Journal of Histochemistry & Cytochemistry最新文献_第2页

Distinct Single-Cell Expression Pattern of the Soluble Epoxide Hydrolase and Cytochrome p450 Epoxygenases in Human and Mouse Small Intestinal Epithelia. 可溶性环氧化物水解酶和细胞色素p450环氧化酶在人和小鼠小肠上皮中不同的单细胞表达模式。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-01 Epub Date: 2025-12-25 DOI: 10.1369/00221554251403631

Leyu Sun, Amy Tang, Jie Liao, Guang-Yu Yang

{"title":"Distinct Single-Cell Expression Pattern of the Soluble Epoxide Hydrolase and Cytochrome p450 Epoxygenases in Human and Mouse Small Intestinal Epithelia.","authors":"Leyu Sun, Amy Tang, Jie Liao, Guang-Yu Yang","doi":"10.1369/00221554251403631","DOIUrl":"10.1369/00221554251403631","url":null,"abstract":"Lipid signaling molecules are essential for maintaining intestinal mucosal barrier homeostasis. Over 80% of polyunsaturated fatty acids (PUFAs) are metabolized through the cytochrome P450 epoxygenase (CYP)-soluble epoxide hydrolase (sEH) axis, generating bioactive epoxy and diol fatty acids. However, the expression patterns and functional roles of these enzymes in the intestinal epithelium remain poorly defined. To address this, we used RNAscope in situ hybridization and publicly available single-cell RNA sequencing (scRNAseq) datasets to map the cell-type-specific expression of PUFA-metabolizing enzymes in the small intestine. In humans, we identified three major epithelial expression patterns: (1) stem-cell-dominant (CYP2E1), (2) enterocyte-dominant (CYP1A1), and (3) widespread expression across epithelial subsets for key CYP epoxygenases (CYP2C8, 2C9, 2C18, 2C19, 2J2, 2S1), sEH, and additional CYPs (3A4, 4A11, 4F2, 4F3, 4F8, 4F12). In mice, five distinct expression patterns were found: (1) stem cell and transit-amplifying cells (CYP2E1), (2) transit-amplifying dominant (CYP4F18), (3) transit-amplifying and enterocytes (CYP2C44), (4) enterocyte-dominant (CYP1A1), and (5) broadly expressed across all epithelial clusters (sEH, CYP2B10, 2S1, 2C55, 4F13, 4F16). Furthermore, in a radiation-induced small-bowel injury-regeneration model, we observed dynamic changes in CYP expression patterns. These findings provide the first high-resolution single-cell atlas of CYP-sEH axis enzymes in the intestinal epithelium, offering key insights into their potential roles in epithelial differentiation, injury response, and mucosal barrier integrity. This foundational work enables future studies to define the biological functions and therapeutic relevance of PUFA-derived lipid mediators in intestinal health and disease.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"163-179"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12743005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145833985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming Growth Factor-β Drives Epithelial Mesenchymal Transition and Reduces Synthesis of Unelaborated O-GalNAc Glycans in Breast Cancer Cells. 转化生长因子-β在乳腺癌细胞中驱动上皮间质转化并减少未加工的O-GalNAc聚糖的合成

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-01 Epub Date: 2026-02-28 DOI: 10.1369/00221554261416994

Joanna Cull, Ryan C Pink, Priya Samuel, Susan A Brooks

{"title":"Transforming Growth Factor-β Drives Epithelial Mesenchymal Transition and Reduces Synthesis of Unelaborated O-GalNAc Glycans in Breast Cancer Cells.","authors":"Joanna Cull, Ryan C Pink, Priya Samuel, Susan A Brooks","doi":"10.1369/00221554261416994","DOIUrl":"10.1369/00221554261416994","url":null,"abstract":"SummaryAltered O-glycosylation of cancer cells is frequently associated with metastasis and poor prognosis. During metastasis, cells must also lose their epithelial characteristics and become mesenchymal and motile, termed epithelial-mesenchymal transition (EMT). While it is established that transforming growth factor beta-1 (TGF-β1) can induce EMT, the effect of cytokine-induced EMT on O-glycosylation of breast cancer cells has not previously been explored. MCF-7 and T47-D breast cancer cells were treated with TGF-β1 over a time course of up to 7 days. Morphological changes were assessed using confocal microscopy and quantified; levels of EMT marker expression were assessed using immunofluorescence with confocal microscopy and western blot. The effect of TGF-β1 on synthesis of unelaborated Tn antigen was explored using Helix pomatia agglutinin (HPA) labelling. TGF-β1 treatment induced morphological changes, resulting in an elongated, mesenchymal-like phenotype, and a reduction of epithelial marker E-cadherin but did not detectably induce mesenchymal markers N-cadherin or vimentin. It also resulted in a significant reduction in unelaborated Tn antigen, detected by HPA labelling. These observations are consistent with TGF-β1 inducing an 'early' or partial EMT state over this timeframe, and a concomitant change in O-glycosylation, consistent with the synthesis of more elaborated O-glycan structures; such glycoplasticity may function in metastasis. (J Histochem Cytochem XX:XXX-XXX, XXXX).","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"193-214"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12950527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147321758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Akkermansia muciniphila Selectively Reshapes Small Intestinal Cell Populations. 嗜粘杆菌选择性地重塑小肠细胞群。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-01 Epub Date: 2026-02-16 DOI: 10.1369/00221554261417734

Rachel Edens-Valentine, Kristen A Engevik, Melinda A Engevik, Amy C Engevik

{"title":"Akkermansia muciniphila Selectively Reshapes Small Intestinal Cell Populations.","authors":"Rachel Edens-Valentine, Kristen A Engevik, Melinda A Engevik, Amy C Engevik","doi":"10.1369/00221554261417734","DOIUrl":"10.1369/00221554261417734","url":null,"abstract":"The gastrointestinal tract harbors a dynamic microbial ecosystem that interfaces with the intestinal epithelium. Among this community, Akkermansia muciniphila, a mucin-degrading microbe, has garnered attention for its impact on gut health. While well studied in the colon, its influence on the small intestine remains underexplored. To examine direct effects of A. muciniphila, we used gnotobiotic mice. Germ-free mice were inoculated with Brain Heart Infusion (BHI) media or 10⁹ viable A. muciniphila in BHI. After 21 days, small intestinal tissue was collected. Fluorescence in situ hybridization confirmed A. muciniphila colonization. Immunofluorescence staining revealed increased epithelial cell proliferation, unchanged goblet cell numbers, but altered mucus composition with reduced fucose residues. Tuft cell numbers and group 2 innate lymphoid cells (ILC2s) were also elevated in A. muciniphila-colonized mice. Analysis of conditioned media and the A. muciniphila genome identified succinate production, a metabolite known to expand tuft cells. Thus, A. muciniphila alone is sufficient to increase tuft cells in the small intestine, potentially via succinate signaling. These findings reveal a novel role for A. muciniphila in regulating gastrointestinal homeostasis.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"215-233"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12913043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146202035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting CCNB1 Repressed Cartilage Degradation Induced by Inflammation Through NF-κB Pathway in Osteoarthritis. 靶向CCNB1通过NF-κB途径抑制炎症诱导的骨关节炎软骨降解。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-01 Epub Date: 2026-02-23 DOI: 10.1369/00221554251410643

Hua Li, Yuxue Qiao, Mengshuang Ding, Yirong Liu

{"title":"Targeting CCNB1 Repressed Cartilage Degradation Induced by Inflammation Through NF-κB Pathway in Osteoarthritis.","authors":"Hua Li, Yuxue Qiao, Mengshuang Ding, Yirong Liu","doi":"10.1369/00221554251410643","DOIUrl":"10.1369/00221554251410643","url":null,"abstract":"Inflammation contributes to osteoarthritis, and cyclin B1 (CCNB1) dysregulation is implicated. Understanding its role and regulation is crucial for developing targeted therapies against this degenerative disease. Mice chondrocytes were acquired from C57BL/6 wild-type mice and treated with interleukin (IL)-1β for inflammation induction, followed by assays for cell viability, apoptosis, inflammatory mediators, cartilage markers, and nuclear factor kappa B (NF-κB) pathway. CCNB1 was knocked down or upregulated in chondrocytes, respectively. The modified Hulth method was used to establish osteoarthritis model. The knee joint was visualized using micro-computed tomography, and histopathologic evaluation was carried out by immunohistochemistry staining, Safranin O/fast green, and hematoxylin and eosin staining for cartilage degradation markers. CCNB1 knockdown inhibited IL-1β-caused decrease in cell viability and increase in apoptosis of chondrocytes. Inflammatory mediators in IL-1β-treated chondrocytes were decreased after CCNB1 knockdown. CCNB1 knockdown reduced the expression of MMP-13 and ADAMTS-5, while elevated collagen II and aggrecan accumulation, alongside with NF-κB inactivation, in chondrocytes administered with IL-1β. Targeting inhibition of CCNB1 reduced the production of inflammation regulators, decreased cartilage degradation, and blocked NF-κB pathway activation. Targeting CCNB1 may serve as a potential therapeutic strategy for osteoarthritis by reducing inflammation, protecting cartilage, and modulating the NF-κB pathway.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"235-248"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12932133/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147275699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Expression of Histamine H₃ Receptor In Healthy, Preneoplastic, and Tumoral Pancreatic Tissue: A Pilot Study. 组胺H3受体在健康、肿瘤前和肿瘤胰腺组织中的差异表达：一项初步研究

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-01 Epub Date: 2026-01-01 DOI: 10.1369/00221554251410292

Daniela Speisky, Melisa B Nicoud, Alejandro Iotti, Karina Formoso, Paolo Lauretta, María Teresa García de Dávila, Vanina A Medina

{"title":"Differential Expression of Histamine H3 Receptor In Healthy, Preneoplastic, and Tumoral Pancreatic Tissue: A Pilot Study.","authors":"Daniela Speisky, Melisa B Nicoud, Alejandro Iotti, Karina Formoso, Paolo Lauretta, María Teresa García de Dávila, Vanina A Medina","doi":"10.1369/00221554251410292","DOIUrl":"10.1369/00221554251410292","url":null,"abstract":"Pancreatic ductal adenocarcinoma (PDAC) is one of the leading causes of cancer-related death worldwide, with a mortality rate almost equal to its incidence, highlighting the urgent need for novel biomarkers and therapeutic targets. This pilot study aims to investigate the potential value of histamine H3 receptor (H3R) as a prognostic biomarker for this lethal disease. We analyzed the H3R expression in PDAC using the RNA sequencing data set from The Cancer Genome Atlas (Pan-Cancer Atlas). In addition, H3R protein levels were evaluated by immunohistochemistry in 27 PDAC samples and compared with adjacent preneoplastic pancreatic tissue of the same patient, and with 10 non-related healthy pancreatic tissues. This preliminary study shows that the H3R is barely expressed in healthy tissue. Interestingly, H3R was detected in 96% of PDAC samples, and its expression in tumoral tissue was significantly higher when compared with its expression in preneoplastic tissue, and it was associated with a better prognosis in terms of overall survival. Present findings suggest that H3R may serve as a potential prognostic biomarker in PDAC. Future research aimed at elucidating the role of H3R in PDAC biology and its prognostic value in larger patient cohorts is warranted.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"181-191"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12759016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145889322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lamina Propria Collagen Architecture in Interstitial Cystitis/Bladder Pain Syndrome. 间质性膀胱炎/膀胱疼痛综合征的固有层胶原结构。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-01 Epub Date: 2026-01-17 DOI: 10.1369/00221554251410640

Hannah Ruetten, Raymond Xu, Harrison Martin, Dylan T Wolff, Wencheng Li, Kaylee Ferrara, L McKenna Huse, Robert J Evans, Gopal Badlani, Stephen J Walker

{"title":"Lamina Propria Collagen Architecture in Interstitial Cystitis/Bladder Pain Syndrome.","authors":"Hannah Ruetten, Raymond Xu, Harrison Martin, Dylan T Wolff, Wencheng Li, Kaylee Ferrara, L McKenna Huse, Robert J Evans, Gopal Badlani, Stephen J Walker","doi":"10.1369/00221554251410640","DOIUrl":"10.1369/00221554251410640","url":null,"abstract":"Interstitial cystitis/bladder pain syndrome (IC/BPS) is a heterogeneous condition of uncertain etiology. We assessed bladder collagen characteristics in phenotypically characterized IC/BPS patient subgroups that may influence pathophysiology. Forty-four females (30 IC/BPS; 14 non-IC/BPS) were included. Patients were divided into groups based on Hunner lesions (HL; N=14 or non-HL; N=16) and anesthetic bladder capacity (BC) (BC≤500 cc; low BC; N=17 or BC>500 cc; non-low BC; N=13). Bladder biopsy tissue slides were stained with hematoxylin and eosin or picrosirius red and semi-quantitatively analyzed by a pathologist. CT-FIRE software was used to quantitatively assess lamina propria collagen. All patients with IC/BPS had lower collagen fiber density independent of subgroup (p<0.0001-0.0038) compared to controls. HL had more peri-muscular collagen accumulation (p=0.0061), more acute inflammation (p=0.0364), more severe chronic inflammation (p=0.0088), and narrower collagen fibers than non-HL and controls. Non-low-BC patients had lower collagen density (p=0.0075) and straighter collagen fibers (p=0.0127) than low BC. Low-BC patients had narrower collagen fibers than control (p=0.0096). IC/BPS, regardless of subgroup, is associated with a bladder lamina propria with diminished collagen density. HL, non-low-BC, and low-BC subgroups have unique collagen characteristics. These findings suggest a collagen fiber destruction and redistribution process, which differs by subgroup, and may contribute to pathophysiology of IC/BPS.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"129-142"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12812059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145989531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes. 联合受激发射耗尽显微镜与荧光寿命成像显微镜研究肾小球足细胞中TIE2蛋白。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-03-01 Epub Date: 2026-01-21 DOI: 10.1369/00221554251413643

Iulia-Aneta Dénes, Irina A Okkelman, Herlinde De Keersmaecker, Pieter Cornillie, Ruslan I Dmitriev, Ward De Spiegelaere

{"title":"Correlating Stimulated Emission Depletion Microscopy With Fluorescence Lifetime Imaging Microscopy to Study the TIE2 Protein on Kidney Glomerular Podocytes.","authors":"Iulia-Aneta Dénes, Irina A Okkelman, Herlinde De Keersmaecker, Pieter Cornillie, Ruslan I Dmitriev, Ward De Spiegelaere","doi":"10.1369/00221554251413643","DOIUrl":"10.1369/00221554251413643","url":null,"abstract":"The TIE2 transmembrane receptor protein, known for its role in vascular stability and blood vessel remodeling, has primarily been studied in endothelial cells. This receptor has also been found on several non-endothelial cell types including podocytes, although its presence on podocytes remains a matter of debate. Conventional immunofluorescence approaches applied to membrane proteins are often challenged by the strong tissue autofluorescence spanning green and red parts of the electromagnetic spectrum, sample thickness, and the antibody specificity. Here, we used stimulated emission depletion (STED) microscopy, a super-resolution microscopy method, to detect the TIE2 protein in complex biological tissue with a resolution of less than 50 nm. To further confirm the presence of TIE2 on podocytes and to investigate its localization, we used fluorescence lifetime imaging microscopy (FLIM) to more effectively distinguish between nonspecific autofluorescence and specific emission in cleared and antibody-labeled tissue samples. By correlating these two techniques, we mapped the subcellular localization of TIE2 on mouse podocytes and confirmed its presence on the cell surface facing the Bowman's space, albeit at lower expression levels. This study highlights the potential complementary power of STED and FLIM methods and provides additional evidence that the TIE2 receptor is present on podocytes.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"143-161"},"PeriodicalIF":1.5,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12823351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146010745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and Diagnostic Significance of Clinical Routine VMAT2 Immunostaining in Neuroendocrine Neoplasia: A Study of Institutional Cases. 临床常规VMAT2免疫染色在神经内分泌肿瘤中的表达及诊断意义：一项机构病例研究。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-02-23 DOI: 10.1369/00221554261424958

Zandra Ankers, L Samuel Hellgren, C Christofer Juhlin

{"title":"Expression and Diagnostic Significance of Clinical Routine VMAT2 Immunostaining in Neuroendocrine Neoplasia: A Study of Institutional Cases.","authors":"Zandra Ankers, L Samuel Hellgren, C Christofer Juhlin","doi":"10.1369/00221554261424958","DOIUrl":"10.1369/00221554261424958","url":null,"abstract":"Vesicular monoamine transporter 2 (VMAT2) is an integral membrane protein that packages monoamines, including dopamine, norepinephrine, serotonin, and histamine, into synaptic vesicles. VMAT2 is normally expressed in sympathetic, enteric, and central nervous system neurons and in neuroendocrine cells. Although VMAT2 immunoreactivity was originally reported in a wide range of neuroendocrine neoplasms (NENs), its diagnostic value has been largely overlooked in recent practice due to reliance on established markers such as chromogranin A (CgA), synaptophysin (SYP), and the transcription factors ISLET1 and INSM1. To reassess the relevance of VMAT2, we evaluated its expression relative to CgA, SYP, ISLET1, and INSM1 in 60 consecutively diagnosed NENs from diverse anatomic sites and tumor grades. VMAT2 was positive in 37 of 39 (95%) well-differentiated neuroendocrine tumors (NETs; grades 1-3), in all adrenal paragangliomas (7 of 7) and in one medullary thyroid carcinoma, showing sensitivity comparable to CgA and INSM1 and slightly lower than SYP. VMAT2 was detected in 7 of 12 (58%) neuroendocrine carcinomas. Sensitivity was highest in small-intestinal and gastric NETs and more variable in pancreatic NETs. These results support VMAT2 as a robust, differentiation-linked neuroendocrine marker whose inclusion in modern immunohistochemical panels may improve diagnostic accuracy.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261424958"},"PeriodicalIF":1.5,"publicationDate":"2026-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12932129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147275729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Brain Tissue Preservation for Nucleic Acid Stability. 核酸稳定性的脑组织保存优化。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-01-01 Epub Date: 2026-01-05 DOI: 10.1369/00221554251400421

Mario Novelli, Caine C Smith, Dhiraj Maskey, Julia Stevens, Marina Gimeno, Neil Horadagoda, Greg T Sutherland

{"title":"Optimization of Brain Tissue Preservation for Nucleic Acid Stability.","authors":"Mario Novelli, Caine C Smith, Dhiraj Maskey, Julia Stevens, Marina Gimeno, Neil Horadagoda, Greg T Sutherland","doi":"10.1369/00221554251400421","DOIUrl":"10.1369/00221554251400421","url":null,"abstract":"Postmortem human brain tissue is an important resource for brain research. Spatial transcriptomics is a novel technology that utilizes formalin-fixed, paraffin-embedded (FFPE) or cryosections, with the former having good cytoarchitecture but poor RNA quality and vice versa for frozen tissue. Sheep brains (n=16) were used to test various protocols that simulate the conditions around the preparation and dissemination of human postmortem FFPE and frozen brain tissue to optimize for future spatial transcriptomic work. FFPE and frozen tissues were investigated for RNA quality, while hematoxylin and eosin-stained cryosections were analyzed by quantifying tissue voids as a proxy for cytoarchitectural integrity. Postmortem interval reduced the RNA integrity number equivalent (RINe) of frozen tissue from 7.2 (24 hr) to 4.8 (168 hr). In FFPE tissue, the percentage of RNA fragments greater than 200 nucleotides (DV200) values ranged from 18.9% to 69.01%, with the highest values observed in samples fixed for less than 24 hr. Pretreatment with liquid nitrogen before -80°C storage resulted in the lowest voids (11.6%) in cryosections, but cryoprotectants had little effect. These findings provide researchers with guidelines for tissue preparation in spatial transcriptomics. However, freezing protocols require further refinement to approach the cytoarchitecture of FFPE tissue.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"101-117"},"PeriodicalIF":1.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12774817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145900649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of Tetraspanin 4 Relative to Therapy-induced Senescence Markers in Breast Cancer in Response to Neoadjuvant Chemotherapy. 乳腺癌对新辅助化疗的反应中与治疗诱导的衰老标志物相关的Tetraspanin 4的表达

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-01-01 Epub Date: 2025-11-20 DOI: 10.1369/00221554251391226

Tareq Saleh, Sofian Al Shboul, Kholoud Friehat, Nebras Melhem, Alia Al Mohtaseb, Sura B AlOmari, Mohammad Matalka, Nisreen Abu Shahin, Shrouq Daromar, Ahmad Alhesa, Mohammad El-Sadoni, Randa G Naffa, Moureq R Alotaibi, Jack Brydon, Ted Hupp, Jakub Faktor, Kenneth Weke, Sachin Kote, AbdelKader Battah

{"title":"Expression of Tetraspanin 4 Relative to Therapy-induced Senescence Markers in Breast Cancer in Response to Neoadjuvant Chemotherapy.","authors":"Tareq Saleh, Sofian Al Shboul, Kholoud Friehat, Nebras Melhem, Alia Al Mohtaseb, Sura B AlOmari, Mohammad Matalka, Nisreen Abu Shahin, Shrouq Daromar, Ahmad Alhesa, Mohammad El-Sadoni, Randa G Naffa, Moureq R Alotaibi, Jack Brydon, Ted Hupp, Jakub Faktor, Kenneth Weke, Sachin Kote, AbdelKader Battah","doi":"10.1369/00221554251391226","DOIUrl":"10.1369/00221554251391226","url":null,"abstract":"Therapy-induced senescence (TIS) is a component of breast cancer (BC) treatment. Tetraspanins have emerging roles in cancer biology. Tetraspanin 4 (TSPAN4 [NAG2]) has been implicated in tumor progression, however, its association with TIS remains unexplored. We investigated TSPAN4 expression in BC samples from patients who received neoadjuvant chemotherapy (NAC) and its association with TIS markers. Thirty-eight paired pre- and post-NAC BC samples were analyzed using immunohistochemistry (IHC) staining for TSPAN4 and TIS-associated biomarkers (Lamin B1 and Ki67). Pairwise analysis of senescence-related gene expression (LMNB1, MKI67, CDKN1A, ATM, IGFBP7, MMP2, CXCL14, and CCL5) was performed in an independent geneset of 68 paired pre- and post-NAC BC patient samples. NAC reduced the expression of senescence-associated proliferation markers Ki67 and Lamin B1 in BC samples, with 84% and 76% of patients showing decreased expression, respectively (p<0.001). Senescence-associated gene expression analysis revealed consistent upregulation of CDKN1A, ATM, IGFBP7, MMP2, CXCL14, and CCL5 post-NAC (p<0.001), while LMNB1 and MKI67 were significantly downregulated (p<0.0001 and p=0.007, respectively). A subset (15/38; 39%) of samples demonstrated upregulation of the TSPAN4 expression post-NAC (p<0.01). NAG2 was upregulated in 54/68 patients post-NAC (p<0.00001) and its expression correlated positively with senescence-associated genes. An association between TSPAN4 and TIS post-NAC was identified.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"19-37"},"PeriodicalIF":1.5,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12638236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145563889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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