Journal of Histotechnology最新文献

Diagnostic yield of stains for infectious organisms in esophageal or gastroesophageal junction biopsies with esophagitis. 食管炎食管或胃食管交界处活检中感染性生物体染色的诊断率。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-06-01 Epub Date: 2024-10-14 DOI: 10.1080/01478885.2024.2415154

Aaron R Huber, Christa L Whitney-Miller

引用次数: 0

AI-Augmented authorship: revolutionizing histopathology publishing in the generative AI era. 人工智能增强的作者身份：在生成式人工智能时代革新组织病理学出版。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-06-01 Epub Date: 2025-05-19 DOI: 10.1080/01478885.2025.2505294

Qiangqiang Gu, Nianyi Li, Nickolas G Littlefield, Fengyi Gao, Monika Lamba Saini, Yongfu Wang, Bradley J Wheeler, T Rinda Soong, Rong Xia, Ahmad P Tafti

引用次数: 0

The impact of gliomas on the normal brain microenvironment: a pilot study. 胶质瘤对正常大脑微环境的影响：一项试点研究。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-06-01 Epub Date: 2024-10-01 DOI: 10.1080/01478885.2024.2408505

Nicole Riha, Jacen S Moore, Sheila Criswell

{"title":"The impact of gliomas on the normal brain microenvironment: a pilot study.","authors":"Nicole Riha, Jacen S Moore, Sheila Criswell","doi":"10.1080/01478885.2024.2408505","DOIUrl":"10.1080/01478885.2024.2408505","url":null,"abstract":"Gliomas are malignant tumors of neuronal support cells within the central nervous system (CNS) and are characterized by poor overall prognoses and limited treatment options due to their infiltrative growth patterns. The neural tumor microenvironment, composed of benign neurons, neuroglia, endothelial cells, and intravascular white blood cells, is a target-rich site for potential chemotherapeutic agents. This study assessed cell proliferation rates, white blood cell components, and a limited number of nuclear, cytoplasmic, and membrane markers using immunohistochemistry (IHC) assays on formalin-fixed and paraffin-embedded benign and glial tumor tissue samples from the CNS. It was observed that glioma tissues had increased rates of glial cell proliferation and significant increases in the number of observed T-lymphocytes and granulocytes but decreased expression of markers Somatostatin receptor 2 (SSTR2), L1 cell adhesion molecule (L1CAM), and GATA binding protein 3 (GATA3) when compared to benign tissue samples. Understanding the lack of protein expression and population expansion potential of the glioma microenvironment in greater detail could help identify valuable therapeutic target combinations for future treatments.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"93-102"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-magnitude high-frequency vibration ameliorates high glucose-induced endothelial injury by restoring mitochondrial function via AMPK/mTOR pathway. 低幅高频振动通过 AMPK/mTOR 途径恢复线粒体功能，从而改善高血糖诱导的内皮损伤。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-06-01 Epub Date: 2024-11-20 DOI: 10.1080/01478885.2024.2429855

Xidan Zhang, Jiyu Sun, Xiting Zhu, Zhenghao Yang, Zhuoli Zhu, Min Zhou, Chen Li, Haiyang Yu, Xueqi Gan

{"title":"Low-magnitude high-frequency vibration ameliorates high glucose-induced endothelial injury by restoring mitochondrial function via AMPK/mTOR pathway.","authors":"Xidan Zhang, Jiyu Sun, Xiting Zhu, Zhenghao Yang, Zhuoli Zhu, Min Zhou, Chen Li, Haiyang Yu, Xueqi Gan","doi":"10.1080/01478885.2024.2429855","DOIUrl":"10.1080/01478885.2024.2429855","url":null,"abstract":"High glucose-induced dysfunction of endothelial cells is a critical and initiating factor in the genesis of diabetic vascular complications. Low-magnitude high-frequency vibration (LMHFV) is a non-invasive biophysical intervention. It has been reported that it exhibits protective effects on high glucose-induced osteoblast dysfunction, but little was known on diabetic vascular complications. In this work, we aim to clarify the role of LMHFV on high glucose-induced endothelial dysfunction and hypothesized that the protective effects functioned through adenosine monophosphate-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway. We cultured primary murine aortic endothelial cells (MAECs) in normal or HG medium, respectively, before exposing to LMHFV. The tube formation, paracellular permeability assay, and aortic ring sprouting assay showed that the high glucose injured-function of MAECs was improved after LMHFV treatment. The intracellular ROS generation analysis, mitochondrial complex I activities measurement, ATP measurement and mitochondrial membrane potential (MMP), and mitochondrial ROS generation analysis of MAECs indicated that mitochondrial function was restored by LMHFV loading in a high glucose environment. Mechanically, western blot assays showed that AMPK phosphorylation was promoted and mTOR was inhibited in LMHFV-induced endothelial function restoration. After the administration of the AMPK inhibitor, Compound C, these protective effects resulting from LMHFV are reversed. These findings suggest that LMHFV plays a significant role in protecting endothelial cells' function and mitochondrial function in high glucose-induced injured MAECs via AMPK/mTOR signalling.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"82-92"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptation of the HistoEnder, an open-source 3D printer for automated transmission electron microscopy grid staining. 对用于自动透射电子显微镜网格染色的开源三维打印机 HistoEnder 进行改装。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-06-01 Epub Date: 2024-09-23 DOI: 10.1080/01478885.2024.2403872

Philip Seifert

引用次数: 0

Hyperglycemia and insulin treatment promote the proliferation of retinal pigment epithelium cells in early diabetes: an in vitro and in vivo study. 高血糖和胰岛素治疗促进早期糖尿病视网膜色素上皮细胞的增殖：一项体外和体内研究。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-05-16 DOI: 10.1080/01478885.2025.2503520

Qiurong Lin, Fan Yang, Xiaofeng Zhu, Haidong Zou, Wei Xu

{"title":"Hyperglycemia and insulin treatment promote the proliferation of retinal pigment epithelium cells in early diabetes: an in vitro and in vivo study.","authors":"Qiurong Lin, Fan Yang, Xiaofeng Zhu, Haidong Zou, Wei Xu","doi":"10.1080/01478885.2025.2503520","DOIUrl":"https://doi.org/10.1080/01478885.2025.2503520","url":null,"abstract":"The retinal pigment epithelium (RPE) is associated with the emergence and development of diabetic retinopathy. Interestingly, a previous clinical study observed that the atrophy of RPE cells surrounding the optic disc in type 1 diabetic children were significantly less pronounced compared to normal children, contradicting current reports. In order to explore the molecular mechanisms behind this contradictory phenomenon, we conducted a series of experiments and reached the following results. First, RPE cells proliferation increased in a glucose concentration-dependent manner in vitro, accompanied by elevated Brachyury and CTGF protein expression, but decreased overall cell viability. Secondly, in vitro experiments and diabetes mouse models confirmed that insulin promoted RPE cell proliferation in high glucose concentrations by activating ERK1/2 phosphorylation. Furthermore, insulin down-regulated the expression of Brachyury and CTGF proteins, possibly reducing high-glucose‒induced damage to RPE cells. In conclusion, the effect of insulin treatment on the proliferation of RPE cells was significantly more significant than that of hyperglycemia, which may be related to the activation of Erk1/2 or reduction of RPE cell damage by inhibiting the occurrence of EMT.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":0.6,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corticoclaustral connections in the cat. 猫的皮质闭侧连接。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-03-14 DOI: 10.1080/01478885.2025.2476835

B Landzhov, D Hinova-Palova, K Fakih, L Edelstein, L Gaydarski, A Alexandrov, V Kirkov, A Paloff, E Radeva

{"title":"Corticoclaustral connections in the cat.","authors":"B Landzhov, D Hinova-Palova, K Fakih, L Edelstein, L Gaydarski, A Alexandrov, V Kirkov, A Paloff, E Radeva","doi":"10.1080/01478885.2025.2476835","DOIUrl":"https://doi.org/10.1080/01478885.2025.2476835","url":null,"abstract":"The claustrum is a sheet-like layer of gray matter situated between the external and extreme capsules of the mammalian brain. This structure was first described by the French physician and anatomist Vicq d'Azyr in 1786. The claustrum's phylogeny, ontogeny and functional characteristics have long been the subject of debate and considerable investigative efforts. However, despite such efforts (or perhaps as a result thereof), significant disparities and discrepancies remain, most notably in the context of the claustrum's afferent and efferent connections. For the purpose of this study, we sought to focus our efforts on fronto-claustral and occipito-claustral connections. Twelve healthy, adult male cats, each weighing ~ 3.5 kg, were studied, seven of which underwent electrolytic lesions of the frontal cortex (A3, A4, and a portion of A6), and five of the occipital cortex (A17, A18, A21). From three to six days after lesioning, subjects were euthanized in accordance with ethical norms. After the brains were removed and blocked, samples of the claustrum were taken and prepared for electron microscopy. Three to six days after lesions of the frontal cortex, we observed an abundance of degenerative boutons in the dorsal claustrum. The vast majority of boutons exhibited the characteristic signs of dark degeneration, whereas only 10% appeared to have undergone light degeneration. Similar results were seen in the dorsal claustrum over the same period of time following lesions of the visual cortex. These results suggest that the dorsal claustrum receives at least two types of connections - separately and concurrently - from the frontal and occipital cortices.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":0.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histogel-based techniques for embedding organoids in paraffin blocks enable high throughput downstream histopathological analyses. 基于组织凝胶的技术可将有机体包埋在石蜡块中，从而实现高通量的下游组织病理学分析。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-03-01 Epub Date: 2024-09-05 DOI: 10.1080/01478885.2024.2398381

Charles Havnar, Loryn Holokai, Ryan Ichikawa, Wennie Chen, Alexis Scherl, Eliah R Shamir

{"title":"Histogel-based techniques for embedding organoids in paraffin blocks enable high throughput downstream histopathological analyses.","authors":"Charles Havnar, Loryn Holokai, Ryan Ichikawa, Wennie Chen, Alexis Scherl, Eliah R Shamir","doi":"10.1080/01478885.2024.2398381","DOIUrl":"10.1080/01478885.2024.2398381","url":null,"abstract":"Organoids are in vitro tissue models derived from human or animal primary tissues or stem cells that allow for studying three-dimensional (3D) tissue biology, toxicity testing, biomarker evaluation, and assessment of compound efficacy, supplementing or potentially minimizing use of animal models. Organoids are typically cultured in a 3D format within an extracellular matrix and, at the end of an experiment, can be further processed for various cellular or molecular readouts. Analysis often relies on whole mount immunolabeling for markers of interest, which consumes the entire sample/well, thereby limiting sample availability for downstream assays. In addition, 3D cultures become more friable after fixation and are susceptible to sample loss during washing steps. In contrast, by fixing and processing organoids to a paraffin block, dozens or hundreds of unstained slides can be generated, enabling robust characterization via multiple assays, including histologic evaluation and (immuno)histochemical stains, thus maximizing the yield of these time- and labor-intensive cultures. Here we describe three methods to process 3D Matrigel cultures into paraffin blocks using Histogel as an embedding agent. The three techniques all yield high-quality sections but vary in complexity of implementation at different steps, and their application for different use cases is discussed.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"46-57"},"PeriodicalIF":0.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological methods for plant tissues. 植物组织的组织学方法。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-03-01 Epub Date: 2024-09-02 DOI: 10.1080/01478885.2024.2397989

Sheila Criswell, Brian Gaylord, Christopher R Pitzer

{"title":"Histological methods for plant tissues.","authors":"Sheila Criswell, Brian Gaylord, Christopher R Pitzer","doi":"10.1080/01478885.2024.2397989","DOIUrl":"10.1080/01478885.2024.2397989","url":null,"abstract":"Although many of the structures and organelles of vegetative cells are comparable to those of animal tissues, significant differences between the two kingdoms require modifications in histological techniques for both tissue processing steps and histochemical staining techniques. The authors investigated the challenges of working with plant tissues by collecting various flora to represent the four main plant organs: leaf, stem, root, and flower/fruit. Triplicate samples for each specimen were placed into formalin for paraffin embedding, placed into formalin for later frozen sections, and used fresh to undergo immediate frozen sectioning. Frozen sections of plant tissues were more difficult to obtain than formalin-fixed paraffin-embedded (FFPE) sections, exhibited tissue loss during staining, and were inferior morphologically to FFPE sections. Although, historically, plant tissue fixation and processing has employed several different reagents compared with those used in animal tissue processing and took significantly longer times, the current investigation determined reagents and protocols from a modern histology laboratory which processes mammalian tissues can be applied to plant tissue processing with only slight modifications in respect to reagent timing. Additionally, staining techniques were compared and while it is well known that plant cell walls stain well with safranin O, the current investigation determined the uptake of safranin O can be accelerated by incubating at 60°C.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"58-67"},"PeriodicalIF":0.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resolving the bone - optimizing decalcification in spatial transcriptomics and molecular pathology. 在空间转录组学和分子病理学中解决骨优化脱钙问题。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2025-03-01 Epub Date: 2024-12-26 DOI: 10.1080/01478885.2024.2446038

Shuoshuo Wang

引用次数: 0