Journal of Helminthology最新文献_第3页

A qPCR assay for sex identification and sex-ratio estimation in Ascaris suum. 猪蛔虫性别鉴定和性别比估计的qPCR方法。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-22 DOI: 10.1017/S0022149X26101722

H Yu, M Lu, H Tian, Y Lu, M Zhou, M Liu, Z Lü, L Han, Z Hou

{"title":"A qPCR assay for sex identification and sex-ratio estimation in Ascaris suum.","authors":"H Yu, M Lu, H Tian, Y Lu, M Zhou, M Liu, Z Lü, L Han, Z Hou","doi":"10.1017/S0022149X26101722","DOIUrl":"https://doi.org/10.1017/S0022149X26101722","url":null,"abstract":"Natural selection is expected to maintain a stable 1:1 sex ratio in randomly mating populations. However, obligate parasites, especially nematodes with complex host-migratory life cycles, often show pronounced departures from this equilibrium. Ascaris suum, a globally distributed nematode with an XX/XO sex-determination system, exhibits an obvious female-biased sex ratio in adults, making it an ideal model to investigate when and how sex-ratio bias arises. Progress has been limited because, at present, no practicable method exists to identify worm sex at stages other than adulthood. Here, we aimed to establish a valid approach designed to be applicable, in principle, for precise sex identification and sex-ratio estimation across all stages of A. suum. Using a chromosome-level A. suum genome assembled in our laboratory (CNP0003380), we identified single-copy sequences on autosomes and the X chromosome and selected 3C (Chr3) as an autosomal marker and 5X (Chr5X) as an X-linked marker. We developed a qPCR assay that infers sex or sex ratio from the 3C/5X copy-number ratio, with theoretical values of 1 for XX females and 2 for XO males. Validation with 30 adult females and 30 adult males yielded 3C/5X ratios of 1.009 ± 0.042 and 1.995 ± 0.054, respectively, with no significant deviation from theoretical expectations (p > 0.05). The relative abundance of X-bearing to O-bearing sperm measured by qPCR was 0.980 ± 0.093, compared with 1.081 ± 0.163 by flow cytometry. Although the mean values differed significantly between the two methods (paired Students t-test, t = -2.886, d.f. = 29, p = 0.0073), the qPCR-based estimates showed lower variability than those obtained by flow cytometry. These results indicate that this qPCR assay provides an accurate tool for sex identification and quantification of the relative abundance of X-bearing to O-bearing sperm in A. suum.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e123"},"PeriodicalIF":2.0,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149017182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

B chromosomes as a hidden driver of intestinal nematode infection in the yellow-necked mouse (Apodemus flavicollis). B染色体作为黄颈鼠肠道线虫感染的隐藏驱动因子。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-21 DOI: 10.1017/S0022149X26101801

B Čabrilo, O Bjelić Čabrilo, B Tošić, I Budinski, M Rajičić, V M Jovanović, J Blagojević

{"title":"B chromosomes as a hidden driver of intestinal nematode infection in the yellow-necked mouse (Apodemus flavicollis).","authors":"B Čabrilo, O Bjelić Čabrilo, B Tošić, I Budinski, M Rajičić, V M Jovanović, J Blagojević","doi":"10.1017/S0022149X26101801","DOIUrl":"https://doi.org/10.1017/S0022149X26101801","url":null,"abstract":"The host-parasite relationship is affected by various biological, ecological, and anthropogenic factors. Some of them, such as host sex, are well studied. On the other hand, the effect of genetic differences, such as the presence of supernumerary or B chromosomes (Bs), has received little attention. We investigated the independent and interactive effects of host sex and B chromosome presence on intestinal nematode abundance in 294 yellow-necked mice (Apodemus flavicollis) from 18 localities in Serbia. Nine nematode species were identified. Because parasite numbers showed high inter-individual variability, we used a Monte Carlo randomisation method to test differences in mean abundance without assuming a specific statistical distribution. Host sex had a limited influence on infection patterns. In line with existing theoretical frameworks, a significant male-biased abundance was detected, but only for Aonchotheca annulosa, whereas no overall sex bias was found for total nematode burden. B chromosome carriers were found to have a significantly higher abundance of Syphacia frederici. A significant interaction between sex and Bs was detected for total intestinal nematodes and Syphacia stroma, with females carrying Bs harbouring the highest parasite loads. The results, indicating that Bs can shape helminth infrapopulation structure and that their effects may be sex-dependent, were discussed. The absence of consistent male-biased parasitism further suggests that sex effects are parasite-specific rather than universal. Our study highlights the importance of considering cryptic genomic variation in host-parasite research and demonstrates the value of Monte Carlo methods for analysing overdispersed helminth abundance data in wildlife hosts.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e122"},"PeriodicalIF":2.0,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recognition of the Antorchiinae Skrjabin & Koval, 1957 as a subfamily of the Zoogonidae for digenean trematodes formerly assigned to the Faustulidae. 承认anorchiinae Skrjabin & Koval， 1957作为虫虫科的一个亚科，以前被分配到Faustulidae。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-21 DOI: 10.1017/S0022149X26101813

S C Cutmore, N Q-X Wee, S B Martin, T H Cribb

{"title":"Recognition of the Antorchiinae Skrjabin & Koval, 1957 as a subfamily of the Zoogonidae for digenean trematodes formerly assigned to the Faustulidae.","authors":"S C Cutmore, N Q-X Wee, S B Martin, T H Cribb","doi":"10.1017/S0022149X26101813","DOIUrl":"https://doi.org/10.1017/S0022149X26101813","url":null,"abstract":"We report new collections of trematodes currently assigned to the Faustulidae Poche, 1926, from fishes of the Pomacanthidae and Ostraciidae in the tropical Indo-West Pacific. Phylogenetic analyses demonstrate that these species, and several other faustulid taxa, form a well-supported clade within the Zoogonidae Odhner, 1902 (Microphalloidea), distant from the Faustulidae sensu stricto (Gymnophalloidea). We recognise the subfamily Antorchiinae Skrjabin & Koval, 1957 within the Zoogonidae for Antorchis Linton, 1911, Neoparantorchis Hafeezullah & Siddiqi, 1971, Parantorchis Yamaguti, 1934, Paradiscogaster Yamaguti, 1934, Discogastroides Strand, 1934, Parayamagutia Machida, 1971 and Trigonocryptus Martin, 1958, and propose four new genera. Scaticola n. g. is proposed for two species of Bacciger Nicoll, 1914 that resolve within the Antorchiinae rather than with Bacciger sensu stricto. Crassitrema n. g. and Findovitellus n. g. are proposed for two species of Paradiscogaster that resolve distant from all other sequenced species of Paradiscogaster. Nasoantorchis n. g. is proposed for Nasoantorchis nasonis (Cribb, Bray, Hall & Cutmore, 2015) n. comb., previously considered a member of Antorchis. From pomacanthids, we describe two new species of Parantorchis, Parantorchis vaili n. sp. and Parantorchis parviovatus n. sp., and two new species of Paradiscogaster, Paradiscogaster hoggettae n. sp. and Paradiscogaster ningalooensis n. sp. We also report Neoparantorchis pomacanthi (Hafeezullah & Siddiqi, 1970) Hafeezullah & Siddiqi, 1971 and Paradiscogaster machidai Cribb, Anderson & Bray, 1999. From ostraciids, we report Discogastroides ostracii (Yamaguti, 1934) Skrjabin & Koval, 1957, Discogastroides macrostomus Shimazu & Kamegai, 1990, and Parayamagutia ostracionis Machida, 1971.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e110"},"PeriodicalIF":2.0,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parasite infection intensity and infection site, rather than host species, shape the histopathological effects of trematode infections in anuran tadpoles. 寄生虫感染的强度和感染部位，而不是宿主物种，塑造了无尾蝌蚪吸虫感染的组织病理学效应。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-21 DOI: 10.1017/S0022149X26102089

M L R Delgado, C da Silva Ribeiro, L A Anjos

{"title":"Parasite infection intensity and infection site, rather than host species, shape the histopathological effects of trematode infections in anuran tadpoles.","authors":"M L R Delgado, C da Silva Ribeiro, L A Anjos","doi":"10.1017/S0022149X26102089","DOIUrl":"https://doi.org/10.1017/S0022149X26102089","url":null,"abstract":"Anuran amphibians play essential ecological roles and are considered important bioindicators. This is especially true during the larval stage, when tadpoles exhibit high sensitivity to parasitic infections, including trematode metacercariae. These infections can profoundly affect the physiology and morphology of juvenile hosts, potentially compromising tissues involved in osmoregulation, locomotion, and development. We analysed naturally infected tadpoles, performing parasitological screening and histopathological evaluation. Through field collection, euthanasia, histological processing, and microscopic analysis of the tissues, our results revealed that the presence of metacercariae is directly associated with progressive tissue damage. In the kidneys, after the establishment of the parasite in a previously healthy renal tubule, we observed alterations ranging from mild changes, such as a slight reduction in glomerular density in individuals with low infection intensity, to severe damage in highly infected individuals. In the tail musculature, the metacercariae were encapsulated between muscle bundles, forming cysts delimited by fibrous capsules, accompanied by alterations in the organisation of muscle fibres, disruption of connective tissue, and the presence of inflammatory infiltrate. Capsules surrounding the parasites were composed of both host tissues and parasite-derived structures. Their formation was frequently associated with necrotic areas and functional impairment. These findings demonstrate that trematode infection exerts progressive effects on the structure and morphology of renal and muscular tissues in tadpoles, with the severity of tissue damage increasing according to parasite infection intensity. Such alterations may compromise host tissue integrity and represent an important pathological consequence of natural trematode infections during the larval stage.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e120"},"PeriodicalIF":2.0,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of crude extracts from different thermally treated Hysterothylacium thalassini L3 larvae on liver pathology and immune responses in C57BL/6 mice - ERRATUM. 不同热处理的海宫草L3幼虫粗提物对C57BL/6小鼠肝脏病理和免疫应答的影响

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-21 DOI: 10.1017/S0022149X26102284

R Abdel-Gaber, M Alotaibi, M Albeshr, M A Dkhil, S Santourlidis, A O Elghamdy, S Al-Quraishy, E M Al-Shaebi

引用次数: 0

Morphological and molecular characterisation of echinostomatid larvae (Digenea: Echinostomatidae) infecting Biomphalaria aff. straminea from Argentina. 阿根廷刺孔虫感染棘孔虫科幼虫的形态和分子特征。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-21 DOI: 10.1017/S0022149X26101680

M V Fernández, M Laidemitt

{"title":"Morphological and molecular characterisation of echinostomatid larvae (Digenea: Echinostomatidae) infecting Biomphalaria aff. straminea from Argentina.","authors":"M V Fernández, M Laidemitt","doi":"10.1017/S0022149X26101680","DOIUrl":"https://doi.org/10.1017/S0022149X26101680","url":null,"abstract":"Echinostomes are a globally distributed group of digeneans with complex life cycles. In South America, many species are known only from definitive hosts, and in Argentina, most larval stages and adult records remain unlinked. Formal morphological descriptions and molecular data are essential for identifying larval stages; however, molecular reference sequences of larval digeneans remain scarce. We present an integrated morphological and molecular characterisation of echinostome cercariae from Biomphalaria aff. straminea collected between January 2017 and February 2018 in a rice field from Corrientes Province, Argentina, based on partial DNA sequences of the nuclear 28S rRNA (28S) gene and the mitochondrial marker nad1 gene. Eighty-three snails (2.12%) of the 3,913 sampled were infected with three echinostomatid morphotypes: Cercaria Echinostoma sp. 20 (1.63%); Cercaria Echinostomatidae gen. sp. 21 (0.25%); and Cercaria Drepanocephalus sp. (0.23%). Available Echinostomatidae sequences in GenBank were aligned with newly generated sequences from echinostomatid cercariae. Phylogenetic analyses showed that Echinostoma sp. 20 represents a distinct lineage that does not group with other Echinostoma sequences in GenBank; Echinostomatidae gen. sp. 21 does not cluster with Echinostoma and likely belongs to a genus related to Patagifer, although its position differs between the 28S and nad1 trees and it shows morphological differences; and Drepanocephalus sp. clusters with D. spathans from Mexico with low genetic divergence (<4.6%), although additional sequence data are needed to clarify species boundaries. This study provides the first molecular identification of Drepanocephalus and Echinostoma species in freshwater snails from Argentina, expanding the molecular dataset for Drepanocephalus in South America.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e121"},"PeriodicalIF":2.0,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can't see the forest for the trees? Statistical considerations for disease macroecology. 只见树木不见森林？疾病宏观生态学的统计考虑。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-18 DOI: 10.1017/S0022149X26101874

A Filion, J-F Doherty

{"title":"Can't see the forest for the trees? Statistical considerations for disease macroecology.","authors":"A Filion, J-F Doherty","doi":"10.1017/S0022149X26101874","DOIUrl":"10.1017/S0022149X26101874","url":null,"abstract":"Disease macroecology relies on large, complex datasets to understand the biotic and abiotic factors shaping parasite distributions and emerging infectious disease risk. These datasets span local to global host-parasite interactions and often integrate diverse host or parasite traits across evolutionary histories. Selecting appropriate statistical approaches requires first asking key questions about the study system: How well is the system understood? How important is predictor accuracy? How much bias is present in the data? We outline three broad analytical pathways commonly used in disease macroecology (frequentist models, Bayesian approaches, and machine learning) and link them to typical data contexts, providing R coding examples using current packages. We emphasise that these pathways are intended as flexible guidelines rather than prescriptive frameworks. Although more advanced approaches have grown in popularity over the last three decades, traditional significance tests remain widely used. While these tests remain appropriate for well-controlled or small-scale experimental systems, they are often unsuitable for complex ecological datasets, where violations of assumptions, hierarchical structure, and sampling biases can lead to unstable or difficult-to-reproduce inferences. We advocate for reporting biologically meaningful predictors, effect sizes, measures of uncertainty, and transparent analytical workflows. By explicitly linking common data challenges in disease macroecology to their consequences for model choice and inference, we encourage more reproducible, transparent, and context-appropriate statistical practices for understanding and forecasting parasite distributions and emerging disease risks.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e109"},"PeriodicalIF":2.0,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Riding South American rivers: a multilocus assessment of Creptotrematina batalhensis (Digenea: Allocreadiidae). 南美河流：巴塔氏隐螺旋体的多位点评估。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-18 DOI: 10.1017/S0022149X26102065

F Gomez, J Barneche, E O Furlan, A Solari, M Ibañez Shimabukuro, G Reig Cardarella, M M Montes

{"title":"Riding South American rivers: a multilocus assessment of Creptotrematina batalhensis (Digenea: Allocreadiidae).","authors":"F Gomez, J Barneche, E O Furlan, A Solari, M Ibañez Shimabukuro, G Reig Cardarella, M M Montes","doi":"10.1017/S0022149X26102065","DOIUrl":"https://doi.org/10.1017/S0022149X26102065","url":null,"abstract":"The Allocreadiidae comprise a diverse group of digeneans infecting freshwater fishes worldwide, with South America harbouring a particularly rich fauna. Within this family, Creptotrematina includes five valid species parasitizing primarily characid fishes, most of which have been recorded from the La Plata Basin. Despite the large geographic extent and hydrological complexity of this basin, the genetic structure and evolutionary relationships of Creptotrematina species across its sub-basins remain poorly understood. The inclusion of a new ITS sequence of Martorellia liciae recovered the same topology observed in the 28S phylogeny, supporting Martorellia as the sister genus of Creptotrematina. In the present study, specimens of Creptotrematina spp. collected in the Paraná and Iguazú rivers were analysed using partial sequences of 28S rDNA, ITS, and mitochondrial COI genes. Phylogenetic analyses based on individual markers recovered the new material within Creptotrematina batalhensis with strong support. Genetic divergence between Argentine and Brazilian populations of C. batalhensis was extremely low or absent across all markers, despite the large geographic distances and differences in hosts infected. These results indicate basin-wide genealogical cohesion and the absence of detectable phylogeographic structure. The observed pattern suggests that hydrographic subdivision within the La Plata Basin has not acted as an effective evolutionary barrier for this species. Instead, the strong association of Creptotrematina spp. with closely related characid hosts supports the hypothesis that host phylogeny and host-mediated dispersal may play a central role in shaping parasite diversification, with hydrological dynamics modulating connectivity rather than driving lineage fragmentation.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e114"},"PeriodicalIF":2.0,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new genus for the overlooked Creptotrema funduli (Digenea: Allocreadiidae) based on morphological and molecular evidence. 基于形态学和分子证据的被忽视的眼底Creptotrema新属（digenia: allocrecreadidae）。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-18 DOI: 10.1017/S0022149X26101904

M M Montes, G Reig Cardarella

{"title":"A new genus for the overlooked Creptotrema funduli (Digenea: Allocreadiidae) based on morphological and molecular evidence.","authors":"M M Montes, G Reig Cardarella","doi":"10.1017/S0022149X26101904","DOIUrl":"https://doi.org/10.1017/S0022149X26101904","url":null,"abstract":"Creptotrema funduli Mueller, 1934, has long presented an uncertain systematic position within the family Allocreadiidae. Previous phylogenetic analyses based on partial 28S rDNA sequences recovered this species as distinct from other members of Creptotrema, suggesting that its current generic placement may not reflect its evolutionary relationships. In the present study, we re-evaluate the taxonomic status of C. funduli through an integrative approach combining morphological observations and phylogenetic analyses based on 28S rDNA sequences. Our results consistently recover C. funduli outside the clade containing species currently assigned to Creptotrema. In addition, several morphological traits distinguish this species from congeners, supporting its recognition as a separate evolutionary lineage within Allocreadiidae. Based on these findings, we propose a new genus to accommodate C. funduli. The new genus is compared with closely related genera within Allocreadiidae, particularly those with one pair of oral lobes like Creptotrema. Our results highlight the need for continued taxonomic revision within the family and illustrate the value of integrating molecular and morphological evidence in resolving the systematics of allocreadiid trematodes.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e117"},"PeriodicalIF":2.0,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Steinernema hattingi n. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from South Africa. 南非斯坦奈马·哈廷吉·sp.一种昆虫病原性线虫新种（线虫纲：斯坦奈马科）。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-18 DOI: 10.1017/S0022149X26101837

V Půža, H Çimen, J Nermuť, X Wang, S H Gulsen, T Ramakuwela, S Hazir

{"title":"Steinernema hattingi n. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from South Africa.","authors":"V Půža, H Çimen, J Nermuť, X Wang, S H Gulsen, T Ramakuwela, S Hazir","doi":"10.1017/S0022149X26101837","DOIUrl":"https://doi.org/10.1017/S0022149X26101837","url":null,"abstract":"A new entomopathogenic nematode, Steinernema hattingi n. sp., was isolated during a survey in Mpumalanga Province of South Africa. Morphological and morphometric traits place the species in the glaseri-group, characterised by large infective juveniles (IJs) with average body length exceeding 1000 μm. The IJs of S. hattingi n. sp. have a mean body length of 1059 μm, lateral fields composed of eight equally spaced ridges (nine incisures) at mid-body (formula 2, 6, 8, 4, 2), a relatively short tail (c ratio 16 on average) with a hyaline region occupying approximately 45% of its length, and are relatively slender (a ratio 33 on average). Male spicules are curved, brown, with a pointed tip and an elongate manubrium (length/width ratio 1.8:1); male tails are non-mucronate or occasionally with a short papilla-like mucron in second-generation males. First-generation females have a moderately protruding, slightly asymmetrical vulva without postanal swelling, whereas second-generation females display a protruding, asymmetrical vulva and a slightly developed postanal swelling. The species is further characterised by sequences of the internal transcribed spacer (ITS) and partial 28S rDNA (D2-D3) regions. Phylogenetic analyses place S. hattingi n. sp. within the Khoisanae clade of the glaseri-group.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e116"},"PeriodicalIF":2.0,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0