Journal of Histotechnology最新文献

{"title":"Hematoxylin and Eosin staining of PhenoCycler® Fusion flow cell slides.","authors":"Tomoe Shiomi, Anna Eichinger, Luis Chiriboga","doi":"10.1080/01478885.2023.2245182","DOIUrl":"10.1080/01478885.2023.2245182","url":null,"abstract":"<p><p>Multiplexed Imaging technologies are powerful techniques that enable ultrahigh-plex spatial phenotyping of whole tissue sections at single cell spatial resolution. Co-Detection by Indexing (CODEX) multiplexing can detect up to 100 proteins using cyclic detection of DNA conjugated antibodies applied to tissue sections. However, it is necessary to correlate multiplexed fluorescent (mIF) spatial images with Hematoxylin and Eosin (H&E) stained sections post analysis. To effectively correlate mIF spatial images with H&E morphology, an (H&E) staining protocol was developed that is directly applied to the CODEX Fusion flow-cell slide after analysis allowing for direct H&E correlation and annotation with mIF images.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"203-206"},"PeriodicalIF":1.1,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10061598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of iron and ferroptosis in the pathogenesis of acute pancreatitis.","authors":"Jin Tao, Yiyi Zhang, Yinshi Huang, Min Xu","doi":"10.1080/01478885.2023.2261093","DOIUrl":"10.1080/01478885.2023.2261093","url":null,"abstract":"<p><p>Acute pancreatitis (AP) is an inflammatory disease of the pancreas. Iron is an essential element for life and is involved in many metabolic processes. Ferroptosis is a type of regulated cell death that is triggered by iron and oxidative stress. A well-established mouse AP model was adopted to study the role of iron and ferroptosis in the pathogenesis of pancreatitis. Mice were injected with cerulein to induce AP, and pancreatic tissue samples were analyzed to determine the pathology, cell death, iron deposition, expression of iron transporters, and lipid peroxidation. The role of iron was studied by giving mice extra iron or iron chelator. In vitro studies with acinar cells with ferroptosis activator and inhibitor were also performed to assess the inflammatory response. Iron was found accumulated in the pancreatic tissue of mice who suffered cerulein-induced pancreatitis. Cell death and lipid peroxidation increased in these tissues and could be further modulated by iron dextran or iron chelator. Mice given Hemin through gavage had reduced levels of GSH in pancreatic tissue and increased inflammatory response. Studies with acinar cells showed increased levels of lipid peroxidation and ferroptosis-specific mitochondrial damage when treated with ferroptosis inducer and inflammatory cytokines.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"184-193"},"PeriodicalIF":1.1,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41203066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The burden of uterine fibroids: an overview.","authors":"Jitu W George","doi":"10.1080/01478885.2023.2265185","DOIUrl":"10.1080/01478885.2023.2265185","url":null,"abstract":"","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":" ","pages":"153-155"},"PeriodicalIF":0.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41122257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The thorax: medical, radiological and pathological assessment <b>The thorax: medical, radiological and pathological assessment</b> , edited by Moran, Cesar A., Truong, Mylene T., de Groot, Patricia M., Cham (Switzerland), Springer, 2023, 934 (+xvi) pp., $249.99 (hardcover), $189.00 (e-book), ISBN: 978-3-031-21039-6, ISBN: 978-3-031-21040-2 (e-book)","authors":"Stephen K. Lau","doi":"10.1080/01478885.2023.2268935","DOIUrl":"https://doi.org/10.1080/01478885.2023.2268935","url":null,"abstract":"\"The thorax: medical, radiological and pathological assessment.\" Journal of Histotechnology, ahead-of-print(ahead-of-print), p. 1","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"131 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135858639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological significance of SOX9 and β-catenin expression in pre-neoadjuvant chemotherapy cases of osteosarcoma: molecular and immunohistochemical study.","authors":"Sarah Ahmed Hassan,&nbsp;Amal Abdel Aziz Shabaan,&nbsp;Adel Refaat Ahmed,&nbsp;Yasmine Amr Issa,&nbsp;Shady Hassan Fadel,&nbsp;Bassma Mohamed El-Sabaa","doi":"10.1080/01478885.2023.2193526","DOIUrl":"10.1080/01478885.2023.2193526","url":null,"abstract":"<p><p>The molecular pathogenesis of osteosarcoma (OS), the most frequent primary malignant bone tumor of all age groups, is still obscure. Since multidrug chemotherapeutic regimens were introduced in the 1970s, survival rates have been stationary. The Wnt-β-catenin signaling cascade and SOX9 have a significant contribution to skeletal growth, development, and tumorigenesis. In the present work, an attempt was made to examine the role and clinicopathological significance of β-catenin and SOX9 in 46 cases of pre-neoadjuvant chemotherapy OS tissues compared to 10 cases of non-neoplastic bone. The mRNA levels of both markers were assessed by qRT-PCR, and protein levels of β-catenin were analyzed by immunohistochemistry. The results were correlated with different clinicopathological parameters. SOX9 mRNA levels were significantly elevated in OS compared to non-neoplastic bone, and higher levels were significantly associated with the occurrence of fluid-fluid levels (indicating blood-containing cystic spaces) and osteolytic radiological pattern. Although β-catenin mRNA and protein levels were higher in OS compared to non-neoplastic bone, only the protein levels reached statistical significance. Higher β-catenin mRNA levels were significantly associated with tumor size, while higher protein levels were significantly associated with the histologic subtype, mitotic count, and radiological pattern. No significant association was noted with any of the other evaluated parameters. OS showing higher SOX9 mRNA expression and lower β-catenin mRNA and protein expression exhibited longer estimated overall survival times approaching statistical significance. To conclude, while high expression of β-catenin and SOX9 suggests their possible involvement in OS development, their prognostic role may need further research.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 3","pages":"127-138"},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10203904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histologic examination of canned cat food.","authors":"Levi Haven,&nbsp;Amanda Bodkin,&nbsp;Sheila L Criswell","doi":"10.1080/01478885.2023.2177815","DOIUrl":"10.1080/01478885.2023.2177815","url":null,"abstract":"<p><p>Cat food production is a billion-dollar industry in the United States, with most pet owners trusting pet food companies to provide their pets with complete nutrition. Moist or canned cat food is healthier than dry kibble for cats due to its higher water content promoting healthy kidney function, but ingredient labels on canned cat food are lengthy with ambiguous terminology including 'animal by-products.' Forty canned cat food samples were collected from grocery stores and were processed using routine histologic methods. Hematoxylin and eosin-stained tissue sections were evaluated microscopically to determine the cat food content. Many brands and flavors were composed of well-preserved skeletal muscles admixed with various animal organs, which closely approximates nutritional components found in natural feline prey. However, several samples demonstrated marked degenerative changes suggesting a delay in food processing and potential decrease in nutrient content. Four samples contained cuts consisting of skeletal muscle only with no organ meat. Surprisingly, 10 samples contained fungal spores and 15 demonstrated refractile particulate matter. A cost analysis demonstrated that although the overall quality of canned cat food increases as the average cost per ounce increases, low-cost high-quality canned cat food is available.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 3","pages":"114-126"},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10431707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perturbation of mitochondrial dynamics links to the aggravation of periodontitis by diabetes.","authors":"Xinliang Fu,&nbsp;Beilei Liu,&nbsp;Jiyu Sun,&nbsp;Xidan Zhang,&nbsp;Zhuoli Zhu,&nbsp;Hao Wang,&nbsp;Anqi Xiao,&nbsp;Xueqi Gan","doi":"10.1080/01478885.2023.2188705","DOIUrl":"10.1080/01478885.2023.2188705","url":null,"abstract":"<p><p>Diabetes and periodontitis are prevalent diseases that considerably impact global economy and diabetes is a major risk factor of periodontitis. Mitochondrial dynamic alterations are involved in many diseases including diabetes and this study aims to evaluate their relevance with diabetes aggravated periodontitis. Sixty mice are randomly divided into 4 groups: control, periodontitis, diabetes and diabetic periodontitis. Periodontitis severity is evaluated by alveolar bone loss, inflammation and oxidative stress status. Mitochondrial structural and functional defects are evaluated by the mitochondrial fission/fusion events, mitochondrial reactive oxygen species (ROS) accumulation, complex activities and adenosine triphosphate (ATP) production. Advanced glycation end product (AGE) and <i>Porphyromonas gingivalis</i> are closely related to periodontitis occurrence and development. Human gingival fibroblast cells (HGF-1) are used to investigate the AGE role and lipopolysaccharide (LPS) from <i>Porphyromonas gingivalis</i> (P-LPS) in aggravating diabetic periodontitis by mitochondrial dynamic and function alterations. In vivo, diabetic mice with periodontitis show severe bone loss, increased inflammation and oxidative stress accumulation. Among mice with periodontitis, diabetic mice show worse mitochondrial dynamic perturbations than lean mice, along with fusion protein levels inducing more mitochondrial fission in gingival tissue. In vitro, AGEs and P-LPS co-treatment causes severe.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 3","pages":"139-150"},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10079203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histotechnology education perspective.","authors":"Sheila Criswell","doi":"10.1080/01478885.2023.2236389","DOIUrl":"https://doi.org/10.1080/01478885.2023.2236389","url":null,"abstract":"Currently in the U.S.A., there are 48 advertised programs to be found through online searches which instruct students in the practice of histotechnology. However, student accessibility to these programs is limited as the programs are found in only 24 (48%) of all states. Texas leads the nation in histotechnology education with 7 programs while Florida hosts 5 programs and Pennsylvania offers 4 programs. Several other states have 2-3 programs and there are 2 programs which are offered exclusively online for the theory portion and remotely for the laboratory experience. Of the 48 programs offered, 35 are associate level histotechnician (HT) only, 8 programs are bachelor level histotechnologist (HTL) only, and 5 more programs appear to offer both HT and HTL certifications. Four of the HTL programs are offered at the master’s level. For many employers, there are no differences between the duties and compensation of HTand HTL-certified personnel. However, in most instances, there are a few advantages in HTL over HT certification which may include increased pay, increased responsibility, more rapid and higher advancement, and possibly a modicum of prestige. There are a few institutions which only hire HTLcertified employees and some locations which only allow HTL-certified personnel to work in immunohistochemistry and special stains areas of the laboratory. Once certified, it is important for the graduate to realize that certification must be maintained. Every three years, an HTor HTLcertified person must obtain a minimum of 36 continuing education credits and submit the renewal application with appropriate fees in order to maintain the certification. Histotechnology educational programs are accredited by the National Accrediting Agency for Clinical Laboratory Sciences (NAACLS), the same body which oversees medical laboratory science programs. NAACLS accredited histotechnology programs are uniformly composed of a didactic theory portion and a hands-on clinical portion. However, exactly how those times are divided and the method of delivery, in large part, are left up to the program administrators. Didactic portions may be delivered in person, online, or a hybrid version of the two. Laboratory experience may be in a tuitionsupported student laboratory, in a tuition-supported clinical laboratory, or in a paid position as a trainee in a clinical laboratory. In order to qualify for registration for the American Society of Clinical Pathology (ASCP) board of certification exam, an applicant must demonstrate proficiency in tissue fixation, processing, embedding, microtomy, staining, and laboratory operations, all of which NAACLS ensures is part of the curriculum when accrediting a program. Particularly in regions of the country without programs, and often even in areas with programs, histotechnology can be taught on the job, and, after a year of full-time experience, a person with adequate college coursework may apply to take the ASCP HT or HTL examination. P","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 3","pages":"97-100"},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10480336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of pathological processes of meibomian gland dysfunction by in vitro culture airlifting conditions.","authors":"Wenjia Zhang,&nbsp;Shuxian Hu,&nbsp;Hongqin Ke,&nbsp;Zhengyilin Bao,&nbsp;Hai Liu,&nbsp;Zhulin Hu","doi":"10.1080/01478885.2023.2199370","DOIUrl":"10.1080/01478885.2023.2199370","url":null,"abstract":"<p><p>Meibomian gland dysfunction (MGD) is a group of disorders linked by functional abnormalities of the meibomian glands. Current studies on MGD pathogenesis focus on meibomian gland cells, providing information on a single cell's response to experimental manipulation, and do not maintain the architecture of an intact meibomian gland acinus and the acinar epithelial cells' secretion state in vivo. In this study, rat meibomian gland explants were cultured by a Transwell chamber-assisted method under an air-liquid interface (airlift) in vitro for 96 h. Analyses for tissue viability, histology, biomarker expression, and lipid accumulation were performed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and TUNEL assays, hematoxylin and eosin (H&E) staining, immunofluorescence, Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), transmission electron microscopy (TEM), and western blotting (WB). MTT, TUNEL, and H&E staining indicated better tissue viability and morphology than the submerged conditions used in previous studies. Levels of MGD biomarkers, including keratin 1 (KRT1) and 14 (KRT14) and peroxisome proliferator-activated receptor-gamma (PPAR-γ), along with oxidative stress markers, including reactive oxygen species, malondialdehyde, and 4-hydroxy-2-nonenal, gradually increased over culture time. The MGD pathophysiological changes and biomarker expression of meibomian gland explants cultured under airlift conditions were similar to those reported by previous studies, indicating that abnormal acinar cell differentiation and glandular epithelial cell hyperkeratosis may contribute to obstructive MGD occurrence.</p>","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"46 3","pages":"101-113"},"PeriodicalIF":1.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10068584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atlas of sinonasal tract pathology","authors":"Stephen K. Lau","doi":"10.1080/01478885.2023.2236387","DOIUrl":"https://doi.org/10.1080/01478885.2023.2236387","url":null,"abstract":"Published in Journal of Histotechnology (Vol. 46, No. 3, 2023)","PeriodicalId":15966,"journal":{"name":"Journal of Histotechnology","volume":"55 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138504227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}