Journal of Histochemistry & Cytochemistry最新文献_第2页

Human iPSC-Derived Blood Vessel Organoids for Studying Chronic Hypoxia-Induced Microvascular Dysfunction. 人类ipsc衍生血管类器官用于研究慢性缺氧诱导的微血管功能障碍。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-07-01 Epub Date: 2026-05-11 DOI: 10.1369/00221554261437861

Paola Serrano Martinez, Maxime Cammeraat, Amber Teppema, Cornelis J F van Noorden, Reinier O Schlingemann, Ingeborg Klaassen

{"title":"Human iPSC-Derived Blood Vessel Organoids for Studying Chronic Hypoxia-Induced Microvascular Dysfunction.","authors":"Paola Serrano Martinez, Maxime Cammeraat, Amber Teppema, Cornelis J F van Noorden, Reinier O Schlingemann, Ingeborg Klaassen","doi":"10.1369/00221554261437861","DOIUrl":"10.1369/00221554261437861","url":null,"abstract":"Microvascular dysfunction due to hypoxia is a key contributor in the pathogenesis of many disorders including cancer and retinal and cardiovascular diseases, but relevant human models are missing. Here, we present a robust 3D in vitro method with the use of human induced pluripotent stem cell-derived blood vessel organoids to analyze in vitro microvascular remodeling. We present a detailed practical pipeline combining optical tissue clearing, high-resolution immunofluorescence, and surface marker analysis to quantitatively assess hypoxia-driven changes in endothelial cells, pericytes, and the basal lamina. Exposure of these blood vessel organoids to chronic hypoxia (1% O2) for 1 week recapitulated key pathological features, including structural remodeling and a dysregulated secretome with altered vascular endothelial growth factor signaling. This approach establishes a versatile and human-relevant platform to study microvascular remodeling induced by chronic hypoxia and other pathological stimuli and their contribution to microvascular-related diseases.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"423-434"},"PeriodicalIF":1.5,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13161033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147868489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

P63 and Tight Junctions Targeted for Therapy in Human Salivary Duct Adenocarcinoma Cells. P63和紧密连接靶向治疗人唾液管腺癌细胞。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-07-01 Epub Date: 2026-05-19 DOI: 10.1369/00221554261435677

Masahiko Oyanagi, Takumi Konno, Takayuki Kohno, Akito Kakiuchi, Shin Kikuchi, Terufumi Kubo, Soshi Nishida, Masaya Nakano, Kizuku Ohwada, Takuya Kakuki, Kazufumi Obata, Makoto Kurose, Ikuto Kimura, Kosuke Suzuki, Motoki Tanaka, Yuki Ohsaki, Kenichi Takano, Takashi Kojima

{"title":"P63 and Tight Junctions Targeted for Therapy in Human Salivary Duct Adenocarcinoma Cells.","authors":"Masahiko Oyanagi, Takumi Konno, Takayuki Kohno, Akito Kakiuchi, Shin Kikuchi, Terufumi Kubo, Soshi Nishida, Masaya Nakano, Kizuku Ohwada, Takuya Kakuki, Kazufumi Obata, Makoto Kurose, Ikuto Kimura, Kosuke Suzuki, Motoki Tanaka, Yuki Ohsaki, Kenichi Takano, Takashi Kojima","doi":"10.1369/00221554261435677","DOIUrl":"10.1369/00221554261435677","url":null,"abstract":"Salivary gland cancer is a rare heterogeneous group of neoplasms with complex histopathologic patterns, including carcinoma with squamous differentiation, for which surgery is the standard treatment. However, further research is essential for developing new therapies. Head and neck squamous cell carcinoma is positive for p63 and negative for the bicellular tight junction protein cingulin (CGN). p63 plays a key role in cancer progression such as cell proliferation, migration, apoptosis, and squamous differentiation. To understand the roles of p63 in salivary duct adenocarcinoma, we investigated the malignancy by using overexpression of deltaNp63 in p63-negative salivary duct adenocarcinoma cells PGC2E derived from parotid gland duct adenocarcinoma. By transfection with deltaNp63, the PGC2E cells exhibited increased nuclear p63 expression and reduced CGN levels at the membrane. Overexpression of deltaNp63 disrupted epithelial polarity and epithelial permeability barriers, promoted cell proliferation and migration, and enhanced cellular metabolism. Treatment with inhibitors of histone deacetylase and nuclear factor kappa B, and antibodies to tumor necrosis factor-α and tricellular tight junction protein lipolysis-stimulated lipoprotein receptor induced apoptosis in PGC2E cells. However, overexpression of deltaNp63 prevented the induced apoptosis. These findings suggest that p63 contributes to cancer malignancy and the complex phenotype, and it may be possible to develop a novel treatment via p63.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"385-404"},"PeriodicalIF":1.5,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13186921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147973044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Manual to Macro: A Reproducible Fiji Workflow for Semi-automated Collagen Fibril Diameter Quantification in Transmission Electron Microscopy. 从手动到宏：可复制的斐济工作流程半自动胶原纤维直径定量在透射电子显微镜。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-07-01 Epub Date: 2026-05-05 DOI: 10.1369/00221554261440588

Apoorva Bhandari, Nobuyo Mizuno, Sara F Tufa, Lynn Y Sakai, Douglas R Keene, Sherene Shalhub

{"title":"From Manual to Macro: A Reproducible Fiji Workflow for Semi-automated Collagen Fibril Diameter Quantification in Transmission Electron Microscopy.","authors":"Apoorva Bhandari, Nobuyo Mizuno, Sara F Tufa, Lynn Y Sakai, Douglas R Keene, Sherene Shalhub","doi":"10.1369/00221554261440588","DOIUrl":"10.1369/00221554261440588","url":null,"abstract":"Quantitative assessment of collagen fibril diameter is essential for understanding ultrastructural changes in aging, connective tissue disorders, and extracellular matrix remodeling. Although transmission electron microscopy (TEM) is widely used for this purpose, existing methods for fibril measurement are predominantly manual, operator-dependent, and prone to inconsistent reporting. To address this, we developed a semi-automated Fiji/ImageJ (IJ1) macro that standardizes fibril diameter measurements from two-dimensional TEMs. The macro uses a wand-based region-of-interest (ROI) detection strategy with integrated geometric validation. It calculates multiple metrics, including area-equivalent diameters, ellipse-derived major and minor axes, shape consistency indices, and spatial localization across the image field. Built-in quality assurance thresholds exclude oblique or irregular profiles, ensuring accurate identification of true fibril cross-sections. Real-time visual overlays support live validation and user feedback during analysis. We detail the implementation, analytical workflow, and validation approach, provide practical guidance for reproducible use across operators and datasets, and show proof-of-concept utility for analysis of collagen fibrils in vascular Ehlers-Danlos syndrome (VEDS) subject dermal samples. This open-source, extensible tool enhances standardization and reproducibility in collagen fibril morphometry for ultrastructural research.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"449-462"},"PeriodicalIF":1.5,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13149348/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Glial Cell Line-Derived Neurotrophic Factor and Ciliary Neurotrophic Factor on Extraocular and Limb Muscle Precursor Cells. 神经胶质细胞系源性神经营养因子和睫状体神经营养因子对眼外和肢体肌前体细胞的影响。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-07-01 Epub Date: 2026-04-13 DOI: 10.1369/00221554261435666

Austin J Winker, Laura L Johnson, Linda K McLoon

{"title":"Effects of Glial Cell Line-Derived Neurotrophic Factor and Ciliary Neurotrophic Factor on Extraocular and Limb Muscle Precursor Cells.","authors":"Austin J Winker, Laura L Johnson, Linda K McLoon","doi":"10.1369/00221554261435666","DOIUrl":"10.1369/00221554261435666","url":null,"abstract":"SummaryMyogenic precursor cells within skeletal muscles are responsible for the maintenance of skeletal muscle over a lifetime. Neurotrophic and growth factors play critical roles in this maintenance and in responses of myogenic precursor cells. Both glial cell line-derived neurotrophic factor (GDNF) and ciliary neurotrophic factor (CNTF) play roles in the maintenance and/or development of strabismus, yet few studies have examined their roles in the control of myogenic precursor cell proliferation and differentiation. Two populations of myogenic precursor cells were isolated from extraocular and leg muscle by fluorescence-activated cell sorting: EECD34 cells, largely PITX2-positive, and PAX7-positive cells. Cultures were treated with GDNF or CNTF and processed immunohistochemically to determine proliferation and differentiation rates. Neither GDNF nor CNTF affected cell proliferation rates for either muscle. Both treatments impacted cell differentiation by increasing multinucleated cell number, with TA-derived precursor cells producing cells containing large numbers of nuclei and EOM-derived precursor cells producing shorter multinucleated fibers with fewer nuclei. These differences may explain the presence of extremely short myofibers within normal adult EOM compared with limb muscle. As GDNF and CNTF are downregulated in strabismic muscles, data suggest that myofiber length homeostasis may be disrupted in strabismic EOM and suggest possible approaches for strabismus treatment.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"435-448"},"PeriodicalIF":1.5,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13076467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147674202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ADAMTS1 Is Required for Ventral Abdominal Wall Closure. 腹腹壁闭合需要ADAMTS1。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-07-01 Epub Date: 2026-04-28 DOI: 10.1369/00221554261438169

Gabriel Opoku, Kentaro Ikemura, Omer F Hatipoglu, Takashi Ohtsuki, Ikumi Sato, Hibiki Taniguchi, Shino Sakamoto, Farhana Hasib, Shogo Watanabe, Timothy J Mead, Suneel S Apte, Satoshi Hirohata

{"title":"ADAMTS1 Is Required for Ventral Abdominal Wall Closure.","authors":"Gabriel Opoku, Kentaro Ikemura, Omer F Hatipoglu, Takashi Ohtsuki, Ikumi Sato, Hibiki Taniguchi, Shino Sakamoto, Farhana Hasib, Shogo Watanabe, Timothy J Mead, Suneel S Apte, Satoshi Hirohata","doi":"10.1369/00221554261438169","DOIUrl":"10.1369/00221554261438169","url":null,"abstract":"ADAMTS1 (a disintegrin-like and metalloproteinase domain with thrombospondin type 1 repeats) is a secreted metalloproteinase with a known role in extracellular matrix remodeling in cardiovascular development and female fertility. Because of conflicting report of embryonic lethality and survival in Adamts1 mutant mice, we report generation and characterization of a new knockout (KO) allele, which led to detection of neonatal lethal omphalocele (persistent umbilical hernia) as a new Adamts1-dependent birth defect. This phenotype was also detected in another mutants with an in-frame lacZ insertion. β-galactosidase staining in the latter showed that Adamts1 was strongly expressed in the undifferentiated cells in the developing ventral abdominal wall preceding midline fusion at the umbilical cord attachment site. The omphalocele was characterized by the accumulation of the proteoglycan versican along with reduced proteolysis and impaired development of the superficial muscle layer, the panniculus carnosus, around the site of umbilical cord attachment. Furthermore, we observed sustained expression of the transcription factor, paired-like homeodomain transcription factor 2 in KO embryos, whereas it was downregulated in wild-type embryos during late embryogenesis. ADAMTS1 is thus a new matrisome component required for body wall closure.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"405-421"},"PeriodicalIF":1.5,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seeing, Interpreting, and Trusting Images in the Age of Artificial Intelligence. 人工智能时代的视觉、解读和信任图像。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-06-08 DOI: 10.1369/00221554261454582

Katia Cortese, Patrizio Castagnola

{"title":"Seeing, Interpreting, and Trusting Images in the Age of Artificial Intelligence.","authors":"Katia Cortese, Patrizio Castagnola","doi":"10.1369/00221554261454582","DOIUrl":"10.1369/00221554261454582","url":null,"abstract":"Artificial intelligence (AI) is transforming how cell biologists generate, analyze, and interpret visual data. Automated pipelines and large-scale image analysis increase throughput and reproducibility, yet they also modify how visual evidence is validated and trusted. This Perspective examines the conceptual implications of this shift, arguing that the integration of AI into imaging demands rather than replaces human morphological expertise. We distinguish between three conceptually distinct phenomena: (1) automation of acquisition, (2) AI-driven interpretation, and (3) the emergence of synthetic images generated without experimental basis. Through examples ranging from classical morphological misinterpretations to the rise of generative models, we show how both human and algorithmic systems can distort meaning when interpretation is detached from context. While AI can match or surpass human precision, metavisual competence remains essential to distinguish signals from artifacts. The current challenge is not technological but scientific: to ensure that computational power and human insight co-evolve toward reliable visual knowledge. We call for a renewed education in critical visual literacy, formal recognition of imaging specialists, and transparent standards of image provenance, such as the publication of raw instrument metadata. Preserving interpretative competence is crucial for the integrity of visual evidence in biomedical science.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"221554261454582"},"PeriodicalIF":1.5,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13249602/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148204847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression and Diagnostic Significance of Clinical Routine VMAT2 Immunostaining in Neuroendocrine Neoplasia: A Study of Institutional Cases. 临床常规VMAT2免疫染色在神经内分泌肿瘤中的表达及诊断意义：一项机构病例研究。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-05-01 Epub Date: 2026-02-23 DOI: 10.1369/00221554261424958

Zandra Ankers, L Samuel Hellgren, C Christofer Juhlin

{"title":"Expression and Diagnostic Significance of Clinical Routine VMAT2 Immunostaining in Neuroendocrine Neoplasia: A Study of Institutional Cases.","authors":"Zandra Ankers, L Samuel Hellgren, C Christofer Juhlin","doi":"10.1369/00221554261424958","DOIUrl":"10.1369/00221554261424958","url":null,"abstract":"Vesicular monoamine transporter 2 (VMAT2) is an integral membrane protein that packages monoamines, including dopamine, norepinephrine, serotonin, and histamine, into synaptic vesicles. VMAT2 is normally expressed in sympathetic, enteric, and central nervous system neurons and in neuroendocrine cells. Although VMAT2 immunoreactivity was originally reported in a wide range of neuroendocrine neoplasms (NENs), its diagnostic value has been largely overlooked in recent practice due to reliance on established markers such as chromogranin A (CgA), synaptophysin (SYP), and the transcription factors ISLET1 and INSM1. To reassess the relevance of VMAT2, we evaluated its expression relative to CgA, SYP, ISLET1, and INSM1 in 60 consecutively diagnosed NENs from diverse anatomic sites and tumor grades. VMAT2 was positive in 37 of 39 (95%) well-differentiated neuroendocrine tumors (NETs; grades 1-3), in all adrenal paragangliomas (7 of 7) and in one medullary thyroid carcinoma, showing sensitivity comparable to CgA and INSM1 and slightly lower than SYP. VMAT2 was detected in 7 of 12 (58%) neuroendocrine carcinomas. Sensitivity was highest in small-intestinal and gastric NETs and more variable in pancreatic NETs. These results support VMAT2 as a robust, differentiation-linked neuroendocrine marker whose inclusion in modern immunohistochemical panels may improve diagnostic accuracy.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"257-266"},"PeriodicalIF":1.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12932129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147275729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Type and Size of Fluorescent Proteins Modulate the Localization of PTEN and Its Fragments, but Have Little or No Effect on Mutant PTEN or Stressed Cells. 荧光蛋白的类型和大小调节PTEN及其片段的定位，但对突变PTEN或应激细胞的影响很小或没有影响。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-05-01 Epub Date: 2026-03-27 DOI: 10.1369/00221554261434309

Takashi Kato, Yuuri Kawakami

{"title":"Type and Size of Fluorescent Proteins Modulate the Localization of PTEN and Its Fragments, but Have Little or No Effect on Mutant PTEN or Stressed Cells.","authors":"Takashi Kato, Yuuri Kawakami","doi":"10.1369/00221554261434309","DOIUrl":"10.1369/00221554261434309","url":null,"abstract":"The functions of phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor suppressor, depend on its subcellular localization. At the plasma membrane, PTEN dephosphorylates phosphatidylinositol-3,4,5-triphosphate to inhibit AKT signaling, whereas nuclear PTEN contributes to the maintenance of genomic stability. Fluorescent proteins (FPs) are widely used to assess PTEN's subcellular localization; however, both the intrinsic properties of FPs (e.g., molecular size) and the choice of FP can influence subcellular localization. This study aimed to determine whether FP fusion affects the subcellular localization of PTEN and its mutant forms under conditions involving DNA damage. mCherry typically promotes cytosolic localization of FP-fused PTEN, indicating that FP selection may affect the interpretation of localization data. Furthermore, FP fusion increases the molecular size of the truncated PTEN fragment, which may impede its nuclear import. In comparison, PTEN mutants such as PTENK13R or PTENA4, which predominantly localize to the cytoplasm or nucleus, respectively, show a minimal dependence on the type of FP. Similarly, DNA damage-induced nuclear accumulation of PTEN appears to be independent of the FP type. These findings underscore the importance of carefully considering the effects of FP fusion when investigating the mechanisms regulating the nuclear translocation of PTEN.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"311-320"},"PeriodicalIF":1.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13033038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147530043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Chloride Channel Regulator, Calcium-Activated-1 Is Expressed in Synoviocytes and Articular Chondrocytes in Health and Disease. 健康和疾病中滑膜细胞和关节软骨细胞表达的氯离子通道调节剂钙活化-1

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-05-01 Epub Date: 2026-03-15 DOI: 10.1369/00221554261423720

Judith Bushe, Jenny Fürstenau, Florian Bartenschlager, Simon Dökel, Vladimir M Jovanovic, Gundula Rösch, Zsuzsa Jenei-Lanzl, Frank Zaucke, Kristina Dietert, Achim D Gruber

{"title":"The Chloride Channel Regulator, Calcium-Activated-1 Is Expressed in Synoviocytes and Articular Chondrocytes in Health and Disease.","authors":"Judith Bushe, Jenny Fürstenau, Florian Bartenschlager, Simon Dökel, Vladimir M Jovanovic, Gundula Rösch, Zsuzsa Jenei-Lanzl, Frank Zaucke, Kristina Dietert, Achim D Gruber","doi":"10.1369/00221554261423720","DOIUrl":"10.1369/00221554261423720","url":null,"abstract":"The chloride channel regulator, calcium-activated-1, CLCA1, acts as a multifunctional secreted glycoprotein in anion channel modulation, mucus homeostasis, immune regulation, and other functions. So far, it has been described in goblet and other mucus-producing cells in mucous membranes, primarily of the respiratory, alimentary, and urogenital tracts. Here, we identify the expression of CLCA1 in fibroblast-like synoviocytes and superficial as well as, to a lesser extent, intermediate zone chondrocytes of diarthrodial articular joints. The expression pattern was found to be conserved in major joints in mice, pigs, and humans. First analyses of degenerate or infectious inflammatory joint conditions in mice or pigs, respectively, suggest rather continuous expression levels in articular disease. We speculate that CLCA1 may be involved in articular anion conductance, proteoglycan homeostasis, and inflammation of articular joints, possibly similar to analogous functions of this molecule as established in mucous membranes of the lungs and intestine.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"321-337"},"PeriodicalIF":1.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12989442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147463379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-step Gelatin Embedding Preserves Fibrocartilage Morphology, Enthesis, and Cartilage Stain of Bone Joints. 两步明胶包埋可保留骨关节的纤维软骨形态、骨骺和软骨染色。

IF 1.5 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2026-05-01 Epub Date: 2026-03-10 DOI: 10.1369/00221554261422633

Rafael Correia Cavalcante, Tongqing Zhou, Nidhi Bhandari, Zachary Rickman, Peter X Ma, Yuji Mishina

{"title":"Two-step Gelatin Embedding Preserves Fibrocartilage Morphology, Enthesis, and Cartilage Stain of Bone Joints.","authors":"Rafael Correia Cavalcante, Tongqing Zhou, Nidhi Bhandari, Zachary Rickman, Peter X Ma, Yuji Mishina","doi":"10.1369/00221554261422633","DOIUrl":"10.1369/00221554261422633","url":null,"abstract":"Maintaining the native morphology and characteristic staining patterns of dissected tissues is critical for histological analysis. Reliable preservation enables accurate assessment of structural integrity and cellular components, which is fundamental to identifying viable avenues for tissue regeneration and improving clinical outcomes. Acknowledging the constraints of conventional paraffin embedding, we propose an alternative method employing gelatin as an embedding medium. Gelatin is a natural, water-soluble protein derived from collagen, known for its biocompatibility. As a hydrogel, it provides a supportive matrix that closely mimics the natural extracellular matrix of soft tissues facilitating excellent preservation of structural and molecular features during the histological process. Here we demonstrate that this strategy offers superior support for fragile tissues, including fibrocartilage and hyaline cartilage across various anatomical sites, such as the temporomandibular joint, the knee joint, the long bone growth plate, and the intervertebral disc. To assess the maintenance of tissue morphology and matrix composition, histological sections were subjected to various staining techniques, including hematoxylin and eosin, Masson's trichome, safranin O, immunofluorescence, and von Kossa staining. Gelatin embedding resulted in superior maintenance of fibrocartilage architecture, as evidenced by histological evaluation. In addition, quantification of safranin O staining showed significantly greater glycosaminoglycan content in gelatin-embedded samples.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":" ","pages":"339-358"},"PeriodicalIF":1.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12975546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147433409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0