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Journal of Histotechnology最新文献

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Comparative analysis of in vivo bio-integration of three hyaluronic acid-based fillers for 26 weeks: a histological study. 三种透明质酸类填充剂 26 周体内生物整合比较分析：组织学研究。
IF 0.6 4区 生物学
Journal of Histotechnology Pub Date : 2024-07-18 DOI: 10.1080/01478885.2024.2369967
Baoji Song, Qiqi Chen
{"title":"Comparative analysis of <i>in vivo</i> bio-integration of three hyaluronic acid-based fillers for 26 weeks: a histological study.","authors":"Baoji Song, Qiqi Chen","doi":"10.1080/01478885.2024.2369967","DOIUrl":"https://doi.org/10.1080/01478885.2024.2369967","url":null,"abstract":"<p><p>Stabilized hyaluronic acid (HA), produced through diverse cross-linking technology and formulated as an injectable gel, has found widespread utilization in aesthetic industry. Cross-linked HA essentially constitutes a gel particle composition formed by numerous viscoelastic particulates. Various product formulations yield HA gels with distinct properties, including particle size, viscoelasticity, and interaction forces between particles. While previous studies have primarily concentrated on the biological safety and macroscopic expression of fillers, limited research exists on the internal mechanisms governing their macro-performance. This study selected three common dermal fillers for analysis, establishing an animal model to assess their <i>in vivo</i> interaction with surrounding tissues and explore their internal mechanisms. The findings revealed that particle size plays a crucial role in tissue integration.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141633686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimizing immunofluorescent staining of H vessels within an irradiated fracture callus in paraffin-embedded tissue samples. 优化石蜡包埋组织样本中辐照骨折茧内 H 血管的免疫荧光染色。
IF 0.6 4区 生物学
Journal of Histotechnology Pub Date : 2024-07-03 DOI: 10.1080/01478885.2024.2371060
Nathan T Sheppard, Melissa C Daniel, Noah S Nelson, Alexis Donneys, Steven R Buchman
{"title":"Optimizing immunofluorescent staining of H vessels within an irradiated fracture callus in paraffin-embedded tissue samples.","authors":"Nathan T Sheppard, Melissa C Daniel, Noah S Nelson, Alexis Donneys, Steven R Buchman","doi":"10.1080/01478885.2024.2371060","DOIUrl":"https://doi.org/10.1080/01478885.2024.2371060","url":null,"abstract":"<p><p>H vessels are an essential link in angiogenic-osteogenic coupling and orchestrate the process of bone healing. H vessels are critically deficient in the setting of radiation-induced fractures, which have been reported to occur in up to 25% of patients undergoing radiotherapy. By increasing H-vessel proliferation, Deferoxamine (DFO) revitalizes the physiologic response to skeletal injury and accelerates irradiated fracture repair. H-vessel quantification is therefore an important outcome measure in histologic analysis of bone healing. However, an optimized protocol for staining H vessels in formalin-fixed paraffin-embedded (FFPE) tissue sections has not been reported. With this protocol, we describe a method of staining FFPE bone samples with minimal background fluorescence and high signal-to-noise ratio. We examined mandibular specimens in a rat model of bone healing from a range of fracture conditions, including healthy bone (Fx), irradiated bone (XFx), and irradiated bone with DFO treatment (XFx-DFO). Quantitative analysis revealed a significant increase of H vessels in the XFxDFO group compared to both the Fx and XFx groups. By optimizing immunofluorescent staining of H vessels in FFPE samples across a range of fracture conditions, we offer investigators an efficacious means of producing reliable imaging for quantitative analysis of H vessels in an irradiated fracture callus.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increase of KLK7, cytokeratin 5/6, and elafin expression in head and neck squamous cell carcinoma compared with lung squamous cell carcinoma. 与肺鳞癌相比，头颈部鳞癌中 KLK7、细胞角蛋白 5/6 和 elafin 的表达增加。
IF 1.1 4区 生物学
Journal of Histotechnology Pub Date : 2024-06-01 Epub Date: 2024-01-08 DOI: 10.1080/01478885.2023.2301123
Angelin Shanmugam, JoAnna Rudasill, Sheila Criswell
{"title":"Increase of KLK7, cytokeratin 5/6, and elafin expression in head and neck squamous cell carcinoma compared with lung squamous cell carcinoma.","authors":"Angelin Shanmugam, JoAnna Rudasill, Sheila Criswell","doi":"10.1080/01478885.2023.2301123","DOIUrl":"10.1080/01478885.2023.2301123","url":null,"abstract":"<p><p>Squamous cell carcinoma is the most common primary tumor in the head and neck epithelium and is the second most common primary tumor type in the lung. Although morphologically indistinguishable from each other with hematoxylin and eosin stain on histology, the tumors have different protein expression profiles. Using 24 formalin-fixed paraffin embedded squamous cell carcinomas of the lung and 24 squamous cell carcinomas in the head and neck, protein expression for cytokeratin 5/6, kallikrein 7, and elafin was evaluated by immunohistochemistry. All three proteins were found to evidence higher expression in head and neck squamous cell carcinoma as compared with that of squamous cell carcinoma of the lung. The differences in expression may help clinical differentiation between primary tumors of the lung from metastatic tumors to the lung from the oral/laryngeal cavities.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139377797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Runx1 alleviates osteoarthritis progression in aging mice. Runx1缓解衰老小鼠骨关节炎的进展。
IF 1.1 4区 生物学
Journal of Histotechnology Pub Date : 2024-06-01 Epub Date: 2023-11-15 DOI: 10.1080/01478885.2023.2281790
Haoran Chen, Caixia Pi, Mingyang Chen, Xinmei Du, Yujia Cui, Demao Zhang, Qiang Guo, Jing Xie, Xuedong Zhou
{"title":"Runx1 alleviates osteoarthritis progression in aging mice.","authors":"Haoran Chen, Caixia Pi, Mingyang Chen, Xinmei Du, Yujia Cui, Demao Zhang, Qiang Guo, Jing Xie, Xuedong Zhou","doi":"10.1080/01478885.2023.2281790","DOIUrl":"10.1080/01478885.2023.2281790","url":null,"abstract":"<p><p>With rates growing quickly with age, osteoarthritis (OA) is the most common cause of chronic disability in aging persons. The discomfort and reduced motion associated with osteoarthritis have a significant impact on quality of life, and there is no known solution. Runt-related transcription factor 1(Runx1) has been shown to play a protective role in the development of osteoarthritis by promoting chondrogenesis. We had created models of ageing mice with osteoarthritis by anterior cruciate ligament transection (ACLT) and analyzed the effects of intra-articular injection of adeno-associated virus/Runx1 (AAV/Runx1) on the models. The results showed that the AAV/Runx1-group maintained better articular cartilage integrity and retained more proteoglycan than the OA group after injection of AAV-Runx1. The markers related to pathological changes in cartilage were downregulated, while the markers related to physiological changes in cartilage were upregulated. This suggests that Runx1 may impede OA progression on the knee joint of ageing mice, potentially playing a protective role in OA and becoming a probable treatment target for osteoarthritis among ageing patients in the future.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107591455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
GW1929 (an agonist of PPARγ) inhibits excessive production of reactive oxygen species in cisplatin-stimulated renal tubular epithelial cells, hampers cell apoptosis, and ameliorates renal injury. GW1929(一种PPARγ激动剂)抑制顺铂刺激的肾小管上皮细胞中活性氧的过量产生，阻碍细胞凋亡，并改善肾损伤。
IF 1.1 4区 生物学
Journal of Histotechnology Pub Date : 2024-06-01 Epub Date: 2023-11-29 DOI: 10.1080/01478885.2023.2286692
Yong He, Caihong Hu, Xin Zhang
{"title":"GW1929 (an agonist of PPARγ) inhibits excessive production of reactive oxygen species in cisplatin-stimulated renal tubular epithelial cells, hampers cell apoptosis, and ameliorates renal injury.","authors":"Yong He, Caihong Hu, Xin Zhang","doi":"10.1080/01478885.2023.2286692","DOIUrl":"10.1080/01478885.2023.2286692","url":null,"abstract":"<p><p>Cisplatin-induced nephrotoxicity has long been explored for development of preventative and therapeutic drugs. The current investigation focused on the renal protective effect of GW1929, an agonist for peroxisome proliferator-activated receptors gamma (PPARγ), on cisplatin-induced kidney injury. HK2 cells treated with 20 μM cisplatin and C57BL/6 mice injected with 20 mg/kg cisplatin were used as the cell model and animal model for acute kidney injury. HK2 cell viability after cisplatin or GW1929 (0-80 μM) treatment was tested using methyl thiazolyl tetrazolium assays. Flow cytometry analysis and TUNEL assays were used to measure cell apoptosis. Intracellular reactive oxygen species (ROS) level was measured through fluorescence intensities. Levels of blood urea nitrogen (BUN) and serum creatinine (SCr) were measured to evaluate the renal function of mice. For renal morphology observation and cell apoptosis assessment <i>in vivo</i>, hematoxylin-eosin staining and TUNEL assays were conducted. The concentrations of oxidative stress markers in renal samples were measured using colorimetric tests. It was found that GW1929 dose-dependently enhanced protein levels of PPARγ, PGC-1α and TFEB in HK2 cells. Meanwhile, intracellular ROS overproduction, the decrease in cell viability and excessive cell apoptosis mediated by cisplatin were reversed by GW1929. For <i>in vivo</i> experiments, GW1929 notably attenuated cisplatin-stimulated nephrotoxicity and oxidative stress while reducing BUN and Scr levels in cisplatin-challenged model mice. Moreover, GW1929 significantly dampened renal cell apoptosis <i>in vivo</i>. GW1929 mitigates renal tubular epithelial cell injury and renal damage by inhibiting oxidative stress and renal cell apoptosis.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138451607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A challenging case of sclerosing polycystic adenoma of the parotid gland: a case report and brief review of the latest updates. 一例具有挑战性的腮腺硬化性多囊腺瘤：病例报告和最新进展简评。
IF 1.1 4区 生物学
Journal of Histotechnology Pub Date : 2024-06-01 Epub Date: 2024-01-15 DOI: 10.1080/01478885.2023.2299911
Radwa Rashad, Abdelrahman Barakat
{"title":"A challenging case of sclerosing polycystic adenoma of the parotid gland: a case report and brief review of the latest updates.","authors":"Radwa Rashad, Abdelrahman Barakat","doi":"10.1080/01478885.2023.2299911","DOIUrl":"10.1080/01478885.2023.2299911","url":null,"abstract":"<p><p>Sclerosing polycystic adenoma (SPA) is a rare neoplastic salivary gland lesion with only about 100 cases reported worldwide so far. The lesion is confused with several malignant and other benign tumors such as apocrine intraductal carcinoma (IC), salivary duct carcinoma (SDC), chronic sclerosing sialadenitis, polycystic dysgenetic disease (PDD), pleomorphic adenoma (PA), acinic cell carcinoma (ACC), and mucoepidermoid carcinoma (MEC). We present a case of SPA for a 23-year-old male patient presenting with a slowly growing parotid mass. Fine needle aspiration (FNA) followed by total excision of the tumor was performed and the picture was consistent with SPA. We discuss the findings of the case and briefly review the literature on SPA.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139466741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Navigating the post-pandemic terrain: lessons in resilience and adaptation. 在大流行后的环境中航行：抗灾和适应方面的经验教训。
IF 1.1 4区 生物学
Journal of Histotechnology Pub Date : 2024-04-26 DOI: 10.1080/01478885.2024.2345443
Chongbei Zhao, Luis A Chiriboga, Elizabeth A Chlipala
{"title":"Navigating the post-pandemic terrain: lessons in resilience and adaptation.","authors":"Chongbei Zhao, Luis A Chiriboga, Elizabeth A Chlipala","doi":"10.1080/01478885.2024.2345443","DOIUrl":"https://doi.org/10.1080/01478885.2024.2345443","url":null,"abstract":"","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140652580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Using machine learning for chemical-free histological tissue staining 利用机器学习进行无化学物质组织染色
IF 1.1 4区 生物学
Journal of Histotechnology Pub Date : 2024-04-22 DOI: 10.1080/01478885.2024.2338585
Julie A. Renner, Patrick C. Riley
{"title":"Using machine learning for chemical-free histological tissue staining","authors":"Julie A. Renner, Patrick C. Riley","doi":"10.1080/01478885.2024.2338585","DOIUrl":"https://doi.org/10.1080/01478885.2024.2338585","url":null,"abstract":"Hematoxylin and eosin staining can be hazardous, expensive, and prone to error and variability. To circumvent these issues, artificial intelligence/machine learning models such as generative advers...","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140635759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular surgical pathology, 2nd edition 分子外科病理学，第 2 版
IF 1.1 4区 生物学
Journal of Histotechnology Pub Date : 2024-04-16 DOI: 10.1080/01478885.2024.2343180
Stephen K. Lau
{"title":"Molecular surgical pathology, 2nd edition","authors":"Stephen K. Lau","doi":"10.1080/01478885.2024.2343180","DOIUrl":"https://doi.org/10.1080/01478885.2024.2343180","url":null,"abstract":"Published in Journal of Histotechnology (Ahead of Print, 2024)","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140573699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A rapid and simple procedure for the whole-mount bone staining of small fish 小鱼整块骨染色的快速简便程序
IF 1.1 4区 生物学
Journal of Histotechnology Pub Date : 2024-04-12 DOI: 10.1080/01478885.2024.2339452
Narawadee Yeesan, Chanyut Sudtongkong, Supparat Kong-Oh, Kitipong Angsujinda, Jes Kettratad, Pisit Poolprasert, Atsuo Iida, Piyakorn Boonyoung, Korakot Nganvongpanit, Anan Kenthao, Gen Kaneko, Sinlapachai Senarat
{"title":"A rapid and simple procedure for the whole-mount bone staining of small fish","authors":"Narawadee Yeesan, Chanyut Sudtongkong, Supparat Kong-Oh, Kitipong Angsujinda, Jes Kettratad, Pisit Poolprasert, Atsuo Iida, Piyakorn Boonyoung, Korakot Nganvongpanit, Anan Kenthao, Gen Kaneko, Sinlapachai Senarat","doi":"10.1080/01478885.2024.2339452","DOIUrl":"https://doi.org/10.1080/01478885.2024.2339452","url":null,"abstract":"The cartilage and bone structure has provided insightful knowledge about evolution and ecology of fish, which is an important component of biological oceanography. However, the whole-body bone stai...","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140573541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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