Journal of Histotechnology最新文献

{"title":"The protective effect of erythropoietin on ischemia- reperfusion injury caused by ovarian torsion-detorsion in the experimental rat model.","authors":"Bahar Kartal,&nbsp;Mehmet Fatih Bozkurt,&nbsp;Ebru Alimoğullari,&nbsp;Uygar Saçık","doi":"10.1080/01478885.2022.2122653","DOIUrl":"https://doi.org/10.1080/01478885.2022.2122653","url":null,"abstract":"<p><p>Ovarian torsion is one of the most dangerous gynecological emergencies requiring surgery. A total of 50%-90% ovarian torsion cases are caused by physiological cysts, endometriosis, and other benign or malignant ovarian neoplasms. The aim of the study was to investigate the effects of erythropoietin (EPO) treatment on ischemia/reperfusion (IR) injury caused by ovarian torsion/detorsion (T/D) injury. Thirty female Wistar albino rats were divided into five groups as follows: Group I: Control; Group II: Torsion (T); Group III: Torsion/Detorsion(T/D); Group IV: Torsion/Detorsion (T/D) + EPO; Group V: EPO. Sections of the ovaries were evaluated for histopathological changes with hematoxylin and eosin stain, a immunohistochemical assay for caspase 3 expression, and the TUNEL assay for apoptosis. Ovarian sections from torsion/detorsion and torsion groups showed more hemorrhage, vascular congestion, edema, degenerative granulosa, and stromal cells. Fewer histopathological changes were found in EPO and T/D + EPO groups. Caspase 3 and TUNEL positive cells were significantly increased in the torsion/detorsion group as compared with the other groups (<i>p</i> < 0.05). Treatment with erythropoietin decreased the number of caspase 3 and TUNEL positive cells. The results of the study showed that erythropoietin administration is effective for recovery from degenerative changes in the ovary induced by the torsion-detorsion injury.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 2","pages":"57-64"},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9712663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epithelial ovarian carcinoma - a perspective.","authors":"Pranav S Renavikar,&nbsp;Jie Chen,&nbsp;Subodh M Lele","doi":"10.1080/01478885.2023.2204609","DOIUrl":"https://doi.org/10.1080/01478885.2023.2204609","url":null,"abstract":"The subtypes of epithelial ovarian carcinoma have differing pathophysiology, behavior, molecular alterations, and treatment approaches. As one of the most common malignancies diagnosed in females, ovarian carcinoma has the highest case-to-fatality ratio, primarily due to serous carcinoma presenting at an advanced stage with wide peritoneal metastasis and occasional lymph node metastasis. In comparison, the non-serous carcinomas, which most commonly include endometrioid and clear cell carcinomas, are frequently diagnosed at an early stage. Within serous carcinoma, low-grade and high-grade serous carcinoma are two distinct tumor types. Lowgrade serous carcinoma is thought to arise from precursor lesions like serous borderline tumor. In contrast, high-grade serous carcinoma is not related to a precursor lesion and thought to arise directly from malignant transformation of the ovarian surface epithelium (incessant ovulation theory) or from the epithelium of cortical inclusion cysts (resulting from exfoliated tubal epithelium implanting on the ovarian surface during ovulation). Recently, studies have shown that many high-grade serous carcinomas arise from the tubal fimbria from serous tubal intraepithelial carcinoma, particularly in BRCA-positive patients. BRAF, KRAS, and ERBB2 mutations are important molecular events in low-grade serous carcinoma while high-grade serous carcinoma is almost always associated with a TP53 mutation. Primary ovarian mucinous carcinoma is uncommon and needs workup to exclude metastasis from a gastrointestinal source. Like low-grade serous carcinoma, mucinous carcinoma is shown to arise from the adenoma-carcinoma sequence involving mucinous cystadenoma and mucinous borderline tumor, often coexisting in the same neoplasm. A high percentage of mucinous ovarian carcinomas have KRAS mutations. Most ovarian endometrioid adenocarcinomas are usually low-grade, unilateral and early-stage. They are often associated with endometriosis, endometriotic cyst, or endometrioid borderline tumor. Concurrently, the uterus may demonstrate atypical hyperplasia or endometrioid adenocarcinoma, as molecular events including PTEN, CTNNB1, KRAS and PIK3A mutations are shared between endometrioid adenocarcinoma of the two organs. Primary ovarian clear cell carcinoma occurs with a similar frequency, and shares some features with endometrioid adenocarcinoma, including early stage presentation and association with endometriosis. Recent studies have identified ARID1A mutations in most of these tumors [1].","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 2","pages":"55-56"},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10015803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of mTOR signal pathway in MMTV-TGFα mice ovary at different ages.","authors":"T Onel,&nbsp;E Yıldırım,&nbsp;S Dogan,&nbsp;A Yaba","doi":"10.1080/01478885.2022.2109883","DOIUrl":"https://doi.org/10.1080/01478885.2022.2109883","url":null,"abstract":"<p><p>Transforming growth factor alpha (TGFα), a member of the epidermal growth factor (EGF) family, regulates cell proliferation, differentiation, and development, and involves follicular development and viability. In ovaries, TGFα is shown localized in granulosa cells (GCs) of primary follicles, theca cells (TCs) of pre-antral, antral and pre-ovulatory follicles. TGFα overexpression in mouse mammary tumor virus (MMTV-TGFα) transgenic mice causes mammary tumor after 50 weeks. However, follicular development and preservation of the ovarian follicle reserve-mediating follicle stimulating hormone (FSH) response are unknown. Mammalian target of rapamycin (mTOR) is a key regulator for cell proliferation, growth, differentiation, and apoptosis, and important for ovarian folliculogenesis and oocyte maturation. The study aim determines TGFα overexpression during folliculogenesis via mTOR signaling pathway in ovaries from 10-, 18-, 50-, and 82-week-old MMTV-TGFα mice. Histological analysis was performed, along with western blot for mTOR, p-mTOR, P70S6K, PCNA, and Caspase-3, and quantitative RNA (qRT-PCR) for <i>mTOR</i> and <i>P70S6K</i>. Developing follicles number decreased and atretic follicles number increased with aging in MMTV-TGFα mice ovary. Ovaries at 18 and 82 weeks had decreased PCNA and increased Caspase-3 protein expression levels as compared to 10-week ovaries. Protein expression levels of mTOR and p-mTOR decreased gradually from ovaries at 10-18 weeks, increased at 50 weeks and decreased again at 82 weeks. These results indicate that TGFα may be one regulator of healthy follicular development and affect <i>mTOR</i> signaling pathway during ovarian aging. Thus, over-expression of TGFα might lead to reduced ovarian reserve and premature ovarian insufficiency.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 2","pages":"80-89"},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10014202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulatory effects of concomitant quercetin/sitagliptin administration on the ovarian histological and biochemical alterations provoked by doxorubicin in a streptozotocin-induced diabetic rat model.","authors":"Ahmed A Morsi,&nbsp;Eman Mohamed Faruk,&nbsp;Engy Medhat,&nbsp;Neama M Taha,&nbsp;Usama Fouad Ahmed Ebrahim","doi":"10.1080/01478885.2022.2105481","DOIUrl":"https://doi.org/10.1080/01478885.2022.2105481","url":null,"abstract":"<p><p>Limited literature was available on the effects of sitagliptin or quercetin treatments on doxorubicin induced ovarian dysfunction in diabetic animals. The study aim was test the efficacy and suggested mechanisms of quercetin/sitagliptin combined treatment on the doxorubicin-induced ovarian toxicity in rat model with streptozotocin-induced diabetes. Forty eight female Wistar rats were divided into six groups: 1) Control; 2) Streptozotocin induced diabetes; 3) Streptozotocin-induced diabetes + doxorubicin ovarian damage; 4) Streptozotocin-induced diabetes + doxorubicin ovarian damage with; 5) Streptozotocin-induced diabetes + doxorubicin ovarian damage with sitagliptin treatment and 6) Streptozotocin-induced diabetes + doxorubicin ovarian damage with concomitant quercetin/sitagliptin treatment. Biochemical tests for serum estrogen, progesterone, insulin, blood glucose, and ovarian levels of malondialdehyde, nitric oxide, and superoxide dismutase and qRT-PCR for NOBOX, FSHr, and iNOS genes were performed. Histological evaluation was done on ovary sections with hematoxylin and eosin and immunohistochemistry for 8-OHdG and iNOS followed by morphometric analysis. The streptozotocin-induced diabetic group showed varying degrees of follicle atresia and altered biochemical parameters, both were marked in the streptozotocin-induced diabetic + doxorubicin group. The mRNA of NOBOX, FSHr, and iNOS genes were disturbed with increased immunoexpression of iNOS and 8-OHdG. Quercetin and/or sitagliptin administration improved all altered histological and biochemical parameters and was more effective as a combined treatment. The study suggested equal efficacy of both quercetin and sitagliptin in mitigating the doxorubicin-induced ovarian toxicity in the streptozotocin diabetic rat model, and the combined therapy showed anti-inflammatory, anti-antioxidant, and anti-DNA damage mechanisms.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 2","pages":"65-79"},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9656197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The placenta: basics and clinical significance","authors":"S. Lau","doi":"10.1080/01478885.2023.2204610","DOIUrl":"https://doi.org/10.1080/01478885.2023.2204610","url":null,"abstract":"The placenta is a vital yet poorly understood organ and given the increased interest in recent years in its role in human development and the birth process, histologic examinations are increasingly being performed and cause some consternation to both pathologists and histology laboratories. Different aspects of the placenta are covered including chapters on the normal histology and physiology, immunology, endocrinology, and imaging characteristics of the placenta. [Extracted from the article] Copyright of Journal of Histotechnology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 1","pages":"96 - 96"},"PeriodicalIF":1.1,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49411653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical characterization of stem cell, vascular, neural, and differentiation markers in the apical papilla and dental pulp of human teeth at various stages of root development.","authors":"Cristina Retana-Lobo,&nbsp;Jessie Reyes-Carmona","doi":"10.1080/01478885.2022.2122665","DOIUrl":"https://doi.org/10.1080/01478885.2022.2122665","url":null,"abstract":"<p><p>This study aimed to evaluate the expression of several differentiation markers in the apical papilla (AP) and dental pulp (DP) of human permanent teeth. Twenty young human teeth were extracted and classified according to three Moorrees tooth development stages: initial root formation (Ri), root length ½ (R1/2), and root length complete (Rc). Immunohistochemical assays were performed using STRO-1, VEGF Receptor-2, Neurofilament heavy (NFH), and Nestin antibodies and analyzed under light microscopy. Decalcified, formalin fixed paraffin embedded tooth sections stained with hematoxylin and eosin showed an apical cell rich zone between the DP and AP. The AP revealed fewer vascular and cellular components than the DP. STRO-1 was expressed on vascular and neuronal elements beneath the odontoblast (OB) and in the sub-odontoblastic (SOB) zone, and VEGFR-2 positive cells were observed in the endothelium, arterioles, and blood vessels. Neuroepithelial stem cell protein (Nestin) was highly expressed in differentiated odontoblasts in the predentin odontotoblast and odontoblast cell processes. Neurofilament heavy (NFH) was expressed in mature axons throughout the DP. STRO-1 and VEGFR-2 microvascular expression was higher at the stages Ri and R1/2 while STRO-1 and NFH expression showed strong spatial distribution of Rc neuronal elements as compared to Ri and R1/2. Differentiated OB and SOB cells showed Nestin expression, indicating a reservoir of newly differentiated odontoblast-like cells.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 1","pages":"17-27"},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10840443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Attenuation of diethylnitrosamine-induced hepatocellular carcinoma in a rat model by combination therapy of diacerein and gold nanoparticles: a histopathological and immunohistochemical study.","authors":"Tourki A S Baokbah","doi":"10.1080/01478885.2022.2129935","DOIUrl":"https://doi.org/10.1080/01478885.2022.2129935","url":null,"abstract":"<p><p>The purpose of this study was to investigate the effect of combined therapy of diacerein and gold nanoparticles (AuNP) on diethylnitrosamine (DEN) induced hepatocellular carcinoma (HCC) in a rat model. Normal healthy and DEN-induced (HCC) rats were divided into five groups. Group I healthy rats served as normal control, Group II untreated HCC rats, Group III HCC rats administered diacerein, Group IV HCC rats administered AuNP, and Group V HCC rats administered diacerein and AuNP. All treatments were given once daily for 4 weeks. Liver morphology and necroinflammation in all groups were evaluated using hematoxylin and eosin (H&E), Masson's trichrome for fibrosis, and immunohistochemistry assays for expression of TNF-α, IL-6, β-catenin, and caspase-3. Liver sections from Group II HCC rats showed loss of lobular architecture, thick fibrous tissue deposition, leukocyte infiltration, degenerated hepatocytes and HCC neoplastic nodules surrounded by extensive fibrosis. Group II had high expression of TNF-α, IL-6, and β-catenin, and low caspase-3 expression as compared to Group I. HCC rats treated with the combined therapy of diacerein and AuNP (Group V) showed markedly decreased HCC lesions, significant necroinflammation reduction (p ˂ 0.05) and 90% reduction in fibrosis as compared to Group II HCC + diacerein. This combined therapy also reduced (p ˂ 0.05) TNF-α, IL-6, β-catenin expression and increased caspase-3 expression. In conclusion, diacerein combined with AuNP synergistically attenuated the severity of HCC lesions by reducing necroinflammation and fibrosis, decreased TNF-α, IL-6, β-catenin expression, and increased caspase-3 expression for apoptosis.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 1","pages":"5-16"},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9095767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological changes in the digestive tract of the Chinese soft-shelled turtle (<i>Pelodiscus sinensis</i>) during embryonic development.","authors":"Haili Zhang,&nbsp;Hongsong Wu,&nbsp;Wen Lu,&nbsp;Yanli Chang,&nbsp;Chunhua Li,&nbsp;Dechang Chu,&nbsp;Yan Chen,&nbsp;Xue Han,&nbsp;Na Li","doi":"10.1080/01478885.2022.2105490","DOIUrl":"https://doi.org/10.1080/01478885.2022.2105490","url":null,"abstract":"<p><p>The digestive tract development of the <i>Pelodiscus sinensis</i> embryo is described through the observation of the embryonic morphology on hematoxylin and eosin stained tissue sections. During the first 9 days of embryonic development, the anterior intestine of the embryo divides into the oral cavity, pharyngeal cavity, esophagus, stomach, and small intestine, while the caudal intestine differentiates into the cloaca, the anterior and caudal tubes of the large intestine. Between days 10-24, the wall of the digestive tract forms a two-layer structure consisting of mucosa and submucosa. The endoderm evolves into epithelial tissue in each part of the digestive tract, the mesoderm goes from a dense cluster of cells to looser mesenchymal tissue then divides into loose connective tissue, mesothelium, and muscle tissue. There is no clear temporal boundary between development of mesenchymal tissue and the early loose connective tissue, which is a gradual process.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 1","pages":"28-38"},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9406491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ulinastatin inhibits microglia activation in spinal cord via P2Y12 receptor in a rat neuropathic pain model.","authors":"Ying Shi,&nbsp;Huizhong Wen,&nbsp;Jian Cui,&nbsp;Wanxiang Qin","doi":"10.1080/01478885.2022.2163792","DOIUrl":"https://doi.org/10.1080/01478885.2022.2163792","url":null,"abstract":"<p><p>Ulinastatin, a broad spectrum of serine protease inhibitor, has been found to alleviate neuropathic pain (NPP). However, its mechanism is not completely clear. Here, a sciatic nerve ligation rat model and BV2 microglial cells were used to investigate the effect of Ulinastatin on the activation of microglia and P2Y12 receptors in vivo and in vitro. Levels of P2Y12 receptor and NF-κB (P65) expression in the dorsal horn of the lumbar enlargement region of the spinal cord and BV2 cells were assessed by immunohistochemistry and double-label immunofluorescence assays. Levels of IL-1β and TNF-α in cell culture medium and cerebrospinal fluid (CSF) were examined by ELISA. The results showed that Ulinastatin reduced the release of inflammatory IL-1β and TNF-α by inhibiting the activation of spinal microglia. Ulinastatin down-regulated P2Y12 receptor and NF-κB (P65) expression in the spinal microglia of the chronic constrictive injury model. The results indicated that Ulinastatin may attenuate the activation of spinal microglia after peripheral nerve injury by inhibiting the activation of P2Y12 receptor signal pathway in microglia. NF-kB may play a key role in the mechanism of Ulinastatin.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 1","pages":"39-53"},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9096747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell culture: <i>in vitro</i> model system and a promising path to <i>in vivo</i> applications.","authors":"Chongbei Zhao","doi":"10.1080/01478885.2023.2170772","DOIUrl":"https://doi.org/10.1080/01478885.2023.2170772","url":null,"abstract":"In the 1800s, scientists discovered a useful alternative to in vivo research, the in vitro cell culture system. Cell culture has been widely used in academia and industry for diverse issues such as cell biology research to vaccine production and cancer drug discoveries. This article will provide a brief introduction and summary of cell culture history with some key milestones and discuss the future of cell culture from the author’s perspective. When talking about cells, what comes to mind? For me, it is the most well-known cell line, HeLa. HeLa cells were the first immortalized human cell line and may be one of the most important scientific discoveries of the last century. In 1951, HeLa cells were isolated and propagated from cervical cancer tissue from a patient named Henrietta Lacks (HeLa) by Johns Hopkins researcher, Dr George Gey. Since then, this cell line has played an important role in many scientific discoveries, from outer space research to the COVID-19 vaccines, and of course, cancer research all over the world. Other important cell lines include the hybridomas established in 1975 (hybrid cell lines that produce antibodies), mouse embryonic stem cells (ESCs) established in 1981, and human ESCs in 1998. Most recently, in 2006, the world was amazed by the exciting discovery of induced pluripotent stem cells (iPSCs) which were reprogrammed from adult cells back to ESC-like states as a promising alternative to ESCs. In both basic and translational research, cell culture is an indispensable tool. It is becoming more important with the rapid development of reprogramming, gene editing, and in vitro differentiation as well as threedimensional (3D) culture and bioprinting technologies.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 1","pages":"1-4"},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10841996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}