合成代谢雄激素类固醇和类固醇酯的检测——干燥血斑采集装置与尿样的比较。

IF 2.7 3区 医学 Q2 BIOCHEMICAL RESEARCH METHODS
Tina Suominen, Jeanette von Walden, Laura Harju, Anton Pohanka, Jenny Schulze, Mikael Lehtihet, Lena Ekström
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引用次数: 0

摘要

干血斑(DBS)已成为一种有希望的补充,在某些情况下,可替代尿液进行合成代谢雄激素(AAS)检测,具有微创、简化储存和运输等优点。本研究评估了两种DBS收集设备——基于纤维素的Capitainer-B50和基于聚合物的tasso - m20,并将结果与传统的尿液分析进行了比较。招募了10名自我报告的AAS使用者,并提供了匹配的尿液和DBS样本。两种DBS装置之间的一致性很高,尽管capitainb - b50的检出率略高,这可能是由于更高的样品体积(50 μL vs. 17.5 μL)提高了分析物回收率,并且背景噪声更低。值得注意的是,DBS能够检测所有10名参与者的睾酮使用情况,而尿液检测遗漏了两例天然低尿睾酮/表睾酮(T/E)比率(最有可能是UGT2B17 del/del携带者)。此外,在DBS中也可以区分处方和非法使用睾酮酯,但在尿液检测中无法区分,而在DBS中检测到的诺龙在低浓度时受到限制。研究结果支持DBS作为一种灵敏实用的AAS检测工具,并在检测处方睾酮治疗个体和UGT2B17缺失携带者的睾酮酯掺杂方面提供了关键优势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection of Anabolic Androgenic Steroids and Steroid Esters-Comparing Dried Blood Spots Collection Devices and Urine Samples.

Dried blood spots (DBS) have emerged as a promising complement, and in some settings, an alternative, to urine for anabolic androgenic steroid (AAS) testing, offering advantages such as minimal invasiveness, simplified storage, and transportation. This study evaluated two DBS collection devices-cellulose-based Capitainer-B50 and polymer-based Tasso-M20-and compared results with traditional urine analysis. Ten self-reported AAS users were recruited and provided matched urine and DBS samples. High agreement between the two DBS devices was observed, although Capitainer-B50 showed a slightly greater detection rate, likely due to a higher sample volume (50 μL vs. 17.5 μL) improved analyte recovery, and lower background noise. Notably, DBS enabled detection of testosterone use in all 10 participants, while urine testing missed two cases with naturally low urinary testosterone/epitestosterone (T/E) ratios (most likely UGT2B17 del/del carriers). Moreover, the differentiation between prescribed and illicit use of testosterone esters was also possible in DBS, but not in urine testing, while nandrolone detection in DBS was limited at low concentrations. The findings support DBS as a sensitive and practical tool for AAS detection and provide critical advantages in detecting doping with testosterone esters in individuals with prescribed testosterone therapy and in UGT2B17 deletion carriers.

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来源期刊
Drug Testing and Analysis
Drug Testing and Analysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL
CiteScore
5.90
自引率
24.10%
发文量
191
审稿时长
2.3 months
期刊介绍: As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances. In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds). Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.
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