M Amiri Bonab, M R Atighi, R Gholami Ghavamabad, M Pedram
{"title":"First report of <i>Oscheius insectivorus</i> (Körner, 1954) Andrássy, 1976 (Nematoda: Rhabditidae) from Iran with its efficacy against <i>Chrysomela populi</i> L. (Col.: Chrysomelidae) and <i>Galleria mellonella</i> L. (Lep.: Pyralidae).","authors":"M Amiri Bonab, M R Atighi, R Gholami Ghavamabad, M Pedram","doi":"10.1017/S0022149X25000070","DOIUrl":"https://doi.org/10.1017/S0022149X25000070","url":null,"abstract":"<p><p>A population of <i>Oscheius insectivorus</i> was recovered from inside the body of a live scarab beetle collected from natural forests of Jafar Abad village, Golestan province, and was reported from Iran for the first time. The Iranian isolate of the species is characterized by 1103-1942 μm long females with their vulva at 46.9-57.4% of the body, tail 75-118 μm long, males with spicules 60.0-74.5 μm long, gubernaculum 23.7-32.2 μm long, and infective juveniles measuring 790-985 μm long. Females and males of the recovered population had shorter body when compared with the type population. Molecular identification of the recovered population was performed using the SSU and D2-D3 expansion segments of LSU rDNA, and corresponding phylogenetic trees were reconstructed and discussed. The pathogenicity of the Iranian isolate was evaluated on the larvae and adults of the poplar leaf beetle, <i>Chrysomela populi</i>, and the larvae of the wax moth, <i>Galleria mellonella</i>, under laboratory conditions. A suspension of 200 infective juveniles per ml (IJs/ml) of the nematode was most effective against third instar larvae of <i>C. populi</i>, causing 75% mortality 72 hours post-exposure. In adults, mortality reached 70% at the same concentration and exposure time. For <i>G. mellonella</i> larvae, <i>O. insectivorus</i> caused 50% mortality at 200 IJs/ml within 72 hours post-exposure. The lethal concentration 50 (LC50) values of the nematode were 77.71 and 94.06 IJs/ml for third instar larvae and adults of <i>C. populi</i>, respectively, and 217.04 IJs/ml for fifth instar larvae of <i>G. mellonella</i> 72 hours post-exposure at 25°C and 60% relative humidity.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e36"},"PeriodicalIF":1.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K V Galaktionov, A Gonchar, K M Wegner, R Wolfensberger, C Buschbaum, A E Romanovich
{"title":"Life cycle of the seabird digenean <i>Gymnophallus minor</i> (Gymnophallidae) in the Arctic.","authors":"K V Galaktionov, A Gonchar, K M Wegner, R Wolfensberger, C Buschbaum, A E Romanovich","doi":"10.1017/S0022149X25000173","DOIUrl":"https://doi.org/10.1017/S0022149X25000173","url":null,"abstract":"<p><p>Gymnophallidae is one of the digenean families featuring bivalves as first intermediate hosts. However, the exact bivalve host species remain unknown for most members of this family. Gymnophallids have been one of the targets in our continuous efforts to reveal the diversity of digeneans in the higher north. Here, we focus on <i>Gymnophallus minor</i>, which we found in eiders from various locations in the Arctic and sub-Arctic. Sexual adults (maritae) of <i>G. minor</i> can be easily identified because they have a distinctive character: the roughly equal size of the pharynx and the ventral sucker. We also matched them, using DNA markers, with the intramolluscan stages (sporocysts, cercariae, and metacercariae) from the bivalve <i>Liocyma fluctuosa</i> collected on Spitsbergen. Taken together, we compile the first data on the life cycle of <i>G. minor</i> and discuss them in the context of other gymnophallids.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e37"},"PeriodicalIF":1.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R Svitin, O Marushchak, I Dmytriieva, V Dupak, O Greben, A Nechai, Y Syrota
{"title":"Helminths of the rock lizards <i>Darevskia dahli</i> and <i>D. armeniaca</i> in their invaded range in Ukraine.","authors":"R Svitin, O Marushchak, I Dmytriieva, V Dupak, O Greben, A Nechai, Y Syrota","doi":"10.1017/S0022149X25000203","DOIUrl":"https://doi.org/10.1017/S0022149X25000203","url":null,"abstract":"<p><p>This study investigated the helminths of the mixed invasive population of <i>Darevskia armeniaca</i> and <i>D. dahli</i>, collected during two field trips in Denyshy, Zhytomyr region, Ukraine, in 2023. In total, 67 adult lizards (35 <i>D. armeniaca</i> and 32 <i>D. dahli</i>) were examined. Molecular and morphological approaches were used to identify the parasites. The analyses revealed six helminth species, including four nematodes (<i>Toxocara cati</i>, <i>Strongyloides darevskyi, Oswaldocruzia</i> sp., and Spirurida gen. sp.), one trematode (<i>Pleurogenes claviger</i>), and one cestode (<i>Mesocestoides litteratus</i>). <i>Toxocara cati</i> had the highest prevalence, found in cysts located primarily on the liver and in the body cavity of the hosts. The qualitative and quantitative comparative assessment of the helminth community suggests that, due to the introduction of these lizards, most helminth species from their native range have been lost. Additionally, most local helminth species have not yet adapted to parasitising these lizards as normal hosts of their life cycle.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e38"},"PeriodicalIF":1.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N V Leiva, D Montenegro, C Castro, M Silva, R Vidal, M T González
{"title":"Is parasitic infection a buffer against metal pollution?","authors":"N V Leiva, D Montenegro, C Castro, M Silva, R Vidal, M T González","doi":"10.1017/S0022149X2500015X","DOIUrl":"https://doi.org/10.1017/S0022149X2500015X","url":null,"abstract":"<p><p>Metal pollution is a major global issue in aquatic environments, affecting environmental quality and potentially altering host-parasite dynamics. This study evaluates the buffering role of a larval trematode <i>Himasthla</i> sp. under experimental conditions to test the effect of copper (Cu) exposure on the survival of the marine snail <i>Echinolittorina peruviana.</i> Snails were collected from intertidal rocky pools over a two-month period from Coloso (23°45'S, 70°28'W), northern Chile, and identified as parasitized or unparasitized. Both groups were then exposed to Cu concentrations (3 and 6 mg/L). Kaplan-Meier curves were used to determine the percentage of survival over time and the respective confidence intervals (CI). A nested ANOVA was conducted to assess whether rediae abundance per snail varied by experiment time, snail status, and Cu concentration. Snail survival was affected by both Cu-concentrations, but the effect was greater at 6 mg/L. At 3 mg/L, 57% (CI: 49.9-66.6%) of unparasitized snails were alive at 192 h, while 56% (CI: 46.6-67.4%) of parasitized snails survived at 216 h. At 6 mg/L, 42% (CI:35-51%) of unparasitized snails survived at 192 h, while 48% of parasitized snails survived at 216 h (CI:39-59%). Regardless of Cu concentration, after 240 h, all unparasitized snails had died, while 15% of parasitized snails remained alive. Dead snails harboured 125±53 rediae, while survivors had 194±73 rediae, with no significant differences between treatments. Our results show that parasitized snails survived longer than unparasitized snails, suggesting a trade-off between parasitism and host survival in polluted environments.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e34"},"PeriodicalIF":1.3,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S Mohtasebi, S Ahn, B Rosa, K Moyes, T A Kuzmina, J S Gilleard, J Poissant
{"title":"'First record of <i>Hsiungia pekingensis</i> (Nematoda: Strongylidae) in North America: Morphological and molecular identification of a rare equine strongyle.","authors":"S Mohtasebi, S Ahn, B Rosa, K Moyes, T A Kuzmina, J S Gilleard, J Poissant","doi":"10.1017/S0022149X25000185","DOIUrl":"https://doi.org/10.1017/S0022149X25000185","url":null,"abstract":"<p><p>Equids are infected by a diversity of gastrointestinal nematode parasites, including 64 species of equine strongyle nematodes from19 genera. Despite numerous surveys of horse strongyles worldwide, certain geographic regions and rare species remain understudied. In 1964, a new species of equine strongyle, <i>Cylicocyclus pekingensis</i>, was described from a donkey in China. Subsequently, this species was recorded in horses from Kazakhstan and reclassified as <i>Hsiungia pekingensis</i> (K'ung and Yang, 1964), the only species in this genus. Since then, <i>H. pekingensis</i> has not been reported elsewhere, with limited knowledge on its distribution and phylogeny.This study documents the first record of <i>H. pekingensis</i> in North America. Adult specimens were recovered from fecal samples of a domestic horse in Alberta, Canada, following treatment with ivermectin. Species identification involved detailed morphological examination, complemented with sequencing of the internal transcribed spacer 1 (ITS1), 5.8S rRNA gene, and the internal transcribed spacer 2 (ITS2) regions of the nuclear genome. Phylogenetic analysis indicated a close evolutionary relationship with species from <i>Poteriostomum</i> and <i>Parapoteriostomum</i> genera. Nemabiome ITS2 sequencing of a paired pre-treatment sample also detected the presence of <i>H. pekingensis</i> in the studied horse. Re-analysis of public equine nemabiome datasets further detected <i>H. pekingensis</i> in feral horses in Alberta, but not in other regions considered. This study expands the known distribution of this rare species and enhances our knowledge of its placement in the phylogeny of equine strongyles. Furthermore, our re-analysis of public nemabiome datasets highlights the value of this approach for studying the global distribution of parasite species.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e35"},"PeriodicalIF":1.3,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Faltýnková, O Kudlai, D Jouet, K O'Dwyer, C Pantoja, K Skírnisson
{"title":"Freshwater trematodes in Iceland and the surrounding north - current advances and questions.","authors":"A Faltýnková, O Kudlai, D Jouet, K O'Dwyer, C Pantoja, K Skírnisson","doi":"10.1017/S0022149X25000215","DOIUrl":"https://doi.org/10.1017/S0022149X25000215","url":null,"abstract":"<p><p>Iceland is an isolated, sub-Arctic, oceanic island of volcanic origin in the northern North Atlantic. With a limited faunal diversity and being the most northern point in the distributional range for some species, it is an intriguing model region to study parasite biodiversity and biogeography. Since 2006, there has been a history of intense biodiversity discoveries of freshwater trematodes (Trematoda, Digenea), thanks to the use of integrative taxonomic methods. The majority of digeneans (28 out of 41 known) were characterised with molecular genetic methods and morphological analyses, with some of their life-cycle stages and geographical distribution assessed. A surprising diversity has been discovered, comprising species of the families Allocreadiidae, Cyclocoeliidae, Diplostomidae, Echinostomatidae, Gorgoderidae, Plagiorchiidae, Notocotylidae, Schistosomatidae, and Strigeidae. Many of the recorded species complete their life cycles within Iceland, with three snail species (<i>Ampullaceana balthica</i>, <i>Gyraulus parvus</i>, <i>Physa acuta</i>) known as intermediate hosts. No trematodes endemic for Iceland were found; they appear to be generalists with wide geographical ranges dispersed mainly by migratory birds. Interestingly, fish trematodes recorded in Iceland were found in mainland Europe, indicating that they might be dispersed by anadromous fishes, by human activity, or by migratory birds carrying intermediate hosts. The trematode fauna is mainly Palaearctic, with few species recorded in North America. We highlight the ongoing need for precise species identification via integrative taxonomic methods, which is a baseline for any further ecological studies and adequate epidemiological and conservation measures. Also, there is still a need of obtaining well-preserved vouchers of adults for definite species delimitation.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e33"},"PeriodicalIF":1.3,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
W T Hasuike, B Scorsim, I S Arjona, R B Amaral, L Damacena-Silva, G A Araújo, S Bellay, A V de Oliveira, R M Takemoto
{"title":"Morphology and molecular characterization of a new species of <i>Tereancistrum</i> parasite from the gills of <i>Brycon nattereri</i>.","authors":"W T Hasuike, B Scorsim, I S Arjona, R B Amaral, L Damacena-Silva, G A Araújo, S Bellay, A V de Oliveira, R M Takemoto","doi":"10.1017/S0022149X25000148","DOIUrl":"https://doi.org/10.1017/S0022149X25000148","url":null,"abstract":"<p><p>A new species of <i>Tereancistrum</i> Kritsky, Thatcher & Kayton, 1980, collected from the gills of <i>Brycon nattereri</i> (Bryconidae) in the Cerrado, in the state of Goiás, Brazil, is described based on morphological and molecular data, based on the mitochondrial cytochrome <i>c</i> oxidase subunit 1 (<i>COI</i>) gene region. <i>Tereancistrum campanum</i> n. sp. is distinguished from all its congeners by the unique 'bell-shaped' accessory piece in the male copulatory complex. Molecular analysis revealed that the specimens analyzed formed a distinct group from other <i>Tereancistrum</i> sequences and that the sequences of <i>Tereancistrum kerri</i> showed genetic divergence when compared to <i>T. campanum</i> n. sp., confirming the molecular distinction between the two species. This study also expands the knowledge of parasitic diversity in the Tocantins-Araguaia River Basin and extends the geographical distribution of the genus <i>Tereancistrum.</i> The combination of morphological and genetic data was essential for delimiting this new species, highlighting the importance of integrative approaches in understanding the diversity of monogeneans in freshwater fish in Brazil.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e32"},"PeriodicalIF":1.3,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First report of a dactylogyrid, <i>Cichlidogyrus sclerosus</i> Paperna & Thurston, 1969 (Monogenoidea) infecting Nile tilapia, <i>Oreochromis niloticus</i> (Linnaeus, 1758) (Cichliformes: Cichlidae) in the United States, with a review of host and locality records in its invasive range and a phylogenetic analysis.","authors":"J H Brule, M B Warren, S A Bullard","doi":"10.1017/S0022149X24000919","DOIUrl":"https://doi.org/10.1017/S0022149X24000919","url":null,"abstract":"<p><p>The parasites of Nile tilapia, <i>Oreochromis niloticus</i> (Linnaeus, 1758) are poorly documented in the United States despite the economic importance and global introduction of this African fish. Only one metazoan parasite (<i>Gyrodactylus cichlidarum</i> Paperna, 1968; Gyrodactylidae) reportedly infects Nile tilapia in the United States. Examining Nile tilapia from a flow-through aquaculture system hydrologically linked to Sougahatchee Creek (Tallapoosa River, Auburn, Alabama), we observed a gill infection by <i>Cichlidogyrus sclerosus</i> Paperna & Thurston, 1969 (Dactylogyridae). This monogenoid was originally described from the gill of Mozambique tilapia, <i>Oreochromis mossambicus</i> (Peters, 1852) from Lake Victoria, Uganda. Specimens of <i>C. sclerosus</i> were studied for morphology and phylogenetic analyses using the <i>28S</i> and <i>ITS1.</i> We identified our specimens as <i>C. sclerosus</i> because they had the following combination of morphological features: marginal hooks shorter than dorsal anchor length; anchor roots reduced; dorsal anchor point bent; dorsal bar pyriform projections approximately half as long as dorsal bar width; penis short (<100 μm), not coiled, tubular, lacking swelling, having irregularly surfaced heel; and accessory piece straight and bifid. Our <i>28S</i> and <i>ITS1</i> phylogenies recovered our <i>C. sclerosus</i> sequences in a clade with conspecific sequences and showed no obvious biogeographic pattern. <i>Cichlidogyrus sclerosus</i> reportedly infects 21 fishes of 11 genera and 3 families from 36 countries in Africa, Asia, North America, South America, and Europe. The study of Nile tilapia parasites, especially those exhibiting direct life cycles and low host specificity, is important because they comprise potential invasive species.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e17"},"PeriodicalIF":1.3,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L Franceschini, F G C Dias, B S Miguel, A C Zago, M L Orsi, J R S Vitule, F S de Almeida, F Porto-Foresti, J A Balbuena, I P Ramos, L Casatti
{"title":"Eye metacercariae in invasive pirarucu <i>Arapaima gigas</i> (Pisces: Arapaimidae): A potential ecological sink interaction?","authors":"L Franceschini, F G C Dias, B S Miguel, A C Zago, M L Orsi, J R S Vitule, F S de Almeida, F Porto-Foresti, J A Balbuena, I P Ramos, L Casatti","doi":"10.1017/S0022149X25000045","DOIUrl":"10.1017/S0022149X25000045","url":null,"abstract":"<p><p>Biological invasions are among the main threats to global biodiversity and present the potential to disrupt host-parasite dynamics. In Brazil, the scientific reports of the occurrence of the Amazonian fish 'pirarucu' (<i>Arapaima gigas</i>) in the upper Paraná River basin, São Paulo state, were made in 2015. However, the effects of its introduction are still unknown, including those associated to its host-parasite relationships. As part of our studies on the possible effects of <i>A. gigas</i> introduction into this basin, the parasites from the eyes of 60 specimens of <i>A. gigas</i> were evaluated. We reported the occurrence of <i>Austrodiplostomum compactum</i> metacercariae (Trematoda, Diplostomidae) parasitizing the eyes of <i>A. gigas</i> supported by morphological and molecular data (COI mtDNA). The new partial sequences had a similarity of 100% to other sequences of <i>Au. compactum</i> previously deposited in Genbank. Five hosts (Prevalence = 8.33%) with a standard length between 69 and 116 cm were infected, with a mean intensity of infection of 31.8 ± 17.2 (1-93). Large infected hosts are unlikely to be a significant prey item for the definitive hosts (medium-sized piscivorous birds), and could act as an 'ecological sink', disrupting the transmission of this metacercariae. Hosts with high infection rates by these metacercariae may present cataracts, exophthalmos, and blindness, resulting in loss of visual acuity. Considering that <i>A. gigas</i> is a carnivorous and visual predator, the low infection observed possibly does not act as a biological filter to hinder or contain the invasion, probably presenting a low negative effect on the visual acuity of hosts.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e30"},"PeriodicalIF":1.3,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K M Anes, J Patil, M Babu, N Aarthi, B S Gotyal, A Josephrajkumar, P H Mhatre, J V Sajan, M T Gowda, V Půža
{"title":"<i>Steinernema keralense</i> n. sp., a new entomopathogenic nematode (Nematoda: Steinernematidae) from India.","authors":"K M Anes, J Patil, M Babu, N Aarthi, B S Gotyal, A Josephrajkumar, P H Mhatre, J V Sajan, M T Gowda, V Půža","doi":"10.1017/S0022149X25000136","DOIUrl":"https://doi.org/10.1017/S0022149X25000136","url":null,"abstract":"<p><p>In this study, morphological and molecular features were used to identify a new <i>Steinernema</i> sp. from Kerala, India. Morphological and molecular features provide evidence for placing the new species into the <i>longicaudum</i> clade. The new species is characterized by the following morphological features: infective juveniles with a body length of 1067 μm (914-1268 μm); a distance from the anterior end to excretory pore of 82 μm (73-92 μm); a distance from anterior end to nerve ring of 105 μm (91-118 μm). The distinguishing feature of the infective juveniles of <i>S. keralense</i> n. sp. is the presence of seven ridges in the mid-body region, while all other species classified within the <i>logicaudum</i> clade to date are characterized by eight ridges. The first-generation males are characterised by 25 genital papillae, very short spicules, with a length of 68 μm (60-72 μm), and the SW% ratio is 136 (114-169). The new species is further characterized by sequences of the internal transcribed spacer and partial 28S regions of the ribosomal DNA. Phylogenetic analyses show that <i>S. keralense</i> n. sp. is closely related to species within the <i>longicaudum</i> clade.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e31"},"PeriodicalIF":1.3,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}