Journal of Histochemistry & Cytochemistry最新文献_第9页

Commentary on a Classic JHC Article on the Development of Highly Sensitive Fluorochrome-labeled Tyramides for Immunocytochemistry. 评论 JHC 有关开发用于免疫细胞化学的高灵敏度荧光标记酪胺的经典文章。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2023-05-01 Epub Date: 2023-05-18 DOI: 10.1369/00221554231178068

Kevin A Roth

引用次数: 0

Epitope Lability of Phosphorylated Biomarkers of the DNA Damage Response Pathway Results in Increased Vulnerability to Effects of Delayed or Incomplete Formalin Fixation. DNA 损伤应答途径磷酸化生物标记物的表位易变性导致更易受延迟或不完全福尔马林固定的影响。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2023-05-01 Epub Date: 2023-04-29 DOI: 10.1369/00221554231174069

Elizabeth J Wiseman, Jennifer I Moss, James Atkinson, Hana Baakza, Emily Hayes, Sophie E Willis, Paul M Waring, Jaime Rodriguez Canales, Gemma N Jones

{"title":"Epitope Lability of Phosphorylated Biomarkers of the DNA Damage Response Pathway Results in Increased Vulnerability to Effects of Delayed or Incomplete Formalin Fixation.","authors":"Elizabeth J Wiseman, Jennifer I Moss, James Atkinson, Hana Baakza, Emily Hayes, Sophie E Willis, Paul M Waring, Jaime Rodriguez Canales, Gemma N Jones","doi":"10.1369/00221554231174069","DOIUrl":"10.1369/00221554231174069","url":null,"abstract":"Phosphorylated biomarkers are crucial for our understanding of drug mechanism of action and dose selection during clinical trials, particularly for drugs that target protein kinases, such as DNA-damage-response (DDR) inhibitors. However, tissue fixation conditions needed to preserve DDR-specific phospho-biomarkers have not been previously investigated. Using xenograft tissues and tightly controlled formalin fixation conditions, we assessed how preanalytical factors affect phosphorylated DDR biomarkers pRAD50(Ser635), ɣH2AX(Ser139), pKAP1(Ser824), and non-phosphorylated biomarkers cMYC and ATM. Cold ischemia times ranged from 15 min to 6 hr, and the fixation duration ranged from 24 hr to 4 weeks. Epitopes pRAD50 and pKAP1 appeared the most labile assessed with staining loss after just 15 min of cold ischemia time, while ATM was more robust showing consistent expression up to 1 hr of cold ischemia. Notably, ɣH2AX expression was lost with formalin fixation over 48 hr. The use of core needle biopsies where possible and novel fixation methods such as the 2-step temperature-controlled formalin approach may improve phosphorylated biomarker preservation; however, practical challenges may affect wider clinical application. The most essential tissue-processing step when downstream analysis includes DDR phosphorylated biomarkers is immediate tissue submersion in formalin, without delay, upon excision from the patient, followed by room temperature fixation for 24 hr.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":"71 5","pages":"237-257"},"PeriodicalIF":3.2,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10227880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9659316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Commentary on Classic Article. 经典文章评论。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2023-04-01 Epub Date: 2023-04-04 DOI: 10.1369/00221554231166740

Constance Oliver

引用次数: 0

The Distribution of Foxp3 and CD68 in Preeclamptic and Healthy Placentas: A Histomorphological Evaluation. Foxp3 和 CD68 在先兆子痫和健康胎盘中的分布：组织形态学评估

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2023-04-01 Epub Date: 2023-04-18 DOI: 10.1369/00221554231170662

Yasemin Ersoy Canillioglu, Gozde Erkanli Senturk, Hakan Sahin, Sadik Sahin, Yasemin Seval-Celik

{"title":"The Distribution of Foxp3 and CD68 in Preeclamptic and Healthy Placentas: A Histomorphological Evaluation.","authors":"Yasemin Ersoy Canillioglu, Gozde Erkanli Senturk, Hakan Sahin, Sadik Sahin, Yasemin Seval-Celik","doi":"10.1369/00221554231170662","DOIUrl":"10.1369/00221554231170662","url":null,"abstract":"Preeclampsia is a complication of pregnancy that affects 3-5% of pregnancies and is one of the major causes of maternal/neonatal mortality and morbidities worldwide. We aimed to investigate the distribution of Foxp3+ regulatory T-cells and CD68+ Hofbauer cells in the placenta of preeclamptic and healthy pregnant women with a special focus on correlating these findings with placental histology. Decidua and chorionic villi of the placenta obtained from healthy and preeclamptic pregnancies were evaluated in full-thickness sections. Sections were stained with hematoxylin and eosin and Masson's trichrome and immunostained for Foxp3 and CD68 for histological analyses. The total histomorphological score for placentas was found to be higher in preeclamptic placentas than that in the controls. The CD68 immunoreactivity was higher in the chorionic villi of preeclamptic placentas than that in the controls. The immunoreactivity of Foxp3 was found widely distributed within the decidua in both the groups and did not differ significantly. Interestingly, Foxp3 immunoreactivity in the chorionic villi was found mainly in the villous core and, to a lesser extent, in the syncytiotrophoblasts. We found no significant relation between Foxp3 expressions and morphological changes observed in preeclamptic placentas. Although extensive research is being carried out regarding the pathophysiology of preeclampsia, the findings are still controversial.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":"71 4","pages":"211-225"},"PeriodicalIF":3.2,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10149892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9651112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial Distribution of SARS-CoV-2 Receptors and Proteases in Testicular Cells. SARS-CoV-2受体和蛋白酶在睾丸细胞中的空间分布

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2023-04-01 DOI: 10.1369/00221554231168916

Maria Rita Ribeiro, Ana Margarida Calado, Ângela Alves, Rute Pereira, Mário Sousa, Rosália Sá

{"title":"Spatial Distribution of SARS-CoV-2 Receptors and Proteases in Testicular Cells.","authors":"Maria Rita Ribeiro, Ana Margarida Calado, Ângela Alves, Rute Pereira, Mário Sousa, Rosália Sá","doi":"10.1369/00221554231168916","DOIUrl":"https://doi.org/10.1369/00221554231168916","url":null,"abstract":"Coronavirus disease (COVID-19) is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). SARS-CoV-2 RNA has been found in the human testis on occasion, but subgenomic SARS-CoV-2 and infectious SARS-CoV-2 virions have not been found. There is no direct evidence of SARS-CoV-2 infection of testicular cells. To better understand this, it is necessary to determine whether SARS-CoV-2 receptors and proteases are present in testicular cells. To overcome this limitation, we focused on elucidating with immunohistochemistry the spatial distribution of the SARS-CoV-2 receptors angiotensin-converting enzyme 2 (ACE2) and cluster of differentiation 147 (CD147), as well as their viral spike protein priming proteases, transmembrane protease serine 2 (TMPRSS2) and cathepsin L (CTSL), required for viral fusion with host cells. At the protein level, human testicular tissue expressed both receptors and proteases studied. Both ACE2 and TMPRSS2 were found in interstitial cells (endothelium, Leydig, and myoid peritubular cells) and in the seminiferous epithelium (Sertoli cells, spermatogonia, spermatocytes, and spermatids). The presence of CD147 was observed in all cell types except endothelium and peritubular cells, while CTSL was exclusively observed in Leydig, peritubular, and Sertoli cells. These findings show that the ACE2 receptor and its protease TMPRSS2 are coexpressed in all testicular cells, as well as the CD147 receptor and its protease CTSL in Leydig and Sertoli cells, indicating that testicular SARS-CoV-2 infection cannot be ruled out without further investigation.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":"71 4","pages":"169-197"},"PeriodicalIF":3.2,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a6/77/10.1369_00221554231168916.PMC10083717.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9650699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Betaine Treatment Prevents TNF-α-Mediated Muscle Atrophy by Restoring Total Protein Synthesis Rate and Morphology in Cultured Myotubes. 甜菜碱治疗可通过恢复培养肌管的总蛋白合成率和形态来防止 TNF-α 介导的肌肉萎缩

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2023-04-01 Epub Date: 2023-04-03 DOI: 10.1369/00221554231165326

Andrea Di Credico, Giulia Gaggi, Pascal Izzicupo, Daniela Vitucci, Pasqualina Buono, Angela Di Baldassarre, Barbara Ghinassi

{"title":"Betaine Treatment Prevents TNF-α-Mediated Muscle Atrophy by Restoring Total Protein Synthesis Rate and Morphology in Cultured Myotubes.","authors":"Andrea Di Credico, Giulia Gaggi, Pascal Izzicupo, Daniela Vitucci, Pasqualina Buono, Angela Di Baldassarre, Barbara Ghinassi","doi":"10.1369/00221554231165326","DOIUrl":"10.1369/00221554231165326","url":null,"abstract":"Skeletal muscle atrophy is represented by a dramatic decrease in muscle mass, and it is related to a lower life expectancy. Among the different causes, chronic inflammation and cancer promote protein loss through the effect of inflammatory cytokines, leading to muscle shrinkage. Thus, the availability of safe methods to counteract inflammation-derived atrophy is of high interest. Betaine is a methyl derivate of glycine and it is an important methyl group donor in transmethylation. Recently, some studies found that betaine could promote muscle growth, and it is also involved in anti-inflammatory mechanisms. Our hypothesis was that betaine would be able to prevent tumor necrosis factor-α (TNF-α)-mediated muscle atrophy in vitro. We treated differentiated C2C12 myotubes for 72 hr with either TNF-α, betaine, or a combination of them. After the treatment, we analyzed total protein synthesis, gene expression, and myotube morphology. Betaine treatment blunted the decrease in muscle protein synthesis rate exerted by TNF-α, and upregulated Mhy1 gene expression in both control and myotube treated with TNF-α. In addition, morphological analysis revealed that myotubes treated with both betaine and TNF-α did not show morphological features of TNF-α-mediated atrophy. We demonstrated that in vitro betaine supplementation counteracts the muscle atrophy led by inflammatory cytokines.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":"71 4","pages":"199-209"},"PeriodicalIF":3.2,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10149894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10015293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of β-Catenin, E-Cadherin, and α-Smooth Muscle Actin in Basal Cell Carcinoma Before Photodynamic Therapy in Non-recurrent and Recurrent Tumors: Exploring the Ability of Predicting Photodynamic Therapy Outcome. 非复发性和复发性肿瘤光动力疗法前基底细胞癌中β-Catenin、E-Cadherin和α-平滑肌肌动蛋白的表达：探索光动力疗法结果的预测能力

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2023-03-01 Epub Date: 2023-03-24 DOI: 10.1369/00221554231161396

Erik Mørk, Patricia Mjønes, Olav A Foss, Ingeborg M Bachmann, Eidi Christensen

{"title":"Expression of β-Catenin, E-Cadherin, and α-Smooth Muscle Actin in Basal Cell Carcinoma Before Photodynamic Therapy in Non-recurrent and Recurrent Tumors: Exploring the Ability of Predicting Photodynamic Therapy Outcome.","authors":"Erik Mørk, Patricia Mjønes, Olav A Foss, Ingeborg M Bachmann, Eidi Christensen","doi":"10.1369/00221554231161396","DOIUrl":"10.1369/00221554231161396","url":null,"abstract":"Photodynamic therapy (PDT) is an effective and cosmetically beneficial treatment of low-risk basal cell carcinomas (BCCs). To optimize PDT response, it is important to correctly select tumors. We sought to find markers that could identify such tumors beyond contributions from clinical and histological examination. Studies have shown that β-catenin, E-cadherin, and α-smooth muscle actin (SMA) expression can indicate BCC aggressiveness/BCC invasiveness. We wanted to use these markers in an explorative study to investigate whether they were differently expressed among non-recurring compared with recurring BCCs, to evaluate their ability of predicting PDT outcome. Fifty-two BCCs were stained with antibodies against β-catenin, E-cadherin, and α-SMA, and evaluated using immunoreactive score (IRS), subcellular localization, and stromal protein expression. Results showed that IRS of E-cadherin was significantly different among recurring compared with non-recurring BCCs and with area under a receiver operating characteristic curve of 0.71 (95% confidence interval: 0.56-0.86, p=0.025). Stromal β-catenin expression significantly increased among recurring BCCs. Some recurring BCCs had intense expression in the deep invading tumor edge. In conclusion, E-cadherin, and stromal and deep edge β-catenin expression were most prominent in BCCs that recurred post-PDT, suggesting they could potentially predict PDT outcome. Further studies are needed to investigate whether these results are of clinical value.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":"71 3","pages":"111-120"},"PeriodicalIF":3.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9663169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplex Immunofluorescence Image Quality Checking Using DAPI Channel-referenced Evaluation. 利用 DAPI 通道参照评估进行多重免疫荧光图像质量检查

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2023-03-01 Epub Date: 2023-03-24 DOI: 10.1369/00221554231161693

Jun Jiang, Raymond Moore, Clarissa E Jordan, Ruifeng Guo, Rachel L Maus, Hongfang Liu, Ellen Goode, Svetomir N Markovic, Chen Wang

{"title":"Multiplex Immunofluorescence Image Quality Checking Using DAPI Channel-referenced Evaluation.","authors":"Jun Jiang, Raymond Moore, Clarissa E Jordan, Ruifeng Guo, Rachel L Maus, Hongfang Liu, Ellen Goode, Svetomir N Markovic, Chen Wang","doi":"10.1369/00221554231161693","DOIUrl":"10.1369/00221554231161693","url":null,"abstract":"Multiplex immunofluorescence (MxIF) images provide detailed information of cell composition and spatial context for biomedical research. However, compromised data quality could lead to research biases. Comprehensive image quality checking (QC) is essential for reliable downstream analysis. As a reliable and specific staining of cell nuclei, 4',6-diamidino-2-phenylindole (DAPI) signals were used as references for tissue localization and auto-focusing across MxIF staining-scanning-bleaching iterations and could potentially be reused for QC. To confirm the feasibility of using DAPI as QC reference, pixel-level DAPI values were extracted to calculate signal fluctuations and tissue content similarities in staining-scanning-bleaching iterations for identifying quality issues. Concordance between automatic quantification and human experts' annotations were evaluated on a data set consisting of 348 fields of view (FOVs) with 45 immune and tumor cell markers. Cell distribution differences between subsets of QC-pass vs QC-failed FOVs were compared to investigate the downstream effects. Results showed that 87.3% FOVs with tissue damage and 73.4% of artifacts were identified. QC-failed FOVs showed elevated regional gathering in cellular feature space compared with the QC-pass FOVs. Our results supported that DAPI signals could be used as references for MxIF image QC, and low-quality FOVs identified by our method must be cautiously considered for downstream analyses.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":"71 3","pages":"121-130"},"PeriodicalIF":3.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9660061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of RANKL Expression in Osteocyte-like Differentiated Tumor Cells in Giant Cell Tumor of Bone After Denosumab Treatment. 地诺单抗治疗后抑制骨巨细胞瘤中骨细胞样分化肿瘤细胞的 RANKL 表达

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2023-03-01 Epub Date: 2023-03-27 DOI: 10.1369/00221554231163638

Takashi Noguchi, Akio Sakamoto, Yoshiki Murotani, Koichi Murata, Masahiro Hirata, Yosuke Yamada, Junya Toguchida, Shuichi Matsuda

{"title":"Inhibition of RANKL Expression in Osteocyte-like Differentiated Tumor Cells in Giant Cell Tumor of Bone After Denosumab Treatment.","authors":"Takashi Noguchi, Akio Sakamoto, Yoshiki Murotani, Koichi Murata, Masahiro Hirata, Yosuke Yamada, Junya Toguchida, Shuichi Matsuda","doi":"10.1369/00221554231163638","DOIUrl":"10.1369/00221554231163638","url":null,"abstract":"Giant cell tumors of bone (GCTBs) are locally aggressive tumors with the histological features of giant cells and stromal cells. Denosumab is a human monoclonal antibody that binds to the cytokine receptor activator of nuclear factor-kappa B ligand (RANKL). RANKL inhibition blocks tumor-induced osteoclastogenesis, and survival, and is used to treat unresectable GCTBs. Denosumab treatment induces osteogenic differentiation of GCTB cells. In this study, the expression of RANKL, special AT-rich sequence-binding protein 2 (SATB2, a marker of osteoblast differentiation), and sclerostin/SOST (a marker of mature osteocytes) was analyzed before and after treatment with denosumab in six cases of GCTB. Denosumab therapy was administered a mean of five times over a mean 93.5-day period. Before denosumab treatment, RANKL expression was observed in one of six cases. After denosumab therapy, spindle-like cells devoid of giant cell aggregation were RANKL-positive in four of six cases. Bone matrix-embedded osteocyte markers were observed, although RANKL was not expressed. Osteocyte-like cells were confirmed to have mutations, as identified using mutation-specific antibodies. Our study results suggest that treatment of GCTBs with denosumab results in osteoblast-osteocyte differentiation. Denosumab played a role in the suppression of tumor activity via inhibition of the RANK-RANKL pathway, which triggers osteoclast precursors to differentiate into osteoclasts.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":"71 3","pages":"131-138"},"PeriodicalIF":3.2,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10032743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thioflavin S Staining and Amyloid Formation Are Unique to Mixed Tauopathies. 硫黄素 S 染色和淀粉样蛋白形成是混合型 Tauopathies 的独特之处。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2023-02-01 Epub Date: 2023-03-02 DOI: 10.1369/00221554231158428

Kimberly L Fiock, Ryan K Betters, Marco M Hefti

{"title":"Thioflavin S Staining and Amyloid Formation Are Unique to Mixed Tauopathies.","authors":"Kimberly L Fiock, Ryan K Betters, Marco M Hefti","doi":"10.1369/00221554231158428","DOIUrl":"10.1369/00221554231158428","url":null,"abstract":"Tau phosphorylation, aggregation, and toxicity are the main drivers of neurodegeneration in multiple tauopathies, including Alzheimer's disease (AD) and frontotemporal lobar degeneration with tau. Although aggregation and amyloid formation are often assumed to be synonymous, the ability of tau aggregates in different diseases to form amyloids in vivo has not been systematically studied. We used the amyloid dye Thioflavin S to look at tau aggregates in mixed tauopathies such as AD and primary age-related tauopathy, as well as pure 3R or 4R tauopathies such as Pick's disease, progressive supranuclear palsy, and corticobasal degeneration. We found that aggregates of tau protein only form thioflavin-positive amyloids in mixed (3R/4R), but not pure (3R or 4R), tauopathies. Interestingly, neither astrocytic nor neuronal tau pathology was thioflavin-positive in pure tauopathies. As most current positron emission tomography tracers are based on thioflavin derivatives, this suggests that they may be more useful for differential diagnosis than the identification of a general tauopathy. Our findings also suggest that thioflavin staining may have utility as an alternative to traditional antibody staining for distinguishing between tau aggregates in patients with multiple pathologies and that the mechanisms for tau toxicity may differ between different tauopathies.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":"71 2","pages":"73-86"},"PeriodicalIF":3.2,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10071402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9287448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0