Journal of Inflammation Research最新文献

{"title":"Identification of Hub Genes for Dexmedetomidine Alleviation of Limb Ischemia-Reperfusion-Induced Lung Injury in Rats by Transcriptomic.","authors":"Kejian Lu, Maoyao Ling, Mei Rao, Haosong Huang, Shucong Liang, Yanxia Wei, Lijuan Bai, Yanjuan Huang, Linghui Pan","doi":"10.2147/JIR.S512536","DOIUrl":"https://doi.org/10.2147/JIR.S512536","url":null,"abstract":"<p><strong>Background: </strong>Limb ischemia-reperfusion (LIR), a prevalent clinical condition, frequently precipitates acute lung injury (ALI). Dexmedetomidine (DEX), a selective alpha2-adrenergic receptor agonist, mitigates LIR-induced ALI. However, its underlying mechanisms remain incompletely elucidated. This study aimed to identify hub genes implicated in DEX-mediated protection against LIR-ALI in rats.</p><p><strong>Methods: </strong>Sprague-Dawley rats were allocated into five groups (n = 3 per group): Sham (femoral artery exposure without occlusion), LIR, LIR + DEX, LIR + Inhibitor, and LIR + DEX + Inhibitor. LIR was induced by clamping the femoral arteries for 3 hours, followed by reperfusion. DEX (50 μg/kg) or Atipamezole (alpha2-receptor inhibitor, 250 μg/kg) was administered prior to ischemia. Lung injury was evaluated <i>via</i> hematoxylin-eosin staining, wet/dry ratio assessment, and quantification of IL-1beta, TNF-alpha, malondialdehyde (MDA), and superoxide dismutase (SOD) levels. RNA sequencing was performed to identify differentially expressed genes (DEGs), followed by functional enrichment analysis, protein-protein interaction (PPI) network construction, and hub gene identification. Gene-gene interaction (GGI) networks were established. Polymerase chain reaction (PCR) and enzyme linked immunosorbent assay (ELISA) validation was conducted.</p><p><strong>Results: </strong>LIR induced severe lung injury and inflammation, both of which were attenuated by DEX pretreatment. RNA sequencing identified 2,302 DEGs1, 471 DEGs2, 340 DEGs3, and 1,407 DEGs4. After intersection and subtraction analyses, 255 DEX-associated DEGs (DEGs-Dex) and 290 inhibitor-associated DEGs (DEGs-In) were identified, with enrichment in Wnt/PI3K-Akt signaling (DEX) and glycerolipid/butanoate metabolism (In). Nine Hub-Dex genes and four Hub-In genes were identified, among which Selp and Tars1 exhibited a strong positive correlation (correlation = 0.55, P < 0.05). Six hub genes (Tars1, Atf4, Ep300, Sphk1, AABR07051376.1, and Mmp9) were validated.</p><p><strong>Conclusion: </strong>Six hub genes associated with DEX-mediated protection against LIR-ALI were identified, providing mechanistic insights and potential therapeutic targets for intervention.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5427-5445"},"PeriodicalIF":4.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12036607/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunoglobulin G N-Glycosylation and Inflammatory Factors: Analysis of Biomarkers for the Diagnosis of Moyamoya Disease.","authors":"Xu Zan, Chao Liu, Xinyue Wang, Shuyu Sun, Zhongchen Li, Wenyu Zhang, Tanggui Sun, Jiheng Hao, Liyong Zhang","doi":"10.2147/JIR.S512707","DOIUrl":"https://doi.org/10.2147/JIR.S512707","url":null,"abstract":"<p><strong>Purpose: </strong>N-glycosylation-modified immunoglobulin G (IgG) is crucial for managing the inflammatory response balance and significantly influences the progression of many inflammatory disorders. IgG N-glycosylation has been demonstrated to correlate with many risk factors for moyamoya disease (MMD), such as hypertension, diabetes, and dyslipidemia. This research aimed to evaluate the diagnostic efficacy of IgG N-glycosylation for MMD.</p><p><strong>Methods: </strong>Ultra-high-performance liquid chromatography (UPLC) was employed to examine the properties of IgG N-glycans in blood samples from 116 patients with MMD and 126 controls, resulting in the quantitative determination of 24 initial glycan peaks (GP). Through the Lasso algorithm and multivariate logistic regression analysis, we constructed a diagnostic model based on initial glycans and related inflammatory factors to distinguish MMD patients from healthy individuals.</p><p><strong>Results: </strong>After adjusting for potential confounding variables, including age, fasting blood glucose (FBG), total cholesterol (TC), high-density lipoprotein (HDL), low-density lipoprotein (LDL), neutrophil count (NEUT), and lymphocyte count (LYM), our study demonstrated significant differences in the characteristics of 6 initial glycans and 16 derived glycans between the MMD cohort and the healthy control group. Based on the above findings, we developed an MMD diagnostic model that combines initial glycans with related inflammatory factors. The curve of receiver operating characteristic (ROC) was utilized to evaluate the model's ability to distinguish MMD patients from healthy subjects. The findings indicated a robust area under the curve (AUC) of 0.963 (95% CI: 0.940, 0.987).</p><p><strong>Conclusion: </strong>This study found that the occurrence and progression of MMD may be associated with decreased levels of sialylation, galactosylation, and fucosylation and increased bisecting GlcNAc. This may be involved in the occurrence of MMD by regulating the balance of inflammation. Therefore, the IgG N-glycosylation is expected to become a potential biomarker for the screening of MMD.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5447-5462"},"PeriodicalIF":4.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12036608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143985302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Levels of IL-6, IL-10, TNF-α, and Vitamin D: Their Correlation with Acute Otitis Media in Short-Stature Pediatric Patients.","authors":"Arif Dermawan, Bejo Ropii, Lina Lasminingrum, Wijana Hasansulama, Budi Setiabudiawan","doi":"10.2147/JIR.S500675","DOIUrl":"https://doi.org/10.2147/JIR.S500675","url":null,"abstract":"<p><strong>Introduction: </strong>Short stature in children is a global health issue often linked to micronutrient deficiencies like vitamin D, potentially impairing immune function. Acute Otitis Media (AOM) is a common complication of upper respiratory infections in children, with inflammatory cytokines such as IL-6, IL-10, and TNF-α playing a crucial role in its pathogenesis. This study aims to explore the correlation between serum levels of these biomarkers and AOM occurrence in short-stature pediatric patients.</p><p><strong>Purpose: </strong>To assess the relationship between serum levels of IL-6, IL-10, TNF-α, and vitamin D and their association with AOM in children with short stature.</p><p><strong>Patients and methods: </strong>A case-control study was conducted with 180 children aged 24-59 months, divided into four groups: short stature with AOM, short stature without AOM, normal stature with AOM, and normal stature without AOM. Serum levels of IL-6, IL-10, TNF-α, and vitamin D were measured using the ELISA method. The data were analyzed using descriptive, bivariate, and multivariate statistical methods.</p><p><strong>Results: </strong>Children with short stature and AOM had the highest median levels of IL-6 (12.15 pg/mL) and TNF-α (162.52 pg/mL), while IL-10 levels were lowest in this group (0.56 pg/mL), indicating a robust inflammatory response associated with AOM. Multivariate analysis revealed that male sex, vitamin D levels ≤18.9 ng/mL, IL-6 levels >9.42 pg/mL, and IL-10 levels ≤0.76 pg/mL were significant risk factors for developing AOM, with respective odds ratios indicating markedly elevated risks.</p><p><strong>Conclusion: </strong>This study underscores the significant role of inflammatory markers and vitamin D deficiency in the development of AOM in short-stature pediatric patients. Regular monitoring and targeted interventions may help prevent AOM in this vulnerable population, warranting future research on the effectiveness of vitamin D supplementation and other therapeutic strategies.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5533-5543"},"PeriodicalIF":4.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12036606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Protective Effect of Bilirubin on MAFLD May Be Mediated by Improving Insulin Re-Sistance and Alleviating Chronic Inflammation.","authors":"Mengying Yang, Jing Liu, Xiaoman Liu, Qianqian Li, Jun Liu, Baogui Wang","doi":"10.2147/JIR.S520257","DOIUrl":"https://doi.org/10.2147/JIR.S520257","url":null,"abstract":"<p><strong>Background: </strong>Bilirubin, as a potent endogenous antioxidant, has demonstrated protective effects in various metabolic and inflammatory diseases. However, the precise role and underlying mechanisms of bilirubin in metabolic-associated fatty liver disease (MAFLD) remain unclear.</p><p><strong>Methods: </strong>This study involved 3000 participants, categorized into non-MAFLD and MAFLD groups. Using weighted multiple linear regression and mediation effect analysis, this study examined the protective impact of total bilirubin (TBIL), direct bilirubin (DBIL), and indirect bilirubin (IBIL) on MAFLD risk. Additionally, potential mediators-inflammation and insulin resistance (IR) through which bilirubin exerts its protective effects were explored.</p><p><strong>Results: </strong>TBIL and DBIL levels in the MAFLD group were significantly lower than those in the non-MAFLD group. Multiple linear regression analysis, adjusted for confounding variables, revealed that compared to the lowest tertile group (TBIL < 14.6), the odds ratios (ORs) for the middle tertile (TBIL 14.6-19.2) and the highest tertile (TBIL ≥ 19.3) groups were 0.735 and 0.615. Similarly, compared to the lowest tertile group (DBIL < 3.4), the ORs for the middle tertile (DBIL 3.4-4.4) and the highest tertile (DBIL ≥ 4.5) groups were 0.613 and 0.367. Mediation analysis revealed significant indirect effects of SIRI, PIV, TyG, TyGBMI, METS-IR, and AIP on the relationship between TBIL, DBIL, and MAFLD risk. Specifically, SIRI mediated 4.07% and 1.55% of the TBIL-MAFLD and DBIL-MAFLD associations, respectively; PIV mediated 9.56% and 4.22%; TyG mediated 69.27% and 81.91%; TyGBMI mediated 100% and 78.34%; METS-IR mediated 100% and 81.41%; and AIP mediated 100% for both TBIL-MAFLD and DBIL-MAFLD associations.</p><p><strong>Conclusion: </strong>Our findings suggest that increased serum levels of TBIL and DBIL are significantly inversely correlated with MAFLD risk, with both serving as independent protective factors against MAFLD occurrence. Further mediation analysis indicates that this protective effect is likely mediated by improvements in IR and the alleviation of systemic chronic inflammation.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5555-5572"},"PeriodicalIF":4.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12036688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urine Neutrophil Elastase: A Novel Predictor of ICU Admission for Patients with COVID-19 Infection.","authors":"Yu Song, Kai Zeng, Li-Kun Zhang, Jian-Nan Zhang, Kai-Li Zhang, Yu Xin, Xin-Ran Wang, Yu-Xin Zhou, Hong-Xu Li, Chang-Song Wang, Kai-Jiang Yu","doi":"10.2147/JIR.S503276","DOIUrl":"https://doi.org/10.2147/JIR.S503276","url":null,"abstract":"<p><strong>Introduction: </strong>We aimed to explore the differences of neutrophil elastase (NE) levels between intensive care unit (ICU) and non-ICU patients with COVID-19 infection, as well as its predictive value for COVID-19 progression.</p><p><strong>Methods: </strong>We enrolled the patients admitted with a primary diagnosis of COVID-19. All patients in ICU were diagnosed with the critical type upon admission. Blood was taken within 24 hours, followed by examination of the blood NE level and urine NE level. Other clinical features were recorded. A logistic regression model was used to predict ICU admission.</p><p><strong>Results: </strong>A total of 83 patients were diagnosed, including 52 non-ICU cases and 31 ICU cases. The ICU group showed significantly elevated levels of Neutrophil%, Cr, D-dimer (DD), Procalcitonin (PCT), and C-reactive protein (CRP). Meanwhile, the CD3-cell, T4-cell, and Lymphocyte% levels were lower in the ICU group. Notably, the blood NE levels were similar between groups, whereas the urine NE level was highly significantly higher in the ICU group vs the non-ICU group. After dimension reduction, we constructed a logistic model (UD) using only two factors: the urine NE level and the blood DD level. The overall accuracy of was 86.1%. The urine NE has a strong efficacy in ICU prediction (AUC = 0.893), and the performance of the UD model was even better (AUC = 0.933).</p><p><strong>Conclusion: </strong>Urine NE level is a useful predictor of COVID-19 progression, particularly in patients requiring ICU care. Urine NE has a significantly positive correlation with neutrophil%, DD, and PCT, as well as a negative correlation with lymphocyte levels.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5545-5553"},"PeriodicalIF":4.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12036589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Experimental Validation of PANoptosis-Related Genes in Idiopathic Pulmonary Fibrosis by Bioinformatics Analysis.","authors":"Dongguang Wang, Yifan Yuan, Xiang Tong, Lian Wang, Jibo Sun, Shijie Zhang, Sitong Liu, Huatian Gan, Hong Fan","doi":"10.2147/JIR.S505229","DOIUrl":"https://doi.org/10.2147/JIR.S505229","url":null,"abstract":"<p><strong>Aim: </strong>To identify the molecular signature of differentially expressed genes (DEGs) associated with PANoptosis in idiopathic pulmonary fibrosis (IPF) and to interpret their immune landscape and cellular distribution characteristics.</p><p><strong>Methods and results: </strong>We acquired two IPF datasets from the Gene Expression Omnibus (GEO) database to identify PANoptosis-related DGEs (PAN-DEGs), initially identifying thirty PAN-DEGs. Utilizing machine learning algorithms, we established a five-gene PANoptosis-related signature comprising IGF1, GPX3, GADD45β, SMAD7, and TIMP3, each demonstrating robust diagnostic performance. The expression of these hub genes was subsequently validated using a third GEO dataset and a bleomycin-induced pulmonary fibrosis model. Immune infiltration analysis revealed a close association of these genes with various immune cells, and single-cell RNA sequencing indicated significant expression changes in diverse pulmonary cell types, particularly endothelial cells and fibroblasts.</p><p><strong>Conclusion: </strong>We identified and validated a PANoptosis-related gene signature in IPF, providing insights into their immune infiltration and potential cellular distribution. Further research is necessary to elucidate the biological functions and mechanisms of these genes in the pathogenesis of IPF.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5499-5517"},"PeriodicalIF":4.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12034268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bergenin Alleviates Myocardial Ischemia/Reperfusion Injury via Regulating SIRT1 Through Ferroptosis.","authors":"Lingmei Jia, Siqi Yang, Jun Yin, Ouhua Feng, Zhigang Wang, Min Jia","doi":"10.2147/JIR.S497618","DOIUrl":"https://doi.org/10.2147/JIR.S497618","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the protective effect of bergenin on myocardial ischemia/reperfusion (I/R) injury and to elucidate its underlying mechanism.</p><p><strong>Methods: </strong>The in vivo model of myocardial I/R injury was established by transient ligation of the left anterior descending coronary artery in Sprague-Dawley rats, which were divided into sham, I/R, I/R+bergenin, and I/R+bergenin+erastin (an agonist of ferroptosis) groups.After the model was established, the rats underwent echocardiography to assess the cardiac function. Hematoxylin and eosin (HE) staining and Masson's trichrome staining were performed to evaluate the cardiac pathological damage. Malondialdehyde (MDA), reactive oxygen species (ROS), glutathione (GSH) and iron levels were measured to determine the ferroptosis level. Western blotting was used to detect the expression of related proteins. Next, H9C2 cells were subjected to oxygen-glucose deprivation/reoxygenation (OGD/R) to mimic the in vitro model of myocardial I/R injury. EX527, a SIRT1 inhibitor, was used to further explore the role of SIRT1 in the myocardial protection of bergenin. In this part of the experiment, H9C2 cells were divided into four groups: control, OGD/R, OGD/R+bergenin, and OGD/R+bergenin+EX527.</p><p><strong>Results: </strong>In vivo experiments, we found that the I/R group showed obvious myocardial pathological damage, oxidative stress and ferroptosis, while the bergenin pretreatment group reversed the above myocardial injury, but this protective effect was inhibited by the ferroptosis inducer erastin. In vitro experiments, compared with the OGD/R group, the bergenin group reduced the oxidative stress level, mitochondrial dysfunction and ferroptosis of H9C2 cells. We found that the protective effect of bergenin on the myocardium was abrogated by EX527. Moreover, Western blotting showed that bergenin activated SIRT1, and increased the phosphorylation of AMPK and the expression level of PGC-1α.</p><p><strong>Conclusion: </strong>Bergenin exerted a protective effect on the myocardium by modulating the ferroptosis process during myocardial I/R injury through the SIRT1/AMPK/PGC-1α pathway.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5519-5531"},"PeriodicalIF":4.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12035748/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sphingolipid Metabolism and Signalling Pathways in Heart Failure: From Molecular Mechanism to Therapeutic Potential.","authors":"Meng Zhao, Rutao Bian, Xuegong Xu, Junpeng Zhang, Li Zhang, Yi Zheng","doi":"10.2147/JIR.S515757","DOIUrl":"https://doi.org/10.2147/JIR.S515757","url":null,"abstract":"<p><p>Sphingolipids are essential components of cell membranes and lipoproteins. They are synthesized de novo in the endoplasmic reticulum and subsequently undergo various enzymatic modifications in different organelles, giving rise to a diverse range of biologically active compounds. These molecules play a critical role in regulating cell growth, senescence, migration, apoptosis, and signaling. In recent years, the sphingolipid metabolic pathway has been recognized as a key factor in heart failure (HF) pathophysiology. Abnormal levels of sphingolipid metabolites, such as ceramide (Cer) and sphingomyelin (SM), contribute to oxidative stress and inflammatory responses, ultimately promoting cardiomyocyte apoptosis. Conversely, sphingosine-1-phosphate (S1P) and ceramide-1-phosphate (C1P) regulate vascular function and influence cardiac remodeling. Additionally, enzymes such as diacylglycerol acyltransferase 1 (DGAT1) and sphingosine-1-phosphate lyase 1 (SGPL1) modulate cardiac lipid metabolism. Given their role in HF progression, monitoring sphingolipid alterations offers potential as valuable biomarkers for assessing disease severity, prognosis, and diagnosis. Given the complexity of sphingolipid metabolism and its involvement in diverse regulatory biological processes, a comprehensive understanding of its roles at both the cellular and organismal levels in physiopathology remains incomplete. Therefore, this review aims to explore the physiological functions, regulatory mechanisms, and therapeutic potential of sphingolipid metabolism. It will summarize the specific molecular mechanisms driving key pathological processes in HF, including ventricular remodeling, myocardial fibrosis, vascular dysfunction, and metabolic disorders. Finally, the review will highlight targeted sphingolipid metabolites as potential therapeutic strategies, offering new insights into HF diagnosis and treatment, with the goal of advancing adjunctive clinical therapies.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5477-5498"},"PeriodicalIF":4.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12034266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CALLY Index as a Predictive Tool for Postoperative Pneumonia in Esophageal Squamous Cell Carcinoma: A Retrospective Cohort Study.","authors":"Zhiyun Xu, Chen Chen, Jianqiang Zhao, Chenglin Li, Bao Zang, Xinkui Xiong","doi":"10.2147/JIR.S517074","DOIUrl":"https://doi.org/10.2147/JIR.S517074","url":null,"abstract":"<p><strong>Background: </strong>Esophageal Squamous Cell Carcinoma poses a significant global health challenge, with postoperative pneumonia being a critical complication affecting recovery and prognosis. Traditional predictive models have proven to be insufficient. This study investigates the CALLY Index as a novel tool for predicting postoperative pneumonia in resectable ESCC patients.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted involving 209 patients undergoing thoraco-laparoscopic McKeown procedure for resectable ESCC from January 2019 to December 2022. Patients with chronic pulmonary diseases or previous malignancies were excluded. Clinical data, including age, gender, tumor stage, preoperative albumin, lymphocyte counts, and CRP levels, were analyzed to calculate the CALLY Index. Univariate and multivariate logistic regression analyses were performed to identify independent predictors of postoperative pneumonia, and receiver operating characteristic curves were used to evaluate the CALLY Index's predictive validity.</p><p><strong>Results: </strong>Among the cohort, 63.8% of patients with low CALLY Index scores developed postoperative pneumonia compared to 12.1% with high scores (p < 0.001). The optimal cutoff for the CALLY Index was determined to be 3.47, achieved sensitivity of 0.721 and specificity of 0.865. In multivariate analyses, the CALLY Index remained a strong predictor of pneumonia (adj. OR: 0.64, 95% CI: 0.51-0.77, p < 0.001). Notably, higher tumor stage and prolonged hospital stays were also associated with an increased risk of pneumonia.</p><p><strong>Conclusion: </strong>The CALLY Index is an effective predictor of postoperative pneumonia in patients with esophageal squamous cell carcinoma, especially when evaluated in conjunction with tumor stage and length of hospital stay. This approach can aid clinicians in conducting early risk assessments and customizing therapeutic strategies, ultimately enhancing patient management and outcomes.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5463-5475"},"PeriodicalIF":4.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12034254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large Chengqi Decoction Improves Sepsis-Related Intestinal Damage by Inhibiting Inflammatory Response Through the HMGB1-TLR4 Signaling Pathway.","authors":"Qiandong Zhu, Zimu Pan, Zhenxing Li, Ren Ye, Yangyang Zhuang, Mei Yang, Weiwei Wang, Jingye Pan, Qiuqi Gao","doi":"10.2147/JIR.S490679","DOIUrl":"https://doi.org/10.2147/JIR.S490679","url":null,"abstract":"<p><strong>Purpose: </strong>This study aimed to evaluate the therapeutic efficacy of Large Chengqi Decoction(LCD) in attenuating sepsis-related intestinal injury by targeting the HMGB1-TLR4 signaling pathway.</p><p><strong>Methods: </strong>Seventy-five Sprague-Dawley (SD) rats were utilized to establish a septic intestinal injury model, randomized into sham operation, model control, and three treatment groups (LCD0.1, LCD1, LCD10). HMGB1, TLR4, IL-6, and MCP-1 levels in intestinal tissues were assessed via ELISA and Western blotting. Histopathological changes were examined using HE staining of ileum sections.</p><p><strong>Results: </strong>Compared to the sham group, the model group showed significant elevation of inflammatory markers, confirming successful model establishment. In the LCD1 group, HMGB1 levels were notably higher at 3 and 5 days, accompanied by consistent TLR4 downregulation. IL-6 levels were significantly reduced at 3 days, and MCP-1 levels were lower compared to the sham group. LCD10 group exhibited decreased HMGB1 levels at 5 days and reduced IL-6 levels at 3 days. Immunohistochemical analysis at 3 days post-modeling indicated that LCD1 group expressions of HMGB1, TLR4, NF-κB, and MCP-1 resembled the sham group and significantly differed from the model group. Both LCD1 and LCD10 groups showed improved ileal damage and reduced edema compared to the model group.</p><p><strong>Conclusion: </strong>LCD effectively mitigates inflammatory responses in septic rats by modulating the HMGB1-TLR4/NF-κB pathway, thereby promoting intestinal repair. Concentrations of 1g/mL and 10g/mL present promising therapeutic strategies for sepsis-related intestinal injury, highlighting the potential of traditional Chinese medicine in sepsis treatment research.</p>","PeriodicalId":16107,"journal":{"name":"Journal of Inflammation Research","volume":"18 ","pages":"5415-5425"},"PeriodicalIF":4.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12034253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}