Journal of Histotechnology最新文献

{"title":"The Journal of Histotechnology 2022 NSH convention issue.","authors":"Gayle M Callis","doi":"10.1080/01478885.2022.2101238","DOIUrl":"https://doi.org/10.1080/01478885.2022.2101238","url":null,"abstract":"The National Society for Histotechology has always had a convention issue as a hand out to attendees and for NSH members to pick up extra copies. It is nice to know this will happen in 2022 after the 2020 and 2021 virtual conventions. 2022 has been a productive year for JOH with good news that the 2021 Impact Factor is 1.918 and has more than doubled from 0.714 in 2020. This is a good indicator that JOH is doing very well and being recognized with more citations. It no doubt was helped by the fact JOH is now on Pubmed/Medline. For all of those who submitted and now have publications in JOH, you have my heartiest thank you for making the journal more successful than ever in the scientific and medical communities. In my thanks, I also include our peer reviewers and the JOH Editorial Board members. If you want to learn more about journal metrics, go to the JOH website https://www.tandfonline.com/action/ journalInformation and read why metrics is an important tool for for both readers and authors. This issue has articles which deal with three practical issues so often found in an everyday working histology laboratory. Questions concerning cell block preparation are frequently seen on The Block. This is addressed by Clif Chapman in his paper showing an ‘innovative’ standardized method for a cell block procedure and preparation, processing and staining. This paper is well illustrated with photographs and images of sections from these cell blocks. One problem that confronts histotechnicians, often on a daily basis, is poor paraffin processing that requires using a ‘reprocessing’ method to recover valuable tissue in order to have a proper diagnosis. Lunetta and colleagues did a careful study to recreate poor processing and then comparing two reprocessing methods commonly used by laboratories. This is a nice review of the two known reprocessing methods but more important was the simulation of poor processing then performing the reprocessing with the two methods. The evaluation and comparison of the two methods at microtomy and with staining is well documented along with photomicrographs of stained tissue sections both before and after reprocessing. This paper can provide guidelines and aid in choosing a reprocessing method for any histology laboratory. Jeana Earn provided a paper exploring the gap in the OSHA laboratory standard using a literature review along with recommendations to enhance laboratory safety practice. The topic is specific for volatile hazardous chemicals with emphasis on xylene, the importance of good laboratory practices (GLP) and the being familiar with agencies that provide guidelines. Although many histology personnel are already familiar with the OSHA standard, this is a good review to remind laboratory personnel that good safety practices in the histology laboratory are necessary to protect the health of workers especially histotechnicians. The paper on sampling of placentas to diagnose Plasmodium falciparium, the parasite that ca","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40518098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Test Your Knowledge.","authors":"Brooke H Dubansky","doi":"10.1080/01478885.2022.2101242","DOIUrl":"https://doi.org/10.1080/01478885.2022.2101242","url":null,"abstract":"","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40613966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surgical Pathology Review","authors":"S. Lau","doi":"10.1080/01478885.2022.2097610","DOIUrl":"https://doi.org/10.1080/01478885.2022.2097610","url":null,"abstract":"","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41731116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tissue cryopreservation using the 3M™ Novec™ 7000 freezing coolant offers a comparable and safe alternative to customary coolants.","authors":"Victor Nunez,&nbsp;Ashley Cook,&nbsp;Charles Havnar,&nbsp;Sean Flanagan,&nbsp;Nianfeng Ge,&nbsp;Angela Martzall,&nbsp;Robin E Taylor,&nbsp;Millicent Lu,&nbsp;Oleg Mayba,&nbsp;Oded Foreman","doi":"10.1080/01478885.2021.1993528","DOIUrl":"https://doi.org/10.1080/01478885.2021.1993528","url":null,"abstract":"<p><p>Cryopreserving tissues for histology requires the use of coolants to buffer the sample from liquid nitrogen (LN₂) and to control the rate of temperature decline. Several coolants sharing similar physical characteristics are available on the market; however, commonly used coolants are variably flammable and/or toxic and pose risks to personnel and facilities. The purpose of this study was to compare the performance of three commercially available coolants: hexane, 2-methylbutane (2 M), and 1-methoxyheptafluoropropane (N7000). Fresh mouse tissues were frozen by each method, for their ability to preserve microscopic architecture and to protect RNA from degradation were evaluated and compared to tissue characteristics obtained by direct immersion in LN₂. Our results show that for most tissues, the N7000 freezing coolant provides equal or improved preservation of microscopic architecture. While snap-freezing tissues in LN₂ provides superior RNA protection, no significant differences in RNA quality were seen between tissues frozen in hexane, 2 M, and N7000.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39631158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inherited connective tissue diseases highlight macromolecular network interdependences in skin extracellular matrix: a histomorphometric study.","authors":"Raed Lattouf,&nbsp;Antoine Assoumou-Abroh,&nbsp;Ronald Younes,&nbsp;Didier Lutomski,&nbsp;Joseph Bassil,&nbsp;Claudine Blanchet-Bardon,&nbsp;Nada Naaman,&nbsp;Sylvie Changotade,&nbsp;Gaston Godeau,&nbsp;Karim Senni","doi":"10.1080/01478885.2021.2024980","DOIUrl":"https://doi.org/10.1080/01478885.2021.2024980","url":null,"abstract":"<p><p>Mutation of just a single extracellular matrix protein, a receptor or enzyme involved in connective tissue metabolism is sufficient to cause systemic pathologies and failure of tissues that are subjected to strong mechanical stresses. Skin histological and computerized image analyses can provide a good qualitative and quantitative indication of these inherited connective tissue diseases. In this study, skin biopsies from young (10 to 25 years) and middle-aged patients (26 to 50 years) suffering from Ehlers-Danlos syndromes (EDS), Marfan syndrome (MS) or pseudoxanthoma elasticum (PXE) were studied after specific staining of both the collagen and elastic networks. Findings from the histomorphometric analyses conducted on skin sections of the patients with EDS, MS and PXE were compared to skin sections of healthy subjects from the same age groups. Our results show that both the collagen and the elastic networks were affected in all the studied pathological cases, but that the adverse changes to the elastic network in older patients were distinct from the physiological changes observed during aging process for healthy subjects. This degenerative process may be explained by an added phenomenon involving a general connective tissue proteolysis.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39900644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of CTAG1B clone EPR13780 versus <i>DDIT3</i> gene rearrangement distinguishes myxoid liposarcoma from its mimics with detection of novel <i>DDIT3</i> gene copy number variations.","authors":"Marwa M Abdelaziz,&nbsp;Hanan Y Tayel,&nbsp;Amany Abdel-Bary,&nbsp;Omnia M Badawy","doi":"10.1080/01478885.2021.2004294","DOIUrl":"https://doi.org/10.1080/01478885.2021.2004294","url":null,"abstract":"<p><p>Myxoid liposarcoma (MLPS) has different patterns that are often difficult to distinguish from other soft tissue lesions. MLPS is characterized by a reciprocal translocation involving the DNA Damage Inducible Transcript 3 gene <i>(DDIT3)</i> that can be detected using fluorescent in situ hybridization (FISH). Recently, the marker for cancer testis antigen 1b (CTAG1B) was found to be expressed in MLPS. The aim of the present study was to assess the potential use immunohistochemistry (IHC) for CTAG1B expression and <i>DDIT3</i> rearrangement to diagnose MLPS and distinguish it from similar lesions. Out of 29 cases including MLPS and its mimics, CTAG1B was expressed in 92.86% of cases of MLPS and 20% of its mimics. <i>DDIT3</i> rearrangement was 100% sensitive and 92.86% specific in distinguishing MLPS from its mimics. The <i>DDIT3</i> rearrangement was found to be more sensitive but less specific than cytoplasmic expression of CTAG1B marker. <i>DDIT3</i> polysomy and amplification were detected in some cases. Therefore, both CTAG1B expression and FISH for <i>DDIT3</i> gene can be used to distinguish MLPS from similar tumors. The use of both immunohistochemistry for CTAG1B in addition to <i>DDIT3</i> gene rearrangement detection by FISH was more specific than using either of them alone. However, the <i>DDIT3</i> gene rearrangement alone was the most sensitive test for distinguishing MLPS from its mimics.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39677869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glyoxal fixation: an immunohistochemistry assay evaluation.","authors":"Sheila L Criswell,&nbsp;Savanna Altman,&nbsp;Chelsea Peeler,&nbsp;Taylor Drake,&nbsp;Cynthia Lazar,&nbsp;Samantha Douglas,&nbsp;Victoria DeJarnatt","doi":"10.1080/01478885.2021.1996125","DOIUrl":"https://doi.org/10.1080/01478885.2021.1996125","url":null,"abstract":"<p><p>Analysis of surgical pathology specimens by histological techniques including immunohistochemistry (IHC) assays is a mainstay of disease diagnosis in humans. Neutral buffered formalin (NBF) is currently the primary fixative used, but its use is not without risks due to toxicity and carcinogenicity. Several glyoxal-based fixatives have been commercially produced, are considered safer alternatives to NBF, and produce histochemical staining results comparable to that of tissues fixed in NBF. However, previous studies evaluating IHC assay results in tissues fixed in NBF and glyoxal solutions have indicated mixed results. This study demonstrated that while tissues fixed in NBF were slightly superior to tissues fixed in glyoxal solutions among the 34 antibodies assayed with IHC, all fixative solutions produced results compatible for use in an anatomic pathology laboratory.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39815136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Test Your Knowledge","authors":"Brooke H. Dubansky, Glenda F. Hood","doi":"10.1080/01478885.2022.2065734","DOIUrl":"https://doi.org/10.1080/01478885.2022.2065734","url":null,"abstract":"(2022). Test Your Knowledge. Journal of Histotechnology: Vol. 45, No. 2, pp. 93-93.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138504214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of two reprocessing methods for formalin fixed paraffin embedded tissue","authors":"Matthew D Lunetta, Megan Grivois, Christopher Hansen, Peter Seery, Cameron C Felty, Elizabeth Rizzo, Christopher R. Jackson","doi":"10.1080/01478885.2022.2062532","DOIUrl":"https://doi.org/10.1080/01478885.2022.2062532","url":null,"abstract":"ABSTRACT Formalin fixed paraffin embedded tissue occasionally requires reprocessing if the histologic quality of a section is inadequate for clinical diagnosis. The Pat Dry (PD) and the Serial Xylene (SX) methods are two techniques described in the literature to reprocess under-fixed and/or under-processed tissue samples. To date, no study has compared the effects of these methods on the histologic quality of tissue sections, cost, and turnaround times. In the present study, these two methods were evaluated on 129 tissue samples taken from 40 submitted clinical specimens, 3 blocks per sampled location. Before processing, sample Group 1 (Control) was cut at routine 3–5 mm thickness. Sample Groups 2 and 3 were cut at 10 mm to ensure the thicker tissues would be poorly processed. Histotechnicians performed a subjective evaluation of all the samples at the time of embedding and microtomy. Hematoxylin and eosin stained sections from all samples were scored for histologic quality by two pathology residents. Thicker samples (Groups 2 and 3) were then reprocessed using either PD or SX methods, re-sectioned, stained, and then re-scored by the pathology residents. The two reprocessing methods equally improved quality scores and reduced the fraction of slides that were rejected. The PD method average preparation time was 66 minutes as compared to 250 minutes for the SX method. The PD method was easier to perform than the SX method, required less reagent, and was less susceptible to reagent spillage than the SX method.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46977072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Journal of Histotechnology as an educational tool","authors":"G. Callis","doi":"10.1080/01478885.2022.2065733","DOIUrl":"https://doi.org/10.1080/01478885.2022.2065733","url":null,"abstract":"The Journal of Histotechnology is a readily available educational tool for histology professionals in clinical and research disciplines. One specific publication for National Society for Histotechnology (NSH) members is the Test Your Knowledge (TYK) article that is found in each journal issue. A NSH member can earn continuing education credits (CE) by taking the TYK quiz found on the NSH website at https://www.tandfonline. com/toc/yhis20/current. Unfortunately, this educational resource is not available to non-members, but they can still learn from it or use it as a means to teach students histology. Please read the current TYK in this and other JOH issues. In addition to TYK, JOH publications are another way to help teach students in histotechnology and further the knowledge of those already experienced in using histotechniques. The article by Lattouf, Assoumau-Abroh, and Younes et al. on inherited connective tissue diseases has excellent connective staining methods using picrosirius red and an elastic fiber stain called catechine-fuchsin along with a routine H&E. The elastic stain may not be one commonly known but is effective in demonstrating these fibers. Together, the three stains in this paper provided excellent comparisons of morphology and connective tissues for the inherited diseases of the skin. The cover image is from this publication. Glyoxal, a formalin substitute, was compared to neutral buffered formalin (NBF) and several glyoxal solutions for immunohistochemistry (IHC) assays by Criswell and colleagues. Since glyoxal is frequently considered as a replacement for NBF, histotechnicians will learn more about the results for IHC on glyoxal-fixed tissues. Laboratories are always seeking safe alternatives for reagents used to ‘buffer’ tissues from liquid nitrogen (LN2) temperature when snap freezing tissues. The Nunez group compared three commercial coolants at different cold temperatures and LN2 for the purpose of preserving RNA and tissue morphology in nine different tissues. Their images of frozen sections stained with H&E compare morphology results of each freezing reagent. You will learn which coolants provided the best morphology, are non-flammable and non-toxic along with helpful technical hints for cryomicrotomy. If your laboratory is considering something different than using LN2-cooled isopentane, then this paper provides alternatives for this method. The paper on an immunohistochemical assay and fluorescence in situ hybridization (FISH) for a gene rearrangement study to distinguish myxoid liposarcoma from its mimics by the Abdelaziz group provides readers with results for this disease. However, it is important to see the detailed information in methods and materials on how IHC and molecular assays were reported in order to authenticate reagents, antibodies, and even equipment used in order for others to reproduce these results. This is a nice lesson on how to maintain records especially for IHC and molecular assays particular","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59143309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}