Journal of Ginseng Research最新文献

{"title":"The effects of Korean Red Ginseng on heme oxygenase-1 with a focus on mitochondrial function in pathophysiologic conditions","authors":"Chang-Hee Kim ,&nbsp;Hahn Young Kim ,&nbsp;Seung-Yeol Nah ,&nbsp;Yoon Kyung Choi","doi":"10.1016/j.jgr.2023.04.001","DOIUrl":"10.1016/j.jgr.2023.04.001","url":null,"abstract":"<div><p>Korean Red Ginseng (KRG) plays a key role in heme oxygenase (HO)-1 induction under physical and moderate oxidative stress conditions. The transient and mild induction of HO-1 is beneficial for cell protection, mitochondrial function, regeneration, and intercellular communication. However, chronic HO-1 overexpression is detrimental in severely injured regions. Thus, in a chronic pathological state, diminishing HO-1-mediated ferroptosis is beneficial for a therapeutic approach. The molecular mechanisms by which KRG protects various cell types in the central nervous system have not yet been established, especially in terms of HO-1-mediated mitochondrial functions. Therefore, in this review, we discuss the multiple roles of KRG in the regulation of astrocytic HO-1 under pathophysiological conditions. More specifically, we discuss the role of the KRG-mediated astrocytic HO-1 pathway in regulating mitochondrial functions in acute and chronic neurodegenerative diseases as well as physiological conditions.</p></div>","PeriodicalId":16035,"journal":{"name":"Journal of Ginseng Research","volume":"47 5","pages":"Pages 615-621"},"PeriodicalIF":6.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/38/a1/main.PMC10499582.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10308465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginsenoside Rk1 inhibits HeLa cell proliferation through an endoplasmic reticulum signaling pathway","authors":"Qiuyang Li ,&nbsp;Hang Sun ,&nbsp;Shiwei Liu,&nbsp;Jinxin Tang,&nbsp;Shengnan Liu,&nbsp;Pei Yin,&nbsp;Qianwen Mi,&nbsp;Jingsheng Liu,&nbsp;Lei yu,&nbsp;Yunfeng Bi","doi":"10.1016/j.jgr.2023.04.004","DOIUrl":"10.1016/j.jgr.2023.04.004","url":null,"abstract":"<div><h3>Background</h3><p>Changes to work-life balance has increased the incidence of cervical cancer among younger people. A minor ginseng saponin known as ginsenoside Rk1 can inhibit the growth and survival of human cancer cells; however, whether ginsenoside Rk1 inhibits HeLa cell proliferation is unknown.</p></div><div><h3>Methods and results</h3><p>Ginsenoside Rk1 blocked HeLa cells in the G0/G1 phase in a dose-dependent manner and inhibited cell division and proliferation. Ginsenoside Rk1 markedly also activated the apoptotic signaling pathway via caspase 3, PARP, and caspase 6. In addition, ginsenoside Rk1 increased LC3B protein expression, indicating the promotion of the autophagy signaling pathway. Protein processing in the endoplasmic reticulum signaling pathway was downregulated in Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses, consistent with teal-time quantitative PCR and western blotting that showed YOD1, HSPA4L, DNAJC3, and HSP90AA1 expression levels were dramatically decreased in HeLa cells treated with ginsenoside Rk1, with YOD1 was the most significantly inhibited by ginsenoside Rk1 treatment.</p></div><div><h3>Conclusion</h3><p>These findings indicate that the toxicity of ginsenoside Rk1 in HeLa cells can be explained by the inhibition of protein synthesis in the endoplasmic reticulum and enhanced apoptosis, with YOD1 acting as a potential target for cervical cancer treatment.</p></div>","PeriodicalId":16035,"journal":{"name":"Journal of Ginseng Research","volume":"47 5","pages":"Pages 645-653"},"PeriodicalIF":6.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/74/bf/main.PMC10499649.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10655895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential of Panax notoginseng against COVID-19 infection","authors":"Yeye Hu ,&nbsp;Ziliang He ,&nbsp;Wei Zhang ,&nbsp;Zhiqiang Niu ,&nbsp;Yanting Wang ,&nbsp;Ji Zhang ,&nbsp;Ting Shen ,&nbsp;Hong Cheng ,&nbsp;Weicheng Hu","doi":"10.1016/j.jgr.2023.04.002","DOIUrl":"https://doi.org/10.1016/j.jgr.2023.04.002","url":null,"abstract":"<div><p>The COVID-19 pandemic has changed the world and has presented the scientific community with unprecedented challenges. Infection is associated with overproduction of proinflammatory cytokines secondary to hyperactivation of the innate immune response, inducing a cytokine storm and triggering multiorgan failure and significant morbidity/mortality. No specific treatment is yet available. For thousands of years, <em>Panax notoginseng</em> has been used to treat various infectious diseases. Experimental evidence of <em>P.</em> notoginseng utility in terms of alleviating the cytokine storm, especially the cascade, and improving post-COVID-19 symptoms, suggests that <em>P.</em> notoginseng may serve as a valuable adjunct treatment for COVID-19 infection.</p></div>","PeriodicalId":16035,"journal":{"name":"Journal of Ginseng Research","volume":"47 5","pages":"Pages 622-626"},"PeriodicalIF":6.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50196372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of anti-skin disorders of ginsenosides- A Systematic Review","authors":"Lele Cong ,&nbsp;Jinli Ma ,&nbsp;Yundong Zhang ,&nbsp;Yifa Zhou ,&nbsp;Xianling Cong ,&nbsp;Miao Hao","doi":"10.1016/j.jgr.2023.04.005","DOIUrl":"10.1016/j.jgr.2023.04.005","url":null,"abstract":"<div><p>Ginsenosides are bioactive components of Panax ginseng with many functions such as anti-aging, anti-oxidation, anti-inflammatory, anti-fatigue, and anti-tumor. Ginsenosides are categorized into dammarane, oleanene, and ocotillol type tricyclic triterpenoids based on the aglycon structure. Based on the sugar moiety linked to C-3, C-20, and C-6, C-20, dammarane type was divided into protopanaxadiol (PPD) and protopanaxatriol (PPT). The effects of ginsenosides on skin disorders are noteworthy. They play anti-aging roles by enhancing immune function, resisting melanin formation, inhibiting oxidation, and elevating the concentration of collagen and hyaluronic acid. Thus, ginsenosides have previously been widely used to resist skin diseases and aging. This review details the role of ginsenosides in the anti-skin aging process from mechanisms and experimental research.</p></div>","PeriodicalId":16035,"journal":{"name":"Journal of Ginseng Research","volume":"47 5","pages":"Pages 605-614"},"PeriodicalIF":6.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/48/a7/main.PMC10499590.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10300439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery and validation of PURA as a transcription target of 20(S)-protopanaxadiol: Implications for the treatment of cognitive dysfunction","authors":"Feiyan Chen ,&nbsp;Wenjing Zhang ,&nbsp;Shuyi Xu ,&nbsp;Hantao Zhang ,&nbsp;Lin Chen ,&nbsp;Cuihua Chen ,&nbsp;Zhu Zhu ,&nbsp;Yunan Zhao","doi":"10.1016/j.jgr.2023.04.007","DOIUrl":"10.1016/j.jgr.2023.04.007","url":null,"abstract":"<div><h3>Background</h3><p>20(S)-protopanaxadiol (PPD), a ginsenoside metabolite, has prominent benefits for the central nervous system, especially in improving learning and memory. However, its transcriptional targets in brain tissue remain unknown.</p></div><div><h3>Methods</h3><p>In this study, we first used mass spectrometry-based drug affinity responsive target stability (DARTS) to identify the potential proteins of ginsenosides and intersected them with the transcription factor library. Second, the transcription factor PURA was confirmed as a target of PPD by biolayer interferometry (BLI) and molecular docking. Next, the effect of PPD on the transcriptional levels of target genes of PURA in brain tissues was determined by qRT-PCR. Finally, bioinformatics analysis was used to analyze the potential biological features of these target proteins.</p></div><div><h3>Results</h3><p>The results showed three overlapping transcription factors between the proteomics of DARTS and transcription factor library. BLI analysis further showed that PPD had a higher direct interaction with PURA than parent ginsenosides. Subsequently, BLI kinetic analysis, molecular docking, and mutations in key amino acids of PURA indicated that PPD specifically bound to PURA. The results of qRT-PCR showed that PPD could increase the transcription levels of PURA target genes in brain. Finally, bioinformatics analysis showed that these target proteins were involved in learning and memory function.</p></div><div><h3>Conclusion</h3><p>The above-mentioned findings indicate that PURA is a transcription target of PPD in brain, and PPD upregulate the transcription levels of target genes related to cognitive dysfunction by binding PURA, which could provide a chemical and biological basis for the study of treating cognitive impairment by targeting PURA.</p></div>","PeriodicalId":16035,"journal":{"name":"Journal of Ginseng Research","volume":"47 5","pages":"Pages 662-671"},"PeriodicalIF":6.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fa/e4/main.PMC10499581.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10308462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to ‘Effect of fermented red ginseng on gut microbiota dysbiosis- or immobilization stress-induced anxiety, depression, and colitis in mice’[Journal of Ginseng Research Volume 47, Issue 2, March 2023, Pages 255-264]","authors":"Yoon-Jung Shin ,&nbsp;Dong-Yun Lee ,&nbsp;Joo Yun Kim ,&nbsp;Keon Heo ,&nbsp;Jae-Jung Shim ,&nbsp;Jung-Lyoul Lee ,&nbsp;Dong-Hyun Kim","doi":"10.1016/j.jgr.2023.07.001","DOIUrl":"10.1016/j.jgr.2023.07.001","url":null,"abstract":"","PeriodicalId":16035,"journal":{"name":"Journal of Ginseng Research","volume":"47 5","pages":"Page 683"},"PeriodicalIF":6.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10499568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10287537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amelioration of colitis progression by ginseng-derived exosome-like nanoparticles through suppression of inflammatory cytokines","authors":"Jisu Kim ,&nbsp;Shuya Zhang ,&nbsp;Ying Zhu ,&nbsp;Ruirui Wang ,&nbsp;Jianxin Wang","doi":"10.1016/j.jgr.2023.01.004","DOIUrl":"10.1016/j.jgr.2023.01.004","url":null,"abstract":"<div><h3>Background</h3><p>Damage to the healthy intestinal epithelial layer and regulation of the intestinal immune system, closely interrelated, are considered pivotal parts of the curative treatment for inflammatory bowel disease (IBD). Plant-based diets and phytochemicals can support the immune microenvironment in the intestinal epithelial barrier for a balanced immune system by improving the intestinal microecological balance and may have therapeutic potential in colitis. However, there have been only a few reports on the therapeutic potential of plant-derived exosome-like nanoparticles (PENs) and the underlying mechanism in colitis. This study aimed to assess the therapeutic effect of PENs from <em>Panax ginseng</em>, ginseng-derived exosome-like nanoparticles (GENs), in a mouse model of IBD, with a focus on the intestinal immune microenvironment.</p></div><div><h3>Method</h3><p>To evaluate the anti-inflammatory effect of GENs on acute colitis, we treated GENs in Caco2 and lipopolysaccharide (LPS) -induced RAW 264.7 macrophages and analyzed the gene expression of pro-inflammatory cytokines and anti-inflammatory cytokines such as TNF-α, IL-6, and IL-10 by real-time PCR (RT-PCR). Furthermore, we further examined bacterial DNA from feces and determined the alteration of gut microbiota composition in DSS-induced colitis mice after administration of GENs through 16S rRNA gene sequencing analysis.</p></div><div><h3>Result</h3><p>GENs with low toxicity showed a long-lasting intestinal retention effect for 48 h, which could lead to effective suppression of pro-inflammatory cytokines such as TNF-α and IL-6 production through inhibition of NF-κB in DSS-induced colitis. As a result, it showed longer colon length and suppressed thickening of the colon wall in the mice treated with GENs. Due to the amelioration of the progression of DSS-induced colitis with GENs treatment, the prolonged survival rate was observed for 17 days compared to 9 days in the PBS-treated group. In the gut microbiota analysis, the ratio of Firmicutes/Bacteroidota was decreased, which means GENs have therapeutic effectiveness against IBD. Ingesting GENs would be expected to slow colitis progression, strengthen the gut microbiota, and maintain gut homeostasis by preventing bacterial dysbiosis.</p></div><div><h3>Conclusion</h3><p>GENs have a therapeutic effect on colitis through modulation of the intestinal microbiota and immune microenvironment. GENs not only ameliorate the inflammation in the damaged intestine by downregulating pro-inflammatory cytokines but also help balance the microbiota on the intestinal barrier and thereby improve the digestive system.</p></div>","PeriodicalId":16035,"journal":{"name":"Journal of Ginseng Research","volume":"47 5","pages":"Pages 627-637"},"PeriodicalIF":6.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1b/89/main.PMC10499592.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10300440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to’ Morphine dependence is attenuated by red ginseng extract and ginsenosides Rh2, Rg3, and compound K’ [Journal of Ginseng Research Volume 40, Issue 4, October 2016, Pages 445-452]","authors":"Taddesse Yayeh,&nbsp;Kyunghwa Yun,&nbsp;Soyong Jang,&nbsp;Seikwan Oh","doi":"10.1016/j.jgr.2023.06.002","DOIUrl":"10.1016/j.jgr.2023.06.002","url":null,"abstract":"","PeriodicalId":16035,"journal":{"name":"Journal of Ginseng Research","volume":"47 5","pages":"Pages 681-682"},"PeriodicalIF":6.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/eb/a0/main.PMC10499576.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10652905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The anti-platelet activity of panaxadiol fraction and panaxatriol fraction of Korean Red Ginseng in vitro and ex vivo","authors":"Yuan Yee Lee ,&nbsp;Yein Oh ,&nbsp;Min-Soo Seo ,&nbsp;Min-Goo Seo ,&nbsp;Jee Eun Han ,&nbsp;Kyoo-Tae Kim ,&nbsp;Jin-Kyu Park ,&nbsp;Sung Dae Kim ,&nbsp;Sang-Joon Park ,&nbsp;Dongmi Kwak ,&nbsp;Man Hee Rhee","doi":"10.1016/j.jgr.2023.03.003","DOIUrl":"10.1016/j.jgr.2023.03.003","url":null,"abstract":"<div><h3>Background</h3><p>The anti-platelet activity of the saponin fraction of Korean Red Ginseng has been widely studied. The saponin fraction consists of the panaxadiol fraction (PDF) and panaxatriol fraction (PTF); however, their anti-platelet activity is yet to be compared. Our study aimed to investigate the potency of anti-platelet activity of PDF and PTF and to elucidate how well they retain their anti-platelet activity via different administration routes.</p></div><div><h3>Methods</h3><p>For ex vivo studies, Sprague-Dawley rats were orally administered 250 mg/kg PDF and PTF for 7 consecutive days before blood collection via cardiac puncture. Platelet aggregation was conducted after isolation of the washed platelets. For in vitro studies, washed platelets were obtained from Sprague-Dawley rats. Collagen and adenosine diphosphate (ADP) were used to induce platelet aggregation. Collagen was used as an agonist for assaying adenosine triphosphate release, thromboxane B2, serotonin, cyclic adenosine monophosphate, and cyclic guanosine monophosphate (cGMP) release.</p></div><div><h3>Results</h3><p>When treated ex vivo, PDF not only inhibited ADP and collagen-induced platelet aggregation, but also upregulated cGMP levels and reduced platelet adhesion to fibronectin. Furthermore, it also inhibited Akt phosphorylation induced by collagen treatment. Panaxadiol fraction did not exert any anti-platelet activity in vitro, whereas PTF exhibited potent anti-platelet activity, inhibiting ADP, collagen, and thrombin-induced platelet aggregation, but significantly elevated levels of cGMP.</p></div><div><h3>Conclusion</h3><p>Our study showed that in vitro and ex vivo PDF and PTF treatments exhibited different potency levels, indicating possible metabolic conversions of ginsenosides, which altered the content of ginsenosides capable of preventing platelet aggregation.</p></div>","PeriodicalId":16035,"journal":{"name":"Journal of Ginseng Research","volume":"47 5","pages":"Pages 638-644"},"PeriodicalIF":6.3,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/07/67/main.PMC10499584.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10300441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ginsenosides Rg1 regulate lipid metabolism and temperature adaptation in Caenorhabditis elegans","authors":"Hao Shi ,&nbsp;Jiamin Zhao ,&nbsp;Yiwen Li ,&nbsp;Junjie Li ,&nbsp;Yunjia Li ,&nbsp;Jia Zhang ,&nbsp;Zhantu Qiu ,&nbsp;Chaofeng Wu ,&nbsp;Mengchen Qin ,&nbsp;Chang Liu ,&nbsp;Zhiyun Zeng ,&nbsp;Chao Zhang ,&nbsp;Lei Gao","doi":"10.1016/j.jgr.2022.11.005","DOIUrl":"10.1016/j.jgr.2022.11.005","url":null,"abstract":"<div><h3>Background</h3><p>Obesity is a risk factor for aging and many diseases, and the disorder of lipid metabolism makes it prominent. This study aims to investigate the effect of ginsenoside Rg1 on aging, lipid metabolism and stress resistance</p></div><div><h3>Methods</h3><p>Rg1 was administered to <em>Caenorhabditis elegans</em> (<em>C. elegans</em>) cultured in NGM or GNGM. The lifespan, locomotory activity, lipid accumulation, cold and heat stress resistance and related mRNA expression of the worms were examined. Gene knockout mutants were used to clarify the effect on lipid metabolism of Rg1. GFP-binding mutants were used to observe the changes in protein expression</p></div><div><h3>Results</h3><p>We reported that Rg1 reduced lipid accumulation and improved stress resistance in <em>C. elegans</em>. Rg1 significantly reduced the expression of fatty acid synthesis-related genes and lipid metabolism-related genes in <em>C. elegans</em>. However, Rg1 did not affect the fat storage in <em>fat-5/fat-6</em> double mutant or <em>nhr-49</em> mutant. Combined with network pharmacology, we clarified the possible pathways and targets of Rg1 in lipid metabolism. In addition, Rg1-treated <em>C. elegans</em> showed a higher expression of anti-oxidative genes and heat shock proteins, which might contribute to stress resistance</p></div><div><h3>Conclusion</h3><p>Rg1 reduced fat accumulation by regulating lipid metabolism via <em>nhr-49</em> and enhanced stress resistance by its antioxidant effect in <em>C. elegans</em>.</p></div>","PeriodicalId":16035,"journal":{"name":"Journal of Ginseng Research","volume":"47 4","pages":"Pages 524-533"},"PeriodicalIF":6.3,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0c/7f/main.PMC10310868.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10122332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}