Journal of Histotechnology最新文献_第3页

Using machine learning for chemical-free histological tissue staining 利用机器学习进行无化学物质组织染色

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-04-22 DOI: 10.1080/01478885.2024.2338585

Julie A. Renner, Patrick C. Riley

引用次数: 0

Molecular surgical pathology, 2nd edition 分子外科病理学，第 2 版

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-04-16 DOI: 10.1080/01478885.2024.2343180

Stephen K. Lau

引用次数: 0

A rapid and simple procedure for the whole-mount bone staining of small fish 小鱼整块骨染色的快速简便程序

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-04-12 DOI: 10.1080/01478885.2024.2339452

Narawadee Yeesan, Chanyut Sudtongkong, Supparat Kong-Oh, Kitipong Angsujinda, Jes Kettratad, Pisit Poolprasert, Atsuo Iida, Piyakorn Boonyoung, Korakot Nganvongpanit, Anan Kenthao, Gen Kaneko, Sinlapachai Senarat

引用次数: 0

Novel processing and staining methodology of bottlenose dolphin (Tursiops truncatus) teeth for age determination. 用于年龄测定的瓶鼻海豚（Tursiops truncatus）牙齿新处理和染色方法。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-03-11 DOI: 10.1080/01478885.2024.2327155

Christa E Barrett, Ian Evans, Timothy Morgan, Alicia K Olivier

{"title":"Novel processing and staining methodology of bottlenose dolphin (Tursiops truncatus) teeth for age determination.","authors":"Christa E Barrett, Ian Evans, Timothy Morgan, Alicia K Olivier","doi":"10.1080/01478885.2024.2327155","DOIUrl":"https://doi.org/10.1080/01478885.2024.2327155","url":null,"abstract":"Age determination of bottlenose dolphins (Tursiops truncatus) is a critical tool in understanding both individual and population health. There are many methods of aging bottlenose dolphins including analysis of teeth, pectoral flipper radiographs, and epigenetics. The most common and oldest method for aging toothed cetaceans is the counting of growth layer groups (GLGs) in the teeth. Current techniques have technical and repeatability challenges. Therefore, a processing technique that results in better resolution of GLGs is needed. This study compares different decalcifications and different histochemical staining techniques. Decalcification was done using 10% EDTA, Kristensen's decalcification, and Rapid Decalcification Solution (RDO). Following decalcification and routine processing, GLGs were assessed using Hematoxylin and Eosin (H&E), hematoxylin, Giemsa, Wright-Giemsa, Toluidine Blue (T-Blue), Masson's Trichrome, and Congo Red staining techniques. Decalcification with Kristensen's and staining with Masson's Trichrome and Congo Red were determined to best highlight GLGs. This processing and staining was then applied to a sample population of 102 bottlenose dolphins that were evaluated independently and blindly by two observers. Of the 102 dolphin samples, 13 (12.7%) were unable to age due to no clear distinction or distortion between GLGs.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The presence of H. pylori in laparoscopic sleeve gastrectomy specimens is associated with increased mucosal thickness, presence of secondary follicles, increased chronic inflammation, and intestinal metaplasia. 腹腔镜袖状胃切除术标本中幽门螺杆菌的存在与粘膜厚度增加、次级卵泡存在、慢性炎症增加和肠化生有关。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-03-01 Epub Date: 2023-10-12 DOI: 10.1080/01478885.2023.2265601

Danielle Grant, Chelsea Peeler, JoAnna Rudasill, Cynthia Lazar, Amanda Bodkin, Sheila L Criswell

{"title":"The presence of H. pylori in laparoscopic sleeve gastrectomy specimens is associated with increased mucosal thickness, presence of secondary follicles, increased chronic inflammation, and intestinal metaplasia.","authors":"Danielle Grant, Chelsea Peeler, JoAnna Rudasill, Cynthia Lazar, Amanda Bodkin, Sheila L Criswell","doi":"10.1080/01478885.2023.2265601","DOIUrl":"10.1080/01478885.2023.2265601","url":null,"abstract":"Helicobacter pylori is putatively present in over half of the global human population and is recognized as a carcinogenic agent that increases the likelihood of infected patients developing gastric adenocarcinoma or gastric lymphoma. Although there are several means for testing for H. pylori, the gold standard remains the invasive histologic evaluation. The current most popular form of bariatric surgery is the laparoscopic sleeve gastrectomy (LSG) and is the only bariatric surgery which supplies a specimen for histologic evaluation. While non-invasive testing is effective in diagnosing and monitoring H. pylori infection, histological examination of biopsies and resections is the only way to grade chronic inflammation and evaluate specimens for additional pathologies such as intestinal metaplasia. The investigators evaluated 203 sequential LSG specimens collected from a major metropolitan hospital over the period of one year. Specimens were processed to paraffin, stained with hematoxylin and eosin, alcian blue, and immunohistochemistry to determine the presence of H. pylori, chronic inflammation, presence of secondary lymphoid follicles in the mucosa, mucosal thickness, and presence of intestinal metaplasia. Statistical analyses demonstrated a significant positive correlation among all factors examined. The overall positivity rate of H. pylori in LSG specimens was 18.2% but ranged from 6.9-23.8% depending on whether the treating clinician performed routine pre-surgical endoscopy. The presence of H. pylori was associated with a higher average chronic inflammation grade, intestinal metaplasia, thicker mucosa, and presence of lymphoid follicles with germinal centers in the mucosa.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41203065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histomorphogenesis of sublingual salivary gland of Indian sheep. 印度绵羊舌下唾液腺的组织形态发生。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-03-01 Epub Date: 2023-10-24 DOI: 10.1080/01478885.2023.2266799

Amandeep Singh, Simran Kaur, Opinder Singh

{"title":"Histomorphogenesis of sublingual salivary gland of Indian sheep.","authors":"Amandeep Singh, Simran Kaur, Opinder Singh","doi":"10.1080/01478885.2023.2266799","DOIUrl":"10.1080/01478885.2023.2266799","url":null,"abstract":"The primordial anlage of sublingual gland was first noticed as a solid epithelial bud from oral epithelium at the 24th day of foetal development. The terminal buds were arranged in the form of clusters with undifferentiated epithelial cells and terminated in a bulb-like structure in the 30-day-old sheep foetus. On the 37th day, lumenization and branching of the main cord was noticed. The primary septa were observed from the 55th day onwards which resulted in the formation of lobulation on the 60th day. The capsule formation was initiated by aggregation of mesenchymal tissue on the 63rd day. On the 100th day, terminal tubules differentiated to form secretory end pieces. Tubular portions formed intercalated and striated ducts. Predominantly mucous type of acinar cells was seen from the 110th day onwards with myoepithelial cells. The number of lobules increased with increase in parenchyma from 130th day onwards. Micrometrical studies revealed that the mean diameter of acini, intercalated, striated and large ducts was increased with advancement of age and significant differences were observed between groups. Localization of acidic and neutral mucopolysaccharides were observed in mucous and goblet cells. Fine lipid droplets were observed in intralobular and interlobular connective tissue however, phospholipids were observed in cell membrane of acini and ducts. The current investigation provides microstructural standards for the organogenesis of the sublingual gland of miniature sheep and can lay the foundation for further studies in the morphological investigation of salivary gland development.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49690848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of electron microscopy in virus diagnosis and advanced therapeutic approaches. 电子显微镜在病毒诊断和先进治疗方法中的应用。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-03-01 Epub Date: 2024-01-19 DOI: 10.1080/01478885.2024.2306076

Fengli Guo

引用次数: 0

Invisible for a few but essential for many: the role of Histotechnologists in the establishment of digital pathology. 对少数人来说是看不见的，但对许多人来说是必不可少的：组织技术专家在建立数字病理学中的作用。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-03-01 Epub Date: 2023-10-23 DOI: 10.1080/01478885.2023.2268297

Gisela Magalhães, Rita Calisto, Catarina Freire, Regina Silva, Diana Montezuma, Sule Canberk, Fernando Schmitt

{"title":"Invisible for a few but essential for many: the role of Histotechnologists in the establishment of digital pathology.","authors":"Gisela Magalhães, Rita Calisto, Catarina Freire, Regina Silva, Diana Montezuma, Sule Canberk, Fernando Schmitt","doi":"10.1080/01478885.2023.2268297","DOIUrl":"10.1080/01478885.2023.2268297","url":null,"abstract":"Digital pathology (DP) is indisputably the future for histopathology laboratories. The process of digital implementation requires deep workflow reorganisation which involves an interdisciplinary team. This transformation may have the greatest impact on the Histotechnologist (HTL) profession. Our review of the literature has clearly revealed that the role of HTLs in the establishment of DP is being unnoticed and guidance is limited. This article aims to bring HTLs from behind-the-scenes into the spotlight. Our objective is to provide them guidance and practical recommendations to successfully contribute to the implementation of a new digital workflow. Furthermore, it also intends to contribute for improvement of study programs, ensuring the role of HTL in DP is addressed as part of graduate and post-graduate education. In our review, we report on the differences encountered between workflow schemes and the limitations observed in this process. The authors propose a digital workflow to achieve its limitless potential, focusing on the HTL's role. This article explores the novel responsibilities of HTLs during specimen gross dissection, embedding, microtomy, staining, digital scanning, and whole slide image quality control. Furthermore, we highlight the benefits and challenges that DP implementation might bring the HTLs career. HTLs have an important role in the digital workflow: the responsibility of achieving the perfect glass slide.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49690849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An ultrastructural report of human articular cartilage resident cells in correlation with their phenotypic characteristics. 人关节软骨驻留细胞及其表型特征的超微结构研究报告。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-03-01 Epub Date: 2023-11-15 DOI: 10.1080/01478885.2023.2278118

Deepak Vinod Francis, Aishwarya Jayakumaran Rajeswari, John Bino Stephen, Ganesh Parasuraman, Jeya Lisha J, Abel Livingston, Sandya Rani, Alfred Job Daniel, Solomon Sathishkumar, Elizabeth Vinod

{"title":"An ultrastructural report of human articular cartilage resident cells in correlation with their phenotypic characteristics.","authors":"Deepak Vinod Francis, Aishwarya Jayakumaran Rajeswari, John Bino Stephen, Ganesh Parasuraman, Jeya Lisha J, Abel Livingston, Sandya Rani, Alfred Job Daniel, Solomon Sathishkumar, Elizabeth Vinod","doi":"10.1080/01478885.2023.2278118","DOIUrl":"10.1080/01478885.2023.2278118","url":null,"abstract":"The recent discovery of progenitors based on their differential fibronectin-adhesion (FAA-CPs) and migratory-based (MCPs) assay has evoked interest due to their superiority in terms of their efficient chondrogenesis and reduced hypertrophic propensity. This study aims to isolate and enrich three articular cartilage subsets, chondrocytes, FAA-CPs, and MCPs, and compare their undifferentiated and chondrogenic differentiated status, using in-vitro phenotypical characterization in correlation with ultrastructural analysis using Transmission Electron Microscopy (TEM). Following informed consent, cartilage shavings were procured from a non-diseased human ankle joint and cultured to obtain the three subsets. Chondrocytes exhibited higher CD106 and lower CD49b and CD146 levels. Following chondrogenic differentiation, corroborative results were seen, with the MCP group showing the highest GAG/DNA ratio levels and uptake of extracellular matrix stain as compared to the FAA-CP group. TEM analysis of the chondrocytes revealed the presence of more autolytic cells with disintegrated cytoplasm and plasma membrane. The differentiated FAA-CPs and MCPs displayed higher collagen and rough endoplasmic reticulum. The results presented in this study provide novel information on the ultrastructural characteristics of cartilage resident cells, with the chondrocyte group displaying features of terminal differentiation. Both progenitor subtypes showed superiority in varied contexts, with greater collagen fibrils and greater GAG content in MCPs. The display of preferential and differentiation traits sheds insight on the necessity to enrich progenitors and coculturing them with the general pool of constituent cells to combine their advantages and reduce their drawbacks to achieve a regenerative tissue displaying genuine hyaline-like repair while limiting their terminal differentiation.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107591454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathology: historical and contemporary aspects 病理学：历史与当代

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-01-15 DOI: 10.1080/01478885.2024.2303911

Stephen K. Lau

引用次数: 0