Journal of Histotechnology最新文献

{"title":"Evaluation of alternative pretreatment solutions for the Masson's trichrome stain: replacements for Bouin's solution.","authors":"Amber Moser, Hannah Benton, Elizabeth Chlipala, Sheila Criswell","doi":"10.1080/01478885.2026.2748813","DOIUrl":"https://doi.org/10.1080/01478885.2026.2748813","url":null,"abstract":"<p><p>Masson's trichrome stain is a standard stain used to differentiate extracellular matrix components, primarily collagen, in histologic tissue samples. The classic pretreatment solution, Bouin's solution, contains formaldehyde, picric acid, and acetic acid. The chemical components introduce safety concerns encompassing toxicity, respiratory, explosive hazards, and environmental hazards. Three pretreatment solutions (citric acid solutions pH 4 and pH 6, and Gram's iodine) as well as a no pretreatment condition, were assayed alongside Bouin's solution on various human and animal tissue samples by two laboratories using manual methods. The investigators determined that tissue sections heated at 60-70°C for 30 min in either Gram's iodine or citric acid solution pH 4 produced similar staining results to those using Bouin's solution and therefore may be safe and effective replacement solutions for the trichrome stain.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-7"},"PeriodicalIF":1.9,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149220417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A method of resin processing and analysis for ovine bone-tendon-implant complex.","authors":"C Broomfield, B Gadomski, D von Stade","doi":"10.1080/01478885.2026.2701906","DOIUrl":"https://doi.org/10.1080/01478885.2026.2701906","url":null,"abstract":"<p><p>Sheep are an accepted model for translational orthopaedic research and histological analysis is a main outcome for many of the studies. Standard paraffin techniques are not appropriate when the tissue has an implant, suture, or other structure that is not supported by paraffin. Resin histology is a technique that supports the implant and permits analysis of the implant/tissue interface. Methyl methacrylate is a common resin choice and has been well documented, but there is a lack of information concerning processing techniques on ovine bone-tendon-implant complexes. Ovine bone-tendon-implant are large (2 cm × 5 cm × 0.4 cm) and have dense fibrous tissue, making them difficult to process and section. This technical note describes a method of grossing, processing, and staining, that produces high‑quality ground slides that allow the pathologist to interpret what is happening at the cellular level.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-6"},"PeriodicalIF":1.9,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149074779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathology turnaround time analysis: identifying bottlenecks and implementing mitigation strategies in a multi-site laboratory.","authors":"Temple C Evoegbulem, Richard P O Jones, Rony A Lopez, Uzochukwu A Ononuju, Anderline Ogechi Aguanunu","doi":"10.1080/01478885.2026.2731824","DOIUrl":"https://doi.org/10.1080/01478885.2026.2731824","url":null,"abstract":"<p><p>Turnaround time (TAT) is a critical quality metric in histopathology laboratories, directly influencing clinical decision-making and patient outcomes. Despite its importance, the specific workflow stages responsible for TAT prolongation in a laboratory serving multiple hospitals could be better characterized. This study aimed to evaluate histopathology laboratory TAT at Brown University Health-Rhode Island Hospital (BUH-RIH), identify workflow bottlenecks, and propose evidence-based mitigation strategies. A retrospective observational study was conducted using a systematic random sample of 97 cases (556 tissue blocks) from a total of 16,227 blocks received during January 2026. Data were extracted from the COPATH laboratory information management system and specimen logs. TAT was defined as the interval between specimen receipt and final report release, excluding weekends. Descriptive statistical analyses were performed using Microsoft Excel and Tableau. Overall, 87.5% of diagnostic biopsy cases were reported within the laboratory benchmark of ≤2 days, and 81.3% of routine surgical cases met the ≤5-day target. Average TAT was 1.6 days for the overall diagnostic biopsies and 3.3 days for the overall routine surgical specimens. The pathologist sign-out stage was the largest contributor to total TAT (1.7 days). Ancillary testing increased average TAT to 2.4 days for diagnostic biopsies and 5.3 days for routine surgical specimens. Inter-hospital and temporal variations were also observed. While overall TAT performance was satisfactory, sign-out and ancillary testing represent the principal bottlenecks. Structured case allocation, defined sign-out schedules, protocol-based early ordering of spare slides, digital pathology adoption, and optimized staffing are recommended to enhance laboratory efficiency and patient care.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-12"},"PeriodicalIF":1.9,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148989313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term diet-induced obesity disrupts testicular activity in Wistar rats: biochemical, hormonal, and structural evidence.","authors":"Samira Amroun, Assia Kamel-Chouider, Amel Ghozlani, Hanane Akli, Zohra Hamouli-Said","doi":"10.1080/01478885.2026.2725268","DOIUrl":"https://doi.org/10.1080/01478885.2026.2725268","url":null,"abstract":"<p><p>The increasing prevalence of obesity has been closely associated with male reproductive disorders. Studies on diet-induced obesity provided valuable insights into the factors involved in the decline of male reproductive potential in obese subjects. The aim of the present study was to investigate the consequences of long-term exposure to cafeteria diet, particularly on testicular activity, and pinpoint the most affected components by this condition. The study was conducted on male Wistar rats fed either a standard chow or a cafeteria diet during 16 weeks. A model of diet-induced obesity was assessed, and anthropometrical measures, hormonal, biochemical, and redox profiles were evaluated. Histological, transmission electron microscopic (TEM) and morphometric analysis of the testes as well as the immunolabelling of AR were carried out. DNA fragmentation index was also measured in both groups. The results showed an increase in body weight correlated with elevated BMI and Lee Index values. The elevated fat coefficient was also associated with unbalanced hormonal profile and biochemical parameters. Our histological findings showed altered cytoarchitecture, including sloughing of germ cells into the lumen, and degenerative organelles, along with morphometric measurement changes and decreased immunolabelling of androgen receptors. In addition, oxidative stress markers and DNA fragmentation index increased in the cafeteria diet fed group. Long-term exposure to cafeteria diet had significant impact on testicular function, particularly on structural integrity of seminiferous tubules. Chronic hyperglycemia, insulin resistance and oxidative stress appear to be key factors contributing to the impairment of the male reproductive potential.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-15"},"PeriodicalIF":1.9,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148989345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compartment-specific collagen architecture in Crohn's disease biopsies using SHG/TPE microscopy: a pilot feasibility study.","authors":"Raseen Tariq, Bryce Hatfield, Kutbuddin Akbary, Ren Yayun, Tejal Patki, Shailaja L Reddy, Arun J Sanyal","doi":"10.1080/01478885.2026.2728289","DOIUrl":"https://doi.org/10.1080/01478885.2026.2728289","url":null,"abstract":"<p><p>Intestinal fibrosis in Crohn's disease (CD) is a key determinant of stricture formation, but collagen architecture is difficult to assess on routine mucosal biopsies because of limited tissue depth and the lack of quantitative tissue-based fibrosis measures. We evaluated the feasibility of applying second harmonic generation/two-photon excitation microscopy (SHG/TPE) with qFibrosis analysis to quantify collagen microarchitecture across histologic compartments in CD biopsies. Unstained formalin-fixed paraffin-embedded endoscopic biopsies from 31 samples obtained from 20 patients with CD were analyzed using SHG/TPE imaging and qFibrosis. Regions of interest were annotated by a gastrointestinal pathologist into glands/crypts, lamina propria, muscularis mucosae, and submucosa. Quantitative morphometric parameters reflecting collagen burden, fiber/string features, and architectural organization were compared across compartments. SHG/TPE imaging with qFibrosis successfully generated compartment-level collagen morphometric data from routine FFPE biopsy tissue. Collagen content (%SHG) and aggregated collagen (%Agg) increased progressively with tissue depth, with the highest levels observed in the submucosa. In contrast, superficial compartments showed higher fiber/string-based parameters, suggesting a more dispersed collagen architecture. These findings demonstrate measurable, depth-dependent differences in collagen organization across intestinal biopsy compartments, including patterns not readily appreciable on routine histology. SHG/TPE-based qFibrosis analysis is feasible for quantitative assessment of collagen microarchitecture in routine CD biopsy specimens and identifies compartment-specific, depth-dependent collagen patterns. However, because this pilot study lacked non-IBD controls and paired histochemical validation with Sirius Red or trichrome staining, these findings should not be interpreted as disease-specific or clinically validated. These preliminary findings support further validation of SHG/TPE-based collagen morphometry as a potential tissue-level approach for studying intestinal fibrosis in larger cohorts with controls, paired histochemical validation, histologic correlation, and clinical outcomes.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-5"},"PeriodicalIF":1.9,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148891783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TissuStamp: a novel high-throughput tissue transfer workflow for FFPE spatial biology assays.","authors":"Albert Velasco Abadia, Gina Benedetto, Zhenmin Hong, Jaekyung Koh, Sarthak Duggal, Sandor Kovacs, Mohammad Vatankhah Varnosfaderani, Mark Kalaj, Bandele Jeffrey-Coker, Anthony Facchini, McKenzie Pavlich, Wijendra Jude Senarathne, Digvijay Singh, Patricia Aurelina, Michael Lawson, Sabrina Shore, Kevin Marshall, Yuji Ishitsuka, Eli Glezer, Daan Witters","doi":"10.1080/01478885.2026.2648709","DOIUrl":"10.1080/01478885.2026.2648709","url":null,"abstract":"<p><p>Spatial biology is shifting from single-sample studies to cohort-scale analyses required for translational research, AI model development, and clinical deployment, yet tissue preparation methods have remained largely unchanged for decades, creating a critical throughput bottleneck. Existing approaches face a trade-off: traditional processing accommodates only a few samples per slide, while tissue microarrays increase sample count by restricting analysis to small cores that limit spatial context. This technical note describes TissuStamp™, a tissue transfer system that enables high-throughput FFPE tissue preparation for spatial multiomics. FFPE sections are first mounted on gel pads, then specific regions of interest are punched using square blades and 'stamped' onto surface-functionalized TissuGrip™ slides using a pusher block. This enables the transfer of up to 32 millimeter-scale tissue regions or 10 larger regions (totaling up to ~1,000 mm² of tissue area) onto a single standard 75 mm × 25 mm slide in a tightly packed, multi-lane array format. TissuStamp allows tissue cutting, storage and transfer to occur at different times and locations, facilitating multi-institutional studies and biobank initiatives. While TissuStamp was developed for the G4X™ platform, which performs integrated spatial transcriptomic, proteomic, and fluorescent H&E (fH&E™) profiling on up to 128 samples per run at subcellular resolution, its capabilities are transferable to other tissue analysis workflows. Comparative analysis demonstrated that TissuStamp preserved tissue morphology and delivered equivalent multiomic assay performance compared to conventional direct mounting onto slides. This method provides a practical solution for scaling spatial multiomics to cohort-scale research and clinical applications.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"185-198"},"PeriodicalIF":1.9,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147729139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimisation of Xenium automated in situ sequencing for PAXgene-fixed tissue samples.","authors":"Kenny Roberts, Andrew R Bassett","doi":"10.1080/01478885.2026.2667003","DOIUrl":"10.1080/01478885.2026.2667003","url":null,"abstract":"<p><p>Spatial transcriptomics has transformed the study of gene expression in tissue samples, yet its application to non-standard sample preservation formats beyond fresh frozen and FFPE remains underexplored. PAXgene fixation offers advantages for genomic studies by preserving DNA and RNA without crosslinking but poses challenges for high-quality spatial transcriptomics. Here, we present an optimised workflow for applying Xenium, an automated in situ sequencing platform, to PAXgene-fixed paraffin-embedded (PFPE) tissues. Using our newly developed Xenium Tissue Optimisation (XTO) protocol, we systematically tested permeabilisation conditions across multiple mouse and human tissues. We show that pepsin digestion effectively enhances RNA accessibility in PFPE samples, with tuneable digestion times for tissue-specific optimisation, though a compromise may need to be reached between transcript detection rates and maintaining tissue integrity and morphological staining. Our results indicate that, under optimal conditions, PFPE samples can yield comparable spatial RNA transcript detection to formalin-fixed paraffin-embedded (FFPE) and fresh frozen samples. This study provides a framework for adapting in situ sequencing to PFPE tissues, broadening the applicability of spatial transcriptomics to archival and prospective sample collections.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"142-161"},"PeriodicalIF":1.9,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147987871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multiplex immunostaining method for characterization of immune cells in non-human primate ocular tissue sections with intraocular inflammation.","authors":"Rong Nie, Christina Preece, Joshua Rowe, Swati Gupta, Lydia Andrews-Jones, Margaret Ramos","doi":"10.1080/01478885.2026.2689865","DOIUrl":"https://doi.org/10.1080/01478885.2026.2689865","url":null,"abstract":"<p><p>The administration of biologics to the eyes of non-human primates in non-clinical studies can result in sporadic intraocular inflammation (IOI) in individual animals. When the biologic is a human (foreign) protein being administered to a non‑human primate (NHP), understanding the causes and clinical relevance of IOI can be especially challenging and represent an important data gap needed to advance the development of ocular biologic therapies. This study describes the development and validation of a multiplex immunostaining assay designed to address this need. It includes six markers that differentiate T cells (CD4+, CD8+), T regulatory cells (CD4+, FoxP3+), B cells (CD20+), macrophages (CD68+), and M2 macrophages and microglia cells (CD68+, IBA1+). Preliminary results in representative ocular samples with a biologic drug-induced IOI demonstrated the assay's ability to identify which immune cell types are present and their spatial relationships, which can significantly enhance the understanding of the immunology underlying IOI. This adds valuable interpretive information, facilitating better decision-making to develop safe and effective therapies.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"49 3","pages":"131-141"},"PeriodicalIF":1.9,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An integrated protocol for multiplexed DNA FISH and protein detection in large tissue sections.","authors":"Eleanor M O Roberts, Pranauti Panshikar, Xiaoze Li Wang, Christophe Avenel, Quentin Verron, Eléna Coulier, Magda Bienko, Charlotte Stadler","doi":"10.1080/01478885.2026.2703359","DOIUrl":"10.1080/01478885.2026.2703359","url":null,"abstract":"<p><p>Different omics types such as genomics and proteomics all contribute to deciphering biology. Applying these omics approaches in a spatial context helps reveal biology <i>in situ</i> at a single cell level. Here we present a protocol for the combined multiplexed detection of targeted genes using DNA FISH, and proteins using multiplexed immunofluorescence. The protocol is integrated on the commercial PhenoCycler Fusion-platform and generates one single dataset with gene and protein readout at a single cell level in large tissue sections, allowing for a throughput of thousands to millions of cells. The workflow can be used for characterising malignant cells in large tumor areas based on genetic aberrations, while deciphering the cellular landscape and microenvironment from multiplexed protein detection using immunofluorescence.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"199-215"},"PeriodicalIF":1.9,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148584483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bedrock and cornerstones: histotechnology and the lasting foundations of spatial omics.","authors":"Shuoshuo Wang, Jiwoon Park","doi":"10.1080/01478885.2026.2706370","DOIUrl":"https://doi.org/10.1080/01478885.2026.2706370","url":null,"abstract":"","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"49 3","pages":"129-130"},"PeriodicalIF":1.9,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}