Journal of Histochemistry & Cytochemistry最新文献

From Manual to Macro: A Reproducible Fiji Workflow for Semi-automated Collagen Fibril Diameter Quantification in Transmission Electron Microscopy. 从手动到宏：可复制的斐济工作流程半自动胶原纤维直径定量在透射电子显微镜。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-05-05 DOI: 10.1369/00221554261440588

Apoorva Bhandari, Nobuyo Mizuno, Sara F Tufa, Lynn Y Sakai, Douglas R Keene, Sherene Shalhub

{"title":"From Manual to Macro: A Reproducible Fiji Workflow for Semi-automated Collagen Fibril Diameter Quantification in Transmission Electron Microscopy.","authors":"Apoorva Bhandari, Nobuyo Mizuno, Sara F Tufa, Lynn Y Sakai, Douglas R Keene, Sherene Shalhub","doi":"10.1369/00221554261440588","DOIUrl":"10.1369/00221554261440588","url":null,"abstract":"Quantitative assessment of collagen fibril diameter is essential for understanding ultrastructural changes in aging, connective tissue disorders, and extracellular matrix remodeling. Although transmission electron microscopy (TEM) is widely used for this purpose, existing methods for fibril measurement are predominantly manual, operator-dependent, and prone to inconsistent reporting. To address this, we developed a semi-automated Fiji/ImageJ (IJ1) macro that standardizes fibril diameter measurements from two-dimensional TEMs. The macro uses a wand-based region-of-interest (ROI) detection strategy with integrated geometric validation. It calculates multiple metrics, including area-equivalent diameters, ellipse-derived major and minor axes, shape consistency indices, and spatial localization across the image field. Built-in quality assurance thresholds exclude oblique or irregular profiles, ensuring accurate identification of true fibril cross-sections. Real-time visual overlays support live validation and user feedback during analysis. We detail the implementation, analytical workflow, and validation approach, provide practical guidance for reproducible use across operators and datasets, and show proof-of-concept utility for analysis of collagen fibrils in vascular Ehlers-Danlos syndrome (VEDS) subject dermal samples. This open-source, extensible tool enhances standardization and reproducibility in collagen fibril morphometry for ultrastructural research.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261440588"},"PeriodicalIF":1.5,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13149348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ADAMTS1 Is Required for Ventral Abdominal Wall Closure. 腹腹壁闭合需要ADAMTS1。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-04-28 DOI: 10.1369/00221554261438169

Gabriel Opoku, Kentaro Ikemura, Omer F Hatipoglu, Takashi Ohtsuki, Ikumi Sato, Hibiki Taniguchi, Shino Sakamoto, Farhana Hasib, Shogo Watanabe, Timothy J Mead, Suneel S Apte, Satoshi Hirohata

{"title":"ADAMTS1 Is Required for Ventral Abdominal Wall Closure.","authors":"Gabriel Opoku, Kentaro Ikemura, Omer F Hatipoglu, Takashi Ohtsuki, Ikumi Sato, Hibiki Taniguchi, Shino Sakamoto, Farhana Hasib, Shogo Watanabe, Timothy J Mead, Suneel S Apte, Satoshi Hirohata","doi":"10.1369/00221554261438169","DOIUrl":"10.1369/00221554261438169","url":null,"abstract":"ADAMTS1 (a disintegrin-like and metalloproteinase domain with thrombospondin type 1 repeats) is a secreted metalloproteinase with a known role in extracellular matrix remodeling in cardiovascular development and female fertility. Because of conflicting report of embryonic lethality and survival in Adamts1 mutant mice, we report generation and characterization of a new knockout (KO) allele, which led to detection of neonatal lethal omphalocele (persistent umbilical hernia) as a new Adamts1-dependent birth defect. This phenotype was also detected in another mutants with an in-frame lacZ insertion. β-galactosidase staining in the latter showed that Adamts1 was strongly expressed in the undifferentiated cells in the developing ventral abdominal wall preceding midline fusion at the umbilical cord attachment site. The omphalocele was characterized by the accumulation of the proteoglycan versican along with reduced proteolysis and impaired development of the superficial muscle layer, the panniculus carnosus, around the site of umbilical cord attachment. Furthermore, we observed sustained expression of the transcription factor, paired-like homeodomain transcription factor 2 in KO embryos, whereas it was downregulated in wild-type embryos during late embryogenesis. ADAMTS1 is thus a new matrisome component required for body wall closure.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261438169"},"PeriodicalIF":1.5,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FGF2 Boost for Driving Forebrain Organoid Maturation Under Static Conditions. 静态条件下FGF2促进前脑类器官成熟

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-04-24 DOI: 10.1369/00221554261433071

Eleonora Grecu, Cristina Maxia, Cristina Dolciotti, Paolo Bongioanni, Renata Del Carratore, Michela Isola, Ignazia Mocci, Maria Antonietta Casu, Federico Picciau, Daniela Murtas, Andrea Diana

{"title":"FGF2 Boost for Driving Forebrain Organoid Maturation Under Static Conditions.","authors":"Eleonora Grecu, Cristina Maxia, Cristina Dolciotti, Paolo Bongioanni, Renata Del Carratore, Michela Isola, Ignazia Mocci, Maria Antonietta Casu, Federico Picciau, Daniela Murtas, Andrea Diana","doi":"10.1369/00221554261433071","DOIUrl":"https://doi.org/10.1369/00221554261433071","url":null,"abstract":"Forebrain organoids (FOs) closely replicate key features of human brain, but often develop necrotic cores due to oxygen diffusion limits, resulting in disassembly. While dynamic culture devices can mitigate this, they add variability and diverge from adult cerebral static environment. Inspired by allometric scaling principles of brain growth, we developed a uniform, static culture protocol applying a 1-day transient high-dose Fibroblast Growth Factor 2 (100 ng/ml) treatment before neural induction. This acted as a proliferative stimulus, promoting long-term viability and structural integrity. Using human embryonic stem cells, early-FOs with diameters of 500-1000 µm achieved an 83.33% survival rate at 20 days in vitro (DIV). Area and volume increased significantly during 60 DIV culture period, but between 30 and 60 DIV they plateaued, indicating a transition from neural development to maturation. Weight increased until 30 DIV, but it significantly dropped between 30 and 60 DIV, possibly reflecting the formation of lumen-like structures. At 60 DIV, immunofluorescence revealed organized PAX6+ ventricle-like structures, where SOX2 marked neural progenitors, TUJ1 and MAP2 indicated mature neurons, GFAP identified astrocytes, and SYN1 highlighted emerging synaptic networks. This scalable protocol supports robust FOs generation, providing optimization and practical improvement within established frameworks to advance precision medicine.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261433071"},"PeriodicalIF":1.5,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13109269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Glial Cell Line-Derived Neurotrophic Factor and Ciliary Neurotrophic Factor on Extraocular and Limb Muscle Precursor Cells. 神经胶质细胞系源性神经营养因子和睫状体神经营养因子对眼外和肢体肌前体细胞的影响。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-04-13 DOI: 10.1369/00221554261435666

Austin J Winker, Laura L Johnson, Linda K McLoon

{"title":"Effects of Glial Cell Line-Derived Neurotrophic Factor and Ciliary Neurotrophic Factor on Extraocular and Limb Muscle Precursor Cells.","authors":"Austin J Winker, Laura L Johnson, Linda K McLoon","doi":"10.1369/00221554261435666","DOIUrl":"10.1369/00221554261435666","url":null,"abstract":"SummaryMyogenic precursor cells within skeletal muscles are responsible for the maintenance of skeletal muscle over a lifetime. Neurotrophic and growth factors play critical roles in this maintenance and in responses of myogenic precursor cells. Both glial cell line-derived neurotrophic factor (GDNF) and ciliary neurotrophic factor (CNTF) play roles in the maintenance and/or development of strabismus, yet few studies have examined their roles in the control of myogenic precursor cell proliferation and differentiation. Two populations of myogenic precursor cells were isolated from extraocular and leg muscle by fluorescence-activated cell sorting: EECD34 cells, largely PITX2-positive, and PAX7-positive cells. Cultures were treated with GDNF or CNTF and processed immunohistochemically to determine proliferation and differentiation rates. Neither GDNF nor CNTF affected cell proliferation rates for either muscle. Both treatments impacted cell differentiation by increasing multinucleated cell number, with TA-derived precursor cells producing cells containing large numbers of nuclei and EOM-derived precursor cells producing shorter multinucleated fibers with fewer nuclei. These differences may explain the presence of extremely short myofibers within normal adult EOM compared with limb muscle. As GDNF and CNTF are downregulated in strabismic muscles, data suggest that myofiber length homeostasis may be disrupted in strabismic EOM and suggest possible approaches for strabismus treatment.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261435666"},"PeriodicalIF":1.5,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13076467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147674202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contribution of Cardiac CD34⁺ Stromal Cells to Post-Myocardial Infarction Repair in Middle-Aged Rats. 心肌CD34 +基质细胞在中年大鼠心肌梗死后修复中的作用

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-04-12 DOI: 10.1369/00221554261434317

Daniel T Schneider, Eduard I Dedkov

{"title":"Contribution of Cardiac CD34⁺ Stromal Cells to Post-Myocardial Infarction Repair in Middle-Aged Rats.","authors":"Daniel T Schneider, Eduard I Dedkov","doi":"10.1369/00221554261434317","DOIUrl":"10.1369/00221554261434317","url":null,"abstract":"This study investigated the spatiotemporal dynamics of cardiac CD34⁺ stromal cells (SCs) during the reparative/proliferative phase of post-myocardial infarction (MI) healing. A transmural, non-reperfused MI was induced in middle-aged male Sprague-Dawley rats via left anterior coronary artery ligation, and proliferating cells were labeled with 5-bromo-2'-deoxyuridine. Hearts were collected at days 3, 7, and 14 after MI and analyzed using histology and immunohistochemistry. We found that the myocardial interstitium and coronary vessel adventitia harbored a population of cardiac CD34⁺ SCs. Following MI, activated CD34⁺ SCs expanded from the peri-infarct region across the healing wound through proliferation and migration, often alongside activated fibroblasts/myofibroblasts. While α-SMA⁺ myofibroblasts accumulated at pro-fibrotic granulation tissue sites, CD34⁺ SCs preferentially repopulated residual endomysial scaffolds spared by phagocytic macrophages. Over time, expanding fibrotic tissue progressively overtook these regions, leading to disappearance of CD34⁺ SCs. Importantly, clusters of CD34⁺ SCs accumulated at the scar border around the stumps of surviving cardiac myocytes, seemingly facilitating integration of endomysial connective tissue from non-infarcted myocardium into the developing fibrotic scar matrix. Collectively, these findings suggest that, unlike α-SMA⁺ myofibroblasts, cardiac CD34⁺ SCs seemed to support regenerative rather than fibrotic repair during post-MI wound healing by contributing to the preservation of myocardial stromal architecture.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261434317"},"PeriodicalIF":1.5,"publicationDate":"2026-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13070992/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147674061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Type and Size of Fluorescent Proteins Modulate the Localization of PTEN and Its Fragments, but Have Little or No Effect on Mutant PTEN or Stressed Cells. 荧光蛋白的类型和大小调节PTEN及其片段的定位，但对突变PTEN或应激细胞的影响很小或没有影响。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-27 DOI: 10.1369/00221554261434309

Takashi Kato, Yuuri Kawakami

{"title":"Type and Size of Fluorescent Proteins Modulate the Localization of PTEN and Its Fragments, but Have Little or No Effect on Mutant PTEN or Stressed Cells.","authors":"Takashi Kato, Yuuri Kawakami","doi":"10.1369/00221554261434309","DOIUrl":"10.1369/00221554261434309","url":null,"abstract":"The functions of phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor suppressor, depend on its subcellular localization. At the plasma membrane, PTEN dephosphorylates phosphatidylinositol-3,4,5-triphosphate to inhibit AKT signaling, whereas nuclear PTEN contributes to the maintenance of genomic stability. Fluorescent proteins (FPs) are widely used to assess PTEN's subcellular localization; however, both the intrinsic properties of FPs (e.g., molecular size) and the choice of FP can influence subcellular localization. This study aimed to determine whether FP fusion affects the subcellular localization of PTEN and its mutant forms under conditions involving DNA damage. mCherry typically promotes cytosolic localization of FP-fused PTEN, indicating that FP selection may affect the interpretation of localization data. Furthermore, FP fusion increases the molecular size of the truncated PTEN fragment, which may impede its nuclear import. In comparison, PTEN mutants such as PTENK13R or PTENA4, which predominantly localize to the cytoplasm or nucleus, respectively, show a minimal dependence on the type of FP. Similarly, DNA damage-induced nuclear accumulation of PTEN appears to be independent of the FP type. These findings underscore the importance of carefully considering the effects of FP fusion when investigating the mechanisms regulating the nuclear translocation of PTEN.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261434309"},"PeriodicalIF":1.5,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13033038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147530043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of Macrophage Migration Inhibitory Factor (MIF) Alters the Timing of Ventral Prostate Maturation in Mice. 巨噬细胞迁移抑制因子（MIF）的缺失改变了小鼠腹侧前列腺成熟的时间。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-25 DOI: 10.1369/00221554261431897

Júlia Eduarda Mesquita Matos, Marina das Graças Carneiro E Silva, Laura Eduarda Dinato Sudário, Luiz Felipe Fernandes Peixoto, Renata Graciele Zanon, Daniele Lisboa Ribeiro

{"title":"Loss of Macrophage Migration Inhibitory Factor (MIF) Alters the Timing of Ventral Prostate Maturation in Mice.","authors":"Júlia Eduarda Mesquita Matos, Marina das Graças Carneiro E Silva, Laura Eduarda Dinato Sudário, Luiz Felipe Fernandes Peixoto, Renata Graciele Zanon, Daniele Lisboa Ribeiro","doi":"10.1369/00221554261431897","DOIUrl":"10.1369/00221554261431897","url":null,"abstract":"This study investigated the contribution of macrophage migration inhibitory factor (MIF) for the development of ventral prostate in pubertal and adult mice. Mice aged 30 or 60 days from C57BL/6 WT (wild-type) and MIF-/-(knockout) strains were studied. Histological analysis, immunohistochemistry (smooth muscle alpha-actin, vimentin, PCNA, WNT5a), serum testosterone, and western blotting for ERK1/2 were performed. Thirty-day-old MIF-/- mice exhibited higher testosterone serum levels, and the ventral prostate presented enlarged luminal area as well as decreased collagen and smooth muscle cell content. Regarding cell proliferation, there was an important reduction in MIF-/- mice of both ages. In addition, MIF-/- 30 mice presented elevated ERK activation and WNT5a scores in the prostate. This study showed that developmental change expected only at adulthood of prostate is already very evident at 30 days of age in MIF-/- mice, anticipating the pubertal development. Thus, MIF has a stimulative role in proliferation and also modulates androgenic stimuli in the prostate, which can contribute to gland development from puberty to adulthood.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261431897"},"PeriodicalIF":1.5,"publicationDate":"2026-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13021533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147512571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Chloride Channel Regulator, Calcium-Activated-1 Is Expressed in Synoviocytes and Articular Chondrocytes in Health and Disease. 健康和疾病中滑膜细胞和关节软骨细胞表达的氯离子通道调节剂钙活化-1

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-15 DOI: 10.1369/00221554261423720

Judith Bushe, Jenny Fürstenau, Florian Bartenschlager, Simon Dökel, Vladimir M Jovanovic, Gundula Rösch, Zsuzsa Jenei-Lanzl, Frank Zaucke, Kristina Dietert, Achim D Gruber

{"title":"The Chloride Channel Regulator, Calcium-Activated-1 Is Expressed in Synoviocytes and Articular Chondrocytes in Health and Disease.","authors":"Judith Bushe, Jenny Fürstenau, Florian Bartenschlager, Simon Dökel, Vladimir M Jovanovic, Gundula Rösch, Zsuzsa Jenei-Lanzl, Frank Zaucke, Kristina Dietert, Achim D Gruber","doi":"10.1369/00221554261423720","DOIUrl":"10.1369/00221554261423720","url":null,"abstract":"The chloride channel regulator, calcium-activated-1, CLCA1, acts as a multifunctional secreted glycoprotein in anion channel modulation, mucus homeostasis, immune regulation, and other functions. So far, it has been described in goblet and other mucus-producing cells in mucous membranes, primarily of the respiratory, alimentary, and urogenital tracts. Here, we identify the expression of CLCA1 in fibroblast-like synoviocytes and superficial as well as, to a lesser extent, intermediate zone chondrocytes of diarthrodial articular joints. The expression pattern was found to be conserved in major joints in mice, pigs, and humans. First analyses of degenerate or infectious inflammatory joint conditions in mice or pigs, respectively, suggest rather continuous expression levels in articular disease. We speculate that CLCA1 may be involved in articular anion conductance, proteoglycan homeostasis, and inflammation of articular joints, possibly similar to analogous functions of this molecule as established in mucous membranes of the lungs and intestine.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261423720"},"PeriodicalIF":1.5,"publicationDate":"2026-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12989442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147463379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-step Gelatin Embedding Preserves Fibrocartilage Morphology, Enthesis, and Cartilage Stain of Bone Joints. 两步明胶包埋可保留骨关节的纤维软骨形态、骨骺和软骨染色。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-10 DOI: 10.1369/00221554261422633

Rafael Correia Cavalcante, Tongqing Zhou, Nidhi Bhandari, Zachary Rickman, Peter X Ma, Yuji Mishina

{"title":"Two-step Gelatin Embedding Preserves Fibrocartilage Morphology, Enthesis, and Cartilage Stain of Bone Joints.","authors":"Rafael Correia Cavalcante, Tongqing Zhou, Nidhi Bhandari, Zachary Rickman, Peter X Ma, Yuji Mishina","doi":"10.1369/00221554261422633","DOIUrl":"10.1369/00221554261422633","url":null,"abstract":"Maintaining the native morphology and characteristic staining patterns of dissected tissues is critical for histological analysis. Reliable preservation enables accurate assessment of structural integrity and cellular components, which is fundamental to identifying viable avenues for tissue regeneration and improving clinical outcomes. Acknowledging the constraints of conventional paraffin embedding, we propose an alternative method employing gelatin as an embedding medium. Gelatin is a natural, water-soluble protein derived from collagen, known for its biocompatibility. As a hydrogel, it provides a supportive matrix that closely mimics the natural extracellular matrix of soft tissues facilitating excellent preservation of structural and molecular features during the histological process. Here we demonstrate that this strategy offers superior support for fragile tissues, including fibrocartilage and hyaline cartilage across various anatomical sites, such as the temporomandibular joint, the knee joint, the long bone growth plate, and the intervertebral disc. To assess the maintenance of tissue morphology and matrix composition, histological sections were subjected to various staining techniques, including hematoxylin and eosin, Masson's trichome, safranin O, immunofluorescence, and von Kossa staining. Gelatin embedding resulted in superior maintenance of fibrocartilage architecture, as evidenced by histological evaluation. In addition, quantification of safranin O staining showed significantly greater glycosaminoglycan content in gelatin-embedded samples.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261422633"},"PeriodicalIF":1.5,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12975546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147433409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Triple Staining Including FOXA2 Identifies Stem Cell Lineages Undergoing Hepatic and Biliary Differentiation in Cirrhotic Human Liver". “包括FOXA2在内的三重染色识别肝硬化人类肝脏和胆道分化的干细胞谱系”的勘误表。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-10 DOI: 10.1369/00221554261432912

引用次数: 0