Journal of Histotechnology最新文献

{"title":"Grossing, staging and reporting: an integrated manual of modern surgical pathology","authors":"S. Lau","doi":"10.1080/01478885.2022.2026676","DOIUrl":"https://doi.org/10.1080/01478885.2022.2026676","url":null,"abstract":"","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"45 1","pages":"49 - 49"},"PeriodicalIF":1.1,"publicationDate":"2022-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45681980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of ergot alkaloid exposure during gestation on the microscopic morphology and vasculature of the ovine placenta.","authors":"J L Britt,&nbsp;R R Powell,&nbsp;C McMahan,&nbsp;T F Bruce,&nbsp;S K Duckett","doi":"10.1080/01478885.2021.1902670","DOIUrl":"https://doi.org/10.1080/01478885.2021.1902670","url":null,"abstract":"<p><p>Ergot alkaloids, a class of mycotoxins associated with ergotism, act as agonists on serotonin (5HT) receptors, specifically 5HT2a, which mediate smooth muscle contraction and vasoconstriction. The objective of this study was to examine the impact of ergot alkaloid exposure during mid and late gestation on microscopic placental structure and vascular development. Ewes were fed endophyte-infected tall fescue seed containing ergot alkaloids (E+/E+, 1.77 mg ewe^-1 d^-1) or endophyte-free tall fescue seed (E-/E-, 0 mg ergot alkaloids) during both mid (d 35 to d 85) and late gestation (d 86 to d 133). On d 133 of gestation, a terminal surgery was performed and two placentomes of the type B morphology were collected for microscopic analyses. Amorphous connective tissue regions were larger (<i>p</i> < 0.0001) and more numerous (<i>p</i> = 0.025) in the placentome of ergot alkaloid exposed ewes. Staining showed no difference (<i>p</i> = 0.83) in the number of vessels present, but luminal area of maternal vasculature was 117% greater (<i>p</i> < 0.0001) in ergot alkaloid exposed ewes. Results showed that exposure to ergot alkaloids during gestation slowed maturation of the fetal villi as indicated by greater amorphous connective tissue regions, and altered size and shape of blood vessels to counteract reductions in blood flow caused by vasoconstriction.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 4","pages":"173-181"},"PeriodicalIF":1.1,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2021.1902670","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9422798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atlas of diagnostic and predictive histopathology","authors":"S. Lau","doi":"10.1080/01478885.2021.1996990","DOIUrl":"https://doi.org/10.1080/01478885.2021.1996990","url":null,"abstract":"This is the second edition of a pathology atlas on diagnostic and predictive histopathology, with a stronger emphasis on the former than the latter. The book is divided into 16 chapters with one chapter on basic pathology which also includes many infectious diseases and one chapter on predictive histopathology including breast markers and hematolymphoid markers. The remaining 14 chapters deal with the various organ systems in the body. Each disease entity is usually represented by a low power and a higher power photomicrograph followed by photomicrographs or diagrams of special studies such as immunohistochemistry, FISH and flow cytometry. The book is comprehensive with numerous illustrations, and it covers most disease entities one is likely to encounter in day-to-day practice. The captions are well written and easy to follow. In the next edition of this atlas, the authors may want to include the predictive markers with the organ system instead of having a separate chapter in the middle of the book. They may also want to include some of the staging and classification systems which have been omitted from the book. A reference would also be helpful for those who may want more information on a particular topic. An index of the different disease entities either in the beginning of each chapter or at the end of the book would make for easier search. Overall, this is an excellent illustrated pathology atlas covering many disease entities and serves as a great reference for most pathology laboratories.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 1","pages":"234 - 234"},"PeriodicalIF":1.1,"publicationDate":"2021-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47098210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Journal of Histotechnology is actively promoting education on how to write manuscripts.","authors":"Gayle M Callis","doi":"10.1080/01478885.2021.1967578","DOIUrl":"https://doi.org/10.1080/01478885.2021.1967578","url":null,"abstract":"Education about all aspects of histotechnology includes teaching histotechnicians on how to prepare a manuscript for the Journal of Histotechnology. Promoting the journal and encouraging histotechnicians and others on how to write a paper for the Journal of Histotechnology (JOH) has become a recent ongoing project. However, simply writing a paper can be daunting for many particularly if they have never prepared a manuscript before. Several members of the JOH Editorial Board have undertaken the task to teach people about how publication in our journal can be done. This begins with Dr. Sheila Criswell, an experienced author (and histotechnologist) of several JOH publications, who graciously volunteered to present ‘How to Write a Scientific Paper for Publication’ at the 2021 National Society for Histotechnology Symposium/Convention. This is a recorded video presentation which should be available at NSH.org if people cannot attend the S/C. JOH has Instructions for Authors, but these can often be confusing to newcomers and/or even previous authors involved with writing a manuscript. Hence, a presentation in a lecture format should provide a better explanation of how to write a manuscript and is very welcome. Please note that Dr. Criswell and her histotechnology student have a well written publication in this issue titled ‘Hidradentitis suppurativa lesions are associated with decreased collagen, decreased elastin and increased neovascularization,’ and is a fine example of what she will be teaching in her S/C presentation. Further undertakings on various aspects of what is needed in a manuscript will be forthcoming. Dr. Luis Chiriboga is preparing a blog series on performing a literature search. All authors need cited publications to back up or even refute their work. Using a literature search is not confined to authors for their papers, but also to histotechnicians seeking practical information useful in their everyday laboratory work. Topics in JOH are not limited to bench work but so much more as suggested in areas found in the JOH Aims and Scope found on the journal webpage: https://www.tandfonline.com/toc/ yhis20/current. More presentations and/or blogs are being planned including what is expected in performing a peer review of a manuscript and another on creating tables, and figures with photo images and graphs. Another topic could be how to convert a S/C poster presentation to a manuscript – after all the work is done and merely needs to be expanded into a manuscript. Questions are always welcome if someone is not clear about manuscript writing. The articles in this issue deal with a variety of topics in areas of the Aim and Scope of this journal. We are all interested in environmental impacts of toxic chemicals even from other parts of the world. The paper by Mishra and coworkers discusses histological alterations in a fish species from India that are affected by such pollution. Amiri and colleagues present a histological study on the use of top","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"113"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39395092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Test your knowledge.","authors":"Brooke H Dubansky,&nbsp;Dale Telgenhoff","doi":"10.1080/01478885.2021.1962116","DOIUrl":"https://doi.org/10.1080/01478885.2021.1962116","url":null,"abstract":"","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"161"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39395093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study on the immunohistochemical expression of napsin A in oral squamous cell carcinomas, intraepithelial neoplasia, and normal oral mucosa.","authors":"Nicolay Gaydarov,&nbsp;Carla P Martinelli-Kläy,&nbsp;Tommaso Lombardi","doi":"10.1080/01478885.2021.1894391","DOIUrl":"https://doi.org/10.1080/01478885.2021.1894391","url":null,"abstract":"<p><p>Napsin A is an aspartic proteinase expressed in some types of carcinomas, such as lung adenocarcinomas and renal cell carcinomas but rarely in squamous cell carcinomas. No specific studies have been carried out focusing on napsin A antibody expression in oral squamous cell carcinomas (OSCC). The aim of this study was to investigate the reactivity of this antibody in primary OSCC. This retrospective study included 70 OSCC cases of which 31 (44.3%) presented metastasis involvement. Patient data, including age, gender, tumor location, histological grade, regional and distant metastasis, were collected. OSCC edge epithelium with intraepithelial neoplasia and healthy oral mucosa (n = 10) were included in the analysis. Sections of lung adenocarcinomas (n = 2) were used as the positive control and an immunohistochemical assay for napsin A was performed on all cases. Napsin A expression was negative in all 70 cases of OSCC, as well as in the intraepithelial neoplasia adjacent to the carcinoma area and in healthy oral mucosa epithelium. Metastatic neck lymph nodes and distant organs were also negative for napsin A. This study shows that napsin A is consistently not expressed in oral squamous cell carcinoma, or in metastatic sites of primary OSCC, intraepithelial neoplasia, and healthy oral mucosa.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"139-143"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2021.1894391","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38891306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A method of restoration and preservation of mounted anatomical pathology specimens.","authors":"Manisha Kulkarni,&nbsp;Pradeep Vaideeswar","doi":"10.1080/01478885.2021.1884352","DOIUrl":"https://doi.org/10.1080/01478885.2021.1884352","url":null,"abstract":"<p><p>Big hospitals and biology museums often have a sizable collection of valuable archived anatomical pathology specimens, collected over a long period of time. Traditionally, these specimens are suspended by thread tied to a frame of glass rods in a rectangular glass jar. While restoring with preserving solution, specimens often fell from sewn thread or strings. We developed an easy method of remounting such specimens on Perspex sheets with cyanoacrylate adhesive. This technique was tested on five archived specimens and five recent well-fixed specimens, including brain. Specimens could be easily oriented, remounted and remained well adhered to the Perspex sheet. Gross pathologic characteristics could be well illustrated from all sides in the remounted specimen. Cyanoacrylate adhesive technique was useful in permanent preservation of old delicate anatomical pathology specimens, which could not be tied to glass rods again and had fallen out of position in mounting jars. This technique can also be applied for well-fixed recent specimens. It is easy, cost effective, serves as an educational tool and can be applied to all types of specimens.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"144-149"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2021.1884352","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38908229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological changes of the myenteric plexus at different gut segments of human fetuses.","authors":"Subhash Bhukya,&nbsp;Singh S,&nbsp;Sarwar S,&nbsp;Quadri J A,&nbsp;Ahmed A,&nbsp;Neerja Rani,&nbsp;Dheeraj K,&nbsp;Dubey A,&nbsp;Tapas Chandra Nag,&nbsp;Shariff A","doi":"10.1080/01478885.2020.1862604","DOIUrl":"https://doi.org/10.1080/01478885.2020.1862604","url":null,"abstract":"<p><p>The neural crest cell-derived enteric nervous system (ENS) is the intrinsic innervation of the gastrointestinal tract (GIT) which consists of neurons and enteric glia cells in the myenteric ganglia and forming plexus. The ENS consists mainly of submucosal and myenteric plexuses. It has various functions on the GIT, which include control of local blood flow, motility, mucosal transport, secretions, immune modulation as well as endocrine functions and coordinated contractile activity of smooth muscle. The knowledge on the development of the innervations at different segments of the gut in humans from 11 to 26 weeks of gestation (WG) may help in understanding the pathophysiology of various congenital diseases affecting the ENS. The aim of this study is to determine the morphology of the myenteric plexus in the esophagus, ascending colon and sigmoid colon at various weeks of gestation. Tissue samples from 10 naturally terminated fetuses aged 11-26 WG were processed for hematoxylin and eosin staining and immunohistochemistry assay. The neurons, enteric glia, the smooth muscle were visualized using PGP9.5, Vimentin and S-100 antibodies. The number of neurons and enteric glial cells appeared lowest in the esophagus than the ascending and sigmoid colon. The myenteric ganglion was closely apposed to each other, forming a continuous arch along the entire circumference of gut sections of ascending and sigmoid colon but the myenteric ganglia in the esophagus was thinly populated and widely spread in the fetus at 13 WG. As the fetal gastrointestinal tract grew in diameter and length, the myenteric ganglia became discernible.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"150-159"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2020.1862604","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38815669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorative activity of aqueous leaf extract from <i>Madhuca longifolia</i> against diclofenac-administered toxicity on rat stomach and intestine.","authors":"Jerine Peter Simon,&nbsp;Sabina Evan Prince","doi":"10.1080/01478885.2020.1861917","DOIUrl":"https://doi.org/10.1080/01478885.2020.1861917","url":null,"abstract":"<p><p><i>Madhuca longifolia</i>, a tropical tree used as medicine and food, is known to have a beneficial effect against stomach gastric toxicity. <i>Madhuca longifolia</i> is used in treating cough, skin disease and nerve disorders. Diclofenac, a non-steroidal anti-inflammatory drug (NSAID), with overdosage and prolonged use, is known to cause gastric toxicity. Silymarin (SLY), a polyphenolic antioxidant flavonoid, is a derivative of <i>Silybum marianum</i> extracted from milk thistle seeds and fruits, has been widely used in the treatment of gastric ulcer. SLY was used as the standard drug to compare the effects with the <i>Madhuca longifolia</i> aqueous leaf extract treatment. The aim of the current study is to understand the effect of <i>Madhuca longifolia</i> aq. leaf extract on rat stomach and intestine against diclofenac-administered toxicity. Rats (n = 30) were divided into Group I normal control, Group II treated with diclofenac, Group III treated with diclofenac and <i>Madhuca longifolia</i> leaf extract, Group IV treated with diclofenac and silymarin, and Group V was treated with <i>Madhuca longifolia</i> leaf extract alone. After the study duration, rats were euthanized and tissue samples were analyzed for antioxidant, cytokine, protein expression levels and histopathological changes. Diclofenac treated rats had significant (<i>p</i> < 0.05) changes in levels of antioxidants, cytokines, protein expression and pathological changes as compared to rats treated with <i>Madhuca longifolia</i>. This study demonstrated that <i>Madhuca longifolia</i> leaf extract had gastroprotective activity in rats treated with diclofenac.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"114-126"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2020.1861917","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25426022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective role of All <i>Trans</i> Retinoic Acid on B16F10 melanoma cell line metastasis in C57BL/6 mice by enhancing RAR- β protein and homeostasis maintenance.","authors":"V M Berlin Grace,&nbsp;S Saranya,&nbsp;D David Wilson","doi":"10.1080/01478885.2021.1896291","DOIUrl":"https://doi.org/10.1080/01478885.2021.1896291","url":null,"abstract":"<p><p>Lung cancer is the leading cancer according to the World Health Organization (WHO), resulting in highest death rate worldwide due to the high level of metastasis. Hence, the drugs that protect from metastasis either as an adjuvant or a primary therapeutic agent may help to reduce the death rate. In this study, All Trans Retinoic Acid (ATRA) was tested for its action against metastatic lodging of B16F10 melanoma cells in the lung and liver of the C57BL/6 mouse model. Serum, lung and liver were evaluated biochemically for the cancer associated changes. Metastatic cancer development was confirmed by tumor nodule formation and histopathological analysis. RAR-β protein expression was analyzed by immunohistochemistry and histopathology. ATRA treated mice showed a percentage of inhibition on metastatic tumor growth in lung and liver and a corresponding protection against pathological changes in these organs. Cholesterol and γ-Glutamyl Transferase (GGT) levels found in cancer induced mice were reduced in the ATRA treated group. As compared to the normal group, lung tissue from cell line induced cancer control group had less RAR-β protein expression while the ATRA treated group showed enhanced RAR-β protein expression. This indicates that the anti-metastasis effects of ATRA might have shown the induction of RAR-β expression and subsequent molecular signaling pathways to regulate the homeostasis of biochemical changes. This study demonstrated the capability of ATRA to prevent the establishment of metastasis by the melanoma cell line into the lung and liver of experimental mice.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"44 3","pages":"127-138"},"PeriodicalIF":1.1,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/01478885.2021.1896291","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38947608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}