Journal of Histotechnology最新文献

Optimizing tissue adherence on glass slides using polyurethane glue: a new slide preparation method.

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-17 DOI: 10.1080/01478885.2024.2440679

Jeffery T Williams, Tracy A Goodpaster, Miki Haraguchi

引用次数: 0

Multiple levels of Gomori methenamine silver (GMS) stains do not improve diagnostic yield in esophageal biopsies.

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-12 DOI: 10.1080/01478885.2024.2437587

Michael Occidental, Andrew Dunn, Aaron R Huber

引用次数: 0

Optimizing immunofluorescent staining of H vessels within an irradiated fracture callus in paraffin-embedded tissue samples. 优化石蜡包埋组织样本中辐照骨折茧内 H 血管的免疫荧光染色。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-01 Epub Date: 2024-07-03 DOI: 10.1080/01478885.2024.2371060

Nathan T Sheppard, Melissa C Daniel, Noah S Nelson, Alexis Donneys, Steven R Buchman

{"title":"Optimizing immunofluorescent staining of H vessels within an irradiated fracture callus in paraffin-embedded tissue samples.","authors":"Nathan T Sheppard, Melissa C Daniel, Noah S Nelson, Alexis Donneys, Steven R Buchman","doi":"10.1080/01478885.2024.2371060","DOIUrl":"10.1080/01478885.2024.2371060","url":null,"abstract":"H vessels are an essential link in angiogenic-osteogenic coupling and orchestrate the process of bone healing. H vessels are critically deficient in the setting of radiation-induced fractures, which have been reported to occur in up to 25% of patients undergoing radiotherapy. By increasing H-vessel proliferation, Deferoxamine (DFO) revitalizes the physiologic response to skeletal injury and accelerates irradiated fracture repair. H-vessel quantification is therefore an important outcome measure in histologic analysis of bone healing. However, an optimized protocol for staining H vessels in formalin-fixed paraffin-embedded (FFPE) tissue sections has not been reported. With this protocol, we describe a method of staining FFPE bone samples with minimal background fluorescence and high signal-to-noise ratio. We examined mandibular specimens in a rat model of bone healing from a range of fracture conditions, including healthy bone (Fx), irradiated bone (XFx), and irradiated bone with DFO treatment (XFx-DFO). Quantitative analysis revealed a significant increase of H vessels in the XFxDFO group compared to both the Fx and XFx groups. By optimizing immunofluorescent staining of H vessels in FFPE samples across a range of fracture conditions, we offer investigators an efficacious means of producing reliable imaging for quantitative analysis of H vessels in an irradiated fracture callus.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"173-179"},"PeriodicalIF":0.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141492250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of in vivo bio-integration of three hyaluronic acid-based fillers for 26 weeks: a histological study. 三种透明质酸类填充剂 26 周体内生物整合比较分析：组织学研究。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-01 Epub Date: 2024-07-18 DOI: 10.1080/01478885.2024.2369967

Baoji Song, Qiqi Chen

引用次数: 0

Novel processing and staining methodology of bottlenose dolphin (Tursiops truncatus) teeth for age determination. 用于年龄测定的瓶鼻海豚（Tursiops truncatus）牙齿新处理和染色方法。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-01 Epub Date: 2024-03-11 DOI: 10.1080/01478885.2024.2327155

Christa E Barrett, Ian Evans, Timothy Morgan, Alicia K Olivier

{"title":"Novel processing and staining methodology of bottlenose dolphin (Tursiops truncatus) teeth for age determination.","authors":"Christa E Barrett, Ian Evans, Timothy Morgan, Alicia K Olivier","doi":"10.1080/01478885.2024.2327155","DOIUrl":"10.1080/01478885.2024.2327155","url":null,"abstract":"Age determination of bottlenose dolphins (Tursiops truncatus) is a critical tool in understanding both individual and population health. There are many methods of aging bottlenose dolphins including analysis of teeth, pectoral flipper radiographs, and epigenetics. The most common and oldest method for aging toothed cetaceans is the counting of growth layer groups (GLGs) in the teeth. Current techniques have technical and repeatability challenges. Therefore, a processing technique that results in better resolution of GLGs is needed. This study compares different decalcifications and different histochemical staining techniques. Decalcification was done using 10% EDTA, Kristensen's decalcification, and Rapid Decalcification Solution (RDO). Following decalcification and routine processing, GLGs were assessed using Hematoxylin and Eosin (H&E), hematoxylin, Giemsa, Wright-Giemsa, Toluidine Blue (T-Blue), Masson's Trichrome, and Congo Red staining techniques. Decalcification with Kristensen's and staining with Masson's Trichrome and Congo Red were determined to best highlight GLGs. This processing and staining was then applied to a sample population of 102 bottlenose dolphins that were evaluated independently and blindly by two observers. Of the 102 dolphin samples, 13 (12.7%) were unable to age due to no clear distinction or distortion between GLGs.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"146-153"},"PeriodicalIF":0.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Addressing the histotechnologist shortage through improved classification and recognition. 通过改进分类和识别，解决组织技术人员短缺问题。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI: 10.1080/01478885.2024.2424049

Henley Marcelus, Daniel Packert

引用次数: 0

C5aR expression in kidney tubules, macrophages and fibrosis. 肾小管、巨噬细胞和纤维化中的 C5aR 表达。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-11-28 DOI: 10.1080/01478885.2024.2430041

Carolyn Dunlap, Niky Zhao, Linda S Ertl, Thomas J Schall, Kathleen M C Sullivan

{"title":"C5aR expression in kidney tubules, macrophages and fibrosis.","authors":"Carolyn Dunlap, Niky Zhao, Linda S Ertl, Thomas J Schall, Kathleen M C Sullivan","doi":"10.1080/01478885.2024.2430041","DOIUrl":"https://doi.org/10.1080/01478885.2024.2430041","url":null,"abstract":"The anaphylatoxin C5a and its receptor C5aR (CD88) are complement pathway effectors implicated in renal diseases, including ANCA-associated vasculitis. We investigated the kidney expression of C5aR and a second C5a receptor C5L2 by using immunohistochemistry, in situ hybridization, and spatial gene expression on formalin-fixed, paraffin-embedded human and mouse kidney. C5aR was detected on interstitial macrophages and in multiple tubular regions, including distal and proximal; C5L2 had a similar expression pattern. The 5/6 nephrectomy model of chronic kidney injury exhibited increased C5aR expression by infiltrating cells within the fibrotic regions. C5aR expression was confirmed on human leukocytes and in vitro differentiated macrophages by flow cytometry, and treatment with C5a induced the expression of chemokines and remodeling factors by macrophages, including CCL-3/-4/-7, -20, MMP-1/-3/-8/-12, and F3, and promoted leukocyte survival. C5a activity was C5aR dependent, as demonstrated by reversal with the C5aR inhibitor avacopan. Collectively, these results suggest that myeloid C5aR may induce excessive inflammation in the kidney via immune cell recruitment, extracellular matrix destruction, and remodeling, resulting in fibrotic tissue deposition.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-19"},"PeriodicalIF":0.6,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low-magnitude high-frequency vibration ameliorates high glucose-induced endothelial injury by restoring mitochondrial function via AMPK/mTOR pathway. 低幅高频振动通过 AMPK/mTOR 途径恢复线粒体功能，从而改善高血糖诱导的内皮损伤。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-11-20 DOI: 10.1080/01478885.2024.2429855

Xidan Zhang, Jiyu Sun, Xiting Zhu, Zhenghao Yang, Zhuoli Zhu, Min Zhou, Chen Li, Haiyang Yu, Xueqi Gan

{"title":"Low-magnitude high-frequency vibration ameliorates high glucose-induced endothelial injury by restoring mitochondrial function via AMPK/mTOR pathway.","authors":"Xidan Zhang, Jiyu Sun, Xiting Zhu, Zhenghao Yang, Zhuoli Zhu, Min Zhou, Chen Li, Haiyang Yu, Xueqi Gan","doi":"10.1080/01478885.2024.2429855","DOIUrl":"https://doi.org/10.1080/01478885.2024.2429855","url":null,"abstract":"High glucose-induced dysfunction of endothelial cells is a critical and initiating factor in the genesis of diabetic vascular complications. Low-magnitude high-frequency vibration (LMHFV) is a non-invasive biophysical intervention. It has been reported that it exhibits protective effects on high glucose-induced osteoblast dysfunction, but little was known on diabetic vascular complications. In this work, we aim to clarify the role of LMHFV on high glucose-induced endothelial dysfunction and hypothesized that the protective effects functioned through adenosine monophosphate-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathway. We cultured primary murine aortic endothelial cells (MAECs) in normal or HG medium, respectively, before exposing to LMHFV. The tube formation, paracellular permeability assay, and aortic ring sprouting assay showed that the high glucose injured-function of MAECs was improved after LMHFV treatment. The intracellular ROS generation analysis, mitochondrial complex I activities measurement, ATP measurement and mitochondrial membrane potential (MMP), and mitochondrial ROS generation analysis of MAECs indicated that mitochondrial function was restored by LMHFV loading in a high glucose environment. Mechanically, western blot assays showed that AMPK phosphorylation was promoted and mTOR was inhibited in LMHFV-induced endothelial function restoration. After the administration of the AMPK inhibitor, Compound C, these protective effects resulting from LMHFV are reversed. These findings suggest that LMHFV plays a significant role in protecting endothelial cells' function and mitochondrial function in high glucose-induced injured MAECs via AMPK/mTOR signalling.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-11"},"PeriodicalIF":0.6,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic yield of stains for infectious organisms in esophageal or gastroesophageal junction biopsies with esophagitis. 食管炎食管或胃食管交界处活检中感染性生物体染色的诊断率。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-10-14 DOI: 10.1080/01478885.2024.2415154

Aaron R Huber, Christa L Whitney-Miller

引用次数: 0

The impact of gliomas on the normal brain microenvironment: a pilot study. 胶质瘤对正常大脑微环境的影响：一项试点研究。

IF 0.6 4区生物学

Journal of Histotechnology Pub Date : 2024-10-01 DOI: 10.1080/01478885.2024.2408505

Nicole Riha, Jacen S Moore, Sheila Criswell

{"title":"The impact of gliomas on the normal brain microenvironment: a pilot study.","authors":"Nicole Riha, Jacen S Moore, Sheila Criswell","doi":"10.1080/01478885.2024.2408505","DOIUrl":"https://doi.org/10.1080/01478885.2024.2408505","url":null,"abstract":"Gliomas are malignant tumors of neuronal support cells within the central nervous system (CNS) and are characterized by poor overall prognoses and limited treatment options due to their infiltrative growth patterns. The neural tumor microenvironment, composed of benign neurons, neuroglia, endothelial cells, and intravascular white blood cells, is a target-rich site for potential chemotherapeutic agents. This study assessed cell proliferation rates, white blood cell components, and a limited number of nuclear, cytoplasmic, and membrane markers using immunohistochemistry (IHC) assays on formalin-fixed and paraffin-embedded benign and glial tumor tissue samples from the CNS. It was observed that glioma tissues had increased rates of glial cell proliferation and significant increases in the number of observed T-lymphocytes and granulocytes but decreased expression of markers Somatostatin receptor 2 (SSTR2), L1 cell adhesion molecule (L1CAM), and GATA binding protein 3 (GATA3) when compared to benign tissue samples. Understanding the lack of protein expression and population expansion potential of the glioma microenvironment in greater detail could help identify valuable therapeutic target combinations for future treatments.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":0.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0