Journal of Helminthology最新文献_第2页

The impact of ivermectin-loaded solid lipid nanoparticles on the enteric phase of experimental trichinellosis. 负载伊维菌素的固体脂质纳米颗粒对实验性旋毛虫病肠期的影响。

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-04-22 DOI: 10.1017/S0022149X2500029X

S K Hammad, M H Almotayam, A S N Mohamed, T I Farag

{"title":"The impact of ivermectin-loaded solid lipid nanoparticles on the enteric phase of experimental trichinellosis.","authors":"S K Hammad, M H Almotayam, A S N Mohamed, T I Farag","doi":"10.1017/S0022149X2500029X","DOIUrl":"https://doi.org/10.1017/S0022149X2500029X","url":null,"abstract":"Trichinellosis is a global zoonotic disease affecting humans and nearly all animal species. The intestinal (enteric) phase of trichinellosis is critical, as it determines the course and prognosis of the disease. The medications used in the management of trichinellosis demonstrate inadequate bioavailability, along with a significant level of resistance. Therefore, there is a need for the development of novel agents that enhance the bioavailability of administered medications. Nanobiotechnology has emerged as a significant strategy in treating parasitic diseases. This study examined the use of solid lipid nanoparticles (SLNs) to improve the efficacy of oral ivermectin (IVM) in treating the enteric phase of trichinellosis. Thirty-five Swiss albino mice were divided into seven equal groups as follows: negative control, positive control, albendazole, ivermectin, SLNs, ivermectin loaded on solid lipid nanoparticles (IVM-SLNs), and a combination of IVM-SLNs and albendazole. Mice were sacrificed on the seventh day post-infection. The drugs' effects were assessed using parasitological, biochemical, histological, histochemical and immunohistochemical analyses. The co-administration of albendazole and IVM-SLNs resulted in a significant decrease in adult burden, inflammatory cell infiltration, and apoptosis. Furthermore, a significant reduction in Cyclooxygenase-2 (COX-2) expression was observed compared to the infected untreated control group, along with improved liver and kidney function indices. In conclusion, the potent trichinocidal effect of a single oral dose of IVM-SLNs against Trichinella adults makes them a promising alternative or adjunct to existing nematicidal agents.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e53"},"PeriodicalIF":1.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Challenges in the recognition of trematode species: Consideration of hypotheses in an inexact science. 识别吸虫物种的挑战：对不精确科学中的假设的考虑。

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-04-22 DOI: 10.1017/S0022149X25000367

T H Cribb, D P Barton, D Blair, N J Bott, R A Bray, R D Corner, S C Cutmore, M L I De Silva, B Duong, A Faltýnková, A Gonchar, R F Hechinger, K K Herrmann, D C Huston, P T J Johnson, G Kremnev, R Kuchta, C Louvard, W J Luus-Powell, S B Martin, T L Miller, G Pérez-Ponce de León, N J Smit, V V Tkach, M Truter, T Waki, A Vermaak, N Q-X Wee, R Q-Y Yong, T J Achatz

{"title":"Challenges in the recognition of trematode species: Consideration of hypotheses in an inexact science.","authors":"T H Cribb, D P Barton, D Blair, N J Bott, R A Bray, R D Corner, S C Cutmore, M L I De Silva, B Duong, A Faltýnková, A Gonchar, R F Hechinger, K K Herrmann, D C Huston, P T J Johnson, G Kremnev, R Kuchta, C Louvard, W J Luus-Powell, S B Martin, T L Miller, G Pérez-Ponce de León, N J Smit, V V Tkach, M Truter, T Waki, A Vermaak, N Q-X Wee, R Q-Y Yong, T J Achatz","doi":"10.1017/S0022149X25000367","DOIUrl":"https://doi.org/10.1017/S0022149X25000367","url":null,"abstract":"The description and delineation of trematode species is a major ongoing task. Across the field there has been, and currently still is, great variation in the standard of this work and in the sophistication of the proposal of taxonomic hypotheses. Although most species are relatively unambiguously distinct from their congeners, many are either morphologically very similar, including the major and rapidly growing component of cryptic species, or are highly variable morphologically despite little to no molecular variation for standard DNA markers. Here we review challenges in species delineation in the context provided to us by the historical literature, and the use of morphological, geographical, host, and molecular data. We observe that there are potential challenges associated with all these information sources. As a result, we encourage careful proposal of taxonomic hypotheses with consideration for underlying species concepts and frank acknowledgement of weaknesses or conflict in the data. It seems clear that there is no single source of data that provides a wholly reliable answer to our taxonomic challenges but that nuanced consideration of information from multiple sources (the 'integrated approach') provides the best possibility of developing hypotheses that will stand the test of time.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e54"},"PeriodicalIF":1.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Far from home: Morphological, ultrastructural, and molecular characterization of Thometrema patagonicum (Szidat, 1956) (Hemiuroidea: Derogenidae) from an unexpected host and locality, with taxonomic implications. 远离家乡：来自一个意想不到的寄主和地点的巴塔哥尼亚托米特雷马的形态学、超微结构和分子特征（Szidat, 1956）（半蝇总科：脱毛蝇科），具有分类意义。

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-04-14 DOI: 10.1017/S0022149X25000355

P V Alves, M B Ebert, Reinaldo R J Silva

{"title":"Far from home: Morphological, ultrastructural, and molecular characterization of Thometrema patagonicum (Szidat, 1956) (Hemiuroidea: Derogenidae) from an unexpected host and locality, with taxonomic implications.","authors":"P V Alves, M B Ebert, Reinaldo R J Silva","doi":"10.1017/S0022149X25000355","DOIUrl":"10.1017/S0022149X25000355","url":null,"abstract":"While most higher taxa in the Hemiuroidea exhibit remarkable diversity and thrive worldwide in marine ecosystems, a few groups have successfully radiated into freshwater environments. Among them, species of Thometrema are well known from freshwater and brackish fishes in the New World. In this study, specimens of Thometrema from a single specimen of the endangered pimelodid catfish Steindachneridion scriptum in the Upper Paraná River basin, State of São Paulo, Brazil, were subjected to morphological, ultrastructural (first scanning electron microscopy data for the genus) and molecular analyses. Newly obtained sequences of the partial large subunit nuclear ribosomal RNA (28S rDNA) and partial mitochondrial cytochrome c oxidase subunit I (COI mtDNA) provided strong evidence supporting the identification of the specimens as T. patagonicum, a trematode previously known only from endemic percichthyid fishes (Percichthyidae) in the Argentinian Patagonia. This unexpected finding, along with critical morphological evaluation of new and deposited material, led us to propose T. overstreeti and T. bonariense as junior synonyms of T. patagonicum. Moreover, we reassessed certain morphological features, revealed new potential distinguishing characters (papillae) and raised doubts about the taxonomic value of commonly used features for species differentiation (e.g., vitellarium masses). To overcome taxonomic problems in Thometrema due to the paucity of morphological features to clearly set species boundaries, new descriptions could leverage integrative approaches, as employed here, to establish robust species hypotheses and ultimately unravel the actual species composition in the Americas.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e52"},"PeriodicalIF":1.3,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144020680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantifying senescence, death rates, and lifespans of trematode parthenitae. 量化孤雌吸虫的衰老、死亡率和寿命。

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-04-14 DOI: 10.1017/S0022149X25000331

D C G Metz, E M Palmer, R F Hechinger

{"title":"Quantifying senescence, death rates, and lifespans of trematode parthenitae.","authors":"D C G Metz, E M Palmer, R F Hechinger","doi":"10.1017/S0022149X25000331","DOIUrl":"https://doi.org/10.1017/S0022149X25000331","url":null,"abstract":"For many trematode species, individual reproductive parthenitae in first intermediate host colonies senesce, die, and are replaced by newly born parthenitae. The times involved in these processes are poorly understood. Here, we present an approach to estimate parthenita death rates and lifespans that uses readily obtainable data on senescent parthenita frequencies, brood sizes, and offspring (cercaria) release rates. The onset of parthenita senescence is often marked by the degeneration and disappearance of the germinal mass, its source of new offspring. Following germinal mass loss, the remaining viable offspring in a senescent parthenita finish development and are birthed before parthenita death. Therefore, a senescing parthenita's remaining lifespan is the time it takes for all its viable offspring to mature and exit. We can estimate this time by measuring whole-colony (infected snail) cercaria shed rates, dissecting colonies to count reproductives, and then apply the per redia cercaria production rate to the observed brood sizes of senescent parthenitae. The per-capita parthenita death rate is then calculated as the proportion of parthenitae that are senescent divided by their average remaining lifespan. Reproductive parthenita lifespan is the inverse of this death rate. We demonstrate the approach using philophthalmid trematodes, first providing documentation of a free-floating germinal mass in 4 philophthalmids, and then, for 3 of those species, estimating parthenita senescence rates, death rates, and lifespans. This method should be broadly applicable among trematode species and help inform our understanding of trematode colony dynamics, social structure, and the evolution of parthenita senescence.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e51"},"PeriodicalIF":1.3,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First detection of Psilotrema limosum in muskrat in East Asia and a description of Psilotrema elegans n. sp. 东亚麝鼠中首次检出灰霉病螨及线虫描述。

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-04-10 DOI: 10.1017/S0022149X25000318

K A Kalinina, Yu V Tatonova, V V Besprozvannykh, M Yu Shchelkanov

{"title":"First detection of Psilotrema limosum in muskrat in East Asia and a description of Psilotrema elegans n. sp.","authors":"K A Kalinina, Yu V Tatonova, V V Besprozvannykh, M Yu Shchelkanov","doi":"10.1017/S0022149X25000318","DOIUrl":"https://doi.org/10.1017/S0022149X25000318","url":null,"abstract":"The East Asian region has seen the first discovery of trematodes of the genus Psilotrema in the muskrat Ondatra zibethicus. When studying the morphology of the trematodes, two morphotypes were identified among individuals of Psilotrema spp., the differences between which at the morphological level are equivalent to those between species. The affiliation of one of these morphotypes with the bird parasite Psilotrema limosum was established based on partial sequences of genes coding for 28S ribosomal RNA, nad1, and cox1 mitochondrial DNA. Based on the analysis of these three markers, we classified individuals with this morphotype as P. limosum, despite some morphological features of worms infecting birds. At the same time, the worms with second morphotype had no interspecies differences based on the first two markers, while the cox1 gene confirmed the separation of these samples on a species level. These trematodes were described as a new species, Psilotrema elegans n. sp., based on both morphological and molecular data.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e50"},"PeriodicalIF":1.3,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144029375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological variety and mechanisms of formation of acanthocephalan cysts in intermediate host. 中间寄主棘头虫包囊的形态变化及其形成机制。

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-03-31 DOI: 10.1017/S0022149X25000306

V P Nikishin, T V Davydenko, E M Skorobrekhova

{"title":"Morphological variety and mechanisms of formation of acanthocephalan cysts in intermediate host.","authors":"V P Nikishin, T V Davydenko, E M Skorobrekhova","doi":"10.1017/S0022149X25000306","DOIUrl":"10.1017/S0022149X25000306","url":null,"abstract":"This report provides an overview of available data on the ultrathin structure and the mechanisms of formation of cysts around acanthocephalans of the classes Palaeacanthocephala and Archiacanthocephala in intermediate hosts. In most of the described cases, the cyst is bi-layered, consisting of an outer layer that is relatively thick and vesicular, and an underlying layer that is thin and amorphous. In acanthellae of some species, a lamellar layer is observed on the cyst surface, which degrades presumably at the cystacanth stage. Three probable mechanisms of cyst formation in acanthellae of different species have been identified. The first and second mechanisms involve the border of microvilli that develops on the tegument surface. In acanthellae of the first group, the cyst's vesicular layer is formed by detachment of fragments or small vesicles from microvilli, a process resembling the micro-apocrine secretion, while the amorphous layer is composed of electron-dense material released from invaginates of the tegument's outer membrane and the remaining microvilli. In acanthellae of the second group, the cyst is single-layered and formed presumably of whole microvilli separated from the tegument. Since acanthellae of the third group lack microvilli on the tegument surface, the vesicular layer of the cyst is formed of a few small vesicles and amorphous material synthesized deep in the tegument and in large vesicles transported to the parasite's surface. The amorphous, electron-dense layer of the cyst is formed in the same manner as in the first group, but without microvilli.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e49"},"PeriodicalIF":1.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143752837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scleroductus (Platyhelminthes: Monogenea) infecting Rhamdia spp. (Siluriformes: Heptapteridae) in Mexico, and their phylogenetic position within Gyrodactylidea. 墨西哥Rhamdia spp. （siluriforma: Heptapteridae）感染的Scleroductus （platymintes: Monogenea）及其在gyrodactyae中的系统发育地位。

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-03-13 DOI: 10.1017/S0022149X25000264

A García-Vásquez, C D Pinacho-Pinacho, I Guzmán-Valdivieso, M Calixto-Rojas, D A Pozos-Carré, M Rubio-Godoy

{"title":"Scleroductus (Platyhelminthes: Monogenea) infecting Rhamdia spp. (Siluriformes: Heptapteridae) in Mexico, and their phylogenetic position within Gyrodactylidea.","authors":"A García-Vásquez, C D Pinacho-Pinacho, I Guzmán-Valdivieso, M Calixto-Rojas, D A Pozos-Carré, M Rubio-Godoy","doi":"10.1017/S0022149X25000264","DOIUrl":"10.1017/S0022149X25000264","url":null,"abstract":"Gyrodactylidae Cobbold, 1864 is a monophyletic family of hyperviviparous species, with 36 genera and approximately 700 species. Here, we focused on one of these genera - namely Scleroductus Jara & Cone, 1989 - characterizing its morphological variation systematically and testing its phylogenetic position in relation to other genera in the family. We collected and describe two new species of monogenean parasites of Scleroductus infecting Neotropical freshwater catfishes in Mexico. Scleroductus veracruzano n. sp. infects Guatemalan chulín, Rhamdia guatemalensis in Veracruz, while Scleroductus oaxaqueno n. sp. infects filespine chulín, Rhamdia laticauda in Oaxaca, Mexico. Sequences of the internal transcribed spacer (ITS rDNA) and 18S rDNA were generated for both species. Phylogenetic hypotheses based on maximum likelihood and Bayesian inference analyses of 18S rDNA indicate that the two new Mexican taxa are closely related to each other, and both appear as sister species to an undescribed but molecularly characterized Scleroductus sp. infecting South American catfish, Rhamdia quelen in Brazil. Our analyses confirm the placement of Scleroductus spp. within the monophyletic family Gyrodactylidae, whose sister clade is the Oogyrodactylidae. Within Gyrodactylidae, Scleroductus spp. form a clade sister to a well-supported clade composed by members of the genera Ieredactylus, Gyrodactyloides and Laminiscus.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e47"},"PeriodicalIF":1.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aestivation and its impact on the survival of snail intermediate hosts and trematode transmission in rice paddies. 稻田消暑对蜗牛中间寄主存活及吸虫传播的影响。

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-03-10 DOI: 10.1017/S0022149X25000252

K Chantima, K Suk-Ueng, T Tananoi, T Prasertsin

{"title":"Aestivation and its impact on the survival of snail intermediate hosts and trematode transmission in rice paddies.","authors":"K Chantima, K Suk-Ueng, T Tananoi, T Prasertsin","doi":"10.1017/S0022149X25000252","DOIUrl":"10.1017/S0022149X25000252","url":null,"abstract":"In this study, we investigated the diversity and survival of aestivating snails in dry-season rice paddies, focusing on their role as intermediate hosts for trematodes. A total of 1,159 snails from various families were collected and analysed, revealing nine species, primarily from the Bithyniidae family, with Bithynia siamensis siamensis being the most abundant. Of the nine species collected, the most common species, B. s. siamensis, exhibited a relatively high survival rate of 81.5% throughout the three-month dry period post-harvest, whereas the remaining eight species had survival rates below 24.0% (0-23.1%). Four snail species, B. s. siamensis, Filopaludina martensi martensi, F. sumatrensis polygramma, and F. doliaris, were found infected with larval trematodes, with an overall prevalence of 8.46% (57/674). The larval trematodes identified were categorized into four types: echinostome metacercaria, Thapariella anastomusa metacercaria, unidentified metacercaria, and unidentified rediae. These findings demonstrate that trematode infections can persist in aestivating snails despite the challenging conditions during the dry season, emphasizing the potential of aestivating snails to act as vectors for trematode transmission in agricultural settings. This underscores the need for effective management strategies to mitigate the risks associated with trematode transmission.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e43"},"PeriodicalIF":1.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The history continues: From Steringotrema microacetabularis (Fellodistomidae) to Bacciger microacetabularis (Baccigeridae) genetic evidence reveals a new genus of Cryptogonimidae. 历史还在继续：从微髋臼胸甲（Fellodistomidae）到微髋臼芽孢杆菌（Bacciger microacetabularis），遗传证据揭示了隐虫科的一个新属。

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-03-10 DOI: 10.1017/S0022149X25000239

M M Montes, J Barneche, T Acuña Gonzalez, G Cavallo, M Ibañez Shimabukuro, M Moncada, S R Martorelli

{"title":"The history continues: From Steringotrema microacetabularis (Fellodistomidae) to Bacciger microacetabularis (Baccigeridae) genetic evidence reveals a new genus of Cryptogonimidae.","authors":"M M Montes, J Barneche, T Acuña Gonzalez, G Cavallo, M Ibañez Shimabukuro, M Moncada, S R Martorelli","doi":"10.1017/S0022149X25000239","DOIUrl":"10.1017/S0022149X25000239","url":null,"abstract":"Steringotrema microacetabularis Suriano & Martorelli, 1983 (Fellodistomidae) was described from the flounder Paralichthys orbignyanus. Later, it was redescribed, based on new material from the same host and type locality, and reconsidered as Bacciger microacetabularis (Baccigeridae). The main difference noted in the redescription was the presence of spines on the body. However, the lack of DNA data made confirming the true affiliation of this digenean challenging. New specimens sampled from P. orbignyanus allowed us to sequence the 28S, ITS, and COI genes. Fresh specimens were stained to compare their morphology with the holotype and voucher specimens. The digeneans found correspond with those reported from Mar Chiquita, described as B. microacetabularis (=S. microacetabularis). Genetic analyses clustered the newly sequenced individuals within the Cryptogonimidae, showing relationships with Oligogonotylus manteri, Tabascotrema verai, and Caecincola parvulus (28S); T. verai, Lobosorchis spp., Euryakaina manilensis, and Metadena marina (ITS); and Siphoderina spp. (COI). After comparing the species with cryptogonimids lacking spines in the oral sucker, a new genus, Surianotrema n. gen., is described. This genus increases the number of cryptogonimid genera known in South America to seven - three in freshwater and four in marine environments - with Surianotrema n. gen. being the first to be sequenced. New sequences from other South American cryptogonimids are necessary to better understand the phylogenetic relationships between genera within this family, particularly in South America.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e46"},"PeriodicalIF":1.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Twenty thousand fishes under the seas: Insights into the collection and storage of trematodes from the examination of 20,000 fishes in the tropical Indo west-Pacific. 海底的二万条鱼：从对热带印度洋西太平洋的二万条鱼的检查中了解吸虫的收集和储存。

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-03-07 DOI: 10.1017/S0022149X24000968

S C Cutmore, R A Bray, D C Huston, S B Martin, T L Miller, N Q-X Wee, R Q-Y Yong, T H Cribb

{"title":"Twenty thousand fishes under the seas: Insights into the collection and storage of trematodes from the examination of 20,000 fishes in the tropical Indo west-Pacific.","authors":"S C Cutmore, R A Bray, D C Huston, S B Martin, T L Miller, N Q-X Wee, R Q-Y Yong, T H Cribb","doi":"10.1017/S0022149X24000968","DOIUrl":"10.1017/S0022149X24000968","url":null,"abstract":"The techniques employed to collect and store trematodes vary between research groups, and although these differences are sometimes necessitated by distinctions in the hosts examined, they are more commonly an artefact of instruction. As a general rule, we tend to follow what we were taught rather than explore new techniques. A major reason for this is that there are few technique papers in the published literature. Inspired by a collaborative workshop at the Trematodes 2024 symposium, we outline our techniques and processes for collecting adult trematodes from fishes and discuss the improvements we have made over 40 years of dissections of 20,000+ individual marine fishes. We present these techniques for two reasons: first, to encourage unified methods across the globe, with an aim to produce optimally comparable specimens across temporal periods, across geographic localities, and between research groups; and second, as a resource for inexperienced researchers. We stress the importance of understanding differences in host biology and the expected trematode fauna, which ultimately enables organised and productive dissections. We outline our dissection method for each key organ separately, discuss handling, fixation, and storage methods to generate the most uniform and comparable samples, and explore ethical considerations, issues of accurate host identification, and the importance and potential of clear record keeping.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e45"},"PeriodicalIF":1.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0