Journal of Histochemistry & Cytochemistry最新文献

Short-Term Treatment of Melatonin Improves the Expression of Cell Adhesion Molecules in the Testis of the Mouse Cryptorchidism Model. 褪黑素短期治疗可改善隐睾模型小鼠睾丸中细胞粘附分子的表达

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-09-20 DOI: 10.1369/00221554241279505

Arunothai Wanta, Kazuhiro Noguchi, Taichi Sugawara, Kayoko Sonoda, Keerakarn Somsuan, Tomohiko Wakayama

{"title":"Short-Term Treatment of Melatonin Improves the Expression of Cell Adhesion Molecules in the Testis of the Mouse Cryptorchidism Model.","authors":"Arunothai Wanta, Kazuhiro Noguchi, Taichi Sugawara, Kayoko Sonoda, Keerakarn Somsuan, Tomohiko Wakayama","doi":"10.1369/00221554241279505","DOIUrl":"https://doi.org/10.1369/00221554241279505","url":null,"abstract":"Melatonin plays a major role in regulating the sleep-wake cycle and enhancing testosterone production. We investigated the short-term effects of melatonin treatment for 14 consecutive days in the cryptorchidism model. We categorized experimental mice into Sham (S), Orchiopexy (O), Melatonin (Mel), and Orchiopexy + Melatonin (OMel) groups. Surgery involved inducing cryptorchidism in the left testis for seven days, followed by orchiopexy. The Mel group's testes did not descend, but they received melatonin injections after seven days of cryptorchidism. The OMel group underwent both orchiopexy and melatonin treatment. Both O and Mel groups exhibited decreased sperm and round-headed sperm in the epididymis. Significant increases were observed in the numbers of giant cells and negative Nectin-3 cells at p-value<0.05. The pattern of Cadm1 expression changed, and Nectin-2 and Nectin-3 co-expression was lacking in abnormal spermatids. Sertoli cell cytoplasm in both O and Mel groups exhibited autophagosomes and multivesicular bodies, which correlated with increased cyclooxygenase-2 expression. However, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cell numbers increased significantly in all treatment groups compared to the S group. Our study found that the combination of orchiopexy and melatonin positively influenced the expression of cell adhesion molecules (Cadm1, Nectin-2, and Nectin-3) involved in spermatogenesis, while reducing giant cells, autophagosomes, and apoptosis.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Expression of Membrane-bound Carbonic Anhydrases IV, IX, and XIV in the Mouse Heart". 膜结合碳酸酐酶 IV、IX 和 XIV 在小鼠心脏中的表达 "的更正。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-09-18 DOI: 10.1369/00221554241284816

引用次数: 0

Renal Mast Cell-Specific Proteases in the Pathogenesis of Tubulointerstitial Fibrosis. 肾脏肥大细胞特异性蛋白酶在肾小管间质纤维化发病机制中的作用

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-09-12 DOI: 10.1369/00221554241274878

Dmitrii Atiakshin,Sergey Morozov,Vladimir Dlin,Andrey Kostin,Artem Volodkin,Michael Ignatyuk,Galina Kuzovleva,Sergey Baiko,Irina Chekmareva,Svetlana Chesnokova,Daniel Elieh-Ali-Komi,Igor Buchwalow,Markus Tiemann

{"title":"Renal Mast Cell-Specific Proteases in the Pathogenesis of Tubulointerstitial Fibrosis.","authors":"Dmitrii Atiakshin,Sergey Morozov,Vladimir Dlin,Andrey Kostin,Artem Volodkin,Michael Ignatyuk,Galina Kuzovleva,Sergey Baiko,Irina Chekmareva,Svetlana Chesnokova,Daniel Elieh-Ali-Komi,Igor Buchwalow,Markus Tiemann","doi":"10.1369/00221554241274878","DOIUrl":"https://doi.org/10.1369/00221554241274878","url":null,"abstract":"Chronic kidney disease is detected in 8-15% of the world's population. Along with fibrotic changes, it can lead to a complete loss of organ function. Therefore, a better understanding of the onset of the pathological process is required. To address this issue, we examined the interaction between mast cells (MCs) and cells in fibrous and intact regions, focusing on the role of MC proteases such as tryptase, chymase, and carboxypeptidase A3 (CPA3). MCs appear to be involved in the development of inflammatory and fibrotic changes through the targeted secretion of tryptase, chymase, and CPA3 to the vascular endothelium, nephron epithelium, interstitial cells, and components of intercellular substances. Protease-based phenotyping of renal MCs showed that tryptase-positive MCs were the most common phenotype at all anatomic sites. The infiltration of MC in different anatomic sites of the kidney with an associated release of protease content was accompanied by a loss of contact between the epithelium and the basement membrane, indicating the active participation of MCs in the formation and development of fibrogenic niches in the kidney. These findings may contribute to the development of novel strategies for the treatment of tubulointerstitial fibrosis.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142194286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Making Multiplexed Imaging Flexible: Combining Essential Markers With Established Antibody Panels. 让多重成像更灵活：将基本标记物与成熟的抗体组相结合。

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI: 10.1369/00221554241274856

Ashik Jawahar Deen, Johan Thorsson, Eleanor M O'Roberts, Pranauti Panshikar, Tony Ullman, David Krantz, Carolina Oses, Charlotte Stadler

{"title":"Making Multiplexed Imaging Flexible: Combining Essential Markers With Established Antibody Panels.","authors":"Ashik Jawahar Deen, Johan Thorsson, Eleanor M O'Roberts, Pranauti Panshikar, Tony Ullman, David Krantz, Carolina Oses, Charlotte Stadler","doi":"10.1369/00221554241274856","DOIUrl":"10.1369/00221554241274856","url":null,"abstract":"Multiplexed immunofluorescence (IF) can be achieved using different commercially available platforms, often making use of conjugated antibodies detected in iterative cycles. A growing portfolio of pre-conjugated antibodies is offered by the providers, as well as the possibility for in-house conjugation. For many conjugation methods and kits, there are limitations in which antibodies can be used, and conjugation results are sometimes irreproducible. The conjugation process can limit or slow down the progress of studies requiring conjugation of essential markers needed for a given project. Here, we demonstrate a protocol combining manual indirect immunofluorescence (IF) of primary antibodies, followed by antibody elution and staining with multiplexed panels of commercially pre-conjugated antibodies on the PhenoCycler platform. We present detailed protocols for applying the workflow on fresh frozen and formalin fixed paraffin embedded tissue sections. We also provide a ready to use workflow for coregistration of the images and demonstrate this for two examples.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Expression of Branched-Chain Aminotransferase in the Rat Ocular Tissues. 支链氨基转移酶在大鼠眼组织中的差异表达

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-08-01 Epub Date: 2024-08-30 DOI: 10.1369/00221554241272338

Dalia I Aldosari, Yasser A Alshawakir, Ibrahim O Alanazi, Abdullah S Alhomida, Mohammad S Ola

{"title":"Differential Expression of Branched-Chain Aminotransferase in the Rat Ocular Tissues.","authors":"Dalia I Aldosari, Yasser A Alshawakir, Ibrahim O Alanazi, Abdullah S Alhomida, Mohammad S Ola","doi":"10.1369/00221554241272338","DOIUrl":"10.1369/00221554241272338","url":null,"abstract":"Branched-chain amino acids (BCAAs) play vital roles in metabolic and physiological processes, with their catabolism initiated by two branched-chain aminotransferase isozymes: cytosolic (BCATc) and mitochondrial (BCATm). These enzymes have tissue and cell-specific compartmentalization and are believed to shuttle metabolites between cells and tissues. Although their expression and localization have been established in most tissues, ocular tissues remain unknown. In this study, we used immunohistochemical analyses to investigate the expression and localization of BCAT enzymes in the normal eye tissues. As expected, BCATc was highly expressed in the neuronal cells of the retina, particularly in the ganglion cell layers, inner nuclear layer, and plexiform layer, with little to no expression in Müller cells. BCATc was also present in the cornea, retinal pigment epithelium (RPE), choroid, ciliary body, and iris but not in the lens. In contrast, BCATm was expressed across all ocular tissues, with strong expression in the Muller cells of the retina, the endothelial and epithelial layers of the cornea, the choroid and iris, and the epithelial cells at the lens's front. The extensive expression and distribution of BCAT isozymes in the ocular tissue, suggests that BCAA transamination is widespread in the eye, potentially aiding in metabolite transport between ocular tissues. The findings provide new insights into the physiological role of BCATs in the eye, particularly within the neuronal retina.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142108114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Controversy Regarding the Identity of the Enzyme That Mediates Glucagon-Like Peptide 1 Synthesis in Human Alpha Cells. 关于人类α细胞中促进胰高血糖素样肽 1 合成的酶的身份的争议。

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-08-01 Epub Date: 2024-09-09 DOI: 10.1369/00221554241274879

Gladys Teitelman

{"title":"A Controversy Regarding the Identity of the Enzyme That Mediates Glucagon-Like Peptide 1 Synthesis in Human Alpha Cells.","authors":"Gladys Teitelman","doi":"10.1369/00221554241274879","DOIUrl":"10.1369/00221554241274879","url":null,"abstract":"Processing of proglucagon into glucagon-like peptide-1 (GLP-1) and GLP-2 in intestinal L cells is mediated by the prohormone convertase 1/3 (PC1/3) while PC2 is responsible for the synthesis of glucagon in pancreatic alpha cells. While GLP-1 is also produced by alpha cells, the identity of the convertase involved in its synthesis is still unsettled. It also remains to be determined whether all alpha cells produce the incretin. The aims of this study were first, to elucidate the identity of the proconvertase responsible for GLP-1 production in human alpha cells, and second, to ascertain whether the number of glucagon cells expressing GLP-1 increase during diabetes. To answer these questions, sections of pancreas from donors' non-diabetic controls, type 1 and type 2 diabetes were processed for double-labelled immunostaining of glucagon and GLP-1 and of each hormone and either PC1 or PC2. Stained sections were examined by confocal microscopy. It was found that all alpha cells of islets from those three groups expressed GLP-1 and PC2 but not PC1/3. This observation supports the view that PC2 is the convertase involved in GLP-1 synthesis in all human glucagon cells and suggests that the regulation of its activity may have important clinical application in diabetes.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Frequent Icing Stimulates Skeletal Muscle Regeneration Following Injury With Necrosis in a Small Fraction of Myofibers in Rats. 频繁结冰可刺激大鼠损伤后的骨骼肌再生，并使一小部分肌纤维坏死。

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-08-01 Epub Date: 2024-09-06 DOI: 10.1369/00221554241274882

Masato Kawashima, Itsuki Nagata, Erika Terada, Asano Tamari, Mami Kurauchi, Tohma Sakuraya, Takahiro Sonomura, Eri Oyanagi, Hiromi Yano, Jonathan M Peake, Takamitsu Arakawa

{"title":"Frequent Icing Stimulates Skeletal Muscle Regeneration Following Injury With Necrosis in a Small Fraction of Myofibers in Rats.","authors":"Masato Kawashima, Itsuki Nagata, Erika Terada, Asano Tamari, Mami Kurauchi, Tohma Sakuraya, Takahiro Sonomura, Eri Oyanagi, Hiromi Yano, Jonathan M Peake, Takamitsu Arakawa","doi":"10.1369/00221554241274882","DOIUrl":"10.1369/00221554241274882","url":null,"abstract":"Icing interventions on the injured skeletal muscle affect the macrophage-related regenerative events and muscle repair. However, despite its importance for the practice in sport medicine, the influence of different icing protocols on muscle regeneration remains unclear. Here, using a rodent model of mild muscle injury with necrosis in a small fraction of myofibers, the injured animals were allocated to four groups: non-icing control (Con) and a single treatment (Ice-1), three treatments (Ice-3), or nine treatments (Ice-9) with a 30-min icing each time within two days following injury. Muscle regeneration was compared between the groups on post-injury days 1, 3, 5, and 7. The results showed that compared with the Con group, muscle regeneration was faster in the Ice-9 group (but not in the Ice-1 and Ice-3 groups), as indicated by more rapid accumulation of satellite cells within the regenerating area and enlarged size of regenerating myofibers (p<0.05, respectively). There was also less macrophage accumulation (p<0.05) and a trend toward early removal of necrotic myofibers in the damaged/regenerating area in the Ice-9 group (p=0.0535). These results demonstrate that in the case of mild muscle damage, more frequent icing treatment is more effective to stimulate muscle regeneration.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histone Acetyl Transferase 1 Is Overexpressed in Poor Prognosis, High-grade Meningeal and Glial Brain Cancers: Immunohistochemical and Aptahistochemical Study. 组蛋白乙酰转移酶 1 在预后不良的高级别脑膜癌和胶质脑癌中过度表达：免疫组织化学和表观组织化学研究。

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-08-01 Epub Date: 2024-08-24 DOI: 10.1369/00221554241272341

Sandra Bargiela-Cuevas, María Marin, María Gabaldon-Ojeda, José I Klett-Mingo, Paula Granado, Silvia Sacristan, Alfonso Esteban-Lasso, José G Casas, María E Martin, Victor M M González, Mar Royuela, Ignacio García-Tuñon, Miguel Angel Ortega Núñez, María Del Val Toledo Lobo

{"title":"Histone Acetyl Transferase 1 Is Overexpressed in Poor Prognosis, High-grade Meningeal and Glial Brain Cancers: Immunohistochemical and Aptahistochemical Study.","authors":"Sandra Bargiela-Cuevas, María Marin, María Gabaldon-Ojeda, José I Klett-Mingo, Paula Granado, Silvia Sacristan, Alfonso Esteban-Lasso, José G Casas, María E Martin, Victor M M González, Mar Royuela, Ignacio García-Tuñon, Miguel Angel Ortega Núñez, María Del Val Toledo Lobo","doi":"10.1369/00221554241272341","DOIUrl":"10.1369/00221554241272341","url":null,"abstract":"Primary malignancies of the central nervous system account for 2% of all cancers in adults and almost 15% in children under 15 years of age. The prognosis of brain anaplastic cancers and glioblastomas remains extremely poor, with devastating survival expectative, and new molecular markers and therapeutic targets are essential. Epigenetic changes constitute an extensive field for the development of new diagnostic and therapeutic strategies. Histone acetyl transferase-1 (HAT1) has merged as a potential prognostic marker and therapy target for different malignancies. Data repository analysis showed HAT1 mRNA overexpression in gliomas and has been described its alternative splicing in glioblastomas. Using immunohistochemical and aptahistochemical methods, we analyzed the expression of HAT1 in meningiomas, oligodendrogliomas, and astroglial cancers. We observed that HAT1 overexpression is associated with the most aggressive tumor types and the worse prognosis, as well as with a higher probability of early relapse in meningiomas. Its cytosolic localization correlates with tumor progression and prognosis. Aptamers, synthetic oligonucleotides capable to bind and inhibit a wide variety of targets, are considered as promising diagnostic and therapeutic tools. Aptahistochemistry using the aptamer apHAT610 offered superior results in comparison with the antibody used, as a good example of the potential of aptamers as diagnostic tools for histopathology.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Podocalyxin Potentially Decorated With Low-sulfated Keratan Sulfate in Human Testicular Embryonal Carcinoma. 人睾丸胚胎癌中可能装饰有低硫酸化角叉菜胶的荚膜萼蛋白的表达

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-07-01 Epub Date: 2024-07-25 DOI: 10.1369/00221554241265162

Akifumi Muramoto, Hitomi Hoshino, So Inamura, Masataka Murahashi, Tomoya O Akama, Naoki Terada, Motohiro Kobayashi

{"title":"Expression of Podocalyxin Potentially Decorated With Low-sulfated Keratan Sulfate in Human Testicular Embryonal Carcinoma.","authors":"Akifumi Muramoto, Hitomi Hoshino, So Inamura, Masataka Murahashi, Tomoya O Akama, Naoki Terada, Motohiro Kobayashi","doi":"10.1369/00221554241265162","DOIUrl":"10.1369/00221554241265162","url":null,"abstract":"SummaryWe previously demonstrated that among various histological types of human testicular germinal cell tumors (GCTs), embryonal carcinoma (EC) preferentially expresses low-sulfated keratan sulfate (KS) consisting of repeating N-acetyllactosamine (LacNAc) disaccharide units composed of galactose and 6-O-sulfated N-acetylglucosamine (GlcNAc), which is recognized by the R-10G antibody. Recently, we generated another anti-low-sulfated KS monoclonal antibody, 294-1B1. Immunohistochemical analysis of testicular GCTs (n=83) revealed that the low-sulfated KS recognized by 294-1B1 is also preferentially expressed in EC but minimally in other GCT histological types. Moreover, immunolabeling with R-10G and 294-1B1 antibodies was resistant to peptide-N-glycosidase F digestion, and EC was not stained with the MECA-79 antibody, indicating that low-sulfated KS expressed in EC contains mucin-type core 2 O-glycans carrying GlcNAc-6-O-sulfated oligo-LacNAc. Double immunofluorescence staining showed that R-10G and 294-1B1 antibody signals colocalized with those for podocalyxin (PODXL). Furthermore, western blot analysis of recombinant human PODXL•IgG fusion proteins secreted from low-sulfated KS-expressing human embryonic kidney 293T cells revealed that PODXL functions as a core protein for low-sulfated KS. Taken together, these findings strongly suggest that the PODXL glycoform decorated with low-sulfated KS is preferentially expressed in human testicular EC and may therefore serve as a diagnostic marker for this malignancy.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11308194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Normal Plasma Cell Distribution Across Distinct Age Cohorts Reveals Age-Dependent Changes. 对不同年龄组正常浆细胞分布的分析揭示了随年龄而发生的变化。

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-07-01 Epub Date: 2024-07-25 DOI: 10.1369/00221554241266005

Denise K Walters, Diane F Jelinek

{"title":"Analysis of Normal Plasma Cell Distribution Across Distinct Age Cohorts Reveals Age-Dependent Changes.","authors":"Denise K Walters, Diane F Jelinek","doi":"10.1369/00221554241266005","DOIUrl":"10.1369/00221554241266005","url":null,"abstract":"Hematopoietic and stromal cells within the bone marrow (BM) provide membrane-bound and/or soluble factors that are vital for the survival of plasma cells (PCs). Recent reports in murine BM demonstrated the dynamic formation and dispersion of PC clusters. To date, PC clustering in normal human BM has yet to be thoroughly examined. The goal of this study was to determine whether PC clusters are present in human BM and whether clustering changes as a function of age. Quantification of PCs and clustering in BM sections across six different age groups revealed that fewer PCs and PC clusters were observed in the youngest and oldest age groups. PC clustering increased with age until the sixth decade and then began to decrease. A positive correlation between the number of PCs and PC clusters was observed across all age groups. PC clusters were typically heterogeneous for immunoglobulin heavy- and light-chain expression. Taken together, these data demonstrate that PC clusters are present in human BM and that PC clustering increases until middle adulthood and then begins to diminish. These results suggest the spatial distribution of BM PC-supportive stromal cells changes with age.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11308192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0