{"title":"Population dynamics of a South African isolate of <i>Steinernema yirgalemense in vitro</i> liquid culture, using egg yolk as protein source.","authors":"M D Dunn, A P Malan","doi":"10.1017/S0022149X24000452","DOIUrl":"https://doi.org/10.1017/S0022149X24000452","url":null,"abstract":"<p><p>The entomopathogenic nematode (EPN), <i>Steinernema yirgalemense</i>, is an effective biological control agent against a variety of important insect pests in South Africa. To develop a South African EPN product feasibly in South Africa, EPNs need to be mass-produced. This study aimed to record the population dynamics of <i>S. yirgalemense</i> with <i>in vitro</i> liquid production in shake flasks, with a protein source of powdered egg yolk. The Erlenmeyer flask results indicated variation between flasks, albeit still achieving high yields. The reasons for attaining such variability in the recovery, yield, and growth stages are unclear, hence requiring further studies seeking to increase consistency. The results obtained indicate that, when IJ recovery is low, yields are also low due to relatively few reproductive adults being present in solution, which, in turn, produces more offspring, which later converts to the desired infective juvenile used for product formulation development. For commercial viability, a consistent production system is required that produces predictable yields. This study showed comparable high yields achieved with the flasks and in an early-stage bioreactor setup, being a positive development for <i>S. yirgalemense</i> mass production. Prior to the bioreactor scale-up process, protocol of mass production, the population and growth dynamics of the nematodes in the flask environment requires understanding. This is a positive step, leading to the future commercialisation of a local EPN product.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y Velázquez-Urrieta, M García-Varela, G Pérez-Ponce de León
{"title":"Assessing the diversity of freshwater fish trematodes from Laguna Escondida, Los Tuxtlas tropical rainforest, Mexico, using morphology and 28S rDNA sequences as barcodes.","authors":"Y Velázquez-Urrieta, M García-Varela, G Pérez-Ponce de León","doi":"10.1017/S0022149X2400049X","DOIUrl":"https://doi.org/10.1017/S0022149X2400049X","url":null,"abstract":"<p><p>Despite a great effort made for almost 90 years, the diversity of freshwater fish trematodes in Mexico is still far from being fully known. The addition of molecular data to the description of trematode diversity in the last two decades added the potential to establish more robust species limits and a more accurate biodiversity estimation, but also led in some instances to the recognition of cryptic species complexes. Here, we used sequences of the large subunit of the nuclear ribosomal gene (28S rRNA) as barcodes, and morphological data, to assess the diversity of freshwater fish trematodes from a lake within a tropical rainforest. Eighty freshwater fish specimens of eight species were studied, and 120 trematode specimens were collected. Morphologically, specimens were allocated into nine genera; molecular phylogenetic analyses along with sequence divergence data provided evidence for recognising 11 trematode taxa, six adults and five metacercariae; six of them were identified to species level. Geographical distribution and host association patterns are briefly discussed for each trematode taxa.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R Kaveh, N Ashrafhesari, S M Mousavi, M Fasihi Harandi
{"title":"Narrowing gender gap in the research on echinococcosis and editorial contribution of women in parasitology journals.","authors":"R Kaveh, N Ashrafhesari, S M Mousavi, M Fasihi Harandi","doi":"10.1017/S0022149X24000579","DOIUrl":"https://doi.org/10.1017/S0022149X24000579","url":null,"abstract":"<p><p>Gender equity and authorship diversity are believed to be the essential parts of building a dynamic scientific atmosphere. The purpose of the present study was to determine the status of gender equity in research on echinococcosis and the editorial diversity in major parasitology journals over the past four decades. All articles were retrieved from major databases from the years 1980, 2000, 2010, 2015, and 2020. Journals belonging to the four quartiles of parasitology journals listed in the Journal Citation Report were selected, and the gender and region of each editorial member were identified. Among the 3583 first authors of the articles published in all selected years, 2236 (62.4%) were men, whereas 1040 (29%) were women. There was a significant increase in women's contributions as the first author, from 6.8% in 1980 to 35.8% in 2020 (<i>P</i> < .001). A greater gender gap was found for the senior authors, showing 2391 (66.7%) men and 837 (23.4%) women. The gender gap has been narrowed in most of the six regions of the world, particularly for the Western Pacific region, where the gender inequity had almost diminished in 2020; i.e. the man-woman ratios of the first and last authors from this region were 2.25 and 1.75 in 1980, reaching 1.04 and 0.97, in 2020, respectively. Our findings also indicated that articles authored by men received 2.5 to 3.1 times more citations than women authors. Gender distribution of the editors-in-chief, associate editors, and editorial board members across all quartiles showed that 78.7%, 69.5%, and 72.7% were men, respectively, and mostly affiliated with the European and American regions. Findings of the present study showed that gender inequity is still present and women researchers continue to be the minority in the field of parasitology, particularly in the research on echinococcosis.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"New record and phylogenetic assessment of <i>Apopharynx bolodes</i> (Braun, 1902) (Digenea: Psilostomidae), a parasite of Eurasian Coot <i>Fulica atra</i> Linnaeus, 1758 (Aves: Rallidae).","authors":"S A Vlasenkov, L N Akimova, S G Sokolov","doi":"10.1017/S0022149X24000543","DOIUrl":"https://doi.org/10.1017/S0022149X24000543","url":null,"abstract":"<p><p>Phylogenetic studies of aberrant species are of considerable scientific interest because their taxonomic rank in traditional systems based on morphological characters is not infrequently overestimated. <i>Apopharynx bolodes</i> (Braun, ) is one of the few psilostomid digeneans devoid of the pharynx. This is considered a sufficient basis for assigning it and similar species to the subfamily Apopharynginae. We found <i>A. bolodes</i> in <i>Fulica atra</i> Linnaeus, 1758 from Belarus, described it morphologically, and genotyped it by the 28S rRNA gene and the ITS2 region. It is the first molecular data on <i>A. bolodes</i> and the first record of this digenean species in Belarus. The phylogenetic analysis based on partial sequences of the 28S rRNA gene showed that <i>A. bolodes</i> is closely related to the <i>Sphaeridiotrema</i> spp. (Sphaeridiotrematinae). However, this phylogenetic inference has not received yet support with data on the ITS2 region.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N G Ruiz-Torres, D I Hernandez-Mena, L García-Prieto, L I Falcón, J A Panti-May, O Rico-Chávez
{"title":"A new genus and species of Microphalloidea (Digenea), parasite of <i>Peropteryx</i> spp. (Chiroptera: Emballonuridae) from the Neotropical region of Mexico revealed by morphological and phylogenetic analyses.","authors":"N G Ruiz-Torres, D I Hernandez-Mena, L García-Prieto, L I Falcón, J A Panti-May, O Rico-Chávez","doi":"10.1017/S0022149X24000531","DOIUrl":"10.1017/S0022149X24000531","url":null,"abstract":"<p><p>The present study aims to describe a new genus and species of microphalloid digenean parasite of two species of bats of the genus <i>Peropteryx</i> from the Mexican Neotropics (in the states of Chiapas and Yucatán). Morphological and molecular data (28s rDNA ribosomal gene sequences) were used to study Digeneans. <i>Sagittatrema zutzi</i> gen. nov. sp., nov., is diagnosed morphologically by having a sagittiform body, a genital pore in the midline of the body, posterior to the ventral sucker, and a cirrus sac running through much of the diameter of the ventral sucker. The nine sequences generated from the 28S rDNA gene were used to examine the phylogenetic affinities of this new taxon within the superfamily Microphalloidea Ward, 1901, using Maximum Likelihood and Bayesian Inference analyses. Both analyses resulted in trees with similar topologies and formed a well-supported clade (Bt = 100; pp = 1) with the <i>Sagittatrema</i> sequences. Because of the new genus's phylogenetic position and that some sister families to the proposed taxa, like Pleurogenidae and Prosthogonimidae, are polyphyletic, we prefer to consider <i>Sagittatrema</i> as a genus <i>incertae sedis</i> within Microphalloidea. A full systematic review of microphalloids is needed to confirm their phylogenetic position.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P S Dos Santos, I C O Silva, V L Ferreira, L E R Tavares, F Paiva, F B Pereira
{"title":"First genetic characterisation and phylogenetic position of <i>Aplectana hylambatis</i> (Nematoda: Cosmocercidae), infecting <i>Pithecopus azureus</i> (Anura: Hylidae) in the Brazilian Pantanal.","authors":"P S Dos Santos, I C O Silva, V L Ferreira, L E R Tavares, F Paiva, F B Pereira","doi":"10.1017/S0022149X24000609","DOIUrl":"10.1017/S0022149X24000609","url":null,"abstract":"<p><p><i>Aplectana hylambatis</i> (Baylis, 1927) is a widespread nematode in South America that infects different species of anurans from different families. Although the morphology of this species shows intraspecific variations and is well studied, <i>A. hylambatis</i> does not have genetic characterisation. Therefore, the present study aimed to provide the first genetic characterisation of this parasite, based on 18S and 28S rDNA sequences, as well as to evaluate its phylogenetic position in the family Cosmocercidae. The present specimens showed no major morphological variations and were assigned to <i>A. hylambatis</i> mainly based on the shape of spicules and the presence of mamelon-like structures slightly anterior to the vulva. The genetic characterisation confirmed the close relationship between <i>A. hylambatis</i> and other cosmocercids, and the 28S sequences were more informative for phylogenetic reconstruction, especially for those relationships at higher and intermediate levels. <i>Aplectana hylmabatis</i> was in a basal position within a clade formed by other congeners and species of <i>Cosmocerca.</i> The geographic origin may have influence in the divergence process of <i>Aplectana</i> spp. and genus was non-monophyletic and closely related to <i>Cosmocerca</i>, similar to previous findings. The present results will be useful for future studies on the phylogeny of Cosmocercidae, since several genera and species in this family still lack genetic characterisation. Furthermore, the 28S sequences can be useful in genetic studies of <i>A. hylambatis</i> populations, since this species appears to have phenotypic variations.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Is <i>Schistosoma mansoni</i> playing a part in liver carcinogenesis?","authors":"S Jain","doi":"10.1017/S0022149X24000506","DOIUrl":"https://doi.org/10.1017/S0022149X24000506","url":null,"abstract":"<p><p>The relationship between <i>Schistosoma mansoni</i> (Sm) and hepatocellular carcinoma (HCC) has been evaluated by many studies that point towards a co-relation between schistosomal infection and HCC. While many such studies demonstrated that Sm infection in the presence of another carcinogenic factors leads to HCC, none of these studies could conclusively prove the cancer-inducing ability of Sm in humans, independent of other carcinogenic factors. The aim of this work is to present the current understanding on the association of Sm with HCC. Many epidemiological, pathological, and clinical studies have shown the role of multiple events like chronic inflammation and fibrosis as well as hepato-toxic agents like soluble egg antigens (SEAs), which help in creating a micro-environment which is suitable for HCC development. The role of Sm infection and deposited eggs in causing persistent inflammation, advanced fibrosis, and the role of SEAs, especially IPSE/alpha-1, is emphasised. This work concludes that Sm infection has the potential to induce cancer independently but the same has not been reported in humans to date. Extensive research is required to establish a causal relationship between Sm infection and HCC induction, or a complete lack thereof. However, Sm infection definitely acts along with other carcinogenic factors to induce HCC at a much faster pace and also leads to an aggressive form of liver cancer, which the other carcinogenic factor could not have achieved alone.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142522060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Never ending diversity: two new species of the genus <i>Allocreadium</i> (Digenea: Allocreadiidae) including new keys to the genus.","authors":"K S Vainutis","doi":"10.1017/S0022149X24000440","DOIUrl":"https://doi.org/10.1017/S0022149X24000440","url":null,"abstract":"<p><p>Two new species of the genus <i>Allocreadium</i> were isolated from the intestines of the Lake minnow <i>Rhynchocypris percnura</i> caught in the backwater of the Komissarovka River in the South of the Russian Far East. The morphology of <i>A. anastasii</i> n. sp. corresponds to that of <i>Allocreadium</i> sp. from Lake Khar (Mongolia) and <i>Allocreadium</i> sp. Belous, 1952 from the Primorsky region of Russia except for the preacetabular anterior border of the vitelline follicles in <i>A. anastasii</i> n. sp. from the Komissarovka River vs. at anterior half of ventral sucker in <i>Allocreadium</i> sp. Genetic analysis revealed the identity of <i>A. anastasii</i> n. sp. to <i>Allocreadium</i> sp. 1 from the Nezhinka River and Lake Khar. <i>Allocreadium macrolecithum</i> n. sp. was differentiated from Palaearctic <i>Allocreadium</i> spp. by having the following features: respectively large vitelline follicles extending from posterior extremity to anterior margin of the ventral sucker; relatively short caeca reaching the border of middle and posterior thirds of hindbody; and small testes in the middle of hindbody. Interspecific genetic p-distances between <i>Allocreadium</i> spp. were 0.16-7.23% in 28S gene and 18.62-31.54% in <i>Cox1</i> mtDNA gene. In the phylogenetic tree reconstructed with Maximum parsimony and Bayesian Inference methods, <i>A. anastasii</i> n. sp. and <i>A. macrolecithum</i> n. sp. were nested into different species groups of the genus <i>Allocreadium</i> - sister to <i>A. khankaiense</i> and <i>A. bursense</i>, respectively. Modified dichotomous keys were prepared for 31 Palaearctic species of <i>Allocreadium</i> including <i>A. crassum</i>, <i>A. dogieli</i>, <i>A. papilligerum</i>, <i>A. bursense</i>, <i>A. anastasii</i> n. sp., and <i>A. macrolecithum</i> n. sp.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y Aldama-Prieto, M T González-García, B Mendoza-Garfías, G Pérez-Ponce de León, M García-Varela
{"title":"Integrative taxonomy reveals two new species of <i>Maritrema</i> Nicoll, 1907 (Digenea: Microphallidae Ward, 1901), parasitizing birds in the Gulf of Mexico.","authors":"Y Aldama-Prieto, M T González-García, B Mendoza-Garfías, G Pérez-Ponce de León, M García-Varela","doi":"10.1017/S0022149X24000403","DOIUrl":"https://doi.org/10.1017/S0022149X24000403","url":null,"abstract":"<p><p>Members of the genus <i>Maritrema</i> Nicoll, 1907 include endoparasites of aquatic birds and mammals, distributed worldwide. Adult specimens were collected from the intestines of three bird species (the great black hawks, <i>Buteogallus urubitinga</i> Gmelin; laughing gull, <i>Leucophaeus atricilla</i> Linnaeus; and the willet, <i>Tringa semipalmata</i> Gmelin) in three localities along the Gulf of Mexico. <i>Photogenophores</i> were sequenced for the large subunit (LSU) from nuclear rDNA, and the new sequences were aligned with other microphalloid sequences available in GenBank. The maximum likelihood and Bayesian inference analyses revealed three independent lineages, one corresponding to a previously described species, <i>Maritrema corai</i> Hernández-Orts, Pinacho-Pinacho, García-Varela & Kostadinova, 2016, and two representing two undescribed species. These two new species are described in the current study. <i>Maritrema itzamnai</i> n. sp. can be morphologically differentiated from its four congeneric species occurring in coastal areas of Mexico by having smaller oral (20-29 × 20-38 μm) and ventral (20-39 × 19-33 μm) suckers. In addition, <i>Maritrema itzamnai</i> n. sp. possess annular vitellarium instead of a horseshoe-shaped vitellarium. <i>Maritrema kukulkanni</i> n. sp. can be distinguished from its congeneric species reported from Mexico by possessing a larger oesophagus (44-117μm) and by having a vitellarium distributed in both the hindbody and forebody. <i>Maritrema corai</i> is the third species recorded in this study parasitizing the great black hawk (<i>Buteogallus urubitinga</i>); this represents a new host and locality record, expanding its distribution range from the Mexican Pacific Ocean to the Gulf of Mexico.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphological and molecular characterisation of two new species of <i>Rhipidocotyle</i> (Digenea: Bucephalidae Poche, 1907) from <i>Sphyraena putnamae</i> Jordan & Seale in Mozambique.","authors":"J C Dumbo, Q M Dos Santos, A Avenant-Oldewage","doi":"10.1017/S0022149X24000476","DOIUrl":"https://doi.org/10.1017/S0022149X24000476","url":null,"abstract":"<p><p>Species-level delineation of digenetic trematodes is complex and can be best achieved by integrative taxonomy using both genetic characterisation and morphological analysis. Two new Bucephalidae species of the genus <i>Rhipidocotyle</i> Diesing, 1858 are described here based on specimens collected from the intestine of <i>Sphyraena putnamae</i> Jordan & Seale following this approach. Adults of <i>R. siphonyaka</i> n. sp. and <i>R. nolwe</i> n. sp. possess tentacles and a tegument with scales. They are distinguished from their congeners by the arrangement of the digestive structures, the extent of the uterus relative to vitelline fields, and the arrangement of the reproductive structures. <i>Rhipidocotyle siphonyaka</i> n. sp. differs from <i>R. nolwe</i> n. sp. in having the pharynx and mouth positioned in the pre-uterine field, tandem testes, longer body length, and shorter pre-vitelline and post-testicular distance. <i>Rhipidocotyle siphonyaka</i> n. sp. differs from its congeners in having a tube-like intestinal caecum, pharynx and mouth opening positioned in the pre-vitelline field. <i>Rhipidocotyle nolwe</i> n. sp. appears to be similar, morphologically and morphometrically, to <i>Rhipidocotyle khalili</i> (Nagaty, 1937). Despite their similarities, <i>R. nolwe</i> n. sp. has a shorter body length and egg size. Moreover, the molecular analysis of 28S and ITS rDNA fragments indicate that <i>R. siphonyaka</i> n. sp. and <i>R. nolwe</i> n. sp. are closely related phylogenetically but distinct from one another and other Bucephalidae for which molecular data are available.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}