Journal of Histotechnology最新文献_第3页

Morphometric study of bioprinted hydrogel deformation during histological preparation. 生物打印水凝胶在组织学制备过程中变形的形态计量学研究。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-09-09 DOI: 10.1080/01478885.2025.2539624

Lucie Essayan, Alexandre Dufour, Emma Petiot, Christophe Marquette

{"title":"Morphometric study of bioprinted hydrogel deformation during histological preparation.","authors":"Lucie Essayan, Alexandre Dufour, Emma Petiot, Christophe Marquette","doi":"10.1080/01478885.2025.2539624","DOIUrl":"https://doi.org/10.1080/01478885.2025.2539624","url":null,"abstract":"Histological preparation via paraffin embedding is the gold standard method for evaluating tissue structure and composition, whether it is originated from biopsy or engineered in vitro. Quite often, deformation and shrinkage occur during the histological preparation, which are difficult to predict and qualify. The present study investigates the morphometric changes in bioprinted hydrogels composed of alginate and gelatine, common tissue engineering materials, focusing on three morphologies: full slabs, porous slabs, and porous cubes. These structures underwent key histological steps, including fixation, processing (dehydration, clearing, and infiltration with melted paraffin), embedding, and slicing, to evaluate their shrinkage behavior. Shrinking factors were systematically measured, showing that processing had the most significant effect (34-40% shrinking), followed by fixation (20-28% shrinking). Porous structures exhibited greater shrinkage compared to full slabs due to their internal geometry. Additionally, anisotropic behavior was observed in porous cubes, with different shrinking factors in the XY plane (horizontal) and Z direction (vertical), leading to an overall volumetric shrinking factor of 81.3%. The results demonstrated the critical influence of hydrogel structure on deformation and emphasized the need for tailored histological protocols to maintain structural fidelity. While this study focused on hydrogels alone, future work will incorporate cellularized bioengineered tissues to evaluate the impact of cell-mediated remodeling and extracellular matrix deposition on histological outcomes. This research offers a framework for optimizing histological preparation in bioengineered tissues, enabling more accurate assessment of their structure and function for regenerative medicine applications.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":1.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preservation of tophi in the modified Gomori methenamine silver procedure for urates. 用改良的戈莫里甲基苯丙胺银法制备尿酸。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-09-03 DOI: 10.1080/01478885.2025.2550125

Lauren Van Aken, Andrew Bielli, Joseph A Esposito

引用次数: 0

Corticoclaustral connections in the cat. 猫的皮质闭侧连接。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-09-01 Epub Date: 2025-03-14 DOI: 10.1080/01478885.2025.2476835

B Landzhov, D Hinova-Palova, K Fakih, L Edelstein, L Gaydarski, A Alexandrov, V Kirkov, A Paloff, E Radeva

{"title":"Corticoclaustral connections in the cat.","authors":"B Landzhov, D Hinova-Palova, K Fakih, L Edelstein, L Gaydarski, A Alexandrov, V Kirkov, A Paloff, E Radeva","doi":"10.1080/01478885.2025.2476835","DOIUrl":"10.1080/01478885.2025.2476835","url":null,"abstract":"The claustrum is a sheet-like layer of gray matter situated between the external and extreme capsules of the mammalian brain. This structure was first described by the French physician and anatomist Vicq d'Azyr in 1786. The claustrum's phylogeny, ontogeny and functional characteristics have long been the subject of debate and considerable investigative efforts. However, despite such efforts (or perhaps as a result thereof), significant disparities and discrepancies remain, most notably in the context of the claustrum's afferent and efferent connections. For the purpose of this study, we sought to focus our efforts on fronto-claustral and occipito-claustral connections. Twelve healthy, adult male cats, each weighing ~ 3.5 kg, were studied, seven of which underwent electrolytic lesions of the frontal cortex (A3, A4, and a portion of A6), and five of the occipital cortex (A17, A18, A21). From three to six days after lesioning, subjects were euthanized in accordance with ethical norms. After the brains were removed and blocked, samples of the claustrum were taken and prepared for electron microscopy. Three to six days after lesions of the frontal cortex, we observed an abundance of degenerative boutons in the dorsal claustrum. The vast majority of boutons exhibited the characteristic signs of dark degeneration, whereas only 10% appeared to have undergone light degeneration. Similar results were seen in the dorsal claustrum over the same period of time following lesions of the visual cortex. These results suggest that the dorsal claustrum receives at least two types of connections - separately and concurrently - from the frontal and occipital cortices.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"146-156"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utilizing image analysis by optical density to evaluate changes in hematoxylin and eosin staining quality after reagent overuse. 利用光密度图像分析评价试剂过度使用后苏木精和伊红染色质量的变化。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-09-01 Epub Date: 2025-06-23 DOI: 10.1080/01478885.2025.2517906

Hannah M Benton, Mark Butters, Miles Brous, Brad Bolon, Karen Copeland, Jessica S Fortin, Elizabeth Chlipala

{"title":"Utilizing image analysis by optical density to evaluate changes in hematoxylin and eosin staining quality after reagent overuse.","authors":"Hannah M Benton, Mark Butters, Miles Brous, Brad Bolon, Karen Copeland, Jessica S Fortin, Elizabeth Chlipala","doi":"10.1080/01478885.2025.2517906","DOIUrl":"10.1080/01478885.2025.2517906","url":null,"abstract":"Due to the prevalence of hematoxylin and eosin (H&E) staining in routine histological preparations, understanding the factors that impact stain color characteristics is vital to attain consistently high-quality stains. In the last decade, increased use of digital pathology and image analysis (specifically by optical density [OD] measures) has provided new ways of assessing staining precision. This paper combines data from two studies that tracked H&E staining quality in both nuclear and cytoplasmic components of 12 tissues (11 human and 1 porcine) by OD after overuse of H&E staining reagents from 5 vendors. Both studies showed a decrease in eosin stain intensity by OD and visual inspection (by a histologist) with reagent overuse. This trend varied in degree by tissue type and reagent vendor. Nonetheless, staining quality for H&E staining from all vendors and for all organs remained acceptable (but not always optimal) for microscopic evaluation by the College of American Pathologists (CAP) and National Society for Histotechnology (NSH) staining criteria when stained sections were reviewed by a board-certified veterinary pathologist.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"123-134"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing tissue adherence on glass slides using polyurethane glue: a new slide preparation method. 利用聚氨酯胶优化组织粘附在玻片上：一种新的玻片制备方法。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-09-01 Epub Date: 2024-12-17 DOI: 10.1080/01478885.2024.2440679

Jeffery T Williams, Tracy A Goodpaster, Miki Haraguchi

引用次数: 0

Preserving brain health by minimizing microplastic output from resin histology. 通过减少树脂组织的微塑性输出来保护大脑健康。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-09-01 Epub Date: 2025-01-07 DOI: 10.1080/01478885.2024.2449279

Tia Tedford

引用次数: 0

Hyperglycemia and insulin treatment promote the proliferation of retinal pigment epithelium cells in early diabetes: an in vitro and in vivo study. 高血糖和胰岛素治疗促进早期糖尿病视网膜色素上皮细胞的增殖：一项体外和体内研究。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-09-01 Epub Date: 2025-05-16 DOI: 10.1080/01478885.2025.2503520

Qiurong Lin, Fan Yang, Xiaofeng Zhu, Haidong Zou, Wei Xu

{"title":"Hyperglycemia and insulin treatment promote the proliferation of retinal pigment epithelium cells in early diabetes: an in vitro and in vivo study.","authors":"Qiurong Lin, Fan Yang, Xiaofeng Zhu, Haidong Zou, Wei Xu","doi":"10.1080/01478885.2025.2503520","DOIUrl":"10.1080/01478885.2025.2503520","url":null,"abstract":"The retinal pigment epithelium (RPE) is associated with the emergence and development of diabetic retinopathy. Interestingly, a previous clinical study observed that the atrophy of RPE cells surrounding the optic disc in type 1 diabetic children were significantly less pronounced compared to normal children, contradicting current reports. In order to explore the molecular mechanisms behind this contradictory phenomenon, we conducted a series of experiments and reached the following results. First, RPE cells proliferation increased in a glucose concentration-dependent manner in vitro, accompanied by elevated Brachyury and CTGF protein expression, but decreased overall cell viability. Secondly, in vitro experiments and diabetes mouse models confirmed that insulin promoted RPE cell proliferation in high glucose concentrations by activating ERK1/2 phosphorylation. Furthermore, insulin down-regulated the expression of Brachyury and CTGF proteins, possibly reducing high-glucose‒induced damage to RPE cells. In conclusion, the effect of insulin treatment on the proliferation of RPE cells was significantly more significant than that of hyperglycemia, which may be related to the activation of Erk1/2 or reduction of RPE cell damage by inhibiting the occurrence of EMT.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"135-145"},"PeriodicalIF":1.8,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paraffin sectioning in the modern laboratory and the problem of static electricity: a review. 现代实验室石蜡切片与静电问题综述。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-09-01 Epub Date: 2025-08-14 DOI: 10.1080/01478885.2025.2544011

Ronald Sluys, Sytske de Waart

引用次数: 0

Creating synergy between established histology methods and newer spatial analysis modalities using fresh frozen tissues. 在已建立的组织学方法和使用新鲜冷冻组织的较新的空间分析模式之间创造协同作用。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-07-23 DOI: 10.1080/01478885.2025.2530258

Emily R Sekera, Anoop Murthy Kavirayani, Pam Johnson, Heather Sheppard, Roman Kiselev, John J Bowling

{"title":"Creating synergy between established histology methods and newer spatial analysis modalities using fresh frozen tissues.","authors":"Emily R Sekera, Anoop Murthy Kavirayani, Pam Johnson, Heather Sheppard, Roman Kiselev, John J Bowling","doi":"10.1080/01478885.2025.2530258","DOIUrl":"https://doi.org/10.1080/01478885.2025.2530258","url":null,"abstract":"Formalin-fixed paraffin embedded tissues are a mainstay in contemporary histology and pathology practice and represent the most readily available and widely used sample format that has guided early studies and set standards for a new field of spatial biology. However, fixed tissues are not always suitable for all molecular applications and detection of certain molecular targets can be suppressed by these practices. There is a greater diversity of analysis techniques that are commercially available to researchers today, and pathologists and core services are now engaging with groups that prefer fresh-frozen samples more than ever before. Some of these analysis techniques can be characterized as 'label-free' and access unique classes of molecular targets in situ. Our team has developed tools and procedures to facilitate a synergy between the realm of label-free spatial biology and the histology laboratory for biomedical discovery programs. Herein, we discuss the development of 3D printed cassette holders for use in a variety of commercially available cryostats. This apparatus was tested using fresh frozen tissue slices embedded in hydroxypropyl methylcellulose polyvinylpyrrolidone for subsequent analyses. Based on our tests, we propose this protocol as an option to better preserve sample history during the lifetime of a specimen and curtail the decomposition of any lower abundance endogenous chemical moieties for spatial 'omics' analysis using mass spectrometry.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":0.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic yield of stains for infectious organisms in esophageal or gastroesophageal junction biopsies with esophagitis. 食管炎食管或胃食管交界处活检中感染性生物体染色的诊断率。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-06-01 Epub Date: 2024-10-14 DOI: 10.1080/01478885.2024.2415154

Aaron R Huber, Christa L Whitney-Miller

引用次数: 0