Journal of Histotechnology最新文献

{"title":"Exploring the gap in the Occupational Safety And Health Administration (OSHA) laboratory standard: a literature review and recommendations to enhance histology laboratory safety practices.","authors":"Jeana Earn","doi":"10.1080/01478885.2022.2099720","DOIUrl":"https://doi.org/10.1080/01478885.2022.2099720","url":null,"abstract":"<p><p>This article discusses current available resources with respect to regulatory agencies including the Occupational Safety and Health Administration (OSHA) for determining the requirements placed upon laboratories for handling of hazardous materials. The focus is specific to the histology laboratory and xylene use, and includes a literature review, admixed with historical reference points. Procedures and tasks in the histology laboratory are highlighted in relation to their connection to the quality of the work environment with an emphasis on air quality. Recommendations are provided for maintaining an appropriate work environment for the prevention of potential adverse health effects. The gap within the OSHA Laboratory Standard, i.e. a lack of explanatory language, leaves much open to interpretation regarding fume hood usage with volatile hazardous chemicals. As a result, both the level of safety training and the awareness of good laboratory practices (GLP) for handling volatile hazardous reagents such as xylene can become compromised.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"107-115"},"PeriodicalIF":1.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40608975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Journal of Histotechnology 2022 NSH convention issue.","authors":"Gayle M Callis","doi":"10.1080/01478885.2022.2101238","DOIUrl":"https://doi.org/10.1080/01478885.2022.2101238","url":null,"abstract":"The National Society for Histotechology has always had a convention issue as a hand out to attendees and for NSH members to pick up extra copies. It is nice to know this will happen in 2022 after the 2020 and 2021 virtual conventions. 2022 has been a productive year for JOH with good news that the 2021 Impact Factor is 1.918 and has more than doubled from 0.714 in 2020. This is a good indicator that JOH is doing very well and being recognized with more citations. It no doubt was helped by the fact JOH is now on Pubmed/Medline. For all of those who submitted and now have publications in JOH, you have my heartiest thank you for making the journal more successful than ever in the scientific and medical communities. In my thanks, I also include our peer reviewers and the JOH Editorial Board members. If you want to learn more about journal metrics, go to the JOH website https://www.tandfonline.com/action/ journalInformation and read why metrics is an important tool for for both readers and authors. This issue has articles which deal with three practical issues so often found in an everyday working histology laboratory. Questions concerning cell block preparation are frequently seen on The Block. This is addressed by Clif Chapman in his paper showing an ‘innovative’ standardized method for a cell block procedure and preparation, processing and staining. This paper is well illustrated with photographs and images of sections from these cell blocks. One problem that confronts histotechnicians, often on a daily basis, is poor paraffin processing that requires using a ‘reprocessing’ method to recover valuable tissue in order to have a proper diagnosis. Lunetta and colleagues did a careful study to recreate poor processing and then comparing two reprocessing methods commonly used by laboratories. This is a nice review of the two known reprocessing methods but more important was the simulation of poor processing then performing the reprocessing with the two methods. The evaluation and comparison of the two methods at microtomy and with staining is well documented along with photomicrographs of stained tissue sections both before and after reprocessing. This paper can provide guidelines and aid in choosing a reprocessing method for any histology laboratory. Jeana Earn provided a paper exploring the gap in the OSHA laboratory standard using a literature review along with recommendations to enhance laboratory safety practice. The topic is specific for volatile hazardous chemicals with emphasis on xylene, the importance of good laboratory practices (GLP) and the being familiar with agencies that provide guidelines. Although many histology personnel are already familiar with the OSHA standard, this is a good review to remind laboratory personnel that good safety practices in the histology laboratory are necessary to protect the health of workers especially histotechnicians. The paper on sampling of placentas to diagnose Plasmodium falciparium, the parasite that ca","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"95"},"PeriodicalIF":1.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40518098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Test Your Knowledge.","authors":"Brooke H Dubansky","doi":"10.1080/01478885.2022.2101242","DOIUrl":"https://doi.org/10.1080/01478885.2022.2101242","url":null,"abstract":"","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"130"},"PeriodicalIF":1.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40613966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum glucose, sodium and potassium concentrations in patients with diabetic ketoacidosis.","authors":"Željka Dragila, Dora Rudić, Klara Ćosić, Tatjana Bačun","doi":"10.17392/1471-22","DOIUrl":"10.17392/1471-22","url":null,"abstract":"<p><p>Aim To investigate possible differences in serum glucose and sodium and potassium concentrations with respect to age, gender and severity of diabetic ketoacidosis. Methods Medical records from 1 January 2017 to 31 December 2019 were reviewed and patients with the diagnosis of diabetic ketoacidosis were selected. Results The study included 52 patients. Glucose concentration was significantly higher in the age group of 25-44 and >65 years compared to the group of 18-24 years (p=0.02). Sodium concentration was significantly higher in the age group 18-24 and >65 years compared to groups 25-44 and 45-65 years (p=0.002). Females had significantly higher sodium concentration than males (p=0.002). Potassium concentration was significantly higher in the age group 25-44 years compared to other groups (p=0.01). Males had significantly higher potassium concentration (p =0.01). Conclusion This study showed that significant differences exist in electrolyte concentration between specific age groups, male and female gender as well as DKA severity. Knowing these differences could help clinicians to promptly recognize and treat electrolyte derangements, leading to better outcome of patients with DKA.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87607375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surgical Pathology Review","authors":"S. Lau","doi":"10.1080/01478885.2022.2097610","DOIUrl":"https://doi.org/10.1080/01478885.2022.2097610","url":null,"abstract":"","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"45 1","pages":"129 - 129"},"PeriodicalIF":1.1,"publicationDate":"2022-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41731116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue cryopreservation using the 3M™ Novec™ 7000 freezing coolant offers a comparable and safe alternative to customary coolants.","authors":"Victor Nunez,&nbsp;Ashley Cook,&nbsp;Charles Havnar,&nbsp;Sean Flanagan,&nbsp;Nianfeng Ge,&nbsp;Angela Martzall,&nbsp;Robin E Taylor,&nbsp;Millicent Lu,&nbsp;Oleg Mayba,&nbsp;Oded Foreman","doi":"10.1080/01478885.2021.1993528","DOIUrl":"https://doi.org/10.1080/01478885.2021.1993528","url":null,"abstract":"<p><p>Cryopreserving tissues for histology requires the use of coolants to buffer the sample from liquid nitrogen (LN₂) and to control the rate of temperature decline. Several coolants sharing similar physical characteristics are available on the market; however, commonly used coolants are variably flammable and/or toxic and pose risks to personnel and facilities. The purpose of this study was to compare the performance of three commercially available coolants: hexane, 2-methylbutane (2 M), and 1-methoxyheptafluoropropane (N7000). Fresh mouse tissues were frozen by each method, for their ability to preserve microscopic architecture and to protect RNA from degradation were evaluated and compared to tissue characteristics obtained by direct immersion in LN₂. Our results show that for most tissues, the N7000 freezing coolant provides equal or improved preservation of microscopic architecture. While snap-freezing tissues in LN₂ provides superior RNA protection, no significant differences in RNA quality were seen between tissues frozen in hexane, 2 M, and N7000.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"45 2","pages":"85-91"},"PeriodicalIF":1.1,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39631158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inherited connective tissue diseases highlight macromolecular network interdependences in skin extracellular matrix: a histomorphometric study.","authors":"Raed Lattouf,&nbsp;Antoine Assoumou-Abroh,&nbsp;Ronald Younes,&nbsp;Didier Lutomski,&nbsp;Joseph Bassil,&nbsp;Claudine Blanchet-Bardon,&nbsp;Nada Naaman,&nbsp;Sylvie Changotade,&nbsp;Gaston Godeau,&nbsp;Karim Senni","doi":"10.1080/01478885.2021.2024980","DOIUrl":"https://doi.org/10.1080/01478885.2021.2024980","url":null,"abstract":"<p><p>Mutation of just a single extracellular matrix protein, a receptor or enzyme involved in connective tissue metabolism is sufficient to cause systemic pathologies and failure of tissues that are subjected to strong mechanical stresses. Skin histological and computerized image analyses can provide a good qualitative and quantitative indication of these inherited connective tissue diseases. In this study, skin biopsies from young (10 to 25 years) and middle-aged patients (26 to 50 years) suffering from Ehlers-Danlos syndromes (EDS), Marfan syndrome (MS) or pseudoxanthoma elasticum (PXE) were studied after specific staining of both the collagen and elastic networks. Findings from the histomorphometric analyses conducted on skin sections of the patients with EDS, MS and PXE were compared to skin sections of healthy subjects from the same age groups. Our results show that both the collagen and the elastic networks were affected in all the studied pathological cases, but that the adverse changes to the elastic network in older patients were distinct from the physiological changes observed during aging process for healthy subjects. This degenerative process may be explained by an added phenomenon involving a general connective tissue proteolysis.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"45 2","pages":"66-76"},"PeriodicalIF":1.1,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39900644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of CTAG1B clone EPR13780 versus <i>DDIT3</i> gene rearrangement distinguishes myxoid liposarcoma from its mimics with detection of novel <i>DDIT3</i> gene copy number variations.","authors":"Marwa M Abdelaziz,&nbsp;Hanan Y Tayel,&nbsp;Amany Abdel-Bary,&nbsp;Omnia M Badawy","doi":"10.1080/01478885.2021.2004294","DOIUrl":"https://doi.org/10.1080/01478885.2021.2004294","url":null,"abstract":"<p><p>Myxoid liposarcoma (MLPS) has different patterns that are often difficult to distinguish from other soft tissue lesions. MLPS is characterized by a reciprocal translocation involving the DNA Damage Inducible Transcript 3 gene <i>(DDIT3)</i> that can be detected using fluorescent in situ hybridization (FISH). Recently, the marker for cancer testis antigen 1b (CTAG1B) was found to be expressed in MLPS. The aim of the present study was to assess the potential use immunohistochemistry (IHC) for CTAG1B expression and <i>DDIT3</i> rearrangement to diagnose MLPS and distinguish it from similar lesions. Out of 29 cases including MLPS and its mimics, CTAG1B was expressed in 92.86% of cases of MLPS and 20% of its mimics. <i>DDIT3</i> rearrangement was 100% sensitive and 92.86% specific in distinguishing MLPS from its mimics. The <i>DDIT3</i> rearrangement was found to be more sensitive but less specific than cytoplasmic expression of CTAG1B marker. <i>DDIT3</i> polysomy and amplification were detected in some cases. Therefore, both CTAG1B expression and FISH for <i>DDIT3</i> gene can be used to distinguish MLPS from similar tumors. The use of both immunohistochemistry for CTAG1B in addition to <i>DDIT3</i> gene rearrangement detection by FISH was more specific than using either of them alone. However, the <i>DDIT3</i> gene rearrangement alone was the most sensitive test for distinguishing MLPS from its mimics.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"45 2","pages":"56-65"},"PeriodicalIF":1.1,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39677869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glyoxal fixation: an immunohistochemistry assay evaluation.","authors":"Sheila L Criswell,&nbsp;Savanna Altman,&nbsp;Chelsea Peeler,&nbsp;Taylor Drake,&nbsp;Cynthia Lazar,&nbsp;Samantha Douglas,&nbsp;Victoria DeJarnatt","doi":"10.1080/01478885.2021.1996125","DOIUrl":"https://doi.org/10.1080/01478885.2021.1996125","url":null,"abstract":"<p><p>Analysis of surgical pathology specimens by histological techniques including immunohistochemistry (IHC) assays is a mainstay of disease diagnosis in humans. Neutral buffered formalin (NBF) is currently the primary fixative used, but its use is not without risks due to toxicity and carcinogenicity. Several glyoxal-based fixatives have been commercially produced, are considered safer alternatives to NBF, and produce histochemical staining results comparable to that of tissues fixed in NBF. However, previous studies evaluating IHC assay results in tissues fixed in NBF and glyoxal solutions have indicated mixed results. This study demonstrated that while tissues fixed in NBF were slightly superior to tissues fixed in glyoxal solutions among the 34 antibodies assayed with IHC, all fixative solutions produced results compatible for use in an anatomic pathology laboratory.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"45 2","pages":"77-84"},"PeriodicalIF":1.1,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39815136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Test Your Knowledge","authors":"Brooke H. Dubansky, Glenda F. Hood","doi":"10.1080/01478885.2022.2065734","DOIUrl":"https://doi.org/10.1080/01478885.2022.2065734","url":null,"abstract":"(2022). Test Your Knowledge. Journal of Histotechnology: Vol. 45, No. 2, pp. 93-93.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"60 6","pages":""},"PeriodicalIF":1.1,"publicationDate":"2022-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138504214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}