Journal of Histotechnology最新文献_第3页

Identification of degenerated synaptic boutons in the dorsal claustrum of the cat after electrolytic lesions of the intralaminar thalamic nuclei. 鉴定丘脑内核电解损伤后猫背侧丘脑中退化的突触突触。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-04-02 DOI: 10.1080/01478885.2024.2335827

Dimka Hinova-Palova, Boycho Landzhov, Lawrence Edelstein, Khodor Fakih, Alexandar Alexandrov, Teodora Kiriakova, Elka Radeva, Lyubomir Gaydarski, Frank Denaro, Adrian Paloff

{"title":"Identification of degenerated synaptic boutons in the dorsal claustrum of the cat after electrolytic lesions of the intralaminar thalamic nuclei.","authors":"Dimka Hinova-Palova, Boycho Landzhov, Lawrence Edelstein, Khodor Fakih, Alexandar Alexandrov, Teodora Kiriakova, Elka Radeva, Lyubomir Gaydarski, Frank Denaro, Adrian Paloff","doi":"10.1080/01478885.2024.2335827","DOIUrl":"10.1080/01478885.2024.2335827","url":null,"abstract":"The aim of this study is to investigate whether the dorsal claustrum receives afferent input from the intralaminar thalamic nuclei - centromedian nucleus, central lateral nucleus and paracentral nucleus. The intralaminar thalamic nuclei of eight cats were electrolytically lesioned. We obtained samples from the dorsal claustrum for electron microscopic analysis from the second to the seventh post-procedural day. Two types of degenerated synaptic boutons were observed: electron-dense which formed the majority of boutons, and electron-lucent comprising the remaining samples. Between the second and seventh post-procedural day, we observed a steady increase in the number of electron-dense boutons which were diffusely distributed throughout the dorsal claustrum. Electron-dense degenerated boutons formed asymmetrical contacts with dendritic spines as well as with small and medium-sized dendrites. In contrast, electron-lucent degenerated boutons were observed in earlier post-procedural periods and formed symmetrical axodendritic contacts.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"117-125"},"PeriodicalIF":0.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What will it take for histologists to be recognized under CLIA? 组织学家如何才能获得 CLIA 的认可？

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-08-30 DOI: 10.1080/01478885.2024.2391636

Elizabeth Chlipala, Tim Morken, Clare Thornton, Luis Chiriboga

引用次数: 0

Tissue clearing and three-dimensional imaging of intact tissues: a review on FACT protocol. 完整组织的组织清除和三维成像：FACT 方案综述。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-05-16 DOI: 10.1080/01478885.2024.2352695

Zohreh Farrar, Alireza Afshar, Afshin Zare, Nadiar M Mussin, Asset A Kaliyev, Kulyash R Zhilisbayeva, Mahdi Mahdipour, Amin Tamadon

{"title":"Tissue clearing and three-dimensional imaging of intact tissues: a review on FACT protocol.","authors":"Zohreh Farrar, Alireza Afshar, Afshin Zare, Nadiar M Mussin, Asset A Kaliyev, Kulyash R Zhilisbayeva, Mahdi Mahdipour, Amin Tamadon","doi":"10.1080/01478885.2024.2352695","DOIUrl":"10.1080/01478885.2024.2352695","url":null,"abstract":"FACT is a developed technique for clearing tissues that does not use acrylamide. Since the removal of lipids is crucial for transparency and efficient antibody staining throughout the tissue, especially for microscopy and imaging, it is a harmful process that can cause the loss of important biological molecules such as proteins. The FACT technique overcomes this by chemically bonding the membrane and intracellular proteins with the extracellular matrix, creating a massive 3D hydrogel matrix and providing structural support to fortify the tissue during processing. Compared to other acrylamide-based techniques, the FACT technique requires less labor and harmful chemicals and is therefore considered a more suitable option. In this study, we describe the complete FACT protocol for antibody staining and imaging of whole-cleared tissues while preserving structure and improving image quality. The protocol includes tissue perfusion, fixation, clearing, antibody staining, refractive index matching (RIM) (), microscopy, and imaging. The timing for each step varies depending on the size, weight, and type of tissue, as well as the type of immunostaining. We provide an example of the FACT protocol using mouse brain tissue, which demonstrates its suitability for molecular interrogation analysis of large tissues. The FACT technique has been successfully performed on different types of tissues, making it a favorable choice for a variety of applications.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"126-142"},"PeriodicalIF":0.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140944901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increase of KLK7, cytokeratin 5/6, and elafin expression in head and neck squamous cell carcinoma compared with lung squamous cell carcinoma. 与肺鳞癌相比，头颈部鳞癌中 KLK7、细胞角蛋白 5/6 和 elafin 的表达增加。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-06-01 Epub Date: 2024-01-08 DOI: 10.1080/01478885.2023.2301123

Angelin Shanmugam, JoAnna Rudasill, Sheila Criswell

引用次数: 0

Runx1 alleviates osteoarthritis progression in aging mice. Runx1缓解衰老小鼠骨关节炎的进展。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-06-01 Epub Date: 2023-11-15 DOI: 10.1080/01478885.2023.2281790

Haoran Chen, Caixia Pi, Mingyang Chen, Xinmei Du, Yujia Cui, Demao Zhang, Qiang Guo, Jing Xie, Xuedong Zhou

{"title":"Runx1 alleviates osteoarthritis progression in aging mice.","authors":"Haoran Chen, Caixia Pi, Mingyang Chen, Xinmei Du, Yujia Cui, Demao Zhang, Qiang Guo, Jing Xie, Xuedong Zhou","doi":"10.1080/01478885.2023.2281790","DOIUrl":"10.1080/01478885.2023.2281790","url":null,"abstract":"With rates growing quickly with age, osteoarthritis (OA) is the most common cause of chronic disability in aging persons. The discomfort and reduced motion associated with osteoarthritis have a significant impact on quality of life, and there is no known solution. Runt-related transcription factor 1(Runx1) has been shown to play a protective role in the development of osteoarthritis by promoting chondrogenesis. We had created models of ageing mice with osteoarthritis by anterior cruciate ligament transection (ACLT) and analyzed the effects of intra-articular injection of adeno-associated virus/Runx1 (AAV/Runx1) on the models. The results showed that the AAV/Runx1-group maintained better articular cartilage integrity and retained more proteoglycan than the OA group after injection of AAV-Runx1. The markers related to pathological changes in cartilage were downregulated, while the markers related to physiological changes in cartilage were upregulated. This suggests that Runx1 may impede OA progression on the knee joint of ageing mice, potentially playing a protective role in OA and becoming a probable treatment target for osteoarthritis among ageing patients in the future.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"57-67"},"PeriodicalIF":1.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107591455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GW1929 (an agonist of PPARγ) inhibits excessive production of reactive oxygen species in cisplatin-stimulated renal tubular epithelial cells, hampers cell apoptosis, and ameliorates renal injury. GW1929(一种PPARγ激动剂)抑制顺铂刺激的肾小管上皮细胞中活性氧的过量产生，阻碍细胞凋亡，并改善肾损伤。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-06-01 Epub Date: 2023-11-29 DOI: 10.1080/01478885.2023.2286692

Yong He, Caihong Hu, Xin Zhang

{"title":"GW1929 (an agonist of PPARγ) inhibits excessive production of reactive oxygen species in cisplatin-stimulated renal tubular epithelial cells, hampers cell apoptosis, and ameliorates renal injury.","authors":"Yong He, Caihong Hu, Xin Zhang","doi":"10.1080/01478885.2023.2286692","DOIUrl":"10.1080/01478885.2023.2286692","url":null,"abstract":"Cisplatin-induced nephrotoxicity has long been explored for development of preventative and therapeutic drugs. The current investigation focused on the renal protective effect of GW1929, an agonist for peroxisome proliferator-activated receptors gamma (PPARγ), on cisplatin-induced kidney injury. HK2 cells treated with 20 μM cisplatin and C57BL/6 mice injected with 20 mg/kg cisplatin were used as the cell model and animal model for acute kidney injury. HK2 cell viability after cisplatin or GW1929 (0-80 μM) treatment was tested using methyl thiazolyl tetrazolium assays. Flow cytometry analysis and TUNEL assays were used to measure cell apoptosis. Intracellular reactive oxygen species (ROS) level was measured through fluorescence intensities. Levels of blood urea nitrogen (BUN) and serum creatinine (SCr) were measured to evaluate the renal function of mice. For renal morphology observation and cell apoptosis assessment in vivo, hematoxylin-eosin staining and TUNEL assays were conducted. The concentrations of oxidative stress markers in renal samples were measured using colorimetric tests. It was found that GW1929 dose-dependently enhanced protein levels of PPARγ, PGC-1α and TFEB in HK2 cells. Meanwhile, intracellular ROS overproduction, the decrease in cell viability and excessive cell apoptosis mediated by cisplatin were reversed by GW1929. For in vivo experiments, GW1929 notably attenuated cisplatin-stimulated nephrotoxicity and oxidative stress while reducing BUN and Scr levels in cisplatin-challenged model mice. Moreover, GW1929 significantly dampened renal cell apoptosis in vivo. GW1929 mitigates renal tubular epithelial cell injury and renal damage by inhibiting oxidative stress and renal cell apoptosis.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"68-79"},"PeriodicalIF":1.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138451607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A challenging case of sclerosing polycystic adenoma of the parotid gland: a case report and brief review of the latest updates. 一例具有挑战性的腮腺硬化性多囊腺瘤：病例报告和最新进展简评。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-06-01 Epub Date: 2024-01-15 DOI: 10.1080/01478885.2023.2299911

Radwa Rashad, Abdelrahman Barakat

引用次数: 0

Using machine learning for chemical-free histological tissue staining 利用机器学习进行无化学物质组织染色

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-04-22 DOI: 10.1080/01478885.2024.2338585

Julie A. Renner, Patrick C. Riley

引用次数: 0

Molecular surgical pathology, 2nd edition 分子外科病理学，第 2 版

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-04-16 DOI: 10.1080/01478885.2024.2343180

Stephen K. Lau

引用次数: 0

A rapid and simple procedure for the whole-mount bone staining of small fish 小鱼整块骨染色的快速简便程序

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-04-12 DOI: 10.1080/01478885.2024.2339452

Narawadee Yeesan, Chanyut Sudtongkong, Supparat Kong-Oh, Kitipong Angsujinda, Jes Kettratad, Pisit Poolprasert, Atsuo Iida, Piyakorn Boonyoung, Korakot Nganvongpanit, Anan Kenthao, Gen Kaneko, Sinlapachai Senarat

引用次数: 0