Journal of Histochemistry & Cytochemistry最新文献

Histo-LOOP: A novel embedding tool for standardizing, simplifying, and advancing histological tissue preparation.

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2025-03-29 DOI: 10.1369/00221554251329978

Pakhwan Nilcham, Mamdouh Afify, Nicole Schaaps, Carolina Neu, Rahma Shahin, Andreas Prescher, Felix Jan Vogt, Anne Turoni-Glitz

{"title":"Histo-LOOP: A novel embedding tool for standardizing, simplifying, and advancing histological tissue preparation.","authors":"Pakhwan Nilcham, Mamdouh Afify, Nicole Schaaps, Carolina Neu, Rahma Shahin, Andreas Prescher, Felix Jan Vogt, Anne Turoni-Glitz","doi":"10.1369/00221554251329978","DOIUrl":"10.1369/00221554251329978","url":null,"abstract":"SummaryTissue preparation for paraffin embedding is a crucial step in histological processes. Standardized methods are required to ensure the accuracy of research and clinical diagnostic results. However, standardization is particularly challenging for long luminal tissues. Conventional methods such as single/serial sections and the Swiss roll, often have drawbacks including the risk of missing or misaligned sections, excess consumption of materials, and high workload. They also require significant expertise and are difficult to standardize. To address these issues, we developed the Histo-LOOP embedding tool-a novel tool designed to standardize, simplify, and improve histological processing. Histo-LOOP is suitable for various tissue types including long tubular tissue, allowing for a complete overview in cross-sectional and longitudinal views. It is also suitable for punch biopsies or small sections, and enables the assessment of multiple punch biopsies or sections within one paraffin block, and in multiple cutting planes, for example, for liver and prostate biopsies. Histo-LOOP does not interfere with the sectioning and staining process and does not cause artifacts.Here, we introduce the novel tool Histo-LOOP and describe preparation techniques for tubular tissue and small tissue samples using this tool, along with examples of their histological evaluation.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554251329978"},"PeriodicalIF":1.9,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955986/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143742897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Immunostaining Protocols for 3D Visualization of Pericytes in Human Retinal Flatmounts.

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2025-03-17 DOI: 10.1369/00221554251323655

Noëlle Bakker, Aïcha A Croes, Eva Prevaes, Cornelis J F van Noorden, Reinier O Schlingemann, Ingeborg Klaassen

{"title":"Development of Immunostaining Protocols for 3D Visualization of Pericytes in Human Retinal Flatmounts.","authors":"Noëlle Bakker, Aïcha A Croes, Eva Prevaes, Cornelis J F van Noorden, Reinier O Schlingemann, Ingeborg Klaassen","doi":"10.1369/00221554251323655","DOIUrl":"10.1369/00221554251323655","url":null,"abstract":"Vascular pericytes are widely present across the human body and crucial in regulating vascular flow, permeability, and homeostasis. In the human retina, pericytes are important for forming and maintaining the blood-retinal barrier, as well as for autoregulation of blood flow. Pericyte loss has been implicated in various pathological conditions. Visualization of pericytes by immunofluorescence (IF) staining provides valuable information on pericyte number, morphology, location, and on expression of anatomic and functional markers. However, species-specific differences in pericyte marker expression exist. In this study, we aimed to develop a novel IF co-staining protocol to detect the pericyte markers NG2, PDGFRβ, αSMA, CD13, and RFC1 in human retinal flatmounts. Unlike retinal sections, retinal flatmounts enable 3D visualization of pericyte distribution across the entire vascular network. Key optimizations included tailoring the fixation method, blocking buffer composition and antibody solvent, as well as using jasplakinolide to enhance αSMA detection. Our protocol successfully enabled double staining of NG2 and PDGFRβ, as well as αSMA and PDGFRβ, whereas CD13 and RFC1 expression was not detectable in human retinal flatmounts. This novel 3D IF protocol enhances in situ visualization of human retinal pericytes, enabling accurate studies of their role in vascular health and disease to aid targeted therapy development.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554251323655"},"PeriodicalIF":1.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11915233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Satellite DNA Amplification in Advanced Prostate Cancer Is Largely Independent From Euchromatic and Oncogene Amplicons.

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2025-03-17 DOI: 10.1369/00221554251323657

Anja Weise, Antonio Augusto Ornellas, Gilda Alves, Constanze Pentzold, Jenny Holler, Melanie Wolter, Elena Jamali, Bernhard Theis, Thomas Liehr

引用次数: 0

RNAscope Multiplex FISH Signal Assessment in FFPE and Fresh Frozen Tissues: The Effect of Archival Duration on RNA Expression.

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2025-01-01 Epub Date: 2025-01-23 DOI: 10.1369/00221554241311971

Ariestya Indah Permata Sari, Katherine Copeland, Pattarin Nuwongsri, Wiriya Pipatsakulroj, Artit Jinawath, Nipan Israsena, Panuwat Lertsittichai, Prakasit Chirappapha, Meng-Shin Shiao, Natini Jinawath

{"title":"RNAscope Multiplex FISH Signal Assessment in FFPE and Fresh Frozen Tissues: The Effect of Archival Duration on RNA Expression.","authors":"Ariestya Indah Permata Sari, Katherine Copeland, Pattarin Nuwongsri, Wiriya Pipatsakulroj, Artit Jinawath, Nipan Israsena, Panuwat Lertsittichai, Prakasit Chirappapha, Meng-Shin Shiao, Natini Jinawath","doi":"10.1369/00221554241311971","DOIUrl":"10.1369/00221554241311971","url":null,"abstract":"Formalin-fixed paraffin-embedded tissue (FFPET), which is the most widely used pathology archive, usually has low-quality DNA and RNA due to extensive nucleic acid crosslinking. RNA fluorescence in situ hybridization (RNA-FISH) has been increasingly utilized in research and clinical settings to diagnose disease pathology. In this study, the effect of RNA degradation over archival time on RNA-FISH signals in FFPET and fresh frozen tissue (FFT) was systematically assessed. RNAscope multiplex fluorescent assay with the four house-keeping-gene (HKG) probes UBC, PPIB, POLR2A, and HPRT1 was performed on 62 archived breast cancer samples (30 FFPETs and 32 FFTs). As expected, the number of RNAscope signals in FFPETs is lower than in FFTs in an archival duration-dependent fashion. The RNA degradation in FFPETs is most pronounced in high-expressor HKGs, UBC and PPIB, than in low-to-moderate expressors POLR2A and HPRT1 (p<0.0001). Analysis of RNA expression over time showed that PPIB, which has the highest signal, was the most degraded in both adjusted transcript and H-score quantification methods (R2 = 0.35 and R2 = 0.33, respectively). This proves that although the RNAscope probes are designed to detect fragmented RNA, performing a sample quality check using HKGs is strongly recommended to ensure accurate results.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"9-28"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11755420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dominant Expression of Chromogranin B in Pituitary Corticotrophs and Its Putative Role in Interaction With Secretogranin III. 嗜铬颗粒蛋白B在垂体促肾上腺皮质激素中的显性表达及其与分泌颗粒蛋白III相互作用的推测作用。

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2025-01-01 Epub Date: 2025-01-10 DOI: 10.1369/00221554241311965

Shota Kikuchi, Koki Odashima, Tadashi Yasui, Seiji Torii, Masahiro Hosaka, Hiroshi Gomi

{"title":"Dominant Expression of Chromogranin B in Pituitary Corticotrophs and Its Putative Role in Interaction With Secretogranin III.","authors":"Shota Kikuchi, Koki Odashima, Tadashi Yasui, Seiji Torii, Masahiro Hosaka, Hiroshi Gomi","doi":"10.1369/00221554241311965","DOIUrl":"10.1369/00221554241311965","url":null,"abstract":"SummaryPrevious studies have suggested that chromogranin A (CgA) is a partner molecule of secretogranin III (SgIII). In mouse pituitary corticotroph-derived AtT-20 cells, SgIII plays a role in sorting CgA/hormone aggregates into secretory granules (SGs). Although CgA expression is equivocal, CgB is clearly detectable in the rat pituitary corticotrophs. Therefore, we hypothesized that CgB shares a function with CgA in pituitary corticotrophs. In the binding assays, CgB, similar to CgA, showed binding activity to SgIII under weakly acidic conditions and in the presence of Ca2+. Considering the differences in animal species, the different abilities of antibodies, and the conditions of tissue fixation and thin sectioning in immunofluorescence histochemistry, we found that CgA was expressed in a small population (approximately 10%), and its expression intensity was weaker than that of CgB (>98%) in rodent pituitary corticotrophs. In addition, similar to CgA, CgB and SgIII were colocalized in adrenocorticotropic hormone (ACTH) granules. The labeling of CgA and CgB was not completely consistent, and CgB colocalized with SgIII in many granules. These results suggest that there are multiple sorting systems for ACTH granules in pituitary corticotrophs and that the SgIII/CgB complex behaves more dominantly than the SgIII/CgA complex, which has somewhat different properties.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"29-53"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11719422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an ALK-positive Non-Small-Cell Lung Cancer in Vitro Tumor 3D Culture Model for Therapeutic Screening.

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2025-01-01 Epub Date: 2025-02-24 DOI: 10.1369/00221554251318435

Madeleine A Berry, Abigail R Bland, Gretel S Major, John C Ashton

{"title":"Development of an ALK-positive Non-Small-Cell Lung Cancer in Vitro Tumor 3D Culture Model for Therapeutic Screening.","authors":"Madeleine A Berry, Abigail R Bland, Gretel S Major, John C Ashton","doi":"10.1369/00221554251318435","DOIUrl":"10.1369/00221554251318435","url":null,"abstract":"Cancer cell monolayers are commonly used for preclinical drug screening. However, monolayers do not begin to mimic the complexity of the tumor microenvironment, including hypoxia and nutrient gradients within the tumor. To more accurately mimic solid tumors, we developed and drug-tested an anaplastic lymphoma kinase (ALK)-positive (H3122) non-small-cell lung cancer 3D (three-dimensional) culture model using light-activated gelatin methacryloyl hydrogels. We previously demonstrated that the combination of alectinib, an ALK inhibitor, and SHP099, an SHP2 inhibitor, had synergistic efficacy in ALK-positive cell monolayers. We aimed to test this drug combination in our novel ALK-positive 3D cancer model. We first validated the 3D cultures by comparing the distribution of terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells in the 3D cultures with sections from time-matched mouse xenografts, finding a comparable percentage of TUNEL-positive cells in the 3D culture and xenograft inner cores at each time point. When we investigated the effect of the combination of alectinib and SHP099 in these novel 3D cultures, we found a comparable cellular response compared with our two-dimensional experiments especially with the drugs in combination. We suggest that 3D cultures be used as preclinical screening platforms to ensure that only the most efficacious drug candidates move on to in vivo testing.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"63-79"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vascular E-selectin Expression Recognized by the U12-12 Monoclonal Antibody as a Predictive Marker for Steroid Resistance in Immune Checkpoint Inhibitor-related Colitis.

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2025-01-01 Epub Date: 2025-02-24 DOI: 10.1369/00221554251320703

Koki Nakashima, Tadanobu Nagaya, Mai Iwaya, Katsushi Hiramatsu, Takeshi Uehara, Yasunari Nakamoto, Tamotsu Ishizuka, Motohiro Kobayashi

{"title":"Vascular E-selectin Expression Recognized by the U12-12 Monoclonal Antibody as a Predictive Marker for Steroid Resistance in Immune Checkpoint Inhibitor-related Colitis.","authors":"Koki Nakashima, Tadanobu Nagaya, Mai Iwaya, Katsushi Hiramatsu, Takeshi Uehara, Yasunari Nakamoto, Tamotsu Ishizuka, Motohiro Kobayashi","doi":"10.1369/00221554251320703","DOIUrl":"10.1369/00221554251320703","url":null,"abstract":"In immune checkpoint inhibitor (ICI)-related colitis, tumor necrosis factor (TNF)-α blockade is recommended if symptoms are not alleviated with corticosteroids. Although TNF has been shown to be associated with steroid resistance, the early prediction of steroid resistance is challenging in clinical practice. Therefore, in the present study, we evaluated the potential of vascular E-selectin expression, which is induced by TNF, to serve as a predictor of steroid resistance in ICI-related colitis. We performed immunohistochemical analysis of 29 cases of ICI-related colitis using the U12-12 monoclonal antibody, which specifically recognizes E-selectin, and examined the association between U12-12 staining and clinical features. The result showed that the proportion of steroid-resistant cases was significantly higher in the U12-12-positive group (8 of 9 cases; 88.9%) than in the U12-12-negative group (1 of 20 cases; 5.0%) (p<0.001). Furthermore, the proportion of steroid-resistant cases was 100% (6 of 6 cases) in patients with U12-12-positive and Common Terminology Criteria for Adverse Events (CTCAE) grade 3 or higher and 0% (0 of 12 cases) in patients with U12-12-negativity and CTCAE grade 1 or 2. Thus, evaluation of vascular E-selectin expression using the U12-12 monoclonal antibody is useful for predicting steroid resistance in ICI-related colitis.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"81-88"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Glycoprotein 2 and Its Glycosylation in Human Cowper's Gland. 人类考伯氏腺中糖蛋白 2 的表达及其糖基化。

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2025-01-01 Epub Date: 2024-12-29 DOI: 10.1369/00221554241309413

Yusuke Fukiage, Shulin Low, Akifumi Muramoto, Yuzuru Ariga, Hitomi Hoshino, Tsutomu Nakada, Tomoya O Akama, Motohiro Kobayashi

{"title":"Expression of Glycoprotein 2 and Its Glycosylation in Human Cowper's Gland.","authors":"Yusuke Fukiage, Shulin Low, Akifumi Muramoto, Yuzuru Ariga, Hitomi Hoshino, Tsutomu Nakada, Tomoya O Akama, Motohiro Kobayashi","doi":"10.1369/00221554241309413","DOIUrl":"10.1369/00221554241309413","url":null,"abstract":"Glycoprotein 2 (GP2), initially identified as a primary membrane protein of zymogen granules in pancreatic acinar cells, is a marker of intestinal microfold cells (M cells) and involved in bacterial transcytosis in M cells. Recent studies have reported GP2 expression in the pancreas and intestine among various other organs. We aimed to elucidate the expression of GP2 and its glycosylation modifications in human Cowper's glands. We generated an anti-human GP2 monoclonal antibody suitable for formalin-fixed, paraffin-embedded tissue sections. This antibody, designated GP2-71, successfully stained both the plasma membrane and cytoplasm of normal pancreatic acinar cells. Subsequently, we performed GP2-71 immunostaining on normal human Cowper's gland tissues. Our findings revealed that the luminal cell membrane and contents of the Cowper's glands reacted strongly with GP2-71. Moreover, the regions stained with GP2-71 were partially immunostained with the anti-sialyl Lewis x (sLex) monoclonal antibody HECA-452. Furthermore, western blot analysis of protein A-purified GP2-immunoglobulin G fusion proteins demonstrated that GP2 was decorated with HECA-452-positive glycans. Collectively, these findings indicate that GP2 is expressed in human Cowper's glands and is potentially decorated with sLex-related glycans.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":"73 1-2","pages":"55-61"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11683828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of the Pancreatic Islet Microvasculature in Health and Disease. 胰岛微血管在健康和疾病中的作用

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-11-01 Epub Date: 2024-11-27 DOI: 10.1369/00221554241299862

Alfred C Aplin, Yasaman Aghazadeh, Olivia G Mohn, Rebecca L Hull-Meichle

{"title":"Role of the Pancreatic Islet Microvasculature in Health and Disease.","authors":"Alfred C Aplin, Yasaman Aghazadeh, Olivia G Mohn, Rebecca L Hull-Meichle","doi":"10.1369/00221554241299862","DOIUrl":"10.1369/00221554241299862","url":null,"abstract":"The pancreatic islet vasculature comprises microvascular endothelial cells surrounded by mural cells (pericytes). Both cell types support the islet by providing (1) a conduit for delivery and exchange of nutrients and hormones; (2) paracrine signals and extracellular matrix (ECM) components that support islet development, architecture, and endocrine function; and (3) a barrier against inflammation and immune cell infiltration. In type 2 diabetes, the islet vasculature becomes inflamed, showing loss of endothelial cells, detachment, and/or trans-differentiation of pericytes, vessel dilation, and excessive ECM deposition. While most work to date has focused either on endothelial cells or pericytes in isolation, it is very likely that the interaction between these cell types and disruption of that interaction in diabetes are critically important. In fact, dissociation of pericytes from endothelial cells is an early, key feature of microvascular disease in multiple tissues/disease states. Moreover, in beta-cell replacement therapy, co-transplantation with microvessels versus endothelial cells alone is substantially more effective in improving survival and function of the transplanted cells. Ongoing studies, including characterization of islet vascular cell signatures, will aid in the identification of new therapeutic targets aimed at improving islet function and benefiting people living with all forms of diabetes.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"711-728"},"PeriodicalIF":1.9,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11600425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

George Gomori's Contributions to Diabetes Research and the Origins of the Journal of Histochemistry and Cytochemistry. 乔治-戈莫里对糖尿病研究的贡献以及《组织化学与细胞化学杂志》的起源。

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-11-01 Epub Date: 2024-11-18 DOI: 10.1369/00221554241300370

Denis G Baskin

引用次数: 0