Journal of Histotechnology最新文献_第3页

Tissue clearing and three-dimensional imaging of intact tissues: a review on FACT protocol. 完整组织的组织清除和三维成像：FACT 方案综述。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-05-16 DOI: 10.1080/01478885.2024.2352695

Zohreh Farrar, Alireza Afshar, Afshin Zare, Nadiar M Mussin, Asset A Kaliyev, Kulyash R Zhilisbayeva, Mahdi Mahdipour, Amin Tamadon

{"title":"Tissue clearing and three-dimensional imaging of intact tissues: a review on FACT protocol.","authors":"Zohreh Farrar, Alireza Afshar, Afshin Zare, Nadiar M Mussin, Asset A Kaliyev, Kulyash R Zhilisbayeva, Mahdi Mahdipour, Amin Tamadon","doi":"10.1080/01478885.2024.2352695","DOIUrl":"10.1080/01478885.2024.2352695","url":null,"abstract":"FACT is a developed technique for clearing tissues that does not use acrylamide. Since the removal of lipids is crucial for transparency and efficient antibody staining throughout the tissue, especially for microscopy and imaging, it is a harmful process that can cause the loss of important biological molecules such as proteins. The FACT technique overcomes this by chemically bonding the membrane and intracellular proteins with the extracellular matrix, creating a massive 3D hydrogel matrix and providing structural support to fortify the tissue during processing. Compared to other acrylamide-based techniques, the FACT technique requires less labor and harmful chemicals and is therefore considered a more suitable option. In this study, we describe the complete FACT protocol for antibody staining and imaging of whole-cleared tissues while preserving structure and improving image quality. The protocol includes tissue perfusion, fixation, clearing, antibody staining, refractive index matching (RIM) (), microscopy, and imaging. The timing for each step varies depending on the size, weight, and type of tissue, as well as the type of immunostaining. We provide an example of the FACT protocol using mouse brain tissue, which demonstrates its suitability for molecular interrogation analysis of large tissues. The FACT technique has been successfully performed on different types of tissues, making it a favorable choice for a variety of applications.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"126-142"},"PeriodicalIF":0.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140944901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increase of KLK7, cytokeratin 5/6, and elafin expression in head and neck squamous cell carcinoma compared with lung squamous cell carcinoma. 与肺鳞癌相比，头颈部鳞癌中 KLK7、细胞角蛋白 5/6 和 elafin 的表达增加。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-06-01 Epub Date: 2024-01-08 DOI: 10.1080/01478885.2023.2301123

Angelin Shanmugam, JoAnna Rudasill, Sheila Criswell

引用次数: 0

Runx1 alleviates osteoarthritis progression in aging mice. Runx1缓解衰老小鼠骨关节炎的进展。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-06-01 Epub Date: 2023-11-15 DOI: 10.1080/01478885.2023.2281790

Haoran Chen, Caixia Pi, Mingyang Chen, Xinmei Du, Yujia Cui, Demao Zhang, Qiang Guo, Jing Xie, Xuedong Zhou

{"title":"Runx1 alleviates osteoarthritis progression in aging mice.","authors":"Haoran Chen, Caixia Pi, Mingyang Chen, Xinmei Du, Yujia Cui, Demao Zhang, Qiang Guo, Jing Xie, Xuedong Zhou","doi":"10.1080/01478885.2023.2281790","DOIUrl":"10.1080/01478885.2023.2281790","url":null,"abstract":"With rates growing quickly with age, osteoarthritis (OA) is the most common cause of chronic disability in aging persons. The discomfort and reduced motion associated with osteoarthritis have a significant impact on quality of life, and there is no known solution. Runt-related transcription factor 1(Runx1) has been shown to play a protective role in the development of osteoarthritis by promoting chondrogenesis. We had created models of ageing mice with osteoarthritis by anterior cruciate ligament transection (ACLT) and analyzed the effects of intra-articular injection of adeno-associated virus/Runx1 (AAV/Runx1) on the models. The results showed that the AAV/Runx1-group maintained better articular cartilage integrity and retained more proteoglycan than the OA group after injection of AAV-Runx1. The markers related to pathological changes in cartilage were downregulated, while the markers related to physiological changes in cartilage were upregulated. This suggests that Runx1 may impede OA progression on the knee joint of ageing mice, potentially playing a protective role in OA and becoming a probable treatment target for osteoarthritis among ageing patients in the future.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"57-67"},"PeriodicalIF":1.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107591455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GW1929 (an agonist of PPARγ) inhibits excessive production of reactive oxygen species in cisplatin-stimulated renal tubular epithelial cells, hampers cell apoptosis, and ameliorates renal injury. GW1929(一种PPARγ激动剂)抑制顺铂刺激的肾小管上皮细胞中活性氧的过量产生，阻碍细胞凋亡，并改善肾损伤。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-06-01 Epub Date: 2023-11-29 DOI: 10.1080/01478885.2023.2286692

Yong He, Caihong Hu, Xin Zhang

{"title":"GW1929 (an agonist of PPARγ) inhibits excessive production of reactive oxygen species in cisplatin-stimulated renal tubular epithelial cells, hampers cell apoptosis, and ameliorates renal injury.","authors":"Yong He, Caihong Hu, Xin Zhang","doi":"10.1080/01478885.2023.2286692","DOIUrl":"10.1080/01478885.2023.2286692","url":null,"abstract":"Cisplatin-induced nephrotoxicity has long been explored for development of preventative and therapeutic drugs. The current investigation focused on the renal protective effect of GW1929, an agonist for peroxisome proliferator-activated receptors gamma (PPARγ), on cisplatin-induced kidney injury. HK2 cells treated with 20 μM cisplatin and C57BL/6 mice injected with 20 mg/kg cisplatin were used as the cell model and animal model for acute kidney injury. HK2 cell viability after cisplatin or GW1929 (0-80 μM) treatment was tested using methyl thiazolyl tetrazolium assays. Flow cytometry analysis and TUNEL assays were used to measure cell apoptosis. Intracellular reactive oxygen species (ROS) level was measured through fluorescence intensities. Levels of blood urea nitrogen (BUN) and serum creatinine (SCr) were measured to evaluate the renal function of mice. For renal morphology observation and cell apoptosis assessment in vivo, hematoxylin-eosin staining and TUNEL assays were conducted. The concentrations of oxidative stress markers in renal samples were measured using colorimetric tests. It was found that GW1929 dose-dependently enhanced protein levels of PPARγ, PGC-1α and TFEB in HK2 cells. Meanwhile, intracellular ROS overproduction, the decrease in cell viability and excessive cell apoptosis mediated by cisplatin were reversed by GW1929. For in vivo experiments, GW1929 notably attenuated cisplatin-stimulated nephrotoxicity and oxidative stress while reducing BUN and Scr levels in cisplatin-challenged model mice. Moreover, GW1929 significantly dampened renal cell apoptosis in vivo. GW1929 mitigates renal tubular epithelial cell injury and renal damage by inhibiting oxidative stress and renal cell apoptosis.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"68-79"},"PeriodicalIF":1.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138451607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A challenging case of sclerosing polycystic adenoma of the parotid gland: a case report and brief review of the latest updates. 一例具有挑战性的腮腺硬化性多囊腺瘤：病例报告和最新进展简评。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-06-01 Epub Date: 2024-01-15 DOI: 10.1080/01478885.2023.2299911

Radwa Rashad, Abdelrahman Barakat

引用次数: 0

Using machine learning for chemical-free histological tissue staining 利用机器学习进行无化学物质组织染色

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-04-22 DOI: 10.1080/01478885.2024.2338585

Julie A. Renner, Patrick C. Riley

引用次数: 0

Molecular surgical pathology, 2nd edition 分子外科病理学，第 2 版

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-04-16 DOI: 10.1080/01478885.2024.2343180

Stephen K. Lau

引用次数: 0

A rapid and simple procedure for the whole-mount bone staining of small fish 小鱼整块骨染色的快速简便程序

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-04-12 DOI: 10.1080/01478885.2024.2339452

Narawadee Yeesan, Chanyut Sudtongkong, Supparat Kong-Oh, Kitipong Angsujinda, Jes Kettratad, Pisit Poolprasert, Atsuo Iida, Piyakorn Boonyoung, Korakot Nganvongpanit, Anan Kenthao, Gen Kaneko, Sinlapachai Senarat

引用次数: 0

The presence of H. pylori in laparoscopic sleeve gastrectomy specimens is associated with increased mucosal thickness, presence of secondary follicles, increased chronic inflammation, and intestinal metaplasia. 腹腔镜袖状胃切除术标本中幽门螺杆菌的存在与粘膜厚度增加、次级卵泡存在、慢性炎症增加和肠化生有关。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-03-01 Epub Date: 2023-10-12 DOI: 10.1080/01478885.2023.2265601

Danielle Grant, Chelsea Peeler, JoAnna Rudasill, Cynthia Lazar, Amanda Bodkin, Sheila L Criswell

{"title":"The presence of H. pylori in laparoscopic sleeve gastrectomy specimens is associated with increased mucosal thickness, presence of secondary follicles, increased chronic inflammation, and intestinal metaplasia.","authors":"Danielle Grant, Chelsea Peeler, JoAnna Rudasill, Cynthia Lazar, Amanda Bodkin, Sheila L Criswell","doi":"10.1080/01478885.2023.2265601","DOIUrl":"10.1080/01478885.2023.2265601","url":null,"abstract":"Helicobacter pylori is putatively present in over half of the global human population and is recognized as a carcinogenic agent that increases the likelihood of infected patients developing gastric adenocarcinoma or gastric lymphoma. Although there are several means for testing for H. pylori, the gold standard remains the invasive histologic evaluation. The current most popular form of bariatric surgery is the laparoscopic sleeve gastrectomy (LSG) and is the only bariatric surgery which supplies a specimen for histologic evaluation. While non-invasive testing is effective in diagnosing and monitoring H. pylori infection, histological examination of biopsies and resections is the only way to grade chronic inflammation and evaluate specimens for additional pathologies such as intestinal metaplasia. The investigators evaluated 203 sequential LSG specimens collected from a major metropolitan hospital over the period of one year. Specimens were processed to paraffin, stained with hematoxylin and eosin, alcian blue, and immunohistochemistry to determine the presence of H. pylori, chronic inflammation, presence of secondary lymphoid follicles in the mucosa, mucosal thickness, and presence of intestinal metaplasia. Statistical analyses demonstrated a significant positive correlation among all factors examined. The overall positivity rate of H. pylori in LSG specimens was 18.2% but ranged from 6.9-23.8% depending on whether the treating clinician performed routine pre-surgical endoscopy. The presence of H. pylori was associated with a higher average chronic inflammation grade, intestinal metaplasia, thicker mucosa, and presence of lymphoid follicles with germinal centers in the mucosa.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"5-12"},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41203065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histomorphogenesis of sublingual salivary gland of Indian sheep. 印度绵羊舌下唾液腺的组织形态发生。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-03-01 Epub Date: 2023-10-24 DOI: 10.1080/01478885.2023.2266799

Amandeep Singh, Simran Kaur, Opinder Singh

{"title":"Histomorphogenesis of sublingual salivary gland of Indian sheep.","authors":"Amandeep Singh, Simran Kaur, Opinder Singh","doi":"10.1080/01478885.2023.2266799","DOIUrl":"10.1080/01478885.2023.2266799","url":null,"abstract":"The primordial anlage of sublingual gland was first noticed as a solid epithelial bud from oral epithelium at the 24th day of foetal development. The terminal buds were arranged in the form of clusters with undifferentiated epithelial cells and terminated in a bulb-like structure in the 30-day-old sheep foetus. On the 37th day, lumenization and branching of the main cord was noticed. The primary septa were observed from the 55th day onwards which resulted in the formation of lobulation on the 60th day. The capsule formation was initiated by aggregation of mesenchymal tissue on the 63rd day. On the 100th day, terminal tubules differentiated to form secretory end pieces. Tubular portions formed intercalated and striated ducts. Predominantly mucous type of acinar cells was seen from the 110th day onwards with myoepithelial cells. The number of lobules increased with increase in parenchyma from 130th day onwards. Micrometrical studies revealed that the mean diameter of acini, intercalated, striated and large ducts was increased with advancement of age and significant differences were observed between groups. Localization of acidic and neutral mucopolysaccharides were observed in mucous and goblet cells. Fine lipid droplets were observed in intralobular and interlobular connective tissue however, phospholipids were observed in cell membrane of acini and ducts. The current investigation provides microstructural standards for the organogenesis of the sublingual gland of miniature sheep and can lay the foundation for further studies in the morphological investigation of salivary gland development.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"13-22"},"PeriodicalIF":1.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49690848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0