Journal of Histotechnology最新文献_第2页

Histogel-based techniques for embedding organoids in paraffin blocks enable high throughput downstream histopathological analyses. 基于组织凝胶的技术可将有机体包埋在石蜡块中，从而实现高通量的下游组织病理学分析。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-03-01 Epub Date: 2024-09-05 DOI: 10.1080/01478885.2024.2398381

Charles Havnar, Loryn Holokai, Ryan Ichikawa, Wennie Chen, Alexis Scherl, Eliah R Shamir

{"title":"Histogel-based techniques for embedding organoids in paraffin blocks enable high throughput downstream histopathological analyses.","authors":"Charles Havnar, Loryn Holokai, Ryan Ichikawa, Wennie Chen, Alexis Scherl, Eliah R Shamir","doi":"10.1080/01478885.2024.2398381","DOIUrl":"10.1080/01478885.2024.2398381","url":null,"abstract":"Organoids are in vitro tissue models derived from human or animal primary tissues or stem cells that allow for studying three-dimensional (3D) tissue biology, toxicity testing, biomarker evaluation, and assessment of compound efficacy, supplementing or potentially minimizing use of animal models. Organoids are typically cultured in a 3D format within an extracellular matrix and, at the end of an experiment, can be further processed for various cellular or molecular readouts. Analysis often relies on whole mount immunolabeling for markers of interest, which consumes the entire sample/well, thereby limiting sample availability for downstream assays. In addition, 3D cultures become more friable after fixation and are susceptible to sample loss during washing steps. In contrast, by fixing and processing organoids to a paraffin block, dozens or hundreds of unstained slides can be generated, enabling robust characterization via multiple assays, including histologic evaluation and (immuno)histochemical stains, thus maximizing the yield of these time- and labor-intensive cultures. Here we describe three methods to process 3D Matrigel cultures into paraffin blocks using Histogel as an embedding agent. The three techniques all yield high-quality sections but vary in complexity of implementation at different steps, and their application for different use cases is discussed.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"46-57"},"PeriodicalIF":0.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological methods for plant tissues. 植物组织的组织学方法。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-03-01 Epub Date: 2024-09-02 DOI: 10.1080/01478885.2024.2397989

Sheila Criswell, Brian Gaylord, Christopher R Pitzer

{"title":"Histological methods for plant tissues.","authors":"Sheila Criswell, Brian Gaylord, Christopher R Pitzer","doi":"10.1080/01478885.2024.2397989","DOIUrl":"10.1080/01478885.2024.2397989","url":null,"abstract":"Although many of the structures and organelles of vegetative cells are comparable to those of animal tissues, significant differences between the two kingdoms require modifications in histological techniques for both tissue processing steps and histochemical staining techniques. The authors investigated the challenges of working with plant tissues by collecting various flora to represent the four main plant organs: leaf, stem, root, and flower/fruit. Triplicate samples for each specimen were placed into formalin for paraffin embedding, placed into formalin for later frozen sections, and used fresh to undergo immediate frozen sectioning. Frozen sections of plant tissues were more difficult to obtain than formalin-fixed paraffin-embedded (FFPE) sections, exhibited tissue loss during staining, and were inferior morphologically to FFPE sections. Although, historically, plant tissue fixation and processing has employed several different reagents compared with those used in animal tissue processing and took significantly longer times, the current investigation determined reagents and protocols from a modern histology laboratory which processes mammalian tissues can be applied to plant tissue processing with only slight modifications in respect to reagent timing. Additionally, staining techniques were compared and while it is well known that plant cell walls stain well with safranin O, the current investigation determined the uptake of safranin O can be accelerated by incubating at 60°C.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"58-67"},"PeriodicalIF":0.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resolving the bone - optimizing decalcification in spatial transcriptomics and molecular pathology. 在空间转录组学和分子病理学中解决骨优化脱钙问题。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-03-01 Epub Date: 2024-12-26 DOI: 10.1080/01478885.2024.2446038

Shuoshuo Wang

引用次数: 0

Histotechnology at the crossroad of spatial biology: bridging legacy and innovation. 空间生物学十字路口的组织技术：弥合遗产与创新。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-03-01 Epub Date: 2025-02-04 DOI: 10.1080/01478885.2025.2456415

Shuoshuo Wang, Yongfu Wang, David Krull

引用次数: 0

C5aR expression in kidney tubules, macrophages and fibrosis. 肾小管、巨噬细胞和纤维化中的 C5aR 表达。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-03-01 Epub Date: 2024-11-28 DOI: 10.1080/01478885.2024.2430041

Carolyn Dunlap, Niky Zhao, Linda S Ertl, Thomas J Schall, Kathleen M C Sullivan

{"title":"C5aR expression in kidney tubules, macrophages and fibrosis.","authors":"Carolyn Dunlap, Niky Zhao, Linda S Ertl, Thomas J Schall, Kathleen M C Sullivan","doi":"10.1080/01478885.2024.2430041","DOIUrl":"10.1080/01478885.2024.2430041","url":null,"abstract":"The anaphylatoxin C5a and its receptor C5aR (CD88) are complement pathway effectors implicated in renal diseases, including ANCA-associated vasculitis. We investigated the kidney expression of C5aR and a second C5a receptor C5L2 by using immunohistochemistry, in situ hybridization, and spatial gene expression on formalin-fixed, paraffin-embedded human and mouse kidney. C5aR was detected on interstitial macrophages and in multiple tubular regions, including distal and proximal; C5L2 had a similar expression pattern. The 5/6 nephrectomy model of chronic kidney injury exhibited increased C5aR expression by infiltrating cells within the fibrotic regions. C5aR expression was confirmed on human leukocytes and in vitro differentiated macrophages by flow cytometry, and treatment with C5a induced the expression of chemokines and remodeling factors by macrophages, including CCL-3/-4/-7, -20, MMP-1/-3/-8/-12, and F3, and promoted leukocyte survival. C5a activity was C5aR dependent, as demonstrated by reversal with the C5aR inhibitor avacopan. Collectively, these results suggest that myeloid C5aR may induce excessive inflammation in the kidney via immune cell recruitment, extracellular matrix destruction, and remodeling, resulting in fibrotic tissue deposition.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"27-45"},"PeriodicalIF":0.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A best practices framework for spatial biology studies in drug discovery and development: enabling successful cohort studies using digital spatial profiling. 药物发现和开发中空间生物学研究的最佳实践框架：利用数字空间剖析成功开展队列研究。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-03-01 Epub Date: 2024-09-03 DOI: 10.1080/01478885.2024.2391683

David Krull, Premi Haynes, Anil Kesarwani, Julien Tessier, Benjamin J Chen, Kelly Hunter, Deniliz Rodriguez, Yan Liang, Jim Mansfield, Maxine McClain, Corinne Ramos, Edward Bonnevie, Esperanza Anguiano

{"title":"A best practices framework for spatial biology studies in drug discovery and development: enabling successful cohort studies using digital spatial profiling.","authors":"David Krull, Premi Haynes, Anil Kesarwani, Julien Tessier, Benjamin J Chen, Kelly Hunter, Deniliz Rodriguez, Yan Liang, Jim Mansfield, Maxine McClain, Corinne Ramos, Edward Bonnevie, Esperanza Anguiano","doi":"10.1080/01478885.2024.2391683","DOIUrl":"10.1080/01478885.2024.2391683","url":null,"abstract":"The discovery of biomarkers, essential for successful drug development, is often hindered by the limited availability of tissue samples, typically obtained through core needle biopsies. Standard 'omics platforms can consume significant amounts of tissue, forcing scientist to trade off spatial context for high-plex assays, such as genome-wide assays. While bulk gene expression approaches and standard single-cell transcriptomics have been valuable in defining various molecular and cellular mechanisms, they do not retain spatial context. As such, they have limited power in resolving tissue heterogeneity and cell-cell interactions. Current spatial transcriptomics platforms offer limited transcriptome coverage and have low throughput, restricting the number of samples that can be analyzed daily or even weekly. While the Digital Spatial Profiling (DSP) method does not provide single-cell resolution, it presents a significant advancement by enabling scalable whole transcriptome and ultrahigh-plex protein analysis from distinct tissue compartments and structures using a single tissue slide. These capabilities overcome significant constraints in biomarker analysis in solid tissue specimens. These advancements in tissue profiling play a crucial role in deepening our understanding of disease biology and in identifying potential therapeutic targets and biomarkers. To enhance the use of spatial biology tools in drug discovery and development, the DSP Scientific Consortium has created best practices guidelines. These guidelines, built on digital spatial profiling data and expertise, offer a practical framework for designing spatial studies and using current and future spatial biology platforms. The aim is to improve tissue analysis in all research areas supporting drug discovery and development.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"7-26"},"PeriodicalIF":0.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing tissue adherence on glass slides using polyurethane glue: a new slide preparation method. 利用聚氨酯胶优化组织粘附在玻片上：一种新的玻片制备方法。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-17 DOI: 10.1080/01478885.2024.2440679

Jeffery T Williams, Tracy A Goodpaster, Miki Haraguchi

引用次数: 0

Multiple levels of Gomori methenamine silver (GMS) stains do not improve diagnostic yield in esophageal biopsies. 高水平的戈莫里甲基苯丙胺银（GMS）染色不能提高食管活检的诊断率。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-12 DOI: 10.1080/01478885.2024.2437587

Michael Occidental, Andrew Dunn, Aaron R Huber

引用次数: 0

Optimizing immunofluorescent staining of H vessels within an irradiated fracture callus in paraffin-embedded tissue samples. 优化石蜡包埋组织样本中辐照骨折茧内 H 血管的免疫荧光染色。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-01 Epub Date: 2024-07-03 DOI: 10.1080/01478885.2024.2371060

Nathan T Sheppard, Melissa C Daniel, Noah S Nelson, Alexis Donneys, Steven R Buchman

{"title":"Optimizing immunofluorescent staining of H vessels within an irradiated fracture callus in paraffin-embedded tissue samples.","authors":"Nathan T Sheppard, Melissa C Daniel, Noah S Nelson, Alexis Donneys, Steven R Buchman","doi":"10.1080/01478885.2024.2371060","DOIUrl":"10.1080/01478885.2024.2371060","url":null,"abstract":"H vessels are an essential link in angiogenic-osteogenic coupling and orchestrate the process of bone healing. H vessels are critically deficient in the setting of radiation-induced fractures, which have been reported to occur in up to 25% of patients undergoing radiotherapy. By increasing H-vessel proliferation, Deferoxamine (DFO) revitalizes the physiologic response to skeletal injury and accelerates irradiated fracture repair. H-vessel quantification is therefore an important outcome measure in histologic analysis of bone healing. However, an optimized protocol for staining H vessels in formalin-fixed paraffin-embedded (FFPE) tissue sections has not been reported. With this protocol, we describe a method of staining FFPE bone samples with minimal background fluorescence and high signal-to-noise ratio. We examined mandibular specimens in a rat model of bone healing from a range of fracture conditions, including healthy bone (Fx), irradiated bone (XFx), and irradiated bone with DFO treatment (XFx-DFO). Quantitative analysis revealed a significant increase of H vessels in the XFxDFO group compared to both the Fx and XFx groups. By optimizing immunofluorescent staining of H vessels in FFPE samples across a range of fracture conditions, we offer investigators an efficacious means of producing reliable imaging for quantitative analysis of H vessels in an irradiated fracture callus.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"173-179"},"PeriodicalIF":0.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of in vivo bio-integration of three hyaluronic acid-based fillers for 26 weeks: a histological study. 三种透明质酸类填充剂 26 周体内生物整合比较分析：组织学研究。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-01 Epub Date: 2024-07-18 DOI: 10.1080/01478885.2024.2369967

Baoji Song, Qiqi Chen

引用次数: 0