Journal of Histochemistry & Cytochemistry最新文献_第4页

Akkermansia muciniphila Selectively Reshapes Small Intestinal Cell Populations. 嗜粘杆菌选择性地重塑小肠细胞群。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-01 Epub Date: 2026-02-16 DOI: 10.1369/00221554261417734

Rachel Edens-Valentine, Kristen A Engevik, Melinda A Engevik, Amy C Engevik

{"title":"Akkermansia muciniphila Selectively Reshapes Small Intestinal Cell Populations.","authors":"Rachel Edens-Valentine, Kristen A Engevik, Melinda A Engevik, Amy C Engevik","doi":"10.1369/00221554261417734","DOIUrl":"10.1369/00221554261417734","url":null,"abstract":"The gastrointestinal tract harbors a dynamic microbial ecosystem that interfaces with the intestinal epithelium. Among this community, Akkermansia muciniphila, a mucin-degrading microbe, has garnered attention for its impact on gut health. While well studied in the colon, its influence on the small intestine remains underexplored. To examine direct effects of A. muciniphila, we used gnotobiotic mice. Germ-free mice were inoculated with Brain Heart Infusion (BHI) media or 10⁹ viable A. muciniphila in BHI. After 21 days, small intestinal tissue was collected. Fluorescence in situ hybridization confirmed A. muciniphila colonization. Immunofluorescence staining revealed increased epithelial cell proliferation, unchanged goblet cell numbers, but altered mucus composition with reduced fucose residues. Tuft cell numbers and group 2 innate lymphoid cells (ILC2s) were also elevated in A. muciniphila-colonized mice. Analysis of conditioned media and the A. muciniphila genome identified succinate production, a metabolite known to expand tuft cells. Thus, A. muciniphila alone is sufficient to increase tuft cells in the small intestine, potentially via succinate signaling. These findings reveal a novel role for A. muciniphila in regulating gastrointestinal homeostasis.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"215-233"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12913043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146202035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting CCNB1 Repressed Cartilage Degradation Induced by Inflammation Through NF-κB Pathway in Osteoarthritis. 靶向CCNB1通过NF-κB途径抑制炎症诱导的骨关节炎软骨降解。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-01 Epub Date: 2026-02-23 DOI: 10.1369/00221554251410643

Hua Li, Yuxue Qiao, Mengshuang Ding, Yirong Liu

{"title":"Targeting CCNB1 Repressed Cartilage Degradation Induced by Inflammation Through NF-κB Pathway in Osteoarthritis.","authors":"Hua Li, Yuxue Qiao, Mengshuang Ding, Yirong Liu","doi":"10.1369/00221554251410643","DOIUrl":"10.1369/00221554251410643","url":null,"abstract":"Inflammation contributes to osteoarthritis, and cyclin B1 (CCNB1) dysregulation is implicated. Understanding its role and regulation is crucial for developing targeted therapies against this degenerative disease. Mice chondrocytes were acquired from C57BL/6 wild-type mice and treated with interleukin (IL)-1β for inflammation induction, followed by assays for cell viability, apoptosis, inflammatory mediators, cartilage markers, and nuclear factor kappa B (NF-κB) pathway. CCNB1 was knocked down or upregulated in chondrocytes, respectively. The modified Hulth method was used to establish osteoarthritis model. The knee joint was visualized using micro-computed tomography, and histopathologic evaluation was carried out by immunohistochemistry staining, Safranin O/fast green, and hematoxylin and eosin staining for cartilage degradation markers. CCNB1 knockdown inhibited IL-1β-caused decrease in cell viability and increase in apoptosis of chondrocytes. Inflammatory mediators in IL-1β-treated chondrocytes were decreased after CCNB1 knockdown. CCNB1 knockdown reduced the expression of MMP-13 and ADAMTS-5, while elevated collagen II and aggrecan accumulation, alongside with NF-κB inactivation, in chondrocytes administered with IL-1β. Targeting inhibition of CCNB1 reduced the production of inflammation regulators, decreased cartilage degradation, and blocked NF-κB pathway activation. Targeting CCNB1 may serve as a potential therapeutic strategy for osteoarthritis by reducing inflammation, protecting cartilage, and modulating the NF-κB pathway.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"235-248"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12932133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147275699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lamina Propria Collagen Architecture in Interstitial Cystitis/Bladder Pain Syndrome. 间质性膀胱炎/膀胱疼痛综合征的固有层胶原结构。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-01 Epub Date: 2026-01-17 DOI: 10.1369/00221554251410640

Hannah Ruetten, Raymond Xu, Harrison Martin, Dylan T Wolff, Wencheng Li, Kaylee Ferrara, L McKenna Huse, Robert J Evans, Gopal Badlani, Stephen J Walker

{"title":"Lamina Propria Collagen Architecture in Interstitial Cystitis/Bladder Pain Syndrome.","authors":"Hannah Ruetten, Raymond Xu, Harrison Martin, Dylan T Wolff, Wencheng Li, Kaylee Ferrara, L McKenna Huse, Robert J Evans, Gopal Badlani, Stephen J Walker","doi":"10.1369/00221554251410640","DOIUrl":"10.1369/00221554251410640","url":null,"abstract":"Interstitial cystitis/bladder pain syndrome (IC/BPS) is a heterogeneous condition of uncertain etiology. We assessed bladder collagen characteristics in phenotypically characterized IC/BPS patient subgroups that may influence pathophysiology. Forty-four females (30 IC/BPS; 14 non-IC/BPS) were included. Patients were divided into groups based on Hunner lesions (HL; N=14 or non-HL; N=16) and anesthetic bladder capacity (BC) (BC≤500 cc; low BC; N=17 or BC>500 cc; non-low BC; N=13). Bladder biopsy tissue slides were stained with hematoxylin and eosin or picrosirius red and semi-quantitatively analyzed by a pathologist. CT-FIRE software was used to quantitatively assess lamina propria collagen. All patients with IC/BPS had lower collagen fiber density independent of subgroup (p<0.0001-0.0038) compared to controls. HL had more peri-muscular collagen accumulation (p=0.0061), more acute inflammation (p=0.0364), more severe chronic inflammation (p=0.0088), and narrower collagen fibers than non-HL and controls. Non-low-BC patients had lower collagen density (p=0.0075) and straighter collagen fibers (p=0.0127) than low BC. Low-BC patients had narrower collagen fibers than control (p=0.0096). IC/BPS, regardless of subgroup, is associated with a bladder lamina propria with diminished collagen density. HL, non-low-BC, and low-BC subgroups have unique collagen characteristics. These findings suggest a collagen fiber destruction and redistribution process, which differs by subgroup, and may contribute to pathophysiology of IC/BPS.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"129-142"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12812059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145989531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Expression of Histamine H₃ Receptor In Healthy, Preneoplastic, and Tumoral Pancreatic Tissue: A Pilot Study. 组胺H3受体在健康、肿瘤前和肿瘤胰腺组织中的差异表达：一项初步研究

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-01 Epub Date: 2026-01-01 DOI: 10.1369/00221554251410292

Daniela Speisky, Melisa B Nicoud, Alejandro Iotti, Karina Formoso, Paolo Lauretta, María Teresa García de Dávila, Vanina A Medina

{"title":"Differential Expression of Histamine H3 Receptor In Healthy, Preneoplastic, and Tumoral Pancreatic Tissue: A Pilot Study.","authors":"Daniela Speisky, Melisa B Nicoud, Alejandro Iotti, Karina Formoso, Paolo Lauretta, María Teresa García de Dávila, Vanina A Medina","doi":"10.1369/00221554251410292","DOIUrl":"10.1369/00221554251410292","url":null,"abstract":"Pancreatic ductal adenocarcinoma (PDAC) is one of the leading causes of cancer-related death worldwide, with a mortality rate almost equal to its incidence, highlighting the urgent need for novel biomarkers and therapeutic targets. This pilot study aims to investigate the potential value of histamine H3 receptor (H3R) as a prognostic biomarker for this lethal disease. We analyzed the H3R expression in PDAC using the RNA sequencing data set from The Cancer Genome Atlas (Pan-Cancer Atlas). In addition, H3R protein levels were evaluated by immunohistochemistry in 27 PDAC samples and compared with adjacent preneoplastic pancreatic tissue of the same patient, and with 10 non-related healthy pancreatic tissues. This preliminary study shows that the H3R is barely expressed in healthy tissue. Interestingly, H3R was detected in 96% of PDAC samples, and its expression in tumoral tissue was significantly higher when compared with its expression in preneoplastic tissue, and it was associated with a better prognosis in terms of overall survival. Present findings suggest that H3R may serve as a potential prognostic biomarker in PDAC. Future research aimed at elucidating the role of H3R in PDAC biology and its prognostic value in larger patient cohorts is warranted.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"181-191"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12759016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145889322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes. 联合受激发射耗尽显微镜与荧光寿命成像显微镜研究肾小球足细胞中TIE2蛋白。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-01 Epub Date: 2026-01-21 DOI: 10.1369/00221554251413643

Iulia-Aneta Dénes, Irina A Okkelman, Herlinde De Keersmaecker, Pieter Cornillie, Ruslan I Dmitriev, Ward De Spiegelaere

{"title":"Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes.","authors":"Iulia-Aneta Dénes, Irina A Okkelman, Herlinde De Keersmaecker, Pieter Cornillie, Ruslan I Dmitriev, Ward De Spiegelaere","doi":"10.1369/00221554251413643","DOIUrl":"10.1369/00221554251413643","url":null,"abstract":"The TIE2 transmembrane receptor protein, known for its role in vascular stability and blood vessel remodeling, has primarily been studied in endothelial cells. This receptor has also been found on several non-endothelial cell types including podocytes, although its presence on podocytes remains a matter of debate. Conventional immunofluorescence approaches applied to membrane proteins are often challenged by the strong tissue autofluorescence spanning green and red parts of the electromagnetic spectrum, sample thickness, and the antibody specificity. Here, we used stimulated emission depletion (STED) microscopy, a super-resolution microscopy method, to detect the TIE2 protein in complex biological tissue with a resolution of less than 50 nm. To further confirm the presence of TIE2 on podocytes and to investigate its localization, we used fluorescence lifetime imaging microscopy (FLIM) to more effectively distinguish between nonspecific autofluorescence and specific emission in cleared and antibody-labeled tissue samples. By correlating these two techniques, we mapped the subcellular localization of TIE2 on mouse podocytes and confirmed its presence on the cell surface facing the Bowman's space, albeit at lower expression levels. This study highlights the potential complementary power of STED and FLIM methods and provides additional evidence that the TIE2 receptor is present on podocytes.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"143-161"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12823351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146010745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Brain Tissue Preservation for Nucleic Acid Stability. 核酸稳定性的脑组织保存优化。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-01-01 Epub Date: 2026-01-05 DOI: 10.1369/00221554251400421

Mario Novelli, Caine C Smith, Dhiraj Maskey, Julia Stevens, Marina Gimeno, Neil Horadagoda, Greg T Sutherland

{"title":"Optimization of Brain Tissue Preservation for Nucleic Acid Stability.","authors":"Mario Novelli, Caine C Smith, Dhiraj Maskey, Julia Stevens, Marina Gimeno, Neil Horadagoda, Greg T Sutherland","doi":"10.1369/00221554251400421","DOIUrl":"10.1369/00221554251400421","url":null,"abstract":"Postmortem human brain tissue is an important resource for brain research. Spatial transcriptomics is a novel technology that utilizes formalin-fixed, paraffin-embedded (FFPE) or cryosections, with the former having good cytoarchitecture but poor RNA quality and vice versa for frozen tissue. Sheep brains (n=16) were used to test various protocols that simulate the conditions around the preparation and dissemination of human postmortem FFPE and frozen brain tissue to optimize for future spatial transcriptomic work. FFPE and frozen tissues were investigated for RNA quality, while hematoxylin and eosin-stained cryosections were analyzed by quantifying tissue voids as a proxy for cytoarchitectural integrity. Postmortem interval reduced the RNA integrity number equivalent (RINe) of frozen tissue from 7.2 (24 hr) to 4.8 (168 hr). In FFPE tissue, the percentage of RNA fragments greater than 200 nucleotides (DV200) values ranged from 18.9% to 69.01%, with the highest values observed in samples fixed for less than 24 hr. Pretreatment with liquid nitrogen before -80°C storage resulted in the lowest voids (11.6%) in cryosections, but cryoprotectants had little effect. These findings provide researchers with guidelines for tissue preparation in spatial transcriptomics. However, freezing protocols require further refinement to approach the cytoarchitecture of FFPE tissue.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"101-117"},"PeriodicalIF":1.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12774817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Tetraspanin 4 Relative to Therapy-induced Senescence Markers in Breast Cancer in Response to Neoadjuvant Chemotherapy. 乳腺癌对新辅助化疗的反应中与治疗诱导的衰老标志物相关的Tetraspanin 4的表达

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-01-01 Epub Date: 2025-11-20 DOI: 10.1369/00221554251391226

Tareq Saleh, Sofian Al Shboul, Kholoud Friehat, Nebras Melhem, Alia Al Mohtaseb, Sura B AlOmari, Mohammad Matalka, Nisreen Abu Shahin, Shrouq Daromar, Ahmad Alhesa, Mohammad El-Sadoni, Randa G Naffa, Moureq R Alotaibi, Jack Brydon, Ted Hupp, Jakub Faktor, Kenneth Weke, Sachin Kote, AbdelKader Battah

{"title":"Expression of Tetraspanin 4 Relative to Therapy-induced Senescence Markers in Breast Cancer in Response to Neoadjuvant Chemotherapy.","authors":"Tareq Saleh, Sofian Al Shboul, Kholoud Friehat, Nebras Melhem, Alia Al Mohtaseb, Sura B AlOmari, Mohammad Matalka, Nisreen Abu Shahin, Shrouq Daromar, Ahmad Alhesa, Mohammad El-Sadoni, Randa G Naffa, Moureq R Alotaibi, Jack Brydon, Ted Hupp, Jakub Faktor, Kenneth Weke, Sachin Kote, AbdelKader Battah","doi":"10.1369/00221554251391226","DOIUrl":"10.1369/00221554251391226","url":null,"abstract":"Therapy-induced senescence (TIS) is a component of breast cancer (BC) treatment. Tetraspanins have emerging roles in cancer biology. Tetraspanin 4 (TSPAN4 [NAG2]) has been implicated in tumor progression, however, its association with TIS remains unexplored. We investigated TSPAN4 expression in BC samples from patients who received neoadjuvant chemotherapy (NAC) and its association with TIS markers. Thirty-eight paired pre- and post-NAC BC samples were analyzed using immunohistochemistry (IHC) staining for TSPAN4 and TIS-associated biomarkers (Lamin B1 and Ki67). Pairwise analysis of senescence-related gene expression (LMNB1, MKI67, CDKN1A, ATM, IGFBP7, MMP2, CXCL14, and CCL5) was performed in an independent geneset of 68 paired pre- and post-NAC BC patient samples. NAC reduced the expression of senescence-associated proliferation markers Ki67 and Lamin B1 in BC samples, with 84% and 76% of patients showing decreased expression, respectively (p<0.001). Senescence-associated gene expression analysis revealed consistent upregulation of CDKN1A, ATM, IGFBP7, MMP2, CXCL14, and CCL5 post-NAC (p<0.001), while LMNB1 and MKI67 were significantly downregulated (p<0.0001 and p=0.007, respectively). A subset (15/38; 39%) of samples demonstrated upregulation of the TSPAN4 expression post-NAC (p<0.01). NAG2 was upregulated in 54/68 patients post-NAC (p<0.00001) and its expression correlated positively with senescence-associated genes. An association between TSPAN4 and TIS post-NAC was identified.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"19-37"},"PeriodicalIF":1.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12638236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145563889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological Visualization of Thrombi During Catheter-Directed Fibrinolytic Therapy for Pulmonary Thromboembolism. 导管定向溶栓治疗肺血栓栓塞时血栓形态可视化。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-01-01 Epub Date: 2025-11-25 DOI: 10.1369/00221554251386923

Hirotaka Noda, Chikao Yutani, Satoru Takahashi, Mitsuhiko Takewa, Nobuzo Iwa, Manabu Kobayashi, Sei Komatsu, Kazuhisa Kodama, Hirofumi Yamamoto

{"title":"Morphological Visualization of Thrombi During Catheter-Directed Fibrinolytic Therapy for Pulmonary Thromboembolism.","authors":"Hirotaka Noda, Chikao Yutani, Satoru Takahashi, Mitsuhiko Takewa, Nobuzo Iwa, Manabu Kobayashi, Sei Komatsu, Kazuhisa Kodama, Hirofumi Yamamoto","doi":"10.1369/00221554251386923","DOIUrl":"10.1369/00221554251386923","url":null,"abstract":"Catheter-directed thrombolysis for acute pulmonary thromboembolism (PTE) is associated with a reduction in mortality, although it also carries an increased risk of bleeding. This study aimed to visualize the extent of fibrinolysis within pulmonary artery thrombi using rare specimens obtained via non-obstructive general angioscopy (NOGA). Under direct visualization with NOGA, blood samples were collected from the site of pulmonary artery thrombi and analyzed histologically and immunohistochemically. Fibrinolysis was assessed using the fibrin network index (FNI). The FNI was significantly higher in the Monteplase-treated group than in the untreated group, clearly visualizing the enhanced fibrinolysis induced by Monteplase. In a case where Monteplase was administered in advance for the treatment of deep vein thrombosis (DVT), a marked increase in D-dimer levels was observed; however, the FNI remained low. This suggests that the thrombus may have partially dissolved at the DVT stage, limiting the efficacy of Monteplase by the time the embolus reached the pulmonary artery. The presence of neutrophil extracellular traps (NETs) in association with fibrin was confirmed by immunohistochemical analysis. The influence of NETs in the progression from DVT to PTE was suggested.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"9-17"},"PeriodicalIF":1.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12657208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145604503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytochemical Characterization for Macrophages Engulf Cholesterol Crystals and Myeloperoxidase in Neutrophil Extracellular Traps (NETs) Sampled From Disrupted Aortic Plaques. 巨噬细胞吞噬胆固醇晶体和骨髓过氧化物酶的细胞化学特征在中性粒细胞细胞外陷阱（NETs）中取自主动脉斑块。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-01-01 Epub Date: 2025-12-31 DOI: 10.1369/00221554251392653

Nobuzo Iwa, Hirotaka Noda, Chikao Yutani, Sei Komatsu, Satoru Takahashi, Mituhiko Takewa, Tomoki Ohara, Kazuhisa Kodama

{"title":"Cytochemical Characterization for Macrophages Engulf Cholesterol Crystals and Myeloperoxidase in Neutrophil Extracellular Traps (NETs) Sampled From Disrupted Aortic Plaques.","authors":"Nobuzo Iwa, Hirotaka Noda, Chikao Yutani, Sei Komatsu, Satoru Takahashi, Mituhiko Takewa, Tomoki Ohara, Kazuhisa Kodama","doi":"10.1369/00221554251392653","DOIUrl":"10.1369/00221554251392653","url":null,"abstract":"This study aimed to develop simple and cytochemical detection methods for identifying macrophages that engulf cholesterol crystals (CCs) and for visualizing the granule enzyme myeloperoxidase (MPO) in putative neutrophil extracellular traps (NETs). Specimens were collected from blood-derived debris using filter paper and non-obstructive general angioscopy (NOGA), and analyzed using various staining techniques. Cytochemical staining methods included supra-vital Sternheimer staining, nonspecific esterase staining, and immunocytochemistry, which effectively detected macrophages actively phagocytosing CCs. MPO was visualized using the diaminobenzidine (DAB) method, which demonstrated MPO-positive NETs adhering to fibrin cores, as well as released and dispersed MPO granules. The diameter of MPO-positive granules ranged from 0.1668 to 0.6502 µm. These findings suggest that simple, rapid, and accessible cytochemical techniques are useful for elucidating the pathophysiology of atherosclerotic lesions, particularly in the context of innate immune responses.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"65-75"},"PeriodicalIF":1.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12756049/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145863002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Looking Back to Move Forward: Tannic Acid in TEM of Parasitic Protozoa. 回顾前瞻：寄生原生动物透射电镜中的单宁酸。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-01-01 Epub Date: 2025-11-03 DOI: 10.1369/00221554251388501

Sharmila Ortiz, Wanderley de Souza, Marlene Benchimol

{"title":"Looking Back to Move Forward: Tannic Acid in TEM of Parasitic Protozoa.","authors":"Sharmila Ortiz, Wanderley de Souza, Marlene Benchimol","doi":"10.1369/00221554251388501","DOIUrl":"10.1369/00221554251388501","url":null,"abstract":"Transmission electron microscopy (TEM) is a key tool for the ultrastructural analysis of biological samples; however, it requires optimized fixation and contrast enhancement methods to achieve accurate results. Here, we evaluated the use of tannic acid as a mordant during primary fixation for TEM processing of Giardia intestinalis and Trichomonas vaginalis. When combined with osmium tetroxide (OsO4) post-fixation, tannic acid significantly improved in-block contrast in plasma membranes, organelle boundaries, and cytoskeletal elements, while preserving structural integrity. It increased electron density without introducing artifacts and, in some cases, allowed the omission of lead citrate staining, simplifying the protocol and reducing exposure to toxic agents. Even in the absence of OsO4, samples processed with tannic acid retained sufficient contrast to visualize basal bodies, axonemes, and other cytoskeletal filaments. Moreover, tannic acid enhanced the visualization of poorly characterized structures in the transition zone. We also demonstrate its successful use as a post-staining agent, replacing uranyl acetate for ultrathin sections while maintaining high image quality. These findings support tannic acid as a safe, cost-effective, and efficient alternative to traditional contrast agents, particularly under biosafety constraints, and contribute to the improvement of TEM protocols for studying protozoan morphology and cell biology.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"39-51"},"PeriodicalIF":1.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12583016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145431519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0