T. Nakano, F. Aral, M. Negoro, T. Matsuda, T. Fukuda","doi":"10.1109/MHS.2009.5351884","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351884","url":null,"abstract":"Technologies to develop scaffolds with controlled diameter and high porosity have great significance in tissue engineering. We have fabricated biodegradable 2D and 3D scaffolds with ordered array of pores by casting polymer on self-assembled d-fructose (sugar) microspheres. First, ferrite microparticles were encapsulated in sugar spheres to make them become magnetized. After sieving magnetic sugar particles, those diameter-controlled particles were attracted by a magnet to form a self-assembled template for polymer casting. Interspaces of self-assembled template were successfully infiltrated with polymer. After removal of sugar particles, ordered array of pores were generated on the surface of scaffolds. Elastic modulus of a sheet-like scaffold was measured to be 2.0 MPa and was reasonably lower than that of a nonporous sheet. The biocompatibility of the developed scaffold was confirmed by the viability of human umbilical vein endothelial cells.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"156 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129307742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}