Journal of Histotechnology最新文献

Adaptation of the HistoEnder, an open-source 3D printer for automated transmission electron microscopy grid staining. 对用于自动透射电子显微镜网格染色的开源三维打印机 HistoEnder 进行改装。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-23 DOI: 10.1080/01478885.2024.2403872

Philip Seifert

引用次数: 0

A study of PGP 9.5 immunohistochemical labeling on formalin-fixed paraffin embedded tissues for epidermal nerve fiber density testing 对用于表皮神经纤维密度测试的福尔马林固定石蜡包埋组织进行 PGP 9.5 免疫组化标记的研究

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2024-09-17 DOI: 10.1080/01478885.2024.2405418

Joseph A. Esposito, Camryn J. Vader, Joel R. Israel, Steve A. McClain

引用次数: 0

Histogel-based techniques for embedding organoids in paraffin blocks enable high throughput downstream histopathological analyses. 基于组织凝胶的技术可将有机体包埋在石蜡块中，从而实现高通量的下游组织病理学分析。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-05 DOI: 10.1080/01478885.2024.2398381

Charles Havnar, Loryn Holokai, Ryan Ichikawa, Wennie Chen, Alexis Scherl, Eliah R Shamir

{"title":"Histogel-based techniques for embedding organoids in paraffin blocks enable high throughput downstream histopathological analyses.","authors":"Charles Havnar, Loryn Holokai, Ryan Ichikawa, Wennie Chen, Alexis Scherl, Eliah R Shamir","doi":"10.1080/01478885.2024.2398381","DOIUrl":"https://doi.org/10.1080/01478885.2024.2398381","url":null,"abstract":"Organoids are in vitro tissue models derived from human or animal primary tissues or stem cells that allow for studying three-dimensional (3D) tissue biology, toxicity testing, biomarker evaluation, and assessment of compound efficacy, supplementing or potentially minimizing use of animal models. Organoids are typically cultured in a 3D format within an extracellular matrix and, at the end of an experiment, can be further processed for various cellular or molecular readouts. Analysis often relies on whole mount immunolabeling for markers of interest, which consumes the entire sample/well, thereby limiting sample availability for downstream assays. In addition, 3D cultures become more friable after fixation and are susceptible to sample loss during washing steps. In contrast, by fixing and processing organoids to a paraffin block, dozens or hundreds of unstained slides can be generated, enabling robust characterization via multiple assays, including histologic evaluation and (immuno)histochemical stains, thus maximizing the yield of these time- and labor-intensive cultures. Here we describe three methods to process 3D Matrigel cultures into paraffin blocks using Histogel as an embedding agent. The three techniques all yield high-quality sections but vary in complexity of implementation at different steps, and their application for different use cases is discussed.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A best practices framework for spatial biology studies in drug discovery and development: enabling successful cohort studies using digital spatial profiling. 药物发现和开发中空间生物学研究的最佳实践框架：利用数字空间剖析成功开展队列研究。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-03 DOI: 10.1080/01478885.2024.2391683

David Krull, Premi Haynes, Anil Kesarwani, Julien Tessier, Benjamin J Chen, Kelly Hunter, Deniliz Rodriguez, Yan Liang, Jim Mansfield, Maxine McClain, Corinne Ramos, Edward Bonnevie, Esperanza Anguiano

{"title":"A best practices framework for spatial biology studies in drug discovery and development: enabling successful cohort studies using digital spatial profiling.","authors":"David Krull, Premi Haynes, Anil Kesarwani, Julien Tessier, Benjamin J Chen, Kelly Hunter, Deniliz Rodriguez, Yan Liang, Jim Mansfield, Maxine McClain, Corinne Ramos, Edward Bonnevie, Esperanza Anguiano","doi":"10.1080/01478885.2024.2391683","DOIUrl":"https://doi.org/10.1080/01478885.2024.2391683","url":null,"abstract":"The discovery of biomarkers, essential for successful drug development, is often hindered by the limited availability of tissue samples, typically obtained through core needle biopsies. Standard 'omics platforms can consume significant amounts of tissue, forcing scientist to trade off spatial context for high-plex assays, such as genome-wide assays. While bulk gene expression approaches and standard single-cell transcriptomics have been valuable in defining various molecular and cellular mechanisms, they do not retain spatial context. As such, they have limited power in resolving tissue heterogeneity and cell-cell interactions. Current spatial transcriptomics platforms offer limited transcriptome coverage and have low throughput, restricting the number of samples that can be analyzed daily or even weekly. While the Digital Spatial Profiling (DSP) method does not provide single-cell resolution, it presents a significant advancement by enabling scalable whole transcriptome and ultrahigh-plex protein analysis from distinct tissue compartments and structures using a single tissue slide. These capabilities overcome significant constraints in biomarker analysis in solid tissue specimens. These advancements in tissue profiling play a crucial role in deepening our understanding of disease biology and in identifying potential therapeutic targets and biomarkers. To enhance the use of spatial biology tools in drug discovery and development, the DSP Scientific Consortium has created best practices guidelines. These guidelines, built on digital spatial profiling data and expertise, offer a practical framework for designing spatial studies and using current and future spatial biology platforms. The aim is to improve tissue analysis in all research areas supporting drug discovery and development.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological methods for plant tissues. 植物组织的组织学方法。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-02 DOI: 10.1080/01478885.2024.2397989

Sheila Criswell, Brian Gaylord, Christopher R Pitzer

{"title":"Histological methods for plant tissues.","authors":"Sheila Criswell, Brian Gaylord, Christopher R Pitzer","doi":"10.1080/01478885.2024.2397989","DOIUrl":"https://doi.org/10.1080/01478885.2024.2397989","url":null,"abstract":"Although many of the structures and organelles of vegetative cells are comparable to those of animal tissues, significant differences between the two kingdoms require modifications in histological techniques for both tissue processing steps and histochemical staining techniques. The authors investigated the challenges of working with plant tissues by collecting various flora to represent the four main plant organs: leaf, stem, root, and flower/fruit. Triplicate samples for each specimen were placed into formalin for paraffin embedding, placed into formalin for later frozen sections, and used fresh to undergo immediate frozen sectioning. Frozen sections of plant tissues were more difficult to obtain than formalin-fixed paraffin-embedded (FFPE) sections, exhibited tissue loss during staining, and were inferior morphologically to FFPE sections. Although, historically, plant tissue fixation and processing has employed several different reagents compared with those used in animal tissue processing and took significantly longer times, the current investigation determined reagents and protocols from a modern histology laboratory which processes mammalian tissues can be applied to plant tissue processing with only slight modifications in respect to reagent timing. Additionally, staining techniques were compared and while it is well known that plant cell walls stain well with safranin O, the current investigation determined the uptake of safranin O can be accelerated by incubating at 60°C.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survey of NAACLS accredited histotechnology programs in the United States. 美国经 NAACLS 认证的组织技术课程调查。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-03-18 DOI: 10.1080/01478885.2024.2327095

Carol Bain, Debra Wood, Sheila Criswell

{"title":"Survey of NAACLS accredited histotechnology programs in the United States.","authors":"Carol Bain, Debra Wood, Sheila Criswell","doi":"10.1080/01478885.2024.2327095","DOIUrl":"10.1080/01478885.2024.2327095","url":null,"abstract":"Histotechnology educational programs are accredited by the National Accrediting Agency for Clinical Laboratory Sciences (NAACLS) and currently number fewer than 50 in the United States which contributes to the shortages of laboratory personnel. A survey tool designed with REDCap software was distributed to all programs identified on the NAACLS website and consisted of three parts: a) program information, b) facility information, and c) challenges. Programs are located primarily in large urban centers where populations are most concentrated. The median class size was 6 which may explain the excellent student outcomes to include 96% graduation rates and 90.7% board of registry examination pass rates. Overall, programs had ample equipment, funding, and administrative support. Costs to attend the programs were relatively low (<$3,000 per semester) for over half of the programs. However, due to the small number of accredited education programs across the US, potential students do not often have access to an institution in their area. The programs indicated that the most common challenge was recruitment of adequate high-quality candidates which may explain, in part, the persistent shortage of personnel in the histology laboratory.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140143574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of light and electron microscopic morphometric parameters of glomerular capillaries with serum creatinine and proteinuria. 肾小球毛细血管的光镜和电子显微镜形态参数与血清肌酐和蛋白尿的相关性。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-03-11 DOI: 10.1080/01478885.2024.2326274

Dibyajyoti Boruah, A W Kashif, Barun Kumar Chakrabarty, Sarika Harikrishnan, Arijit Sen

{"title":"Correlation of light and electron microscopic morphometric parameters of glomerular capillaries with serum creatinine and proteinuria.","authors":"Dibyajyoti Boruah, A W Kashif, Barun Kumar Chakrabarty, Sarika Harikrishnan, Arijit Sen","doi":"10.1080/01478885.2024.2326274","DOIUrl":"10.1080/01478885.2024.2326274","url":null,"abstract":"Waste products in the bloodstream are filtered by the glomerular capillaries in the kidneys and excreted into the urine. When making a differential diagnosis of kidney diseases, structural assessment of glomeruli using histological, ultrastructural, and immunological studies is crucial. This study assessed the microscopic and ultrastructural morphometric parameters of glomerular capillaries and examined their correlation with serum creatinine and proteinuria. A total of 60 kidney biopsy cases received by the transmission electron microscope (TEM) laboratory for diagnosis were included in the study. Toluidine blue stained 300 nm thick sections of TEM tissue blocks were scanned for glomerular morphometry by a whole slide imaging system, and the estimation of Bowman's capsule (BC) area, glomerular capillary lumen diameter (GCLD), glomerular capillary density (GCD), glomerular capillary surface area density (GCSA), and percentage of glomerular capillary lumen space (%GCLS) was performed with QuPath software. TEM images of 70 nm thick sections were used for the evaluation of endothelial fenestration diameter (EFD), glomerular basement membrane (GBM) thickness, and podocyte foot process (PFP) effacement. Proteinuria and serum creatinine showed positive correlations with GBM thickness and PFP effacement. Negative correlations of serum creatinine were observed with EFD, %GCLS, and GCSA. Hence, glomerular filtration is greatly affected by the total area of the glomerular capillary surface and structural changes of GBM. Reduction of glomerulus filtration due to foot process effacement and thickening of GBM results in damage to the filtration barrier leading to the leakage of plasma protein into urine.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of degenerated synaptic boutons in the dorsal claustrum of the cat after electrolytic lesions of the intralaminar thalamic nuclei. 鉴定丘脑内核电解损伤后猫背侧丘脑中退化的突触突触。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-04-02 DOI: 10.1080/01478885.2024.2335827

Dimka Hinova-Palova, Boycho Landzhov, Lawrence Edelstein, Khodor Fakih, Alexandar Alexandrov, Teodora Kiriakova, Elka Radeva, Lyubomir Gaydarski, Frank Denaro, Adrian Paloff

{"title":"Identification of degenerated synaptic boutons in the dorsal claustrum of the cat after electrolytic lesions of the intralaminar thalamic nuclei.","authors":"Dimka Hinova-Palova, Boycho Landzhov, Lawrence Edelstein, Khodor Fakih, Alexandar Alexandrov, Teodora Kiriakova, Elka Radeva, Lyubomir Gaydarski, Frank Denaro, Adrian Paloff","doi":"10.1080/01478885.2024.2335827","DOIUrl":"10.1080/01478885.2024.2335827","url":null,"abstract":"The aim of this study is to investigate whether the dorsal claustrum receives afferent input from the intralaminar thalamic nuclei - centromedian nucleus, central lateral nucleus and paracentral nucleus. The intralaminar thalamic nuclei of eight cats were electrolytically lesioned. We obtained samples from the dorsal claustrum for electron microscopic analysis from the second to the seventh post-procedural day. Two types of degenerated synaptic boutons were observed: electron-dense which formed the majority of boutons, and electron-lucent comprising the remaining samples. Between the second and seventh post-procedural day, we observed a steady increase in the number of electron-dense boutons which were diffusely distributed throughout the dorsal claustrum. Electron-dense degenerated boutons formed asymmetrical contacts with dendritic spines as well as with small and medium-sized dendrites. In contrast, electron-lucent degenerated boutons were observed in earlier post-procedural periods and formed symmetrical axodendritic contacts.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What will it take for histologists to be recognized under CLIA? 组织学家如何才能获得 CLIA 的认可？

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-08-30 DOI: 10.1080/01478885.2024.2391636

Elizabeth Chlipala, Tim Morken, Clare Thornton, Luis Chiriboga

引用次数: 0

Tissue clearing and three-dimensional imaging of intact tissues: a review on FACT protocol. 完整组织的组织清除和三维成像：FACT 方案综述。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-09-01 Epub Date: 2024-05-16 DOI: 10.1080/01478885.2024.2352695

Zohreh Farrar, Alireza Afshar, Afshin Zare, Nadiar M Mussin, Asset A Kaliyev, Kulyash R Zhilisbayeva, Mahdi Mahdipour, Amin Tamadon

{"title":"Tissue clearing and three-dimensional imaging of intact tissues: a review on FACT protocol.","authors":"Zohreh Farrar, Alireza Afshar, Afshin Zare, Nadiar M Mussin, Asset A Kaliyev, Kulyash R Zhilisbayeva, Mahdi Mahdipour, Amin Tamadon","doi":"10.1080/01478885.2024.2352695","DOIUrl":"10.1080/01478885.2024.2352695","url":null,"abstract":"FACT is a developed technique for clearing tissues that does not use acrylamide. Since the removal of lipids is crucial for transparency and efficient antibody staining throughout the tissue, especially for microscopy and imaging, it is a harmful process that can cause the loss of important biological molecules such as proteins. The FACT technique overcomes this by chemically bonding the membrane and intracellular proteins with the extracellular matrix, creating a massive 3D hydrogel matrix and providing structural support to fortify the tissue during processing. Compared to other acrylamide-based techniques, the FACT technique requires less labor and harmful chemicals and is therefore considered a more suitable option. In this study, we describe the complete FACT protocol for antibody staining and imaging of whole-cleared tissues while preserving structure and improving image quality. The protocol includes tissue perfusion, fixation, clearing, antibody staining, refractive index matching (RIM) (), microscopy, and imaging. The timing for each step varies depending on the size, weight, and type of tissue, as well as the type of immunostaining. We provide an example of the FACT protocol using mouse brain tissue, which demonstrates its suitability for molecular interrogation analysis of large tissues. The FACT technique has been successfully performed on different types of tissues, making it a favorable choice for a variety of applications.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140944901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0