Journal of Histotechnology最新文献

{"title":"Perturbation of mitochondrial dynamics links to the aggravation of periodontitis by diabetes.","authors":"Xinliang Fu,&nbsp;Beilei Liu,&nbsp;Jiyu Sun,&nbsp;Xidan Zhang,&nbsp;Zhuoli Zhu,&nbsp;Hao Wang,&nbsp;Anqi Xiao,&nbsp;Xueqi Gan","doi":"10.1080/01478885.2023.2188705","DOIUrl":"10.1080/01478885.2023.2188705","url":null,"abstract":"<p><p>Diabetes and periodontitis are prevalent diseases that considerably impact global economy and diabetes is a major risk factor of periodontitis. Mitochondrial dynamic alterations are involved in many diseases including diabetes and this study aims to evaluate their relevance with diabetes aggravated periodontitis. Sixty mice are randomly divided into 4 groups: control, periodontitis, diabetes and diabetic periodontitis. Periodontitis severity is evaluated by alveolar bone loss, inflammation and oxidative stress status. Mitochondrial structural and functional defects are evaluated by the mitochondrial fission/fusion events, mitochondrial reactive oxygen species (ROS) accumulation, complex activities and adenosine triphosphate (ATP) production. Advanced glycation end product (AGE) and <i>Porphyromonas gingivalis</i> are closely related to periodontitis occurrence and development. Human gingival fibroblast cells (HGF-1) are used to investigate the AGE role and lipopolysaccharide (LPS) from <i>Porphyromonas gingivalis</i> (P-LPS) in aggravating diabetic periodontitis by mitochondrial dynamic and function alterations. In vivo, diabetic mice with periodontitis show severe bone loss, increased inflammation and oxidative stress accumulation. Among mice with periodontitis, diabetic mice show worse mitochondrial dynamic perturbations than lean mice, along with fusion protein levels inducing more mitochondrial fission in gingival tissue. In vitro, AGEs and P-LPS co-treatment causes severe.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10079203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histotechnology education perspective.","authors":"Sheila Criswell","doi":"10.1080/01478885.2023.2236389","DOIUrl":"https://doi.org/10.1080/01478885.2023.2236389","url":null,"abstract":"Currently in the U.S.A., there are 48 advertised programs to be found through online searches which instruct students in the practice of histotechnology. However, student accessibility to these programs is limited as the programs are found in only 24 (48%) of all states. Texas leads the nation in histotechnology education with 7 programs while Florida hosts 5 programs and Pennsylvania offers 4 programs. Several other states have 2-3 programs and there are 2 programs which are offered exclusively online for the theory portion and remotely for the laboratory experience. Of the 48 programs offered, 35 are associate level histotechnician (HT) only, 8 programs are bachelor level histotechnologist (HTL) only, and 5 more programs appear to offer both HT and HTL certifications. Four of the HTL programs are offered at the master’s level. For many employers, there are no differences between the duties and compensation of HTand HTL-certified personnel. However, in most instances, there are a few advantages in HTL over HT certification which may include increased pay, increased responsibility, more rapid and higher advancement, and possibly a modicum of prestige. There are a few institutions which only hire HTLcertified employees and some locations which only allow HTL-certified personnel to work in immunohistochemistry and special stains areas of the laboratory. Once certified, it is important for the graduate to realize that certification must be maintained. Every three years, an HTor HTLcertified person must obtain a minimum of 36 continuing education credits and submit the renewal application with appropriate fees in order to maintain the certification. Histotechnology educational programs are accredited by the National Accrediting Agency for Clinical Laboratory Sciences (NAACLS), the same body which oversees medical laboratory science programs. NAACLS accredited histotechnology programs are uniformly composed of a didactic theory portion and a hands-on clinical portion. However, exactly how those times are divided and the method of delivery, in large part, are left up to the program administrators. Didactic portions may be delivered in person, online, or a hybrid version of the two. Laboratory experience may be in a tuitionsupported student laboratory, in a tuition-supported clinical laboratory, or in a paid position as a trainee in a clinical laboratory. In order to qualify for registration for the American Society of Clinical Pathology (ASCP) board of certification exam, an applicant must demonstrate proficiency in tissue fixation, processing, embedding, microtomy, staining, and laboratory operations, all of which NAACLS ensures is part of the curriculum when accrediting a program. Particularly in regions of the country without programs, and often even in areas with programs, histotechnology can be taught on the job, and, after a year of full-time experience, a person with adequate college coursework may apply to take the ASCP HT or HTL examination. P","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10480336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of pathological processes of meibomian gland dysfunction by in vitro culture airlifting conditions.","authors":"Wenjia Zhang,&nbsp;Shuxian Hu,&nbsp;Hongqin Ke,&nbsp;Zhengyilin Bao,&nbsp;Hai Liu,&nbsp;Zhulin Hu","doi":"10.1080/01478885.2023.2199370","DOIUrl":"10.1080/01478885.2023.2199370","url":null,"abstract":"<p><p>Meibomian gland dysfunction (MGD) is a group of disorders linked by functional abnormalities of the meibomian glands. Current studies on MGD pathogenesis focus on meibomian gland cells, providing information on a single cell's response to experimental manipulation, and do not maintain the architecture of an intact meibomian gland acinus and the acinar epithelial cells' secretion state in vivo. In this study, rat meibomian gland explants were cultured by a Transwell chamber-assisted method under an air-liquid interface (airlift) in vitro for 96 h. Analyses for tissue viability, histology, biomarker expression, and lipid accumulation were performed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and TUNEL assays, hematoxylin and eosin (H&E) staining, immunofluorescence, Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), transmission electron microscopy (TEM), and western blotting (WB). MTT, TUNEL, and H&E staining indicated better tissue viability and morphology than the submerged conditions used in previous studies. Levels of MGD biomarkers, including keratin 1 (KRT1) and 14 (KRT14) and peroxisome proliferator-activated receptor-gamma (PPAR-γ), along with oxidative stress markers, including reactive oxygen species, malondialdehyde, and 4-hydroxy-2-nonenal, gradually increased over culture time. The MGD pathophysiological changes and biomarker expression of meibomian gland explants cultured under airlift conditions were similar to those reported by previous studies, indicating that abnormal acinar cell differentiation and glandular epithelial cell hyperkeratosis may contribute to obstructive MGD occurrence.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10068584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atlas of sinonasal tract pathology","authors":"Stephen K. Lau","doi":"10.1080/01478885.2023.2236387","DOIUrl":"https://doi.org/10.1080/01478885.2023.2236387","url":null,"abstract":"Published in Journal of Histotechnology (Vol. 46, No. 3, 2023)","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138504227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atlas of sinonasal tract pathology","authors":"S. Lau","doi":"10.1007/978-981-19-7315-4","DOIUrl":"https://doi.org/10.1007/978-981-19-7315-4","url":null,"abstract":"","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45898504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Verhoeff van Gieson staining in laser-irradiated gingival tissues.","authors":"Joseph A Esposito,&nbsp;Nathan Estrin,&nbsp;Steve A McClain,&nbsp;Wei Hou,&nbsp;Georgios E Romanos","doi":"10.1080/01478885.2023.2193527","DOIUrl":"https://doi.org/10.1080/01478885.2023.2193527","url":null,"abstract":"<p><p>There have been several studies on the use of the Verhoeff van Gieson staining method to demonstrate thermal effects on tissues. However, this method has rarely been used for the analysis of periodontal tissues. This study was undertaken to compare the quality and effectiveness of the Verhoeff van Gieson (VVG) staining method with conventional hematoxylin & eosin (H&E) in measuring the thermal effects in gingival tissues. Periodontal tissues around bovine mandibular teeth were treated using different surgical lasers (wavelengths of 10,600 nm, 970 nm, and 445 nm) at 2 W power setting. Measurements of the depth of the coagulation zone were recorded for all treatment groups in sample tissues stained with H&E as well as the VVG-staining method. Measures were interpreted by a trained pathologist. A statistical analysis was performed using the Wilcoxon signed-rank test to determine if there was a statistically significant difference between values recorded for the light penetration depth on tissues stained with each of the two staining methods. It was determined that there was no significant difference in the recorded values (<i>P</i> = 0.23). We have concluded that the VVG-stained tissues were better able to visualize the depth of thermal damage and thus may make it easier for someone not well trained to interpret the depth of light penetration in these tissues.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9660068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The protective effect of erythropoietin on ischemia- reperfusion injury caused by ovarian torsion-detorsion in the experimental rat model.","authors":"Bahar Kartal,&nbsp;Mehmet Fatih Bozkurt,&nbsp;Ebru Alimoğullari,&nbsp;Uygar Saçık","doi":"10.1080/01478885.2022.2122653","DOIUrl":"https://doi.org/10.1080/01478885.2022.2122653","url":null,"abstract":"<p><p>Ovarian torsion is one of the most dangerous gynecological emergencies requiring surgery. A total of 50%-90% ovarian torsion cases are caused by physiological cysts, endometriosis, and other benign or malignant ovarian neoplasms. The aim of the study was to investigate the effects of erythropoietin (EPO) treatment on ischemia/reperfusion (IR) injury caused by ovarian torsion/detorsion (T/D) injury. Thirty female Wistar albino rats were divided into five groups as follows: Group I: Control; Group II: Torsion (T); Group III: Torsion/Detorsion(T/D); Group IV: Torsion/Detorsion (T/D) + EPO; Group V: EPO. Sections of the ovaries were evaluated for histopathological changes with hematoxylin and eosin stain, a immunohistochemical assay for caspase 3 expression, and the TUNEL assay for apoptosis. Ovarian sections from torsion/detorsion and torsion groups showed more hemorrhage, vascular congestion, edema, degenerative granulosa, and stromal cells. Fewer histopathological changes were found in EPO and T/D + EPO groups. Caspase 3 and TUNEL positive cells were significantly increased in the torsion/detorsion group as compared with the other groups (<i>p</i> < 0.05). Treatment with erythropoietin decreased the number of caspase 3 and TUNEL positive cells. The results of the study showed that erythropoietin administration is effective for recovery from degenerative changes in the ovary induced by the torsion-detorsion injury.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9712663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epithelial ovarian carcinoma - a perspective.","authors":"Pranav S Renavikar,&nbsp;Jie Chen,&nbsp;Subodh M Lele","doi":"10.1080/01478885.2023.2204609","DOIUrl":"https://doi.org/10.1080/01478885.2023.2204609","url":null,"abstract":"The subtypes of epithelial ovarian carcinoma have differing pathophysiology, behavior, molecular alterations, and treatment approaches. As one of the most common malignancies diagnosed in females, ovarian carcinoma has the highest case-to-fatality ratio, primarily due to serous carcinoma presenting at an advanced stage with wide peritoneal metastasis and occasional lymph node metastasis. In comparison, the non-serous carcinomas, which most commonly include endometrioid and clear cell carcinomas, are frequently diagnosed at an early stage. Within serous carcinoma, low-grade and high-grade serous carcinoma are two distinct tumor types. Lowgrade serous carcinoma is thought to arise from precursor lesions like serous borderline tumor. In contrast, high-grade serous carcinoma is not related to a precursor lesion and thought to arise directly from malignant transformation of the ovarian surface epithelium (incessant ovulation theory) or from the epithelium of cortical inclusion cysts (resulting from exfoliated tubal epithelium implanting on the ovarian surface during ovulation). Recently, studies have shown that many high-grade serous carcinomas arise from the tubal fimbria from serous tubal intraepithelial carcinoma, particularly in BRCA-positive patients. BRAF, KRAS, and ERBB2 mutations are important molecular events in low-grade serous carcinoma while high-grade serous carcinoma is almost always associated with a TP53 mutation. Primary ovarian mucinous carcinoma is uncommon and needs workup to exclude metastasis from a gastrointestinal source. Like low-grade serous carcinoma, mucinous carcinoma is shown to arise from the adenoma-carcinoma sequence involving mucinous cystadenoma and mucinous borderline tumor, often coexisting in the same neoplasm. A high percentage of mucinous ovarian carcinomas have KRAS mutations. Most ovarian endometrioid adenocarcinomas are usually low-grade, unilateral and early-stage. They are often associated with endometriosis, endometriotic cyst, or endometrioid borderline tumor. Concurrently, the uterus may demonstrate atypical hyperplasia or endometrioid adenocarcinoma, as molecular events including PTEN, CTNNB1, KRAS and PIK3A mutations are shared between endometrioid adenocarcinoma of the two organs. Primary ovarian clear cell carcinoma occurs with a similar frequency, and shares some features with endometrioid adenocarcinoma, including early stage presentation and association with endometriosis. Recent studies have identified ARID1A mutations in most of these tumors [1].","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10015803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of mTOR signal pathway in MMTV-TGFα mice ovary at different ages.","authors":"T Onel,&nbsp;E Yıldırım,&nbsp;S Dogan,&nbsp;A Yaba","doi":"10.1080/01478885.2022.2109883","DOIUrl":"https://doi.org/10.1080/01478885.2022.2109883","url":null,"abstract":"<p><p>Transforming growth factor alpha (TGFα), a member of the epidermal growth factor (EGF) family, regulates cell proliferation, differentiation, and development, and involves follicular development and viability. In ovaries, TGFα is shown localized in granulosa cells (GCs) of primary follicles, theca cells (TCs) of pre-antral, antral and pre-ovulatory follicles. TGFα overexpression in mouse mammary tumor virus (MMTV-TGFα) transgenic mice causes mammary tumor after 50 weeks. However, follicular development and preservation of the ovarian follicle reserve-mediating follicle stimulating hormone (FSH) response are unknown. Mammalian target of rapamycin (mTOR) is a key regulator for cell proliferation, growth, differentiation, and apoptosis, and important for ovarian folliculogenesis and oocyte maturation. The study aim determines TGFα overexpression during folliculogenesis via mTOR signaling pathway in ovaries from 10-, 18-, 50-, and 82-week-old MMTV-TGFα mice. Histological analysis was performed, along with western blot for mTOR, p-mTOR, P70S6K, PCNA, and Caspase-3, and quantitative RNA (qRT-PCR) for <i>mTOR</i> and <i>P70S6K</i>. Developing follicles number decreased and atretic follicles number increased with aging in MMTV-TGFα mice ovary. Ovaries at 18 and 82 weeks had decreased PCNA and increased Caspase-3 protein expression levels as compared to 10-week ovaries. Protein expression levels of mTOR and p-mTOR decreased gradually from ovaries at 10-18 weeks, increased at 50 weeks and decreased again at 82 weeks. These results indicate that TGFα may be one regulator of healthy follicular development and affect <i>mTOR</i> signaling pathway during ovarian aging. Thus, over-expression of TGFα might lead to reduced ovarian reserve and premature ovarian insufficiency.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10014202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulatory effects of concomitant quercetin/sitagliptin administration on the ovarian histological and biochemical alterations provoked by doxorubicin in a streptozotocin-induced diabetic rat model.","authors":"Ahmed A Morsi,&nbsp;Eman Mohamed Faruk,&nbsp;Engy Medhat,&nbsp;Neama M Taha,&nbsp;Usama Fouad Ahmed Ebrahim","doi":"10.1080/01478885.2022.2105481","DOIUrl":"https://doi.org/10.1080/01478885.2022.2105481","url":null,"abstract":"<p><p>Limited literature was available on the effects of sitagliptin or quercetin treatments on doxorubicin induced ovarian dysfunction in diabetic animals. The study aim was test the efficacy and suggested mechanisms of quercetin/sitagliptin combined treatment on the doxorubicin-induced ovarian toxicity in rat model with streptozotocin-induced diabetes. Forty eight female Wistar rats were divided into six groups: 1) Control; 2) Streptozotocin induced diabetes; 3) Streptozotocin-induced diabetes + doxorubicin ovarian damage; 4) Streptozotocin-induced diabetes + doxorubicin ovarian damage with; 5) Streptozotocin-induced diabetes + doxorubicin ovarian damage with sitagliptin treatment and 6) Streptozotocin-induced diabetes + doxorubicin ovarian damage with concomitant quercetin/sitagliptin treatment. Biochemical tests for serum estrogen, progesterone, insulin, blood glucose, and ovarian levels of malondialdehyde, nitric oxide, and superoxide dismutase and qRT-PCR for NOBOX, FSHr, and iNOS genes were performed. Histological evaluation was done on ovary sections with hematoxylin and eosin and immunohistochemistry for 8-OHdG and iNOS followed by morphometric analysis. The streptozotocin-induced diabetic group showed varying degrees of follicle atresia and altered biochemical parameters, both were marked in the streptozotocin-induced diabetic + doxorubicin group. The mRNA of NOBOX, FSHr, and iNOS genes were disturbed with increased immunoexpression of iNOS and 8-OHdG. Quercetin and/or sitagliptin administration improved all altered histological and biochemical parameters and was more effective as a combined treatment. The study suggested equal efficacy of both quercetin and sitagliptin in mitigating the doxorubicin-induced ovarian toxicity in the streptozotocin diabetic rat model, and the combined therapy showed anti-inflammatory, anti-antioxidant, and anti-DNA damage mechanisms.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":null,"pages":null},"PeriodicalIF":1.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9656197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}