Journal of Histotechnology最新文献_第4页

Immunohistochemistry as an assay. 免疫组织化学作为一种检测方法。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-12-01 Epub Date: 2023-11-27 DOI: 10.1080/01478885.2023.2278384

Michelle Bell, Luis Chiriboga, Elizabeth Chlipala, Colleen Forster, Jeremy Johnston, Jerry Santiago, Dawn Schneider, Shameika J Winfrey, Brenda L Schlosser, Clare Thornton, Elba G Vidal

引用次数: 0

A customized 3D-printed histological microgrinder for the study of metallic endoprostheses. 一种用于研究金属内假体的定制3D打印组织学微研磨器。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-12-01 Epub Date: 2023-05-02 DOI: 10.1080/01478885.2023.2205617

Reza Sanaei

引用次数: 1

Protective effects of a natural product, paeoniflorin, on ischemia reperfusion injury on rat ovary tissue: histopathological, immunohistochemical, and biochemical study. 天然产物芍药苷对大鼠卵巢缺血再灌注损伤的保护作用:组织病理学、免疫组织化学和生化研究。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-12-01 Epub Date: 2023-06-23 DOI: 10.1080/01478885.2023.2227409

Pinar Bayram, Selina Aksak Karamese, Huseyin Serkan Erol, Bengul Ozdemir, Erdem Toktay, Cagatay Salum

{"title":"Protective effects of a natural product, paeoniflorin, on ischemia reperfusion injury on rat ovary tissue: histopathological, immunohistochemical, and biochemical study.","authors":"Pinar Bayram, Selina Aksak Karamese, Huseyin Serkan Erol, Bengul Ozdemir, Erdem Toktay, Cagatay Salum","doi":"10.1080/01478885.2023.2227409","DOIUrl":"10.1080/01478885.2023.2227409","url":null,"abstract":"In this study, the main hypothesis is that paeoniflorin may inhibit some cellular processes such as oxidative stress and inflammation. For this reason, we aimed to investigate the potential protective effects of a natural compound, paeoniflorin, on rat model of ovarian ischemia-reperfusion injury by detecting the oxidative stress parameters and inflammatory process parameters. 42 female Wistar-albino rats were divided into 6 random groups. The rats were subjected to 3-hour ischemia and 3-hour reperfusion process. Then, paeoniflorin at doses of 25, 50 and 100 mg/kg were applied 30 min before the reperfusion. The levels of pro-inflammatory (IL-1-β, IL-6, TNF-α) and anti-inflammatory (IL-10, TGF-β) cytokines were measured by ELISA. Similarly, IL-6, IL-10, TNF-α, NF-κB p65) positivity rates were detected by immunohistochemical staining. Additionally, oxidative stress parameters (MDA, GSH, SOD) were measured by tissue biochemistry. Ischemia-reperfusion injury caused significant increase in the levels of SOD, MDA, TNF-α, IL-1-β, IL-6 and NF-κB p65, while paeoniflorin treatments improved the related parameters in a dose-dependent manner. As a conclusion, our findings support the evidence that paeoniflorin has a potential protective effects on ovarian ischemia-reperfusion injury. Further detailed studies should be performed to shed light the molecular mechanism of these protective effects.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10035374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hematoxylin and Eosin staining of PhenoCycler® Fusion flow cell slides. PhenoCycler®Fusion流式细胞玻片的苏木精和伊红染色。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-12-01 Epub Date: 2023-08-16 DOI: 10.1080/01478885.2023.2245182

Tomoe Shiomi, Anna Eichinger, Luis Chiriboga

引用次数: 0

Exosomal miR-192-5p secreted by bone marrow mesenchymal stem cells inhibits hepatic stellate cell activation and targets PPP2R3A. 骨髓间充质干细胞分泌的外泌体miR-192-5p抑制肝星状细胞活化并靶向PPP2R3A。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-12-01 Epub Date: 2023-05-25 DOI: 10.1080/01478885.2023.2215151

Jie Tan, Mingtao Chen, Meng Liu, Aifang Chen, Min Huang, Xiaoli Chen, Xia Tian, Wei Chen

{"title":"Exosomal miR-192-5p secreted by bone marrow mesenchymal stem cells inhibits hepatic stellate cell activation and targets PPP2R3A.","authors":"Jie Tan, Mingtao Chen, Meng Liu, Aifang Chen, Min Huang, Xiaoli Chen, Xia Tian, Wei Chen","doi":"10.1080/01478885.2023.2215151","DOIUrl":"10.1080/01478885.2023.2215151","url":null,"abstract":"Bone marrow mesenchymal stem cell (BSMC)-derived extracellular vehicles (EVs) have a pivotal therapeutic potential in hepatic fibrosis (HF). Activation of hepatic stellate cells (HSCs) is the key mechanism in HF progression. Downregulation of miR-192-5p was previously observed in activated HSCs. Nonetheless, the functions of BSMC-derived exosomal miR-192-5p in activated HSCs remain unclear. In this study, transforming growth factor (TGF)-β1 was used to activate HSC-T6 cells to mimic HF in vitro. Characterization of BMSCs and BMSC-derived EVs was performed. Cell-counting kit-8 assay, flow cytometry, and western blotting revealed that TGF-β1 increased cell viability, promoted cell cycle progression, and induced upregulation of fibrosis markers in HSC-T6 cells. Overexpression of miR-192-5p or BMSC-derived exosomal miR-192-5p suppressed TGF-β1-triggered HSC-T6 cell activation. RT-qPCR revealed that protein phosphatase 2 regulatory subunit B'' alpha (PPP2R3A) was downregulated in miR-192-5p-overexpressed HSC-T6 cells. Luciferase reporter assay was used for verifying the relation between miR-192-5p and PPP2R3A, which showed that miR-192-5p targeted PPP2R3A in activated HSC-T6 cells. Collectively, BMSC-derived exosomal miR-192-5p targets PPP2R3A and inhibits activation of HSC-T6 cells.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9519041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of iron and ferroptosis in the pathogenesis of acute pancreatitis. 铁和脱铁症在急性胰腺炎发病机制中的作用。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-12-01 Epub Date: 2023-11-27 DOI: 10.1080/01478885.2023.2261093

Jin Tao, Yiyi Zhang, Yinshi Huang, Min Xu

{"title":"The role of iron and ferroptosis in the pathogenesis of acute pancreatitis.","authors":"Jin Tao, Yiyi Zhang, Yinshi Huang, Min Xu","doi":"10.1080/01478885.2023.2261093","DOIUrl":"10.1080/01478885.2023.2261093","url":null,"abstract":"Acute pancreatitis (AP) is an inflammatory disease of the pancreas. Iron is an essential element for life and is involved in many metabolic processes. Ferroptosis is a type of regulated cell death that is triggered by iron and oxidative stress. A well-established mouse AP model was adopted to study the role of iron and ferroptosis in the pathogenesis of pancreatitis. Mice were injected with cerulein to induce AP, and pancreatic tissue samples were analyzed to determine the pathology, cell death, iron deposition, expression of iron transporters, and lipid peroxidation. The role of iron was studied by giving mice extra iron or iron chelator. In vitro studies with acinar cells with ferroptosis activator and inhibitor were also performed to assess the inflammatory response. Iron was found accumulated in the pancreatic tissue of mice who suffered cerulein-induced pancreatitis. Cell death and lipid peroxidation increased in these tissues and could be further modulated by iron dextran or iron chelator. Mice given Hemin through gavage had reduced levels of GSH in pancreatic tissue and increased inflammatory response. Studies with acinar cells showed increased levels of lipid peroxidation and ferroptosis-specific mitochondrial damage when treated with ferroptosis inducer and inflammatory cytokines.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41203066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The burden of uterine fibroids: an overview. 子宫肌瘤的负担：综述。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-12-01 Epub Date: 2023-11-27 DOI: 10.1080/01478885.2023.2265185

Jitu W George

引用次数: 0

The thorax: medical, radiological and pathological assessment The thorax: medical, radiological and pathological assessment , edited by Moran, Cesar A., Truong, Mylene T., de Groot, Patricia M., Cham (Switzerland), Springer, 2023, 934 (+xvi) pp., $249.99 (hardcover), $189.00 (e-book), ISBN: 978-3-031-21039-6, ISBN: 978-3-031-21040-2 (e-book) 《胸腔:医学、放射学和病理学评估》，Moran, Cesar A.， Truong, Mylene T.， de Groot, Patricia M.， Cham(瑞士)主编，Springer出版社，2023年，934页(+xvi)， 249.99美元(精装)，189.00美元(电子书)，ISBN: 978-3-031-21039-6, ISBN: 978-3-031-21040-2(电子书)

4区生物学

Journal of Histotechnology Pub Date : 2023-10-13 DOI: 10.1080/01478885.2023.2268935

Stephen K. Lau

引用次数: 0

Clinicopathological significance of SOX9 and β-catenin expression in pre-neoadjuvant chemotherapy cases of osteosarcoma: molecular and immunohistochemical study. 骨肉瘤新辅助化疗前SOX9和β-catenin表达的临床病理意义：分子和免疫组织化学研究。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-09-01 Epub Date: 2023-04-04 DOI: 10.1080/01478885.2023.2193526

Sarah Ahmed Hassan, Amal Abdel Aziz Shabaan, Adel Refaat Ahmed, Yasmine Amr Issa, Shady Hassan Fadel, Bassma Mohamed El-Sabaa

{"title":"Clinicopathological significance of SOX9 and β-catenin expression in pre-neoadjuvant chemotherapy cases of osteosarcoma: molecular and immunohistochemical study.","authors":"Sarah Ahmed Hassan, Amal Abdel Aziz Shabaan, Adel Refaat Ahmed, Yasmine Amr Issa, Shady Hassan Fadel, Bassma Mohamed El-Sabaa","doi":"10.1080/01478885.2023.2193526","DOIUrl":"10.1080/01478885.2023.2193526","url":null,"abstract":"The molecular pathogenesis of osteosarcoma (OS), the most frequent primary malignant bone tumor of all age groups, is still obscure. Since multidrug chemotherapeutic regimens were introduced in the 1970s, survival rates have been stationary. The Wnt-β-catenin signaling cascade and SOX9 have a significant contribution to skeletal growth, development, and tumorigenesis. In the present work, an attempt was made to examine the role and clinicopathological significance of β-catenin and SOX9 in 46 cases of pre-neoadjuvant chemotherapy OS tissues compared to 10 cases of non-neoplastic bone. The mRNA levels of both markers were assessed by qRT-PCR, and protein levels of β-catenin were analyzed by immunohistochemistry. The results were correlated with different clinicopathological parameters. SOX9 mRNA levels were significantly elevated in OS compared to non-neoplastic bone, and higher levels were significantly associated with the occurrence of fluid-fluid levels (indicating blood-containing cystic spaces) and osteolytic radiological pattern. Although β-catenin mRNA and protein levels were higher in OS compared to non-neoplastic bone, only the protein levels reached statistical significance. Higher β-catenin mRNA levels were significantly associated with tumor size, while higher protein levels were significantly associated with the histologic subtype, mitotic count, and radiological pattern. No significant association was noted with any of the other evaluated parameters. OS showing higher SOX9 mRNA expression and lower β-catenin mRNA and protein expression exhibited longer estimated overall survival times approaching statistical significance. To conclude, while high expression of β-catenin and SOX9 suggests their possible involvement in OS development, their prognostic role may need further research.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10203904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histologic examination of canned cat food. 罐头猫粮的组织学检查。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-09-01 Epub Date: 2023-02-22 DOI: 10.1080/01478885.2023.2177815

Levi Haven, Amanda Bodkin, Sheila L Criswell

{"title":"Histologic examination of canned cat food.","authors":"Levi Haven, Amanda Bodkin, Sheila L Criswell","doi":"10.1080/01478885.2023.2177815","DOIUrl":"10.1080/01478885.2023.2177815","url":null,"abstract":"Cat food production is a billion-dollar industry in the United States, with most pet owners trusting pet food companies to provide their pets with complete nutrition. Moist or canned cat food is healthier than dry kibble for cats due to its higher water content promoting healthy kidney function, but ingredient labels on canned cat food are lengthy with ambiguous terminology including 'animal by-products.' Forty canned cat food samples were collected from grocery stores and were processed using routine histologic methods. Hematoxylin and eosin-stained tissue sections were evaluated microscopically to determine the cat food content. Many brands and flavors were composed of well-preserved skeletal muscles admixed with various animal organs, which closely approximates nutritional components found in natural feline prey. However, several samples demonstrated marked degenerative changes suggesting a delay in food processing and potential decrease in nutrient content. Four samples contained cuts consisting of skeletal muscle only with no organ meat. Surprisingly, 10 samples contained fungal spores and 15 demonstrated refractile particulate matter. A cost analysis demonstrated that although the overall quality of canned cat food increases as the average cost per ounce increases, low-cost high-quality canned cat food is available.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10431707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0