Journal of Helminthology最新文献

{"title":"Cercarial productivity of redial generations in single-miracidium infections of Lymnaea truncatula with Paramphistomum daubneyi or Fasciola hepatica","authors":"M. Abrous, D. Rondelaud, G. Dreyfuss","doi":"10.1017/s0022149x00700010","DOIUrl":"https://doi.org/10.1017/s0022149x00700010","url":null,"abstract":"Single-miracidium infections of <jats:italic>Lymnaea truncatula</jats:italic> with <jats:italic>Paramphistomum daubneyi</jats:italic> or with <jats:italic>Fasciola hepatica</jats:italic> were carried out under laboratory conditions to count free rediae, their germinal embryos, and to determine the cercarial productivity of each redial generation. In snails infected by <jats:italic>P. daubneyi</jats:italic>, the cercariae were produced by the first (8.7 cercariae per redia) and second (8.9 per redia) generations. At day 63 post-exposure, they corresponded, respectively, to 53.9% and 46.1% of cercariae produced by all rediae. In snails infected by <jats:italic>F. hepatica</jats:italic>, the majority of cercariae were produced by the R2a group (18.2 cercariae per redia) and corresponded to 66.0% of cercariae produced all rediae. The cercariae produced by the other redial groups were more limited in number: 17.5 per redia in the R1b group (28.7%) and 2.0 per redia in the R2b/R3a group (5.3%). Cercarial productivity of <jats:italic>P. daubneyi</jats:italic> until day 63 post-exposure was more limited in number than that of <jats:italic>F. hepatica</jats:italic>: a total of 145 cercariae per snail versus 427 per snail.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140579384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survey of Spirometra erinaceieuropaei in frogs in Taiwan and its experimental infection in cats","authors":"H.K. Ooi, S.L. Chang, C.C. Huang, Y. Kawakami, A. Uchida","doi":"10.1017/s0022149x00700861","DOIUrl":"https://doi.org/10.1017/s0022149x00700861","url":null,"abstract":"Eighteen of 56 (32.1%) wild <jats:italic>Rana limnocharis</jats:italic> from central and south Taiwan were found to contain plerocercoids of <jats:italic>Spirometra erinaceieuropaei</jats:italic>. This is the first report of <jats:italic>S. erinaceieuropaei</jats:italic> infections in frogs in Taiwan, with the plerocercoids being recovered from the thigh and back muscles or under the skin. Other species of frogs examined, including nine wild <jats:italic>R. latouchii</jats:italic>, one wild <jats:italic>Buergeria robustus</jats:italic> and 110 cultured <jats:italic>R. rugulosa</jats:italic> were free of infection. The plerocercoids were orally inoculated into four cats; three of which were each given a single plerocercoid and one a dose of three plerocercoids. Daily faecal examination showed that two cats started shedding eggs of <jats:italic>S. erinaceieuropaei</jats:italic> on day 8 postinfection (PI) and the other two on day 10 PI. The highest eggs per gram and eggs per day for a single worm was found to be 428,000 and 14,416,000 respectively. Only the cat inoculated with three plerocercoids shed proglottids in its faeces during the 2 month observation period.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140579509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The white grunt, Haemulon plumierii (Lacepède, 1801) as paratenic and definitive host of two acanthocephalan species, with the description of a new species of Dollfusentis (Palaeacanthocephala: Leptohynchoididae) from the Yucatán Peninsula, Mexico.","authors":"M. García-Varela, A. L. Sereno-Uribe, B. Solórzano‐García, G. Pérez‐Ponce de León","doi":"10.1017/S0022149X24000105","DOIUrl":"https://doi.org/10.1017/S0022149X24000105","url":null,"abstract":"Acanthocephalans are a group of obligate endoparasites that alternate between vertebrates and invertebrates to complete their life cycles. Occasionally, the same individual host acts as a definitive or paratenic host for different acanthocephalan species. In this study, acanthocephalans were sampled in marine fish in three localities of the Yucatán Peninsula; adults and cystacanths were recovered from the intestine and body cavity, respectively, of Haemulon plumierii from off the coast of Sisal, Yucatán. Ribosomal DNA sequences (small and large subunits) were used to test the phylogenetic position of the species of the genus Dollfusentis, whereas the mtDNA gene cox 1 was used for assessing species delimitation. The cox 1 analysis revealed an independent genetic lineage, which is recognized herein as a new species, Dollfusentis mayae n. sp. The new species is morphologically distinguished from the other six congeners by having a cylindrical proboscis armed with 22-25 longitudinal rows bearing 12 hooks each. The cystacanths were morphologically identified as Gorgorhynchus medius by having a cylindrical trunk covered with tiny irregular spines on the anterior region, and a cylindrical proboscis armed with 17-18 longitudinal rows of 21 hooks each; small and large subunit phylogenetic analyses yielded G. medius within the family Isthomosacanthidae, suggesting that Gorgorhynchus should be transferred to this family from Rhadinorhynchidae where it is currently allocated.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140729929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Closing the knowledge gap: Helminth parasites of freshwater turtles from the Chaco-Pampa Plain, Southern South America.","authors":"E. Palumbo, Leandro Alcalde, Marcelo Bonino, Julián Lescano, Martín Montes, Agustín Solari, Julia Inés Diaz","doi":"10.1017/S0022149X24000178","DOIUrl":"https://doi.org/10.1017/S0022149X24000178","url":null,"abstract":"Six species of freshwater turtles dominate the Chaco-Pampa Plain in southern South America and their parasites have been relatively understudied, with most records concentrated in Brazil. Particularly in Argentina, there are only scattered records of parasites for most of the turtles that inhabit the region, leaving a large knowledge gap. The purpose of the present contribution is to increase the knowledge of the internal parasites of six species of freshwater turtles from Argentina, after 15 years of fieldwork, by providing new hosts and additional geographic records for many host-parasite relationships. Some molecular sequences of the studied parasites were provided as a tool for better species identification. We processed 433 stomach and fecal samples from live individuals and visceral and soft tissue samples from 54 dissected turtles collected from a wide range and different ecoregions. We found 6230 helminths belonging to 18 taxa (one cestode, 11 digeneans and six nematodes). Fourteen new parasite-host associations are reported here, and for the first time parasites are recorded for Phrynops williamsi. This work contributes significantly to the knowledge of the parasitofauna in freshwater turtles in Argentina, providing a detailed list of parasites present in each turtle species and reporting molecular characters for future studies.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140731935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new species of the genus Hexamermis Steiner, 1924 (Nematoda: Mermithidae) from northern Iran: a nematode with an unusual uterine morphology.","authors":"N. Vazifeh, G. Niknam, N. Camino, F. Abootalebi","doi":"10.1017/S0022149X24000063","DOIUrl":"https://doi.org/10.1017/S0022149X24000063","url":null,"abstract":"Hexamermis zirabi sp. n., recovered from a natural habitat of Mazandaran province, north of Iran, is described based on morphological and molecular data. The new species is characterized by its six cephalic papillae; cuticle with distinct cross fibers; conoid or sharply tapered head; mouth terminal; six hypodermal cords; J-shaped vagina oriented to the anterior end of body; uterus with Z-organs or sclerotized bodies; tail similar in both sexes and bluntly rounded; spicules paired, separate, slightly curved, shorter than body width at cloaca, with rounded tip; and male genital papillae arranged in five rows. In addition to the morphological study, molecular phylogenetic analyses using a partial large subunit (28S D2-D3) were also performed, and the new species formed a highly supported (1.00% Bayesian posterior probability (BPP)) clade with Hexamermis popilliae.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140748129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative taxonomy of <i>Anaporrhutum mundae</i> sp. nov. (Trematoda: Gorgoderidae), a parasite of the Munda round ray <i>Urotrygon munda</i> (Urotrygonidae) in Costa Rica.","authors":"Mario Santoro, Alejandro López-Verdejo, Arturo Angulo, Alicia Rojas, Jorge Cortés, Bernald Pacheco-Chaves, Alberto Solano-Barquero","doi":"10.1017/S0022149X2400018X","DOIUrl":"10.1017/S0022149X2400018X","url":null,"abstract":"<p><p>A new species of trematode of anaporrhutine gorgoderid, from the gill chambers of the Munda round ray <i>Urotrygon munda</i> in Costa Rica is described, based on an integrative taxonomic approach that includes the use of light and scanning electron microscopy, ITS2 and 28S rDNA sequencing, and phylogenetic analysis. <i>Anaporrhutum mundae</i> sp. nov. can be distinguished from congeneric species by a combination of morphological traits and particularly by having the genital pore opening at the level of the intestinal bifurcation. The new species also can be distinguished from all other species of <i>Anaporrhutum</i>, except <i>A. euzeti</i> Curran, Blend & Overstreet, 2003, by having fewer testicular follicles per testis. <i>Anaporrhutum mundae</i> sp. nov. also differs from <i>A. euzeti</i> in its forebody shape and by having different morphology and location of the vitellaria. The study of the tegumental surface of <i>A. mundae</i> sp. nov., as revealed by scanning electron microscopy, allowed detection of new morphological characters for a member of Anaporrhutinae that may be of taxonomic value. These are: a stylet cavity dorsal to the oral sucker with a large penetration gland opening on each side of the cavity and small penetration gland openings located ventral to the stylet cavity, arranged in a circle around the mouth. This represents the first record of an <i>Anaporrhutum</i> species from Costa Rica. Further, <i>A. mundae</i> sp. nov. represents the first parasite described or reported in this host.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140184596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acquisition and transmission of <i>Grapevine fanleaf virus</i> (GFLV) by <i>Xiphinema index</i> and <i>Xiphinema italiae</i> (Longidoridae).","authors":"B M'rabet Samaali, A Loulou, A MougouHamdane, S Kallel","doi":"10.1017/S0022149X24000154","DOIUrl":"10.1017/S0022149X24000154","url":null,"abstract":"<p><p><i>Grapevine fanleaf virus</i> (GFLV) is one of the most severe virus diseases of grapevines, causing fanleaf degeneration that is transmitted by <i>Xiphinema index.</i> This paper aims to isolate <i>Xiphinema</i> species from Tunisian vineyard soil samples and assess their ability to acquire and transmit GFLV under natural and controlled conditions. Based on morphological and morphometric analyses, Tunisian dagger nematodes were identified as <i>X. index</i> and <i>Xiphinema italiae.</i> These results were confirmed with molecular identification tools using species-specific polymerase chain reaction primers. The total RNA of GFLV was extracted from specimens of <i>Xiphinema</i> and amplified based on real-time polymerase chain reaction using virus-specific primers. Our results showed that <i>X. index</i> could acquire and transmit the viral particles of GFLV. This nepovirus was not detected in <i>X. italiae</i>, under natural conditions; however, under controlled conditions, this nematode was able to successfully acquire and transmit the viral particles of GFLV.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140175043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activities of pumpkin seed oil against Biomphalaria alexandrina snails and the infective stages of Schistosoma mansoni with special emphasis on genotoxic and histopathological alterations.","authors":"S E Mohammed, H S Mossalem, R M Gad El-Karim, A T Morsy, A M Ammar","doi":"10.1017/S0022149X24000166","DOIUrl":"10.1017/S0022149X24000166","url":null,"abstract":"<p><p>Schistosomiasis is a serious health issue in tropical regions, and natural compounds have gained popularity in medical science. This study investigated the potential effects of pumpkin seed oil (PSO) on <i>Biomphalaria [B.] alexandrina</i> snails (Ehrenberg, 1831), <i>Schistosoma [S.] mansoni</i> (Sambon, 1907) miracidium, and cercariae. The chemical composition of PSO was determined using gas chromatography/mass spectrometry. A bioassay was performed to evaluate the effects of PSO on snails, miracidia, and cercariae. The results showed no significant mortality of B. alexandrina snails after exposure to PSO, but it caused morphological changes in their hemocytes at 1.0 mg/ml for 24 hours. PSO exhibited larvicidal activity against miracidia after 2 hours of exposure at a LC₅₀ of 618.4 ppm. A significant increase in the mortality rate of miracidia was observed in a dose- and time-dependent manner, reaching a 100% death rate after 10 minutes at LC₉₀ and 15 minutes at LC₅₀ concentration. PSO also showed effective cercaricidal activity after 2 hours of exposure at a LC₅₀ of 290.5 ppm. Histological examination revealed multiple pathological changes in the digestive and hermaphrodite glands. The PSO had genotoxic effects on snails, which exhibited a significant increase [p≤0.05] in comet parameters compared to the control. The findings suggest that PSO has potential as a molluscicide, miracidicide, and cercaricide, making it a possible alternative to traditional molluscicides in controlling schistosomiasis.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140175044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nematodes associated with terrestrial gastropod molluscs in Belgium and additional characterisation of <i>Pellioditis californica</i> and <i>P. hermaphrodita</i>.","authors":"M A Cortes, W Bert, M Couvreur, D De Waele, P R Singh","doi":"10.1017/S0022149X2400004X","DOIUrl":"10.1017/S0022149X2400004X","url":null,"abstract":"<p><p>A survey for slug- and snail-associated nematodes was conducted in forests, parks, botanical gardens, and nature reserves at 13 localities in Belgium to uncover more diversity of gastropod mollusc-associated nematodes and to characterise <i>Pellioditis</i> populations found in the country. A total of 319 slugs and snails belonging to nine species were examined. <i>Arion vulgaris</i> was the most commonly found mollusc species in this study (eight locations), and 19.4% of the examined mollusc specimens were found infected by nematodes. The highest prevalence of nematodes was observed in <i>Cornu aspersum</i> (60%) followed by <i>A. vulgaris</i> (34.8%), <i>Limax maximus</i> (28.6%), and <i>Cepaea</i> sp. (20%). Eleven nematode species belonging to eight families were isolated and identified from the mollusc hosts including <i>Alloionema appendiculatum</i>, <i>Angiostoma dentiferum</i>, <i>A. gandavense</i>, <i>Angiostrongylus vasorum</i>, <i>Cosmocerca longicauda</i>, <i>Panagrolaimus</i> cf. <i>subelongatus</i>, <i>Pellioditis californica</i>, <i>P. hermaphrodita</i>, <i>Rhabditis</i> sp., <i>Tetrameres</i> cf. <i>fissispina</i>, and <i>Troglostrongylus</i> cf. <i>brevior.</i><i>Pellioditis</i> was the most commonly found nematode genus (at nine localities) and <i>C. longicauda</i> and <i>P. californica</i> were reported in Belgium for the first time. Co-infections of more than one nematode species were observed in eight (2.5%) molluscs specimens. Most co-infections consisted of two nematode species. In one <i>A. vulgaris</i> specimen, a co-infection of three nematode species (<i>A. vasorum</i>, <i>P. hermaphrodita</i>, and <i>Tetrameres</i> cf. <i>fissispina</i>) was observed. Four <i>ex vivo</i> cultures of <i>P. californica</i> and six <i>ex vivo</i> cultures of <i>P. hermaphrodita</i> were established from single hermaphrodites, and both species were described based on light microscopy, scanning electron microscopy, and morphometric, morphological, and molecular data.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140175045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of <i>Saccocoelioides miguelmontesi</i> n. sp. (Digenea: Haploporidae) from characid fishes in the Iguazu River Basin based on morphological and molecular evidence.","authors":"M M Montes, Y Croci, J Barneche, W Ferrari, G Reig Cardarella, S Martorelli","doi":"10.1017/S0022149X24000117","DOIUrl":"10.1017/S0022149X24000117","url":null,"abstract":"<p><p>Here we describe a new species of the genus <i>Saccocoelioides</i> found parasitizing <i>Astyanax dissimilis</i> Garavello & Sampaio, <i>Psalidodon bifasciatus</i> (Garavello and Sampaio) and <i>Bryconamericus ikaa</i> Casciotta, Almirón & Azpelicueta from the Iguazu National Park, Misiones province, Argentina. <i>Saccocoelioides miguelmontesi</i> n. sp. was studied based on morphological and molecular (28S rDNA and COI mtDNA sequences) data. The COI mtDNA tree indicated that the specimens collected from the three fish hosts are conspecific, with an intragroup p-distance of 0%. The new species shows an intermediate morphological configuration between the diminutive and robust forms described for <i>Saccocoelioides</i> by Curran (2018). Although, in the 28S rDNA tree, it is placed in a well-supported clade with the two robust species analysed (<i>S. elongatus</i> and S<i>. magnus</i>; p-distance of 1 and 2%, respectively), it differs from the robust group by the range of body size, mature egg size, oral and ventral sucker size, sucker ratio, oral sucker to pharynx ratio, and post-cecal or post-testis/body length percentage. Our results led us to redefine the robust group as having eggs shorter or equal in length to the pharynx. <i>Saccocoelioides miguelmontesi</i> n. sp. the 10^th species reported from Argentina and the 7^th species within the robust group.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140110389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}