Journal of Histotechnology最新文献_第7页

Practical gynecologic pathology: frequently asked questions 妇科实用病理学：常见问题

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-01-02 DOI: 10.1007/978-3-030-68608-6

S. Lau

引用次数: 0

Practical gynecologic pathology: frequently asked questions 实用妇科病理学:常见问题

4区生物学

Journal of Histotechnology Pub Date : 2023-01-02 DOI: 10.1080/01478885.2023.2165726

Stephen K. Lau

引用次数: 0

Test Your Knowledge. 测试你的知识。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-12-01 Epub Date: 2022-10-12 DOI: 10.1080/01478885.2022.2126110

Brooke H Dubansky, Glenda F Hood

引用次数: 0

Improved handling and embedding schemes for cultured murine neuroretinal explants. 改进小鼠神经视网膜外植体培养的处理和包埋方案。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-10-12 DOI: 10.1080/01478885.2022.2119639

Susan Haller, Rebecca M Marton, Kevin A Marroquin, Eliah R Shamir

{"title":"Improved handling and embedding schemes for cultured murine neuroretinal explants.","authors":"Susan Haller, Rebecca M Marton, Kevin A Marroquin, Eliah R Shamir","doi":"10.1080/01478885.2022.2119639","DOIUrl":"10.1080/01478885.2022.2119639","url":null,"abstract":"Traumatic, inherited, and age-related degenerative diseases of the retina, such as retinal detachment, retinitis pigmentosa, and age-related macular degeneration, are characterized by the irreversible loss of retinal neurons. While current treatments aim to prevent neuronal degeneration, there are no available treatments to restore neurons after loss. Cultured murine neuroretinal tissue explants model retinal injury and offer a high throughput approach to identify experimental interventions capable of regenerating neurons. Formalin-fixed paraffin-embedded (FFPE) preparations of murine neuroretinal explants can be used to identify cells throughout the retinal layers to provide information on proliferation and activity following exposure to therapeutics. However, retinal explants are friable, particularly after ex vivo culture, sample handling and FFPE processing steps can result in tissue loss and damage. Friability also prohibits bisecting samples post-culture to display more than one region of interest for analysis. We developed a sample handling and embedding technique for cultured murine neuroretinal explants using HistogelTM in combination with a post-processing trimming step that eliminates tissue loss, increases cross-sectional retinal representation, and captures proximal and central retina on one slide to facilitate analysis of explants subjected to neurotrophic compounds.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"1-13"},"PeriodicalIF":1.1,"publicationDate":"2022-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33500243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Color atlas of human gross pathology 人类大体病理彩色图谱

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-09-16 DOI: 10.1080/01478885.2022.2123126

S. Lau

引用次数: 0

A new method for the sampling and preservation of placental specimens in low-resource settings for the identification of P. falciparum and analysis of nucleic acids. 一种在低资源环境下采集和保存胎盘标本用于恶性疟原虫鉴定和核酸分析的新方法。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-09-01 DOI: 10.1080/01478885.2022.2088191

Patrick S Potoczak, Beverly I Strassmann, Claudius Vincenz

引用次数: 0

Serum glucose, sodium and potassium concentrations in patients with diabetic ketoacidosis. 糖尿病酮症酸中毒患者的血清葡萄糖、钠和钾浓度。

4区生物学

Journal of Histotechnology Pub Date : 2022-08-01 DOI: 10.17392/1471-22

Željka Dragila, Dora Rudić, Klara Ćosić, Tatjana Bačun

{"title":"Serum glucose, sodium and potassium concentrations in patients with diabetic ketoacidosis.","authors":"Željka Dragila, Dora Rudić, Klara Ćosić, Tatjana Bačun","doi":"10.17392/1471-22","DOIUrl":"10.17392/1471-22","url":null,"abstract":"Aim To investigate possible differences in serum glucose and sodium and potassium concentrations with respect to age, gender and severity of diabetic ketoacidosis. Methods Medical records from 1 January 2017 to 31 December 2019 were reviewed and patients with the diagnosis of diabetic ketoacidosis were selected. Results The study included 52 patients. Glucose concentration was significantly higher in the age group of 25-44 and >65 years compared to the group of 18-24 years (p=0.02). Sodium concentration was significantly higher in the age group 18-24 and >65 years compared to groups 25-44 and 45-65 years (p=0.002). Females had significantly higher sodium concentration than males (p=0.002). Potassium concentration was significantly higher in the age group 25-44 years compared to other groups (p=0.01). Males had significantly higher potassium concentration (p =0.01). Conclusion This study showed that significant differences exist in electrolyte concentration between specific age groups, male and female gender as well as DKA severity. Knowing these differences could help clinicians to promptly recognize and treat electrolyte derangements, leading to better outcome of patients with DKA.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87607375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surgical Pathology Review 外科病理学评论

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-07-03 DOI: 10.1080/01478885.2022.2097610

S. Lau

引用次数: 0

Tissue cryopreservation using the 3M™ Novec™ 7000 freezing coolant offers a comparable and safe alternative to customary coolants. 使用3M™Novec™7000冷冻冷却剂进行组织冷冻保存，提供了一种与传统冷却剂相当且安全的替代品。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-06-01 Epub Date: 2021-11-17 DOI: 10.1080/01478885.2021.1993528

Victor Nunez, Ashley Cook, Charles Havnar, Sean Flanagan, Nianfeng Ge, Angela Martzall, Robin E Taylor, Millicent Lu, Oleg Mayba, Oded Foreman

{"title":"Tissue cryopreservation using the 3M™ Novec™ 7000 freezing coolant offers a comparable and safe alternative to customary coolants.","authors":"Victor Nunez, Ashley Cook, Charles Havnar, Sean Flanagan, Nianfeng Ge, Angela Martzall, Robin E Taylor, Millicent Lu, Oleg Mayba, Oded Foreman","doi":"10.1080/01478885.2021.1993528","DOIUrl":"https://doi.org/10.1080/01478885.2021.1993528","url":null,"abstract":"Cryopreserving tissues for histology requires the use of coolants to buffer the sample from liquid nitrogen (LN2) and to control the rate of temperature decline. Several coolants sharing similar physical characteristics are available on the market; however, commonly used coolants are variably flammable and/or toxic and pose risks to personnel and facilities. The purpose of this study was to compare the performance of three commercially available coolants: hexane, 2-methylbutane (2 M), and 1-methoxyheptafluoropropane (N7000). Fresh mouse tissues were frozen by each method, for their ability to preserve microscopic architecture and to protect RNA from degradation were evaluated and compared to tissue characteristics obtained by direct immersion in LN2. Our results show that for most tissues, the N7000 freezing coolant provides equal or improved preservation of microscopic architecture. While snap-freezing tissues in LN2 provides superior RNA protection, no significant differences in RNA quality were seen between tissues frozen in hexane, 2 M, and N7000.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"45 2","pages":"85-91"},"PeriodicalIF":1.1,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39631158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inherited connective tissue diseases highlight macromolecular network interdependences in skin extracellular matrix: a histomorphometric study. 遗传性结缔组织疾病突出皮肤细胞外基质大分子网络相互依赖:一项组织形态计量学研究。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-06-01 Epub Date: 2022-02-09 DOI: 10.1080/01478885.2021.2024980

Raed Lattouf, Antoine Assoumou-Abroh, Ronald Younes, Didier Lutomski, Joseph Bassil, Claudine Blanchet-Bardon, Nada Naaman, Sylvie Changotade, Gaston Godeau, Karim Senni

{"title":"Inherited connective tissue diseases highlight macromolecular network interdependences in skin extracellular matrix: a histomorphometric study.","authors":"Raed Lattouf, Antoine Assoumou-Abroh, Ronald Younes, Didier Lutomski, Joseph Bassil, Claudine Blanchet-Bardon, Nada Naaman, Sylvie Changotade, Gaston Godeau, Karim Senni","doi":"10.1080/01478885.2021.2024980","DOIUrl":"https://doi.org/10.1080/01478885.2021.2024980","url":null,"abstract":"Mutation of just a single extracellular matrix protein, a receptor or enzyme involved in connective tissue metabolism is sufficient to cause systemic pathologies and failure of tissues that are subjected to strong mechanical stresses. Skin histological and computerized image analyses can provide a good qualitative and quantitative indication of these inherited connective tissue diseases. In this study, skin biopsies from young (10 to 25 years) and middle-aged patients (26 to 50 years) suffering from Ehlers-Danlos syndromes (EDS), Marfan syndrome (MS) or pseudoxanthoma elasticum (PXE) were studied after specific staining of both the collagen and elastic networks. Findings from the histomorphometric analyses conducted on skin sections of the patients with EDS, MS and PXE were compared to skin sections of healthy subjects from the same age groups. Our results show that both the collagen and the elastic networks were affected in all the studied pathological cases, but that the adverse changes to the elastic network in older patients were distinct from the physiological changes observed during aging process for healthy subjects. This degenerative process may be explained by an added phenomenon involving a general connective tissue proteolysis.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"45 2","pages":"66-76"},"PeriodicalIF":1.1,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39900644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0