Journal of Histochemistry & Cytochemistry最新文献

Detection of the RNA-Editing Enzyme ADARB1 in Primary Auditory Neurons and Anti-ADARB1 Immunofluorescence as a Means for Automated Counting. 原代听觉神经元中rna编辑酶ADARB1的检测及抗ADARB1免疫荧光自动计数方法

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-10-18 DOI: 10.1369/00221554261490630

Garner C Fincher, Punam Thapa, Steven C Gressett, Bradley J Walters

{"title":"Detection of the RNA-Editing Enzyme ADARB1 in Primary Auditory Neurons and Anti-ADARB1 Immunofluorescence as a Means for Automated Counting.","authors":"Garner C Fincher, Punam Thapa, Steven C Gressett, Bradley J Walters","doi":"10.1369/00221554261490630","DOIUrl":"https://doi.org/10.1369/00221554261490630","url":null,"abstract":"Spiral ganglion neurons (SGNs), the primary auditory afferents in the inner ear, are vulnerable to degeneration from neuropathies, concussion, and aging, among other causes. Accurate quantification of SGNs is critical for assessing degeneration and therapeutic interventions, but manual counting is arduous due to dense cellular crowding and unreliable nuclear markers. Because SGNs receive input from GluA2-containing AMPA receptors, the transcripts for which must be RNA-edited for calcium impermeability, we hypothesized that SGNs express high levels of the RNA-editing enzyme ADARB1. Here, we confirm enriched expression of Adarb1 transcripts in SGNs and show that anti-ADARB1 antibodies robustly label the nuclei of type I and type II SGNs from young and aged mice, adult macaques, and adult humans. Neuronal versus glial specificity was confirmed using antibodies against neurofilament heavy chain (NFH), human antigen D (HuD), GATA binding protein 3 (GATA3), and SRY-box 2 (SOX2). A blinded investigator manually counted mouse SGNs via NFH, and these were compared to automated counts of ADARB1-positive nuclei using the analyze particles function in ImageJ. A concordance correlation coefficient and Bland-Altman analysis demonstrated strong agreement between the manual and automated counts. Together, these results suggest broad utility of ADARB1 immunolabeling for automated counts of SGNs across species.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261490630"},"PeriodicalIF":1.5,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlled Extension of the Tumor Promotion Period Enhances Progression of GSTP-Positive Hepatocellular Lesions in a Rat Model of Hepatocarcinogenesis. 在大鼠肝癌发生模型中，肿瘤促进期的可控延长促进了gstp阳性肝细胞病变的进展。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-10-08 DOI: 10.1369/00221554261479389

Carla G Comanzo, Juan P Parody, Florencia Buiatti Fagalde, Marina C Vera, Lucía Oviedo Bustos, Nicolás F Palma, Alejo M Capiglioni, Lautaro Hesse, Gerardo B Pisani, Anabela C Ferretti, María C Carrillo, María P Ceballos, María de L Alvarez, Ariel D Quiroga

{"title":"Controlled Extension of the Tumor Promotion Period Enhances Progression of GSTP-Positive Hepatocellular Lesions in a Rat Model of Hepatocarcinogenesis.","authors":"Carla G Comanzo, Juan P Parody, Florencia Buiatti Fagalde, Marina C Vera, Lucía Oviedo Bustos, Nicolás F Palma, Alejo M Capiglioni, Lautaro Hesse, Gerardo B Pisani, Anabela C Ferretti, María C Carrillo, María P Ceballos, María de L Alvarez, Ariel D Quiroga","doi":"10.1369/00221554261479389","DOIUrl":"10.1369/00221554261479389","url":null,"abstract":"Histochemical approaches are critical for detecting early, clinically silent stages of hepatocarcinogenesis; however, the widely used diethylnitrosamine/2-acetylaminofluorene initiation-promotion (IP) model is time-constrained, limiting evaluation of lesion progression beyond the preneoplastic stage. This study established and validated an extended IP protocol to enhance histochemical detection and stage-resolved analysis of preneoplastic liver lesions while assessing the effects of intermittent fasting (IF) during promotion. Male Wistar rats underwent either the standard IP protocol or an extended version (IPx), in which promotion was prolonged by 3 weeks. IF was implemented during promotion in both models. Preneoplastic lesions were identified by immunohistochemical detection of rat placental glutathione S-transferase P (rGST-P), complemented by histological and molecular analyses. Promotion extension markedly increased the liver area occupied by rGST-P-positive altered hepatic foci and induced pronounced histoarchitectural alterations, improving assessment of advanced preneoplastic stages without progression to overt carcinoma. IF reduced lesion burden and partially preserved liver architecture, particularly in the extended model, and was associated with changes in markers of apoptosis, proliferation, inflammation, and metabolic regulation. These findings validate promotion extension as a methodological refinement of the IP model, enabling detailed analysis of advanced preneoplastic stages and effective evaluation of interventions that modulate early hepatocarcinogenic progression.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261479389"},"PeriodicalIF":1.5,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13652977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary Culture and Characterization of Postnatal Retinal Precursor Cells and Their Potential for Repair of the Damaged Ganglion Layer. 出生后视网膜前体细胞的原代培养和表征及其修复受损神经节层的潜力。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-10-02 DOI: 10.1369/00221554261478977

Neilanjan Maitra, Gowripriya Chidambaranathan, Seema Sen, Madhumita P Ghosh

{"title":"Primary Culture and Characterization of Postnatal Retinal Precursor Cells and Their Potential for Repair of the Damaged Ganglion Layer.","authors":"Neilanjan Maitra, Gowripriya Chidambaranathan, Seema Sen, Madhumita P Ghosh","doi":"10.1369/00221554261478977","DOIUrl":"10.1369/00221554261478977","url":null,"abstract":"Retinal precursor cells (RPCs) derived from embryonic, mesenchymal, induced pluripotent, and neonatal sources have demonstrated significant potential in cell replacement therapies for retinal degeneration. Early postnatal RPCs (PND0-PND8) can integrate into the adult diseased retina; however, their limited survival reduces long-term therapeutic efficacy. In contrast, RPCs from later neonatal stages (>PND15), being more lineage-committed, may yield improved regenerative outcomes, particularly for Müller glia and photoreceptors. In this study, we evaluated RPCs isolated from PND12-PND15 Wistar rat pups obtained from age-matched breeding pairs to assess their pluripotency and regenerative capacity in vivo. A modified protocol incorporating EDTA in nuclease-free buffers was standardized for retinal dissection, processing, and incubation, followed by culture in neurobasal medium supplemented with B27 and FGF2. Undifferentiated neurospheres expressing stem cell markers Nanog and Sox2 were dissociated at 96 hr and transplanted into an adult N-methyl-d-aspartate-induced retinal degeneration model. Compared with early postnatal RPCs (PND2-PND4), which formed smaller, slow-growing neurospheres (2.58 ± 0.15 µm) with rapid differentiation, PND12-PND15 RPCs generated larger, stable neurospheres (4.5 ± 0.18 µm) within 48 hr. Pilot data indicate enhanced potential of these cells in promoting retinal ganglion layer repair.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261478977"},"PeriodicalIF":1.5,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13646189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular Matrix Alterations and the Antifibrotic Role of Mycophenolate Mofetil in a Model of Peyronie's Disease. 细胞外基质改变和霉酚酸酯在Peyronie病模型中的抗纤维化作用

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-09-07 DOI: 10.1369/00221554261484218

Thiago da Silveira Antoniassi, Thalles Fernando Rocha Ruiz, Vitor Grigio, Luiz Roberto Falleiros-Junior, Simone Jacovaci Colleta, Luiz Cesar Fava Spessoto, Sebastião Roberto Taboga, Fernando Nestor Facio-Junior

{"title":"Extracellular Matrix Alterations and the Antifibrotic Role of Mycophenolate Mofetil in a Model of Peyronie's Disease.","authors":"Thiago da Silveira Antoniassi, Thalles Fernando Rocha Ruiz, Vitor Grigio, Luiz Roberto Falleiros-Junior, Simone Jacovaci Colleta, Luiz Cesar Fava Spessoto, Sebastião Roberto Taboga, Fernando Nestor Facio-Junior","doi":"10.1369/00221554261484218","DOIUrl":"10.1369/00221554261484218","url":null,"abstract":"Peyronie's disease (PD) is a fibrotic disorder of the tunica albuginea marked by extracellular matrix (ECM) remodeling, leading to penile curvature and erectile dysfunction. This study aimed to evaluate ECM alterations in a rat model of PD induced by transforming growth factor beta and to assess the potential antifibrotic effects of mycophenolate mofetil (MMF). Thirty male Wistar rats were divided into control, SHAM, PD (7 and 30 days), and PD+MMF-treated (7 and 30 days) groups (n=5/group). Histological and immunohistochemical analyses were performed in the tunica albuginea. PD induction resulted in an imbalance between total collagen and reticulin fibers, a hallmark of early fibrosis, and increased proteoglycan deposition and elastic fiber disorganization. These changes were more pronounced at 30 days post-induction. MMF treatment for 30 days significantly reduced collagen ratio, restored collagen organization, and normalized the distribution of elastic fibers. Proteoglycan levels, elevated in untreated PD animals, were also decreased after 30 days of MMF therapy. In addition, MMF modulated matrix metalloproteinase (MMP) expressions, notably enhancing MMP-2 and MMP-3 levels, and altered the balance of fibroblasts and myofibroblasts in a time-dependent manner. These findings suggest that MMF has antifibrotic effects and may be a promising therapeutic strategy for PD, particularly in the early stages of fibrosis:°.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261484218"},"PeriodicalIF":1.5,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13553973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148906320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteoglycans and Biomineralization of Bone and Dentin. 蛋白聚糖与骨和牙本质的生物矿化。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-09-02 DOI: 10.1369/00221554261478974

Victor E Arana-Chavez, Vivian Bradaschia-Correa, Isabella Z Guiati, Leticia S Castro-Filice

{"title":"Proteoglycans and Biomineralization of Bone and Dentin.","authors":"Victor E Arana-Chavez, Vivian Bradaschia-Correa, Isabella Z Guiati, Leticia S Castro-Filice","doi":"10.1369/00221554261478974","DOIUrl":"10.1369/00221554261478974","url":null,"abstract":"Although many advances have been made during the last six decades, the controlled processes by which vertebrate hard tissues acquire, organize, and maintain their mineral component remain only partially understood. Mineralized tissues can be classified into two categories based on their developmental origin. Enamel is the only hard tissue of epithelial origin; all other hard tissues are of connective tissue origin and therefore possess a collagen-rich extracellular matrix as their main organic component. The cells responsible for the formation of collagen-based hard tissues regulate the synthesis and degradation of non-collagenous components, among which proteoglycans (PGs) play a key role in biomineralization. This review focuses on the mineralization process of bone and dentin, highlighting the highly coordinated sequential events leading to the acquisition of the mineral phase. Initially, the forming cells release numerous small spherical membrane-limited bodies known as matrix vesicles (MVs), which constitute the nanocompartments necessary for the onset of mineral nucleation (vesicular stage). At this stage, extracellular matrix PGs bind to the MV membrane and subsequently to calcium ions, thereby allowing the accumulation of high concentrations of calcium around the MVs. Following PG degradation, calcium ions enter the MVs through annexin channels, leading to the nucleation and growth of the first mineral crystals. Subsequently, cells orchestrate the conditions required for mineral propagation into the surrounding collagenous matrix (fibrillar stage). During this phase, PGs and other non-collagenous matrix components play a crucial role in promoting mineralization progression as well as in limiting and interrupting the process.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261478974"},"PeriodicalIF":1.5,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13538284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148874280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neutrophil Extracellular Traps Formation Adhering to Cholesterol Crystals Modulates Inflammatory Cytokines Release and Promotes Their Disruption: Insights In Situ Touch-imprints Method From Non-obstructive General Angioscopy. 粘附在胆固醇晶体上的中性粒细胞胞外陷阱的形成调节炎症细胞因子的释放并促进它们的破坏：来自非阻塞性普通血管镜检查的原位触摸印迹方法的见解。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-09-01 Epub Date: 2026-08-05 DOI: 10.1369/00221554261473330

Chikao Yutani, Nobuzo Iwa, Hirotaka Noda, Manabu Kobayashi, Satoru Takahashi, Mitsuhiko Takewa, Sei Komatsu, Tomoki Ohara, Kazuhisa Kodama

{"title":"Neutrophil Extracellular Traps Formation Adhering to Cholesterol Crystals Modulates Inflammatory Cytokines Release and Promotes Their Disruption: Insights In Situ Touch-imprints Method From Non-obstructive General Angioscopy.","authors":"Chikao Yutani, Nobuzo Iwa, Hirotaka Noda, Manabu Kobayashi, Satoru Takahashi, Mitsuhiko Takewa, Sei Komatsu, Tomoki Ohara, Kazuhisa Kodama","doi":"10.1369/00221554261473330","DOIUrl":"10.1369/00221554261473330","url":null,"abstract":"Both macrophages and neutrophils are recognized as innate immune cells; the former engulf cholesterol crystals (CCs) while secreting NLRP3, which also promote inflammatory responses and drive the latter into atherosclerotic plaques. Moreover, recent studies have implied a critical role of NLRP3 in neutrophil recruitment into atherosclerotic lesions as neutrophil extracellular traps (NETs), which have not been visualized in vivo adhering on CCs so far. We have observed that neutrophils simultaneously attracted by NLRP3 cause NETs formation in situ from spontaneously ruptured atherosclerotic plaques by using touch-imprints method. This study aimed to confirm by imaging that NETs react to CCs, release NLRP3-related inflammatory factor, and degenerate on the surfaces that might result in breakages. These findings raise the possibility that NET-forming neutrophils not only respond to CCs but may also contribute to their structural modification or fragmentation. Such interactions may represent a previously underappreciated bidirectional relationship between CCs and NETs in human atherothrombosis.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"581-591"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13442907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148673649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seeing, Interpreting, and Trusting Images in the Age of Artificial Intelligence. 人工智能时代的视觉、解读和信任图像。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-09-01 Epub Date: 2026-06-08 DOI: 10.1369/00221554261454582

Katia Cortese, Patrizio Castagnola

{"title":"Seeing, Interpreting, and Trusting Images in the Age of Artificial Intelligence.","authors":"Katia Cortese, Patrizio Castagnola","doi":"10.1369/00221554261454582","DOIUrl":"10.1369/00221554261454582","url":null,"abstract":"Artificial intelligence (AI) is transforming how cell biologists generate, analyze, and interpret visual data. Automated pipelines and large-scale image analysis increase throughput and reproducibility, yet they also modify how visual evidence is validated and trusted. This Perspective examines the conceptual implications of this shift, arguing that the integration of AI into imaging demands rather than replaces human morphological expertise. We distinguish between three conceptually distinct phenomena: (1) automation of acquisition, (2) AI-driven interpretation, and (3) the emergence of synthetic images generated without experimental basis. Through examples ranging from classical morphological misinterpretations to the rise of generative models, we show how both human and algorithmic systems can distort meaning when interpretation is detached from context. While AI can match or surpass human precision, metavisual competence remains essential to distinguish signals from artifacts. The current challenge is not technological but scientific: to ensure that computational power and human insight co-evolve toward reliable visual knowledge. We call for a renewed education in critical visual literacy, formal recognition of imaging specialists, and transparent standards of image provenance, such as the publication of raw instrument metadata. Preserving interpretative competence is crucial for the integrity of visual evidence in biomedical science.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"525-534"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13249602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148204847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial Proteomic Profiling, a Novel Method for Detecting Diagnostic and Prognostic Proteins in Pediatric Sarcoma. 空间蛋白质组学分析，一种检测儿童肉瘤诊断和预后蛋白的新方法。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-09-01 Epub Date: 2026-07-08 DOI: 10.1369/00221554261455962

Abderrahman Ouban, Amani Alkofide, Antonello Podda, Awatif Eid Alanazi, Nader Ashraf, Tarek Ziad Arabi, Belal Nedal Sabbah, Katherine Elston, Vijay Baichwal, Erin Rudzinski, Eleanor Y Chen, Nya D Nelson, Catherine Albert, Atif A Ahmed

{"title":"Spatial Proteomic Profiling, a Novel Method for Detecting Diagnostic and Prognostic Proteins in Pediatric Sarcoma.","authors":"Abderrahman Ouban, Amani Alkofide, Antonello Podda, Awatif Eid Alanazi, Nader Ashraf, Tarek Ziad Arabi, Belal Nedal Sabbah, Katherine Elston, Vijay Baichwal, Erin Rudzinski, Eleanor Y Chen, Nya D Nelson, Catherine Albert, Atif A Ahmed","doi":"10.1369/00221554261455962","DOIUrl":"10.1369/00221554261455962","url":null,"abstract":"Pediatric sarcomas comprise approximately 10% of all childhood solid malignancies and are characterized by distinct genetic and proteomic alterations that have potential diagnostic, prognostic, and therapeutic significance. We have utilized digital spatial profiling (DSP) to identify protein expression in pediatric Ewing sarcoma (ES), Osteosarcoma (OS), Alveolar rhabdomyosarcoma (ARMS), and Embryonal rhabdomyosarcoma (ERMS), in association with clinical outcomes. Formalin-fixed, paraffin-embedded sections from a tissue microarray block containing eight ES, eight OS, five ARMS, and three ERMS cases were subjected to proteomic DSP on a GeoMx NanoString platform yielding information on expression of 580 proteins. Proteins related to epigenetic regulation, signaling pathways, and mesenchymal differentiation were broadly expressed across all tumor types. Tumor-specific protein profiles were defined based on highly expressed proteins. Differentially expressed proteins include Cyclin D1 in ES, S100A4 in OS and IKKi/IKKe in ARMS and ERMS. Immunohistochemical validation confirmed variable expression of H3K27me3 across the tumors, and elevated expression of Cyclin D1 in ES and S100 in OS. These findings underscore the utility of DSP as a high-resolution proteomic tool for the identification of clinically relevant biomarkers in pediatric sarcomas. The results provide a foundation for further investigation of candidate proteins with potential diagnostic, prognostic, and therapeutic applications.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"551-562"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13350073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148411858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ortholog-specific Spatial Expression of Vanins in the Respiratory Tract of Horses. 马呼吸道Vanins的同源特异性空间表达。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-09-01 Epub Date: 2026-08-31 DOI: 10.1369/00221554261474565

Katharina Landmann, Florian Bartenschlager, David Meierhofer, Andreas Spree, Caroline Pisch, Annette Zeyner, Christiane L Schnabel, Lars Mundhenk

{"title":"Ortholog-specific Spatial Expression of Vanins in the Respiratory Tract of Horses.","authors":"Katharina Landmann, Florian Bartenschlager, David Meierhofer, Andreas Spree, Caroline Pisch, Annette Zeyner, Christiane L Schnabel, Lars Mundhenk","doi":"10.1369/00221554261474565","DOIUrl":"10.1369/00221554261474565","url":null,"abstract":"Equine asthma (EA) is the most prevalent chronic respiratory disease in horses, but molecules thought to be relevant in its pathogenesis are not yet fully understood. Recently, increased Vanin-1 (VNN1) levels were found in bronchoalveolar lavage fluid from horses with severe EA. Human VNN1 is known as a pantetheinase, which is associated with respiratory diseases such as asthma, but the equine vanins (eVNNs) are uncharacterized. We identified the domain architecture of eVNN in silico and analyzed its tissue expression in healthy horses using RT-PCR (n=5) and in situ hybridization (n=3). Recombinant eVNNs were expressed in HEK293 cells to assess cellular transport and glycosylation via Endo H and PNGase F treatment. Three putative functional eVNNs were identified on chromosome 10, each containing canonical Nitrilase superfamily domains. All genes showed a broad tissue expression, but distinct airway localization: eVNN1 and eVNN2 were predominantly expressed in respiratory epithelium, whereas eVNN3 was localized in submucosal glands. eVNN1 and eVNN3 were secreted, while eVNN2 remained cell associated. Pantetheinase activity of recombinant eVNN1 was confirmed by mass spectrometry. The three eVNNs appear to cover different functional niches in equine airways, enabling future research into their putative role in EA.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"563-580"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13530036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148864133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and Spatial Localization of Glycosaminoglycan Types in Cartilage From Different Anatomical Locations. 不同解剖部位软骨中糖胺聚糖类型的表达和空间定位。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-09-01 Epub Date: 2026-07-03 DOI: 10.1369/00221554261460112

Manula S B Rathnayake, Reimer V Wolff, Nicole Kops, Karyna Kulakova, Gerjo J V M van Osch, Brooke Farrugia, Kathryn S Stok

{"title":"Expression and Spatial Localization of Glycosaminoglycan Types in Cartilage From Different Anatomical Locations.","authors":"Manula S B Rathnayake, Reimer V Wolff, Nicole Kops, Karyna Kulakova, Gerjo J V M van Osch, Brooke Farrugia, Kathryn S Stok","doi":"10.1369/00221554261460112","DOIUrl":"10.1369/00221554261460112","url":null,"abstract":"Sulphated glycosaminoglycan (sGAG) is a major component of cartilage and known to be involved in cell signaling processes, but little is known about expression and localization in different cartilage types. Using common histochemical staining methods to assess regional sGAG distribution in bovine articular, meniscal, auricular, and nasal cartilage, different staining patterns were found for alcian blue, Safranin O and thionine. Immunohistochemistry to identify expression and location of different epitopes of chondroitin sulfate (C-0-S, C-4-S and C-6-S), dermatan sulfate, keratan sulfate, and heparan sulfate, also demonstrated regional differences which could not explain the differences in histochemical staining. Chondroitin sulfate and keratan sulfate were expressed in all cartilage types investigated, however keratan sulfate was not expressed in the superficial zone of articular cartilage. Dermatan sulfate was only present in tensile load-bearing zones of articular and meniscal cartilage, and in the perichondrium of auricular and nasal cartilage. Despite being hyaline, nasal cartilage and articular cartilage showed dissimilar sGAG expressions. In conclusion, sGAG localization in different types of cartilage is distinctly different, suggesting diverse structural and mechanical functions governed by GAGs. These differences are important for our understanding of the role of sGAG in cartilage function and in cartilage tissue engineering strategies.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"535-550"},"PeriodicalIF":1.5,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13331793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148376009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0