Journal of Inflammation Research最新文献

{"title":"Proteomic Profiling and Clinical Insights: The Role of MMP9 in Differentiating Psoriasis Vulgaris from Generalized Pustular Psoriasis.","authors":"Ting Gong, Jiawen Chen, Zhixun Xiao, Renwei Luo, Zequn Tong, Hui Ke, Zhao Liu, Cuirong Xiao, Niu Xiang, Chao Ji","doi":"10.2147/JIR.S495044","DOIUrl":"10.2147/JIR.S495044","url":null,"abstract":"<p><strong>Background: </strong>Generalized pustular psoriasis (GPP) constitutes a rare, severe inflammatory disorder that differs from psoriasis vulgaris (PV). The IL-36 pathway has been identified as a key element in GPP pathogenesis.</p><p><strong>Objective: </strong>To explore protein expression between PV and GPP, providing insights into potential mechanisms.</p><p><strong>Methods: </strong>We performed proteomic analysis of tissue specimens from patients with PV and GPP to identify differentially expressed proteins. Comparative analysis of the proteomic data was performed and proteins with significant differences were further identified using immunofluorescence and Western blot techniques. Differential proteins were also explored by evaluating the efficacy of IL-36R inhibitors before and after GPP treatment, providing potential avenues for targeted therapeutic strategies.</p><p><strong>Results: </strong>Tissue proteomic profiling showed that matrix metallopeptidase 9 (MMP9) increased significantly in the GPP as compared to PV. Immunofluorescence and Western blot analysis confirmed that MMP9 is higher expressed in GPP. And after therapy with IL-36 inhibitors showed that the level of MMP9 expression was markedly reduced.</p><p><strong>Conclusion: </strong>MMP9 may be involved with the pathogenesis of GPP.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"3795-3805"},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920628/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Clinical Predictive Model Based on SOCS3 Promoter Methylation to Predict the Prognosis of Acute-on-Chronic Hepatitis B Liver Failure.","authors":"Ji-Hui Li, Yuna Tang, Jing Wang, Xue-Fei Wei, Na Wang, Jing-Wei Wang, Hui Lyu, Xue-Mei Jiang, Hui-Hui Liu, Kai Wang","doi":"10.2147/JIR.S506050","DOIUrl":"10.2147/JIR.S506050","url":null,"abstract":"<p><strong>Purpose: </strong>The study aimed to quantitatively detect the suppressors of cytokine signaling (SOCS) 3 promoter methylation levels, investigate the relationship between SOCS3 methylation and gene expression, and construct a prognosis prediction model combined with clinical indicators for Acute-on-chronic Hepatitis B Liver Failure (ACHBLF).</p><p><strong>Methods: </strong>A total of 135 ACHBLF patients were enrolled and randomly divided into the training cohort and validation cohort. The SOCS3 mRNA and promoter methylation in peripheral blood mononuclear cells (PBMCs) of ACHBLF patients were quantitative measured. A clinical prediction model was established based on SOCS3 promoter methylation and clinical indicators. The prediction model was evaluated by the area under the receiver operating characteristic curve, the Hosmer-Lemeshow (H-L) goodness-of-fit test, and decision curve analysis.</p><p><strong>Results: </strong>In this study, compared with ACHBLF survivals, SOCS3 showed lower mRNA levels and higher methylation levels in ACHBLF non-survivals. The SOCS3 methylation rates were negatively correlated with SOCS3 mRNA levels. PT-INR, IL-6, and percentage of the methylation reference (PMR) value (SOCS3) were used to establish a clinical model for predicting ACHBLF patients' prognosis. The results of AUC, the Hosmer-Lemeshow (H-L) goodness-of-fit test and decision curve analysis (DCA) showed that the prediction model had good clinical applicability. The prediction model was visualized.</p><p><strong>Conclusion: </strong>A prognosis prediction model for ACHBLF was developed based on PMR (SOCS3), PT-INR and IL-6, which may have a good potential clinical application value.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"3741-3756"},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Evaluation of Lipocalin-2 in Sepsis-Associated Encephalopathy via Machine Learning Approaches.","authors":"Jia Hu, Ziang Chen, Jinyan Wang, Aoxue Xu, Jinkai Sun, Wenyan Xiao, Min Yang","doi":"10.2147/JIR.S504390","DOIUrl":"10.2147/JIR.S504390","url":null,"abstract":"<p><strong>Purpose: </strong>Sepsis-associated encephalopathy (SAE) critically contributes to poor prognosis in septic patients. Identifying and screening key genes responsible for SAE, as well as exploring potential targeted therapies, are vital for improving the management of sepsis and advancing precision medicine.</p><p><strong>Patients and methods: </strong>Single-cell RNA sequencing (scRNA-seq) was administrated to identify cell subpopulations related to poor prognosis in septic patients. Next, hierarchical dynamic weighted gene co-expression network analysis (hdWGCNA) was employed to identify genes associated with specific neutrophil subpopulations. Enrichment analysis revealed the biological functions of these genes. Subsequently, neuroinflammation-related genes were obtained to construct a neuroinflammation-related signature. The AddModuleScore algorithm was used to calculate neuroinflammation scores for each cell subpopulation, whereas the CellCall algorithm was used to assess the crosstalk between neutrophils and other cell subpopulations. To identify key genes accurately, four binary classification machine learning algorithms were utilized. Finally, Western blotting and behavioral tests were used to confirm the role of LCN2-related neuroinflammation in septic mice.</p><p><strong>Results: </strong>This study utilized scRNA-seq to reveal the critical role of peripheral neutrophils during sepsis, identifying these neutrophils as contributors to poor prognosis and associated with neuroinflammation. On the basis of various machine learning algorithms, we discovered that Lipocalin-2 (LCN2) may be the key gene involved in neutrophil-induced SAE. To prove these findings, we conducted in vivo experiments and an animal model. Increased LCN2 expression and cognitive dysfunction occurred in septic mice. Additionally, the levels of markers of astrocytes and microglia and inflammatory factors such as TNF-α and IL-6 were significantly increased. All these phenomena were reversed by the downregulation of LCN2.</p><p><strong>Conclusion: </strong>The upregulation of LCN2 expression on peripheral neutrophils is a critical step that triggers neuroinflammation in the central nervous system during SAE.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"3843-3858"},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unilateral Biportal Endoscopic Debridement and Drainage for Lumbar Infectious Spondylodiscitis: A Retrospective Study and Preliminary Results.","authors":"Rupeng Chu, Wei Cui, Wenjin Chen, Yin Zhuang, Guoyong Yin, Wei Peng, Shujun Zhang","doi":"10.2147/JIR.S505707","DOIUrl":"10.2147/JIR.S505707","url":null,"abstract":"<p><strong>Background: </strong>Clinical management of lumbar infectious spondylodiscitis is challenging due to its variable presentation and complex course, and its treatment remains controversial. This study aims to evaluate the clinical efficacy of unilateral biportal endoscopic (UBE) debridement and drainage for treating lumbar infectious spondylodiscitis.</p><p><strong>Methods: </strong>We retrospectively analysed sixteen patients diagnosed with lumbar infectious spondylodiscitis who underwent UBE debridement and drainage between April 2022 and July 2023. Biopsy specimens were sent to the laboratory to identify pathogens immediately after surgeries. Clinical outcomes were assessed by the visual analog scale (VAS) scores of the back, Oswestry Disability Index (ODI), the modified MacNab criteria (MNC), and regular serological tests at pre- and post-operation.</p><p><strong>Results: </strong>Fourteen patients (87.5%) experienced a significant improvement in their clinical symptoms. Their VAS and ODI scores significantly improved compared to those before the operation throughout the follow-up (p<0.05). The modified MNC at the last follow-up indicated that 87.50% of these participants were rated excellent or good. Causative bacteria were identified in 13 (81.25%) of 16 biopsy specimens. At the final follow-up, all patients' kyphotic angle changes were less than 10° without spinal instability. A 12-month follow-up CT scan revealed bony intervertebral fusion in 10 cases (62.5%). The postoperative regular serological tests were significantly improved than before surgery (p< 0.05). No recurrent infections or significant surgery-related complications were observed during postoperative follow-up.</p><p><strong>Conclusion: </strong>UBE surgery was successful in debridement, back pain relief, and bacteriologic diagnosis of lumbar infectious spondylodiscitis. This procedure could be an effective alternative for patients when conservative treatments fail.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"3695-3704"},"PeriodicalIF":4.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Nomogram Based on Tumor Response to Induction Chemotherapy and Plasma Epstein-Barr Virus DNA Level after Induction Chemotherapy to Explore Individualized Treatment of Patients with Locally Advanced Nasopharyngeal Carcinoma.","authors":"Fushuang Liu, Chengxian Ma, Meiwen Chen, Kaihua Chen, Liru Zhu, Ling Li, Xiaodong Zhu, Song Qu, Chang Yan","doi":"10.2147/JIR.S507926","DOIUrl":"10.2147/JIR.S507926","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the influence of Epstein-Barr virus (EBV) DNA levels before and after induction chemotherapy (IC), tumor response to IC, and baseline factors on overall survival (OS) in patients with locally advanced nasopharyngeal carcinoma (LA-NPC). A nomogram was subsequently constructed to explore the individualized optimal cumulative cisplatin dose (CCD) in concurrent chemoradiotherapy (CCRT).</p><p><strong>Methods: </strong>A total of 581 LA-NPC patients were included, randomly divided into training and validation cohorts in a 7:3 ratio. In the training cohort, a nomogram was subsequently established based on multivariate Cox regression analysis and then validated. Subsequently, patients were classified into different risk groups based on the nomogram, and the impact of different levels of CCD on survival outcomes was evaluated.</p><p><strong>Results: </strong>EBV DNA levels after IC, tumor response to IC, age, and LDH were independent prognostic factors of OS. Schoenfeld residual analysis indicated overall satisfaction of the proportional hazards assumption for the Cox regression model. The C-index of the nomogram was 0.758 (95% CI: 0.695-0.821) for the training cohort and 0.701 (95% CI: 0.589-0.813) for the validation cohort. Calibration curves demonstrated good correlation between the nomogram and actual survival outcomes. DCA confirmed the clinical utility enhancement of the nomogram over the TNM staging system. For OS, patients in the medium/high-risk group with a CCD > 200 mg/m² had better outcomes than those with CCD ≤ 200 mg/m², although the difference was not statistically significant (<i>p</i> = 0.097). No significant difference was observed in local relapse-free survival (LRFS), distant metastasis-free survival (DMFS), and progression-free survival (PFS) across various levels of CCD in different risk subgroups (<i>p</i> > 0.05).</p><p><strong>Conclusion: </strong>The nomogram based on EBV DNA levels after IC, tumor response, LDH, and age effectively predicts OS in LA-NPC patients, aids in risk stratification, and may guide treatment decisions.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"3677-3693"},"PeriodicalIF":4.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of PLIN3 in Prognosis and Tumor-Associated Macrophage Infiltration: A Pan-Cancer Analysis.","authors":"Shaohua Yang, Hejie Liu, Youbin Zheng, Hongyu Chu, Zhuming Lu, Jie Yuan, Shengshan Xu","doi":"10.2147/JIR.S509245","DOIUrl":"10.2147/JIR.S509245","url":null,"abstract":"<p><strong>Background: </strong>Nucleolar and spindle-associated protein 1 (PLIN3), a member of the perilipin family, plays a critical role in lipid droplet dynamics and is implicated in promoting tumor progression across several cancers. However, its influence on the tumor immune microenvironment and its potential as a prognostic indicator regarding immunotherapy responses have yet to be systematically evaluated. This study leverages data retrieved from multiple databases to address these questions.</p><p><strong>Methods: </strong>PLIN3 mRNA and protein expressions were analyzed across a diverse range of normal and cancerous tissues, utilizing data retrieved from multiple databases. The potential of PLIN3 as a diagnostic and prognostic biomarker in cancers was assessed. Advanced computational algorithms were employed to examine the impact of PLIN3 on immune cell infiltration. The association between PLIN3 expression and the presence of M2 macrophages was validated through analyses incorporating bulk and single-cell transcriptomics, spatial transcriptomics, and multicolor fluorescence staining techniques. Furthermore, the effects of PLIN3 on tumor malignancy and growth were investigated in vitro in lung adenocarcinoma (LUAD) cells. Potential compounds targeting PLIN3 were identified using the Connectivity Map (cMap) web tool, and their efficacy was further assessed through molecular docking.</p><p><strong>Results: </strong>PLIN3 was predominantly upregulated in various cancers, correlating with adverse prognostic outcomes. A strong positive association was observed between PLIN3 levels and M2 macrophage infiltration in several cancer types, establishing it as a potential pan-cancer marker for M2 macrophage presence. This was confirmed by integrative multi-omics analysis and multiple fluorescence staining. Additionally, PLIN3 knockdown in LUAD cells diminished their malignant traits, resulting in decreased proliferation and migration. In LUAD, clofibrate was identified as a potential inhibitor of PLIN3's pro-oncogenic functions.</p><p><strong>Conclusion: </strong>PLIN3 may serve as a potential biomarker and oncogene, particularly in LUAD. It plays a key role in mediating M2 macrophage infiltration in various cancers and presents a promising immunotherapeutic target.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"3757-3777"},"PeriodicalIF":4.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of CD53 Reduces the Formation of ROS-Induced Neutrophil Extracellular Traps and Protects Against Inflammatory Injury in Acute Pancreatitis.","authors":"Tianqi Xia, Fei Han, Yaning Wang, Xinyue Xie, Chenchen Yuan, Guotao Lu, Weiming Xiao, Bo Tu, Hongbo Ren, Weijuan Gong, Yaodong Wang","doi":"10.2147/JIR.S507886","DOIUrl":"10.2147/JIR.S507886","url":null,"abstract":"<p><strong>Background: </strong>The tetraspanin CD53 transmembrane protein is vital in immune cells like B cells and T cells, playing a crucial role in various inflammatory conditions. However, its involvement in neutrophils regarding inflammation remains uncertain. This study aims to examine the impact of CD53 on neutrophil extracellular traps (NETs) formation.</p><p><strong>Methods: </strong>Phorbol 12-myristate 13-acetate (PMA) was utilized to establish an in vitro classical NETs model to investigate the influence of CD53 on NETs formation and its regulatory mechanisms. Subsequently, the link between CD53 and acute pancreatitis (AP), a model of aseptic inflammatory responses connected to NETs, was verified. Peripheral blood neutrophils from clinical AP patients were collected to explore the role of CD53 in AP, while an AP mouse model induced by caerulein was employed to confirm the impact of CD53 inhibition on AP mice pancreatic tissue.</p><p><strong>Results: </strong>Our study has shown that CD53 is significantly elevated in in vitro NETs models and neutrophils from AP patients. The expression of CD53 is closely related to the clinical prognosis of AP patients. At the same time, CD53 neutralizing antibody (Anti-CD53) can significantly inhibit the formation of NETs in vitro, inflammatory injury in AP mice and the formation of NETs in damaged tissues. Mechanistically, CD53 can modulate the PI3K/AKT pathway and promote the formation of NETs. Finally, targeted regulation of CD53 can effectively reduce inflammatory injury and NETs formation in damaged tissues of AP mice.</p><p><strong>Conclusion: </strong>The results of this study mark the first confirmation that CD53 plays a crucial role in NETs formation. Targeting CD53 inhibition could potentially serve as a novel therapeutic approach for the treatment of AP.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"3725-3739"},"PeriodicalIF":4.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Serum LMAN2 and Sestrin2 in Septic Shock Patients and Exploration of Their Prognostic Value.","authors":"Zhen Chen, Zhenyu Chu, Limin Jia","doi":"10.2147/JIR.S501719","DOIUrl":"10.2147/JIR.S501719","url":null,"abstract":"<p><strong>Objective: </strong>The expressions and prognostic value of serum Lectin Mannose-Binding 2 (LMAN2) and Sestrin2 were evaluated in septic shock patients, aiming to provide new biomarkers for early diagnosis and prognosis judgment of septic shock patients.</p><p><strong>Methods: </strong>This retrospective study included 110 patients with sepsis and 50 healthy control subjects. Patients were classified into the sepsis group (SE group, 63 cases) or septic shock group (SS group, 47 cases) based on the occurrence of septic shock. Acute Physiology and Chronic Health Status II (APACHE II) scores, sequential organ failure assessment (SOFA) scores, and serum LMAN2 and Sestrin2 levels were compared between groups. The factors affecting the poor prognosis were analyzed by multivariate logistic regression. The receiver operating characteristic (ROC) curve was established to analyze the predictive value of serum LMAN2, Sestrin2, APACHE II score and SOFA score for the prognosis.</p><p><strong>Results: </strong>The serum LMAN2 levels in the SS group and SE group were significantly increased compared with the CON group, but the serum Sestrin2 levels were decreased (<i>P</i><0.05). The serum LMAN2 levels in the poor prognosis group were significantly higher than those in the good prognosis group, while the Sestrin2 levels were significantly decreased (<i>P</i><0.05). Serum level of LMAN2, APACHE II scores and SOFA scores were independent risk factors, but Sestrin2 level was protective factor (<i>P</i><0.05). Meanwhile, the AUC of serum LMAN2 and Sestrin2 combined detection was 0.894, and the specificity and sensitivity were 93.33% and 84.38%, respectively, which had high predictive value for the prognosis of septic shock patients. The AUC of serum LMAN2 and Sestrin2 combined with APACHE II score and SOFA score was 0.960, the specificity was 93.75%, and the sensitivity was 86.67%. Compared with the detection alone, the AUC of combined detection was increased (Z =-2.166, -2.758, -2.059, -2.172, <i>P</i><0.05).</p><p><strong>Conclusion: </strong>The increase of serum LMAN2 levels and the decrease of Sestrin2 levels were closely related to the severity of septic shock. The combined detection had important predictive value for the prognosis of septic shock patients. This study may have the potential to improve the management and treatment of sepsis patients.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"3713-3724"},"PeriodicalIF":4.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11912917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Inflammation Indices Derived From Complete Blood Count and Coronary Artery Calcification.","authors":"Yi He, Lian Li, Ting Zhou, Hao Yang, Tao Liu, Houyuan Hu","doi":"10.2147/JIR.S501429","DOIUrl":"10.2147/JIR.S501429","url":null,"abstract":"<p><strong>Background: </strong>Inflammation plays an important role in the pathogenesis of coronary artery calcification (CAC). This study aims to explore the potential association between inflammation indices derived from complete blood count (CBC) and CAC, including the neutrophil to lymphocyte ratio (NLR), derived neutrophil to lymphocyte ratio (dNLR), neutrophil-monocyte to lymphocyte ratio (NMLR), systemic inflammation response index (SIRI), systemic immune-inflammation index (SII), aggregate index of systemic inflammation (AISI), platelet to lymphocyte ratio (PLR), and monocyte to lymphocyte ratio (MLR).</p><p><strong>Methods: </strong>We systematically collected data from patients who underwent CAC scoring via cardiac CT at our hospital between July 2018 and June 2023. Patients were divided into two groups based on the presence or absence of CAC. Multivariate logistic regression analysis, smooth curve fitting, and threshold effect analysis were subsequently used to explore the potential linear or nonlinear relationships between CBC-derived inflammation indices and CAC. Subgroup analyses were conducted to examine the consistency of these findings across different subgroups.</p><p><strong>Results: </strong>A total of 2143 participants were included in this study: the CAC group (1286 participants) and the non-CAC group (857 participants). In the four subgroups of CAC, within-group comparisons revealed that alkaline phosphatase (ALP), smoking status, and peripheral artery plaques were more prevalent in the group with CAC scores > 400. After adjusting for confounding variables, we found that the total NLR, NMLR, SIRI, and AISI were positively associated with CAC. Subsequently, we identified a nonlinear relationship between MLR and CAC, with a threshold value of 0.236. Additionally, subgroup analysis indicated that these associations remained stable across various subgroups.</p><p><strong>Conclusion: </strong>This study indicates that the total NLR, NMLR, SIRI, and AISI are significantly positively correlated with CAC in a linear association, while MLR exhibits a nonlinear relationship with CAC. In contrast, SII, PLR, and dNLR show no significant association with CAC.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"3807-3816"},"PeriodicalIF":4.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Lipid Biomarkers for the Diagnosis and Monitoring of Neuromyelitis Optica Spectrum Disorder: Towards Improved Clinical Management.","authors":"Ruibing Li, Jinyang Wang, Jianan Wang, Wei Xie, Pengfei Song, Jie Zhang, Yun Xu, Decai Tian, Lei Wu, Chengbin Wang","doi":"10.2147/JIR.S496018","DOIUrl":"10.2147/JIR.S496018","url":null,"abstract":"<p><strong>Background: </strong>Neuromyelitis optica spectrum disorder (NMOSD) is a group of immune-mediated disorders that often lead to severe disability. The diagnosis and monitoring of NMOSD can be challenging, particularly in seronegative cases, highlighting the need for reliable biomarkers to enhance clinical management. This study aimed to identify serum lipid biomarkers for the diagnosis and monitoring of NMOSD and to assess their potential to improve clinical decision-making.</p><p><strong>Methods: </strong>We conducted a comprehensive serum proteomic analysis in a discovery cohort of NMOSD patients and controls to identify lipid-related proteins associated with NMOSD. Subsequently, we validated the candidate biomarkers in the retrospective cohort and developed diagnostic models using a random forest algorithm. The association between these lipid biomarkers and disease activity was further evaluated in longitudinal analysis.</p><p><strong>Results: </strong>Our analysis identified a panel of serum lipid-related biomarkers that demonstrated significant differences between NMOSD patients and controls. The diagnostic models achieved the impressive accuracy of 72% for the full NMOSD spectrum, 72% for AQP4-IgG+ NMOSD, and 68% for double seronegative NMOSD. Importantly, these biomarkers showed a correlation with disease activity, with levels changing from relapse to remission. Additionally, a combination of these lipid biomarkers was found to predict relapse with the AUC of 0.861. A user-friendly smartphone application was developed to facilitate the straightforward \"input-index, output-answer\" screening process, enhancing both clinical decision-making and patient care.</p><p><strong>Conclusion: </strong>The diagnostic model based on the serum lipid-related indexes (TC, TG, LDL, HDL, ApoA1, and ApoB) may be the useful tool for NMOSD in diagnosis and monitoring of disease stage, thereby improving the treatment outcome for patients. Future studies should focus on integrating these biomarkers into routine clinical practice to realize their full potential in enhancing NMOSD management.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"3779-3794"},"PeriodicalIF":4.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}