Journal of Helminthology最新文献

{"title":"A new species of lungworm from the Atlantic Forest: Rhabdias megacephala n. sp. parasite of the endemic anuran Proceratophrys boiei","authors":"R. Euclydes, F.T. de Vasconcelos Melo, H.C. da Justa, R.F. Jesus, L.H. Gremski, S.S. Veiga, K.M. Campião","doi":"10.1017/s0022149x24000385","DOIUrl":"https://doi.org/10.1017/s0022149x24000385","url":null,"abstract":"<jats:italic>Rhabdias</jats:italic> are lung-dwelling parasites of anurans and some reptiles. Currently, 93 species are known to exist worldwide. The identification of <jats:italic>Rhabdias</jats:italic> species is based mainly on morphological traits of hermaphroditic females that generally have a very conserved morphology. However, different approaches, such as the combination of morphological, molecular, and ecological data, have provided advances in identifying and delimiting rhabdiasid species. Here, we describe a new species of <jats:italic>Rhabdias</jats:italic> from the south of Brazil, with morphological and molecular data. The new species is distinguished from its congeners by having an elongated body, evident cephalic dilation, larger buccal capsule, and large esophagus. In addition to morphological characteristics, we observed significant genetic divergence among the cytochrome oxidase subunit I (COI) sequence of the new species and the closest available sequence, <jats:italic>Rhabdias fuelleborni</jats:italic> (10.24%–10.87%). Furthermore, phylogenetic reconstructions based on the COI gene indicated that the new species represents a different lineage, constituting an outgroup of the species complexes <jats:italic>Rhabdias</jats:italic> cf. <jats:italic>stenocephala</jats:italic> and <jats:italic>Rhabdias fuelleborni</jats:italic> with <jats:italic>Rhabdias</jats:italic> sp. 4. Thus, <jats:italic>Rhabdias megacephala</jats:italic> is the 24th nominal species of the Neotropical region, the 14th Brazilian, and the fourth species described from south of Brazil.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"211 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142253278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trematode species diversity in the faucet snail, Bithynia tentaculata at the western edge of its native distribution, in Ireland","authors":"A. Faltýnková, K. O’Dwyer, C. Pantoja, D. Jouet, K. Skírnisson, O. Kudlai","doi":"10.1017/s0022149x24000397","DOIUrl":"https://doi.org/10.1017/s0022149x24000397","url":null,"abstract":"Trematodes and their snail hosts have developed intimate parasite-host associations, with snails supporting a diverse and often species-specific trematode fauna. In the faucet snail, <jats:italic>Bithynia tentaculata</jats:italic> (Caenogastropoda, Littorinimorpha), a unique trematode fauna has been recorded recently. However, knowledge of the exact species identity, phylogenetic relationships, and geographical distribution remains limited as many of the species belong to groups with unclear or controversial taxonomical assignment. To contribute to our knowledge of the trematodes, we investigated the trematode fauna of <jats:italic>B. tentaculata</jats:italic> by examining a total of 556 snails from lakes in County Galway, Ireland. Using an integrative taxonomic approach including DNA sequence data analyses (28S rRNA gene, ITS1-5.8S-ITS2, ITS2, <jats:italic>cox</jats:italic>1, <jats:italic>nad</jats:italic>1) and morphological tools (taxonomical drawings and measurements), we identified nine trematode species of seven families, with seven species occurring as cercariae (<jats:italic>Cyathocotyle prussica</jats:italic>, <jats:italic>Lecithodendrium linstowi</jats:italic>, <jats:italic>Lecithodendrium</jats:italic> sp., <jats:italic>Asymphylodora progenetica</jats:italic>, <jats:italic>Sphaerostoma bramae</jats:italic>, <jats:italic>Metorchis xanthosomus</jats:italic>, and <jats:italic>Notocotylus</jats:italic> sp.) and three species occurring as metacercariae (<jats:italic>A. progenetica</jats:italic>, <jats:italic>Parasymphylodora parasquamosa</jats:italic>, and <jats:italic>Sphaeridiotrema</jats:italic> sp.). Except for <jats:italic>S. bramae</jats:italic>, all are new species records for Ireland and provide the most western distribution of these trematodes in Europe. The trematode species recorded are known to use a wide range of definitive hosts and have a wide geographical distribution; among them are species members of genera that are zoonotic (<jats:italic>Metorchis</jats:italic>) and pathogenic to wildlife (<jats:italic>Cyathocotyle</jats:italic>, <jats:italic>Sphaeridiotrema</jats:italic>, and <jats:italic>Notocotylus</jats:italic>). There remains an ongoing need for precise identification of the trematode species to ensure that wider ecological contexts are correctly understood and biodiversity and disease threats can be accurately evaluated.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"5 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142253276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An automated faecal egg count system for detection of <i>Ascaridia galli</i> ova in chickens.","authors":"J L Cain, D Wilson, P Slusarewicz","doi":"10.1017/S0022149X24000373","DOIUrl":"https://doi.org/10.1017/S0022149X24000373","url":null,"abstract":"<p><p>Chicken production has increased over the past decade, resulting in a concomitant rise in the demand for more humane options for poultry products including cage-free, free-range, and organic meat and eggs. These husbandry changes, however, have come hand-in-hand with increased prevalence of <i>Ascaridia galli</i> infection, which can cause clinical disease in chickens as well as the occasional appearance of worms in eggs. Additionally, development of anthelmintic resistance in closely related helminths of turkeys highlights the need for closely monitored anthelmintic treatment programs. Manual faecal egg counts (FECs) can be time-consuming and require specialist training. As such, this study sought to validate an automated FEC system for use in detection and quantification of <i>A. galli</i> eggs in chicken faeces. Automated counts using the Parasight System (PS) were compared to traditional manual McMaster counting for both precision and correlation between methods. Overall, ten repeated counts were performed on twenty individual samples for a total of 200 counts performed for each method. A strong, statistically significant correlation was found between methods (R² = 0.7879, <i>P</i> < 0.0001), and PS counted more eggs and performed with statistically significant higher precision (<i>P</i> = 0.0391) than manual McMaster counting. This study suggests that PS is a good alternative method for performing <i>A. galli</i> FECs and provides a new tool for use in helminth treatment and control programs in chicken operations.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"98 ","pages":"e49"},"PeriodicalIF":1.3,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metazoan endoparasites of snakes from Argentina: Review and checklist with distributional notes and remarks.","authors":"Juan Nicolás Caraballo, Andrés Ulibarrie, Monika Hamann, Ricardo Guerrero, Vanesa Arzamendia, Cynthya Elizabeth González","doi":"10.1017/S0022149X24000130","DOIUrl":"https://doi.org/10.1017/S0022149X24000130","url":null,"abstract":"<p><p>This article presents a checklist of metazoan parasites of snakes from Argentina, along with a comprehensive review of the relevant literature published between 1922 and June 2023, covering various aspects of interest. We compiled 34 species of metazoan endoparasites from 28 studies. The subclass Digenea showed the highest number of species (n = 22 species), followed by the phylum Nematoda (n = 8 species), and the subclass Pentastomida (n = 3 species and 1 <i>taxa inquirenda</i>). Dipsadidae was the family of snakes with the most species examined for metazoan endoparasites (n = 20 species). In contrast, Viperidae had the largest number of specimens surveyed (n = 343). Of 23 provinces, 15 (65.2%) presented at least one report of metazoan endoparasites in snakes. The northeastern provinces showed the highest richness of metazoan endoparasites and host diversity. Many articles focused on taxonomy, but studies on parasite ecology were not found. Although taxonomic accuracy was high in most reports, some records were correctly deposited in zoological collections or geo-referenced. This is the first attempt to include all groups of metazoan endoparasites of snakes from Argentina in a single checklist in the last century.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"98 ","pages":"e48"},"PeriodicalIF":1.3,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematics and life cycles of four avian schistosomatids from Southern Cone of South America.","authors":"P Oyarzún-Ruiz, R Thomas, A Santodomingo, M Zamorano-Uribe, M Moroni, L Moreno, S Muñoz-Leal, V Flores, S Brant","doi":"10.1017/S0022149X2400035X","DOIUrl":"https://doi.org/10.1017/S0022149X2400035X","url":null,"abstract":"<p><p>Relative to the numerous studies focused on mammalian schistosomes, fewer include avian schistosomatids particularly in the southern hemisphere. This is changing and current research emerging from the Neotropics shows a remarkable diversity of endemic taxa. To contribute to this effort, nine ducks (<i>Spatula cyanoptera</i>, <i>S.</i><i>versicolor</i>, <i>Netta peposaca</i>), 12 swans (<i>Cygnus melancoryphus</i>) and 1,400 <i>Physa</i> spp. snails from Chile and Argentina were collected for adults and larval schistosomatids, respectively. Isolated schistosomatids were preserved for morphological and molecular analyses (<i>28S</i> and <i>COI</i> genes). Four different schistosomatid taxa were retrieved from birds: <i>Trichobilharzia</i> sp. in <i>N. peposaca</i> and <i>S. cyanoptera</i> that formed a clade; <i>S.</i><i>cyanoptera</i> and <i>S. versicolor</i> hosted <i>Trichobilharzia querquedulae</i>; <i>Cygnus melancoryphus</i> hosted the nasal schistosomatid, <i>Nasusbilharzia melancorhypha</i>; and one visceral, Schistosomatidae gen. sp., which formed a clade with furcocercariae from Argentina and Chile from previous work. Of the physid snails, only one from Argentina had schistosomatid furcocercariae that based on molecular analyses grouped with <i>T. querquedulae.</i> This study represents the first description of adult schistosomatids from Chile as well as the elucidation of the life cycles of <i>N.</i><i>melancorhypha</i> and <i>T. querquedulae</i> in Chile and Neotropics, respectively. Without well-preserved adults, the putative new genus Schistosomatidae gen. sp. could not be described, but its life cycle involves <i>Chilina</i> spp. and <i>C. melancoryphus.</i> Scanning electron microscopy of <i>T. querquedulae</i> revealed additional, undescribed morphological traits, highlighting its diagnostic importance. Authors stress the need for additional surveys of avian schistosomatids from the Neotropics to better understand their evolutionary history.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"98 ","pages":"e47"},"PeriodicalIF":1.6,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141200004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiology and diversity of gastrointestinal tract helminths of wild ruminants in sub-Saharan Africa: a review.","authors":"V Phetla, M Chaisi, M P Malatji","doi":"10.1017/S0022149X24000361","DOIUrl":"10.1017/S0022149X24000361","url":null,"abstract":"<p><p>This review summarises studies on distribution, diversity, and prevalence of gastrointestinal helminth infections in wild ruminants in sub-Saharan Africa. The results showed that 109 gastrointestinal tract (GIT) helminth species or species complexes were recorded in 10 sub-Saharan African countries. South Africa reported the highest number of species because most studies were carried out in this country. Eighty-eight nematode species or species complexes were recorded from 30 wild ruminant species across eight countries. The genus <i>Trichostrongylus</i> recorded the highest number of species and utilised the highest number of wild ruminant species, and along <i>with Haemonchus</i> spp., was the most widely distributed geographically. Fifteen trematode species or species complexes were reported from seven countries. The genus <i>Paramphistomum</i> recorded the highest number of species, and <i>Calicophoron calicophoron</i> was the most commonly occurring species in sub-Saharan African countries and infected the highest number of hosts. Six cestode species or species complexes from one family were documented from 14 wild hosts in seven countries. <i>Moniezia</i> spp. were the most commonly distributed in terms of host range and geographically. Impala were infected by the highest number of nematodes, whilst Nyala were infected by the highest number of trematode species. Greater kudu and Impala harbored the largest number of cestodes. The prevalence amongst the three GIT helminths taxa ranged between 1.4% and 100% for nematodes, 0.8% and 100% for trematodes, and 1.4% and 50% for cestodes. There is still limited information on the distribution and diversity of GIT helminths in wild ruminants in most sub-Saharan African countries.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"98 ","pages":"e45"},"PeriodicalIF":1.6,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141200002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cestode diversity in shrews from islands in the Sea of Japan and the Sea of Okhotsk.","authors":"S A Kornienko, N E Dokuchaev","doi":"10.1017/S0022149X24000324","DOIUrl":"https://doi.org/10.1017/S0022149X24000324","url":null,"abstract":"<p><p>A comparative analysis of taxonomic diversity on shrew cestodes among four islands in the Sea of Japan and the Sea of Okhotsk (Sakhalin, Kunashir, Hokkaido, and Moneron) was performed. Cestode species shared among the islands were identified and their host specificity was investigated. On Sakhalin Island, 33 species of the families Hymenolepididae, Dilepididae and Mesocestoididae were recorded in four shrew species (<i>Sorex caecutiens</i>, <i>S. gracillimus</i>, <i>S. minutissimus</i> and <i>S. unguiculatus</i>). In <i>S. caecutiens</i>, <i>S. gracillimus</i>, and <i>S. unguiculatus</i> on Kunashir Island, 22 species of the same families were found and, on Hokkaido Island, 23 species of the families Hymenolepididae and Dilepididae were recorded. On Moneron Island, three species of cestodes were registered in <i>S. tundrensis.</i> The Sakhalin-Hokkaido-Kunashir complex of shrew cestodes includes eastern-Palearctic, trans-Palearctic and endemic species. High endemism (~22%) of shrew tapeworms in the Sakhalin-Kunashir-Hokkaido Islands was noted as compared to continental territories. The different numbers of cestode species in <i>S. unguiculatus</i> (31), <i>S. caecutiens</i> (29), <i>S. gracillimus</i> (19) and <i>S. minutissimus</i> (1) were found. It was concluded that the cestodes species diversity of shrews of Sakhalin-Kunashir-Hokkaido depended primarily on the history of island formation, their modern physical and geographical features, the abundance of definitive and intermediate cestodes hosts and, to a lesser extent, on the size and remoteness of the islands from the mainland and the diversity of host species.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"98 ","pages":"e46"},"PeriodicalIF":1.6,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141200069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogeny and morphology of some European cyathocotylid digeneans (Trematoda: Diplostomoidea).","authors":"S G Sokolov, S A Vlasenkov, S V Bugmyrin, A P Kalmykov, D I Lebedeva","doi":"10.1017/S0022149X24000348","DOIUrl":"https://doi.org/10.1017/S0022149X24000348","url":null,"abstract":"<p><p>The Cyathocotylidae Mühling, 1898 is a family of primitive diplostomoid trematodes important for understanding the evolution of the superfamily Diplostomoidea. However, cyathocotylids remain poorly studied with the use of molecular techniques. In this study we sequenced the 5.8S + ITS2 region, <i>28S rRNA</i>, and <i>cox1</i> genes of two cyathocotylid species and obtained new morphological data on them. We propose <i>Georduboisia</i> nom. nov. instead of the preoccupied name <i>Duboisia</i> Szidat, 1936 (junior homonym of <i>Duboisia</i> Stremme, 1911). Adults of <i>Georduboisia</i> cf. <i>teganuma</i> (Ishii, 1935) and <i>Paracoenogonimus ovatus</i> Katsurada, 1914 were collected from fish-eating birds in the south of the European part of Russia. <i>Georduboisia</i> cf. <i>teganuma</i> was very similar to <i>G.</i><i>teganuma</i> but differed from it in the shape of the testes. The <i>28S rRNA</i> gene dataset provided the best-resolved phylogeny of the Cyathocotylidae to date. In the phylogram based on partial sequences of this gene, <i>P. ovatus</i> was close to members of <i>Holostephanoides</i> Dubois, 1983, <i>Neogogatea</i> Chandler & Rausch, 1947 and <i>Gogatea</i> Szidat, 1936. <i>Georduboisia</i> cf. <i>teganuma</i> clustered with members of <i>Cyathocotyle</i> Mühling, 1896 and <i>Holostephanus</i> Szidat, 1936. Phylogenetic analysis based on the 5.8S + ITS2 dataset showed that adults of <i>P. ovatus</i> examined in our study were conspecific with the metacercariae from the musculature of fish collected in Hungary and Italy. It also revealed probable misidentifications of larvae and adults of cyathocotylids whose sequences are deposited in GenBank NCBI.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"98 ","pages":"e44"},"PeriodicalIF":1.6,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol potential of six <i>Heterorhabditis bacteriophora</i> strains isolated in the Azores Archipelago.","authors":"R Beltrí, H R Monteiro, D Toubarro, N Simões, A Garriga","doi":"10.1017/S0022149X24000336","DOIUrl":"https://doi.org/10.1017/S0022149X24000336","url":null,"abstract":"<p><p>Entomopathogenic nematodes (EPNs) are closely associated with <i>Popillia japonica</i> and potentially used as their biological control agents, although field results proved inconsistent and evoked a continual pursuit of native EPNs more adapted to the environment. Therefore, we surveyed the Azorean Archipelago to isolate new strains of <i>Heterorhabditis bacteriophora</i> and to evaluate their virulence against the model organism <i>Galleria mellonella</i> under laboratory conditions. Six strains were obtained from pasture and coastal environments and both nematode and symbiont bacteria were molecularly identified. The bioassays revealed that Az172, Az186, and Az171 presented high virulence across the determination of a lethal dose (LD50) and short exposure time experiments with a comparable performance to Az29. After 72 hours, these virulent strains presented a mean determination of a lethal dose of 11 infective juveniles cm^-2, a lethal time (LT50) of 34 hours, and achieved 40% mortality after an initial exposure time of only 60 minutes. Az170 exhibited an intermediate performance, whereas Az179 and Az180 were classified as low virulent strains. However, both strains presented the highest reproductive potential with means of 1700 infective juveniles/mg of larvae. The bioassays of the native EPNs obtained revealed that these strains hold the potential to be used in biological control initiatives targeting <i>P. japonica</i> because of their high virulence and locally adapted to environmental conditions.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"98 ","pages":"e43"},"PeriodicalIF":1.6,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Data on four species of <i>Longidorus</i> Micoletzky, 1922 (Nematoda: Longidoridae) from southern and southeastern Iran, including description of a new species.","authors":"S Monemi, M Behdani, E Mahdikhani-Moghadam, N Amirzadi, M R Atighi, W Ye, F Jahanshahi Afshar, M Pedram","doi":"10.1017/S0022149X24000282","DOIUrl":"https://doi.org/10.1017/S0022149X24000282","url":null,"abstract":"<p><p>Four species of the genus <i>Longidorus</i> were recovered from southern (Bushehr province) and southeastern (Southern Khorasan province) Iran. The first species, <i>L. paratabrizicus</i> n. sp. represents a new member to the genus and is characterised by 4.8-5.6 mm long females with anteriorly flattened lip region separated from the rest of the body by depression, amphidial fovea pocket-shaped without lobes, tail conical, dorsally convex, ventrally almost straight with bluntly rounded tip and males in population. By having similar lip region and tail shape, the new species most closely resembles five species <i>viz. L. artemisiae, L. globulicauda, L. patuxentensis, L. sturhani</i>, and <i>L. tabrizicus</i>. It represents the cryptic form of the last species. The second species belongs to <i>L. mirus</i>, recovered in both southern and southeastern Iran, representing the first record of the species after its original description. As an update to the characteristics of this species, it's all juvenile developmental stages were recovered and described. The criteria to separate L. mirus from two closely related species, <i>L. auratus</i> and <i>L. africanus</i>, are discussed. The third species belongs to <i>L. persicus</i>, a new record in southern Iran. The fourth species, <i>L. orientalis</i> was recovered in high population density in association with date palm trees in Bushehr province. The phylogenetic relationships of the new species and recovered populations of <i>L. mirus</i> and <i>L. persicus</i> were reconstructed using two ribosomal markers and the resulted topologies were discussed.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"98 ","pages":"e42"},"PeriodicalIF":1.6,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}