Journal of Histotechnology最新文献_第8页

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-12-01 Epub Date: 2022-10-12 DOI: 10.1080/01478885.2022.2126110

Brooke H Dubansky, Glenda F Hood

引用次数: 0

A study on the safety and therapeutic effect of Xiaoyaosan on depressive disorder related dry eye disease in a murine animal model. 消药散对抑郁症相关性干眼病小鼠模型的安全性及疗效研究。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-12-01 Epub Date: 2022-10-31 DOI: 10.1080/01478885.2022.2136818

Jie Li, Ke Yan, Zhaolin Liu, Xiang Lin, Yu Huang, Jian Shi, Dongdong Li, Xiaolei Yao, Zuguo Liu, Qinghua Peng

{"title":"A study on the safety and therapeutic effect of Xiaoyaosan on depressive disorder related dry eye disease in a murine animal model.","authors":"Jie Li, Ke Yan, Zhaolin Liu, Xiang Lin, Yu Huang, Jian Shi, Dongdong Li, Xiaolei Yao, Zuguo Liu, Qinghua Peng","doi":"10.1080/01478885.2022.2136818","DOIUrl":"https://doi.org/10.1080/01478885.2022.2136818","url":null,"abstract":"This study was done to observe the safety and effect of Xiaoyaosan (XYS) on the stimulated depressive disorder (DD) related dry eye disease (DED) in mice. Normal control (NC) group, Vehicle group, and drug treatment groups, including Sertraline Hydrochloride (SH), XYS low-dose (XYS-LD), medium-dose (XYS-MD), and high-dose (XYS-HD), were established. The drug quality of XYS was assessed by high-performance liquid chromatography (HPLC). XYS toxicity in kidney and liver was assessed with Hematoxylin & Eosin (H&E) staining. Serum enzyme-linked immunosorbent assay (ELISA) and body weights were used to evaluate the depression status of mice. Tear production, corneal sensitivity, Oregon Green Dye (OGD) staining, and corneal confocal microscopy were used to assess ocular surface changes. H&E staining was also used to assess pathological cornea and lacrimal gland changes. HPLC results showed that XYS complied with Chinese drug quality standards. The drug treatment groups observed no drug toxicity reactions in the liver and kidney. SH and XYS groups improved DD-related serological indices as compared with Vehicle. Body weight was enhanced in mice with XYS groups compared to Vehicle and SH. Mice with XYS treatments showed increased tear production and corneal sensitivity, decreased corneal OGD staining scores, improved morphology, and decreased inflammatory cell infiltration in the cornea and lacrimal gland. In conclusion, XYS had no drug toxicity, improved serological indices, and ocular surface pathological changes in DD-related DED mice. XYS is safe and may have a therapeutic effect on DD-related DED.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"161-171"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40436560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Histopathology-based diagnosis of Mooren's ulcer concealed beneath the pterygium on eye. 隐匿于眼翼状胬肉下的莫伦氏溃疡的组织病理学诊断。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-12-01 Epub Date: 2022-11-11 DOI: 10.1080/01478885.2022.2137666

Yujie Zhang, Xie Fang, Zhirong Lin, Zhiwen Xie, Huping Wu, Shangkun Ou

{"title":"Histopathology-based diagnosis of Mooren's ulcer concealed beneath the pterygium on eye.","authors":"Yujie Zhang, Xie Fang, Zhirong Lin, Zhiwen Xie, Huping Wu, Shangkun Ou","doi":"10.1080/01478885.2022.2137666","DOIUrl":"https://doi.org/10.1080/01478885.2022.2137666","url":null,"abstract":"Mooren's ulcer (MU) is a chronic and painful ulcerative keratitis that is difficult to diagnose, especially when concealed beneath the pterygium, which is a common, benign, wedge-shaped, fleshy tissue growth of the conjunctiva extending onto the cornea. The coexistence of MU and pterygium is extremely rare. A 41-year-old man presented with a 2-month history of unprovoked redness, pain, and blurred vision in the right eye. Corneal epithelial defects around the pterygium head were noted upon slit-lamp examination and fluorescein staining. The patient was initially misdiagnosed with a corneal epithelial defect and pterygium. The initial treatments with anti-inflammatory and corneal epithelial growth promotion tear agents failed. Anterior segment optical coherence tomography (AS-OCT) showed corneal stromal lysis thinning, and in vivo confocal microscopy (IVCM) revealed marked inflammatory cell infiltration and stromal degeneration. We suspected the pathology was an immune-related or tumor-related corneal ulcer. The MU concealed beneath the pterygium was diagnosed by histopathological examination of a biopsy specimen that presented typical localized loss of the corneal epithelium and Bowman's layer, stromal degeneration, and inflammatory cell infiltration. Finally, we performed lamellar keratoplasty (LKP) combined with pterygium excision surgery. The patient recovered with no complications or recurrence during the 1-year follow-up period. Few cases of MU concealed beneath the pterygium have been reported. It is beneficial to rule out the pathological changes concealed beneath the pterygium, combined with multiple means of examination such as slit-lamp examination, AS-OCT, and IVCM. A histopathological examination should be performed to establish a diagnosis.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"195-201"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40472608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Test your knowledge. 测试你的知识。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-12-01 Epub Date: 2022-11-15 DOI: 10.1080/01478885.2022.2131063

Dale Telgenhoff

引用次数: 0

Guide for collecting and reporting metadata on protocol variables and parameters from slide-based histotechnology assays to enhance reproducibility. 从基于幻灯片的组织技术分析中收集和报告关于协议变量和参数的元数据的指南，以提高可重复性。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-12-01 Epub Date: 2022-11-01 DOI: 10.1080/01478885.2022.2134022

Luis Chiriboga, Gayle M Callis, Yongfu Wang, Elizabeth Chlipala

{"title":"Guide for collecting and reporting metadata on protocol variables and parameters from slide-based histotechnology assays to enhance reproducibility.","authors":"Luis Chiriboga, Gayle M Callis, Yongfu Wang, Elizabeth Chlipala","doi":"10.1080/01478885.2022.2134022","DOIUrl":"https://doi.org/10.1080/01478885.2022.2134022","url":null,"abstract":"The central tenet of scientific research is the rigorous application of the scientific method to experimental design, analysis, interpretation, and reporting of results. In order to confer validity to a hypothesis, experimental details must be transparent and results must be reproducible. Failure to achieve this minimum indicates a deficiency in rationale, design, and/or execution, necessitating further experimental refinement or hypothesis reformulation. More importantly, rigorous application of the scientific method advances scientific knowledge by enabling others to identify weaknesses or gaps that can be exploited by new ideas or technology that inevitably extend, improve, or refine a hypothesis. Experimental details, described in manuscript materials and methods, are the principal vehicle used to communicate procedures, techniques, and resources necessary for experimental reproducibility. Recent examination of the biomedical literature has shown that many published articles lack sufficiently detailed methodological information to reproduce experiments. There are few broadly established practice guidelines and quality assurance standards in basic biomedical research. The current paper provides a framework of best practices to address the lack of reporting of detailed materials and methods that is pervasive in histological slide-based assays. Our goal is to establish a structured framework that highlights the key factors necessary for thorough collection of metadata and reporting of slide-based assays.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"132-147"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40674378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The intervention of epithelial-mesenchymal transition in homeostasis of human retinal pigment epithelial cells: a review. 上皮-间质转化对人视网膜色素上皮细胞稳态的干预研究进展。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-12-01 Epub Date: 2022-11-15 DOI: 10.1080/01478885.2022.2137665

Brijesh Gelat, Pooja Rathaur, Pooja Malaviya, Binita Patel, Krupali Trivedi, Kaid Johar, Rahul Gelat

{"title":"The intervention of epithelial-mesenchymal transition in homeostasis of human retinal pigment epithelial cells: a review.","authors":"Brijesh Gelat, Pooja Rathaur, Pooja Malaviya, Binita Patel, Krupali Trivedi, Kaid Johar, Rahul Gelat","doi":"10.1080/01478885.2022.2137665","DOIUrl":"https://doi.org/10.1080/01478885.2022.2137665","url":null,"abstract":"The health and activity of photoreceptors and Bruch's membrane are promoted by the retinal pigment epithelium (RPE), which is essential for normal vision. Age-related macular degeneration (AMD), diabetic retinopathy (DR), and proliferative vitreoretinopathy (PVR) are examples of retinopathies that result in vision loss. Epithelial-mesenchymal transition (EMT) is a process in which epithelial cells transform into mesenchymal cells as a result of a faulty microenvironment, and it is associated with the oculopathies stated above. Cell differentiation, autophagy, growth factors (GFs), the blood-retinal barrier (BRB), and other complicated signaling pathways all contribute to proper morphology, and their disruption by harmful compounds has an impact on RPE function. The inducer and suppressor of EMT in RPE, on the other hand, are unknown. The current article reviews the experimental research investigations, suggested that certain modulators like glucosamine (Glc-N) and bradykinin (BK) suppress the TGFβ signaling pathway and that other variables like oxidative stress triggered EMT, which is not found in normal RPE homeostasis. Finding molecular targets and treatments to prevent and restore RPE function, as well as understanding how EMT regulators affect RPE degeneration, are therefore crucial.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"148-160"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40685880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A freeze-substitution approach with solvent-based glyoxal fixative to prevent distortion of ocular structures. 溶剂型乙二醛固定物的冷冻取代方法防止眼结构畸变。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-12-01 Epub Date: 2022-09-16 DOI: 10.1080/01478885.2022.2111744

Ji Pang, Xia Zhao, Fengyan Deng, Dai Tsuchiya, Seth Malloy, Tari Parmely, Ting Xie, Yongfu Wang

{"title":"A freeze-substitution approach with solvent-based glyoxal fixative to prevent distortion of ocular structures.","authors":"Ji Pang, Xia Zhao, Fengyan Deng, Dai Tsuchiya, Seth Malloy, Tari Parmely, Ting Xie, Yongfu Wang","doi":"10.1080/01478885.2022.2111744","DOIUrl":"https://doi.org/10.1080/01478885.2022.2111744","url":null,"abstract":"Investigating the function of delicate mammalian eyes often requires chemical fixation, histological sectioning, immunohistochemistry (IHC) and in situ hybridization (ISH). One of the long-standing challenges in the ocular histology field is the limited success of maintaining intact morphology via cryo- or paraffin procedures. Although our latest protocol significantly improved the morphology of mouse eyeball sections, the window technique is time-consuming and requires extensive practice to avoid damage while making windows. In this study, we present a novel glyoxal fixative that is suitable for a freeze-substitution approach to improve both morphology and molecular target preservation of mouse eyes. The method prevents morphology distortion in all tested eyeballs. Therefore, it suits a variety of research needs from morphological examination to investigation of single-molecule RNA expression, using hematoxylin and eosin (H&E) stain, IHC, and ISH assays on either frozen (cryo) or paraffin-infiltrated tissue sections. In addition, this method can be easily performed in many histology laboratories.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"172-181"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40362903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved handling and embedding schemes for cultured murine neuroretinal explants. 改进小鼠神经视网膜外植体培养的处理和包埋方案。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-10-12 DOI: 10.1080/01478885.2022.2119639

Susan Haller, Rebecca M Marton, Kevin A Marroquin, Eliah R Shamir

{"title":"Improved handling and embedding schemes for cultured murine neuroretinal explants.","authors":"Susan Haller, Rebecca M Marton, Kevin A Marroquin, Eliah R Shamir","doi":"10.1080/01478885.2022.2119639","DOIUrl":"10.1080/01478885.2022.2119639","url":null,"abstract":"Traumatic, inherited, and age-related degenerative diseases of the retina, such as retinal detachment, retinitis pigmentosa, and age-related macular degeneration, are characterized by the irreversible loss of retinal neurons. While current treatments aim to prevent neuronal degeneration, there are no available treatments to restore neurons after loss. Cultured murine neuroretinal tissue explants model retinal injury and offer a high throughput approach to identify experimental interventions capable of regenerating neurons. Formalin-fixed paraffin-embedded (FFPE) preparations of murine neuroretinal explants can be used to identify cells throughout the retinal layers to provide information on proliferation and activity following exposure to therapeutics. However, retinal explants are friable, particularly after ex vivo culture, sample handling and FFPE processing steps can result in tissue loss and damage. Friability also prohibits bisecting samples post-culture to display more than one region of interest for analysis. We developed a sample handling and embedding technique for cultured murine neuroretinal explants using HistogelTM in combination with a post-processing trimming step that eliminates tissue loss, increases cross-sectional retinal representation, and captures proximal and central retina on one slide to facilitate analysis of explants subjected to neurotrophic compounds.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":1.1,"publicationDate":"2022-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33500243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Color atlas of human gross pathology 人类大体病理彩色图谱

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-09-16 DOI: 10.1080/01478885.2022.2123126

S. Lau

引用次数: 0

A new method for the sampling and preservation of placental specimens in low-resource settings for the identification of P. falciparum and analysis of nucleic acids. 一种在低资源环境下采集和保存胎盘标本用于恶性疟原虫鉴定和核酸分析的新方法。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-09-01 DOI: 10.1080/01478885.2022.2088191

Patrick S Potoczak, Beverly I Strassmann, Claudius Vincenz

引用次数: 0