{"title":"单分子观察监测光诱导DNA双链断裂:抗氧化化合物的保护作用","authors":"Yuko Yoshikawa, Etsuko Hirose, Emi Sakai, Chiharu Ikawa","doi":"10.1109/MHS.2007.4420866","DOIUrl":null,"url":null,"abstract":"We have performed a real-time observation of photo-induced breakage on individual long DNA molecules stained by a cyanine dye, YOYO, under the intense illumination in solution. It is known that YOYO has ability to photo-cleave probed DNA by generating reactive oxygen species. We analyzed the kinetics of the breakage reaction in a quantitative manner. It was found that the breakage reaction is protected by antioxidative compounds; ascorbic acid and a water-soluble flavonoid, glucosyl-rutin. To interpret the kinetic process of the double-strand breakage reaction, a two-step mechanism is proposed.","PeriodicalId":161669,"journal":{"name":"2007 International Symposium on Micro-NanoMechatronics and Human Science","volume":"43 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2007-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Photo-Induced DNA Double-Strand Breaks Monitored by Single-Molecule Observation: Protective Effect of Antioxidative Compounds\",\"authors\":\"Yuko Yoshikawa, Etsuko Hirose, Emi Sakai, Chiharu Ikawa\",\"doi\":\"10.1109/MHS.2007.4420866\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We have performed a real-time observation of photo-induced breakage on individual long DNA molecules stained by a cyanine dye, YOYO, under the intense illumination in solution. It is known that YOYO has ability to photo-cleave probed DNA by generating reactive oxygen species. We analyzed the kinetics of the breakage reaction in a quantitative manner. It was found that the breakage reaction is protected by antioxidative compounds; ascorbic acid and a water-soluble flavonoid, glucosyl-rutin. To interpret the kinetic process of the double-strand breakage reaction, a two-step mechanism is proposed.\",\"PeriodicalId\":161669,\"journal\":{\"name\":\"2007 International Symposium on Micro-NanoMechatronics and Human Science\",\"volume\":\"43 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2007 International Symposium on Micro-NanoMechatronics and Human Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/MHS.2007.4420866\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2007 International Symposium on Micro-NanoMechatronics and Human Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/MHS.2007.4420866","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Photo-Induced DNA Double-Strand Breaks Monitored by Single-Molecule Observation: Protective Effect of Antioxidative Compounds
We have performed a real-time observation of photo-induced breakage on individual long DNA molecules stained by a cyanine dye, YOYO, under the intense illumination in solution. It is known that YOYO has ability to photo-cleave probed DNA by generating reactive oxygen species. We analyzed the kinetics of the breakage reaction in a quantitative manner. It was found that the breakage reaction is protected by antioxidative compounds; ascorbic acid and a water-soluble flavonoid, glucosyl-rutin. To interpret the kinetic process of the double-strand breakage reaction, a two-step mechanism is proposed.
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