Colonic Myenteric Plexus Neurodegeneration and Minor Colon Inflammation in Trimethyltin-induced Rat Model of Neurodegeneration.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-06-01 Epub Date: 2023-06-15 DOI:10.1369/00221554231182195

Dian Eurike Septyaningtrias, Hilizza Awalina Zulfa, Mahayu Firsty Ramadhani, Sumaryati, Dewi Sulistyawati, Dewi Kartikawati Paramita, Yustina Andwi Ari Sumiwi, Rina Susilowati

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引用次数: 0

Abstract

Gastrointestinal symptoms are common health problems found during aging and neurodegenerative diseases. Trimethyltin-induced rat is known as an animal model of hippocampal degeneration with no data on enteric neurodegeneration. This study aimed to investigate the effect of trimethyltin (TMT) induction on the gastrointestinal tract. A 28-day animal study with male Sprague-Dawley rats (3 months old, 150-200 g) given a single TMT injection (8 mg/kg body weight, intraperitoneal) was conducted. The number of neurons in the colonic myenteric plexus was measured using stereological estimation. Histological scoring of colon inflammation, immunohistochemistry of tumor necrosis factor-α (TNF-α), and quantitative PCR were conducted. This study showed neuronal loss in the colonic myenteric plexus of TMT-induced rat model of neurodegeneration. Minor colon inflammation characterized by inflammatory cell infiltration and slightly higher expression of TNF-α in the colon mucosa were observed in the TMT-induced rat. However, the gut microbiota composition of the TMT-induced rat was not different from that of the control rats. This study demonstrates that TMT induces colonic myenteric plexus neurodegeneration and minor colon inflammation, which suggests the potential of this animal model to elucidate the communication between the gastrointestinal tract and central nervous system in neurodegenerative diseases.

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三甲基锡诱导的大鼠神经变性模型中的结肠肠系膜神经丛神经变性和轻微结肠炎症

胃肠道症状是衰老和神经退行性疾病中常见的健康问题。三甲基锡诱导大鼠是众所周知的海马变性动物模型，但没有关于肠道神经变性的数据。本研究旨在调查三甲基锡（TMT）诱导对胃肠道的影响。研究人员对雄性 Sprague-Dawley 大鼠（3 个月大，体重 150-200 克）进行了为期 28 天的动物实验，给予单次 TMT 注射（8 毫克/千克体重，腹腔注射）。采用立体估算法测量结肠肠肌丛神经元的数量。对结肠炎症进行了组织学评分、肿瘤坏死因子-α（TNF-α）免疫组化和定量 PCR。研究结果表明，TMT诱导的神经变性大鼠结肠肠肌丛神经元丢失。在 TMT 诱导的大鼠中观察到了轻微的结肠炎症，其特征是炎性细胞浸润和 TNF-α 在结肠粘膜中的表达略高。然而，TMT 诱导的大鼠的肠道微生物群组成与对照组大鼠并无不同。这项研究表明，TMT可诱导结肠肠肌丛神经变性和轻微的结肠炎症，这表明该动物模型具有阐明神经退行性疾病中胃肠道和中枢神经系统之间沟通的潜力。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.