Journal of Histotechnology最新文献_第3页

Morphometric study of bioprinted hydrogel deformation during histological preparation. 生物打印水凝胶在组织学制备过程中变形的形态计量学研究。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2026-06-01 Epub Date: 2025-09-09 DOI: 10.1080/01478885.2025.2539624

Lucie Essayan, Alexandre Dufour, Emma Petiot, Christophe Marquette

{"title":"Morphometric study of bioprinted hydrogel deformation during histological preparation.","authors":"Lucie Essayan, Alexandre Dufour, Emma Petiot, Christophe Marquette","doi":"10.1080/01478885.2025.2539624","DOIUrl":"10.1080/01478885.2025.2539624","url":null,"abstract":"Histological preparation via paraffin embedding is the gold standard method for evaluating tissue structure and composition, whether it is originated from biopsy or engineered in vitro. Quite often, deformation and shrinkage occur during the histological preparation, which are difficult to predict and qualify. The present study investigates the morphometric changes in bioprinted hydrogels composed of alginate and gelatine, common tissue engineering materials, focusing on three morphologies: full slabs, porous slabs, and porous cubes. These structures underwent key histological steps, including fixation, processing (dehydration, clearing, and infiltration with melted paraffin), embedding, and slicing, to evaluate their shrinkage behavior. Shrinking factors were systematically measured, showing that processing had the most significant effect (34-40% shrinking), followed by fixation (20-28% shrinking). Porous structures exhibited greater shrinkage compared to full slabs due to their internal geometry. Additionally, anisotropic behavior was observed in porous cubes, with different shrinking factors in the XY plane (horizontal) and Z direction (vertical), leading to an overall volumetric shrinking factor of 81.3%. The results demonstrated the critical influence of hydrogel structure on deformation and emphasized the need for tailored histological protocols to maintain structural fidelity. While this study focused on hydrogels alone, future work will incorporate cellularized bioengineered tissues to evaluate the impact of cell-mediated remodeling and extracellular matrix deposition on histological outcomes. This research offers a framework for optimizing histological preparation in bioengineered tissues, enabling more accurate assessment of their structure and function for regenerative medicine applications.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"88-98"},"PeriodicalIF":1.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

USP34 modulates mitochondrial function in triple-negative breast cancer cells through the eIf3m/MTCH2 axis. USP34通过eIf3m/MTCH2轴调节三阴性乳腺癌细胞的线粒体功能。

IF 1.9 4区生物学

Journal of Histotechnology Pub Date : 2026-06-01 Epub Date: 2026-04-23 DOI: 10.1080/01478885.2026.2648740

Peng-Fei Qian, Yi Zeng, Wang-Jing Zhong

{"title":"USP34 modulates mitochondrial function in triple-negative breast cancer cells through the eIf3m/MTCH2 axis.","authors":"Peng-Fei Qian, Yi Zeng, Wang-Jing Zhong","doi":"10.1080/01478885.2026.2648740","DOIUrl":"10.1080/01478885.2026.2648740","url":null,"abstract":"Triple-negative breast cancer (TNBC) represents a particularly aggressive form of breast tumors. Mitochondrial dysfunction represses the proliferation of TNBC cells. Ubiquitin-specific proteases 34 (USP34) has been predicted to be abnormally overexpressed in TNBC. This research examined the role of USP34 in the mitochondrial function modulation of TNBC. Herein, cell proliferation was evaluated by the 5-ethynyl-2'-deoxyuridine assay. Mitochondrial membrane potential was detected employing the JC-1 assay. Mitochondrial superoxide was measured utilizing MitoSOX Red assay. Mito‑Tracker Red CMXRos staining was selected to monitor mitochondrial network structure. The relationship among USP34, eukaryotic translation initiation factor 3 m (eIF3m), and mitochondrial carrier homolog 2 (MTCH2) was validated by co-immunoprecipitation, GST-pull down, RNA immunoprecipitation and RNA-pull down analysis. We found that USP34 silencing inhibited cell proliferation by inducing mitochondrial dysfunction in TNBC cells. USP34 maintained the stability of the eIF3m protein through deubiquitination. Overexpression of eIF3m countered the mitochondrial dysfunction induced by USP34 silencing. Furthermore, eIF3m upregulated the MTCH2 level by directly binding to its 5'UTR region. MTCH2 overexpression reversed the damaging effect of eIF3m silencing on mitochondrial function. Collectively, USP34 maintained the stability of eIF3m protein through deubiquitination; the upregulated eIF3m bound to the 5'UTR of MTCH2 mRNA to promote MTCH2 expression, thereby maintaining mitochondrial function and promoting the malignant progression of TNBC.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"113-125"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A look at scanner introduced variation in contrast, resolution, and colour across nine different models of whole slide imaging (WSI) scanner. 介绍了9种不同型号的全幻灯片成像（WSI）扫描仪在对比度、分辨率和颜色上的差异。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2026-04-21 DOI: 10.1080/01478885.2026.2643973

Hayley Pye, David S Brettle, Danny Kaye, Catriona M Dunn, Matthew P Humphries, Darren Treanor

引用次数: 0

Application of an open-source AI tool for quantitative quality control in whole slide images of prostate needle core biopsies - a retrospective study. 应用开源人工智能工具对前列腺穿刺活检全切片图像进行定量质量控制——回顾性研究。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2026-04-17 DOI: 10.1080/01478885.2026.2657106

Gonçalo Borrecho, Luís Rato, Pedro Salgueiro, Inês Ferreira, Catarina Madeira, Rui Caetano de Oliveira

{"title":"Application of an open-source AI tool for quantitative quality control in whole slide images of prostate needle core biopsies - a retrospective study.","authors":"Gonçalo Borrecho, Luís Rato, Pedro Salgueiro, Inês Ferreira, Catarina Madeira, Rui Caetano de Oliveira","doi":"10.1080/01478885.2026.2657106","DOIUrl":"https://doi.org/10.1080/01478885.2026.2657106","url":null,"abstract":"The quality of Whole Slide Images (WSI) is a determining factor for proper diagnosis and prognosis, and for enhanced performance of Digital and Computational Pathology. In a context where diagnoses are increasingly quantitative, an automated, precise, effective, and rapid quality control is of paramount importance. PathProfiler is a deep learning-based software trained on prostatic tissue that provides a 'usability score' of WSI, evaluating its suitability for diagnosis. The Centro de Anatomia Patológica Germano de Sousa receives around 2500 prostate biopsies a year, distributed to Pathologists remotely. Hence, it becomes crucial to investigate PathProfiler's viability for automated and quantitative WSI quality control and its monitoring for diagnostic purposes. Thus, in the last 3 months of 2024, 226 H&E WSI from prostate needle core biopsies were retrospectively analysed by PathProfiler. Usability score, focus, and H&E quality were registered numerically. WSIs had a usability score mean of 0.6, focus score of 9.6, and H&E quality score of 9.1. A usability score of 0.5 or higher was obtained for 70% of the WSIs, while 30% required revision. Artefacts such as mounting media, dust, and folded tissue were the major artifacts detected; extra-prostatic tissue was also recognised as 'other artifacts,' since the algorithm provides a lower rating in our cohort. PathProfiler is a valuable tool in the automatic quality control of prostate biopsies, allowing quick evaluation and identification of cases requiring review before being handed over to the pathologist, and promotes recognition of opportunities to improve laboratory and clinical quality.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":1.8,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147698783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Color standardization in whole slide imaging: a method to reduce color variability. 全幻灯片成像中的色彩标准化：一种减少色彩变异性的方法。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2026-03-11 DOI: 10.1080/01478885.2026.2636401

Louise Collins, Richard Heale, Richard M Salmon

{"title":"Color standardization in whole slide imaging: a method to reduce color variability.","authors":"Louise Collins, Richard Heale, Richard M Salmon","doi":"10.1080/01478885.2026.2636401","DOIUrl":"https://doi.org/10.1080/01478885.2026.2636401","url":null,"abstract":"Color standardization in digital pathology is essential for ensuring consistency across Whole Slide Imaging (WSI) scanners. This study utilizes color data from multiple WSI scanners captured from a slide containing 55 color patches with histology stain spectral characteristics alongside a proprietary algorithm to generate International Color Consortium (ICC) profiles. By transforming scanner-derived color values to align with their true spectral counterparts, the method enables effective color standardization. The color gamut of various scanner models was compared to the true spectral values both before and after standardization, as well as to the standard Red, Green and Blue (sRGB) gamut commonly used in display technologies. Results show that WSI scanners apply significantly different linear and tone curve profiles, as well as introducing variability in white and black point calibration. The proposed ICC-based standardization method substantially reduced discrepancies between scanned color values and their true spectral equivalents. This approach demonstrates the potential to mitigate scanner-induced color variability within digital pathology workflows. As artificial intelligence becomes increasingly integrated into pathology, addressing color presentation discrepancies between human observers and machine learning algorithms is critical. Application of standardized calibration would ensure consistency of data used for accurate diagnostic methods leading to reliable use for scaling of digital pathology. The uniquely large dataset presented here reveals how different image sources can generate significantly different outputs. Industry best practice would be to have all scanners calibrated by the same standard method and quantifiable accuracy, leading to real standardization rather than the variety of approaches this paper has revealed.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":1.8,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147433342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond algorithmic performance: translational gaps in implementing artificial intelligence for clinical digital pathology. 超越算法性能：临床数字病理学实施人工智能的翻译差距。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2026-03-01 Epub Date: 2026-02-23 DOI: 10.1080/01478885.2026.2630525

Rong Xia, Nickolas Littlefield, Riyue Bao, Qiangqiang Gu

引用次数: 0

Enhancing digital pathology workflows: computational blur detection for H&E image quality control in preclinical toxicology. 增强数字病理学工作流程：临床前毒理学中H&E图像质量控制的计算模糊检测。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2026-03-01 Epub Date: 2025-12-03 DOI: 10.1080/01478885.2025.2585615

Cyrus Manuel, Andy Cheon, Trung Nguyen, Sertan Kaya, Fangyao Hu, Ruth Sullivan, Reina Fuji

{"title":"Enhancing digital pathology workflows: computational blur detection for H&E image quality control in preclinical toxicology.","authors":"Cyrus Manuel, Andy Cheon, Trung Nguyen, Sertan Kaya, Fangyao Hu, Ruth Sullivan, Reina Fuji","doi":"10.1080/01478885.2025.2585615","DOIUrl":"10.1080/01478885.2025.2585615","url":null,"abstract":"Toxicologic pathology is undergoing a digital transformation, with advances in imaging and computational methods enabling automation of traditionally manual workflows. Central to these digital workflows is the generation of high-quality whole slide images (WSIs), where one key determinant of image quality is focus sharpness. To address this, we have integrated a pair of productionalized computational models - 'MiQC' (Microscopic Quality Control) - into our routine image QC workflows. MiQC combines Local Binary Patterns (LBP) and DeepFocus-based deep learning algorithms to detect and quantify out-of-focus regions in WSIs. Subsequent to scanner-based focus metric assessment, MiQC further screens WSIs and supports technician review by generating heatmaps that highlight problematic areas. Even WSIs with scanner focus scores of 98-99% can contain unacceptable blur, which MiQC helps identify. Using this system, 85-95% of WSIs are approved without further intervention, and technician review time is reduced by nearly 50%. Compared to fully manual review, MiQC has doubled our throughput of QC'd slides per hour. This efficiency gain has accelerated the expansion of our high-quality WSI repository and provides a scalable, reproducible framework for enhancing image QC in toxicologic and broader digital pathology applications. MiQC supports higher throughput and integration of automated image analysis pipelines, laying the groundwork for robust downstream computational pathology workflows.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"44-52"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145668757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphometric analysis and its application in lupus nephritis: a systematic review. 形态计量学分析及其在狼疮性肾炎中的应用综述。

IF 1.9 4区生物学

Journal of Histotechnology Pub Date : 2026-03-01 Epub Date: 2026-01-19 DOI: 10.1080/01478885.2025.2611623

Lucia Mercedes Niño-Hernandez, Liria Terradez Mas, Amparo Ruiz Sauri

{"title":"Morphometric analysis and its application in lupus nephritis: a systematic review.","authors":"Lucia Mercedes Niño-Hernandez, Liria Terradez Mas, Amparo Ruiz Sauri","doi":"10.1080/01478885.2025.2611623","DOIUrl":"10.1080/01478885.2025.2611623","url":null,"abstract":"Lupus nephritis (LN) is a severe manifestation of systemic lupus erythematosus (SLE), characterized by marked histological heterogeneity and high interobserver variability in the evaluation of renal biopsies. Digital morphometry has emerged as an objective and reproducible tool that enables precise quantification of tissuelesions, thereby enhancing diagnostic accuracy and prognostic assessment. To explore its application in LN, a systematic review was conducted following PRISMA guidelines, including observational studies in adults, children, and animal models with biopsy-proven LN in which digital morphometry was applied. Searches were performed inPubMed, Embase, Scopus, Web of Science, Cochrane, and Google Scholar. From 376 identified records, 46 studies fulfilled inclusion criteria. Among these, 30 analyzed the glomerularcompartment, 22 the tubulointerstitial, and the vascular. Both manual techniques and image analysis software such as ImageJ, ImagePro-Plus, and QuPath were used. In the glomerular compartment, parameters including cellularity, mesangial expansion, proliferation, and immune deposits were assessed. Tubulointerstitial studies measured fibrosis, tubular atrophy, and immune Infiltrates, while vascular analyses examined intimal thickening and complementactivation. Across compartments, morphometry consistently outperformed conventional visual evaluation, particularly in fibrosis quantification and prediction of renal prognosis. Overall, digital morphometry represents a valuable method for the comprehensive assessment of LN renal biopsies. Its application provides greater precision in characterizing renal damage and offers advantages interms of objectivity, reproducibility, and prognostic value. Standardization of morphometric approaches, together with integration into artificial intelligence-based tools, could optimize diagnostic accuracy and therapeutic strategies in LN.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"53-71"},"PeriodicalIF":1.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel liquid immunocytochemistry with machine learning analysis for bladder cancer detection. 新型液体免疫细胞化学与机器学习分析用于膀胱癌检测。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2026-03-01 Epub Date: 2025-08-15 DOI: 10.1080/01478885.2025.2546655

Ankush U Patel, Samir Atiya, Yi Song, Wenjiang Chu, Anil V Parwani

{"title":"Novel liquid immunocytochemistry with machine learning analysis for bladder cancer detection.","authors":"Ankush U Patel, Samir Atiya, Yi Song, Wenjiang Chu, Anil V Parwani","doi":"10.1080/01478885.2025.2546655","DOIUrl":"10.1080/01478885.2025.2546655","url":null,"abstract":"Bladder cancer diagnosis is challenged by invasive monitoring and workflow inefficiencies impacting diagnostic reliability. This prospective study enrolled 150 patients (January 2020-December 2022) and evaluated a novel liquid-based immunocytochemistry platform, coupled with integrated machine learning, for detecting urothelial carcinoma in voided urine. All 150 cytology slides met the adequacy threshold of ≥2,644 urothelial cells and showed preserved cytomorphology. Eight cases of papillary urothelial neoplasm of low malignant potential (PUNLMP) were set aside a-priori, yielding an analytic cohort of 142 patients (115 urothelial-carcinoma, 27 benign) for performance analysis. hTERT (sensitivity 92.2%, specificity 66.7%), GATA-3 (67.0%, 88.9%), and CK17 (89.6%, 66.7%). In multi-marker analysis, sensitivity reached 100% (95% CI 96.8-100) when any marker was positive, whereas specificity reached 100% (95% CI 87.3-100) when all three markers were positive. The workflow-optimized platform standardizes specimen preparation and multi-marker interpretation, offering a robust foundation for urine-based bladder-cancer diagnostics. Larger, multi-center validation studies are warranted to refine specificity estimates and facilitate laboratory integration. This study demonstrates that addressing fundamental workflow challenges in bladder cancer diagnostics before implementing artificial intelligence creates more effective diagnostic tools. By prioritizing specimen integrity and standardization through a novel liquid immunocytochemistry platform, exceptional diagnostic performance was achieved with 100% sensitivity and specificity under defined marker parameters across various cancer stages. This workflow-first approach to integrating machine learning with advanced biomarker analysis offers a model for developing clinically practical diagnostic innovations that can reduce reliance on invasive monitoring procedures while improving detection accuracy.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"4-12"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An automatic, rapid and accurate method for the annotation of tumor components on whole slide images. 一种自动、快速、准确的肿瘤成分全切片标注方法。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2026-03-01 Epub Date: 2026-01-02 DOI: 10.1080/01478885.2025.2602268

Hong Tang, Xiaodong Wang, Xiaolin Zhang, Xiaojun Wu, Xinyue Tang, Yaqiong Ma, Ying Chen, Guanzhen Yu

{"title":"An automatic, rapid and accurate method for the annotation of tumor components on whole slide images.","authors":"Hong Tang, Xiaodong Wang, Xiaolin Zhang, Xiaojun Wu, Xinyue Tang, Yaqiong Ma, Ying Chen, Guanzhen Yu","doi":"10.1080/01478885.2025.2602268","DOIUrl":"10.1080/01478885.2025.2602268","url":null,"abstract":"Annotated pathology datasets are the cornerstone for developing computational pathology. However, the intrinsic complexity of pathology data often results in a scarcity of large, manually annotated datasets. To address this challenge, a rapid, accurate, and automatic method for annotating tumor cells is essential for advancing the field of computational pathology. Here, we introduce a novel annotation technology. In our approach, Hematoxylin-eosin (H&E) slides were first digitized and preserved. These H&E slides were then faded and re-stained using multiple biological technologies (MBT) to identify specific biomarkers. The re-stained MBT slides were subsequently scanned again to create new digital images. The original and re-stained digital images underwent a registration and segmentation process to ensure that all minute structures in both images aligned perfectly. The staining results from the MBT slides, referred to as2 label maps, were extracted and transferred back onto the H&E slides, and the data were merged. This method allows for precise and efficient annotation of all tumor cells, including those expressing specific biomarkers, as well as the components of the tumor microenvironment. By rapidly generating high-quality, high-volume datasets, this innovative method significantly enhances the ability of AI approaches to analyze and interpret pathology data. Consequently, it supports the development of highly accurate diagnostic, prognostic, and predictive decision-making systems in the field of computational pathology.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"13-25"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145889283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0