Journal of Histotechnology最新文献

{"title":"Journal of Histotechnology is actively promoting education on how to write manuscripts.","authors":"Gayle M Callis","doi":"10.1080/01478885.2021.1967578","DOIUrl":"https://doi.org/10.1080/01478885.2021.1967578","url":null,"abstract":"Education about all aspects of histotechnology includes teaching histotechnicians on how to prepare a manuscript for the Journal of Histotechnology. Promoting the journal and encouraging histotechnicians and others on how to write a paper for the Journal of Histotechnology (JOH) has become a recent ongoing project. However, simply writing a paper can be daunting for many particularly if they have never prepared a manuscript before. Several members of the JOH Editorial Board have undertaken the task to teach people about how publication in our journal can be done. This begins with Dr. Sheila Criswell, an experienced author (and histotechnologist) of several JOH publications, who graciously volunteered to present ‘How to Write a Scientific Paper for Publication’ at the 2021 National Society for Histotechnology Symposium/Convention. This is a recorded video presentation which should be available at NSH.org if people cannot attend the S/C. JOH has Instructions for Authors, but these can often be confusing to newcomers and/or even previous authors involved with writing a manuscript. Hence, a presentation in a lecture format should provide a better explanation of how to write a manuscript and is very welcome. Please note that Dr. Criswell and her histotechnology student have a well written publication in this issue titled ‘Hidradentitis suppurativa lesions are associated with decreased collagen, decreased elastin and increased neovascularization,’ and is a fine example of what she will be teaching in her S/C presentation. Further undertakings on various aspects of what is needed in a manuscript will be forthcoming. Dr. Luis Chiriboga is preparing a blog series on performing a literature search. All authors need cited publications to back up or even refute their work. Using a literature search is not confined to authors for their papers, but also to histotechnicians seeking practical information useful in their everyday laboratory work. Topics in JOH are not limited to bench work but so much more as suggested in areas found in the JOH Aims and Scope found on the journal webpage: https://www.tandfonline.com/toc/ yhis20/current. More presentations and/or blogs are being planned including what is expected in performing a peer review of a manuscript and another on creating tables, and figures with photo images and graphs. Another topic could be how to convert a S/C poster presentation to a manuscript – after all the work is done and merely needs to be expanded into a manuscript. Questions are always welcome if someone is not clear about manuscript writing. The articles in this issue deal with a variety of topics in areas of the Aim and Scope of this journal. We are all interested in environmental impacts of toxic chemicals even from other parts of the world. The paper by Mishra and coworkers discusses histological alterations in a fish species from India that are affected by such pollution. Amiri and colleagues present a histological study on the use of top","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"113"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39395092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Test your knowledge.","authors":"Brooke H Dubansky, Dale Telgenhoff","doi":"10.1080/01478885.2021.1962116","DOIUrl":"https://doi.org/10.1080/01478885.2021.1962116","url":null,"abstract":"","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"161"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39395093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study on the immunohistochemical expression of napsin A in oral squamous cell carcinomas, intraepithelial neoplasia, and normal oral mucosa.","authors":"Nicolay Gaydarov,&nbsp;Carla P Martinelli-Kläy,&nbsp;Tommaso Lombardi","doi":"10.1080/01478885.2021.1894391","DOIUrl":"https://doi.org/10.1080/01478885.2021.1894391","url":null,"abstract":"<p><p>Napsin A is an aspartic proteinase expressed in some types of carcinomas, such as lung adenocarcinomas and renal cell carcinomas but rarely in squamous cell carcinomas. No specific studies have been carried out focusing on napsin A antibody expression in oral squamous cell carcinomas (OSCC). The aim of this study was to investigate the reactivity of this antibody in primary OSCC. This retrospective study included 70 OSCC cases of which 31 (44.3%) presented metastasis involvement. Patient data, including age, gender, tumor location, histological grade, regional and distant metastasis, were collected. OSCC edge epithelium with intraepithelial neoplasia and healthy oral mucosa (n = 10) were included in the analysis. Sections of lung adenocarcinomas (n = 2) were used as the positive control and an immunohistochemical assay for napsin A was performed on all cases. Napsin A expression was negative in all 70 cases of OSCC, as well as in the intraepithelial neoplasia adjacent to the carcinoma area and in healthy oral mucosa epithelium. Metastatic neck lymph nodes and distant organs were also negative for napsin A. This study shows that napsin A is consistently not expressed in oral squamous cell carcinoma, or in metastatic sites of primary OSCC, intraepithelial neoplasia, and healthy oral mucosa.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"139-143"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2021.1894391","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38891306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A method of restoration and preservation of mounted anatomical pathology specimens.","authors":"Manisha Kulkarni,&nbsp;Pradeep Vaideeswar","doi":"10.1080/01478885.2021.1884352","DOIUrl":"https://doi.org/10.1080/01478885.2021.1884352","url":null,"abstract":"<p><p>Big hospitals and biology museums often have a sizable collection of valuable archived anatomical pathology specimens, collected over a long period of time. Traditionally, these specimens are suspended by thread tied to a frame of glass rods in a rectangular glass jar. While restoring with preserving solution, specimens often fell from sewn thread or strings. We developed an easy method of remounting such specimens on Perspex sheets with cyanoacrylate adhesive. This technique was tested on five archived specimens and five recent well-fixed specimens, including brain. Specimens could be easily oriented, remounted and remained well adhered to the Perspex sheet. Gross pathologic characteristics could be well illustrated from all sides in the remounted specimen. Cyanoacrylate adhesive technique was useful in permanent preservation of old delicate anatomical pathology specimens, which could not be tied to glass rods again and had fallen out of position in mounting jars. This technique can also be applied for well-fixed recent specimens. It is easy, cost effective, serves as an educational tool and can be applied to all types of specimens.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"144-149"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2021.1884352","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38908229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological changes of the myenteric plexus at different gut segments of human fetuses.","authors":"Subhash Bhukya,&nbsp;Singh S,&nbsp;Sarwar S,&nbsp;Quadri J A,&nbsp;Ahmed A,&nbsp;Neerja Rani,&nbsp;Dheeraj K,&nbsp;Dubey A,&nbsp;Tapas Chandra Nag,&nbsp;Shariff A","doi":"10.1080/01478885.2020.1862604","DOIUrl":"https://doi.org/10.1080/01478885.2020.1862604","url":null,"abstract":"<p><p>The neural crest cell-derived enteric nervous system (ENS) is the intrinsic innervation of the gastrointestinal tract (GIT) which consists of neurons and enteric glia cells in the myenteric ganglia and forming plexus. The ENS consists mainly of submucosal and myenteric plexuses. It has various functions on the GIT, which include control of local blood flow, motility, mucosal transport, secretions, immune modulation as well as endocrine functions and coordinated contractile activity of smooth muscle. The knowledge on the development of the innervations at different segments of the gut in humans from 11 to 26 weeks of gestation (WG) may help in understanding the pathophysiology of various congenital diseases affecting the ENS. The aim of this study is to determine the morphology of the myenteric plexus in the esophagus, ascending colon and sigmoid colon at various weeks of gestation. Tissue samples from 10 naturally terminated fetuses aged 11-26 WG were processed for hematoxylin and eosin staining and immunohistochemistry assay. The neurons, enteric glia, the smooth muscle were visualized using PGP9.5, Vimentin and S-100 antibodies. The number of neurons and enteric glial cells appeared lowest in the esophagus than the ascending and sigmoid colon. The myenteric ganglion was closely apposed to each other, forming a continuous arch along the entire circumference of gut sections of ascending and sigmoid colon but the myenteric ganglia in the esophagus was thinly populated and widely spread in the fetus at 13 WG. As the fetal gastrointestinal tract grew in diameter and length, the myenteric ganglia became discernible.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"150-159"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2020.1862604","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38815669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorative activity of aqueous leaf extract from <i>Madhuca longifolia</i> against diclofenac-administered toxicity on rat stomach and intestine.","authors":"Jerine Peter Simon,&nbsp;Sabina Evan Prince","doi":"10.1080/01478885.2020.1861917","DOIUrl":"https://doi.org/10.1080/01478885.2020.1861917","url":null,"abstract":"<p><p><i>Madhuca longifolia</i>, a tropical tree used as medicine and food, is known to have a beneficial effect against stomach gastric toxicity. <i>Madhuca longifolia</i> is used in treating cough, skin disease and nerve disorders. Diclofenac, a non-steroidal anti-inflammatory drug (NSAID), with overdosage and prolonged use, is known to cause gastric toxicity. Silymarin (SLY), a polyphenolic antioxidant flavonoid, is a derivative of <i>Silybum marianum</i> extracted from milk thistle seeds and fruits, has been widely used in the treatment of gastric ulcer. SLY was used as the standard drug to compare the effects with the <i>Madhuca longifolia</i> aqueous leaf extract treatment. The aim of the current study is to understand the effect of <i>Madhuca longifolia</i> aq. leaf extract on rat stomach and intestine against diclofenac-administered toxicity. Rats (n = 30) were divided into Group I normal control, Group II treated with diclofenac, Group III treated with diclofenac and <i>Madhuca longifolia</i> leaf extract, Group IV treated with diclofenac and silymarin, and Group V was treated with <i>Madhuca longifolia</i> leaf extract alone. After the study duration, rats were euthanized and tissue samples were analyzed for antioxidant, cytokine, protein expression levels and histopathological changes. Diclofenac treated rats had significant (<i>p</i> < 0.05) changes in levels of antioxidants, cytokines, protein expression and pathological changes as compared to rats treated with <i>Madhuca longifolia</i>. This study demonstrated that <i>Madhuca longifolia</i> leaf extract had gastroprotective activity in rats treated with diclofenac.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"114-126"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2020.1861917","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25426022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective role of All <i>Trans</i> Retinoic Acid on B16F10 melanoma cell line metastasis in C57BL/6 mice by enhancing RAR- β protein and homeostasis maintenance.","authors":"V M Berlin Grace,&nbsp;S Saranya,&nbsp;D David Wilson","doi":"10.1080/01478885.2021.1896291","DOIUrl":"https://doi.org/10.1080/01478885.2021.1896291","url":null,"abstract":"<p><p>Lung cancer is the leading cancer according to the World Health Organization (WHO), resulting in highest death rate worldwide due to the high level of metastasis. Hence, the drugs that protect from metastasis either as an adjuvant or a primary therapeutic agent may help to reduce the death rate. In this study, All Trans Retinoic Acid (ATRA) was tested for its action against metastatic lodging of B16F10 melanoma cells in the lung and liver of the C57BL/6 mouse model. Serum, lung and liver were evaluated biochemically for the cancer associated changes. Metastatic cancer development was confirmed by tumor nodule formation and histopathological analysis. RAR-β protein expression was analyzed by immunohistochemistry and histopathology. ATRA treated mice showed a percentage of inhibition on metastatic tumor growth in lung and liver and a corresponding protection against pathological changes in these organs. Cholesterol and γ-Glutamyl Transferase (GGT) levels found in cancer induced mice were reduced in the ATRA treated group. As compared to the normal group, lung tissue from cell line induced cancer control group had less RAR-β protein expression while the ATRA treated group showed enhanced RAR-β protein expression. This indicates that the anti-metastasis effects of ATRA might have shown the induction of RAR-β expression and subsequent molecular signaling pathways to regulate the homeostasis of biochemical changes. This study demonstrated the capability of ATRA to prevent the establishment of metastasis by the melanoma cell line into the lung and liver of experimental mice.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"127-138"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2021.1896291","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38947608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The spectrum of neuroendocrine neoplasia: a practical approach to diagnosis, classification and therapy","authors":"S. Lau","doi":"10.1080/01478885.2021.1962611","DOIUrl":"https://doi.org/10.1080/01478885.2021.1962611","url":null,"abstract":"Neuroendocrine neoplasms are ubiquitous, and they involve every organ system in the human body. These are lesions that need to be considered in the differential diagnosis of any wellformed glandular or poorly differentiated solid tumors seen in everyday practice. In recent years there also have been major developments in the elucidation of molecular mechanisms and newly available antibodies for the diagnosis of these tumors. This new tome from Springer nature is timely, and it offers an encyclopedic coverage of this group of diverse tumors. My review is based on the e-book version which is available for purchase and download from www.springer.com website. The book is divided into 19 chapters, and it has 26 authors including the 3 editors, most of them pathologists but also several endocrinologists, radiologists, and oncologists. The first three chapters of the book deal with general background information about neuroendocrine neoplasms including historical aspects such as Goliath suffering from possible gigantism and the Egyptian New Kingdom pharaoh Akhenaten. Chapters 2 and 3 deal with the clinical and radiological diagnosis of neuroendocrine neoplasms respectively, followed by the bulk of the book (Chapters 4–15) which discusses neuroendocrine neoplasm in the different organ systems. The final four chapters deal with special circumstances such as unknown metastatic neuroendocrine carcinomas, inherited syndromes, cytologic diagnosis of neuroendocrine tumors and treatment aspects of these tumors. In the e-book, there are links to all the chapters and the references when available, making for easy navigation. The book is beautifully illustrated with numerous photomicrographs, and the tables and graphs are comprehensive, detailed and easy to read. In general, this is a great up-to-date reference book for neuroendocrine neoplasia, and it is one of the best ones available right now in the market for most anatomic pathology laboratories dealing with this common everyday neoplasm.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 1","pages":"160 - 160"},"PeriodicalIF":1.1,"publicationDate":"2021-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45807063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Grape seed proanthocyanidins improves depression-like behavior by alleviating oxidative stress and NLRP3 activation in the hippocampus of prenatally-stressed female offspring rats.","authors":"Qinru Sun,&nbsp;Ning Jia,&nbsp;Fei Ren,&nbsp;Xin Li","doi":"10.1080/01478885.2020.1861907","DOIUrl":"https://doi.org/10.1080/01478885.2020.1861907","url":null,"abstract":"<p><p>Over several decades, there is a growing evidence, which has shown that prenatal stress (PS) contributes to depression in offspring. Grape seed proanthocyanidins (GSPs), which contain dimers, trimers, oligomers of catechin and epicatechin, are known to possess antidepressant effects. The present study aimed to investigate the mechanism of antidepressant effects of GSPs on female juvenile prenatally stressed offspring rats. The results showed that the female juvenile offspring rats exposed to PS exhibited depression-like behavior manifested as longer immobility time and lesser consumption of sucrose solution. Prenatal stress reduced the number of hippocampal neurons and increased the level of the reactive oxygen species (ROS) in the hippocampus of the female juvenile offspring rats. Furthermore, the expression of PYD domains-containing protein 3 (NLRP3) and its downstream cytokines, Caspase-1, and interleukin-1β (IL-1β), were increased in the hippocampus of the female juvenile offspring rats exposed to PS. Administration of GSPs not only improved depression-like behavior and enhanced the number of hippocampal neurons, but also abated excessive ROS generation and inhibited the activation of the NLRP3-Caspase-1 signaling pathway. Taken together, GSPs counteract PS-induced hippocampal neuron loss and depression-like behavior by alleviating oxidative stress and NLRP3 activation. The present study provides a new insight for GSPs as an effective therapeutic agent for adolescent depression.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 2","pages":"90-98"},"PeriodicalIF":1.1,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2020.1861907","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38805207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in manuscript submissions: an issue combined with international and domestic articles.","authors":"Gayle M Callis","doi":"10.1080/01478885.2021.1923887","DOIUrl":"https://doi.org/10.1080/01478885.2021.1923887","url":null,"abstract":"By the time you read this issue, we will be half-way through 2021, an extraordinary year in so many ways. Noted is a recent science magazine [1] that is a commentary about countries/regions in the Middle East and North Africa as the source of increased manuscript submissions to scientific journals from 1981 to 2019, with only China ‘growing’ by more. The article also discussed other ramifications of these regions that contributed to this increase in these submissions. This submission trend has not gone unnoticed and can be seen in this issue as JOH becomes more recognized internationally. Our readers, who are interested in fish histology and the effects of heavy metal pollution on reproductive organs, should find the paper educational. The Mishra group studied alterations in ovaries caused by heavy metal pollutants from fish that were living in a wetland habitat in India. This study used well-illustrated histological methods, scanning electron microscopy, and elemental x-ray diffractions and pointed out the danger that chemical pollution has on fish reproduction and the threat pollution can have on worldwide aquatic resources. The Amiri group used a rat model for a histological study on the combined effect of topical insulin mixed with an antibacterial ointment and found it was more effective for wound healing than each separate agent. Hopefully, these authors return with another study to tell more of this story per their recommendations for different drug doses and other methods to determine what growth factors contribute to the mechanism of action. Studies on natural products remain very important for the treatment of various diseases and conditions. Grape seed proanthocyanidins (GSP) were used to study depression-like behavior in prenatally stressed juvenile rats. Sun and colleagues had a comprehensive study on GSP treatments for depressionlike behavior that included stress testing, Nissl stains for neuron loss, reactive oxygen species (ROS) measurement, and Western blot analyses. Readers should note how antibodies for Western blot or for immunohistochemistry (IHC) are reported to provide detailed information when purchasing antibodies. It is always a pleasure to see a paper on bone which remains a difficult tissue to work with in the laboratory. Studies using induced osteoarthritis (OA) on murine knee joints are common in the literature. Wang et al. developed an OA model for anterior cruciate ligament surgery to study the alteration of A disintegrin and metalloproteinase with thrombospondin motifs. The authors present detailed methods and materials for surgically induced OA on knees followed by extensive histology with routine and special stains for bone components, TRAP staining for osteoclasts, along with RT-PCR, Elisa, and immunofluorescence assays. This is a good example of a comprehensive study with all the tests and histology done for those interested in OA studies on the murine model. Last but not least is an interesting paper from Cri","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 2","pages":"61"},"PeriodicalIF":1.1,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2021.1923887","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39239644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}