Journal of Histochemistry & Cytochemistry最新文献_第10页

Effect of Antigen Retrieval on Genomic DNA From Immunodissected Samples. 抗原提取对免疫解剖标本基因组DNA的影响。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2022-09-01 DOI: 10.1369/00221554221124163

Donald J Johann, Ik Jae Shin, Adam Roberge, Sarah Laun, Erich A Peterson, Meei Liu, Matthew A Steliga, Jason Muesse, Michael R Emmert-Buck, Michael A Tangrea

{"title":"Effect of Antigen Retrieval on Genomic DNA From Immunodissected Samples.","authors":"Donald J Johann, Ik Jae Shin, Adam Roberge, Sarah Laun, Erich A Peterson, Meei Liu, Matthew A Steliga, Jason Muesse, Michael R Emmert-Buck, Michael A Tangrea","doi":"10.1369/00221554221124163","DOIUrl":"https://doi.org/10.1369/00221554221124163","url":null,"abstract":"Immunohistochemical (IHC) staining is an established technique for visualizing proteins in tissue sections for research studies and clinical applications. IHC is increasingly used as a targeting strategy for procurement of labeled cells via tissue microdissection, including immunodissection, computer-aided laser dissection (CALD), expression microdissection (xMD), and other techniques. The initial antigen retrieval (AR) process increases epitope availability and improves staining characteristics; however, the procedure can damage DNA. To better understand the effects of AR on DNA quality and quantity in immunodissected samples, both clinical specimens (KRAS gene mutation positive cases) and model system samples (lung cancer patient-derived xenograft tissue) were subjected to commonly employed AR methods (heat induced epitope retrieval [HIER], protease digestion) and the effects on DNA were assessed by Qubit, fragment analysis, quantitative PCR, digital droplet PCR (ddPCR), library preparation, and targeted sequencing. The data showed that HIER resulted in optimal IHC staining characteristics, but induced significant damage to DNA, producing extensive fragmentation and decreased overall yields. However, neither of the AR methods combined with IHC prevented ddPCR amplification of small amplicons and gene mutations were successfully identified from immunodissected clinical samples. The results indicate for the first time that DNA recovered from immunostained slides after standard AR and IHC processing can be successfully employed for genomic mutation analysis via ddPCR and next-generation sequencing (NGS) short-read methods.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9527476/pdf/10.1369_00221554221124163.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10134250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuft Cells Are Present in Submandibular Glands Across Species. 不同物种的下颌下腺中都存在簇细胞

IF 1.9 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2022-09-01 Epub Date: 2022-08-21 DOI: 10.1369/00221554221120301

Harim Tavares Dos Santos, Kihoon Nam, Frank M Maslow, Travis Small, Tabitha L I Galloway, Laura M Dooley, Patrick T Tassone, Robert P Zitsch, Gary A Weisman, Olga J Baker

引用次数: 0

Human Pulmonary Vein Myocardial Sleeve Autonomic Neural Density and Cardiovascular Mortality. 人肺静脉心肌袖自主神经密度与心血管死亡率。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2022-09-01 DOI: 10.1369/00221554221129899

Denis Depes, Ari Mennander, Rauha Vehniäinen, Timo Paavonen, Ivana Kholová

{"title":"Human Pulmonary Vein Myocardial Sleeve Autonomic Neural Density and Cardiovascular Mortality.","authors":"Denis Depes, Ari Mennander, Rauha Vehniäinen, Timo Paavonen, Ivana Kholová","doi":"10.1369/00221554221129899","DOIUrl":"https://doi.org/10.1369/00221554221129899","url":null,"abstract":"Myocardial sleeves around pulmonary veins (PVs) are highly innervated structures with heterogeneous morphological and electrophysiological characteristics. Autonomic nerve dysfunction in the myocardium may be associated with an increased risk of cardiovascular morbidity and mortality. This article studied autonomic neural remodeling in myocardial sleeves around PVs and atrial-PV ostia with immunohistochemical and morphometric methods with clinicopathological correlations. PVs were collected from 37 and atrial-PV ostia from 17 human autopsy hearts. Immunohistochemical analysis was performed using antibodies against tyrosine hydroxylase (TH), choline acetyltransferase (CHAT), and growth-associated protein 43 (GAP43). In the PV cohort, subjects with immediate cardiovascular cause of death had significantly decreased sympathetic nerve density in fibro-fatty tissue vs those with non-cardiovascular cause of death (1624.53 vs 2522.05 µm2/mm2, p=0.038). In the atrial-PV ostia cohort, parasympathetic nerve density in myocardial sleeves was significantly increased in subjects with underlying cardiovascular cause of death (19.48 µm2/mm2) than subjects with underlying non-cardiovascular cause of death with no parasympathetic nerves detected (p=0.034). Neural growth regionally varied in sympathetic nerves and was present in most of the parasympathetic nerves. Heterogeneous autonomic nerve distribution and growth around PVs and atrial-PV ostia might play a role in cardiovascular morbidity and mortality. No association in nerve density was found with atrial fibrillation.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9527475/pdf/10.1369_00221554221129899.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10124337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatially Resolved and Highly Multiplexed Protein and RNA In Situ Detection by Combining CODEX With RNAscope In Situ Hybridization. 结合CODEX和RNAscope原位杂交技术进行空间分辨和高度复用的蛋白质和RNA原位检测。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2022-08-01 DOI: 10.1369/00221554221114174

Yilun Cheng, Rachel K Burrack, Qingsheng Li

引用次数: 4

Quantitative Assessment of the Apical and Basolateral Membrane Expression of VEGFR2 and NRP2 in VEGF-A-stimulated Cultured Human Umbilical Vein Endothelial Cells. vegf - a刺激培养的人脐静脉内皮细胞顶端和底侧膜中VEGFR2和NRP2表达的定量评价

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2022-08-01 DOI: 10.1369/00221554221115767

Esmeralda K Bosma, Shahan Darwesh, Jia Y Zheng, Cornelis J F van Noorden, Reinier O Schlingemann, Ingeborg Klaassen

{"title":"Quantitative Assessment of the Apical and Basolateral Membrane Expression of VEGFR2 and NRP2 in VEGF-A-stimulated Cultured Human Umbilical Vein Endothelial Cells.","authors":"Esmeralda K Bosma, Shahan Darwesh, Jia Y Zheng, Cornelis J F van Noorden, Reinier O Schlingemann, Ingeborg Klaassen","doi":"10.1369/00221554221115767","DOIUrl":"https://doi.org/10.1369/00221554221115767","url":null,"abstract":"Endothelial cells (ECs) form a precisely regulated polarized monolayer in capillary walls. Vascular endothelial growth factor-A (VEGF-A) induces endothelial hyperpermeability, and VEGF-A applied to the basolateral side, but not the apical side, has been shown to be a strong barrier disruptor in blood-retinal barrier ECs. We show here that VEGF-A presented to the basolateral side of human umbilical vein ECs (HUVECs) induces higher permeability than apical stimulation, which is similar to results obtained with bovine retinal ECs. We investigated with immunocytochemistry and confocal imaging the distribution of VEGF receptor-2 (VEGFR2) and neuropilin-2 (NRP2) in perinuclear apical and basolateral membrane domains. Orthogonal z-sections of cultured HUVECs were obtained, and the fluorescence intensity at the apical and basolateral membrane compartments was measured. We found that VEGFR2 and NRP2 are evenly distributed throughout perinuclear apical and basolateral membrane compartments in unstimulated HUVECs grown on Transwell inserts, whereas basolateral VEGF-A stimulation induces a shift toward basolateral VEGFR2 and NRP2 localization. When HUVECs were grown on coverslips, the distribution of VEGFR2 and NRP2 across the perinuclear apical and basolateral membrane domains was different. Our findings demonstrate that HUVECs dynamically regulate VEGFR2 and NRP2 localization on membrane microdomains, depending on growth conditions and the polarity of VEGF-A stimulation.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393510/pdf/10.1369_00221554221115767.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9905639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Expression of Calbindin-D28K in the Developing and Adult Mouse Cochlea. calbinding - d28k在发育和成年小鼠耳蜗中的表达。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2022-08-01 DOI: 10.1369/00221554221119543

Wenjing Liu, Huijun Chen, Xin Zhu, Hao Yu

{"title":"Expression of Calbindin-D28K in the Developing and Adult Mouse Cochlea.","authors":"Wenjing Liu, Huijun Chen, Xin Zhu, Hao Yu","doi":"10.1369/00221554221119543","DOIUrl":"https://doi.org/10.1369/00221554221119543","url":null,"abstract":"Herein, we aimed to use double-labeling immunofluorescence to describe the expression pattern of Calbindin-D28K (CaBP28K) in the mouse cochlea from late embryonic (E) stages to the adulthood. CaBP28K was expressed in the inner hair cells (IHCs) and the greater epithelial ridge (GER) at E17. In addition, its expression was observed in the interdental cells. On postnatal day 1 (P1), CaBP28K immunoreactivity was observed in the IHCs and outer hair cells (OHCs) and was also specifically expressed in the nucleus and the cytoplasm of spiral ganglion neurons (SGNs). At P8, CaBP28K labeling disappeared from the interdental cells, and the CaBP28K-positive domain within the GER shifted from the entire cytoplasm to only the apical and basal regions. At P14, CaBP28K immunoreactivity was lost from the GER; however, its expression in the IHCs and OHCs, as well as the SGNs, persisted into adulthood. The identification of CaBP28K in the hair cells (HCs) and cuticular plates, as well as SGNs, was confirmed by its colocalization with several markers for Sox2, Myosin VIIa, Phalloidin, and Tuj1. We also detected colocalization with calmodulin in the cytoplasm of both HCs and SGNs. Western blot revealed an increase in CaBP28K postnatal expression in the mouse cochlea.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393511/pdf/10.1369_00221554221119543.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9914987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Rapid Histological Assessment of Prostate Specimens in the Three-dimensional Space by Hydrophilic Tissue Clearing and Confocal Microscopy. 用亲水组织清除和共聚焦显微镜对三维空间前列腺标本的快速组织学评估。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2022-08-01 DOI: 10.1369/00221554221116936

Yu-Ching Peng, Yu-Chieh Lin, Yu-Ling Hung, Chien-Chung Fu, Margaret Dah-Tsyr Chang, Yen-Yin Lin, Teh-Ying Chou

{"title":"Rapid Histological Assessment of Prostate Specimens in the Three-dimensional Space by Hydrophilic Tissue Clearing and Confocal Microscopy.","authors":"Yu-Ching Peng, Yu-Chieh Lin, Yu-Ling Hung, Chien-Chung Fu, Margaret Dah-Tsyr Chang, Yen-Yin Lin, Teh-Ying Chou","doi":"10.1369/00221554221116936","DOIUrl":"https://doi.org/10.1369/00221554221116936","url":null,"abstract":"Microscopic examination of biopsied and resected prostatic specimens is the mainstay in the diagnosis of prostate cancer. However, conventional analysis of hematoxylin and eosin (H&E)-stained tissue is time-consuming and offers limited two-dimensional (2D) information. In the current study, we devised a method-termed Prostate Rapid Optical examination for cancer STATus (proSTAT)-for rapid screening of prostate cancer using high-resolution 2D and three-dimensional (3D) confocal images obtained after hydrophilic tissue clearing of 100-µm-thick tissue slices. The results of the proSTAT method were compared with those of traditional H&E stains for the analysis of cores (n=15) obtained from radical prostatectomy specimens (n=5). Gland lumen formation, consistent with Gleason pattern 3, was evident following tracking of multiple optical imaging sections. In addition, 3D rendering allowed visualizing a tubular network of interconnecting branches. Rapid 3D fluorescent labeling of tumor protein p63 accurately distinguished prostate adenocarcinoma from normal tissue and benign lesions. Compared with conventional stains, the 3D spatial and molecular information extracted from proSTAT may significantly increase the amount of available data for pathological assessment of prostate specimens. Our approach is amenable to automation and-subject to independent validation-can find a wide spectrum of clinical and research applications.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393508/pdf/10.1369_00221554221116936.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9914782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Relationship Between the Tumor Cell Expression of Hypoxic Markers and Survival in Patients With ER-positive Invasive Ductal Breast Cancer. er阳性浸润性导管乳腺癌患者肿瘤细胞缺氧标志物表达与生存的关系

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2022-07-01 Epub Date: 2022-07-06 DOI: 10.1369/00221554221110280

Suad A K Shamis, Jean Quinn, Elizabeth E A Mallon, Joanne Edwards, Donald C McMillan

{"title":"The Relationship Between the Tumor Cell Expression of Hypoxic Markers and Survival in Patients With ER-positive Invasive Ductal Breast Cancer.","authors":"Suad A K Shamis, Jean Quinn, Elizabeth E A Mallon, Joanne Edwards, Donald C McMillan","doi":"10.1369/00221554221110280","DOIUrl":"https://doi.org/10.1369/00221554221110280","url":null,"abstract":"The prognostic significance of hypoxia markers, hypoxia-inducible factor-1α (HIF-1α), hypoxia-inducible factor-2α (HIF-2α), and carbonic anhydrase IX (CAIX), was investigated in estrogen receptor (ER)-positive breast cancer patients. Immunohistochemistry determined the expression of makers in two independent ductal ER-positive cohorts (Training set, n=373 and Validation set, n=285) and was related to clinicopathological parameters and disease-free survival (DFS). In the training cohort, nuclear HIF-1α (1) was independently associated with poorer DFS in luminal A tumors [hazard ratio (HR) = 0.53 95% confidence interval (CI): 0.30-0.94, p=0.030]. In the validation cohort, both HIF-1α (1) and CAIX were independently associated with decreased DFS in the entire cohort (HR = 1.85 95% CI: 1.10-3.11, p=0.019; HR = 1.74 95% CI: 1.08-2.82, p=0.023), in luminal A disease (HR = 1.98 95% CI: 1.02-3.83, p=0.042), and in luminal B disease (HR = 2.75 95% CI: 1.66-4.55, p<0.001), respectively. Taken together, elevated cytoplasmic HIF-1α (1) expression was an independent prognostic factor in luminal A disease, whereas CAIX was an independent prognostic factor in luminal B disease. Further work in large tissue cohorts is required.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ab/a4/10.1369_00221554221110280.PMC9284237.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40563635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Morphology of Schwann Cell Processes Supports Renal Sympathetic Nerve Terminals With Local Distribution of Adrenoceptors. 雪旺细胞突形态支持肾交感神经末梢与肾上腺素能局部分布。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2022-07-01 DOI: 10.1369/00221554221106812

Seishi Maeda, Yusuke Minato, Sachi Kuwahara-Otani, Hiroki Yamanaka, Mitsuyo Maeda, Yosky Kataoka, Hideshi Yagi

{"title":"Morphology of Schwann Cell Processes Supports Renal Sympathetic Nerve Terminals With Local Distribution of Adrenoceptors.","authors":"Seishi Maeda, Yusuke Minato, Sachi Kuwahara-Otani, Hiroki Yamanaka, Mitsuyo Maeda, Yosky Kataoka, Hideshi Yagi","doi":"10.1369/00221554221106812","DOIUrl":"https://doi.org/10.1369/00221554221106812","url":null,"abstract":"Nerves in the renal parenchyma comprise sympathetic nerves that act on renal arteries and tubules to decrease blood flow and increase primary urine reabsorption, respectively. Synaptic vesicles release neurotransmitters that activate their effector tissues. However, the mechanisms by which neurotransmitters exert individual responses to renal effector cells remain unknown. Here, we investigated the spatial and molecular compositional associations of renal Schwann cells (SC) supporting the nerve terminals in male rats. The nerve terminals of vascular smooth muscle cells (SMCs) enclosed by renal SC processes were exposed through windows facing the effectors with presynaptic specializations. We found that the adrenergic receptors (ARs) α2A, α2C, and β2 were localized in the SMC and the basal side of the tubules, where the nerve terminals were attached, whereas the other subtypes of ARs were distributed in the glomerular and luminal side, where the norepinephrine released from nerve endings may have indirect access to ARs. In addition, integrins α4 and β1 were coexpressed in the nerve terminals. Thus, renal nerve terminals could contact their effectors via integrins and may have a structure, covered by SC processes, suitable for intensive and directional release of neurotransmitters into the blood, rather than specialized structures in the postsynaptic region.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284235/pdf/10.1369_00221554221106812.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9786760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased Colocalization and Interaction Between Decidual Protein Kinase A and Insulin-like Growth Factor-Binding Protein-1 in Intrauterine Growth Restriction. 蜕膜蛋白激酶A和胰岛素样生长因子结合蛋白-1在宫内生长受限中的共定位和相互作用增加。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2022-07-01 DOI: 10.1369/00221554221112702

Madhulika B Gupta, Kyle K Biggar, Cun Li, Peter W Nathanielsz, Thomas Jansson

{"title":"Increased Colocalization and Interaction Between Decidual Protein Kinase A and Insulin-like Growth Factor-Binding Protein-1 in Intrauterine Growth Restriction.","authors":"Madhulika B Gupta, Kyle K Biggar, Cun Li, Peter W Nathanielsz, Thomas Jansson","doi":"10.1369/00221554221112702","DOIUrl":"https://doi.org/10.1369/00221554221112702","url":null,"abstract":"Increased phosphorylation of decidual insulin-like growth factor-binding protein-1 (IGFBP-1) can contribute to intrauterine growth restriction (IUGR) by decreasing the bioavailability of insulin-like growth factor-1 (IGF-1). However, the molecular mechanisms regulating IGFBP-1 phosphorylation at the maternal-fetal interface are poorly understood. Protein kinase A (PKA) is required for normal decidualization. Consensus sequences for PKA are present in IGFBP-1. We hypothesized that the expression/interaction of PKA with decidual IGFBP-1 is increased in IUGR. Parallel reaction monitoring-mass spectrometry (PRM-MS) identified multiple PKA peptides (n=>30) co-immunoprecipitating with IGFBP-1 in decidualized primary human endometrial stromal cells (HESC). PRM-MS also detected active PKApThr197 and greater site-specific IGFBP-1 phosphorylation(pSer119), (pSer98+pSer101) (pSer169+pSer174) in response to hypoxia. Hypoxia promoted colocalization [dual immunofluorescence (IF)] of PKA with IGFBP-1 in decidualized HESC. Colocalization (IF) and interaction (proximity ligation assay) of PKA and IGFBP-1 were increased in decidua collected from placenta of human IUGR pregnancies (n=8) compared with decidua from pregnancies with normal fetal growth. Similar changes were detected in decidual PKA/IGFBP-1 using placenta from baboons subjected to maternal nutrient reduction (MNR) vs controls (n=3 each). In baboons, these effects were evident in MNR at gestational day 120 prior to IUGR onset. Increased PKA-mediated phosphorylation of decidual IGFBP-1 may contribute to decreased IGF-1 bioavailability in the maternal-fetal interface in IUGR.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9284236/pdf/10.1369_00221554221112702.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10091267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0