Journal of Helminthology最新文献

{"title":"New <i>Contracaecum</i> species (Nematoda, Anisakidae) parasitizing <i>Ardea cocoi</i> Linnaeus (Aves, Ardeidae) in a mixed infection: morphological and molecular studies in Argentina - CORRIGENDUM.","authors":"L E Garbin, M M Montes, N Arredondo, J Barneche, M Ibáñez Shimabukuro, M Moncada, J I Diaz","doi":"10.1017/S0022149X26101394","DOIUrl":"https://doi.org/10.1017/S0022149X26101394","url":null,"abstract":"","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e56"},"PeriodicalIF":1.6,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148054935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metacercariae of <i>Lecithocladium</i> (Trematoda: Hemiuridae) species in gelatinous zooplankters from Japanese coastal waters, with information of adult stages.","authors":"T Waki, H Sekine, M Nitta, S Toshino, T Takano, T Okawa, M Taru, T Ojima, M Ojima, Y Kondo, S Ohtsuka","doi":"10.1017/S0022149X26101230","DOIUrl":"10.1017/S0022149X26101230","url":null,"abstract":"<p><p>Between 2019 and 2025, metacercariae of the trematode genus <i>Lecithocladium</i> (Hemiuridae) were found in gelatinous zooplankters occurring in Japanese coastal waters. To identify these metacercariae and elucidate their life cycles, fish hosts were simultaneously examined for adult trematodes. Molecular analyses of the nuclear 28S ribosomal RNA (rRNA) and mitochondrial cytochrome <i>c</i> oxidase subunit 1 (COI) regions, comparing them with adult <i>Lecithocladium</i> species from fish hosts, confirmed the metacercariae as those of <i>L. magnacetabulum</i> and <i>L.</i> cf. <i>excisiforme.</i> Metacercariae of <i>L. magnacetabulum</i> were detected in the comb jelly <i>Beroe cucumis</i> and the jellyfish <i>Aequorea coerulescens</i>; adults of this species had previously been recorded in the Japanese butterfish <i>Psenopsis anomala</i> and the red seabream <i>Pagrus major.</i> For <i>L.</i> cf. <i>excisiforme</i>, metacercariae were detected in the arrow worm <i>Aidanosagitta crassa</i> and an adult in the whitetongue jack <i>Uraspis helvola.</i> These predatory gelatinous zooplankters are presumed to be the third (or higher) intermediate or paratenic hosts, given that their prey, planktonic copepods, are typically reported as the second intermediate hosts for hemiurid species. Furthermore, adults of two additional hemiurid species, <i>L. excisum</i> and <i>Ectenurus trachuri</i>, were also isolated from the fish hosts. Cumulatively, this work and previous research suggest that gelatinous zooplankters represent crucial transmission routes for hemiurid trematodes.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e48"},"PeriodicalIF":2.0,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148042807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New taxonomic insights into three cyathostomine nematodes of Sumatran elephant (<i>Elephas maximus sumatranus</i>).","authors":"K Dewi, M Hanafiah, G Animalesto, A Sayuti, Y S Fitriana, Y Apriyanti, Y Fahrimal, R Ferdian, F Firdaus, R Ridwan, R R Wahyuni, R Marwati, T R Ferasyi","doi":"10.1017/S0022149X26101540","DOIUrl":"10.1017/S0022149X26101540","url":null,"abstract":"<p><p>Elephants harbour intestinal nematode infections, particularly cyathostomine nematodes (Cyathostomini: Strongylidae). Monitoring helminths in rare and endemic species is crucial, as infections can contribute to population declines. The Sumatran elephant (<i>Elephas maximus sumatranus</i>), a subspecies of the Asian elephant, is native exclusively to the island of Sumatra, Indonesia. The studies on nematodes of Sumatran elephants remain limited. In this study, three cyathostomine nematodes not previously reported from Indonesia - <i>Quilonia renniei</i>, <i>Murshidia (Murshidia) falcifera</i>, and <i>M. (M.) murshida -</i> were redescribed using light microscopy and scanning electron microscopy. This approach enabled the observation of previously unreported morphological features of taxonomic significance and corrected existing errors. Our SEM observations showed for the first time the detailed morphology of the cephalic extremity and the bursa of <i>Murshidia (M.) falcifera</i>, and <i>M. (M.) murshida.</i> Additionally, we provide the first molecular data on the nuclear DNA region (ITS1, 5.8S, and ITS2) of <i>Q. renniei</i>, the first mitochondrial partial cytochrome c oxidase I (COI) sequences for <i>M. (M.) murshida</i> and <i>M.</i> (<i>M</i>.) <i>falcifera</i>, and newly generated COI data for <i>Q. renniei.</i> These sequences were used to construct a phylogenetic tree of the elephant nematodes belonging to the Tribe Cyathostomini. The ITS phylogeny supported the monophyly of <i>Murshidia</i> and <i>Quilonia</i> and placed <i>Khalilia sameera</i> as a basal lineage, whereas the COI dataset failed to resolve relationships at the generic level. The ITS tree also confirmed the identity of <i>Q. renniei</i>, which appeared as sister to <i>Q. africana.</i></p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e55"},"PeriodicalIF":2.0,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148042783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Re-description and molecular characterization of <i>Acanthobothrium minus</i> Tazerouti, Kechemir-Issad, and Euzet, 2009 (Onchoproteocephalidea, Onchobothriidae) from two skates (Elasmobranchii, Rajidae) off the Algerian Coast, Western Mediterranean - ERRATUM.","authors":"S Omari, H S Randhawa, F Tazerouti","doi":"10.1017/S0022149X26101370","DOIUrl":"https://doi.org/10.1017/S0022149X26101370","url":null,"abstract":"","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e50"},"PeriodicalIF":1.6,"publicationDate":"2026-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148042136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A modified 'Puncture-Aspiration-Injection-Re-aspiration' (PAIR) technique to treat Hepatic Cystic Echinococcosis: A retrospective case series and narrative review.","authors":"G Khalil, A El Khoury, C Salameh, E Diab, L Kaadi, K Semaan, G Prince, G Nawfal","doi":"10.1017/S0022149X26101503","DOIUrl":"10.1017/S0022149X26101503","url":null,"abstract":"<p><p>Percutaneous treatments (PTs) are established minimally invasive options for the management of hepatic cystic echinococcosis (HCE). Conventional techniques include puncture, aspiration, injection, and re-aspiration (PAIR), which relies on scolicidal agents, and puncture, aspiration, injection, and drainage (PAID), which uses prolonged catheter drainage to evacuate cyst contents. In this study, we describe our experience with a modified PAIR technique for the treatment of hepatic cystic echinococcosis. Eight patients with 19 cysts were initially included, and outcomes were analyzed in 6 patients with 9 cysts who had complete follow-up imaging.Under CT guidance, a transhepatic puncture of the cyst was performed. Partial aspiration was followed by injection of hypertonic saline. After cystography, an 8-French catheter was introduced to replace the 18-gauge needle, allowing controlled aspiration. The injection and aspiration sequence was repeated three to four times. The catheter was removed early after complete cyst content evacuation. In cysts larger than 10 cm, 95% ethanol was used as the scolicidal agent.Follow-up imaging showed a significant reduction in cyst volume. Outcomes achieved with this modified PAIR technique were compared with previously reported percutaneous HCE treatment series in the literature and appeared to fall within the range of outcomes reported in prior percutaneous series. Notably, this technique enabled successful treatment of a complicated cyst associated with a gastric fistula in a single patient.Percutaneous management of HCE continues to evolve, and modified techniques allow tailored treatment based on cyst characteristics. Our modified PAIR approach proved effective for both simple and multivesicular cysts and demonstrated technical feasibility in a single complicated case with gastric fistula.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e51"},"PeriodicalIF":1.6,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148031392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, risk factors, and zoonotic potential of gastrointestinal helminths in cats: A participatory epidemiological study - CORRIGENDUM.","authors":"S Avachare, M Singh, M Shakya, A K Sharma, G N Bhangale, A Mourya, G P Jatav, N S Choudhary","doi":"10.1017/S0022149X26101369","DOIUrl":"https://doi.org/10.1017/S0022149X26101369","url":null,"abstract":"","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e49"},"PeriodicalIF":1.6,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148031400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helminth egg discoveries at the Letchworth archaeological site, Florida, USA, show evidence for animal resource use during c. 400 BC-AD 800.","authors":"M Horrocks, M Menz, B Presswell","doi":"10.1017/S0022149X26101576","DOIUrl":"10.1017/S0022149X26101576","url":null,"abstract":"<p><p>Here we report microfossil helminth analysis of 18 soil/sediment samples from the Letchworth site, Florida, dating c. 400 BC-AD 800 in the Woodland Period. The discovery of helminth eggs of <i>Toxocara canis</i>, <i>Dipylidium caninum</i>, a species of taeniid cestode, and <i>Hymenolepis</i> sp. provides probable evidence of domesticated dogs, and possibly also commensal rodents that could have been hunted or attracted to settlement. The parasites could have affected the health of people and their dogs. Helminths provide crucial faunal evidence at the site, as there is a lack of macro-remains due to poor preservation. The relatively high frequency of <i>T. canis</i> eggs at Letchworth (11 samples) is consistent with that at the Cloudman site in Michigan (another North American site where <i>T.</i><i>canis</i> helminths have been identified), where all samples analysed (12) contained these eggs, while the other helminth types were recorded in fewer samples. This consistency could reflect extensive, intensive human-dog associations for long periods in North America, in conjunction with direct faunal evidence for dogs and other parasitological evidence indicating <i>T. canis.</i> The Letchworth results offer a new line of probable evidence of domesticated dogs in the American Southeast, particularly along the Gulf Coast where bone material is often poorly preserved.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e53"},"PeriodicalIF":2.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148031384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adding another piece to the Camallanidae puzzle: description of <i>Denticamallanus peruensis</i> n. sp. from <i>Centromochlus heckelii</i> in the Peruvian Amazon, with implications for the monophyly of <i>Denticamallanus</i> Moravec & Thatcher, 1997.","authors":"G Morey, H A Davila, E V Ramirez, S Panduro, N Tarmeño, N Miranda, J D Chero","doi":"10.1017/S0022149X2610159X","DOIUrl":"10.1017/S0022149X2610159X","url":null,"abstract":"<p><p>During a helminthological survey conducted in the Peruvian Amazon, nematodes representing an undescribed species of <i>Denticamallanus</i> Moravec and Thatcher, 1997 (Camallanidae: Procamallaninae) were recovered from the intestine of <i>Centromochlus heckelii</i> (De Filippi, 1853) (Siluriformes: Auchenipteridae). The new species, <i>Denticamallanus peruensis</i> n. sp., is described herein based on an integrative taxonomic approach combining detailed morphological analysis using light and scanning electron microscopy with molecular characterization of the partial 18S rDNA gene. <i>Denticamallanus peruensis</i> n. sp. is distinguished from its congeners by the morphology of the male caudal extremity, which forms broad lateral lobes giving a spade-like appearance, the arrangement of caudal papillae (one pair of preanal and four pairs of postanal papillae), and spicule length (50-100 μm). Phylogenetic analyses based on Bayesian inference methods recovered the new species within a well-supported clade of <i>Spirocamallanus</i> spp., whereas other species currently assigned to <i>Denticamallanus</i> formed a separate lineage, indicating possible non-monophyly of the genus. This study represents the first description of a <i>Denticamallanus</i> species from <i>C. heckelii</i> in the Peruvian Amazon and increases the number of recognized species within the genus. In addition, these findings challenge the expanded generic diagnosis recently proposed for <i>Denticamallanus</i> and reinforce growing evidence that buccal capsule morphology alone may not reliably reflect evolutionary relationships within Camallanidae.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e54"},"PeriodicalIF":2.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148031418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative morphological and molecular characterization of Proterodiplostominae metacercariae (Digenea: Diplostomidae) infecting siluriform fishes in the Peruvian Amazon Basin.","authors":"N Huaman, L Ñacari, J Silvera, N Miranda, J Yunis-Aguinaga, G Morey, D Lopez, C L Cruces, J L Luque, J D Chero","doi":"10.1017/S0022149X26101552","DOIUrl":"10.1017/S0022149X26101552","url":null,"abstract":"<p><p>Proterodiplostominae Dubois, 1936 is a small but evolutionarily significant lineage of diplostomid trematodes that primarily parasitize crocodilians, with fishes acting as second intermediate or paratenic hosts. In Peru, knowledge of this group has been limited to the record of adult specimens of <i>Proterodiplostomum longum</i> (Brandes, 1888) recovered from <i>Caiman crocodilus</i> Linnaeus, 1758 (Sauropsida: Alligatoridae) in the Loreto region, and molecular evidence linking larval and adult stages has been absent. The present study provides the first integrative morphological and molecular characterization of Proterodiplostominae metacercariae infecting siluriform fishes in the Peruvian Amazon Basin. Metacercariae were collected from <i>Hoplosternum littorale</i> (Hancock, 1828) (Callichthyidae) and <i>Pterygoplichthys pardalis</i> (Castelnau, 1855) (Loricariidae) and examined using light and scanning electron microscopy, together with partial 28S rDNA and mitochondrial cox1 sequences. Phylogenetic analyses of 28S rDNA demonstrated that three isolates clustered within a strongly supported clade containing reference sequences of <i>P. longum</i> from crocodilian hosts, with minimal genetic divergence (≤0.536%), confirming their conspecificity. This study therefore provides the first molecularly supported record of <i>P. longum</i> metacercariae in Peruvian fishes and clarifies a previously undocumented component of its life cycle. In addition, phylogenetic analyses revealed two distinct evolutionary lineages (designated herein as Proterodiplostominae gen. sp. 4 and gen. sp. 5) exhibiting 9.882% mitochondrial divergence, suggesting previously unrecognized diversity within the subfamily. These findings expand the known host range of <i>P. longum</i>, provide new genetic data for larval stages of Proterodiplostominae in the Neotropics, and highlight the importance of integrative approaches for resolving life-cycle stages and species boundaries in Diplostomidae.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e52"},"PeriodicalIF":2.0,"publicationDate":"2026-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148031447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The immuno-protective efficacy of <i>Trichinella spiralis</i> nano-cathepsin B proteinase antigen in murine trichinellosis.","authors":"N M Abou Hussien, W M El-Kersh, N S El-Nahas, I M Ali Moharam, A F Atia, M E Abd-El-Shafy Lasheen","doi":"10.1017/S0022149X2610145X","DOIUrl":"10.1017/S0022149X2610145X","url":null,"abstract":"<p><strong>Background: </strong><i>Trichinella spiralis</i> cathepsin B proteins (<i>Ts</i>CB) are highly antigenic molecules secreted by the parasite and represent promising candidates for vaccine development. Nanoliposomes are efficient and advanced drug delivery systems that enhance antigen stability and immunogenicity.</p><p><strong>Objective: </strong>This study aimed to evaluate the protective efficacy of <i>T. spiralis</i> cathepsin B proteinase antigen (<i>Ts</i>CBPA), either alone or loaded onto nanoliposomes, with or without aluminium hydroxide as an adjuvant, in a murine model of trichinellosis.</p><p><strong>Material and methods: </strong>Sixty male Swiss albino mice were divided into two main groups: a control (non-infected and <i>T. spiralis</i>-infected subgroups) and a vaccinated groups, the later subdivided into four subgroups according to vaccination protocols. Parasitological assessment was performed on the 8th day post-infection (dpi) to evaluate adult worm burden and on the 35th dpi to assess larval burden. Histopathological examination was conducted during both the intestinal and muscular phases. Enzyme-linked immunosorbent assay (ELISA) was used to measure circulating larval antigen, immunoglobulin M (IgM), and immunoglobulin G1 (IgG1). Real-time polymerase chain reaction (PCR) was performed to quantify larval DNA in intestinal and muscular tissues.</p><p><strong>Results: </strong>Vaccinated groups showed significant reductions in adult worm and larval counts, accompanied by improved inflammatory responses. Circulating larval antigen levels and larval DNA quantities were markedly reduced, while serum IgM and IgG1 levels were significantly increased. The highest protective efficacy was observed in mice vaccinated with nanoliposome-loaded <i>Ts</i>CBPA combined with aluminium hydroxide.</p><p><strong>Conclusion: </strong><i>Ts</i>CBPA and its nanoliposome formulation showed strong protection against murine trichinellosis, with aluminium hydroxide significantly enhancing vaccine efficacy.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e47"},"PeriodicalIF":2.0,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147987826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}