Journal of Inflammation Research最新文献

{"title":"IL-22 Alleviates Sepsis-Induced Acute Lung Injury by Inhibiting Epithelial Cell Apoptosis Associated with STAT3 Signalling.","authors":"Chiying Zhu, Jiabo Chen, Zhengzheng Yan, Fei Wang, Ziqi Sun, Zeyu Liu, Ying Li, Xiaona Chen, Ziwei Bao, Quan Li, Zhixia Chen","doi":"10.2147/JIR.S496387","DOIUrl":"https://doi.org/10.2147/JIR.S496387","url":null,"abstract":"<p><strong>Purpose: </strong>Sepsis is a critical condition characterized by organ dysfunction due to an aberrant response to infection, which results in a life-threatening situation. The lung, which is the most vulnerable target organ, is often severely damaged during sepsis. Research has demonstrated that interleukin-22 (IL-22), which is secreted by various immunocytes, can mitigate inflammation-associated diseases. Nevertheless, the precise function of IL-22 in sepsis-induced acute lung injury (SALI) is still unclear. This study aimed to investigate the therapeutic efficacy of IL-22 in sepsis and explore the regulatory mechanisms involved.</p><p><strong>Methods: </strong>A mouse caecal ligation and puncture (CLP) model of sepsis was established, and the effect of IL-22 was investigated as indicated. Immunohistochemistry, qRT‒PCR, ELISA, immunofluorescence, TUNEL, Western blotting, and flow cytometry assays were applied to investigate the protective efficacy and involved pathways. Additionally, an in vitro model of lipopolysaccharide (LPS)-induced bronchial epithelial cell (BEAS-2B) apoptosis was established, and these cells were treated with or without recombinant IL-22 (rIL-22) to further evaluate the effect of IL-22 and the underlying mechanism.</p><p><strong>Results: </strong>The experimental results clearly confirmed that the levels of IL-22 were increased in the serum and lung tissue after CLP. The administration of rIL-22 was observed to increase the survival rate of septic mice. Notably, rIL-22 treatment resulted in decreased levels of proteins and a decreased cell number in the bronchoalveolar lavage fluid, as well as in a reduction in inflammatory cytokine release into the serum. Importantly, rIL-22 mitigated SALI by inhibiting lung cell apoptosis in septic mice. Furthermore, the results revealed that rIL-22 attenuated apoptosis of lung epithelial cells via the activation of the STAT3 signalling pathway.</p><p><strong>Conclusion: </strong>The results of this study suggest that IL-22 alleviates lung epithelial cell apoptosis to protect mice against SALI in association with the STAT3 signalling pathway, highlighting the potential therapeutic value of IL-22 against sepsis.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5383-5398"},"PeriodicalIF":4.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12024478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fumarate Hydratase Restrains mtDNA Attenuates LPS-Induced Acute Lung Injury Through cGAS-STING Pathways.","authors":"Zewen Jiang, Ruyuan He, Yujian Zhong, Bohao Liu, Ziqi He","doi":"10.2147/JIR.S518589","DOIUrl":"https://doi.org/10.2147/JIR.S518589","url":null,"abstract":"<p><strong>Background: </strong>The metabolic reprogramming of alveolar macrophages, particularly mitochondrial energy metabolism centered on the tricarboxylic acid (TCA) cycle, plays a pivotal role in acute lung injury (ALI). Fumarate hydratase (FH), a key enzyme catalyzing fumarate-to-malate conversion in the TCA cycle, is implicated in macrophage inflammatory responses, but its specific role in ALI remains unclear.</p><p><strong>Methods: </strong>We employed FHIN1 to assess its regulatory effects in LPS-induced ALI models. Wildtype C57BL/6 mice were randomly divided into control group, FHIN1 group, LPS group and LPS+FHIN1 group. FHIN1 and RU.521 was used to explored the interaction of FH and cGAS-STING in THP-1 cells.</p><p><strong>Results: </strong>LPS stimulation suppressed FH expression and induced fumarate accumulation in macrophages. Pharmacological FH inhibition exacerbated LPS-triggered inflammatory cytokine release, oxidative stress and aggravated lung injury in mice. Mechanistically, FH inhibition promoted mtDNA leakage, activating the cGAS-STING pathway to amplify inflammation. Blocking cGAS with RU.521 significantly attenuated FHIN1-driven inflammatory responses and mitigated lung injury exacerbation.</p><p><strong>Conclusion: </strong>FH critically modulates ALI progression by restraining cGAS-STING-dependent inflammation. Targeting the FH-mtDNA-cGAS axis may offer therapeutic potential for ALI management.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5399-5413"},"PeriodicalIF":4.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12024470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cervical Conization and Systemic Inflammatory Markers: The Predictive Value of Neutrophil Percentage to Albumin Ratio (NPAR) to Identify High-Grade Cervical Intraepithelial Neoplasia (CIN).","authors":"Sıtkı Özbilgeç, Fatih Akkuş","doi":"10.2147/JIR.S512991","DOIUrl":"https://doi.org/10.2147/JIR.S512991","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the predictive value of systemic inflammatory markers, particularly the Neutrophil Percentage to Albumin Ratio (NPAR), in identifying high-grade cervical intraepithelial neoplasia (CIN) in patients undergoing colposcopy and cervical conization.</p><p><strong>Materials and methods: </strong>A retrospective analysis was conducted on 116 patients who underwent cervical conization between January 2020 and May 2024. Demographic, clinical, and laboratory data were collected, and inflammatory indices, including NPAR, NLR (Neutrophil-to-Lymphocyte Ratio), PLR (Platelet-to-Lymphocyte Ratio), and SII (Systemic Immune-Inflammation Index), were calculated. Sample size estimation was based on prior studies assessing inflammatory markers in CIN, ensuring adequate statistical power for detecting differences in biomarker levels. ROC curve analysis was performed to assess the diagnostic accuracy of these markers.</p><p><strong>Results: </strong>NPAR demonstrated the highest predictive value for high-grade CIN, with an AUC of 0.893. Significant correlations were found between NPAR and other systemic inflammatory markers, such as NLR and SII. However, NLR and PLR showed lower predictive accuracy compared to NPAR.</p><p><strong>Conclusion: </strong>NPAR is a valuable biomarker for predicting high-grade CIN and can aid in patient stratification and treatment planning. Integrating NPAR with other systemic markers may enhance the accuracy of clinical decisions. Further studies with larger cohorts are recommended to validate these findings and explore their clinical utility.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5343-5353"},"PeriodicalIF":4.2,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12017268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the Mechanism of Artesunate in Modulating AR Epithelial Injury and Th2-Type Inflammatory Status.","authors":"Youwei Bao, Zhiqiang Zhang, Binbin Shi, Qi Chen, Ying Zhang, Xinhua Zhu","doi":"10.2147/JIR.S513760","DOIUrl":"https://doi.org/10.2147/JIR.S513760","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the therapeutic efficacy of Artesunate (ART) in a mouse model of allergic rhinitis (AR) induced by house dust mite (HDM) and explore its underlying mechanism.</p><p><strong>Experimental methods: </strong>Transcriptome sequencing (RNA-seq) analysis identified differentially expressed genes (DEGs) in nasal mucosa between healthy and allergic mice, with Gene Set Enrichment Analysis (GSEA) revealing STING pathway activation. We established a house dust mite (HDM)-induced allergic rhinitis (AR) mouse model via intraperitoneal sensitization. Artesunate (ART) efficacy was evaluated through dose-response testing (10-30 mg/kg), with 30 mg/kg identified as the optimal therapeutic dose. Mice were stratified into four groups: normal control (NC), NC+ART, AR model, and AR+ART-treated. Interventions were administered intraperitoneally, followed by systematic evaluation of: ① behavioral symptoms (sneezing/nasal scratching), ② histopathological changes in nasal and lung tissues, ③ serum TH2 cytokine levels, and ④ nasal mucosal protein expression profiles.</p><p><strong>Results: </strong>With increasing concentrations of Artesunate (10, 20, 30 mg/kg), there was a significant improvement in the TH2 inflammatory status in AR mice. The cGAS-STING signaling pathway determines the degree of epithelial tissue inflammatory damage and systemic TH2-type inflammatory status in AR mice. Artesunate inhibits the cGAS-STING signaling pathway, protects the mitochondrial structure of epithelial tissue in AR mice, and improves epithelial damage and systemic TH2-type inflammatory status.</p><p><strong>Conclusion: </strong>This study presents a new treatment approach for respiratory allergies by clarifying how Artesunate (ART) alleviates allergic rhinitis, identifying effective dosage ranges, and demonstrating its potential for developing ART- and cGAS-STING-targeted therapies, ultimately advancing clinical translation.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5329-5342"},"PeriodicalIF":4.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gubi Decoction Ameliorates Porous Cartilage Endplate in an Intervertebral Disc Degeneration Model Mouse Through Inhibition of NF-κB Activity and Pyroptosis.","authors":"Sai Yao, Yanan Li, Hongfeng Ruan, Lianguo Wu, Hanbing Zeng","doi":"10.2147/JIR.S492365","DOIUrl":"https://doi.org/10.2147/JIR.S492365","url":null,"abstract":"<p><strong>Background: </strong>Intervertebral disc (IVD) degeneration (IVDD) is highly prevalent among the elderly population and stands as a leading cause of low back pain. Our prior studies have highlighted the therapeutic potential of Gubi decoction (GBD) in alleviating knee osteoarthritis, however, but the specific mechanism of GBD in treating IVDD is not clear.</p><p><strong>Objective: </strong>To ascertain the clear mechanism of GBD for enhancing its therapeutic efficacy in treating lVDD, through comparison of its effects across different doses of GBD and clinical positive control drugs using a mouse IVDD model.</p><p><strong>Methods: </strong>In this study, 8-week-old male mice were treated with lumbar spine instability (LSI) surgery to construct IVDD model mice. From day 3 post-LSI surgery, mice in the loxoprofen sodium tablets (LST), GBD-L, GBD-M and GBD-H groups were gavage administration with LST (23.1 mg/kg) and GBD (6.1, 12.2 and 24.4 g/kg body weight, respectively) 5 times a week for 4 and 8 weeks separately. After 8 weeks of LSI modeling, the therapeutic efficacy on IVDD was evaluated through changes in lumbar spine function, histopathological morphology, extracellular matrix (ECM) metabolism, nucleus pulposus (NP) cell viability, and cartilage endplate (CEP) cell pyroptosis; at 4 weeks after modeling, the activation of NF-κB signaling was detected.</p><p><strong>Results: </strong>GBD can attenuate the progression of IVDD in mice, resulting in substantially increases disc height index (DHI) and NP matrix, reduced the degree of annulus fibrosus (AF) tear and the formation of cavity in CEP. In parallel, GBD significantly improved the matrix metabolism-related indexes of IVD at 8 weeks after modeling. Mechanically, GBD inhibited the expression of pyroptosis-related indicators NOD-like receptor thermal protein-domain associated protein 3 (NLRP3), cysteinyl aspartate specific-proteinase-1 (CASPASE1), gasdermin D (GSDMD), interleukin-1β (IL-1β) and interleukin-18 (IL-18) in CEP. Furthermore, GBD suppressed nuclear translocation of P65 protein, and decreased the amount of p-I-κB in CEP at 4 weeks after modeling.</p><p><strong>Conclusion: </strong>In summary, GBD can effectively inhibit the activation of NF-κB signaling and pyroptosis of ECP, relieve the porosity of ECP, and then delay the IVDD process. GBD may serve as a potential therapeutic agent for IVDD treatment.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5293-5309"},"PeriodicalIF":4.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12013640/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic Value of Red Blood Cell Width Distribution-to-Platelet Ratio in Patients with Snakebite-Associated Multiple Organ Dysfunction Syndrome: A Retrospective Observational Study.","authors":"Xun Liang, Danlei Xing, Zhiwei Zhuang, Hui Feng, Shiji Li, Xiao Fang, Fei Wang, Le Qiu, Xu-Lin Chen","doi":"10.2147/JIR.S507234","DOIUrl":"https://doi.org/10.2147/JIR.S507234","url":null,"abstract":"<p><strong>Background: </strong>Snakebite-induced organ dysfunction emerging as the primary determinant of morbidity and mortality. This study aims to explore the prognostic value of red blood cell distribution width (RDW) to platelet ratio (RPR) on multi-organ dysfunction (MODS) in patients with snake bites.</p><p><strong>Methods: </strong>A retrospective study included 637 patients with snakebite between 2015 and 2020 from two hospitals in Anhui province, China. Data were collected at two time points: on the 1^st-day and the 5^th-day after treatment. All patients were divided into two groups according to the presence or absence of MODS. <i>T</i>-tests, chi-square tests, and univariate and multivariate logistical analyses were used to identify the prognostic factors for the development of MODS.</p><p><strong>Results: </strong>56 (8.8%) patients developed MODS following snakebite. Logistics analyses indicated that from being bitten at the hospital, the 1^st-day of red blood cell distribution width-to-platelet ratio (RPR) and creatinine (CR) levels, and the 5^th-day of aspartate aminotransferase (AST) were significantly associated with the development of MODS. The sensitivity and the specificity of the 1^st-day RPR were calculated by the received operating characteristic curve (AUC=0.720, 95% CI, 0.642-0.798). The 1^st day RPR=0.110 and the 5^th day RPR=0.085.</p><p><strong>Conclusion: </strong>The study found that the RPR is an independent risk factor for predicting multi-organ dysfunction in patients with snake bites. The 1^st-day RPR >0.110 is prone to be a new independent predictive factor for the development of MODS after snakebite.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5281-5291"},"PeriodicalIF":4.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12013637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time-Varying Effects of Glucocorticoid Treatment in Critically III Patients with Severe Fever with Thrombocytopenia Syndrome: An Inverse Probability of Treatment Weighting Analysis.","authors":"Peng Xia, Yun Liu, Jun Wang, Haopeng Li, Yu Zhai, Baoyan Wang, Hanwen Tong, Weihong Ge, Chenxiao Jiang","doi":"10.2147/JIR.S505421","DOIUrl":"https://doi.org/10.2147/JIR.S505421","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the efficacy of glucocorticoid treatment in critically ill patients with severe fever with thrombocytopenia syndrome (SFTS) and to assess whether glucocorticoid use increases the risk of fungal infections.</p><p><strong>Patients and methods: </strong>A retrospective cohort study was conducted involving confirmed SFTS patients from a tertiary hospital. After applying the Inverse Probability of Treatment Weights (IPTW), multivariable Cox regression and logistic regression analyses were utilized to assess the impact of glucocorticoids on the 28-day mortality rate and the risk of fungal infections. Additionally, landmark analysis and time-varying Cox regression were employed to evaluate the effects of glucocorticoids on mortality across different time intervals.</p><p><strong>Results: </strong>The study included 112 patients with severe SFTS, comprising 67 patients in the glucocorticoid (GC) group and 45 in the non-glucocorticoid (non-GC) group. While glucocorticoid treatment did not significantly alter the overall 28-day mortality in severe SFTS (aHR 0.92, 95% CI 0.44-1.93, P = 0.828), it was associated with a notable reduction in mortality within the first 7 days of hospitalization (aHR 0.35, 95% CI 0.15-0.82, P = 0.016) and an increased mortality risk between days 7 and 28 (aHR 4.92, 95% CI 1.30-18.67, P = 0.019). Furthermore, glucocorticoid use was linked to a significantly higher risk of developing fungal infections (aOR 15.22, 95% CI 4.04-57.38, P < 0.001).</p><p><strong>Conclusion: </strong>The effects of glucocorticoid treatment in severe SFTS patients vary depending on the disease stage, suggesting that the timing of glucocorticoid administration is crucial. Additionally, the increased risk of fungal infections warrants careful consideration when prescribing glucocorticoids in this population.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5311-5327"},"PeriodicalIF":4.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12013642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Psoriatic Efficacies of Psorogrit and Divya-Taila, in Murine Models of Imiquimod and TPA-Induced Psoriasis-Like Inflammation are Driven by Modulation in IL-17RA/IL-23 and IL-8/TNF-α Signaling Axes.","authors":"Acharya Balkrishna, Sonam Sharma, Tapan Dey, Madhulina Maity, Sunil Shukla, Ankita Kumari, Meenu Tomer, Rishabh Dev, Sandeep Sinha, Anurag Varshney","doi":"10.2147/JIR.S505245","DOIUrl":"https://doi.org/10.2147/JIR.S505245","url":null,"abstract":"<p><strong>Aim: </strong>Psoriasis is a chronic inflammatory skin disease that occurs among all age groups, irrespective of gender, and consequently it negatively impacts patient's quality of life. Medicines of herbo-mineral origin are being increasingly used for the mitigation of psoriasis, due to the side effects associated with the available treatment options. Present study characterizes the pharmacological efficacy of Psorogrit (PSO) and Divya-Taila (DT) using in vitro and in vivo assays.</p><p><strong>Methods: </strong>Human keratinocyte (HaCaT) cells stimulated with TNF-α or Imiquimod (IMQ) were used to generate the in vitro models of psoriasis. PSO was further evaluated for modulation of mRNA expression, cytokine levels and NF-κB reporter activity. The in vivo anti-psoriatic activity of the orally given PSO and topically applied DT was assessed in mouse models of IMQ-induced psoriasis-like skin lesions and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema. The animals were randomly allocated to the Normal control, Disease control, Clobetasol, PSO and DT groups. Analysis of ear thickness, ear punch weight, spleen weight, histopathology by hematoxylin and eosin (H&E), and <i>Keratin 17</i> (<i>KRT 17</i>) mRNA expression was measured for evaluation of these herbal formulations. Moreover, the phytochemical composition of PSO and DT was evaluated by UHPLC and GC/MS/MS.</p><p><strong>Results: </strong>Cytosafe concentrations of PSO significantly attenuated IL-8 release as well as mRNA expressions of <i>IL-8</i>, <i>TNF-α</i>, and <i>IL-1β</i> in TNF-α-induced human skin keratinocytes. PSO was observed to decrease the TNF-α-induced NF-κB reporter activity. Additionally, in IMQ-induced HaCaT cells, PSO reduced the release of IL-17RA and mRNA expression of <i>IL-23</i> and <i>IL-17RA</i>. In the in vivo IMQ-induced model, PSO and DT were able to ameliorate the IMQ-induced increase in ear punch weight, relative spleen weight, and histopathological changes in both ear and dorsal back skin. In TPA-induced ear edema model, PSO and DT reduced the increase in ear thickness, ear punch weight, and histopathological lesions. Besides, the phytochemical analysis of PSO and DT revealed the presence of phytometabolites known to have anti-inflammatory activities.</p><p><strong>Conclusion: </strong>The combinatorial use of Psorogrit and Divya-Taila has the potential to ameliorate clinical and pathological manifestations of psoriasis.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5235-5259"},"PeriodicalIF":4.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12013727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro Validation of a Novel Disposable Remover to Remove Activated Leukocytes Generated During Cardiopulmonary Bypass: A Pilot Study.","authors":"Yuling Zheng, Ying Ran, Juan Wu, Ping Yang, Xinyi Liao, Jie Zhang, Wentong Meng, Daming Gou, Li Li, Lei Du, Jing Lin","doi":"10.2147/JIR.S503575","DOIUrl":"https://doi.org/10.2147/JIR.S503575","url":null,"abstract":"<p><strong>Background: </strong>Cardiopulmonary bypass (CPB) is associated with activation of pro-inflammatory cells, which infiltrate tissues and cause injury. Here we explored a novel disposable remover to remove inflammatory leukocytes in order to reduce risk of complications after CPB. This is a substudy within a previously registered clinical trial (NCT05400356) that aims to validate a novel disposable remover to remove activated leukocytes generated during CPB.</p><p><strong>Methods: </strong>The device contains an enhanced biocompatible leukocyte membrane (Chinese patent CN202310822538.X) coated with RGD peptide (Arg-Gly-Asp), which binds to specific polypeptide groups on activated leukocytes, leading to their affinity-based adsorption. The device was integrated into a closed extracorporeal circuit containing a blood reservoir, roller pump, and tubes. Blood from seven patients (150 mL per patient) was driven through the circuit for 10 min at 300 mL/min. Counts of leukocytes and their surface molecules were examined before circulation and after 2.5, 5, 7.5, and 10 min of circulation. The types and morphology of blood cells captured on the filter membrane were also examined.</p><p><strong>Results: </strong>Counts of neutrophils and neutrophils expressing the activation markers CD11b, CD54, CD64 or CD181 decreased rapidly by 36-39% during the first 2.5 min of circulation, after which their counts decreased more slowly. In contrast, counts of monocytes or lymphocytes did not change significantly during circulation. After use, the membrane was still smooth and intact, and it contained substantial numbers of intact activated leukocytes, based on immunostaining against activated cells and scanning electron microscopy. Smears of blood samples before and after circulation showed no significant differences in each leukocyte morphology.</p><p><strong>Conclusion: </strong>This novel disposable remover can preferentially remove activated neutrophils from blood ex vivo, with minimal apparent impact on other leukocytes and blood components.</p><p><strong>Trial registration: </strong>This substudy is part of a prospective cohort study registered at the Clinical Trials Registry (NCT05400356) on 27 May 2022.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5355-5370"},"PeriodicalIF":4.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144026776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of a Risk Prediction Model Based on Inflammatory and Nutritional Composite Indicators for Posthepatectomy Liver Failure Following Radical Resection of Hepatocellular Carcinoma.","authors":"Jingfei Li, Miao Chen, Wei Cai, Dalong Yin","doi":"10.2147/JIR.S515918","DOIUrl":"https://doi.org/10.2147/JIR.S515918","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Purpose: &lt;/strong&gt;A plethora of studies have demonstrated an association between preoperative inflammatory immunonutritional status and the prognosis of patients with hepatocellular carcinoma. Nonetheless, there is a paucity of research examining the predictive value of inflammatory immunonutritional indicators for postoperative liver failure in this patient population. This study seeks to identify independent predictors of post hepatectomy liver failure (PHLF) in patients with hepatocellular carcinoma and to develop a nomogram model.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Patients and methods: &lt;/strong&gt;Clinical data were collected from 760 patients diagnosed with hepatocellular carcinoma who underwent surgical treatment at a hospital in China between January 2020 and January 2024. The dataset was randomly divided into a training set (n=570, 75%) and a validation set (n=190, 25%). To identify independent predictors of PHLF in these patients, univariate analysis and least absolute shrinkage and selection operator (LASSO) regression were employed. Subsequently, a multivariate logistic regression model was developed to construct a predictive model. The predictive performance of the nomogram was evaluated using receiver operating characteristic (ROC) curve analysis, calibration curve assessment, and decision curve analysis (DCA).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;AAPR, ALBI, GAR, LMR, PNI, INR, APTT, and TT are independent factors associated with PHLF in patients with hepatocellular carcinoma. The C indices for the training and validation datasets were 0.691 (95% CI: 0.634-0.747) and 0.680 (95% CI: 0.556-0.804), respectively. The area under the curve (AUC) and calibration curve analyses demonstrated the nomogram's accuracy in predicting PHLF in this patient population. Furthermore, DCA indicated that the model provides a significant clinical net benefit. A comparison was made of the predictive efficacy of the nomogram prediction model and the associated composite liver function score. ROC curves were plotted for the nomogram prediction model, Child-Pugh score and ALBI score, and AUC values were calculated, which were 0.686 (95% CI 0.635-0.737) for the prediction model, 0.558(95% CI 0.512-0.603) for the Child-Pugh score. The AUC for ALBI score was 0.577 (95% CI 0.530-0.624), indicating that this nomogram prediction model was more effective than other scoring systems in predicting the study population in our center. In this study population, the nomogram model demonstrated an AUC of 0.707 (95% CI 0.620-0.794) for Child-Pugh score grade A and 0.572 (95% CI 0.501-0.643) for Child-Pugh score grade B. For tumors with a diameter of less than 5 cm, the AUC was 0.679 (95% CI 0.608-0.749), and for patients with tumors with a diameter of at least 5 cm, the AUC was 0.715 (95% CI 0.643-0.787).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;We have developed an innovative nomogram model designed to predict the incidence of PHLF in patients diagnosed with hepatocellular carcinoma. This nomogr","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5261-5279"},"PeriodicalIF":4.2,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12015730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144014244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}