Journal of Inflammation Research最新文献

{"title":"A Nomogram Based on Circulating Inflammatory Factors for Predicting Prognosis of Newly Diagnosed Multiple Myeloma Patients.","authors":"Mowang Wang, Xiaoyan Yue, Yingying Ding, Zhen Cai, Haowen Xiao, He Huang, Jingsong He","doi":"10.2147/JIR.S495284","DOIUrl":"10.2147/JIR.S495284","url":null,"abstract":"<p><strong>Purpose: </strong>The growth and survival of multiple myeloma (MM) cells depend heavily on bone marrow microenvironment, where inflammation emerges as a significant feature and is commonly associated with unfavorable prognosis in MM. Our previous study and other published studies have shown that MM patients with higher neutrophil-to-lymphocyte ratio (NLR) or interleukin (IL)-10 (IL-10), lower lymphocyte-to-monocyte ratio (LMR) or platelet-to-lymphocyte ratio (PLR) frequently have inferior overall survival (OS) independent of current risk- stratification markers. Nevertheless, whether specific inflammation-related markers have prognostic value for MM patients remains elusive.</p><p><strong>Patients and methods: </strong>We retrospectively analyzed the clinical data of 452 newly diagnosed MM (NDMM) patients treated in our center from May 2013 to June 2022. Cox regression analysis and least absolute shrinkage and selector operation (LASSO) were performed to establish the predictive nomograms for survival outcomes in the training cohort, and the nomograms were validated by calibration curves in the validation cohort.</p><p><strong>Results: </strong>The best cutoff values of NLR, LMR, PLR, and IL-10 were 4.44, 4.0, 100, and 1.42pg/mL, respectively. We established a nomogram model after LASSO Cox and multivariate Cox regression analysis. The nomogram model exhibited acceptable discrimination, with C-index values of 0.777, 0.714, and 0.71 in the training cohort, validation cohort, and entire cohort, respectively, which was significantly higher than the C-indices of the three most extensively used staging systems for NDMM (D-S, ISS, and R-ISS). All calibration curves revealed good consistency between the predictive and actual survival outcomes. Patients were divided into high-risk and low-risk groups based on their total nomogram scores, with a threshold of 106.2, where the median OS of patients in the high-risk group was significantly shorter than that of patients in the low-risk group.</p><p><strong>Conclusion: </strong>The proposed nomogram based on circulating inflammatory factors is an inexpensive, widely available, and easily interpretable risk-stratification tool for NDMM patients.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"2077-2090"},"PeriodicalIF":4.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Prospective Comparative Study on the Clinical Diagnostic Performance of Blood Inflammatory Markers in Acute Appendicitis [Letter].","authors":"Rıfat Peksöz, Enes Ağırman, Sabri Selçuk Atamanalp","doi":"10.2147/JIR.S518021","DOIUrl":"10.2147/JIR.S518021","url":null,"abstract":"","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"2123-2124"},"PeriodicalIF":4.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829649/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and Anti-Inflammatory Potential of <i>Cymbopogon nardus</i> Ethanol Extract on <i>3T3-L1</i> Cells.","authors":"Enny Rohmawaty, Hesti Lina Wiraswati, Tamara Aliya Zahra, Shabrina Nur Amalina, Julia Ramadhanti, Aziiz Mardanarian Rosdianto, Amila Laelalugina, Gita Tiara Dewi Nasution, Yusof Kamisah","doi":"10.2147/JIR.S506189","DOIUrl":"10.2147/JIR.S506189","url":null,"abstract":"<p><strong>Purpose: </strong><i>Cymbopogon nardus</i> (L). Rendle has traditionally been recognized for its medicinal properties. Recent studies have suggested that its bioactive constituents possess antioxidant and anti-inflammatory properties. However, there is limited scientific evidence of its cellular effects. Given that the pathogenesis of many diseases involves oxidative stress and inflammation, this study aimed to evaluate the potential antioxidant and anti-inflammatory effects of the plant extracts in 3T3-L1 cells.</p><p><strong>Methods: </strong>Phytochemical screening of <i>C. nardus</i> extracts was performed to identify bioactive compounds. Antioxidant activity of the extract was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide dismutase (SOD) assays. Toxicity was evaluated using the MTT assay. Additionally, the effects of the extract on the gene expression of hypoxia-inducible factor 1α (HIF-1α) in menadione-induced 3T3-L1 cells, as well as interleukin-6 (IL-6) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-induced 3T3-L1 cells, were investigated.</p><p><strong>Results: </strong>Phytochemical screening revealed the presence of phenolics, tannins, alkaloids, and flavonoids in the ethanolic extracts. The extract demonstrated antioxidant activity, with IC₅₀ values of 178.06 ppm for DPPH and 220 ppm for SOD. It did not affect the viability of 3T3-L1 cells at concentrations of up to 500 ppm. At 100 ppm, the extract increased cell viability (p<0.05) and reduced HIF-1α expression in the menadione-treated cells (p<0.05). Additionally, it decreased the expression of IL-6 and COX-2 in LPS-induced cells (p<0.05).</p><p><strong>Conclusion: </strong>The ethanol extract of <i>C. nardus</i> demonstrated promising potential as an antioxidant and anti-inflammatory agent in 3T3-L1 cells. Further analysis is recommended to confirm the potential.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"2125-2136"},"PeriodicalIF":4.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"B Cell Activation, Differentiation, and Their Potential Molecular Mechanisms in Osteoarthritic Synovial Tissue.","authors":"Peizhi Lu, Ya Li, Shuo Yang, Haoyu Yao, Bizhi Tu, Rende Ning","doi":"10.2147/JIR.S503597","DOIUrl":"10.2147/JIR.S503597","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to characterize the activation and differentiation of B cells in the synovium of osteoarthritis (OA) and to explore the underlying molecular mechanisms.</p><p><strong>Methods: </strong>Peripheral blood and synovial samples from OA patients at different stages were collected, and flow cytometry was employed to analyze the activation and differentiation of B cells. Immunofluorescence staining of joint synovium from OA mice at different stages was conducted to assess mice joint synovium B cell activation and differentiation. Co-culture experiments of synovial fibroblasts with B cells were performed to investigate the influence of synovial cells on B cell activation and differentiation. Finally, transcriptome analysis was utilized to identify potential key molecules and pathways.</p><p><strong>Results: </strong>In OA patients, the infiltration, activation, and differentiation of B cells in synovium and peripheral blood exhibited distinct characteristics. Specifically, the proportion of activated CD86+ B cells and the differentiation marker HLA-DR+ increased with disease severity, whereas the proportion of the differentiation marker IgM decreased. The proportion of CD38+ B cells also decreased with increasing severity, although this change lacked statistical significance. Immunofluorescence staining of CD19+ and CD86+ cells in mice indicated increased expression with greater OA severity. Co-culture experiments demonstrated that OA synovial fibroblasts promoted B cell activation and differentiation, as evidenced by higher expression levels of CD86+ and HLA-DR+ in the OA group compared to controls. Additionally, the proportion of naive B cells decreased as disease severity progressed.</p><p><strong>Conclusion: </strong>Synovial fibroblasts in OA have been shown to promote the differentiation and activation of B cells, indicating that B cells play a significant role in the pathogenesis of synovium inflammation in OA.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"2137-2151"},"PeriodicalIF":4.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing the Predictive Value of SII and NLR in LAA Stroke: Addressing Unexplored Limitations and Future Directions [Response to Letter].","authors":"Zemin He, Keting Liu","doi":"10.2147/JIR.S520075","DOIUrl":"10.2147/JIR.S520075","url":null,"abstract":"","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"2031-2032"},"PeriodicalIF":4.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Analysis of Key Immune- and Inflammation-Related Genes in Idiopathic Pulmonary Fibrosis.","authors":"Yan Tan, Baojiang Qian, Qiurui Ma, Kun Xiang, Shenglan Wang","doi":"10.2147/JIR.S489210","DOIUrl":"10.2147/JIR.S489210","url":null,"abstract":"<p><strong>Background: </strong>Studies suggest that immune and inflammation processes may be involved in the development of idiopathic pulmonary fibrosis (IPF); however, their roles remain unclear. This study aims to identify key genes associated with immune response and inflammation in IPF using bioinformatics.</p><p><strong>Methods: </strong>We identified differentially expressed genes (DEGs) in the GSE93606 dataset and GSE28042 dataset, then obtained differentially expressed immune- and inflammation-related genes (DE-IFRGs) by overlapping DEGs. Two machine learning algorithms were used to further screen key genes. Genes with an area under curve (AUC) of > 0.7 in receiver operating characteristic (ROC) curves, significant expression and consistent trends across datasets were considered key genes. Based on these key genes, we carried out nomogram construction, enrichment and immune analyses, regulatory network mapping, drug prediction, and expression verification.</p><p><strong>Results: </strong>27 DE-IFRGs were identified by intersecting 256 DEGs, 1793 immune-related genes, and 1019 inflammation-related genes. Three genes (<i>RNASE3, S100A12, S100A8</i>) were obtained by crossing two machine algorithms (Boruta and LASSO),which had good diagnostic performance with AUC values. These key genes were all enriched in the same pathways, such as GOCC_azurophil_granule, IL-12 signalling and production in macrophages is the pathway with the strongest role for key genes. Six distinct immune cells, including naive CD4 T cells, T cells CD4 memory resting, T cells regulatory (Tregs), Monocytes, Macrophages M2, Neutrophils were identified. Real-time quantitative polymerase chain reaction (RT-qPCR) results were consistent with the training and validation sets, and the expression of these key genes was significantly upregulated in the IPF samples.</p><p><strong>Conclusion: </strong>This study identified three key genes (<i>RNASE3, S100A12</i> and <i>S100A8</i>) associated with immune response and inflammation in IPF, providing valuable insights into the diagnosis and treatment of IPF.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"1993-2009"},"PeriodicalIF":4.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights and Considerations for \"Higher Intraocular Levels of Inflammatory Factors Are Related to Retinal Vascular and Neurodegeneration in Myopic Retinopathy\" [Response to Letter].","authors":"Ling Zeng, Zhikuan Yang, Xiaoning Li, Heping Xu","doi":"10.2147/JIR.S516797","DOIUrl":"10.2147/JIR.S516797","url":null,"abstract":"","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"2073-2075"},"PeriodicalIF":4.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Features and Prognosis of Double-Positive Anti-MDA5 and Anti-CCP Antibodies in Dermatomyositis: A Retrospective Study.","authors":"Xiayu Xu, Longyang Zhu, Sizhao Li, Guochun Wang, Yongpeng Ge","doi":"10.2147/JIR.S503120","DOIUrl":"10.2147/JIR.S503120","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the clinical features and prognosis of anti-melanoma differentiation-related gene 5 (MDA5) antibody and anti-cyclic citrullinated peptide (CCP) antibody double-positive dermatomyositis (DM) (MDA5+/CCP+ DM).</p><p><strong>Methods: </strong>A retrospective analysis of 264 consecutive cases of MDA5+ DM hospitalized from March 2018 to March 2022, and patients with anti-CCP antibodies were screened out. Patients from MDA5+/CCP- served as the control. Propensity score matching was used to compare the clinical features, as well as the treatment and survival outcomes between the two groups.</p><p><strong>Results: </strong>A total of 18 patients (6.8%) with MDA5+/CCP+ DM were identified. Gottron's sign in 17 cases (94.4%), Heliotrope rash in 14 cases (77.8%), and skin ulcer in 5 cases (27.8%). Arthritis occurred in 10 cases (55.6%) and presented as the initial symptom in 6 cases (33.3%). Only 8 (44.4%) of the patients met the criteria for rheumatoid arthritis (RA). Interstitial lung disease (ILD) was diagnosed in 17 cases (94.4%), with non-specific interstitial pneumonia (NSIP) and organizing pneumonia (OP) combined with NSIP being the most predominant, as they presented in 6 cases (33.3%) each. Two of the ILD cases (11.1%) developed into rapidly progressive ILD (RP-ILD). Arthritis (55.6% vs 15.3%, <i>p</i> = 0.001) and malignancy (22.2% vs 0%, <i>p</i> < 0.001) were significantly more common in the MDA5+/CCP+ group compared to the MDA5+/CCP- group. The clinical manifestations of MDA5+/CCP+ DM with or without anti-Ro-52 antibody were not significantly different from those of the ILD type. During the follow-up period, there was no significant difference in survival between the two groups.</p><p><strong>Conclusion: </strong>More than half of the patients with MDA5+/CCP+ DM may present with arthritis as one of the initial symptoms. They may also have an increased risk of malignancy. For MDA5+ DM patients with positive anti-CCP antibodies, thorough screening for tumors is crucial to guide treatment and improve prognosis.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"1929-1939"},"PeriodicalIF":4.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827484/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Potential Diagnostic Biomarkers of Carotid Atherosclerosis in Obese Populations.","authors":"Xize Wu, Jiaxiang Pan, Xue Pan, Jian Kang, Jiaqi Ren, Yuxi Huang, Lihong Gong, Yue Li","doi":"10.2147/JIR.S504480","DOIUrl":"10.2147/JIR.S504480","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the potential mechanisms and biomarkers between Obesity (OB) and carotid atherosclerosis (CAS).</p><p><strong>Methods: </strong>The GSE12828, GSE125771, GSE43292, and GSE100927 datasets were combined and normalized to obtain CAS-related differentially expressed genes (DEGs), and OB-related DEGs were obtained from the GSE151839 dataset and the GeneCards database. Unsupervised cluster analysis was conducted on CAS samples based on the DEGs of CAS and OB. Subsequently, immune infiltration analysis and gene set enrichment analysis (GESA) were performed. 61 machine learning models were developed to screen for Hub genes. The Single-gene GESA focused on calcium signaling pathway-related genes (CaRGs). Finally, high-fat diet-fed C57BL/6J ApoE^-/- mice were used for in vivo validation.</p><p><strong>Results: </strong>MMP9, PLA2G7, and SPP1 as regulators of the immune infiltration microenvironment in OB patients with CAS, and stratified CAS samples into subtypes with differences in metabolic pathways based on OB classification. Enrichment analysis indicated abnormalities in immune and inflammatory responses, the calcium signaling, and lipid response in obese CAS patients. The RF+GBM model identified CD52, CLEC5A, MMP9, and SPP1 as Hub genes. 15 CaRGs were up-regulated, and 12 were down-regulated in CAS and OB. PLCB2, PRKCB, and PLCG2 were identified as key genes in the calcium signaling pathway associated with immune cell infiltration. In vivo experiments showed that MMP9, PLA2G7, CD52, SPP1, FYB, and PLCB2 mRNA levels were up-regulated in adipose, aortic tissues and serum of OB and AS model mice, CLEC5A was up-regulated in aorta and serum, and PRKCB was up-regulated in adipose and serum.</p><p><strong>Conclusion: </strong>MMP9, PLA2G7, CD52, CLEC5A, SPP1, and FYB may serve as potential diagnostic biomarkers for CAS in obese populations. PLCB2 and PRKCB are key genes in the calcium signaling pathway in OB and CAS. These findings offer new insights into clinical management and therapeutic strategies for CAS in obese individuals.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"1969-1991"},"PeriodicalIF":4.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Psoriasis: Unraveling Disease Mechanisms and Advancing Pharmacological and Nanotechnological Treatments.","authors":"Miao Miao, Jiong Yan, Yujin Sun, Jia Liu, Shun Guo","doi":"10.2147/JIR.S506103","DOIUrl":"10.2147/JIR.S506103","url":null,"abstract":"<p><p>Research into the pathogenesis of inflammatory skin diseases, including dermatitis and psoriasis, has yielded significant advancements in the last decades. The identification of age, gender, and genetic factors contributing to these complex conditions has been pivotal in developing novel pharmacological and technological treatments. This review delves into the molecular underpinnings of psoriasis, examining current therapies and promising investigational agents. We highlight the potential of nanotechnology to enhance drug delivery to affected skin areas, with microneedles emerging as a promising platform for psoriasis and other chronic inflammatory skin diseases.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"2045-2072"},"PeriodicalIF":4.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}