Journal of Histotechnology最新文献_第6页

The placenta: basics and clinical significance 胎盘：基础和临床意义

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-04-03 DOI: 10.1080/01478885.2023.2204610

S. Lau

引用次数: 0

Immunohistochemical characterization of stem cell, vascular, neural, and differentiation markers in the apical papilla and dental pulp of human teeth at various stages of root development. 人牙根发育不同阶段的根尖乳头和牙髓中干细胞、血管、神经和分化标志物的免疫组织化学表征。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-03-01 DOI: 10.1080/01478885.2022.2122665

Cristina Retana-Lobo, Jessie Reyes-Carmona

{"title":"Immunohistochemical characterization of stem cell, vascular, neural, and differentiation markers in the apical papilla and dental pulp of human teeth at various stages of root development.","authors":"Cristina Retana-Lobo, Jessie Reyes-Carmona","doi":"10.1080/01478885.2022.2122665","DOIUrl":"https://doi.org/10.1080/01478885.2022.2122665","url":null,"abstract":"This study aimed to evaluate the expression of several differentiation markers in the apical papilla (AP) and dental pulp (DP) of human permanent teeth. Twenty young human teeth were extracted and classified according to three Moorrees tooth development stages: initial root formation (Ri), root length ½ (R1/2), and root length complete (Rc). Immunohistochemical assays were performed using STRO-1, VEGF Receptor-2, Neurofilament heavy (NFH), and Nestin antibodies and analyzed under light microscopy. Decalcified, formalin fixed paraffin embedded tooth sections stained with hematoxylin and eosin showed an apical cell rich zone between the DP and AP. The AP revealed fewer vascular and cellular components than the DP. STRO-1 was expressed on vascular and neuronal elements beneath the odontoblast (OB) and in the sub-odontoblastic (SOB) zone, and VEGFR-2 positive cells were observed in the endothelium, arterioles, and blood vessels. Neuroepithelial stem cell protein (Nestin) was highly expressed in differentiated odontoblasts in the predentin odontotoblast and odontoblast cell processes. Neurofilament heavy (NFH) was expressed in mature axons throughout the DP. STRO-1 and VEGFR-2 microvascular expression was higher at the stages Ri and R1/2 while STRO-1 and NFH expression showed strong spatial distribution of Rc neuronal elements as compared to Ri and R1/2. Differentiated OB and SOB cells showed Nestin expression, indicating a reservoir of newly differentiated odontoblast-like cells.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10840443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Attenuation of diethylnitrosamine-induced hepatocellular carcinoma in a rat model by combination therapy of diacerein and gold nanoparticles: a histopathological and immunohistochemical study. 二乙基亚硝胺和金纳米颗粒联合治疗对大鼠模型中二乙基亚硝胺诱导的肝细胞癌的抑制作用:组织病理学和免疫组织化学研究。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-03-01 DOI: 10.1080/01478885.2022.2129935

Tourki A S Baokbah

{"title":"Attenuation of diethylnitrosamine-induced hepatocellular carcinoma in a rat model by combination therapy of diacerein and gold nanoparticles: a histopathological and immunohistochemical study.","authors":"Tourki A S Baokbah","doi":"10.1080/01478885.2022.2129935","DOIUrl":"https://doi.org/10.1080/01478885.2022.2129935","url":null,"abstract":"The purpose of this study was to investigate the effect of combined therapy of diacerein and gold nanoparticles (AuNP) on diethylnitrosamine (DEN) induced hepatocellular carcinoma (HCC) in a rat model. Normal healthy and DEN-induced (HCC) rats were divided into five groups. Group I healthy rats served as normal control, Group II untreated HCC rats, Group III HCC rats administered diacerein, Group IV HCC rats administered AuNP, and Group V HCC rats administered diacerein and AuNP. All treatments were given once daily for 4 weeks. Liver morphology and necroinflammation in all groups were evaluated using hematoxylin and eosin (H&E), Masson's trichrome for fibrosis, and immunohistochemistry assays for expression of TNF-α, IL-6, β-catenin, and caspase-3. Liver sections from Group II HCC rats showed loss of lobular architecture, thick fibrous tissue deposition, leukocyte infiltration, degenerated hepatocytes and HCC neoplastic nodules surrounded by extensive fibrosis. Group II had high expression of TNF-α, IL-6, and β-catenin, and low caspase-3 expression as compared to Group I. HCC rats treated with the combined therapy of diacerein and AuNP (Group V) showed markedly decreased HCC lesions, significant necroinflammation reduction (p ˂ 0.05) and 90% reduction in fibrosis as compared to Group II HCC + diacerein. This combined therapy also reduced (p ˂ 0.05) TNF-α, IL-6, β-catenin expression and increased caspase-3 expression. In conclusion, diacerein combined with AuNP synergistically attenuated the severity of HCC lesions by reducing necroinflammation and fibrosis, decreased TNF-α, IL-6, β-catenin expression, and increased caspase-3 expression for apoptosis.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9095767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological changes in the digestive tract of the Chinese soft-shelled turtle (Pelodiscus sinensis) during embryonic development. 中国软甲鱼胚胎发育过程中消化道形态的变化。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-03-01 DOI: 10.1080/01478885.2022.2105490

Haili Zhang, Hongsong Wu, Wen Lu, Yanli Chang, Chunhua Li, Dechang Chu, Yan Chen, Xue Han, Na Li

引用次数: 0

Ulinastatin inhibits microglia activation in spinal cord via P2Y12 receptor in a rat neuropathic pain model. 在大鼠神经性疼痛模型中，乌司他丁通过P2Y12受体抑制脊髓小胶质细胞激活。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-03-01 DOI: 10.1080/01478885.2022.2163792

Ying Shi, Huizhong Wen, Jian Cui, Wanxiang Qin

{"title":"Ulinastatin inhibits microglia activation in spinal cord via P2Y12 receptor in a rat neuropathic pain model.","authors":"Ying Shi, Huizhong Wen, Jian Cui, Wanxiang Qin","doi":"10.1080/01478885.2022.2163792","DOIUrl":"https://doi.org/10.1080/01478885.2022.2163792","url":null,"abstract":"Ulinastatin, a broad spectrum of serine protease inhibitor, has been found to alleviate neuropathic pain (NPP). However, its mechanism is not completely clear. Here, a sciatic nerve ligation rat model and BV2 microglial cells were used to investigate the effect of Ulinastatin on the activation of microglia and P2Y12 receptors in vivo and in vitro. Levels of P2Y12 receptor and NF-κB (P65) expression in the dorsal horn of the lumbar enlargement region of the spinal cord and BV2 cells were assessed by immunohistochemistry and double-label immunofluorescence assays. Levels of IL-1β and TNF-α in cell culture medium and cerebrospinal fluid (CSF) were examined by ELISA. The results showed that Ulinastatin reduced the release of inflammatory IL-1β and TNF-α by inhibiting the activation of spinal microglia. Ulinastatin down-regulated P2Y12 receptor and NF-κB (P65) expression in the spinal microglia of the chronic constrictive injury model. The results indicated that Ulinastatin may attenuate the activation of spinal microglia after peripheral nerve injury by inhibiting the activation of P2Y12 receptor signal pathway in microglia. NF-kB may play a key role in the mechanism of Ulinastatin.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9096747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cell culture: in vitro model system and a promising path to in vivo applications. 细胞培养:体外模型系统及其在体内应用的前景。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-03-01 DOI: 10.1080/01478885.2023.2170772

Chongbei Zhao

{"title":"Cell culture: in vitro model system and a promising path to in vivo applications.","authors":"Chongbei Zhao","doi":"10.1080/01478885.2023.2170772","DOIUrl":"https://doi.org/10.1080/01478885.2023.2170772","url":null,"abstract":"In the 1800s, scientists discovered a useful alternative to in vivo research, the in vitro cell culture system. Cell culture has been widely used in academia and industry for diverse issues such as cell biology research to vaccine production and cancer drug discoveries. This article will provide a brief introduction and summary of cell culture history with some key milestones and discuss the future of cell culture from the author’s perspective. When talking about cells, what comes to mind? For me, it is the most well-known cell line, HeLa. HeLa cells were the first immortalized human cell line and may be one of the most important scientific discoveries of the last century. In 1951, HeLa cells were isolated and propagated from cervical cancer tissue from a patient named Henrietta Lacks (HeLa) by Johns Hopkins researcher, Dr George Gey. Since then, this cell line has played an important role in many scientific discoveries, from outer space research to the COVID-19 vaccines, and of course, cancer research all over the world. Other important cell lines include the hybridomas established in 1975 (hybrid cell lines that produce antibodies), mouse embryonic stem cells (ESCs) established in 1981, and human ESCs in 1998. Most recently, in 2006, the world was amazed by the exciting discovery of induced pluripotent stem cells (iPSCs) which were reprogrammed from adult cells back to ESC-like states as a promising alternative to ESCs. In both basic and translational research, cell culture is an indispensable tool. It is becoming more important with the rapid development of reprogramming, gene editing, and in vitro differentiation as well as threedimensional (3D) culture and bioprinting technologies.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10841996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Practical gynecologic pathology: frequently asked questions 妇科实用病理学：常见问题

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2023-01-02 DOI: 10.1007/978-3-030-68608-6

S. Lau

引用次数: 0

Practical gynecologic pathology: frequently asked questions 实用妇科病理学:常见问题

4区生物学

Journal of Histotechnology Pub Date : 2023-01-02 DOI: 10.1080/01478885.2023.2165726

Stephen K. Lau

引用次数: 0

Test Your Knowledge. 测试你的知识。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-12-01 Epub Date: 2022-10-12 DOI: 10.1080/01478885.2022.2126110

Brooke H Dubansky, Glenda F Hood

引用次数: 0

A study on the safety and therapeutic effect of Xiaoyaosan on depressive disorder related dry eye disease in a murine animal model. 消药散对抑郁症相关性干眼病小鼠模型的安全性及疗效研究。

IF 1.1 4区生物学

Journal of Histotechnology Pub Date : 2022-12-01 Epub Date: 2022-10-31 DOI: 10.1080/01478885.2022.2136818

Jie Li, Ke Yan, Zhaolin Liu, Xiang Lin, Yu Huang, Jian Shi, Dongdong Li, Xiaolei Yao, Zuguo Liu, Qinghua Peng

{"title":"A study on the safety and therapeutic effect of Xiaoyaosan on depressive disorder related dry eye disease in a murine animal model.","authors":"Jie Li, Ke Yan, Zhaolin Liu, Xiang Lin, Yu Huang, Jian Shi, Dongdong Li, Xiaolei Yao, Zuguo Liu, Qinghua Peng","doi":"10.1080/01478885.2022.2136818","DOIUrl":"https://doi.org/10.1080/01478885.2022.2136818","url":null,"abstract":"This study was done to observe the safety and effect of Xiaoyaosan (XYS) on the stimulated depressive disorder (DD) related dry eye disease (DED) in mice. Normal control (NC) group, Vehicle group, and drug treatment groups, including Sertraline Hydrochloride (SH), XYS low-dose (XYS-LD), medium-dose (XYS-MD), and high-dose (XYS-HD), were established. The drug quality of XYS was assessed by high-performance liquid chromatography (HPLC). XYS toxicity in kidney and liver was assessed with Hematoxylin & Eosin (H&E) staining. Serum enzyme-linked immunosorbent assay (ELISA) and body weights were used to evaluate the depression status of mice. Tear production, corneal sensitivity, Oregon Green Dye (OGD) staining, and corneal confocal microscopy were used to assess ocular surface changes. H&E staining was also used to assess pathological cornea and lacrimal gland changes. HPLC results showed that XYS complied with Chinese drug quality standards. The drug treatment groups observed no drug toxicity reactions in the liver and kidney. SH and XYS groups improved DD-related serological indices as compared with Vehicle. Body weight was enhanced in mice with XYS groups compared to Vehicle and SH. Mice with XYS treatments showed increased tear production and corneal sensitivity, decreased corneal OGD staining scores, improved morphology, and decreased inflammatory cell infiltration in the cornea and lacrimal gland. In conclusion, XYS had no drug toxicity, improved serological indices, and ocular surface pathological changes in DD-related DED mice. XYS is safe and may have a therapeutic effect on DD-related DED.","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40436560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1