Journal of Histotechnology最新文献

{"title":"Test your knowledge.","authors":"Dale Telgenhoff","doi":"10.1080/01478885.2022.2131063","DOIUrl":"https://doi.org/10.1080/01478885.2022.2131063","url":null,"abstract":"( 1 ) I m m u n o c y t o c h e m i s t r y / i m m u n o fl u o r e s c e n c e a p p l i e d t o c e l l s i n c u l t u r e . ( 2 ) A c t i n i n t e r m e d i a t e fi l a m e n t s o f t h e c y t o s k e l e t o n ( 3 ) C e l l m i g r a t i o n , m o v e m e n t o f v e s i c l e s i n t h e c e l l , m e c h a n i c a l s u p p o r t , c e l l d i v i s i o n ( 4 ) T h i s c e l l i s v e r y a c t i v e a n d r e q u i r e s a l a r g e a m o u n t o f A T P t o f a c i l i t a t e i t s f u n c t i o n . I n t h i s c a s e , t h e A T P i s b e i n g g e n e r a t e d t o s u p p o r t c e l l m i g r a t i o n .","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"204-205"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40685387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guide for collecting and reporting metadata on protocol variables and parameters from slide-based histotechnology assays to enhance reproducibility.","authors":"Luis Chiriboga,&nbsp;Gayle M Callis,&nbsp;Yongfu Wang,&nbsp;Elizabeth Chlipala","doi":"10.1080/01478885.2022.2134022","DOIUrl":"https://doi.org/10.1080/01478885.2022.2134022","url":null,"abstract":"<p><p>The central tenet of scientific research is the rigorous application of the scientific method to experimental design, analysis, interpretation, and reporting of results. In order to confer validity to a hypothesis, experimental details must be transparent and results must be reproducible. Failure to achieve this minimum indicates a deficiency in rationale, design, and/or execution, necessitating further experimental refinement or hypothesis reformulation. More importantly, rigorous application of the scientific method advances scientific knowledge by enabling others to identify weaknesses or gaps that can be exploited by new ideas or technology that inevitably extend, improve, or refine a hypothesis. Experimental details, described in manuscript materials and methods, are the principal vehicle used to communicate procedures, techniques, and resources necessary for experimental reproducibility. Recent examination of the biomedical literature has shown that many published articles lack sufficiently detailed methodological information to reproduce experiments. There are few broadly established practice guidelines and quality assurance standards in basic biomedical research. The current paper provides a framework of best practices to address the lack of reporting of detailed materials and methods that is pervasive in histological slide-based assays. Our goal is to establish a structured framework that highlights the key factors necessary for thorough collection of metadata and reporting of slide-based assays.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"132-147"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40674378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The intervention of epithelial-mesenchymal transition in homeostasis of human retinal pigment epithelial cells: a review.","authors":"Brijesh Gelat,&nbsp;Pooja Rathaur,&nbsp;Pooja Malaviya,&nbsp;Binita Patel,&nbsp;Krupali Trivedi,&nbsp;Kaid Johar,&nbsp;Rahul Gelat","doi":"10.1080/01478885.2022.2137665","DOIUrl":"https://doi.org/10.1080/01478885.2022.2137665","url":null,"abstract":"<p><p>The health and activity of photoreceptors and Bruch's membrane are promoted by the retinal pigment epithelium (RPE), which is essential for normal vision. Age-related macular degeneration (AMD), diabetic retinopathy (DR), and proliferative vitreoretinopathy (PVR) are examples of retinopathies that result in vision loss. Epithelial-mesenchymal transition (EMT) is a process in which epithelial cells transform into mesenchymal cells as a result of a faulty microenvironment, and it is associated with the oculopathies stated above. Cell differentiation, autophagy, growth factors (GFs), the blood-retinal barrier (BRB), and other complicated signaling pathways all contribute to proper morphology, and their disruption by harmful compounds has an impact on RPE function. The inducer and suppressor of EMT in RPE, on the other hand, are unknown. The current article reviews the experimental research investigations, suggested that certain modulators like glucosamine (Glc-N) and bradykinin (BK) suppress the TGFβ signaling pathway and that other variables like oxidative stress triggered EMT, which is not found in normal RPE homeostasis. Finding molecular targets and treatments to prevent and restore RPE function, as well as understanding how EMT regulators affect RPE degeneration, are therefore crucial.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"148-160"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40685880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A freeze-substitution approach with solvent-based glyoxal fixative to prevent distortion of ocular structures.","authors":"Ji Pang,&nbsp;Xia Zhao,&nbsp;Fengyan Deng,&nbsp;Dai Tsuchiya,&nbsp;Seth Malloy,&nbsp;Tari Parmely,&nbsp;Ting Xie,&nbsp;Yongfu Wang","doi":"10.1080/01478885.2022.2111744","DOIUrl":"https://doi.org/10.1080/01478885.2022.2111744","url":null,"abstract":"<p><p>Investigating the function of delicate mammalian eyes often requires chemical fixation, histological sectioning, immunohistochemistry (IHC) and <i>in situ</i> hybridization (ISH). One of the long-standing challenges in the ocular histology field is the limited success of maintaining intact morphology via cryo- or paraffin procedures. Although our latest protocol significantly improved the morphology of mouse eyeball sections, the window technique is time-consuming and requires extensive practice to avoid damage while making windows. In this study, we present a novel glyoxal fixative that is suitable for a freeze-substitution approach to improve both morphology and molecular target preservation of mouse eyes. The method prevents morphology distortion in all tested eyeballs. Therefore, it suits a variety of research needs from morphological examination to investigation of single-molecule RNA expression, using hematoxylin and eosin (H&E) stain, IHC, and ISH assays on either frozen (cryo) or paraffin-infiltrated tissue sections. In addition, this method can be easily performed in many histology laboratories.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"172-181"},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40362903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved handling and embedding schemes for cultured murine neuroretinal explants.","authors":"Susan Haller, Rebecca M Marton, Kevin A Marroquin, Eliah R Shamir","doi":"10.1080/01478885.2022.2119639","DOIUrl":"10.1080/01478885.2022.2119639","url":null,"abstract":"<p><p>Traumatic, inherited, and age-related degenerative diseases of the retina, such as retinal detachment, retinitis pigmentosa, and age-related macular degeneration, are characterized by the irreversible loss of retinal neurons. While current treatments aim to prevent neuronal degeneration, there are no available treatments to restore neurons after loss. Cultured murine neuroretinal tissue explants model retinal injury and offer a high throughput approach to identify experimental interventions capable of regenerating neurons. Formalin-fixed paraffin-embedded (FFPE) preparations of murine neuroretinal explants can be used to identify cells throughout the retinal layers to provide information on proliferation and activity following exposure to therapeutics. However, retinal explants are friable, particularly after <i>ex vivo</i> culture, sample handling and FFPE processing steps can result in tissue loss and damage. Friability also prohibits bisecting samples post-culture to display more than one region of interest for analysis. We developed a sample handling and embedding technique for cultured murine neuroretinal explants using Histogel^TM in combination with a post-processing trimming step that eliminates tissue loss, increases cross-sectional retinal representation, and captures proximal and central retina on one slide to facilitate analysis of explants subjected to neurotrophic compounds.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":1.1,"publicationDate":"2022-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33500243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Color atlas of human gross pathology","authors":"S. Lau","doi":"10.1080/01478885.2022.2123126","DOIUrl":"https://doi.org/10.1080/01478885.2022.2123126","url":null,"abstract":"","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"45 1","pages":"202 - 202"},"PeriodicalIF":1.1,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48794766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new method for the sampling and preservation of placental specimens in low-resource settings for the identification of <i>P. falciparum</i> and analysis of nucleic acids.","authors":"Patrick S Potoczak,&nbsp;Beverly I Strassmann,&nbsp;Claudius Vincenz","doi":"10.1080/01478885.2022.2088191","DOIUrl":"https://doi.org/10.1080/01478885.2022.2088191","url":null,"abstract":"<p><p>Collection, preservation, and shipment of histological specimens in low-resource settings is challenging. We present a novel method that achieved excellent preservation of placental specimens from rural Mali by using formalin fixation, ethanol dehydration, and long-term storage in a solar-powered freezer. Sample preservation success was 92%, permitting evaluation of current and past malaria infection, anemia, placental maturity, and inflammation. Using RNAscope® hybridization we were able to visualize cell-specific gene expression patterns in the formalin-fixed paraffin-embedded (FFPE) specimens. Additionally, our method entailed mirrored sampling from the two cut faces of a cotyledon, one for the FFPE workflows and the other for storage in RNAlater™ and RNA-seq.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"45 3","pages":"116-119"},"PeriodicalIF":1.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9437128/pdf/nihms-1821218.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10505111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the gap in the Occupational Safety And Health Administration (OSHA) laboratory standard: a literature review and recommendations to enhance histology laboratory safety practices.","authors":"Jeana Earn","doi":"10.1080/01478885.2022.2099720","DOIUrl":"https://doi.org/10.1080/01478885.2022.2099720","url":null,"abstract":"<p><p>This article discusses current available resources with respect to regulatory agencies including the Occupational Safety and Health Administration (OSHA) for determining the requirements placed upon laboratories for handling of hazardous materials. The focus is specific to the histology laboratory and xylene use, and includes a literature review, admixed with historical reference points. Procedures and tasks in the histology laboratory are highlighted in relation to their connection to the quality of the work environment with an emphasis on air quality. Recommendations are provided for maintaining an appropriate work environment for the prevention of potential adverse health effects. The gap within the OSHA Laboratory Standard, i.e. a lack of explanatory language, leaves much open to interpretation regarding fume hood usage with volatile hazardous chemicals. As a result, both the level of safety training and the awareness of good laboratory practices (GLP) for handling volatile hazardous reagents such as xylene can become compromised.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"107-115"},"PeriodicalIF":1.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40608975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Journal of Histotechnology 2022 NSH convention issue.","authors":"Gayle M Callis","doi":"10.1080/01478885.2022.2101238","DOIUrl":"https://doi.org/10.1080/01478885.2022.2101238","url":null,"abstract":"The National Society for Histotechology has always had a convention issue as a hand out to attendees and for NSH members to pick up extra copies. It is nice to know this will happen in 2022 after the 2020 and 2021 virtual conventions. 2022 has been a productive year for JOH with good news that the 2021 Impact Factor is 1.918 and has more than doubled from 0.714 in 2020. This is a good indicator that JOH is doing very well and being recognized with more citations. It no doubt was helped by the fact JOH is now on Pubmed/Medline. For all of those who submitted and now have publications in JOH, you have my heartiest thank you for making the journal more successful than ever in the scientific and medical communities. In my thanks, I also include our peer reviewers and the JOH Editorial Board members. If you want to learn more about journal metrics, go to the JOH website https://www.tandfonline.com/action/ journalInformation and read why metrics is an important tool for for both readers and authors. This issue has articles which deal with three practical issues so often found in an everyday working histology laboratory. Questions concerning cell block preparation are frequently seen on The Block. This is addressed by Clif Chapman in his paper showing an ‘innovative’ standardized method for a cell block procedure and preparation, processing and staining. This paper is well illustrated with photographs and images of sections from these cell blocks. One problem that confronts histotechnicians, often on a daily basis, is poor paraffin processing that requires using a ‘reprocessing’ method to recover valuable tissue in order to have a proper diagnosis. Lunetta and colleagues did a careful study to recreate poor processing and then comparing two reprocessing methods commonly used by laboratories. This is a nice review of the two known reprocessing methods but more important was the simulation of poor processing then performing the reprocessing with the two methods. The evaluation and comparison of the two methods at microtomy and with staining is well documented along with photomicrographs of stained tissue sections both before and after reprocessing. This paper can provide guidelines and aid in choosing a reprocessing method for any histology laboratory. Jeana Earn provided a paper exploring the gap in the OSHA laboratory standard using a literature review along with recommendations to enhance laboratory safety practice. The topic is specific for volatile hazardous chemicals with emphasis on xylene, the importance of good laboratory practices (GLP) and the being familiar with agencies that provide guidelines. Although many histology personnel are already familiar with the OSHA standard, this is a good review to remind laboratory personnel that good safety practices in the histology laboratory are necessary to protect the health of workers especially histotechnicians. The paper on sampling of placentas to diagnose Plasmodium falciparium, the parasite that ca","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"95"},"PeriodicalIF":1.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40518098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Test Your Knowledge.","authors":"Brooke H Dubansky","doi":"10.1080/01478885.2022.2101242","DOIUrl":"https://doi.org/10.1080/01478885.2022.2101242","url":null,"abstract":"","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"130"},"PeriodicalIF":1.1,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40613966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}