Journal of Histotechnology最新文献_第2页

Comparative analysis of in vivo bio-integration of three hyaluronic acid-based fillers for 26 weeks: a histological study. 三种透明质酸类填充剂 26 周体内生物整合比较分析：组织学研究。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-01 Epub Date: 2024-07-18 DOI: 10.1080/01478885.2024.2369967

Baoji Song, Qiqi Chen

引用次数: 0

Novel processing and staining methodology of bottlenose dolphin (Tursiops truncatus) teeth for age determination. 用于年龄测定的瓶鼻海豚（Tursiops truncatus）牙齿新处理和染色方法。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-01 Epub Date: 2024-03-11 DOI: 10.1080/01478885.2024.2327155

Christa E Barrett, Ian Evans, Timothy Morgan, Alicia K Olivier

{"title":"Novel processing and staining methodology of bottlenose dolphin (Tursiops truncatus) teeth for age determination.","authors":"Christa E Barrett, Ian Evans, Timothy Morgan, Alicia K Olivier","doi":"10.1080/01478885.2024.2327155","DOIUrl":"10.1080/01478885.2024.2327155","url":null,"abstract":"Age determination of bottlenose dolphins (Tursiops truncatus) is a critical tool in understanding both individual and population health. There are many methods of aging bottlenose dolphins including analysis of teeth, pectoral flipper radiographs, and epigenetics. The most common and oldest method for aging toothed cetaceans is the counting of growth layer groups (GLGs) in the teeth. Current techniques have technical and repeatability challenges. Therefore, a processing technique that results in better resolution of GLGs is needed. This study compares different decalcifications and different histochemical staining techniques. Decalcification was done using 10% EDTA, Kristensen's decalcification, and Rapid Decalcification Solution (RDO). Following decalcification and routine processing, GLGs were assessed using Hematoxylin and Eosin (H&E), hematoxylin, Giemsa, Wright-Giemsa, Toluidine Blue (T-Blue), Masson's Trichrome, and Congo Red staining techniques. Decalcification with Kristensen's and staining with Masson's Trichrome and Congo Red were determined to best highlight GLGs. This processing and staining was then applied to a sample population of 102 bottlenose dolphins that were evaluated independently and blindly by two observers. Of the 102 dolphin samples, 13 (12.7%) were unable to age due to no clear distinction or distortion between GLGs.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"146-153"},"PeriodicalIF":0.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Addressing the histotechnologist shortage through improved classification and recognition. 通过改进分类和识别，解决组织技术人员短缺问题。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI: 10.1080/01478885.2024.2424049

Henley Marcelus, Daniel Packert

引用次数: 0

Low-magnitude high-frequency vibration ameliorates high glucose-induced endothelial injury by restoring mitochondrial function via AMPK/mTOR pathway. 低幅高频振动通过 AMPK/mTOR 途径恢复线粒体功能，从而改善高血糖诱导的内皮损伤。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-11-20 DOI: 10.1080/01478885.2024.2429855

Xidan Zhang, Jiyu Sun, Xiting Zhu, Zhenghao Yang, Zhuoli Zhu, Min Zhou, Chen Li, Haiyang Yu, Xueqi Gan

{"title":"Low-magnitude high-frequency vibration ameliorates high glucose-induced endothelial injury by restoring mitochondrial function via AMPK/mTOR pathway.","authors":"Xidan Zhang, Jiyu Sun, Xiting Zhu, Zhenghao Yang, Zhuoli Zhu, Min Zhou, Chen Li, Haiyang Yu, Xueqi Gan","doi":"10.1080/01478885.2024.2429855","DOIUrl":"https://doi.org/10.1080/01478885.2024.2429855","url":null,"abstract":"High glucose-induced dysfunction of endothelial cells is a critical and initiating factor in the genesis of diabetic vascular complications. Low-magnitude high-frequency vibration (LMHFV) is a non-invasive biophysical intervention. It has been reported that it exhibits protective effects on high glucose-induced osteoblast dysfunction, but little was known on diabetic vascular complications. In this work, we aim to clarify the role of LMHFV on high glucose-induced endothelial dysfunction and hypothesized that the protective effects functioned through adenosine monophosphate-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway. We cultured primary murine aortic endothelial cells (MAECs) in normal or HG medium, respectively, before exposing to LMHFV. The tube formation, paracellular permeability assay, and aortic ring sprouting assay showed that the high glucose injured-function of MAECs was improved after LMHFV treatment. The intracellular ROS generation analysis, mitochondrial complex I activities measurement, ATP measurement and mitochondrial membrane potential (MMP), and mitochondrial ROS generation analysis of MAECs indicated that mitochondrial function was restored by LMHFV loading in a high glucose environment. Mechanically, western blot assays showed that AMPK phosphorylation was promoted and mTOR was inhibited in LMHFV-induced endothelial function restoration. After the administration of the AMPK inhibitor, Compound C, these protective effects resulting from LMHFV are reversed. These findings suggest that LMHFV plays a significant role in protecting endothelial cells' function and mitochondrial function in high glucose-induced injured MAECs via AMPK/mTOR signalling.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":0.6,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic yield of stains for infectious organisms in esophageal or gastroesophageal junction biopsies with esophagitis. 食管炎食管或胃食管交界处活检中感染性生物体染色的诊断率。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-10-14 DOI: 10.1080/01478885.2024.2415154

Aaron R Huber, Christa L Whitney-Miller

引用次数: 0

The impact of gliomas on the normal brain microenvironment: a pilot study. 胶质瘤对正常大脑微环境的影响：一项试点研究。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-10-01 DOI: 10.1080/01478885.2024.2408505

Nicole Riha, Jacen S Moore, Sheila Criswell

{"title":"The impact of gliomas on the normal brain microenvironment: a pilot study.","authors":"Nicole Riha, Jacen S Moore, Sheila Criswell","doi":"10.1080/01478885.2024.2408505","DOIUrl":"https://doi.org/10.1080/01478885.2024.2408505","url":null,"abstract":"Gliomas are malignant tumors of neuronal support cells within the central nervous system (CNS) and are characterized by poor overall prognoses and limited treatment options due to their infiltrative growth patterns. The neural tumor microenvironment, composed of benign neurons, neuroglia, endothelial cells, and intravascular white blood cells, is a target-rich site for potential chemotherapeutic agents. This study assessed cell proliferation rates, white blood cell components, and a limited number of nuclear, cytoplasmic, and membrane markers using immunohistochemistry (IHC) assays on formalin-fixed and paraffin-embedded benign and glial tumor tissue samples from the CNS. It was observed that glioma tissues had increased rates of glial cell proliferation and significant increases in the number of observed T-lymphocytes and granulocytes but decreased expression of markers Somatostatin receptor 2 (SSTR2), L1 cell adhesion molecule (L1CAM), and GATA binding protein 3 (GATA3) when compared to benign tissue samples. Understanding the lack of protein expression and population expansion potential of the glioma microenvironment in greater detail could help identify valuable therapeutic target combinations for future treatments.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":0.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptation of the HistoEnder, an open-source 3D printer for automated transmission electron microscopy grid staining. 对用于自动透射电子显微镜网格染色的开源三维打印机 HistoEnder 进行改装。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-23 DOI: 10.1080/01478885.2024.2403872

Philip Seifert

引用次数: 0

A study of PGP 9.5 immunohistochemical labeling on formalin-fixed paraffin embedded tissues for epidermal nerve fiber density testing 对用于表皮神经纤维密度测试的福尔马林固定石蜡包埋组织进行 PGP 9.5 免疫组化标记的研究

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-09-17 DOI: 10.1080/01478885.2024.2405418

Joseph A. Esposito, Camryn J. Vader, Joel R. Israel, Steve A. McClain

引用次数: 0

Survey of NAACLS accredited histotechnology programs in the United States. 美国经 NAACLS 认证的组织技术课程调查。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-03-18 DOI: 10.1080/01478885.2024.2327095

Carol Bain, Debra Wood, Sheila Criswell

{"title":"Survey of NAACLS accredited histotechnology programs in the United States.","authors":"Carol Bain, Debra Wood, Sheila Criswell","doi":"10.1080/01478885.2024.2327095","DOIUrl":"10.1080/01478885.2024.2327095","url":null,"abstract":"Histotechnology educational programs are accredited by the National Accrediting Agency for Clinical Laboratory Sciences (NAACLS) and currently number fewer than 50 in the United States which contributes to the shortages of laboratory personnel. A survey tool designed with REDCap software was distributed to all programs identified on the NAACLS website and consisted of three parts: a) program information, b) facility information, and c) challenges. Programs are located primarily in large urban centers where populations are most concentrated. The median class size was 6 which may explain the excellent student outcomes to include 96% graduation rates and 90.7% board of registry examination pass rates. Overall, programs had ample equipment, funding, and administrative support. Costs to attend the programs were relatively low (<$3,000 per semester) for over half of the programs. However, due to the small number of accredited education programs across the US, potential students do not often have access to an institution in their area. The programs indicated that the most common challenge was recruitment of adequate high-quality candidates which may explain, in part, the persistent shortage of personnel in the histology laboratory.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"109-116"},"PeriodicalIF":0.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140143574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of light and electron microscopic morphometric parameters of glomerular capillaries with serum creatinine and proteinuria. 肾小球毛细血管的光镜和电子显微镜形态参数与血清肌酐和蛋白尿的相关性。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-03-11 DOI: 10.1080/01478885.2024.2326274

Dibyajyoti Boruah, A W Kashif, Barun Kumar Chakrabarty, Sarika Harikrishnan, Arijit Sen

{"title":"Correlation of light and electron microscopic morphometric parameters of glomerular capillaries with serum creatinine and proteinuria.","authors":"Dibyajyoti Boruah, A W Kashif, Barun Kumar Chakrabarty, Sarika Harikrishnan, Arijit Sen","doi":"10.1080/01478885.2024.2326274","DOIUrl":"10.1080/01478885.2024.2326274","url":null,"abstract":"Waste products in the bloodstream are filtered by the glomerular capillaries in the kidneys and excreted into the urine. When making a differential diagnosis of kidney diseases, structural assessment of glomeruli using histological, ultrastructural, and immunological studies is crucial. This study assessed the microscopic and ultrastructural morphometric parameters of glomerular capillaries and examined their correlation with serum creatinine and proteinuria. A total of 60 kidney biopsy cases received by the transmission electron microscope (TEM) laboratory for diagnosis were included in the study. Toluidine blue stained 300 nm thick sections of TEM tissue blocks were scanned for glomerular morphometry by a whole slide imaging system, and the estimation of Bowman's capsule (BC) area, glomerular capillary lumen diameter (GCLD), glomerular capillary density (GCD), glomerular capillary surface area density (GCSA), and percentage of glomerular capillary lumen space (%GCLS) was performed with QuPath software. TEM images of 70 nm thick sections were used for the evaluation of endothelial fenestration diameter (EFD), glomerular basement membrane (GBM) thickness, and podocyte foot process (PFP) effacement. Proteinuria and serum creatinine showed positive correlations with GBM thickness and PFP effacement. Negative correlations of serum creatinine were observed with EFD, %GCLS, and GCSA. Hence, glomerular filtration is greatly affected by the total area of the glomerular capillary surface and structural changes of GBM. Reduction of glomerulus filtration due to foot process effacement and thickening of GBM results in damage to the filtration barrier leading to the leakage of plasma protein into urine.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"97-108"},"PeriodicalIF":0.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0