Journal of Inflammation Research最新文献

{"title":"Characterization of Mucosal Immune-Related lncRNAs and mRNAs in a Mouse Model of Allergic Conjunctivitis.","authors":"Hong Zhang, Hongyu Zhang, Qing Leng, Ya Juan Zheng","doi":"10.2147/JIR.S511048","DOIUrl":"https://doi.org/10.2147/JIR.S511048","url":null,"abstract":"<p><strong>Background: </strong>Allergic conjunctivitis (AC) is a common inflammatory condition characterized by immune dysregulation in response to environmental allergens. Despite extensive research into general allergic mechanisms, the specific immunological features of the ocular mucosal microenvironment remain poorly understood. Investigating immune-related mRNAs and LncRNAs may provide insights into the mechanisms underlying AC and potential novel targets for therapeutic intervention.</p><p><strong>Methods: </strong>An AC model was established using female BALB/c mice sensitized with ragweed pollen. Conjunctival tissues from AC and control groups were pooled for RNA extraction, followed by Illumina sequencing. Differential gene expression was identified using DESeq2, and functional enrichment was analyzed using GO, KEGG, and GSEA. RT-qPCR validated results, while the Human Protein Atlas was used to assess protein expression.</p><p><strong>Results: </strong>A murine model of AC was successfully established, confirmed by progressively increasing clinical scores and significantly elevated scratching frequency. Transcriptomic analysis revealed significant differences in mRNAs and lncRNAs expression between AC and control groups. GO analysis indicated that both upregulated and downregulated genes were enriched in biological processes related to response to stimulus, immune system processes, signaling, and metabolic processes. KEGG analysis showed that upregulated genes were enriched in pathways such as steroid hormone biosynthesis, histidine metabolism, glycolysis/gluconeogenesis, and IL-17 signaling, while downregulated genes were involved in cytokine-cytokine receptor interaction and hematopoietic cell lineage. GSEA identified significant enrichment in inflammatory pathways, including MAPK, STAT1, and STAT2. Mucosal immunity-related genes such as Bpifa1, Lcn2, and Reg3g were upregulated in AC. Co-expression analysis also revealed several upregulated lncRNAs, including Stoml3-202 and Etohd2-205.</p><p><strong>Conclusion: </strong>This study is the first to systematically analyze immune-related mRNAs and LncRNAs in AC, identifying mucosal immunity molecules like Bpifa1 and Reg3g. These findings underscore the unique involvement of mucosal immunity in AC and provide potential new targets for immune modulation in ocular allergy treatment.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6061-6076"},"PeriodicalIF":4.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting Superaverage Length of Stay in COPD Patients with Hypercapnic Respiratory Failure Using Machine Learning.","authors":"Bingqing Zuo, Lin Jin, Zhixiao Sun, Hang Hu, Yuan Yin, Shuanying Yang, Zhongxiang Liu","doi":"10.2147/JIR.S511092","DOIUrl":"https://doi.org/10.2147/JIR.S511092","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of this study was to develop and validate machine learning models that can predict superaverage length of stay in hypercapnic-type respiratory failure and to compare the performance of each model. Furthermore, screen and select the optimal individualized risk assessment model. This model is capable of predicting in advance whether an inpatient's length of stay will exceed the average duration, thereby enhancing its clinical application and utility.</p><p><strong>Methods: </strong>The study included 568 COPD patients with hypercapnic respiratory failure, 426 inpatients from the Department of Respiratory and Critical Care Medicine of Yancheng First People's Hospital in the modeling group and 142 inpatients from the Department of Respiratory and Critical Care Medicine of Jiangsu Provincial People's Hospital in the external validation group. Ten machine learning algorithms were used to develop and validate a model for predicting superaverage length of stay, and the best model was evaluated and selected.</p><p><strong>Results: </strong>We screened 83 candidate variables using the Boruta algorithm and identified 9 potentially important variables, including: cerebrovascular disease, white blood cell count, hematocrit, D-dimer, activated partial thromboplastin time, fibrin degradation products, partial pressure of carbon dioxide, reduced hemoglobin, and oxyhemoglobin. Cerebrovascular disease, hematocrit, activated partial thromboplastin time, partial pressure of carbon dioxide, reduced hemoglobin and oxyhemoglobin were independent risk factors for superaverage length of stay in COPD patients with hypercapnic respiratory failure. The Catboost model is the optimal model on both the modeling dataset and the external validation set. The interactive web calculator was developed using the Shiny framework, leveraging a predictive model based on Catboost.</p><p><strong>Conclusion: </strong>The Catboost model has the most advantages and can be used for clinical evaluation and patient monitoring.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5993-6008"},"PeriodicalIF":4.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068394/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Influence and Mechanism of Resveratrol on Cognitive Impairment in Chronic Kidney Disease Rats Through Regulating Gut Microbiota and the TLR4/NFκB Pathway.","authors":"Binbin Shao, Yanfei Nong, Yongshuang Lin, Yan Meng, Yi Zhou, Meiying Huang, Feifan Huang, Jie Wang","doi":"10.2147/JIR.S510867","DOIUrl":"https://doi.org/10.2147/JIR.S510867","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the mechanism by which resveratrol (Res) ameliorates cognitive impairment (CI) in chronic kidney disease (CKD) rats through modulation of gut microbiota and suppression of inflammation.</p><p><strong>Methods: </strong>A CKD model was established in rats via two intravenous injections of doxorubicin (4 mg/kg, 2 weeks apart). After 8 weeks, renal function and histopathological assessments were performed to confirm the establishment of the CKD model.Rats were divided into control, CKD, and CKD+Res groups. The CKD+Res group received intragastric Res for 6 weeks. Cognitive function was assessed using the Morris water maze. Serum Interleukin-6 (IL-6), Tumor Necrosis Factor-alpha (TNF-α), and Lipopolysaccharide (LPS) levels were measured via ELISA. Histopathology evaluated kidney, colon, and hippocampal damage. Gut microbiota composition was analyzed by 16S rRNA sequencing, and hippocampal Toll-Like Receptor 4 (TLR4)/ the Nuclear Factor-κB (NFκB) pathway proteins were quantified via Western blot.</p><p><strong>Results: </strong>CKD groups exhibited elevated 24-hour urinary albumin, serum urea nitrogen, and creatinine (<i>P</i> < 0.01), with glomerular atrophy. During water maze navigation (days 3-4), CKD groups showed prolonged escape latency and increased swimming distance versus controls (<i>P</i> < 0.05), which Res intervention alleviated (<i>P</i> < 0.05). In the spatial probe test, CKD rats had fewer platform crossings and shorter target quadrant occupancy (<i>P</i> < 0.01; <i>P</i> < 0.05), both improved by Res (<i>P</i> < 0.05). Hippocampal neuronal damage and elevated serum IL-6, TNF-α, and LPS levels (<i>P</i> < 0.01) were observed in CKD rats, while Res reduced IL-6 and LPS (<i>P</i> < 0.05). Western blot revealed upregulated TLR4/NFκB pathway activation in the CKD group (<i>P</i> < 0.01), suppressed by Res (<i>P</i> < 0.05). Gut microbiota analysis showed increased Gram-negative bacteria in CKD rats and higher Gram-positive bacteria abundance in the Res group. LPS biosynthesis was enhanced in CKD rats (<i>P</i> < 0.05) but attenuated by Res.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6049-6060"},"PeriodicalIF":4.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068408/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Mechanism of Action of Xuebijing Injection in Treating Acute Respiratory Distress Syndrome Based on Network Pharmacology and Animal Experiments.","authors":"Jin Li, Meirong Shen, Zuan Yin","doi":"10.2147/JIR.S507468","DOIUrl":"https://doi.org/10.2147/JIR.S507468","url":null,"abstract":"<p><strong>Objective: </strong>To explore the mechanism of Xuebijing injection (XBJ) in treating acute respiratory distress syndrome (ARDS) using network pharmacology and animal experiments.</p><p><strong>Methods: </strong>Active ingredients of XBJ were analyzed via TCMSP, and ARDS-related targets were identified through DisGENET and Genecard. Intersection targets were obtained using PubChem and Venn diagrams. Protein interaction networks, GO, and KEGG enrichment analyses were conducted. An ARDS rat model was established using lipopolysaccharide (LPS), and rats were divided into control, LPS, and XBJ-treated groups (low, medium, high doses, n=10). Lung wet/dry (W/D) ratio, inflammatory cytokines (IL-17, IL-6, IL-1β, TNF-α), MPO levels, lung pathology, and protein expression of ICAM-1 and HIF-1α were assessed via ELISA, HE staining, immunohistochemistry, and Western blot.</p><p><strong>Results: </strong>A total of 204 ARDS targets were identified, with 46 intersection targets, mainly TNF-α, MPO, HIF-1α, and ICAM-1. XBJ affected ARDS through IL-17, HIF-1, and TNF signaling pathways. In vivo, LPS-induced lung injury showed alveolar destruction, edema, and inflammation, with increased W/D ratio, cytokines, MPO, and protein expression of HIF-1α and ICAM-1 (P<0.05). XBJ treatment alleviated lung damage, reduced inflammation, and improved pathology in a dose-dependent manner (P<0.05).</p><p><strong>Conclusion: </strong>XBJ alleviates ARDS by regulating immune function, oxidative stress, and inflammation through IL-17, HIF-1, and TNF pathways.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6037-6047"},"PeriodicalIF":4.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12067646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can Systemic Inflammatory Markers Be Used in Pulmonary Embolism Risk Assessment in Patients with Acute Pulmonary Thromboembolism?","authors":"Levent Özdemir, Burcu Özdemir, Savaş Gegin, Esra Arslan Aksu, Ahmet Cemal Pazarlı","doi":"10.2147/JIR.S514111","DOIUrl":"https://doi.org/10.2147/JIR.S514111","url":null,"abstract":"<p><strong>Background: </strong>Understanding the effect of systemic inflammation on the pathophysiology of thromboembolism may provide an approach to determine the course and prognosis of the disease. The aim of this study was to investigate the usability of systemic inflammatory markers in the risk stratification of pulmonary embolism in patients with acute pulmonary thromboembolism (PTE).</p><p><strong>Methods: </strong>The data of 234 patients diagnosed with pulmonary embolism by computed tomography pulmonary angiography (CTPA) or ventilation perfusion scintigraphy were evaluated retrospectively. Demographic data, co-morbid conditions, and laboratory parameters of the patients were obtained from the hospital data system. Pulmonary embolism risk classification was performed according to the 2019 ESC guidelines as low, intermediate (intermediate-low, intermediate-high), and high risk. Neutrophil - lymphocyte ratio (NLR), platelets-to-lymphocyte ratio (PLR), lymphocyte - monocyte ratio (LMR), lymphocyte / CRP ratio (LCRPR), systemic inflammatory response index (SIRI) (Neutrophil×Platelet/Lymphocyte) and systemic immune-inflammation index (SII) (Neutrophil×Monocyte/Lymphocyte) were calculated using the patients' hemogram (White blood count (WBC), hemoglobin, hematocrit, platelet, neutrophil, lymphocyte, monocyte), C-reactive protein (CRP), lactate, troponin, and d-dimer values at the time of diagnosis.</p><p><strong>Results: </strong>In our study, WBC, neutrophils, NLR, PLR, SIRI, SII and CRP levels were significantly lower in low risk, while lymphocyte count and LCRPR were significantly higher. Platelet counts were significantly lower in high risk. D-dimer levels were significantly higher in intermediate-high and high risk. Lactate levels were significantly higher in high risk. Troponin levels were significantly higher in intermediate-high risk and high risk. WBC, neutrophils, D-dimer, troponin, lactate levels and NLR, SII, indices were found to be significant biomarkers in predicting high-risk embolism.</p><p><strong>Conclusion: </strong>Our findings suggest that systemic inflammatory markers may be a clinically important risk determinant in patients with acute pulmonary thromboembolism.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5969-5977"},"PeriodicalIF":4.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12067453/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Significance of the Serum 3'tRF-AlaAGC, Neutrophil to High-Density Lipoprotein Ratio, and Lymphocyte-to-Monocyte Ratio in Breast Cancer with Lymph Node Metastasis.","authors":"Dongping Mo, Yurong Zhu, Xuelian Mao, Cong Li, Yining Yang, Junyu Zheng, Feng Yan","doi":"10.2147/JIR.S518232","DOIUrl":"https://doi.org/10.2147/JIR.S518232","url":null,"abstract":"<p><strong>Objective: </strong>Breast cancer (BC) is a common malignant tumor among women, the local recurrence, lymph node metastasis (LNM), and distant metastasis are the key factors affecting the prognosis of patients. tRNA-derived small RNAs (tDRs) are non-coding small RNA fragments [16-40 nucleotides (nt) in length] that play an important role in carcinogenesis and can serve as novel biological markers for the diagnosis and prognosis of various tumors. Accumulating evidence suggests that blood-based inflammatory indicators are linked with the pathogenesis of BC. However, the clinical significance of the combination of tDRs and inflammatory indicators in BC patients with LNM is still unclear.</p><p><strong>Methods: </strong>The serum samples were collected from 175 patients with BC admitted to our hospital during June 2021 and May 2024, and 94 age-matched healthy women, and the clinical data of the research subjects were recorded. Serum 3'tRF-AlaAGC levels were measured using quantitative real-time PCR (qRT-PCR) and the blood-based inflammatory indicators were calculated from peripheral blood samples. Lasso-cox regression and multiple logistic regression were employed for variable selection. Receiver operating characteristic (ROC) was used to calculate the cut-off value of variables. Spearman correlation test was used to examine the correlation between 3'tRF-AlaAGC levels and neutrophil to HDL-C ratio (NHR), lymphocyte-to-monocyte ratio (LMR). A nomogram model for risk assessment of LNM in BC was established by using the rms package of R software.</p><p><strong>Results: </strong>Serum 3'tRF-AlaAGC levels in BC patients with LNM were significantly higher than that in without LNM [5.17 (1.79, 16.55) vs 11.68 (2.64, 58.74), <i>P</i>=0.009]. The variables screened by Lasso-cox regression including 3'tRF-AlaAGC, NHR and LMR, with optimal cut-off values of 18.78, 2.94 and 5.41, respectively. NHR levels were significantly negatively associated with LMR in low 3'tRF-AlaAGC expression groups (<i>r</i>=-0.209, <i>P</i>=0.021). Multivariate logistic regression analysis confirmed that 3'tRF-AlaAGC (OR: 3.242, 95% CI: 1.583-6.641, <i>P</i>=0.001), NHR (OR: 3.305, 95% CI: 1.543-7.079, <i>P</i>=0.002), and LMR (OR: 0.329, 95% CI: 0.150-0.723, <i>P</i>=0.006) were independent risk factors of BC with LNM. The C-statistic of the nomograms model was 0.704, with a sensitivity of 57.14% and a specificity of 77.14%.</p><p><strong>Conclusion: </strong>3'tRF-AlaAGC >18.78, NHR > 2.94, and LMR ≤ 5.41 were the independent risk factors of BC with LNM. The nomogram model incorporating 3'tRF-AlaAGC, NHR and LMR can effectively predict the risk of LNM of BC patients.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5979-5991"},"PeriodicalIF":4.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12067465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Gut Microbiota on Intestinal Fibrosis in Inflammatory Bowel Disease and Traditional Chinese Medicine Intervention.","authors":"Leyao Fang, Huiyi Peng, Zhoujin Tan, Na Deng, Xinxin Peng","doi":"10.2147/JIR.S504827","DOIUrl":"https://doi.org/10.2147/JIR.S504827","url":null,"abstract":"<p><p>Inflammatory bowel disease (IBD) is a chronic, relapsing inflammatory disorder of the intestine, frequently complicated by intestinal fibrosis. As fibrosis progresses, it can result in luminal stricture and compromised intestinal function, significantly diminishing patients' quality of life. Emerging evidence suggests that gut microbiota and their metabolites contribute to the pathogenesis of IBD-associated intestinal fibrosis by influencing inflammation and modulating immune responses. This review systematically explores the mechanistic link between gut microbiota and intestinal fibrosis in IBD and evaluates the therapeutic potential of traditional Chinese medicine (TCM) interventions. Relevant studies were retrieved from PubMed, Web of Science, Embase, Scopus, CNKI, Wanfang, and VIP databases. Findings indicate that TCM, including Chinese herbal prescriptions and bioactive constituents, can modulate gut microbiota composition and microbial metabolites, ultimately alleviating intestinal fibrosis through anti-inflammatory, immunemodulatory, and anti-fibrotic mechanisms. These insights highlight the potential of TCM as a promising strategy for targeting gut microbiota in the management of IBD-associated fibrosis.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5951-5967"},"PeriodicalIF":4.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12067688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shared and Distinctive Inflammation-Related Protein Profiling in Idiopathic Inflammatory Myopathy with/without Anti-MDA5 Autoantibodies.","authors":"Yusheng Zhang, Wenlu Hu, Tianqi Li, Zhou Pan, Jinlei Sun, Yujie He, Wenjuan Guan, Lijuan Zhang, Chaofeng Lian, Shengyun Liu, Panpan Zhang","doi":"10.2147/JIR.S509777","DOIUrl":"https://doi.org/10.2147/JIR.S509777","url":null,"abstract":"<p><strong>Purpose: </strong>Due to the heterogeneous nature of the diseases, treatment efficacy and prognosis vary for idiopathic inflammatory myopathy (IIM) patients with different myositis specific autoantibodies (MSAs). This study aimed to investigate the inflammation-related protein profiling of IIM patients with different MSAs. In addition, the shared and distinctive inflammation-related protein profiling in IIM with/without anti-MDA5 autoantibodies.</p><p><strong>Methods: </strong>Seventy-seven patients with IIM of different MSAs and 53 gender/age matched healthy controls (HCs) were enrolled in this study. Ninety-two inflammation-related proteins were detected by Olink proteomics. We identified differentially expressed proteins (DEPs), and performed gene set enrichment analysis and KEGG pathway analysis. In addition, correlation between DEPs and serological parameters were performed. The least absolute shrinkage and selection operator (Lasso) regression algorithm of machine learning was used to screen biomarkers related to anti-MDA5+ DM.</p><p><strong>Results: </strong>Compared with HCs, 36 inflammation-related proteins were identified as DEPs. The top 10 DEPs were CXCL10, CXCL11, CXCL9, CXCL8, S100A12, IL-6, CCL2, CCL8, IL-10 and CCL3. The inflammation-related proteins and cytokine-cytokine receptor interaction pathway were more strikingly expressed in patients with anti-MDA5+ DM patients than in anti-MDA5- IIM patients. In addition, multiple DEPs correlated with serum ferritin, KL-6, muscle enzymes. For the first time, we established that a multi-factor panel comprising CX3CL1, IL-17C, IL-18R1, CCL20, and TNF (AUC = 0.824) serves as a highly efficient diagnostic biomarker for anti-MDA5+ DM.</p><p><strong>Conclusion: </strong>Plasma profiling revealed that inflammation and inflammatory pathways were extremely elevated in patients with IIM, especially in patients with anti-MDA5 autoantibodies. The shared and distinctive inflammation-related protein signature was demonstrated in patients with/without anti-MDA5 autoantibodies. The expression of CX3CL1 was significantly higher in anti-MDA5+ DM than in patients without anti-MDA5 autoantibodies. In addition, CX3CL1 correlated with ESR, serum ferritin, CK enzymes and disease activity, indicating that CX3CL1 participated in inflammation status of anti-MDA5+ DM.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6009-6024"},"PeriodicalIF":4.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12067647/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Utility of Pentraxin 3 Expression in Septic Cardiomyopathy: Findings from a Prospective Observational Study.","authors":"Na Cui, Xiao-Wei Lv, Teng-Hao Shao, Zhan-Biao Yu, Zhi Chen, Tao Sun, Tie-Min Li, Jin-Wen Zhang, Zhen-Jie Hu","doi":"10.2147/JIR.S517566","DOIUrl":"https://doi.org/10.2147/JIR.S517566","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study is to investigate the expression levels of Pentraxin 3, PTX3 in patients with septic cardiomyopathy, SCM and evaluate its diagnostic potential for predicting SCM.</p><p><strong>Methods: </strong>A prospective observational study was conducted involving 122 patients diagnosed with septic shock between February 2023 and August 2024. Demographic and clinical data, along with plasma PTX3 concentrations were recorded. Participants were categorized into two groups based on the presence of SCM. PTX3 concentrations and their dynamic changes were compared between the groups. The correlations between PTX3 concentrations and other clinical indicators were analyzed, and the influencing factors associated with SCM development were assessed. The predictive performance of PTX3 for SCM was evaluated using receiver operating characteristic, ROC curves.</p><p><strong>Results: </strong>SCM was identified in 24.6% of the participants. Plasma PTX3 concentrations at admission and on day 3 were significantly higher in the SCM group compared to the non-SCM group, <i>p</i> < 0.001. However, no significant differences were observed on day 7, <i>p</i> > 0.05. PTX3 concentrations decreased over time in both groups. Plasma PTX3 concentrations were correlated with procalcitonin, lactate, myoglobin, troponin I, the elevated levels of troponin I on day 2 compared to admission, Sequential Organ Failure Assessment score, Acute Physiology and Chronic Health Evaluation II score, and intensive care unit length of stay. Elevated plasma PTX3 concentrations were identified as an independent risk factor for SCM development. The area under the ROC curve for PTX3 in predicting SCM was 0.784, <i>p</i> < 0.001, with a cutoff value of 20.82 ng/mL, sensitivity of 0.767, and specificity of 0.707.</p><p><strong>Conclusion: </strong>Plasma PTX3 concentrations are markedly elevated in patients with SCM, indicating that PTX3 may serve as a reliable biomarker for the early diagnosis of SCM.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"6025-6035"},"PeriodicalIF":4.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12067461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of Acetate in Alleviating SETDB1-Linked Neuroinflammation and Cognitive Impairment in a Mouse Model of OSA.","authors":"Zhan Zhao, Li Xiang, Jau-Shyong Hong, Yubao Wang, Jing Feng","doi":"10.2147/JIR.S510690","DOIUrl":"https://doi.org/10.2147/JIR.S510690","url":null,"abstract":"<p><strong>Background: </strong>Microglia-mediated neuroinflammation is crucial for obstructive sleep apnea (OSA)-induced cognitive impairment. We aimed to investigate roles of acetate (ACE) and SET domain bifurcated histone lysine methyltransferase 1 (SETDB1) in neuroinflammation of OSA.</p><p><strong>Methods: </strong>After C57BL/6J mice were exposed to OSA-associated intermittent hypoxia (IH) or normoxia for four weeks, the composition of the gut microbiota (GM) and the levels of serum short-chain fatty acids (SCFAs) were measured by 16S rRNA and GC-MS methods, respectively. To assess the effect of ACE on IH mice, glyceryl triacetate (GTA) was gavaged in IH-exposed mice and the cognitive function, microglial activation, and hippocampal neuronal death were examined. Moreover, ACE-treated BV2 microglia cells were also utilized for further mechanistic studies.</p><p><strong>Results: </strong>IH disrupts the gut microbiome, reduces microbiota-SCFAs, and impairs cognitive function. Gavage with GTA significantly mitigated these cognitive deficits. Following IH exposure, we observed substantial increases in SETDB1 both in vivo and in vitro, along with elevated levels of histone H3 lysine 9 trimethylation (H3K9me3). Genetic or pharmacological inhibition of SETDB1 in microglia led to decreased induction of proinflammatory factors, as well as reduced reactive oxygen species (ROS) generation. Mechanistically, SETDB1 was found to upregulate the transcription factors p-signal transducer and activator of transcription 3 (p-STAT3) and p-NF-κB. In vitro, ACE supplementation effectively repressed high SETDB1 and H3K9me3 levels, thereby inhibiting microglial pro-inflammatory responses induced by IH. In vivo, ACE supplementation significantly reduced hippocampal levels of p-STAT3, p-NF-κB, and pro-inflammatory cytokines while also protecting neuronal integrity.</p><p><strong>Conclusion: </strong>This study provides the first evidence that H3K9 methyltransferase SETDB1 promotes microglial pro-inflammatory response distinct from its previously shown role in macrophages. Our findings also identify ACE supplementation as a promising dietary intervention for OSA-related cognitive impairment with SETDB1 serving as both a mechanistic biomarker and potential therapeutic target.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5931-5950"},"PeriodicalIF":4.2,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12067661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}