一种在低资源环境下采集和保存胎盘标本用于恶性疟原虫鉴定和核酸分析的新方法。

IF 0.6 4区生物学 Q4 CELL BIOLOGY

Journal of Histotechnology Pub Date : 2022-09-01 DOI:10.1080/01478885.2022.2088191

Patrick S Potoczak, Beverly I Strassmann, Claudius Vincenz

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引用次数: 0

摘要

在资源匮乏的环境下，组织标本的收集、保存和运输具有挑战性。我们提出了一种新的方法，通过使用福尔马林固定，乙醇脱水和长期储存在太阳能冰箱中，实现了马里农村胎盘标本的良好保存。样本保存成功率为92%，允许评估当前和过去的疟疾感染、贫血、胎盘成熟度和炎症。使用RNAscope®杂交技术，我们能够在福尔马林固定石蜡包埋(FFPE)标本中可视化细胞特异性基因表达模式。此外，我们的方法需要从子叶的两个切面进行镜像采样，一个用于FFPE工作流程，另一个用于RNAlater™和RNA-seq中存储。

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A new method for the sampling and preservation of placental specimens in low-resource settings for the identification of P. falciparum and analysis of nucleic acids.

Collection, preservation, and shipment of histological specimens in low-resource settings is challenging. We present a novel method that achieved excellent preservation of placental specimens from rural Mali by using formalin fixation, ethanol dehydration, and long-term storage in a solar-powered freezer. Sample preservation success was 92%, permitting evaluation of current and past malaria infection, anemia, placental maturity, and inflammation. Using RNAscope® hybridization we were able to visualize cell-specific gene expression patterns in the formalin-fixed paraffin-embedded (FFPE) specimens. Additionally, our method entailed mirrored sampling from the two cut faces of a cotyledon, one for the FFPE workflows and the other for storage in RNAlater™ and RNA-seq.

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来源期刊

Journal of Histotechnology 生物-细胞生物学

CiteScore

2.60

自引率

9.10%

发文量

审稿时长

>12 weeks

期刊介绍： The official journal of the National Society for Histotechnology, Journal of Histotechnology, aims to advance the understanding of complex biological systems and improve patient care by applying histotechniques to diagnose, prevent and treat diseases. Journal of Histotechnology is concerned with educating practitioners and researchers from diverse disciplines about the methods used to prepare tissues and cell types, from all species, for microscopic examination. This is especially relevant to Histotechnicians. Journal of Histotechnology welcomes research addressing new, improved, or traditional techniques for tissue and cell preparation. This includes review articles, original articles, technical notes, case studies, advances in technology, and letters to editors. Topics may include, but are not limited to, discussion of clinical, veterinary, and research histopathology.