Journal of Helminthology最新文献

Aestivation and its impact on the survival of snail intermediate hosts and trematode transmission in rice paddies.

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-03-10 DOI: 10.1017/S0022149X25000252

K Chantima, K Suk-Ueng, T Tananoi, T Prasertsin

{"title":"Aestivation and its impact on the survival of snail intermediate hosts and trematode transmission in rice paddies.","authors":"K Chantima, K Suk-Ueng, T Tananoi, T Prasertsin","doi":"10.1017/S0022149X25000252","DOIUrl":"https://doi.org/10.1017/S0022149X25000252","url":null,"abstract":"In this study, we investigated the diversity and survival of aestivating snails in dry-season rice paddies, focusing on their role as intermediate hosts for trematodes. A total of 1,159 snails from various families were collected and analysed, revealing nine species, primarily from the Bithyniidae family, with Bithynia siamensis siamensis being the most abundant. Of the nine species collected, the most common species, B. s. siamensis, exhibited a relatively high survival rate of 81.5% throughout the three-month dry period post-harvest, whereas the remaining eight species had survival rates below 24.0% (0-23.1%). Four snail species, B. s. siamensis, Filopaludina martensi martensi, F. sumatrensis polygramma, and F. doliaris, were found infected with larval trematodes, with an overall prevalence of 8.46% (57/674). The larval trematodes identified were categorized into four types: echinostome metacercaria, Thapariella anastomusa metacercaria, unidentified metacercaria, and unidentified rediae. These findings demonstrate that trematode infections can persist in aestivating snails despite the challenging conditions during the dry season, emphasizing the potential of aestivating snails to act as vectors for trematode transmission in agricultural settings. This underscores the need for effective management strategies to mitigate the risks associated with trematode transmission.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e43"},"PeriodicalIF":1.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The history continues: From Steringotrema microacetabularis (Fellodistomidae) to Bacciger microacetabularis (Baccigeridae) genetic evidence reveals a new genus of Cryptogonimidae.

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-03-10 DOI: 10.1017/S0022149X25000239

M M Montes, J Barneche, T Acuña Gonzalez, G Cavallo, M Ibañez Shimabukuro, M Moncada, S R Martorelli

{"title":"The history continues: From Steringotrema microacetabularis (Fellodistomidae) to Bacciger microacetabularis (Baccigeridae) genetic evidence reveals a new genus of Cryptogonimidae.","authors":"M M Montes, J Barneche, T Acuña Gonzalez, G Cavallo, M Ibañez Shimabukuro, M Moncada, S R Martorelli","doi":"10.1017/S0022149X25000239","DOIUrl":"https://doi.org/10.1017/S0022149X25000239","url":null,"abstract":"Steringotrema microacetabularis Suriano & Martorelli, 1983 (Fellodistomidae) was described from the flounder Paralichthys orbignyanus. Later, it was redescribed, based on new material from the same host and type locality, and reconsidered as Bacciger microacetabularis (Baccigeridae). The main difference noted in the redescription was the presence of spines on the body. However, the lack of DNA data made confirming the true affiliation of this digenean challenging. New specimens sampled from P. orbignyanus allowed us to sequence the 28S, ITS, and COI genes. Fresh specimens were stained to compare their morphology with the holotype and voucher specimens. The digeneans found correspond with those reported from Mar Chiquita, described as B. microacetabularis (=S. microacetabularis). Genetic analyses clustered the newly sequenced individuals within the Cryptogonimidae, showing relationships with Oligogonotylus manteri, Tabascotrema verai, and Caecincola parvulus (28S); T. verai, Lobosorchis spp., Euryakaina manilensis, and Metadena marina (ITS); and Siphoderina spp. (COI). After comparing the species with cryptogonimids lacking spines in the oral sucker, a new genus, Surianotrema n. gen., is described. This genus increases the number of cryptogonimid genera known in South America to seven - three in freshwater and four in marine environments - with Surianotrema n. gen. being the first to be sequenced. New sequences from other South American cryptogonimids are necessary to better understand the phylogenetic relationships between genera within this family, particularly in South America.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e46"},"PeriodicalIF":1.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Twenty thousand fishes under the seas: Insights into the collection and storage of trematodes from the examination of 20,000 fishes in the tropical Indo west-Pacific.

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-03-07 DOI: 10.1017/S0022149X24000968

S C Cutmore, R A Bray, D C Huston, S B Martin, T L Miller, N Q-X Wee, R Q-Y Yong, T H Cribb

{"title":"Twenty thousand fishes under the seas: Insights into the collection and storage of trematodes from the examination of 20,000 fishes in the tropical Indo west-Pacific.","authors":"S C Cutmore, R A Bray, D C Huston, S B Martin, T L Miller, N Q-X Wee, R Q-Y Yong, T H Cribb","doi":"10.1017/S0022149X24000968","DOIUrl":"https://doi.org/10.1017/S0022149X24000968","url":null,"abstract":"The techniques employed to collect and store trematodes vary between research groups, and although these differences are sometimes necessitated by distinctions in the hosts examined, they are more commonly an artefact of instruction. As a general rule, we tend to follow what we were taught rather than explore new techniques. A major reason for this is that there are few technique papers in the published literature. Inspired by a collaborative workshop at the Trematodes 2024 symposium, we outline our techniques and processes for collecting adult trematodes from fishes and discuss the improvements we have made over 40 years of dissections of 20,000+ individual marine fishes. We present these techniques for two reasons: first, to encourage unified methods across the globe, with an aim to produce optimally comparable specimens across temporal periods, across geographic localities, and between research groups; and second, as a resource for inexperienced researchers. We stress the importance of understanding differences in host biology and the expected trematode fauna, which ultimately enables organised and productive dissections. We outline our dissection method for each key organ separately, discuss handling, fixation, and storage methods to generate the most uniform and comparable samples, and explore ethical considerations, issues of accurate host identification, and the importance and potential of clear record keeping.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e45"},"PeriodicalIF":1.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supplemental description of Stephanoprora ornata Odhner, 1902 (Digenea: Echinochasmidae) infecting the Nile crocodile, Crocodylus niloticus (Crocodylidae) from Namibia with emendation of Mesorchis Dietz, 1909 and a phylogenetic analysis - CORRIGENDUM.

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-03-07 DOI: 10.1017/S0022149X25000240

K Cajiao-Mora, H R Dutton, F J Jacobs, P C Beytell, E C Netherlands, L H DuPreez, S A Bullard

引用次数: 0

Genetic identification of Stephanofilaria sp. isolated from ulcerative dermal lesions in black rhinoceros.

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-03-06 DOI: 10.1017/S0022149X25000112

P I Chiyo, E King'ori

{"title":"Genetic identification of Stephanofilaria sp. isolated from ulcerative dermal lesions in black rhinoceros.","authors":"P I Chiyo, E King'ori","doi":"10.1017/S0022149X25000112","DOIUrl":"https://doi.org/10.1017/S0022149X25000112","url":null,"abstract":"Stephanofilaria is a genus of nematodes that cause ulcerative dermal lesions in large mammals. However, there is a dearth of knowledge on the molecular genetics of Stephanofilaria species infecting critically endangered rhinoceros. This study employed genetic barcoding genes to identify Stephanofilaria species and to determine its genetic diversity and evolution. Phylogenetic analyses on partial genes of the second internal transcribed spacer Ribosomal DNA (ITS-2) and cytochrome c oxidase subunit 1 (Cox-1), revealed a 77% and 93% bootstrap support at the Cox-1 and ITS-2 loci respectively to a clade containing previously identified Stephanofilaria species. Morphological examination also confirmed features diagnostic of Stephanofilaria dinniki previously known to infect rhinoceros. Gene diversity of Cox-1 was 0.931 ± 0.030 and 0.579 ± 0.104 for the ITS-2, whereas nucleotide diversity was 0.008 ± 0.002 and 0.00197 ± 0.0016 for the Cox-1 and ITS-2 genes respectively. Neutrality tests (Fu and Li's D* and Fu and Li's F*) were significantly negative (p<0.05) at all loci, whereas Tajima D and Fu's FS were each statistically significant (p<0.05) at the Cox-1 and ITS-2 loci respectively. The high gene diversity, low nucleotide diversity and negative neutrality tests are consistent with positive selection at the Cox-1 gene. Stephanofilaria infection among rhinoceros is currently restricted to highland sanctuaries compared to a widespread distribution in both lowlands and highlands in the 1960s suggesting an adaptation to vectors thriving in cooler highland temperatures. This is the first genetic identification of S. dinniki, in rhinoceros and will aid in diagnosis, treatment, studies, and rhinoceros conservation.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e42"},"PeriodicalIF":1.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ParasiteBlitz: Adaptation of the BioBlitz concept to parasitology.

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-03-06 DOI: 10.1017/S0022149X25000197

I de Buron, K M Hill-Spanik, S D Atkinson, M P M Vanhove, N Kmentová, S Georgieva, D M Díaz-Morales, M R Kendrick, W A Roumillat, G K Rothman

{"title":"ParasiteBlitz: Adaptation of the BioBlitz concept to parasitology.","authors":"I de Buron, K M Hill-Spanik, S D Atkinson, M P M Vanhove, N Kmentová, S Georgieva, D M Díaz-Morales, M R Kendrick, W A Roumillat, G K Rothman","doi":"10.1017/S0022149X25000197","DOIUrl":"https://doi.org/10.1017/S0022149X25000197","url":null,"abstract":"A BioBlitz is a rapid and intensive survey of a specific geographic area that brings together experts and often lay participants to assess biodiversity, typically of macrobiota that are easily observed and identifiable on-site. This concept has become popular across taxonomic fields, attracting interest globally to increase knowledge of local biodiversity. Inspired by the success of the approach, we undertook a 'ParasiteBlitz' at an unexplored locality (Stono Preserve, Charleston, South Carolina, USA) to determine its feasibility for parasites, whose assessment of diversity is largely neglected worldwide. We assembled a team of parasitologists with complementary expertise. Over 12 days (3 days in each habitat) in April 2023, we intensively screened fishes and aquatic invertebrates for parasites, and sampled sediment and water for environmental DNA (eDNA) metabarcoding from four aquatic habitats: wetland, freshwater pond, brackish impoundment, and tidal creek. We incorporated assistance from non-parasitologists and students. Details on methodologies and results are provided in individual papers in this Special Collection. Traditional methods revealed the presence of ca. 100 species of seven major metazoan parasite taxa, and the eDNA survey yielded over 1,000 amplicon sequence variants identified as parasites, most with sequences unmatched in GenBank, and resulting in only a few species identified as named species in the one-year post-Blitz timeframe we imposed upon ourselves for identification. Limitations and challenges of the ParasiteBlitz are discussed, and our results support that this approach can be effective for rapid discovery of the dimensions of parasite assemblages in an understudied environment and contribute to parasitology knowledge.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e39"},"PeriodicalIF":1.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and prevalence assessment of Durettenema sp. (Nematoda: Trichostrongyloidea) in the great leaf-nosed bats (Hipposideros armiger) in South China.

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-03-06 DOI: 10.1017/S0022149X25000227

Y Hu, Y Gan, Y Chen, H Wang, X Cui, Y Shen, X Shen

{"title":"Molecular characterization and prevalence assessment of Durettenema sp. (Nematoda: Trichostrongyloidea) in the great leaf-nosed bats (Hipposideros armiger) in South China.","authors":"Y Hu, Y Gan, Y Chen, H Wang, X Cui, Y Shen, X Shen","doi":"10.1017/S0022149X25000227","DOIUrl":"https://doi.org/10.1017/S0022149X25000227","url":null,"abstract":"The viruses associated with bats have generated significant concern; however, there is limited knowledge regarding the endoparasites that affect these mammals. This study involved the collection of seven nematode specimens (three males and four females) from the intestines of Hipposideros armiger in Shaoguan City, Guangdong, China. Next-generation sequencing was employed to obtain the mitochondrial DNA (mtDNA) genome, which was determined to be 14,130 base pairs in length. The mitochondrial genome comprised 12 protein-coding genes, 21 tRNA genes, 2 rRNA genes, and an AT-rich non-coding region. Phylogenetic analyses based on mtDNA sequences indicated that the nematode forms a sister clade to Nematodirus, exhibiting only 74% nucleotide identity. In contrast, the nuclear ITS1 gene demonstrated a high degree of nucleotide identity (98.6%-98.8%) with Durettenema guangdongense. Consequently, the parasitic nematode identified from H. armiger is likely to belong to the genus Durettenema and has been designated as Durettenema sp. 888. Furthermore, an epidemiological investigation revealed the presence of the parasitic nematode infections in H. armiger collected from Guangdong, Guangxi, and Guizhou Provinces. Given the widespread distribution of H. armiger and their tendency to inhabit areas in close proximity to human dwellings, the influence of parasite prevalence on bat population numbers and potential for human and domestic animal transmission of this pathogen warrants further investigation.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e41"},"PeriodicalIF":1.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The association between Parkinson disease and Toxocara infection/exposure: A case-control study.

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-03-05 DOI: 10.1017/S0022149X25000161

A Alizadeh Khatir, A Abbasi, S Sarandili, M Sepidarkish, A Fazlollahpour-Naghibi, D Arjmandi, A Rostami

{"title":"The association between Parkinson disease and Toxocara infection/exposure: A case-control study.","authors":"A Alizadeh Khatir, A Abbasi, S Sarandili, M Sepidarkish, A Fazlollahpour-Naghibi, D Arjmandi, A Rostami","doi":"10.1017/S0022149X25000161","DOIUrl":"https://doi.org/10.1017/S0022149X25000161","url":null,"abstract":"Parkinson's disease (PD) is a prevalent neurological disorder and the second most common neurodegenerative disease. Research has explored the impact of infectious agents, such as the parasites, on neurological conditions, including PD. Given the limited studies worldwide and in Iran, this study aims to investigate the relationship between Toxocara infection and PD. This case-control study involved 91 PD patients and 90 healthy controls. After obtaining consent, serum samples and questionnaires were collected. All sera were examined using an ELISA test for IgG antibodies against Toxocara canis. Results were analyzed with SPSS, using chi-square tests, and odds ratios (OR), and confidence intervals (CI) were calculated via univariate and multivariate analyses. The prevalence of anti-Toxocara IgG was 33% (30/91) in PD patients and 33.3% (30/90) in the control group. Both univariate analysis (OR: 0.98; 95% CI: 0.52-1.82) and multivariate analysis (OR: 0.95; 95% CI: 0.49-1.83) indicated no statistically significant association. Additionally, univariate analysis (OR: 0.49; 95% CI: 0.16-1.5) and multivariate analysis (OR: 0.37; 95% CI: 0.09-1.43) suggested non-significant association between Toxocara infection and the severity of PD. Our findings do not support a statistically significant association between Toxocara infection and the PD. While the analysis suggested that Toxocara infection might reduce the severity of PD, these results were also not statistically significant. Further research with larger sample sizes and diverse populations is needed to fully understand the potential relationship between Toxocara infection and PD.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e40"},"PeriodicalIF":1.3,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of Oscheius insectivorus (Körner, 1954) Andrássy, 1976 (Nematoda: Rhabditidae) from Iran with its efficacy against Chrysomela populi L. (Col.: Chrysomelidae) and Galleria mellonella L. (Lep.: Pyralidae).

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-02-28 DOI: 10.1017/S0022149X25000070

M Amiri Bonab, M R Atighi, R Gholami Ghavamabad, M Pedram

{"title":"First report of Oscheius insectivorus (Körner, 1954) Andrássy, 1976 (Nematoda: Rhabditidae) from Iran with its efficacy against Chrysomela populi L. (Col.: Chrysomelidae) and Galleria mellonella L. (Lep.: Pyralidae).","authors":"M Amiri Bonab, M R Atighi, R Gholami Ghavamabad, M Pedram","doi":"10.1017/S0022149X25000070","DOIUrl":"https://doi.org/10.1017/S0022149X25000070","url":null,"abstract":"A population of Oscheius insectivorus was recovered from inside the body of a live scarab beetle collected from natural forests of Jafar Abad village, Golestan province, and was reported from Iran for the first time. The Iranian isolate of the species is characterized by 1103-1942 μm long females with their vulva at 46.9-57.4% of the body, tail 75-118 μm long, males with spicules 60.0-74.5 μm long, gubernaculum 23.7-32.2 μm long, and infective juveniles measuring 790-985 μm long. Females and males of the recovered population had shorter body when compared with the type population. Molecular identification of the recovered population was performed using the SSU and D2-D3 expansion segments of LSU rDNA, and corresponding phylogenetic trees were reconstructed and discussed. The pathogenicity of the Iranian isolate was evaluated on the larvae and adults of the poplar leaf beetle, Chrysomela populi, and the larvae of the wax moth, Galleria mellonella, under laboratory conditions. A suspension of 200 infective juveniles per ml (IJs/ml) of the nematode was most effective against third instar larvae of C. populi, causing 75% mortality 72 hours post-exposure. In adults, mortality reached 70% at the same concentration and exposure time. For G. mellonella larvae, O. insectivorus caused 50% mortality at 200 IJs/ml within 72 hours post-exposure. The lethal concentration 50 (LC50) values of the nematode were 77.71 and 94.06 IJs/ml for third instar larvae and adults of C. populi, respectively, and 217.04 IJs/ml for fifth instar larvae of G. mellonella 72 hours post-exposure at 25°C and 60% relative humidity.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e36"},"PeriodicalIF":1.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Life cycle of the seabird digenean Gymnophallus minor (Gymnophallidae) in the Arctic.

IF 1.3 3区生物学

Journal of Helminthology Pub Date : 2025-02-27 DOI: 10.1017/S0022149X25000173

K V Galaktionov, A Gonchar, K M Wegner, R Wolfensberger, C Buschbaum, A E Romanovich

引用次数: 0