Journal of Histotechnology最新文献_第2页

Novel liquid immunocytochemistry with machine learning analysis for bladder cancer detection. 新型液体免疫细胞化学与机器学习分析用于膀胱癌检测。

IF 1.8 4区生物学

Journal of Histotechnology Pub Date : 2025-08-15 DOI: 10.1080/01478885.2025.2546655

Ankush U Patel, Samir Atiya, Yi Song, Wenjiang Chu, Anil V Parwani

{"title":"Novel liquid immunocytochemistry with machine learning analysis for bladder cancer detection.","authors":"Ankush U Patel, Samir Atiya, Yi Song, Wenjiang Chu, Anil V Parwani","doi":"10.1080/01478885.2025.2546655","DOIUrl":"https://doi.org/10.1080/01478885.2025.2546655","url":null,"abstract":"Bladder cancer diagnosis is challenged by invasive monitoring and workflow inefficiencies impacting diagnostic reliability. This prospective study enrolled 150 patients (January 2020-December 2022) and evaluated a novel liquid-based immunocytochemistry platform, coupled with integrated machine learning, for detecting urothelial carcinoma in voided urine. All 150 cytology slides met the adequacy threshold of ≥2,644 urothelial cells and showed preserved cytomorphology. Eight cases of papillary urothelial neoplasm of low malignant potential (PUNLMP) were set aside a-priori, yielding an analytic cohort of 142 patients (115 urothelial-carcinoma, 27 benign) for performance analysis. hTERT (sensitivity 92.2%, specificity 66.7%), GATA-3 (67.0%, 88.9%), and CK17 (89.6%, 66.7%). In multi-marker analysis, sensitivity reached 100% (95% CI 96.8-100) when any marker was positive, whereas specificity reached 100% (95% CI 87.3-100) when all three markers were positive. The workflow-optimized platform standardizes specimen preparation and multi-marker interpretation, offering a robust foundation for urine-based bladder-cancer diagnostics. Larger, multi-center validation studies are warranted to refine specificity estimates and facilitate laboratory integration. This study demonstrates that addressing fundamental workflow challenges in bladder cancer diagnostics before implementing artificial intelligence creates more effective diagnostic tools. By prioritizing specimen integrity and standardization through a novel liquid immunocytochemistry platform, exceptional diagnostic performance was achieved with 100% sensitivity and specificity under defined marker parameters across various cancer stages. This workflow-first approach to integrating machine learning with advanced biomarker analysis offers a model for developing clinically practical diagnostic innovations that can reduce reliance on invasive monitoring procedures while improving detection accuracy.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-9"},"PeriodicalIF":1.8,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Creating synergy between established histology methods and newer spatial analysis modalities using fresh frozen tissues. 在已建立的组织学方法和使用新鲜冷冻组织的较新的空间分析模式之间创造协同作用。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-07-23 DOI: 10.1080/01478885.2025.2530258

Emily R Sekera, Anoop Murthy Kavirayani, Pam Johnson, Heather Sheppard, Roman Kiselev, John J Bowling

{"title":"Creating synergy between established histology methods and newer spatial analysis modalities using fresh frozen tissues.","authors":"Emily R Sekera, Anoop Murthy Kavirayani, Pam Johnson, Heather Sheppard, Roman Kiselev, John J Bowling","doi":"10.1080/01478885.2025.2530258","DOIUrl":"https://doi.org/10.1080/01478885.2025.2530258","url":null,"abstract":"Formalin-fixed paraffin embedded tissues are a mainstay in contemporary histology and pathology practice and represent the most readily available and widely used sample format that has guided early studies and set standards for a new field of spatial biology. However, fixed tissues are not always suitable for all molecular applications and detection of certain molecular targets can be suppressed by these practices. There is a greater diversity of analysis techniques that are commercially available to researchers today, and pathologists and core services are now engaging with groups that prefer fresh-frozen samples more than ever before. Some of these analysis techniques can be characterized as 'label-free' and access unique classes of molecular targets in situ. Our team has developed tools and procedures to facilitate a synergy between the realm of label-free spatial biology and the histology laboratory for biomedical discovery programs. Herein, we discuss the development of 3D printed cassette holders for use in a variety of commercially available cryostats. This apparatus was tested using fresh frozen tissue slices embedded in hydroxypropyl methylcellulose polyvinylpyrrolidone for subsequent analyses. Based on our tests, we propose this protocol as an option to better preserve sample history during the lifetime of a specimen and curtail the decomposition of any lower abundance endogenous chemical moieties for spatial 'omics' analysis using mass spectrometry.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":0.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathological analysis of Artemia franciscana nauplii under different forms of mercury stress. 不同形式汞胁迫下金翅蒿的组织病理学分析。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-07-02 DOI: 10.1080/01478885.2025.2523622

Yanlin Wei, Wei Liu, Fengling Zhang, Yang Chen, Dongxin Wei, Hangyu Lin, Tao He

{"title":"Histopathological analysis of Artemia franciscana nauplii under different forms of mercury stress.","authors":"Yanlin Wei, Wei Liu, Fengling Zhang, Yang Chen, Dongxin Wei, Hangyu Lin, Tao He","doi":"10.1080/01478885.2025.2523622","DOIUrl":"https://doi.org/10.1080/01478885.2025.2523622","url":null,"abstract":"Inorganic mercury (Hg) and methylmercury (MeHg) have emerged as global pollutants owing to their long-term environmental stability and bioaccumulation. These heavy metals enter aquatic systems via industrial emissions, coal combustion, and natural processes, posing a serious threat to ecosystems and human health. This study assesses the impact of Hg2+ and MeHg on the growth and development of brine shrimp (Artemia franciscana) nauplii by analyzing the histopathological effects on their tissues. Brine shrimp nauplii from the Bohai Bay in China were selected as the study subjects and exposed to 1 μmol/L solutions of HgCl₂ and MeHgCl. Tissue sections were continuously taken at different immersion times. After staining with the standard hematoxylin-eosin (HE) method, the tissue morphology of brine shrimp nauplii under different forms of Hg stress was observed under a light microscope. The results showed that MeHg exhibited significantly greater toxicity to brine shrimp nauplii than Hg2+. Under the same exposure time, the MeHg group exhibited more pronounced epithelial cell damage, nuclear material disorder, and nucleoplasm diffusion outside the nucleus than the HgCl₂ group. This finding provides an important theoretical support for further research into the toxicological mechanisms of MeHg and Hg2+, and highlights that the toxic effects of methylmercury on aquatic organisms.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":0.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automatic cell classification and quantification with machine learning in immunohistochemistry images. 免疫组织化学图像中的机器学习自动细胞分类和定量。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-07-01 DOI: 10.1080/01478885.2025.2523618

Pikting Cheung, Wei Zhang, Muhammad Shehzad Khan, Irfan Ahmed, Yuanchao Liu, Fraser Hill, Xinyue Li, Condon Lau

{"title":"Automatic cell classification and quantification with machine learning in immunohistochemistry images.","authors":"Pikting Cheung, Wei Zhang, Muhammad Shehzad Khan, Irfan Ahmed, Yuanchao Liu, Fraser Hill, Xinyue Li, Condon Lau","doi":"10.1080/01478885.2025.2523618","DOIUrl":"https://doi.org/10.1080/01478885.2025.2523618","url":null,"abstract":"The incidence of lymphoma, a cancer that affects both humans and animals, has witnessed a significant increase. In response, immunohistochemistry (IHC) has become an essential tool for its classification. This prompted us to develop an innovative mathematical methodology for the precise quantification of immunopositive and immunonegative cells, along with their spatial analysis, in CD3-stained lymphoma IHC images. Our approach involves integrating an algorithm based on a mathematical color model for cell differentiation, employing the distinctive morphological erosion, algorithmic transformations, and customized histogram equalization to enhance features. Refined local thresholding enhances classification precision. Additionally, a customized circular Hough transform quantifies cell counts and assesses their spatial data. The algorithms accurately enumerate cell types, reducing human intervention and providing total numbers and spatial information on detected cells within tissue specimens. Evaluation of IHC image samples revealed an overall accuracy of 93.98% for automatic cell counts. The automatic counts and location information were cross-validated by three pathology specialists, highlighting the effectiveness and reliability of our automated approach. Our innovative framework enhances lymphoma cell counting accuracy in IHC images by combining physics-based color understanding with machine learning, thereby improving diagnosis and reducing the risks of human error.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-18"},"PeriodicalIF":0.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic yield of stains for infectious organisms in esophageal or gastroesophageal junction biopsies with esophagitis. 食管炎食管或胃食管交界处活检中感染性生物体染色的诊断率。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-06-01 Epub Date: 2024-10-14 DOI: 10.1080/01478885.2024.2415154

Aaron R Huber, Christa L Whitney-Miller

引用次数: 0

AI-Augmented authorship: revolutionizing histopathology publishing in the generative AI era. 人工智能增强的作者身份：在生成式人工智能时代革新组织病理学出版。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-06-01 Epub Date: 2025-05-19 DOI: 10.1080/01478885.2025.2505294

Qiangqiang Gu, Nianyi Li, Nickolas G Littlefield, Fengyi Gao, Monika Lamba Saini, Yongfu Wang, Bradley J Wheeler, T Rinda Soong, Rong Xia, Ahmad P Tafti

引用次数: 0

The impact of gliomas on the normal brain microenvironment: a pilot study. 胶质瘤对正常大脑微环境的影响：一项试点研究。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-06-01 Epub Date: 2024-10-01 DOI: 10.1080/01478885.2024.2408505

Nicole Riha, Jacen S Moore, Sheila Criswell

{"title":"The impact of gliomas on the normal brain microenvironment: a pilot study.","authors":"Nicole Riha, Jacen S Moore, Sheila Criswell","doi":"10.1080/01478885.2024.2408505","DOIUrl":"10.1080/01478885.2024.2408505","url":null,"abstract":"Gliomas are malignant tumors of neuronal support cells within the central nervous system (CNS) and are characterized by poor overall prognoses and limited treatment options due to their infiltrative growth patterns. The neural tumor microenvironment, composed of benign neurons, neuroglia, endothelial cells, and intravascular white blood cells, is a target-rich site for potential chemotherapeutic agents. This study assessed cell proliferation rates, white blood cell components, and a limited number of nuclear, cytoplasmic, and membrane markers using immunohistochemistry (IHC) assays on formalin-fixed and paraffin-embedded benign and glial tumor tissue samples from the CNS. It was observed that glioma tissues had increased rates of glial cell proliferation and significant increases in the number of observed T-lymphocytes and granulocytes but decreased expression of markers Somatostatin receptor 2 (SSTR2), L1 cell adhesion molecule (L1CAM), and GATA binding protein 3 (GATA3) when compared to benign tissue samples. Understanding the lack of protein expression and population expansion potential of the glioma microenvironment in greater detail could help identify valuable therapeutic target combinations for future treatments.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"93-102"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-magnitude high-frequency vibration ameliorates high glucose-induced endothelial injury by restoring mitochondrial function via AMPK/mTOR pathway. 低幅高频振动通过 AMPK/mTOR 途径恢复线粒体功能，从而改善高血糖诱导的内皮损伤。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-06-01 Epub Date: 2024-11-20 DOI: 10.1080/01478885.2024.2429855

Xidan Zhang, Jiyu Sun, Xiting Zhu, Zhenghao Yang, Zhuoli Zhu, Min Zhou, Chen Li, Haiyang Yu, Xueqi Gan

{"title":"Low-magnitude high-frequency vibration ameliorates high glucose-induced endothelial injury by restoring mitochondrial function via AMPK/mTOR pathway.","authors":"Xidan Zhang, Jiyu Sun, Xiting Zhu, Zhenghao Yang, Zhuoli Zhu, Min Zhou, Chen Li, Haiyang Yu, Xueqi Gan","doi":"10.1080/01478885.2024.2429855","DOIUrl":"10.1080/01478885.2024.2429855","url":null,"abstract":"High glucose-induced dysfunction of endothelial cells is a critical and initiating factor in the genesis of diabetic vascular complications. Low-magnitude high-frequency vibration (LMHFV) is a non-invasive biophysical intervention. It has been reported that it exhibits protective effects on high glucose-induced osteoblast dysfunction, but little was known on diabetic vascular complications. In this work, we aim to clarify the role of LMHFV on high glucose-induced endothelial dysfunction and hypothesized that the protective effects functioned through adenosine monophosphate-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway. We cultured primary murine aortic endothelial cells (MAECs) in normal or HG medium, respectively, before exposing to LMHFV. The tube formation, paracellular permeability assay, and aortic ring sprouting assay showed that the high glucose injured-function of MAECs was improved after LMHFV treatment. The intracellular ROS generation analysis, mitochondrial complex I activities measurement, ATP measurement and mitochondrial membrane potential (MMP), and mitochondrial ROS generation analysis of MAECs indicated that mitochondrial function was restored by LMHFV loading in a high glucose environment. Mechanically, western blot assays showed that AMPK phosphorylation was promoted and mTOR was inhibited in LMHFV-induced endothelial function restoration. After the administration of the AMPK inhibitor, Compound C, these protective effects resulting from LMHFV are reversed. These findings suggest that LMHFV plays a significant role in protecting endothelial cells' function and mitochondrial function in high glucose-induced injured MAECs via AMPK/mTOR signalling.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"82-92"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptation of the HistoEnder, an open-source 3D printer for automated transmission electron microscopy grid staining. 对用于自动透射电子显微镜网格染色的开源三维打印机 HistoEnder 进行改装。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-06-01 Epub Date: 2024-09-23 DOI: 10.1080/01478885.2024.2403872

Philip Seifert

引用次数: 0

Histogel-based techniques for embedding organoids in paraffin blocks enable high throughput downstream histopathological analyses. 基于组织凝胶的技术可将有机体包埋在石蜡块中，从而实现高通量的下游组织病理学分析。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-03-01 Epub Date: 2024-09-05 DOI: 10.1080/01478885.2024.2398381

Charles Havnar, Loryn Holokai, Ryan Ichikawa, Wennie Chen, Alexis Scherl, Eliah R Shamir

{"title":"Histogel-based techniques for embedding organoids in paraffin blocks enable high throughput downstream histopathological analyses.","authors":"Charles Havnar, Loryn Holokai, Ryan Ichikawa, Wennie Chen, Alexis Scherl, Eliah R Shamir","doi":"10.1080/01478885.2024.2398381","DOIUrl":"10.1080/01478885.2024.2398381","url":null,"abstract":"Organoids are in vitro tissue models derived from human or animal primary tissues or stem cells that allow for studying three-dimensional (3D) tissue biology, toxicity testing, biomarker evaluation, and assessment of compound efficacy, supplementing or potentially minimizing use of animal models. Organoids are typically cultured in a 3D format within an extracellular matrix and, at the end of an experiment, can be further processed for various cellular or molecular readouts. Analysis often relies on whole mount immunolabeling for markers of interest, which consumes the entire sample/well, thereby limiting sample availability for downstream assays. In addition, 3D cultures become more friable after fixation and are susceptible to sample loss during washing steps. In contrast, by fixing and processing organoids to a paraffin block, dozens or hundreds of unstained slides can be generated, enabling robust characterization via multiple assays, including histologic evaluation and (immuno)histochemical stains, thus maximizing the yield of these time- and labor-intensive cultures. Here we describe three methods to process 3D Matrigel cultures into paraffin blocks using Histogel as an embedding agent. The three techniques all yield high-quality sections but vary in complexity of implementation at different steps, and their application for different use cases is discussed.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"46-57"},"PeriodicalIF":0.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0