The Human Mechanosensory Corpuscles: A New Schwann Cell Localization of the Wilms' Tumor Protein WT1.

IF 1.9 4区 生物学 Q4 CELL BIOLOGY
Alfonso Cepeda-Emiliani, María Otero-Alén, Tomás García-Caballero, Rosalía Gallego, Lucía García-Caballero
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Abstract

SummaryThe Wilms' Tumor protein WT1 is a zinc-finger transcription factor with crucial roles in organogenesis, cell differentiation, tissue homeostasis, and oncogenesis. While its expression has been extensively studied in various tissues, its presence in the nervous system, particularly in peripheral glial cells, remains largely unexplored. In this study, we examined WT1 expression in the Schwann cells of mechanosensory corpuscles, nerve bundles, and free nerve endings (FNEs) within human penile tissues. Using single and double immunohistology, we analyzed WT1 coexpression with Schwann cell markers (S100, nestin, SOX10) and its association with axonal (neurofilaments, neuron-specific enolase, tyrosine hydroxylase) and perineurial/endoneurial markers (Glut-1, α-SMA, CD34). We found consistent WT1 cytoplasmic expression in the Schwann cells of Pacinian, Meissner, Krause, genital, Golgi-Mazzoni, and Ruffini-like corpuscles, with variable staining intensity. Confocal microscopy revealed WT1 colocalized with nestin but not S100, suggesting involvement in cytoskeletal organization. In addition, we documented WT1 in myelinating Schwann cells of nerve bundles, with distinct staining patterns in Cajal bands and Schmidt-Lanterman incisures, as well as in non-myelinating Schwann cells of FNEs. This is the first study to describe WT1 expression in sensory corpuscles, implicating it in Schwann cell development, maintenance, or plasticity, with potential relevance for peripheral nerve biology, pathology, and mechanosensation.

人机械感觉小体:Wilms肿瘤蛋白WT1的一种新的雪旺细胞定位。
Wilms肿瘤蛋白WT1是一种锌指转录因子,在器官发生、细胞分化、组织稳态和肿瘤发生中起着至关重要的作用。虽然其在各种组织中的表达已被广泛研究,但其在神经系统,特别是外周胶质细胞中的存在仍未被广泛探索。在这项研究中,我们检测了WT1在人阴茎组织机械感觉小体、神经束和自由神经末梢(FNEs)的雪旺细胞中的表达。通过单免疫和双免疫组织学,我们分析了WT1与雪旺细胞标记物(S100、巢蛋白、SOX10)的共表达,以及WT1与轴突(神经丝、神经元特异性烯醇化酶、酪氨酸羟化酶)和神经周围/神经内膜标记物(谷氨酸-1、α-SMA、CD34)的关联。我们发现WT1在Pacinian、Meissner、Krause、生殖器、Golgi-Mazzoni和ruffini样小体的雪旺细胞中表达一致,但染色强度不同。共聚焦显微镜显示WT1与巢蛋白共定位,但不与S100共定位,提示参与细胞骨架组织。此外,我们记录了WT1在神经束的有髓鞘的雪旺细胞中,在Cajal带和Schmidt-Lanterman切口以及FNEs的无髓鞘的雪旺细胞中具有不同的染色模式。这是第一个描述WT1在感觉小体中的表达的研究,暗示它与雪旺细胞的发育、维持或可塑性有关,与周围神经生物学、病理学和机械感觉具有潜在的相关性。
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来源期刊
CiteScore
6.00
自引率
0.00%
发文量
32
审稿时长
1 months
期刊介绍: Journal of Histochemistry & Cytochemistry (JHC) has been a pre-eminent cell biology journal for over 50 years. Published monthly, JHC offers primary research articles, timely reviews, editorials, and perspectives on the structure and function of cells, tissues, and organs, as well as mechanisms of development, differentiation, and disease. JHC also publishes new developments in microscopy and imaging, especially where imaging techniques complement current genetic, molecular and biochemical investigations of cell and tissue function. JHC offers generous space for articles and recognizing the value of images that reveal molecular, cellular and tissue organization, offers free color to all authors.
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