Journal of Histotechnology最新文献_第3页

Comparative analysis of in vivo bio-integration of three hyaluronic acid-based fillers for 26 weeks: a histological study. 三种透明质酸类填充剂 26 周体内生物整合比较分析：组织学研究。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-01 Epub Date: 2024-07-18 DOI: 10.1080/01478885.2024.2369967

Baoji Song, Qiqi Chen

引用次数: 0

Novel processing and staining methodology of bottlenose dolphin (Tursiops truncatus) teeth for age determination. 用于年龄测定的瓶鼻海豚（Tursiops truncatus）牙齿新处理和染色方法。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-01 Epub Date: 2024-03-11 DOI: 10.1080/01478885.2024.2327155

Christa E Barrett, Ian Evans, Timothy Morgan, Alicia K Olivier

{"title":"Novel processing and staining methodology of bottlenose dolphin (Tursiops truncatus) teeth for age determination.","authors":"Christa E Barrett, Ian Evans, Timothy Morgan, Alicia K Olivier","doi":"10.1080/01478885.2024.2327155","DOIUrl":"10.1080/01478885.2024.2327155","url":null,"abstract":"Age determination of bottlenose dolphins (Tursiops truncatus) is a critical tool in understanding both individual and population health. There are many methods of aging bottlenose dolphins including analysis of teeth, pectoral flipper radiographs, and epigenetics. The most common and oldest method for aging toothed cetaceans is the counting of growth layer groups (GLGs) in the teeth. Current techniques have technical and repeatability challenges. Therefore, a processing technique that results in better resolution of GLGs is needed. This study compares different decalcifications and different histochemical staining techniques. Decalcification was done using 10% EDTA, Kristensen's decalcification, and Rapid Decalcification Solution (RDO). Following decalcification and routine processing, GLGs were assessed using Hematoxylin and Eosin (H&E), hematoxylin, Giemsa, Wright-Giemsa, Toluidine Blue (T-Blue), Masson's Trichrome, and Congo Red staining techniques. Decalcification with Kristensen's and staining with Masson's Trichrome and Congo Red were determined to best highlight GLGs. This processing and staining was then applied to a sample population of 102 bottlenose dolphins that were evaluated independently and blindly by two observers. Of the 102 dolphin samples, 13 (12.7%) were unable to age due to no clear distinction or distortion between GLGs.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"146-153"},"PeriodicalIF":0.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Addressing the histotechnologist shortage through improved classification and recognition. 通过改进分类和识别，解决组织技术人员短缺问题。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI: 10.1080/01478885.2024.2424049

Henley Marcelus, Daniel Packert

引用次数: 0

A study of PGP 9.5 immunohistochemical labeling on formalin-fixed paraffin embedded tissues for epidermal nerve fiber density testing 对用于表皮神经纤维密度测试的福尔马林固定石蜡包埋组织进行 PGP 9.5 免疫组化标记的研究

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-09-17 DOI: 10.1080/01478885.2024.2405418

Joseph A. Esposito, Camryn J. Vader, Joel R. Israel, Steve A. McClain

引用次数: 0

Survey of NAACLS accredited histotechnology programs in the United States. 美国经 NAACLS 认证的组织技术课程调查。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-03-18 DOI: 10.1080/01478885.2024.2327095

Carol Bain, Debra Wood, Sheila Criswell

{"title":"Survey of NAACLS accredited histotechnology programs in the United States.","authors":"Carol Bain, Debra Wood, Sheila Criswell","doi":"10.1080/01478885.2024.2327095","DOIUrl":"10.1080/01478885.2024.2327095","url":null,"abstract":"Histotechnology educational programs are accredited by the National Accrediting Agency for Clinical Laboratory Sciences (NAACLS) and currently number fewer than 50 in the United States which contributes to the shortages of laboratory personnel. A survey tool designed with REDCap software was distributed to all programs identified on the NAACLS website and consisted of three parts: a) program information, b) facility information, and c) challenges. Programs are located primarily in large urban centers where populations are most concentrated. The median class size was 6 which may explain the excellent student outcomes to include 96% graduation rates and 90.7% board of registry examination pass rates. Overall, programs had ample equipment, funding, and administrative support. Costs to attend the programs were relatively low (<$3,000 per semester) for over half of the programs. However, due to the small number of accredited education programs across the US, potential students do not often have access to an institution in their area. The programs indicated that the most common challenge was recruitment of adequate high-quality candidates which may explain, in part, the persistent shortage of personnel in the histology laboratory.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"109-116"},"PeriodicalIF":0.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140143574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of light and electron microscopic morphometric parameters of glomerular capillaries with serum creatinine and proteinuria. 肾小球毛细血管的光镜和电子显微镜形态参数与血清肌酐和蛋白尿的相关性。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-03-11 DOI: 10.1080/01478885.2024.2326274

Dibyajyoti Boruah, A W Kashif, Barun Kumar Chakrabarty, Sarika Harikrishnan, Arijit Sen

{"title":"Correlation of light and electron microscopic morphometric parameters of glomerular capillaries with serum creatinine and proteinuria.","authors":"Dibyajyoti Boruah, A W Kashif, Barun Kumar Chakrabarty, Sarika Harikrishnan, Arijit Sen","doi":"10.1080/01478885.2024.2326274","DOIUrl":"10.1080/01478885.2024.2326274","url":null,"abstract":"Waste products in the bloodstream are filtered by the glomerular capillaries in the kidneys and excreted into the urine. When making a differential diagnosis of kidney diseases, structural assessment of glomeruli using histological, ultrastructural, and immunological studies is crucial. This study assessed the microscopic and ultrastructural morphometric parameters of glomerular capillaries and examined their correlation with serum creatinine and proteinuria. A total of 60 kidney biopsy cases received by the transmission electron microscope (TEM) laboratory for diagnosis were included in the study. Toluidine blue stained 300 nm thick sections of TEM tissue blocks were scanned for glomerular morphometry by a whole slide imaging system, and the estimation of Bowman's capsule (BC) area, glomerular capillary lumen diameter (GCLD), glomerular capillary density (GCD), glomerular capillary surface area density (GCSA), and percentage of glomerular capillary lumen space (%GCLS) was performed with QuPath software. TEM images of 70 nm thick sections were used for the evaluation of endothelial fenestration diameter (EFD), glomerular basement membrane (GBM) thickness, and podocyte foot process (PFP) effacement. Proteinuria and serum creatinine showed positive correlations with GBM thickness and PFP effacement. Negative correlations of serum creatinine were observed with EFD, %GCLS, and GCSA. Hence, glomerular filtration is greatly affected by the total area of the glomerular capillary surface and structural changes of GBM. Reduction of glomerulus filtration due to foot process effacement and thickening of GBM results in damage to the filtration barrier leading to the leakage of plasma protein into urine.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"97-108"},"PeriodicalIF":0.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of degenerated synaptic boutons in the dorsal claustrum of the cat after electrolytic lesions of the intralaminar thalamic nuclei. 鉴定丘脑内核电解损伤后猫背侧丘脑中退化的突触突触。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-04-02 DOI: 10.1080/01478885.2024.2335827

Dimka Hinova-Palova, Boycho Landzhov, Lawrence Edelstein, Khodor Fakih, Alexandar Alexandrov, Teodora Kiriakova, Elka Radeva, Lyubomir Gaydarski, Frank Denaro, Adrian Paloff

{"title":"Identification of degenerated synaptic boutons in the dorsal claustrum of the cat after electrolytic lesions of the intralaminar thalamic nuclei.","authors":"Dimka Hinova-Palova, Boycho Landzhov, Lawrence Edelstein, Khodor Fakih, Alexandar Alexandrov, Teodora Kiriakova, Elka Radeva, Lyubomir Gaydarski, Frank Denaro, Adrian Paloff","doi":"10.1080/01478885.2024.2335827","DOIUrl":"10.1080/01478885.2024.2335827","url":null,"abstract":"The aim of this study is to investigate whether the dorsal claustrum receives afferent input from the intralaminar thalamic nuclei - centromedian nucleus, central lateral nucleus and paracentral nucleus. The intralaminar thalamic nuclei of eight cats were electrolytically lesioned. We obtained samples from the dorsal claustrum for electron microscopic analysis from the second to the seventh post-procedural day. Two types of degenerated synaptic boutons were observed: electron-dense which formed the majority of boutons, and electron-lucent comprising the remaining samples. Between the second and seventh post-procedural day, we observed a steady increase in the number of electron-dense boutons which were diffusely distributed throughout the dorsal claustrum. Electron-dense degenerated boutons formed asymmetrical contacts with dendritic spines as well as with small and medium-sized dendrites. In contrast, electron-lucent degenerated boutons were observed in earlier post-procedural periods and formed symmetrical axodendritic contacts.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"117-125"},"PeriodicalIF":0.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What will it take for histologists to be recognized under CLIA? 组织学家如何才能获得 CLIA 的认可？

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-08-30 DOI: 10.1080/01478885.2024.2391636

Elizabeth Chlipala, Tim Morken, Clare Thornton, Luis Chiriboga

引用次数: 0

Tissue clearing and three-dimensional imaging of intact tissues: a review on FACT protocol. 完整组织的组织清除和三维成像：FACT 方案综述。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-05-16 DOI: 10.1080/01478885.2024.2352695

Zohreh Farrar, Alireza Afshar, Afshin Zare, Nadiar M Mussin, Asset A Kaliyev, Kulyash R Zhilisbayeva, Mahdi Mahdipour, Amin Tamadon

{"title":"Tissue clearing and three-dimensional imaging of intact tissues: a review on FACT protocol.","authors":"Zohreh Farrar, Alireza Afshar, Afshin Zare, Nadiar M Mussin, Asset A Kaliyev, Kulyash R Zhilisbayeva, Mahdi Mahdipour, Amin Tamadon","doi":"10.1080/01478885.2024.2352695","DOIUrl":"10.1080/01478885.2024.2352695","url":null,"abstract":"FACT is a developed technique for clearing tissues that does not use acrylamide. Since the removal of lipids is crucial for transparency and efficient antibody staining throughout the tissue, especially for microscopy and imaging, it is a harmful process that can cause the loss of important biological molecules such as proteins. The FACT technique overcomes this by chemically bonding the membrane and intracellular proteins with the extracellular matrix, creating a massive 3D hydrogel matrix and providing structural support to fortify the tissue during processing. Compared to other acrylamide-based techniques, the FACT technique requires less labor and harmful chemicals and is therefore considered a more suitable option. In this study, we describe the complete FACT protocol for antibody staining and imaging of whole-cleared tissues while preserving structure and improving image quality. The protocol includes tissue perfusion, fixation, clearing, antibody staining, refractive index matching (RIM) (), microscopy, and imaging. The timing for each step varies depending on the size, weight, and type of tissue, as well as the type of immunostaining. We provide an example of the FACT protocol using mouse brain tissue, which demonstrates its suitability for molecular interrogation analysis of large tissues. The FACT technique has been successfully performed on different types of tissues, making it a favorable choice for a variety of applications.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"126-142"},"PeriodicalIF":0.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140944901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increase of KLK7, cytokeratin 5/6, and elafin expression in head and neck squamous cell carcinoma compared with lung squamous cell carcinoma. 与肺鳞癌相比，头颈部鳞癌中 KLK7、细胞角蛋白 5/6 和 elafin 的表达增加。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-06-01 Epub Date: 2024-01-08 DOI: 10.1080/01478885.2023.2301123

Angelin Shanmugam, JoAnna Rudasill, Sheila Criswell

引用次数: 0