PhenoCycler®Fusion流式细胞玻片的苏木精和伊红染色。

IF 0.6 4区生物学 Q4 CELL BIOLOGY

Journal of Histotechnology Pub Date : 2023-12-01 Epub Date: 2023-08-16 DOI:10.1080/01478885.2023.2245182

Tomoe Shiomi, Anna Eichinger, Luis Chiriboga

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引用次数: 0

摘要

多路成像技术是一种强大的技术，可以在单细胞空间分辨率下实现整个组织切片的超高plex空间表型。通过索引(CODEX)多路复用共同检测可以检测多达100种蛋白质，使用DNA偶联抗体循环检测应用于组织切片。然而，有必要将多路荧光(mIF)空间图像与分析后的苏木精和伊红(H&E)染色切片相关联。为了有效地将mIF空间图像与H&E形态相关联，开发了一种(H&E)染色方案，该方案在分析后直接应用于CODEX Fusion流式细胞载玻片，允许直接与mIF图像进行H&E关联和注释。

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Hematoxylin and Eosin staining of PhenoCycler® Fusion flow cell slides.

Multiplexed Imaging technologies are powerful techniques that enable ultrahigh-plex spatial phenotyping of whole tissue sections at single cell spatial resolution. Co-Detection by Indexing (CODEX) multiplexing can detect up to 100 proteins using cyclic detection of DNA conjugated antibodies applied to tissue sections. However, it is necessary to correlate multiplexed fluorescent (mIF) spatial images with Hematoxylin and Eosin (H&E) stained sections post analysis. To effectively correlate mIF spatial images with H&E morphology, an (H&E) staining protocol was developed that is directly applied to the CODEX Fusion flow-cell slide after analysis allowing for direct H&E correlation and annotation with mIF images.

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来源期刊

Journal of Histotechnology 生物-细胞生物学

CiteScore

2.60

自引率

9.10%

发文量

审稿时长

>12 weeks

期刊介绍： The official journal of the National Society for Histotechnology, Journal of Histotechnology, aims to advance the understanding of complex biological systems and improve patient care by applying histotechniques to diagnose, prevent and treat diseases. Journal of Histotechnology is concerned with educating practitioners and researchers from diverse disciplines about the methods used to prepare tissues and cell types, from all species, for microscopic examination. This is especially relevant to Histotechnicians. Journal of Histotechnology welcomes research addressing new, improved, or traditional techniques for tissue and cell preparation. This includes review articles, original articles, technical notes, case studies, advances in technology, and letters to editors. Topics may include, but are not limited to, discussion of clinical, veterinary, and research histopathology.