Journal of Histotechnology最新文献_第2页

An automatic, rapid and accurate method for the annotation of tumor components on whole slide images. 一种自动、快速、准确的肿瘤成分全切片标注方法。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2026-03-01 Epub Date: 2026-01-02 DOI: 10.1080/01478885.2025.2602268

Hong Tang, Xiaodong Wang, Xiaolin Zhang, Xiaojun Wu, Xinyue Tang, Yaqiong Ma, Ying Chen, Guanzhen Yu

{"title":"An automatic, rapid and accurate method for the annotation of tumor components on whole slide images.","authors":"Hong Tang, Xiaodong Wang, Xiaolin Zhang, Xiaojun Wu, Xinyue Tang, Yaqiong Ma, Ying Chen, Guanzhen Yu","doi":"10.1080/01478885.2025.2602268","DOIUrl":"10.1080/01478885.2025.2602268","url":null,"abstract":"Annotated pathology datasets are the cornerstone for developing computational pathology. However, the intrinsic complexity of pathology data often results in a scarcity of large, manually annotated datasets. To address this challenge, a rapid, accurate, and automatic method for annotating tumor cells is essential for advancing the field of computational pathology. Here, we introduce a novel annotation technology. In our approach, Hematoxylin-eosin (H&E) slides were first digitized and preserved. These H&E slides were then faded and re-stained using multiple biological technologies (MBT) to identify specific biomarkers. The re-stained MBT slides were subsequently scanned again to create new digital images. The original and re-stained digital images underwent a registration and segmentation process to ensure that all minute structures in both images aligned perfectly. The staining results from the MBT slides, referred to as2 label maps, were extracted and transferred back onto the H&E slides, and the data were merged. This method allows for precise and efficient annotation of all tumor cells, including those expressing specific biomarkers, as well as the components of the tumor microenvironment. By rapidly generating high-quality, high-volume datasets, this innovative method significantly enhances the ability of AI approaches to analyze and interpret pathology data. Consequently, it supports the development of highly accurate diagnostic, prognostic, and predictive decision-making systems in the field of computational pathology.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"13-25"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145889283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automatic cell classification and quantification with machine learning in immunohistochemistry images. 免疫组织化学图像中的机器学习自动细胞分类和定量。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2026-03-01 Epub Date: 2025-07-01 DOI: 10.1080/01478885.2025.2523618

Pikting Cheung, Wei Zhang, Muhammad Shehzad Khan, Irfan Ahmed, Yuanchao Liu, Fraser Hill, Xinyue Li, Condon Lau

{"title":"Automatic cell classification and quantification with machine learning in immunohistochemistry images.","authors":"Pikting Cheung, Wei Zhang, Muhammad Shehzad Khan, Irfan Ahmed, Yuanchao Liu, Fraser Hill, Xinyue Li, Condon Lau","doi":"10.1080/01478885.2025.2523618","DOIUrl":"10.1080/01478885.2025.2523618","url":null,"abstract":"The incidence of lymphoma, a cancer that affects both humans and animals, has witnessed a significant increase. In response, immunohistochemistry (IHC) has become an essential tool for its classification. This prompted us to develop an innovative mathematical methodology for the precise quantification of immunopositive and immunonegative cells, along with their spatial analysis, in CD3-stained lymphoma IHC images. Our approach involves integrating an algorithm based on a mathematical color model for cell differentiation, employing the distinctive morphological erosion, algorithmic transformations, and customized histogram equalization to enhance features. Refined local thresholding enhances classification precision. Additionally, a customized circular Hough transform quantifies cell counts and assesses their spatial data. The algorithms accurately enumerate cell types, reducing human intervention and providing total numbers and spatial information on detected cells within tissue specimens. Evaluation of IHC image samples revealed an overall accuracy of 93.98% for automatic cell counts. The automatic counts and location information were cross-validated by three pathology specialists, highlighting the effectiveness and reliability of our automated approach. Our innovative framework enhances lymphoma cell counting accuracy in IHC images by combining physics-based color understanding with machine learning, thereby improving diagnosis and reducing the risks of human error.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"26-43"},"PeriodicalIF":1.8,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of decalcification solutions on cellular morphology and immunostaining of mouse bones. 脱钙溶液对小鼠骨细胞形态及免疫染色的影响。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-12-01 Epub Date: 2025-11-12 DOI: 10.1080/01478885.2025.2583646

Madison Toonder, Skyler R Turner, Rachel Howie, Avi Barlatier, Miranda K Wilkes, Cindy Lowe, Tzushan S Yang, Katherine N Gibson-Corley

{"title":"Assessment of decalcification solutions on cellular morphology and immunostaining of mouse bones.","authors":"Madison Toonder, Skyler R Turner, Rachel Howie, Avi Barlatier, Miranda K Wilkes, Cindy Lowe, Tzushan S Yang, Katherine N Gibson-Corley","doi":"10.1080/01478885.2025.2583646","DOIUrl":"10.1080/01478885.2025.2583646","url":null,"abstract":"Decalcification is an important step in histology laboratories to allow mineralized tissue samples to be trimmed and sectioned easily. Many decalcifying solutions have a rapid onset of action in softening tissues but alter protein structure and morphology, while others preserve protein integrity but are less efficient. The ideal decalcification protocol allows for rapid and cost-effective processing and precise evaluation of microscopy and antigen-based immunostaining such as immunohistochemistry. In our study, mouse tissues were decalcified with three commercially available solutions to identify the product that best meets those criteria. ImmunocalTM (StatLab), EprediaTM, and Rapid-CalTM (StatLab) decalcification agents were tested on formalin-fixed, paraffin-embedded CD-1 mouse femur, skull, and sternum samples. Multiple metrics including ammonium oxalate turbidity and radiography were used to assess stages of bone demineralization. Tissues were routinely processed, embedded in paraffin, and sectioned at 5 µm for H&E staining and CD3, CD31, and Iba1 immunostaining. Each tested sample represented a decalcification product and time (4, 6, 12, 24, 30, 48, 73 h). Samples were assessed by radiolucency on X-ray and gross bone pliability prior to histologic processing, followed by histopathologic scoring for completeness of demineralization, preservation of tissue architecture, and antigenicity of tissue. All three commercially available decalcifying solutions are sufficient for rapid decalcification with preservation of tissue integrity, cellular detail, and immunogenicity.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"168-177"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145495636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathological analysis of Artemia franciscana nauplii under different forms of mercury stress. 不同形式汞胁迫下金翅蒿的组织病理学分析。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-12-01 Epub Date: 2025-07-02 DOI: 10.1080/01478885.2025.2523622

Yanlin Wei, Wei Liu, Fengling Zhang, Yang Chen, Dongxin Wei, Hangyu Lin, Tao He

{"title":"Histopathological analysis of Artemia franciscana nauplii under different forms of mercury stress.","authors":"Yanlin Wei, Wei Liu, Fengling Zhang, Yang Chen, Dongxin Wei, Hangyu Lin, Tao He","doi":"10.1080/01478885.2025.2523622","DOIUrl":"10.1080/01478885.2025.2523622","url":null,"abstract":"Inorganic mercury (Hg) and methylmercury (MeHg) have emerged as global pollutants owing to their long-term environmental stability and bioaccumulation. These heavy metals enter aquatic systems via industrial emissions, coal combustion, and natural processes, posing a serious threat to ecosystems and human health. This study assesses the impact of Hg2+ and MeHg on the growth and development of brine shrimp (Artemia franciscana) nauplii by analyzing the histopathological effects on their tissues. Brine shrimp nauplii from the Bohai Bay in China were selected as the study subjects and exposed to 1 μmol/L solutions of HgCl₂ and MeHgCl. Tissue sections were continuously taken at different immersion times. After staining with the standard hematoxylin-eosin (HE) method, the tissue morphology of brine shrimp nauplii under different forms of Hg stress was observed under a light microscope. The results showed that MeHg exhibited significantly greater toxicity to brine shrimp nauplii than Hg2+. Under the same exposure time, the MeHg group exhibited more pronounced epithelial cell damage, nuclear material disorder, and nucleoplasm diffusion outside the nucleus than the HgCl₂ group. This finding provides an important theoretical support for further research into the toxicological mechanisms of MeHg and Hg2+, and highlights that the toxic effects of methylmercury on aquatic organisms.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"178-190"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comprehensive panel of testing for amyloidosis. 淀粉样变性的综合检查。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-12-01 Epub Date: 2025-09-10 DOI: 10.1080/01478885.2025.2553926

Hong Tang, Feifei Zhao, Yingwei Zhu, Rongrong Xu, Huiqing Yuan, Min Xie, Rui Wu

{"title":"A comprehensive panel of testing for amyloidosis.","authors":"Hong Tang, Feifei Zhao, Yingwei Zhu, Rongrong Xu, Huiqing Yuan, Min Xie, Rui Wu","doi":"10.1080/01478885.2025.2553926","DOIUrl":"10.1080/01478885.2025.2553926","url":null,"abstract":"Amyloidosis encompasses a spectrum of rare disorders characterized by extracellular amyloid deposition. Achieving an accurate early diagnosis of systemic amyloidosis necessitates biopsy-specific pathological evaluation. Formalin-fixed, paraffin-embedded liver biopsy specimens were examined using Congo red staining, electron microscopy, immunohistochemistry (IHC), immunofluorescence, and Congo red-assisted laser microdissection with mass spectrometry (LMD/MS). Failure Mode and Effects Analysis (FMEA) was employed for risk mitigation and quality control. Classical Congo red staining exhibited brick-red coloration, enhanced alkalinization, reduced permanganate staining, and characteristic apple-green birefringence under polarized light. Trypsin-digested IHC demonstrated kappa light chain positivity and lambda negativity, with improved background clarity compared to other retrieval methods-results concordant with electron microscopic colloidal gold staining, albeit with higher tissue consumption. Congo red-polarized microscopy permitted direct amyloid deposit localization. Subsequent LMD/MS identified immunoglobulin kappa light chain as the pathogenic precursor protein, though at increased expense. Congo red staining under polarized light remains the cornerstone technique for amyloid detection. LMD/MS provides superior specificity in amyloid typing relative to IHC, immunofluorescence, or electron microscopy, proving particularly advantageous for limited samples. Traditional methods remain valuable for validation when tissue is abundant. Histopathological assessment continues to be the diagnostic gold standard for hepatic amyloidosis; systematic integration and analytical refinement of these techniques are imperative for enhancing diagnostic accuracy.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"191-199"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chinese expert opinion on AI-based identification of lymph node metastases in solid tumors. 基于人工智能识别实体瘤淋巴结转移的中国专家意见。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-12-01 Epub Date: 2025-11-10 DOI: 10.1080/01478885.2025.2580157

Xiaodong Wang, Hong Tang, Ying Chen, Xiaolin Zhang, Xiyang Liu, Minghua Zhu, Canrong Ni, Guanzhen Yu

引用次数: 0

Calcification density in single and twin placenta villi. 单胎座绒毛和双胎座绒毛钙化密度。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-12-01 Epub Date: 2025-09-10 DOI: 10.1080/01478885.2025.2557645

Maggie Guess, Sheila Criswell

{"title":"Calcification density in single and twin placenta villi.","authors":"Maggie Guess, Sheila Criswell","doi":"10.1080/01478885.2025.2557645","DOIUrl":"10.1080/01478885.2025.2557645","url":null,"abstract":"Placentas are temporary organs needed to support a developing embryo and arise from both embryonic and maternal tissues. Calcifications of tissues outside of bone and teeth mineralization are often a sign of tissue damage and impaired organ function. Placental calcifications have been described previously in the literature and usually increase in normal pregnancies as the placenta ages, but they have also been associated with the potential for fetal distress. This study utilized 139 placental tissues from singleton control placentas (51), fused twin placentas (48), and non-fused twin placentas (40) with weights over 320 grams (third trimester) and similar maternal ages to determine which placental type(s) exhibited the largest density of calcifications using the von Kossa stain for calcium salts. The study found there were no differences in calcification densities among placenta types, suggesting that twin pregnancies do not experience additional placental stress risk from calcifications. Importantly, the finding that nearly all third-trimester placentas contained calcifications when systematically evaluated indicates that calcification may represent a normal maturational process rather than a pathologic sign of distress. These results help to caution the interpretation of placental calcifications and may provide reassurance to patients and providers managing twin pregnancies.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"200-207"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145029968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiple levels of Gomori methenamine silver (GMS) stains do not improve diagnostic yield in esophageal biopsies. 高水平的戈莫里甲基苯丙胺银（GMS）染色不能提高食管活检的诊断率。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-12-01 Epub Date: 2024-12-12 DOI: 10.1080/01478885.2024.2437587

Michael Occidental, Andrew Dunn, Aaron R Huber

引用次数: 0

Immunohistochemical localization of glial fibrillary acidic protein in the retinas of some amphibian, reptilian, birds, and mammals species. 一些两栖动物、爬行动物、鸟类和哺乳动物视网膜胶质原纤维酸性蛋白的免疫组织化学定位。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-11-03 DOI: 10.1080/01478885.2025.2582277

Amin A Seleem

{"title":"Immunohistochemical localization of glial fibrillary acidic protein in the retinas of some amphibian, reptilian, birds, and mammals species.","authors":"Amin A Seleem","doi":"10.1080/01478885.2025.2582277","DOIUrl":"https://doi.org/10.1080/01478885.2025.2582277","url":null,"abstract":"Glial fibrillary acidic protein (GFAP) immunohistochemistry staining is specific to glial cell intermediate filaments. GFAP has been reported in some diseases related to injury of central nervous system. The actual normal biological function of GFAP in the retinas of some vertebrates is still under investigation. The current study examines the distribution of GFAP in the retinas of various species of amphibian, reptilian, birds, and mammals by immunohistochemical technique. The results indicated that GFAP localization in the retina is related to specific species. Unique, homogeneous GFAP labelling was observed in Chalcides ocellatus retina, which was different from other studied reptiles (Trachemys scripta elegans, Uromastyx aegyptius, Ptyodactylus hasselquistii, Acanthodactylus boskianus, Scincus mitranus). Low GFAP labelling was noted in the retinas of birds and some species of the studied mammals (Jaculus jaculus, Rousettus egyptiacus, and Mesocricetus auratus). However, other studied mammals (Mus musculus, Acomys russatus, Paraechinus aethiopicus) showed different intensities of GFAP expression. The study finds that GFAP helps to clarify the potential function of Müller cells in the regeneration process in different species.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-14"},"PeriodicalIF":1.8,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145438297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphometric study of bioprinted hydrogel deformation during histological preparation. 生物打印水凝胶在组织学制备过程中变形的形态计量学研究。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-09-09 DOI: 10.1080/01478885.2025.2539624

Lucie Essayan, Alexandre Dufour, Emma Petiot, Christophe Marquette

{"title":"Morphometric study of bioprinted hydrogel deformation during histological preparation.","authors":"Lucie Essayan, Alexandre Dufour, Emma Petiot, Christophe Marquette","doi":"10.1080/01478885.2025.2539624","DOIUrl":"https://doi.org/10.1080/01478885.2025.2539624","url":null,"abstract":"Histological preparation via paraffin embedding is the gold standard method for evaluating tissue structure and composition, whether it is originated from biopsy or engineered in vitro. Quite often, deformation and shrinkage occur during the histological preparation, which are difficult to predict and qualify. The present study investigates the morphometric changes in bioprinted hydrogels composed of alginate and gelatine, common tissue engineering materials, focusing on three morphologies: full slabs, porous slabs, and porous cubes. These structures underwent key histological steps, including fixation, processing (dehydration, clearing, and infiltration with melted paraffin), embedding, and slicing, to evaluate their shrinkage behavior. Shrinking factors were systematically measured, showing that processing had the most significant effect (34-40% shrinking), followed by fixation (20-28% shrinking). Porous structures exhibited greater shrinkage compared to full slabs due to their internal geometry. Additionally, anisotropic behavior was observed in porous cubes, with different shrinking factors in the XY plane (horizontal) and Z direction (vertical), leading to an overall volumetric shrinking factor of 81.3%. The results demonstrated the critical influence of hydrogel structure on deformation and emphasized the need for tailored histological protocols to maintain structural fidelity. While this study focused on hydrogels alone, future work will incorporate cellularized bioengineered tissues to evaluate the impact of cell-mediated remodeling and extracellular matrix deposition on histological outcomes. This research offers a framework for optimizing histological preparation in bioengineered tissues, enabling more accurate assessment of their structure and function for regenerative medicine applications.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":1.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0