Journal of Helminthology最新文献_第4页

Phylogenetic relationships of Posthodiplostomum sp. metacercarial morphotype (Diplostomidae) infecting four Pampean rivulid genera. 潘潘亚四种鲤科昆虫后棘口蝇属囊蚴形态的系统发育关系。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-18 DOI: 10.1017/S0022149X26101862

M M Montes, J Barneche, T Acuña González, I Garcia, F Alonso, M Ibáñez Shimabukuro, G Reig Cardarella

{"title":"Phylogenetic relationships of Posthodiplostomum sp. metacercarial morphotype (Diplostomidae) infecting four Pampean rivulid genera.","authors":"M M Montes, J Barneche, T Acuña González, I Garcia, F Alonso, M Ibáñez Shimabukuro, G Reig Cardarella","doi":"10.1017/S0022149X26101862","DOIUrl":"https://doi.org/10.1017/S0022149X26101862","url":null,"abstract":"Morphologically similar diplostomid metacercariae were found infecting four species of Pampean rivulid fishes - Austrolebias bellottii, Amatolebias patriciae, Argolebias nigripinnis, and Titanolebias elongatus - from temporary freshwater ponds in the Pampean region of Argentina. Although these larvae occur in different host species and localities, comparative morphological analyses revealed extensive overlap in diagnostic characters. To assess whether this morphological similarity reflected conspecificity, molecular analyses based on partial 28S rDNA and ITS2 rDNA sequences were performed. Phylogenetic reconstructions recovered all metacercariae from rivulid hosts as a single, well-supported lineage, consistent with their assignment to a single lineage Posthodiplostomum species. Genetic distances among ITS2 sequences were zero, indicating complete sequence identity across hosts and localities. Infection parameters varied among host species and populations, with Austrolebias bellottii and Titanolebias elongatus exhibiting the highest values. Differences in infection levels could reflect variation in local environmental conditions, habitat characteristics, and the availability of avian definitive hosts, potentially influencing parasite transmission dynamics. This study provides integrative morphological and molecular evidence consistent with all metacercariae representing a single Posthodiplostomum lineage infecting multiple Pampean rivulid species and contributes to a better understanding of parasite diversity and transmission patterns in South American temporary ponds.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e108"},"PeriodicalIF":2.0,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complexity in the recognition of species of Hexangium Goto & Ozaki, 1929 (Trematoda: Microscaphidiidae) from Indo-Pacific Siganidae. Goto & Ozaki, 1929（吸虫目：微蝇科）在印度-太平洋象形虫科中物种识别的复杂性。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-18 DOI: 10.1017/S0022149X26101825

T H Cribb, R A Bray, S B Martin, N Q-X Wee, S C Cutmore

{"title":"Complexity in the recognition of species of Hexangium Goto & Ozaki, 1929 (Trematoda: Microscaphidiidae) from Indo-Pacific Siganidae.","authors":"T H Cribb, R A Bray, S B Martin, N Q-X Wee, S C Cutmore","doi":"10.1017/S0022149X26101825","DOIUrl":"https://doi.org/10.1017/S0022149X26101825","url":null,"abstract":"Species of Hexangium (Microscaphidiidae) were collected from 12 species of rabbitfishes (Siganidae) from multiple Indo-West Pacific locations: three on the Great Barrier Reef in Queensland, two in south-east Queensland, Ningaloo Reef in Western Australia, New Caledonia and Japan; no specimens were found from Palau or French Polynesia. Parallel molecular (cox1 mtDNA, ITS2 and 28S rDNA) and morphological analyses, together with consideration of host and geographical distribution, lead us to recognise six species. Specimens from Siganus fuscescens (Houttuyn) from Japan are identified as the type species, H. sigani Goto & Ozaki, 1929, which was originally described from S. fuscescens from off Japan. Hexangium affinum Tubangui & Masiluñgan, 1944, originally described from Siganus javus (Linnaeus) from the Philippines but previously considered a synonym of H. sigani, is reinstated, and H. secundum Annereaux, 1947 is considered its junior synonym. Four new species are described: H. urabeae n. sp., H. kuramochii n. sp., H. robustum n. sp. and H. reimeri n. sp. Based on morphology alone, individual specimens of several combinations of these species are difficult to distinguish. Although none of the combinations of species are entirely mutually cryptic, we consider taxonomy of this genus to be dependent on molecular analysis; morphological distinction of species requires substantial numbers of well-preserved specimens. Most species of Siganus examined harbour two or three species of Hexangium; sometimes one species is dominant, but in several two species were both common. All six Hexangium species recognised here have been found in multiple locations; four species were found at each of two Great Barrier Reef sites.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e111"},"PeriodicalIF":2.0,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling helminth diversity in Scinax Wagler, 1830 (Anura: Hylidae): New records from S. x-signatus (Spix, 1824) in the Caatinga domain, Brazil, and an overview of South America. 揭示1830年sinax Wagler地区的蠕虫多样性（无尾目：Hylidae）：巴西Caatinga地区S. x-signatus （Spix, 1824）的新记录及南美洲概况。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-18 DOI: 10.1017/S0022149X26102132

F M Vieira, J R M Souza, D F Oliveira, C A C Carvalho, F S G Nascimento, D C N Silva, L B Ribeiro, L C Muniz-Pereira

{"title":"Unveiling helminth diversity in Scinax Wagler, 1830 (Anura: Hylidae): New records from S. x-signatus (Spix, 1824) in the Caatinga domain, Brazil, and an overview of South America.","authors":"F M Vieira, J R M Souza, D F Oliveira, C A C Carvalho, F S G Nascimento, D C N Silva, L B Ribeiro, L C Muniz-Pereira","doi":"10.1017/S0022149X26102132","DOIUrl":"https://doi.org/10.1017/S0022149X26102132","url":null,"abstract":"Amphibian helminth diversity remains poorly documented in semiarid environments, despite its relevance for understanding host-parasite interactions and biogeographical patterns. We investigated the helminth community of the hylid frog Scinax x-signatus from a Caatinga remnant in Petrolina (Pernambuco, Brazil) and reviewed the current state of knowledge on helminth infecting South American Scinax species. Twenty-five S. x-signatus (11 adult females, 9 adult males and 5 juveniles) were examined; of these, 13 specimens (52%) harboured at least 1 helminth taxon, with infracommunity richness ranging from 1 to 7 taxa. We identified four nematodes (Oswaldocruzia sp., Oxyascaris caudacutus, Oxysomatium sp., Rhabdias sp.), two digeneans (adults of Catadiscus sp. and metacercariae of Neodiplostomum sp., and cystacanths of an acanthocephalan (Centrorhynchus sp.). Oxyascaris caudacutus was the most prevalent taxon (20%) and exhibited the highest mean intensity (4.0 ± 2.77). Four taxa (Oswaldocruzia sp., O. caudacutus, Oxysomatium sp. and Catadiscus sp.) represent new host records for S. x-signatus. Our literature review identified 45 publications from 1940 to 2026 and showed that helminth have been recorded for only 13 South American Scinax species, comprising 78 taxa in total (46 Nematoda, 20 Trematoda, 8 Acanthocephala and 4 Cestoda). Records are strongly geographically biased, being restricted to five countries (Brazil, Argentina, Peru, Paraguay, and Ecuador), and most taxa remain identified only to genus or higher taxonomic levels. Our results expand knowledge of Caatinga amphibian parasites and reinforce the need for integrative surveys to better estimate helminth diversity across the South American continent.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e103"},"PeriodicalIF":2.0,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parachrisomon debonoae n. sp. (Digenea: Monorchiidae), a new species with significant intraspecific morphological variation infecting Mullidae (Mulliformes) from the eastern and western coasts of Australia. 澳洲东、西海岸Mullidae （Mulliformes）一新种，种内形态变异显著。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-18 DOI: 10.1017/S0022149X26102235

N Q-X Wee, S C Cutmore, S B Martin, R A Bray, T H Cribb

{"title":"Parachrisomon debonoae n. sp. (Digenea: Monorchiidae), a new species with significant intraspecific morphological variation infecting Mullidae (Mulliformes) from the eastern and western coasts of Australia.","authors":"N Q-X Wee, S C Cutmore, S B Martin, R A Bray, T H Cribb","doi":"10.1017/S0022149X26102235","DOIUrl":"https://doi.org/10.1017/S0022149X26102235","url":null,"abstract":"We report trematodes conforming to the generic concept of Parachrisomon Madhavi, 2008, infecting multiple species of goatfishes (Mullidae) from Moreton Bay (south-eastern Queensland) and off Lizard Island (northern Great Barrier Reef) on the eastern coast of Australia, and from Ningaloo Reef on the western coast. Initial morphological analyses suggested the possible presence of multiple species; however, no morphological distinctions proved consistent. Molecular analyses demonstrate a distinction between western and eastern Australian samples, especially in mt cox1 sequence data, but the differences are interpreted as intraspecific geographical variation. Specimens from all localities share a bipartite terminal organ with an irregularly shaped and lobed anterior part; the other eight species of Parachrisomon are reported with unspecialised, bipartite terminal organs. We recognise the specimens as representing a new species, Parachrisomon debonoae n. sp., that exhibits significant intraspecific morphological variation, especially in body size, pharynx size, and shape and size of the ovary and testis. Additionally, we report novel molecular data for Parachrisomon delicatus (Manter & Pritchard, 1961) Madhavi, 2008.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e115"},"PeriodicalIF":2.0,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal analysis of parasitic nematodes preserved in land snail shells. 保存在蜗牛壳中的寄生线虫的时空分析。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-18 DOI: 10.1017/S0022149X26102041

R Rae

{"title":"Spatiotemporal analysis of parasitic nematodes preserved in land snail shells.","authors":"R Rae","doi":"10.1017/S0022149X26102041","DOIUrl":"https://doi.org/10.1017/S0022149X26102041","url":null,"abstract":"Understanding how parasite abundance changes over time is inherently challenging, as trematodes, nematodes and cestodes degrade quickly and are poorly preserved. Here, I propose a novel approach for investigating the spatiotemporal dynamics of parasitic nematode infection by exploiting a key component of the snail immune system - the ability to trap and encapsulate these parasites within their shell. By utilising museum and modern collections, I was able to understand how nematode populations fluctuated in two species of snails (Cepaea nemoralis and C. hortensis) from three locations (Point of Ayr in Wales, Formby in England and Strathy beach in Scotland) from 1962 to 2024. I show that in vivo parasite populations collected from Point of Ayr in 1962 were initially low, with 8% of shells infected, but this dramatically increased to 60% in 2016 and fell to 10% in 2024. The number of C. nemoralis shells infected from Formby in 2016 was 18% but increased to 31% in 2024, and 27% of C. hortensis shells from Strathy beach in 2016 were infected by nematodes but plummeted to 3% in 2024. Thus, dramatic spatiotemporal changes in nematode abundance can be seen over several decades. By combining preserved snails in conchology collections with collections of modern specimens, empirical evidence of how parasite numbers change over time can be quantified.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e112"},"PeriodicalIF":2.0,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intra- and intertaxon similarity of nematode assemblages among sympatric rodents differing ecologically. 生态上不同的同域啮齿动物中线虫组合的类群内和类群间相似性。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-18 DOI: 10.1017/S0022149X26102077

E Schlemmer, L van der Mescht, C Matthee, S Matthee

{"title":"Intra- and intertaxon similarity of nematode assemblages among sympatric rodents differing ecologically.","authors":"E Schlemmer, L van der Mescht, C Matthee, S Matthee","doi":"10.1017/S0022149X26102077","DOIUrl":"https://doi.org/10.1017/S0022149X26102077","url":null,"abstract":"Ecological processes that shape animal assemblages act at multiple levels. Limited information is available on the factors that drive spatial variation in parasite communities, particularly in nematodes. This study aimed to reveal the role of host and environment as determinants of nematode species compositional similarity among three rodent taxa (Rhabdomys spp., Micaelamys namaquensis, and Otomys unisulcatus) that occur sympatrically in the Fynbos and Succulent Karoo biomes of the Western Cape Province of South Africa. In total, eight monoxenous and three heteroxenous nematode species were recorded. Seven species occurred in Rhabdomys spp., six in M. namaquensis, and five in O. unisulcatus. Nematode communities were more similar between Rhabdomys and Otomys, which also share comparable microhabitats. Nematodirus sp. and Neoheligmonella capensis, both with free-living larval stages, contributed strongly to between-host differences that were also correlated with low-lying habitat use. However, part of the between-host differences in species assemblages reflects host-restricted Syphacia spp. The influence of environmental factors on the composition of nematode infracommunities was also contingent on host life history. Overall, host diet, microhabitat use, as well as social and spatial behaviour appeared to exert a strong influence on the composition of nematode infracommunities. Results suggest that microhabitat use plays an important role in nematode community assembly, although direct environmental covariates were not formally analysed. Since between-host dissimilarities were influenced by host-restricted nematode species, habitat-driven patterns should be interpreted after accounting for host identity. The data generated here contribute to a broader understanding of the ecological niches preferred by parasitic nematodes of rodents.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e113"},"PeriodicalIF":2.0,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epidemiological screening of pinworm, schistosomiasis, and intestinal parasites in Eswatini preschoolers. 斯威士兰学龄前儿童蛲虫、血吸虫病和肠道寄生虫的流行病学筛查。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-18 DOI: 10.1017/S0022149X26102260

C-K Fan, C-M Chou, M N Mathobela, P-C Cheng, T-W Chuang

{"title":"Epidemiological screening of pinworm, schistosomiasis, and intestinal parasites in Eswatini preschoolers.","authors":"C-K Fan, C-M Chou, M N Mathobela, P-C Cheng, T-W Chuang","doi":"10.1017/S0022149X26102260","DOIUrl":"https://doi.org/10.1017/S0022149X26102260","url":null,"abstract":"Preschool children in the Kingdom of Eswatini are excluded from the national school-based deworming programme and had never been surveyed for parasitic infection. During June 2019 we conducted a cross-sectional baseline survey of 261 children (mean age 4.8 ± 0.75 years) attending 16 kindergartens and community care points across 4 provinces. Enterobius vermicularis was sought on peri-anal cellophane/Scotch-tape swabs (n = 261); Schistosoma haematobium in single urine specimens by the chamber-tube microscope-slide (CTMS) sedimentation method (n = 232); and intestinal helminths and protozoa in single stool specimens by the merthiolate-iodine-formaldehyde (MIF) concentration method (n = 175). Pinworm prevalence was 3.8% (10/261) and did not differ by sex (p = 0.76) or province (p = 0.83). S. haematobium ova were found in 0.9% (2/232), both in boys from recognised endemic foci. Intestinal parasites were detected in 41.1% (72/175), with no significant sex (p = 0.64) or regional (p = 0.70) variation; protozoa predominated (97.2% of positives) over helminths (4.2%; all Ascaris lumbricoides), the commonest species being Blastocystis hominis (61.1%) and Giardia lamblia (34.7%). These first baseline data reveal a high burden of intestinal protozoa alongside a low but measurable burden of pinworm and urogenital schistosomiasis in Eswatini preschool children, and support extending screening and preventive chemotherapy to this previously unmonitored age group using the simple, field-deployable cellophane-swab, CTMS and MIF methods.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e118"},"PeriodicalIF":2.0,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterisation and complete mitochondrial genome of Contracaecum ovale (Linstow, 1907) (Ascaridida: Ascaridoidea) from the little grebe Tachybaptus ruficollis (Pallas) (Podicipediformes: Podicipedidae). （足蜈蚣目：足蜈蚣科）小蜈蚣Tachybaptus ruficollis (Pallas)（蛔虫目：足蜈蚣科）的卵形缩螨（Linstow, 1907）（蛔虫目：蛔虫总科）的分子特征和全线粒体基因组。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-18 DOI: 10.1017/S0022149X2610203X

L-W Li, Z-J Hou, Y-Y Xie, L Li

{"title":"Molecular characterisation and complete mitochondrial genome of Contracaecum ovale (Linstow, 1907) (Ascaridida: Ascaridoidea) from the little grebe Tachybaptus ruficollis (Pallas) (Podicipediformes: Podicipedidae).","authors":"L-W Li, Z-J Hou, Y-Y Xie, L Li","doi":"10.1017/S0022149X2610203X","DOIUrl":"https://doi.org/10.1017/S0022149X2610203X","url":null,"abstract":"The genus Contracaecum is an important group of anisakid nematodes containing species of veterinary, medical, and economic importance. However, current genetic sequences and the mitogenomic database for Contracaecum spp. are limited. In the present study, the nuclear 18S, 28S, and ITS regions and the mitochondrial cox1, cox2, and 12S data for C. ovale (Linstow 1907) were sequenced based on the newly collected specimens from Tachybaptus ruficollis (Podicipediformes: Podicipedidae) in China. These sequences exhibited 100% similarity in the 18S and 28S regions and 99.8% similarity in the ITS-1 region (the presence of only a single locus of nucleotide polymorphism), compared with samples of Contracaecum ovale from Podiceps cristatus australis in New Zealand. Molecular analysis of two different individuals of C. ovale, collected from T. ruficollis in China, showed no genetic divergence with respect to the 18S, 28S, ITS, cox1, and 12S regions, but a low level of divergence in the cox2 sequences (0.73%). The complete mitogenome of C. ovale, presented for the first time, is 13,769 bp in length and includes 12 protein-coding genes, 22 tRNA genes, 2 ribosomal RNAs, and 2 non-coding regions. The mitogenome of C. ovale represents the smallest known anisakid mitogenome, and its overall A + T content (71.5%) is the highest level of A + T content of any anisakid. The mitogenomic phylogeny of those Contracaecum spp. whose mitochondrial protein-coding genes (PCGs) have been studied suggests that C. rudolphii and C. ogmorhini are more closely related to each other than either is to C. ovale. The genetic and mitogenomic data presented herein will be helpful for future studies on the population genetics and phylogenetics of Contracaecum spp.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e107"},"PeriodicalIF":2.0,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anthelmintic evaluation of traditional medicinal plants from Pakistan using Caenorhabditis elegans supported by phytochemical screening and thin-layer chromatographic profiling. 基于植物化学筛选和薄层色谱分析的巴基斯坦传统药用植物秀丽隐杆线虫的驱虫评价。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-17 DOI: 10.1017/S0022149X26102016

A Khan, S K Panda, M Yaghoobi, Q Ullah, H Hu, L Schoofs, W Luyten

{"title":"Anthelmintic evaluation of traditional medicinal plants from Pakistan using Caenorhabditis elegans supported by phytochemical screening and thin-layer chromatographic profiling.","authors":"A Khan, S K Panda, M Yaghoobi, Q Ullah, H Hu, L Schoofs, W Luyten","doi":"10.1017/S0022149X26102016","DOIUrl":"https://doi.org/10.1017/S0022149X26102016","url":null,"abstract":"Traditional systems of medicine have acted as a primary health care modality for resource-constrained regions of the world since time immemorial. This study investigates the anthelmintic activity of 17 ethnobotanically selected medicinal plants from the Swat region of Pakistan and characterises their phytochemical profiles. The anthelmintic activity of plant extracts prepared in five different solvents (hexane, acetone, ethanol, methanol, and water) was determined by using Caenorhabditis elegans as a model, followed by assessment of the phytochemistry as well as thin-layer chromatographic profiling of the acetone plant extracts. The anthelmintic results revealed that 48% of the extracts showed significant activity against C. elegans, with Ajuga bracteosa and Punica granatum extracts, followed by Berberis lyceum, Mentha longifolia, and Artemisia maritima, being the most potent. Among solvents, acetone proved to be the most effective extractant solvent. The phytochemical analysis and TLC profiling revealed various phytochemical groups in selected plant extracts. These findings support the significance of ethnomedicinal herbs in the discovery of novel antiparasitic drug candidates. To the best of our knowledge, this is the first study to document the anthelmintic properties of selected plants from the Swat region of Pakistan, along with phytochemical screening and TLC profiling. This study indicates that the plants with potent anthelmintic activity, such as A. maritima, M. longifolia, B. lyceum, A. bracteosa, and P. granatum, could be potential candidates for anthelmintic drug discovery.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e100"},"PeriodicalIF":2.0,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of ITS2 secondary structures for selected allocreadiid species (Digenea: Plagiorchiida: Gorgoderoidea) and their use in taxonomy and systematics. 异源异体种（Digenea: Plagiorchiida: Gorgoderoidea） ITS2二级结构的特征及其在分类学和系统学中的应用。

IF 2 3区生物学

Journal of Helminthology Pub Date : 2026-09-17 DOI: 10.1017/S0022149X26102193

K S Vainutis, A N Voronova, M Singh

{"title":"Characterisation of ITS2 secondary structures for selected allocreadiid species (Digenea: Plagiorchiida: Gorgoderoidea) and their use in taxonomy and systematics.","authors":"K S Vainutis, A N Voronova, M Singh","doi":"10.1017/S0022149X26102193","DOIUrl":"https://doi.org/10.1017/S0022149X26102193","url":null,"abstract":"Secondary structures of the internal transcribed spacer 2 (ITS2) region of ribosomal RNA (rRNA) were reconstructed for 46 species belonging to 12 genera of the family Allocreadiidae. Most structures exhibited the classical four-domain conformation typical of ITS2, with some notable variants. The C-domains of Bunodera acerinae and Allocreadium sp. 2 were divided into three branches. Structures of Creptotrema funduli and Pseudoparacreptotrema macroacetabulata showed unique conformations, with unusually variable A and B domains. Ten-nucleotide sequence variation at the 5' end of the ITS2 region correlates with phylogenetic relationships within the family. A dichotomous key based on ITS2 secondary-structure features is proposed, providing a novel, annotation-independent tool for species identification. Phylogenetic analysis based on ITS2 data is also presented, but we acknowledge the limitations of this marker for resolving deep relationships and interpret the resulting topology as a hypothesis complementary to 28S-based phylogenies. The presence of regulatory motifs and palindromic elements suggests that ITS2 functions as a primary hub-DNA product that accumulates regulatory information, potentially influencing RNA folding, stability, and transcription efficiency. ITS2 secondary structure analysis, combined with the proposed key, provides a powerful approach for understanding the evolution and systematics of this complex trematode family.","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"100 ","pages":"e101"},"PeriodicalIF":2.0,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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