Journal of Histochemistry & Cytochemistry最新文献_第3页

HMGB2 Promotes De Novo Lipogenesis to Accelerate Hepatocyte Proliferation During Liver Regeneration. HMGB2 在肝脏再生过程中促进新脂肪生成以加速肝细胞增殖

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-04-01 Epub Date: 2024-03-27 DOI: 10.1369/00221554241241569

Narantsog Choijookhuu, Koichi Yano, Baljinnyam Lkham-Erdene, Shinichiro Shirouzu, Toshiki Kubota, Fidya, Takumi Ishizuka, Kengo Kai, Etsuo Chosa, Yoshitaka Hishikawa

{"title":"HMGB2 Promotes De Novo Lipogenesis to Accelerate Hepatocyte Proliferation During Liver Regeneration.","authors":"Narantsog Choijookhuu, Koichi Yano, Baljinnyam Lkham-Erdene, Shinichiro Shirouzu, Toshiki Kubota, Fidya, Takumi Ishizuka, Kengo Kai, Etsuo Chosa, Yoshitaka Hishikawa","doi":"10.1369/00221554241241569","DOIUrl":"10.1369/00221554241241569","url":null,"abstract":"Liver regeneration is a well-orchestrated compensatory process that is regulated by multiple factors. We recently reported the importance of the chromatin protein, a high-mobility group box 2 (HMGB2) in mouse liver regeneration. However, the molecular mechanism remains unclear. In this study, we aimed to study how HMGB2 regulates hepatocyte proliferation during liver regeneration. Seventy-percent partial hepatectomy (PHx) was performed in wild-type (WT) and HMGB2-knockout (KO) mice, and the liver tissues were used for microarray, immunohistochemistry, quantitative polymerase chain reaction (qPCR), and Western blotting analyses. In the WT mice, HMGB2-positive hepatocytes colocalized with cell proliferation markers. In the HMGB2-KO mice, hepatocyte proliferation was significantly decreased. Oil Red O staining revealed the transient accumulation of lipid droplets at 12-24 hr after PHx in the WT mouse livers. In contrast, decreased amount of lipid droplets were found in HMGB2-KO mouse livers, and it was preserved until 36 hr. The microarray, immunohistochemistry, and qPCR results demonstrated that the expression of lipid metabolism-related genes was significantly decreased in the HMGB2-KO mouse livers. The in vitro experiments demonstrated that a decrease in the amount of lipid droplets correlated with decreased cell proliferation activity in HMGB2-knockdown cells. HMGB2 promotes de novo lipogenesis to accelerate hepatocyte proliferation during liver regeneration.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11020747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transient Receptor Potential Channels in the Healthy and Diseased Blood-Brain Barrier. 健康和患病血脑屏障中的瞬态受体电位通道

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-04-01 Epub Date: 2024-04-08 DOI: 10.1369/00221554241246032

Rita Rezzani, Gaia Favero, Marzia Gianò, Daniela Pinto, Mauro Labanca, Cornelis J F van Noorden, Fabio Rinaldi

{"title":"Transient Receptor Potential Channels in the Healthy and Diseased Blood-Brain Barrier.","authors":"Rita Rezzani, Gaia Favero, Marzia Gianò, Daniela Pinto, Mauro Labanca, Cornelis J F van Noorden, Fabio Rinaldi","doi":"10.1369/00221554241246032","DOIUrl":"10.1369/00221554241246032","url":null,"abstract":"The large family of transient receptor potential (TRP) channels are integral membrane proteins that function as environmental sensors and act as ion channels after activation by mechanical (touch), physical (heat, pain), and chemical stimuli (pungent compounds such as capsaicin). Most TRP channels are localized in the plasma membrane of cells but some of them are localized in membranes of organelles and function as intracellular Ca2+-ion channels. TRP channels are involved in neurological disorders but their precise role(s) and relevance in these disorders are not clear. Endothelial cells of the blood-brain barrier (BBB) express TRP channels such as TRP vanilloid 1-4 and are involved in thermal detection by regulating BBB permeability. In neurological disorders, TRP channels in the BBB are responsible for edema formation in the brain. Therefore, drug design to modulate locally activity of TRP channels in the BBB is a hot topic. Today, the application of TRP channel antagonists against neurological disorders is still limited.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11020746/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140860300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clearing Properties Between Coconut Oil and Xylene in Histological Tissue Processing. 椰子油和二甲苯在组织学组织处理中的清除特性

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-04-01 Epub Date: 2024-03-30 DOI: 10.1369/00221554241241369

Owusu Afriyie Bright, Darko Nkansah Samuel, Musah Ayeley Adisa, Owusu Ohui Dorcas, Quartey Perez, Antwi Ama Melody, Addai Kusi Michael, Osei Sarpong Albert, Ebenezer Senu

{"title":"Clearing Properties Between Coconut Oil and Xylene in Histological Tissue Processing.","authors":"Owusu Afriyie Bright, Darko Nkansah Samuel, Musah Ayeley Adisa, Owusu Ohui Dorcas, Quartey Perez, Antwi Ama Melody, Addai Kusi Michael, Osei Sarpong Albert, Ebenezer Senu","doi":"10.1369/00221554241241369","DOIUrl":"10.1369/00221554241241369","url":null,"abstract":"Xylene is the commonest clearing agent even though it is hazardous and costly. This study evaluated the clearing properties of coconut oil as an alternative cost-effective clearing agent for histological processes. Ten (10) prostate samples fixed in formalin were taken and each one was cut into 4 before randomly separating them into four groups (A, B, C and D). Tissues were subjected to ascending grades of alcohol for dehydration. Group A was cleared in xylene and Groups B, C, and D were cleared at varying times of 1hr 30mins, 3hrs, and 4hrs in coconut oil respectively before embedding, sectioning, and staining were carried out. Gross and histological features were compared. Results indicated a significant shrinkage in coconut oil-treated specimen compared with the xylene-treated specimen and only the tissues cleared in coconut oil for 4hrs were as rigid as the tissues cleared in xylene (p > 0.05). No significant difference was found in either of the sections when checked for cellular details and staining quality (p > 0.999). Coconut oil is an efficient substitute for xylene in prostate tissues with a minimum clearing time of 4hrs, as it is environmentally friendly and less expensive, but causes significant shrinkage to prostate tissue.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11020745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140326681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diabetes as a Pancreatic Microvascular Disease-A Pericytic Perspective. 糖尿病是一种胰腺微血管疾病--胰周病的视角。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-03-01 Epub Date: 2024-03-07 DOI: 10.1369/00221554241236535

Luciana Mateus Gonçalves, Catarina Andrade Barboza, Joana Almaça

{"title":"Diabetes as a Pancreatic Microvascular Disease-A Pericytic Perspective.","authors":"Luciana Mateus Gonçalves, Catarina Andrade Barboza, Joana Almaça","doi":"10.1369/00221554241236535","DOIUrl":"10.1369/00221554241236535","url":null,"abstract":"Diabetes is not only an endocrine but also a vascular disease. Vascular defects are usually seen as consequence of diabetes. However, at the level of the pancreatic islet, vascular alterations have been described before symptom onset. Importantly, the cellular and molecular mechanisms underlying these early vascular defects have not been identified, neither how these could impact the function of islet endocrine cells. In this review, we will discuss the possibility that dysfunction of the mural cells of the microvasculature-known as pericytes-underlies vascular defects observed in islets in pre-symptomatic stages. Pericytes are crucial for vascular homeostasis throughout the body, but their physiological and pathophysiological functions in islets have only recently started to be explored. A previous study had already raised interest in the \"microvascular\" approach to this disease. With our increased understanding of the crucial role of the islet microvasculature for glucose homeostasis, here we will revisit the vascular aspects of islet function and how their deregulation could contribute to diabetes pathogenesis, focusing in particular on type 1 diabetes (T1D).","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10956440/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140059588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special Topics Series: Special Issue on Endothelial Cell and Pericyte Homeostasis and Pathogenesis. 专题系列：内皮细胞和周皮稳态与发病机制特刊》。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-03-01 DOI: 10.1369/00221554241240841

Stephen M Hewitt

引用次数: 0

Imaging of Isolated Exosomes by Correlative Microscopy. 利用相关显微镜对分离的外泌体进行成像。

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-03-01 Epub Date: 2024-02-24 DOI: 10.1369/00221554241233346

Şeyda Demir, Esra Erdal, Hüsnü Alper Bagriyanik

{"title":"Imaging of Isolated Exosomes by Correlative Microscopy.","authors":"Şeyda Demir, Esra Erdal, Hüsnü Alper Bagriyanik","doi":"10.1369/00221554241233346","DOIUrl":"10.1369/00221554241233346","url":null,"abstract":"Correlative microscopy is a sophisticated imaging technique that combines optical and electron microscopes, with the most common approach being the integration of light microscopy and electron microscopy, known as correlative light and electron microscopy (CLEM). While CLEM provides a comprehensive view of biological samples, it presents a significant challenge in sample preparation due to the distinct processes involved in each technique. Striking a balance between these methods is crucial. Despite numerous approaches, achieving seamless imaging with CLEM remains a complex task. Exosomes, nanovesicles ranging from 30 to 150 nm in size, are enclosed by a lipid bilayer and released by various cell types. Visualizing exosomes poses difficulties due to their small size and minimal electric charge. However, imaging exosomes at high resolution offers a direct method to understand their morphology and functions. In this study, we evaluated exosome imaging with CLEM using a combination of confocal, transmission electron microscope, and scanning electron microscope (SEM). In addition, we conducted a comparative analysis of these two techniques, evaluating their suitability and efficiency in imaging nanoscale structures. In this study, we found that confocal-SEM correlation is more applicable for imaging exosomes. Moreover, we observed that exosomes were found in clusters in confocal-SEM correlation.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10956441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139944178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relation Between Reactive Oxygen Species Production and Transient Receptor Potential Vanilloid1 Expression in Human Skin During Aging. 人体皮肤老化过程中活性氧生成与瞬时受体电位 Vanilloid1 表达之间的关系

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-03-01 Epub Date: 2024-03-05 DOI: 10.1369/00221554241236537

Gaia Favero, Marzia Gianò, Caterina Franco, Daniela Pinto, Cornelis J F van Noorden, Fabio Rinaldi, Rita Rezzani

引用次数: 0

Relevance of Comet Assay and Phosphorylated-Hsp90α in Cancer Patients' Peripheral Blood Leukocytes as Tools to Assess Cisplatin-based Chemotherapy Clinical Response and Disease Outcome. 癌症患者外周血白细胞中的彗星试验和磷酸化-Hsp90α作为评估顺铂化疗临床反应和疾病预后的工具的相关性

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-03-01 Epub Date: 2024-03-05 DOI: 10.1369/00221554241236241

Mayra L Sottile, Laura C Gómez, Analía Redondo, Jorge Ibarra, María B García, Lucía Gonzalez, Laura M Vargas-Roig, Silvina B Nadin

{"title":"Relevance of Comet Assay and Phosphorylated-Hsp90α in Cancer Patients' Peripheral Blood Leukocytes as Tools to Assess Cisplatin-based Chemotherapy Clinical Response and Disease Outcome.","authors":"Mayra L Sottile, Laura C Gómez, Analía Redondo, Jorge Ibarra, María B García, Lucía Gonzalez, Laura M Vargas-Roig, Silvina B Nadin","doi":"10.1369/00221554241236241","DOIUrl":"10.1369/00221554241236241","url":null,"abstract":"Cisplatin (cPt) is a commonly used treatment for solid tumors. The main target of its cytotoxicity is the DNA molecule, which makes the DNA damage response (DDR) crucial for cPt-based chemotherapy. Therefore, it is essential to identify biomarkers that can accurately predict the individual clinical response and prognosis. Our goal was to assess the usefulness of alkaline comet assay and immunocytochemical staining of phosphorylated Hsp90α (p-Hsp90α), γH2AX, and 53BP1 as predictive/prognostic markers. Pre-chemotherapy peripheral blood leukocytes were exposed to cPt in vitro and collected at 0, 24 (T24), and 48 (T48) hr post-drug removal. Healthy subjects were also included. Baseline DNA damage was elevated in cancer patients (variability between individuals was observed). After cPt, patients showed increased γH2AX foci/nucleus (T24 and T48). Both in healthy persons and patients, the nuclear p-Hsp90α and N/C (nuclear/cytoplasmic) ratio augmented (T24), decreasing at T48. Favorable clinical response was associated with high DNA damage and p-Hsp90α N/C ratio following cPt. For the first time, p-Hsp90α significance as a predictive marker is highlighted. Post-cPt-DNA damage was associated with longer disease-free survival and overall survival. Our findings indicate that comet assay and p-Hsp90α (a marker of DDR) would be promising prognostic/predictive tools in cP-treated cancer patients.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10956442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140028224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acellular Extrinsic Fiber Cementum Is Invariably Present in the Superficial Layer of Apical Cementum in Mouse Molar. 小鼠臼齿根尖骨水泥膜表层始终存在细胞外纤维骨水泥膜

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-02-01 Epub Date: 2024-01-30 DOI: 10.1369/00221554241229130

Hajime Iwama, Masaru Kaku, Lay Thant, Masaru Mizukoshi, Moe Arai, Yoshiki Ono, Kohei Kitami, Isao Saito, Katsumi Uoshima

{"title":"Acellular Extrinsic Fiber Cementum Is Invariably Present in the Superficial Layer of Apical Cementum in Mouse Molar.","authors":"Hajime Iwama, Masaru Kaku, Lay Thant, Masaru Mizukoshi, Moe Arai, Yoshiki Ono, Kohei Kitami, Isao Saito, Katsumi Uoshima","doi":"10.1369/00221554241229130","DOIUrl":"10.1369/00221554241229130","url":null,"abstract":"The cementum is a highly mineralized tissue that covers the tooth root. The regional differences among the types of cementum, especially in the extrinsic fibers that contribute to tooth support, remain controversial. Therefore, this study used second harmonic generation imaging in conjunction with automated collagen extraction and image analysis algorithms to facilitate the quantitative examination of the fiber characteristics and the changes occurring in these fibers over time. Acellular extrinsic fiber cementum (AEFC) was invariably observed in the superficial layer of the apical cementum in mouse molars, indicating that this region of the cementum plays a crucial role in supporting the tooth. The apical AEFC exhibited continuity and fiber characteristics comparable with the cervical AEFC, suggesting a common cellular origin for their formation. The cellular intrinsic fiber cementum present in the inner layer of the apical cementum showed consistent growth in the apical direction without layering. This study highlights the dynamic nature of the cementum in mouse molars and underscores the requirement for re-examining its structure and roles. The findings of the present study elucidate the morphophysiological features of cementum and have broader implications for the maintenance of periodontal tissue health.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10851881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139575789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Terminal βGlcNAc on Brachyspira Species in Human Intestinal Spirochetosis. 人类肠道螺旋体病中 Brachyspira 菌种末端 βGlcNAc 的鉴定

IF 3.2 4区生物学

Journal of Histochemistry & Cytochemistry Pub Date : 2024-02-01 Epub Date: 2024-01-08 DOI: 10.1369/00221554231222963

Hisanori Matoba, Mai Iwaya, Chifumi Fujii, Jun Nakayama

{"title":"Identification of Terminal βGlcNAc on Brachyspira Species in Human Intestinal Spirochetosis.","authors":"Hisanori Matoba, Mai Iwaya, Chifumi Fujii, Jun Nakayama","doi":"10.1369/00221554231222963","DOIUrl":"10.1369/00221554231222963","url":null,"abstract":"Human intestinal spirochetosis (HIS) is a colorectal bacterial infection caused by the Brachyspira species. Griffonia simplicifolia-II (GS-II) is a lectin specific to terminal α/βGlcNAc residues. Here, we investigated terminal βGlcNAc residues in the context of HIS infection using GS-II-horseradish peroxidase staining and HIK1083 immunostaining specific to terminal αGlcNAc residues. Fourteen of 15 HIS cases were GS-II-positive on the bacterial body. No cases showed HIK1083 positivity. The percentage of bacterial bodies staining positively for GS-II based on comparison with anti-Treponema immunostaining was ≤30% in seven cases, 30-70% in two, and >70% in six. Of 15 HIS cases analyzed, none were comorbid with tubular adenomas, and three were comorbid with sessile serrated lesions (SSLs). To determine the species of spirochete infected, the B. aalborgi-specific or B. pilosicoli-specific NADPH oxidase genes were amplified by PCR. After direct sequencing of the PCR products, all nine cases in which PCR products were observed were found to be infected with B. aalborgi alone. These results indicate that the HIS bacterial body, especially of B. aalborgi, is characterized by terminal βGlcNAc and also indicate that terminal βGlcNAc on the HIS bacterial body is associated with HIS preference for SSLs.","PeriodicalId":16079,"journal":{"name":"Journal of Histochemistry & Cytochemistry","volume":null,"pages":null},"PeriodicalIF":3.2,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10851879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139377802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0