Drug Testing and Analysis最新文献

筛选
英文 中文
Self-Reported Use of Thyroid Hormones by Athletes at the Olympic Games. 奥运会运动员自我报告的甲状腺激素使用情况。
IF 2.6 3区 医学
Drug Testing and Analysis Pub Date : 2025-07-02 DOI: 10.1002/dta.3923
Mark Stuart, Damien Rhumorbarbe, Audrey Kinahan, Matti L Gild, Roderick Clifton-Bligh, David Handelsman
{"title":"Self-Reported Use of Thyroid Hormones by Athletes at the Olympic Games.","authors":"Mark Stuart, Damien Rhumorbarbe, Audrey Kinahan, Matti L Gild, Roderick Clifton-Bligh, David Handelsman","doi":"10.1002/dta.3923","DOIUrl":"https://doi.org/10.1002/dta.3923","url":null,"abstract":"<p><p>Thyroid hormones (TH) are widely used for treatment of hypothyroidism or thyroid cancer in medical practice, but their use among elite athletes without thyroid disease remains controversial. Despite lacking clear ergogenic benefits, some athletes reportedly use thyroxine (T4) and/or triiodothyronine (T3) with the belief that they enhance performance or facilitate weight management. This study investigates self-reported TH use by athletes at the Tokyo 2020, Beijing 2022, and Paris 2024 Olympic Games, analyzing trends based on sex, age, country, and sport. Across these three events, 1.7% of tested athletes reported TH use, with reported use higher among females and in explosive power sports, which is higher than expected for the youthful Olympic participants studied. However, TH use has declined to 1.3% at Paris 2024 compared to the previous Games (Tokyo 2020: 1.8%; Beijing 2022: 2.7%). Whether this decline is due to a growing awareness of the potential harms and/or lack of performance benefits could not be determined by this study.</p>","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolic Studies of Nandrolone Decanoate in Castrated Horses After Intramuscular Administration. 癸酸诺龙在去势马肌肉内的代谢研究。
IF 2.6 3区 医学
Drug Testing and Analysis Pub Date : 2025-07-01 DOI: 10.1002/dta.3919
Yat-Ming So, Wai Him Kwok, Christina W Y Tang, Celia O L Wong, Terence S M Wan, Emmie N M Ho
{"title":"Metabolic Studies of Nandrolone Decanoate in Castrated Horses After Intramuscular Administration.","authors":"Yat-Ming So, Wai Him Kwok, Christina W Y Tang, Celia O L Wong, Terence S M Wan, Emmie N M Ho","doi":"10.1002/dta.3919","DOIUrl":"https://doi.org/10.1002/dta.3919","url":null,"abstract":"<p><p>This paper describes the studies of the in vitro biotransformation of nandrolone decanoate and its metabolic fate in equine plasma and urine after intramuscular administration to castrated thoroughbred horses. The in vitro metabolic study was performed using homogenised horse liver, and the more prominent in vitro biotransformation pathways were found to include hydrolysis, reduction, oxidation and sulfation, mainly resulting in seven Phase I metabolites and one Phase II metabolite. The administration study of nandrolone decanoate was carried out using three retired thoroughbred geldings, each of which was administered intramuscularly with 800 mg of nandrolone decanoate (Deca-Durabolin) once weekly for three consecutive weeks. Nandrolone decanoate and the majority of the identified in vitro metabolites were detectable in post-administration plasma samples for at least 20 days (last sample collected) after the last administration. Although nandrolone decanoate was not found in any post-administration urine, nandrolone (as a metabolite) and its downstream metabolites can be detected for at least 20 days post-administration (last sample collected). For doping control purpose, nandrolone decanoate itself could be a suitable target analyte in horse plasma for effectively controlling its misuse in equine sports.</p>","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Toward a Cannabis Terroir: Untargeted Metabolomic Profiling of Authentic Samples Using Gas Chromatography-High-Resolution Mass Spectrometry (GC-HRMS) and Liquid Chromatography-High-Resolution Tandem Mass Spectrometry (LC-HRMS/MS). 走向大麻风土:使用气相色谱-高分辨率质谱(GC-HRMS)和液相色谱-高分辨率串联质谱(LC-HRMS/MS)对真实样品进行非靶向代谢组学分析。
IF 2.6 3区 医学
Drug Testing and Analysis Pub Date : 2025-06-30 DOI: 10.1002/dta.3922
Sandra N Poetzsch, Michael Poetzsch, Thomas Kraemer, Andrea E Steuer
{"title":"Toward a Cannabis Terroir: Untargeted Metabolomic Profiling of Authentic Samples Using Gas Chromatography-High-Resolution Mass Spectrometry (GC-HRMS) and Liquid Chromatography-High-Resolution Tandem Mass Spectrometry (LC-HRMS/MS).","authors":"Sandra N Poetzsch, Michael Poetzsch, Thomas Kraemer, Andrea E Steuer","doi":"10.1002/dta.3922","DOIUrl":"https://doi.org/10.1002/dta.3922","url":null,"abstract":"<p><p>Cannabis sativa L. constituents, such as cannabinoids, terpenes, flavonoids, and other secondary metabolites, determine the plant's (medicinal) effects and properties in a complex interplay, a phenomenon known as the entourage effect. However, environmental influences like cultivation method, soil, light, and climate might also influence the plant's chemical composition-and thus its therapeutic profile. Much like in viticulture, the concept of a \"cannabis terroir\" might play an important role in determining the plant's chemical phenotype. The aim of this study was therefore to make these complex properties analytically accessible and develop a comprehensive metabolomics workflow using gas chromatography-high-resolution mass spectrometry (GC-HRMS) and liquid chromatography-high-resolution tandem mass spectrometry (LC-HRMS/MS) in positive and negative ionization mode, applying HILIC and reversed phase chromatography to assess multiple chemical classes. Data processing and statistical analysis were done in MS-DIAL and MetaboAnalyst, respectively. The method was applied to 35 CBD-type cannabis flowers grown under different environmental conditions, and compounds belonging to various chemical classes were successfully detected. Principal component analysis revealed distinct clustering of the samples, and key discriminative features were identified, including cannabinoids, terpenes such as β-caryophyllene and α-humulene, cuticular alkanes (e.g., pentacosane and nonacosane), and polar compounds such as choline and trigonelline. The markers enabled a discrimination of samples not only by chemical phenotype but also by cultivation environment, supporting the emerging concept of a cannabis terroir. In conclusion, this study introduces an analytical framework for the comprehensive chemical profiling of cannabis employing GC-HRMS and LC-HRMS analysis and advanced statistical techniques.</p>","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
SARMs, Metabolic Modulators and Growth Hormone Secretagogues in Suspected Illegal Medicines, Bought as Sport Performance Enhancers: A Retro- and Prospective Study Within the GEON. SARMs,代谢调节剂和生长激素分泌剂涉嫌非法药物,作为运动成绩增强剂购买:在GEON的回顾性和前瞻性研究。
IF 2.6 3区 医学
Drug Testing and Analysis Pub Date : 2025-06-23 DOI: 10.1002/dta.3918
M Mendoza Barrios, E Deconinck, C Vanhee, E K Lamme, I 't Hart-Bakker, P V Syversen, O Bøyum, G Li-Ship, S Young, A Blazewicz, M Poplawska, B Hakkarainen, N Hang Huynh, A Hackl, M J Portela, P Martinho, N Beerbaum, M C Gaudiano, M Raimondo, V Marleau, J Cloutier, J Ollerenshaw, A Hansen, J Mills, M Aha, C Luchte, M Miquel
{"title":"SARMs, Metabolic Modulators and Growth Hormone Secretagogues in Suspected Illegal Medicines, Bought as Sport Performance Enhancers: A Retro- and Prospective Study Within the GEON.","authors":"M Mendoza Barrios, E Deconinck, C Vanhee, E K Lamme, I 't Hart-Bakker, P V Syversen, O Bøyum, G Li-Ship, S Young, A Blazewicz, M Poplawska, B Hakkarainen, N Hang Huynh, A Hackl, M J Portela, P Martinho, N Beerbaum, M C Gaudiano, M Raimondo, V Marleau, J Cloutier, J Ollerenshaw, A Hansen, J Mills, M Aha, C Luchte, M Miquel","doi":"10.1002/dta.3918","DOIUrl":"https://doi.org/10.1002/dta.3918","url":null,"abstract":"<p><p>Although the abuse of muscle-building compounds in elite sports is already known for a long time, these products have become more popular in recreational sport over the past years. Although anabolic steroids are still the most popular ones in this context, the use of other molecules with anabolic properties is on the rise. Three categories of such products are the selective androgen receptor modulators (SARMs), the metabolic modulators and the growth hormone secretagogues (GHS). Based on this trend and the outcomes of a previous market surveillance study in the domain of illegal products (MSSIP), the Falsified Medicines Working Group of the General European Official Medicines Control Laboratories (OMCL) Network (GEON) decided to conduct an MSSIP with focus on SARMs, metabolic modulators and GHS over a period of 5 years. In total 324 samples and 354 results, reported by 14 laboratories in 13 countries, members of the GEON, were taken into account, for which the majority of the samples originated from illegal distribution. Sixty-five percent of the seized products were represented as medicine, though 24% as dietary supplements, which is of concern because here the (recreational) sporter is not aware he is taking an (unapproved) pharmaceutical. Eighteen different molecules, within the scope of the study, were reported with as top 5: ibutamoren, ligandrol, ostarine, cardarine and andarine. From the limited quantitative data reported, it can be assumed that the majority of the samples contain active doses and some are even overdosed, so health risks for the consumers cannot be neglected.</p>","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Monitoring Community Use of Benzodiazepines Through Wastewater-Based Epidemiology. 通过基于废水的流行病学监测社区苯二氮卓类药物的使用。
IF 2.6 3区 医学
Drug Testing and Analysis Pub Date : 2025-06-18 DOI: 10.1002/dta.3915
Maarten Quireyns, Tim Boogaerts, Natan Van Wichelen, Diāna Vanaga-Arāja, Olesia Rudminienė, Ruxanda Iliescu, Milica Georgescu, Sofie Schaerlaekens, Naomi De Roeck, Peter Delputte, Adrian Covaci, Alexander L N van Nuijs
{"title":"Monitoring Community Use of Benzodiazepines Through Wastewater-Based Epidemiology.","authors":"Maarten Quireyns, Tim Boogaerts, Natan Van Wichelen, Diāna Vanaga-Arāja, Olesia Rudminienė, Ruxanda Iliescu, Milica Georgescu, Sofie Schaerlaekens, Naomi De Roeck, Peter Delputte, Adrian Covaci, Alexander L N van Nuijs","doi":"10.1002/dta.3915","DOIUrl":"https://doi.org/10.1002/dta.3915","url":null,"abstract":"<p><p>Benzodiazepines and related z-drugs (BZDs) are widely used pharmaceuticals but require careful monitoring due to limited efficacy, tolerance development and the risk of dependence. Wastewater-based epidemiology (WBE) is a valuable approach to gather population-wide information on consumption patterns through analysis of influent wastewater. This study examines how overlapping metabolic pathways within the BZD drug class can lead to misinterpretation if not carefully considered. An analytical method is developed and validated for 26 biomarkers of prescription BZDs in influent wastewater using solid-phase extraction and liquid chromatography-tandem mass spectrometry and applied to influent wastewater samples for the first time in Belgium (13 locations), Latvia (1), Lithuania (3) and Romania (1). The countries were chosen for their differing prescription BZD market registration, allowing testing of different hypotheses. To aid in complex data interpretation, this study presents a structured categorisation of BZDs into direct biomarkers, which directly reflect consumption, and downstream biomarkers, which are influenced by multiple BZDs, and further discusses the implication this has on the interpretation. Spatial differences were noted among direct biomarkers such as higher consumption of zolpidem in Belgium and zopiclone in Riga (LVA). Downstream biomarkers had higher population-normalised mass loads but cannot be interpreted in isolation, for example, lorazepam (ranging 4-25 mg/day/1000 inhabitants over all locations), lormetazepam (8-30), oxazepam (10-50) and temazepam (1-12). These findings highlight challenges in interpreting BZD consumption trends and emphasise the need of distinguishing different biomarker classes to ensure accurate and comparable results across studies needed to guide informed decision-making.</p>","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of an Optimized Extraction Method to Recover Drug Material From Used Test Strips for Comprehensive Drug Checking. 综合药检用试纸中药物提取方法的优化研究。
IF 2.6 3区 医学
Drug Testing and Analysis Pub Date : 2025-06-18 DOI: 10.1002/dta.3911
Meghan G Appley, Elise M Pyfrom, Rae A Elkasabany, Rick Rousch, Edward Sisco
{"title":"Development of an Optimized Extraction Method to Recover Drug Material From Used Test Strips for Comprehensive Drug Checking.","authors":"Meghan G Appley, Elise M Pyfrom, Rae A Elkasabany, Rick Rousch, Edward Sisco","doi":"10.1002/dta.3911","DOIUrl":"https://doi.org/10.1002/dta.3911","url":null,"abstract":"<p><p>Drug-checking programs use point-of-need testing (e.g., test strips) and laboratory-based analysis to rapidly identify emerging drug threats, but each has limitations. Test strips are quick but compound or class specific, whereas laboratory testing can identify more compounds but have lengthy turnaround times. To address these limitations, it was proposed that compounds could be extracted from used test strips for additional analyses allowing for rapid on-site information followed by comprehensive laboratory results. The method development process involved four parts: determining the optimal extraction approach, assessing the feasibility of performing direct analysis in real-time mass spectrometry (DART-MS) analysis on extracts, determining the limits of detection (LODs) for a range of analytes, and evaluating the method using used test strips submitted by harm reduction sites. The optimized method consisted of extracting analytes of interest from a cut test strip using 0.5-mL methanol while vortexing for 10 s. DART-MS successfully identified the compounds of interests, and the test strip chemical background was identified. LODs were found to be as low as a mass fraction of 0.005 in a mixture. For the samples submitted by harm reduction sites, concordance between extracts and test strip results was 96%, and the agreement in compound identification between used test strip extracts and authentic drug collection samples was approximately 80% regardless of test strip type and preparation. This work shows that additional analyses of extracted test strips can provide a low-barrier way for high-quality testing that can be used to increase data on the drug landscape.</p>","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of Methyltestosterone and Testosterone Propionate in Dried Blood Spots After Transdermal Application. 经皮应用后干血斑中甲基睾酮和丙酸睾酮含量分析。
IF 2.6 3区 医学
Drug Testing and Analysis Pub Date : 2025-06-05 DOI: 10.1002/dta.3914
Asami Miyamoto, Masato Okano, Masanori Ota, Mitsuhiko Sato
{"title":"Analysis of Methyltestosterone and Testosterone Propionate in Dried Blood Spots After Transdermal Application.","authors":"Asami Miyamoto, Masato Okano, Masanori Ota, Mitsuhiko Sato","doi":"10.1002/dta.3914","DOIUrl":"https://doi.org/10.1002/dta.3914","url":null,"abstract":"<p><p>In the past few years, doping tests have been performed with dried blood spots (DBS) as test samples; this sampling method is minimally invasive and requires minimal storage space. Steroid esters are stable in dried blood, allowing the direct analysis of testosterone esters. In Japan, the cream-based formulation containing 17α-methyltestosterone (MeT) and testosterone propionate (TP) is available as an over-the-counter drug for hair growth. Here, we investigated whether the transdermal uptake of this formulation could be detected using the existing method for steroid ester analysis in DBS. A method that was previously validated to detect and identify TP could also detect MeT. We developed a high throughput liquid chromatography-tandem mass spectrometry method to identify MeT in DBS. The limits of detection and identification of MeT were 0.1 ng/mL and 0.6 ng/mL, respectively. Five male human subjects received the cream-based formulation of MeT and TP transdermally. We then collected blood samples by finger pricking and made DBS on cellulose paper. Using this method, MeT was detected and identified up to 97 h after the final application. TP was also detected up to 97 h and identified up to 49 h after the final application. The developed method provides a direct confirmation of the presence of the drug. Moreover, using cream preparations could lead to contamination from MeT and TP remaining on the fingertip skin during fingertip puncture sampling.</p>","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of Transdermal Application of Testosterone to Racehorses by Analysis of Urine and Plasma. 通过尿液和血浆分析检测经皮给药赛马的睾酮。
IF 2.6 3区 医学
Drug Testing and Analysis Pub Date : 2025-06-03 DOI: 10.1002/dta.3905
Marjaana Viljanto, Charlotte Cutler, Jocelyn Habershon-Butcher, Pamela Hincks, James Scarth
{"title":"Detection of Transdermal Application of Testosterone to Racehorses by Analysis of Urine and Plasma.","authors":"Marjaana Viljanto, Charlotte Cutler, Jocelyn Habershon-Butcher, Pamela Hincks, James Scarth","doi":"10.1002/dta.3905","DOIUrl":"https://doi.org/10.1002/dta.3905","url":null,"abstract":"<p><p>The use of testosterone in racehorses is predominantly monitored using international urine and plasma concentration-based thresholds and complementary steroid ratios. To date, there has been no published pharmacokinetic study on transdermally applied testosterone products in horses and whether their use could result in adverse analytical findings. Therefore, quantitative analysis of testosterone and epitestosterone in urine and testosterone in plasma samples was performed following a pilot multi-dose transdermal Testogel administration (1 mg/kg once a day for 7 days on clipped skin) to one gelding and one mare. The peak concentrations (C<sub>max</sub>) of free testosterone were 1060 and 1800 pg/mL in gelding and mare plasma, respectively. Testosterone concentrations exceeded the international plasma threshold of 100 pg/mL consistently for up to 4 h post-administration, after which detection above the threshold was sporadic up to 127 h. In urine, C<sub>max</sub> of free and conjugated (sulfate and glucuronide) testosterone were 700 and 323 ng/mL in gelding and mare urine, respectively. In the gelding, testosterone concentrations exceeded the international urine threshold of 20 ng/mL consistently for up to 47 h post-administration, but sporadically up to 143 h. In all samples, testosterone: epitestosterone ratios were greater than 5, another requirement for adverse analytical findings in geldings. In the mare, testosterone concentrations exceeded the urine threshold of 55 ng/mL consistently for up to 71 h post-administration, but sporadically up to 167 h. Therefore, these limited results for one gelding and one mare demonstrate that doping control following transdermal applications of testosterone to racehorses is possible using existing approaches.</p>","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A "Pheraplex" Capsule Labeled as Desoxymethyltestosterone From the Market Turned Out to Be 17,17-Dimethyl-18-nor-5α-androst-13-en-3β-ol. 市场上一种标记为脱氧甲基睾酮的“Pheraplex”胶囊原来是17,17-二甲基-18-no -5α-androst-13-en-3β-醇。
IF 2.6 3区 医学
Drug Testing and Analysis Pub Date : 2025-05-23 DOI: 10.1002/dta.3912
Sisi Zhu, Shan Wang, Xin Liu, Lisi Zhang
{"title":"A \"Pheraplex\" Capsule Labeled as Desoxymethyltestosterone From the Market Turned Out to Be 17,17-Dimethyl-18-nor-5α-androst-13-en-3β-ol.","authors":"Sisi Zhu, Shan Wang, Xin Liu, Lisi Zhang","doi":"10.1002/dta.3912","DOIUrl":"https://doi.org/10.1002/dta.3912","url":null,"abstract":"<p><p>In an effort to conduct a desoxymethyltestosterone (DMT) administration study to replenish excretion urine inventory of Beijing Anti-Doping Laboratory for quality assurance purpose, a product labeled as \"Pheraplex\" was purchased from the internet. The product's label indicated that each capsule contained 10 mg of 17α-methyl-etioallocholan-2-ene-17β-ol (DMT), along with medicinal corn starch and gelatin. To verify the product's contents, gas chromatography tandem quadrupole mass spectrometry (GC-MS/MS) was employed to analyze the active ingredient and compare it with the reference materials of DMT. Surprisingly, the results revealed that the product did not contain DMT or any other steroids monitored in the initial testing procedure of our laboratory. Subsequently, nuclear magnetic resonance was utilized to identify the compound's structure, which was determined to be 17,17-dimethyl-18-nor-5α-androst-13-en-3β-ol. This compound was referred to as M10 of 17α-methyltestosterone in a literature. This finding highlights the potential discrepancies between product labeling and actual contents in the supplement market, which deserves attention from the anti-doping community.</p>","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Diagnostic Sensitivity and Specificity of a New Automatized Assay for Indirect Synthetic Urine Identification. 评价一种新的自动化间接合成尿液鉴定方法的诊断敏感性和特异性。
IF 2.6 3区 医学
Drug Testing and Analysis Pub Date : 2025-05-22 DOI: 10.1002/dta.3908
Laura Franke, Christian Fuczik, Dirk K Wissenbach
{"title":"Evaluation of Diagnostic Sensitivity and Specificity of a New Automatized Assay for Indirect Synthetic Urine Identification.","authors":"Laura Franke, Christian Fuczik, Dirk K Wissenbach","doi":"10.1002/dta.3908","DOIUrl":"https://doi.org/10.1002/dta.3908","url":null,"abstract":"<p><p>Synthetic urine mimicking normal urine is difficult detectable by standard specimen validity testing. In this study, the VDx True Urine LD Test and True Urine SD Test, analysing 'long duration' (LD) and 'short duration' (SD) markers, were evaluated for their ability to detect synthetic urine. Two synthetic urines from the US market and 1188 real-life urine specimens were analysed for LD markers, SD markers, uric acid and temperature. Forty-seven specimens (set 1) showed uric acid and/or temperature suspicious results and were analysed by LC-MS/MS for 10 endogenous biomolecules. Diagnostic sensitivities and specificities were calculated for LD and SD markers based on uric acid and LC-MS/MS results of set 1. The false-positive rate of the SD marker was further evaluated with uric acid results of the other 1141 specimens (set 2). Additionally, direct synthetic urine markers were analysed by LC-(HR)MS. Uric acid and LC-MS/MS could identify synthetic urine. In one US synthetic urine, low concentrations of uric acid were found. Specimens of the Austrian/German region were reliably classified congruently by both methods. The LD and the SD markers detected both synthetic urines. For set 1, the LD had 100% sensitivity and 88.9% specificity, and the SD had 84.2% sensitivity and 44.4% specificity for authentic/synthetic specimens. In set 2, the SD marker specificity depended on the upper limit of the acceptance criteria. In one US synthetic urine, carbendazim was identified. Overall, several tests should be applied to confirm authentic or synthetic urine. Especially, simultaneous analysis of indirect and direct synthetic urine markers should be considered.</p>","PeriodicalId":160,"journal":{"name":"Drug Testing and Analysis","volume":" ","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信