Ginseng root-derived exosome-like nanoparticles protect skin from UV irradiation and oxidative stress by suppressing activator protein-1 signaling and limiting the generation of reactive oxygen species

IF 6.8 2区 医学 Q1 CHEMISTRY, MEDICINAL
Wooram Choi , Jeong Hun Cho , Sang Hee Park , Dong Seon Kim , Hwa Pyoung Lee , Donghyun Kim , Hyun Soo Kim , Ji Hye Kim , Jae Youl Cho
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Abstract

Background

Recently, plant-derived exosome-like nanoparticles (PDENs) have been isolated, and active research was focusing on understanding their properties and functions. In this study, the characteristics and molecular properties of ginseng root-derived exosome-like nanoparticles (GrDENs) were examined in terms of skin protection.

Methods

HPLC-MS protocols were used to analyze the ginsenoside contents in GrDENs. To investigate the beneficial effect of GrDENs on skin, HaCaT cells were pre-treated with GrDENs (0–2 × 109 particles/mL), and followed by UVB irradiation or H2O2 exposure. In addition, the antioxidant activity of GrDENs was measured using a fluorescence microscope or flow cytometry. Finally, molecular mechanisms were examined with immunoblotting analysis.

Results

GrDENs contained detectable levels of ginsenosides (Re, Rg1, Rb1, Rf, Rg2 (S), Gyp17, Rd, C-Mc1, C–O, and F2). In UVB-irradiated HaCaT cells, GrDENs protected cells from death and reduced ROS production. GrDENs downregulated the mRNA expression of proapoptotic genes, including BAX, caspase-1, -3, -6, -7, and -8 and the ratio of cleaved caspase-8, -9, and -3 in a dose-dependent manner. In addition, GrDENs reduced the mRNA levels of aging-related genes (MMP2 and 3), proinflammatory genes (COX-2 and IL-6), and cellular senescence biomarker p21, possibly by suppressing activator protein-1 signaling.

Conclusions

This study demonstrates the protective effects of GrDENs against skin damage caused by UV and oxidative stress, providing new insights into beneficial uses of ginseng. In particular, our results suggest GrDENs as a potential active ingredient in cosmeceuticals to promote skin health.

Abstract Image

Abstract Image

人参根提取的外泌体纳米颗粒通过抑制激活蛋白-1信号传导和限制活性氧的生成,保护皮肤免受紫外线照射和氧化应激的伤害
背景最近,植物源类外泌体纳米颗粒(PDENs)被分离出来,人们正在积极研究了解它们的特性和功能。本研究从保护皮肤的角度研究了人参根源性外泌体纳米颗粒(GrDENs)的特征和分子特性。用 GrDENs(0-2 × 109 颗粒/毫升)预处理 HaCaT 细胞,然后照射 UVB 或暴露于 H2O2。此外,还使用荧光显微镜或流式细胞仪测量了 GrDENs 的抗氧化活性。结果 GrDENs 含有可检测水平的人参皂苷(Re、Rg1、Rb1、Rf、Rg2 (S)、Gyp17、Rd、C-Mc1、C-O 和 F2)。在经 UVB 照射的 HaCaT 细胞中,GrDENs 可保护细胞免于死亡并减少 ROS 的产生。GrDENs 以剂量依赖的方式下调了促凋亡基因的 mRNA 表达,包括 BAX、caspase-1、-3、-6、-7 和 -8,以及裂解的 caspase-8、-9 和 -3 的比率。此外,GrDENs 还降低了衰老相关基因(MMP2 和 3)、促炎基因(COX-2 和 IL-6)以及细胞衰老生物标志物 p21 的 mRNA 水平,这可能是通过抑制激活蛋白-1 信号转导实现的。特别是,我们的研究结果表明 GrDENs 是药妆产品中促进皮肤健康的潜在活性成分。
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来源期刊
Journal of Ginseng Research
Journal of Ginseng Research CHEMISTRY, MEDICINAL-INTEGRATIVE & COMPLEMENTARY MEDICINE
CiteScore
11.40
自引率
9.50%
发文量
111
审稿时长
6-12 weeks
期刊介绍: Journal of Ginseng Research (JGR) is an official, open access journal of the Korean Society of Ginseng and is the only international journal publishing scholarly reports on ginseng research in the world. The journal is a bimonthly peer-reviewed publication featuring high-quality studies related to basic, pre-clinical, and clinical researches on ginseng to reflect recent progresses in ginseng research. JGR publishes papers, either experimental or theoretical, that advance our understanding of ginseng science, including plant sciences, biology, chemistry, pharmacology, toxicology, pharmacokinetics, veterinary medicine, biochemistry, manufacture, and clinical study of ginseng since 1976. It also includes the new paradigm of integrative research, covering alternative medicinal approaches. Article types considered for publication include review articles, original research articles, and brief reports. JGR helps researchers to understand mechanisms for traditional efficacy of ginseng and to put their clinical evidence together. It provides balanced information on basic science and clinical applications to researchers, manufacturers, practitioners, teachers, scholars, and medical doctors.
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