Journal of Histotechnology最新文献_第4页

Automatic cell classification and quantification with machine learning in immunohistochemistry images. 免疫组织化学图像中的机器学习自动细胞分类和定量。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2026-03-01 Epub Date: 2025-07-01 DOI: 10.1080/01478885.2025.2523618

Pikting Cheung, Wei Zhang, Muhammad Shehzad Khan, Irfan Ahmed, Yuanchao Liu, Fraser Hill, Xinyue Li, Condon Lau

{"title":"Automatic cell classification and quantification with machine learning in immunohistochemistry images.","authors":"Pikting Cheung, Wei Zhang, Muhammad Shehzad Khan, Irfan Ahmed, Yuanchao Liu, Fraser Hill, Xinyue Li, Condon Lau","doi":"10.1080/01478885.2025.2523618","DOIUrl":"10.1080/01478885.2025.2523618","url":null,"abstract":"The incidence of lymphoma, a cancer that affects both humans and animals, has witnessed a significant increase. In response, immunohistochemistry (IHC) has become an essential tool for its classification. This prompted us to develop an innovative mathematical methodology for the precise quantification of immunopositive and immunonegative cells, along with their spatial analysis, in CD3-stained lymphoma IHC images. Our approach involves integrating an algorithm based on a mathematical color model for cell differentiation, employing the distinctive morphological erosion, algorithmic transformations, and customized histogram equalization to enhance features. Refined local thresholding enhances classification precision. Additionally, a customized circular Hough transform quantifies cell counts and assesses their spatial data. The algorithms accurately enumerate cell types, reducing human intervention and providing total numbers and spatial information on detected cells within tissue specimens. Evaluation of IHC image samples revealed an overall accuracy of 93.98% for automatic cell counts. The automatic counts and location information were cross-validated by three pathology specialists, highlighting the effectiveness and reliability of our automated approach. Our innovative framework enhances lymphoma cell counting accuracy in IHC images by combining physics-based color understanding with machine learning, thereby improving diagnosis and reducing the risks of human error.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"26-43"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of decalcification solutions on cellular morphology and immunostaining of mouse bones. 脱钙溶液对小鼠骨细胞形态及免疫染色的影响。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-12-01 Epub Date: 2025-11-12 DOI: 10.1080/01478885.2025.2583646

Madison Toonder, Skyler R Turner, Rachel Howie, Avi Barlatier, Miranda K Wilkes, Cindy Lowe, Tzushan S Yang, Katherine N Gibson-Corley

{"title":"Assessment of decalcification solutions on cellular morphology and immunostaining of mouse bones.","authors":"Madison Toonder, Skyler R Turner, Rachel Howie, Avi Barlatier, Miranda K Wilkes, Cindy Lowe, Tzushan S Yang, Katherine N Gibson-Corley","doi":"10.1080/01478885.2025.2583646","DOIUrl":"10.1080/01478885.2025.2583646","url":null,"abstract":"Decalcification is an important step in histology laboratories to allow mineralized tissue samples to be trimmed and sectioned easily. Many decalcifying solutions have a rapid onset of action in softening tissues but alter protein structure and morphology, while others preserve protein integrity but are less efficient. The ideal decalcification protocol allows for rapid and cost-effective processing and precise evaluation of microscopy and antigen-based immunostaining such as immunohistochemistry. In our study, mouse tissues were decalcified with three commercially available solutions to identify the product that best meets those criteria. ImmunocalTM (StatLab), EprediaTM, and Rapid-CalTM (StatLab) decalcification agents were tested on formalin-fixed, paraffin-embedded CD-1 mouse femur, skull, and sternum samples. Multiple metrics including ammonium oxalate turbidity and radiography were used to assess stages of bone demineralization. Tissues were routinely processed, embedded in paraffin, and sectioned at 5 µm for H&E staining and CD3, CD31, and Iba1 immunostaining. Each tested sample represented a decalcification product and time (4, 6, 12, 24, 30, 48, 73 h). Samples were assessed by radiolucency on X-ray and gross bone pliability prior to histologic processing, followed by histopathologic scoring for completeness of demineralization, preservation of tissue architecture, and antigenicity of tissue. All three commercially available decalcifying solutions are sufficient for rapid decalcification with preservation of tissue integrity, cellular detail, and immunogenicity.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"168-177"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145495636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathological analysis of Artemia franciscana nauplii under different forms of mercury stress. 不同形式汞胁迫下金翅蒿的组织病理学分析。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-12-01 Epub Date: 2025-07-02 DOI: 10.1080/01478885.2025.2523622

Yanlin Wei, Wei Liu, Fengling Zhang, Yang Chen, Dongxin Wei, Hangyu Lin, Tao He

{"title":"Histopathological analysis of Artemia franciscana nauplii under different forms of mercury stress.","authors":"Yanlin Wei, Wei Liu, Fengling Zhang, Yang Chen, Dongxin Wei, Hangyu Lin, Tao He","doi":"10.1080/01478885.2025.2523622","DOIUrl":"10.1080/01478885.2025.2523622","url":null,"abstract":"Inorganic mercury (Hg) and methylmercury (MeHg) have emerged as global pollutants owing to their long-term environmental stability and bioaccumulation. These heavy metals enter aquatic systems via industrial emissions, coal combustion, and natural processes, posing a serious threat to ecosystems and human health. This study assesses the impact of Hg2+ and MeHg on the growth and development of brine shrimp (Artemia franciscana) nauplii by analyzing the histopathological effects on their tissues. Brine shrimp nauplii from the Bohai Bay in China were selected as the study subjects and exposed to 1 μmol/L solutions of HgCl₂ and MeHgCl. Tissue sections were continuously taken at different immersion times. After staining with the standard hematoxylin-eosin (HE) method, the tissue morphology of brine shrimp nauplii under different forms of Hg stress was observed under a light microscope. The results showed that MeHg exhibited significantly greater toxicity to brine shrimp nauplii than Hg2+. Under the same exposure time, the MeHg group exhibited more pronounced epithelial cell damage, nuclear material disorder, and nucleoplasm diffusion outside the nucleus than the HgCl₂ group. This finding provides an important theoretical support for further research into the toxicological mechanisms of MeHg and Hg2+, and highlights that the toxic effects of methylmercury on aquatic organisms.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"178-190"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive panel of testing for amyloidosis. 淀粉样变性的综合检查。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-12-01 Epub Date: 2025-09-10 DOI: 10.1080/01478885.2025.2553926

Hong Tang, Feifei Zhao, Yingwei Zhu, Rongrong Xu, Huiqing Yuan, Min Xie, Rui Wu

{"title":"A comprehensive panel of testing for amyloidosis.","authors":"Hong Tang, Feifei Zhao, Yingwei Zhu, Rongrong Xu, Huiqing Yuan, Min Xie, Rui Wu","doi":"10.1080/01478885.2025.2553926","DOIUrl":"10.1080/01478885.2025.2553926","url":null,"abstract":"Amyloidosis encompasses a spectrum of rare disorders characterized by extracellular amyloid deposition. Achieving an accurate early diagnosis of systemic amyloidosis necessitates biopsy-specific pathological evaluation. Formalin-fixed, paraffin-embedded liver biopsy specimens were examined using Congo red staining, electron microscopy, immunohistochemistry (IHC), immunofluorescence, and Congo red-assisted laser microdissection with mass spectrometry (LMD/MS). Failure Mode and Effects Analysis (FMEA) was employed for risk mitigation and quality control. Classical Congo red staining exhibited brick-red coloration, enhanced alkalinization, reduced permanganate staining, and characteristic apple-green birefringence under polarized light. Trypsin-digested IHC demonstrated kappa light chain positivity and lambda negativity, with improved background clarity compared to other retrieval methods-results concordant with electron microscopic colloidal gold staining, albeit with higher tissue consumption. Congo red-polarized microscopy permitted direct amyloid deposit localization. Subsequent LMD/MS identified immunoglobulin kappa light chain as the pathogenic precursor protein, though at increased expense. Congo red staining under polarized light remains the cornerstone technique for amyloid detection. LMD/MS provides superior specificity in amyloid typing relative to IHC, immunofluorescence, or electron microscopy, proving particularly advantageous for limited samples. Traditional methods remain valuable for validation when tissue is abundant. Histopathological assessment continues to be the diagnostic gold standard for hepatic amyloidosis; systematic integration and analytical refinement of these techniques are imperative for enhancing diagnostic accuracy.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"191-199"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chinese expert opinion on AI-based identification of lymph node metastases in solid tumors. 基于人工智能识别实体瘤淋巴结转移的中国专家意见。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-12-01 Epub Date: 2025-11-10 DOI: 10.1080/01478885.2025.2580157

Xiaodong Wang, Hong Tang, Ying Chen, Xiaolin Zhang, Xiyang Liu, Minghua Zhu, Canrong Ni, Guanzhen Yu

引用次数: 0

Calcification density in single and twin placenta villi. 单胎座绒毛和双胎座绒毛钙化密度。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-12-01 Epub Date: 2025-09-10 DOI: 10.1080/01478885.2025.2557645

Maggie Guess, Sheila Criswell

{"title":"Calcification density in single and twin placenta villi.","authors":"Maggie Guess, Sheila Criswell","doi":"10.1080/01478885.2025.2557645","DOIUrl":"10.1080/01478885.2025.2557645","url":null,"abstract":"Placentas are temporary organs needed to support a developing embryo and arise from both embryonic and maternal tissues. Calcifications of tissues outside of bone and teeth mineralization are often a sign of tissue damage and impaired organ function. Placental calcifications have been described previously in the literature and usually increase in normal pregnancies as the placenta ages, but they have also been associated with the potential for fetal distress. This study utilized 139 placental tissues from singleton control placentas (51), fused twin placentas (48), and non-fused twin placentas (40) with weights over 320 grams (third trimester) and similar maternal ages to determine which placental type(s) exhibited the largest density of calcifications using the von Kossa stain for calcium salts. The study found there were no differences in calcification densities among placenta types, suggesting that twin pregnancies do not experience additional placental stress risk from calcifications. Importantly, the finding that nearly all third-trimester placentas contained calcifications when systematically evaluated indicates that calcification may represent a normal maturational process rather than a pathologic sign of distress. These results help to caution the interpretation of placental calcifications and may provide reassurance to patients and providers managing twin pregnancies.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"200-207"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiple levels of Gomori methenamine silver (GMS) stains do not improve diagnostic yield in esophageal biopsies. 高水平的戈莫里甲基苯丙胺银（GMS）染色不能提高食管活检的诊断率。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-12-01 Epub Date: 2024-12-12 DOI: 10.1080/01478885.2024.2437587

Michael Occidental, Andrew Dunn, Aaron R Huber

引用次数: 0

Corticoclaustral connections in the cat. 猫的皮质闭侧连接。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-09-01 Epub Date: 2025-03-14 DOI: 10.1080/01478885.2025.2476835

B Landzhov, D Hinova-Palova, K Fakih, L Edelstein, L Gaydarski, A Alexandrov, V Kirkov, A Paloff, E Radeva

{"title":"Corticoclaustral connections in the cat.","authors":"B Landzhov, D Hinova-Palova, K Fakih, L Edelstein, L Gaydarski, A Alexandrov, V Kirkov, A Paloff, E Radeva","doi":"10.1080/01478885.2025.2476835","DOIUrl":"10.1080/01478885.2025.2476835","url":null,"abstract":"The claustrum is a sheet-like layer of gray matter situated between the external and extreme capsules of the mammalian brain. This structure was first described by the French physician and anatomist Vicq d'Azyr in 1786. The claustrum's phylogeny, ontogeny and functional characteristics have long been the subject of debate and considerable investigative efforts. However, despite such efforts (or perhaps as a result thereof), significant disparities and discrepancies remain, most notably in the context of the claustrum's afferent and efferent connections. For the purpose of this study, we sought to focus our efforts on fronto-claustral and occipito-claustral connections. Twelve healthy, adult male cats, each weighing ~ 3.5 kg, were studied, seven of which underwent electrolytic lesions of the frontal cortex (A3, A4, and a portion of A6), and five of the occipital cortex (A17, A18, A21). From three to six days after lesioning, subjects were euthanized in accordance with ethical norms. After the brains were removed and blocked, samples of the claustrum were taken and prepared for electron microscopy. Three to six days after lesions of the frontal cortex, we observed an abundance of degenerative boutons in the dorsal claustrum. The vast majority of boutons exhibited the characteristic signs of dark degeneration, whereas only 10% appeared to have undergone light degeneration. Similar results were seen in the dorsal claustrum over the same period of time following lesions of the visual cortex. These results suggest that the dorsal claustrum receives at least two types of connections - separately and concurrently - from the frontal and occipital cortices.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"146-156"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilizing image analysis by optical density to evaluate changes in hematoxylin and eosin staining quality after reagent overuse. 利用光密度图像分析评价试剂过度使用后苏木精和伊红染色质量的变化。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-09-01 Epub Date: 2025-06-23 DOI: 10.1080/01478885.2025.2517906

Hannah M Benton, Mark Butters, Miles Brous, Brad Bolon, Karen Copeland, Jessica S Fortin, Elizabeth Chlipala

{"title":"Utilizing image analysis by optical density to evaluate changes in hematoxylin and eosin staining quality after reagent overuse.","authors":"Hannah M Benton, Mark Butters, Miles Brous, Brad Bolon, Karen Copeland, Jessica S Fortin, Elizabeth Chlipala","doi":"10.1080/01478885.2025.2517906","DOIUrl":"10.1080/01478885.2025.2517906","url":null,"abstract":"Due to the prevalence of hematoxylin and eosin (H&E) staining in routine histological preparations, understanding the factors that impact stain color characteristics is vital to attain consistently high-quality stains. In the last decade, increased use of digital pathology and image analysis (specifically by optical density [OD] measures) has provided new ways of assessing staining precision. This paper combines data from two studies that tracked H&E staining quality in both nuclear and cytoplasmic components of 12 tissues (11 human and 1 porcine) by OD after overuse of H&E staining reagents from 5 vendors. Both studies showed a decrease in eosin stain intensity by OD and visual inspection (by a histologist) with reagent overuse. This trend varied in degree by tissue type and reagent vendor. Nonetheless, staining quality for H&E staining from all vendors and for all organs remained acceptable (but not always optimal) for microscopic evaluation by the College of American Pathologists (CAP) and National Society for Histotechnology (NSH) staining criteria when stained sections were reviewed by a board-certified veterinary pathologist.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"123-134"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing tissue adherence on glass slides using polyurethane glue: a new slide preparation method. 利用聚氨酯胶优化组织粘附在玻片上：一种新的玻片制备方法。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-09-01 Epub Date: 2024-12-17 DOI: 10.1080/01478885.2024.2440679

Jeffery T Williams, Tracy A Goodpaster, Miki Haraguchi

引用次数: 0