Journal of Helminthology最新文献

{"title":"From the laboratory to the clinic: advancing helminthic therapy for type 2 diabetes.","authors":"Y Zhu, X Fei, R Wang, J Wang, M Liu, Y Zhang, X Li, J Xu, Q Zhao, X Zhang, K Chen, H Li","doi":"10.1017/S0022149X25100576","DOIUrl":"10.1017/S0022149X25100576","url":null,"abstract":"<p><p>The interaction of helminth infections with type 2 diabetes (T2D) has been a major area of research in the past few years. This paper, therefore, focuses on the systematic review of the effects of helminthic infections on metabolism and immune regulation related to T2D, with mechanisms through which both direct and indirect effects are mediated. Specifically, the possible therapeutic role of helminths in T2D management, probably mediated through the modulation of host metabolic pathways and immune responses, is of special interest. This paper discusses the current possibilities for translating helminth therapy from basic laboratory research to clinical application, as well as existing and future challenges. Although preliminary studies suggest the potential for helminth therapy for T2D patients, their safety and efficacy still need to be confirmed by larger-scale clinical studies.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e86"},"PeriodicalIF":1.6,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cryphodera guangdongensis</i> n. sp., (Nematoda: Heteroderidae), a new species of cystoid nematode from roots and surrounding soil of <i>Schima superba</i> in Guangdong, China.","authors":"C-H Ni, W-H Chen, R Zhang, H-H Cai, Q-Y Li, C-L Xu, H Xie","doi":"10.1017/S0022149X25100412","DOIUrl":"https://doi.org/10.1017/S0022149X25100412","url":null,"abstract":"<p><p><i>Cryphodera guangdongensis</i> n. sp. was collected from the soil and roots of <i>Schima superba</i> in Guangdong province, China. The new species is characterised by having a nearly spherical female, with dimensions of length × width = 532.3 (423.8-675.3) × 295.6 (160.0-381.2) μm, stylet length of 35.7 (31.1-42.1) μm, protruding vulval lips, a vulval slit measuring 54.2 (47.4-58.9) μm, an area between the vulva and anus that is flat to concave, and a vulva-anus distance 49.3 (41.1-57.6) μm. The male features two lip annules, a stylet length of 31.7 (27.4-34.8) μm and basal knobs that are slightly projecting anteriorly, while lateral field is areolated with three incisures and spicules length of 27.1 (23.7-31.0) μm. The second stage juvenile is characterised by a body length of 506.1 (441.8-564.4) μm long, two to three lip annules, a stylet length 31.2 (29.7-33.2) μm which is well developed, basal knobs projecting anteriorly, a lateral field that is areolate with three incisures, and a narrow rounded tail measuring 63.2 (54.2-71.3) μm long, with a hyaline region of 35.6 (27.4-56.6) μm long that is longer than the stylet. Based on morphology and morphometrics, the new species is closely related to <i>C. sinensis</i> and <i>C. japonicum</i> within the genus <i>Cryphodera.</i> The phylogenetic trees constructed based on the ITS-rRNA, 28S-rRNA D2-D3 region, and the partial <i>COI</i> gene sequences indicate that the new species clusters with other <i>Cryphodera</i> species but maintains in a separated subgroup. A key to the species of the genus <i>Cryphodera</i> is also provided in this study.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e85"},"PeriodicalIF":1.3,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144707753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metacercariae of <i>Diplostomum</i> spp. from eyes of the Bullhead <i>Cottus koshewnikowi</i> Gratzianov, 1907 from the northern European watershed.","authors":"D Lebedeva, S V Bugmyrin, D Zaitsev, E P Ieshko","doi":"10.1017/S0022149X25100515","DOIUrl":"https://doi.org/10.1017/S0022149X25100515","url":null,"abstract":"<p><p>Metacercariae of <i>Diplostomum</i> spp. are widespread fish parasites. In this study we obtained the first data on infection of Bullhead <i>Cottus koshewnikowi</i> with these larvae in five rivers of northern Europe (Finland and Russia) using molecular and morphological description. Three <i>Diplostomum</i> spp. were revealed in the eyes of bullheads. <i>Diplostomum spathaceum</i> and <i>D. mergi</i> Lineage 3 sensu Georgieva et al. (2013) were found in the lens, while <i>Diplostomum</i> sp. Lineage 6 sensu Blasco-Costa et al. (2014) was found in the retina. We obtained molecular data on these three species and provided morphological characteristics of the latter two species. Partial sequences of the cytochrome c oxidase subunit 1 (<i>cox1</i>) and ITS1-5.8S-ITS2 were amplified for 20 isolates. Using molecular data, we ascertained the species identification and obtained new information on the life cycles of <i>D. mergi</i> Lineage 3 and <i>Diplostomum</i> sp. Lineage 6. Partial <i>cox1</i> sequences were used to assess the haplotype diversity of <i>D. mergi</i> Lineage 3 and <i>Diplostomum</i> sp. Lineage 6 in the study area. Discriminant analysis showed that <i>D. mergi</i> Lineage 3 was morphometrically close both to the species of the lens complex (<i>D. mergi</i> Lineage 2, <i>D. mergi, D. nordmanni</i>, and <i>D. parviventosum</i>) and to the species from the retina (<i>D. pungiti, D. volvens</i>). Dimensions of <i>Diplostomum gobiorum</i> lay far outside the confidence interval of <i>D. mergi</i> Lineage 3. Our molecular and morphological data and the new information about the hosts and the distribution of these parasites are a crucial step towards elucidating the diversity and life cycles of these important parasites. The data on the infection of bullheads in the River Utsjoki (a tributary of the River Teno, Finland) with metacercariae of <i>Diplostomum</i> spp. offer some insights into the relationships between the introduced host and the native parasites. Infection of bullheads, which are considered invasive in the Teno River system, with metacercariae of <i>Diplostomum</i> spp. may lead to increased infection levels in resident fish.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e81"},"PeriodicalIF":1.3,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Steinernema tarimense</i> n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Tarim Basin, Xinjiang, China.","authors":"F Zhan, C Tian, H Li, R Yang, H Bao, S Zhang, X Zhang, Y Shi, M Tomalak, V Půža, W Guo","doi":"10.1017/S0022149X25100448","DOIUrl":"https://doi.org/10.1017/S0022149X25100448","url":null,"abstract":"<p><p>A novel entomopathogenic nematode (EPN) species, <i>Steinernema tarimense</i> n. sp., was isolated from soil samples collected in a <i>Populus euphratica</i> forest located in Yuli County within the Tarim Basin of Xinjiang, China. Integrated morphological and molecular analyses consistently place <i>S. tarimense</i> n. sp. within the '<i>kushidai-</i>clade'. The infective juvenile (IJ) of new species is characterized by a body length of 674-1010 μm, excretory pore located 53-80 μm from anterior end, nerve ring positioned 85-131 μm from anterior end, pharynx base situated 111-162 μm from anterior end, a tail length of 41-56 μm, and the ratios D% = 42.0-66.6, E% = 116.2-184.4, and H% = 25.5-45.1. The first-generation male of the new species is characterized by a curved spicule length of 61-89 μm, gubernaculum length of 41-58 μm, and ratios D% = 36.8-66.2, SW% = 117.0-206.1, and GS% = 54.8-82.0. Additionally, the tail of first-generation female is conoid with a minute mucron. Phylogenetic analyses of ITS, 28S, and <i>mt12S</i> sequences demonstrated that the three isolates of <i>S. tarimense</i> n. sp. are conspecific and form a sister clade to members of the '<i>kushidai-</i>clade' including <i>S. akhursti</i>, <i>S. anantnagense</i>, <i>S. kushidai</i>, and <i>S. populi.</i> Notably, the IJs of the new species exhibited faster development at 25°C compared to other <i>Steinernema</i> species. This represents the first described of an indigenous EPN species from Xinjiang, suggesting its potential as a novel biocontrol agent against local pests.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e80"},"PeriodicalIF":1.3,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological and molecular evaluation of hydatid cysts according to necropsy findings of 52 equids in Türkiye.","authors":"F Celik, B Karabulut, M Uslug, E Cankaya, Y Eroksuz, S Simsek","doi":"10.1017/S0022149X25100552","DOIUrl":"https://doi.org/10.1017/S0022149X25100552","url":null,"abstract":"<p><p><i>Echinococcus equinus</i> is a parasitic cestode primarily maintained within an equine-canine life cycle, with horses, donkeys, mules, and other ungulates serving as intermediate hosts. Although <i>E. equinus</i> has historically been considered non-zoonotic, recent molecular studies suggest that this assumption may need to be reevaluated. This study aimed to investigate the presence and molecular characterization of <i>E. equinus</i> in equids from Türkiye. A retrospective analysis of 52 equine necropsies performed between 2020 and 2025 identified hydatid cysts in one Arabian horse and two donkeys. Gross and histopathological examination confirmed the presence of hydatid cysts in the liver and lungs, exhibiting characteristic structural features. Molecular identification was conducted through PCR amplification targeting the mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) gene, with all positive samples confirmed as <i>E. equinus</i> through sequence analysis. Phylogenetic analysis demonstrated a close relationship between the obtained sequences and reference <i>E. equinus</i> strains from other geographic regions. These findings provide the molecular confirmation of <i>E. equinus</i> in equids from Türkiye and underscore the need for targeted surveillance to better understand its distribution, transmission, and zoonotic relevance, especially considering the first confirmed human case reported in the country in 2021.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e82"},"PeriodicalIF":1.3,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Life cycles of trematodes infecting six species of intertidal gastropods in Japan.","authors":"E Ansai, H Sekine, K Munakata, T Sase, M Sasaki, M Nitta, S Suzuki, T Abe, T Takano, H Fukumori, Y Nakatsubata, M Suzuki, T Waki","doi":"10.1017/S0022149X25100485","DOIUrl":"https://doi.org/10.1017/S0022149X25100485","url":null,"abstract":"<p><p>A variety of larvae and parthenitae of trematodes have been detected in gastropods in the intertidal zone in Japan. However, because of the difficulty associated with the morphological identification of these stages, they have rarely been identified to the species or higher taxonomic levels. In this study, trematodes of these stages were sampled from intertidal gastropods in the Japanese coastal regions and were identified to the species, genus, or family levels morphologically and molecularly to elucidate or predict their life cycles. Investigation of 17 gastropod species (682 individuals in total) from 14 localities led to the detection of trematodes in 47 individuals belonging to six snail species. The infected gastropods were morphologically identified as <i>Nipponacmea fuscoviridis</i>, <i>Monodonta confusa</i>, <i>Trochus sacellum</i>, <i>Batillaria attramentaria</i>, <i>Littorina brevicula</i>, and <i>Purpuradusta gracilis.</i> Our molecular analyses revealed that sporocysts, rediae, and metacercariae from the gastropods were divided into 14 species belonging to nine families: Philophthalmidae, Fellodistomidae, Gymnophallidae, Lepocreadiidae, Heterophyidae, Opisthorchiidae, Notocotylidae, Microphallidae, and Opecoelidae. These trematodes were thought to use fishes, octopuses, seabirds, and marine mammals as their definitive hosts. Marine organisms such as jellyfishes, crustaceans, and fishes are also thought to act as the second intermediate and paratenic hosts of few present trematode species. As for the other trematode species, DNA barcodes of trematodes from various marine organisms will also illuminate the life cycles in future.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e83"},"PeriodicalIF":1.3,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular evidence for phylogenetic affinities of <i>Rhabdochona coronacauda</i> (Thelazioidea: Rhabdochonidae) with other spiruromorph nematodes.","authors":"K S Vainutis, A N Voronova, V P Belyaev, N E Zyumchenko, M E Shapovalov","doi":"10.1017/S0022149X25100588","DOIUrl":"https://doi.org/10.1017/S0022149X25100588","url":null,"abstract":"<p><p>The nematodes isolated from three species of Chanodichthys were completely consistent with the morphological description of Rhabdochona coronacauda. We provide new morphometric data for R. coronacauda, which slightly differ from those of the original description. For the first time, we performed a phylogenetic analysis by using both primary (linear) and secondary (folded structures) sequences of the complete 18S rRNA gene for three superfamilies of Spiruromorpha. The interspecific genetic distances within the genus Rhabdochona were 0.13%-3.06% between 18 species. Rhabdochona coronacauda was sister to a sub-group consisting of the type species R. denudata, R. hospeti, R. hellichi, and R. turkestanica. The secondary structures reconstructed for 35 species of three superfamilies from the order Spiruromorpha comprised 16 conformations of the region including helix 39 and expansion segment 9, and two conformations of helix 17. Helix 39 can be used to differentiate single species or separate species groups of Rhabdochona. The structures of helix 39 in both Rhabdochonidae and Cystidicolidae with Salmonema had an identical 39a domain but differed in the 39b domain and expansion segment 9. In addition, the structure of the helix 39-expansion segment 9 domain within and between families of different superfamilies and their resolution on the phylogenetic tree in combination did not correspond to the accepted classification of spiruromorph nematodes. Helix 17 did not differ within Rhabdochona, or between Rhabdochonidae and Cystidicolidae. At the superfamily level, helix 17 can distinguish Thelazioidea, Spiruroidea, and Habronematoidea from Rhabdochonidae, and Cystidicolidae from Spirocercidae, Thelaziidae, and Pneumospiruridae.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e84"},"PeriodicalIF":1.3,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic variation and population structure of <i>Haemonchus contortus</i>: an <i>in-silico</i> analysis.","authors":"W Wei, Z Lan, Xuewei Liu, Xinhui Zhang, X Gu, R Wang","doi":"10.1017/S0022149X25100357","DOIUrl":"https://doi.org/10.1017/S0022149X25100357","url":null,"abstract":"<p><p><i>Haemonchus contortus</i> is a parasitic nematode that causes significant economic losses in ruminant livestock worldwide. In this study, we assessed the global genetic diversity and population structure of <i>H. contortus</i> using mitochondrial <i>COX1</i> and ribosomal <i>ITS2</i> sequences retrieved from the NCBI GenBank database. In total, 324 haplotypes of the <i>COX1</i> and 72 haplotypes of the <i>ITS2</i> were identified. The haplotype diversity values were all higher than 0.5, and the nucleotide diversity values were higher than 0.005. The Tajima's D value for <i>COX1</i> (-1.65634) was higher than that for <i>ITS2</i> (-2.60400). Fu's Fs, Fu and Li's D (FLD), and Fu and Li's F (FLF) values also showed high negative values, indicating a high probability of future population growth. In addition, the high fixation index (FST) value suggests significant genetic differentiation among populations. The haplotype networks of <i>H. contortus</i> populations based on <i>COX1</i> sequences revealed clear geographic clustering, whereas <i>ITS2</i> sequences showed more haplotype admixture across regions. The results of phylogenetic analyses were consistent with the haplotype networks. These findings highlighted that <i>H. contortus</i> populations exhibit significant genetic variation and are undergoing rapid population expansion, with clear genetic differences across geographic regions. This study established critical baseline data for future molecular epidemiology studies, which could guide region-specific parasite surveillance and targeted control strategies, thus helping to mitigate the risk of cross-border parasite transmission and drug resistance.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e78"},"PeriodicalIF":1.3,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trematode diversity in southern Africa: metacercariae of the Clinostomidae and Cryptogonimidae in intermediate freshwater fish hosts.","authors":"M Truter, R Q-Y Yong, W Smit, A Chakona, W J Luus-Powell, N J Smit","doi":"10.1017/S0022149X2510045X","DOIUrl":"https://doi.org/10.1017/S0022149X2510045X","url":null,"abstract":"<p><p>The freshwater fish fauna of southern Africa is highly diverse; however, the magnitude of parasitic species they host is unevenly known. The region's documented adult trematode fish fauna is sparse, while the opposite is evident for intermediate trematode stages. Perceived difficulty in identification of underdeveloped stages lead to the exclusion of reporting metacercariae or lack either morphological or molecular data resulting in a depauperate comparative molecular data repository for species of the region and Africa as a whole. In an effort to address the morphological and molecular data void of the parasite fauna of southern African freshwater fishes, we sought to comprehensively investigate and characterise this fauna. Here we report on three metacercarial forms of <i>Clinostomum</i> (Clinostomidae) from three fish families (Clariidae, Mochokidae, and Mormyridae), provide the first report of a species of the Cryptogonimidae from a cyprinid host in South Africa, and include molecular data for the partial 28S rDNA, ITS1-2 and COI mtDNA regions of these metacercarial forms. Our clinostomid specimens morphologically and genetically corresponded with <i>Clinostomum brieni</i> (e.g., <i>Clarias gariepinus</i>) and <i>Clinostomum</i> 'morphotype 2' and 'morphotype 3' per Caffara <i>et al.</i> (2017) from the mormyrid <i>Marcusenius pongolensis</i> and the mochokid catfish <i>Chiloglanis</i> sp., respectively. Our cryptogonimid metacercariae did not correspond with any known species or available molecular sequence data; however, the presence of robust circumoral spines on the oral sucker indicated that they are either a species of <i>Acanthostomum</i> or <i>Proctocaecum.</i> The molecular data we provide are the first for an <i>Acanthostomum</i>/<i>Proctocaecum</i>-type cryptogonimid from Africa.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e75"},"PeriodicalIF":1.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fasciolosis in Algerian livestock: Epidemiological trends and economic consequences.","authors":"C Fennouh, D Mokrani, H Dahmani, O Salhi, I Ouchetati, M Nabi, K Aouina, I Chettih, N Ouchene, N A Khelifi Touhami","doi":"10.1017/S0022149X25100503","DOIUrl":"https://doi.org/10.1017/S0022149X25100503","url":null,"abstract":"<p><p>Fasciolosis, a parasitic disease of ruminants, poses significant economic and animal-health challenges in Algeria. This study aimed to assess spatial, temporal, and species-specific patterns of fasciolosis prevalence across diverse agro-climatic zones and to estimate associated economic losses. Between 2013 and 2023, eight wilayas El-Tarf, Skikda, Jijel (Region I), Blida, Mila (Region II), and M'Sila, Medea, Laghouat (Region III) were surveyed. Systematic postmortem inspections of 1,569,392 animals (349,176 cattle; 982,669 sheep; 235,639 goats; 1,882 camels; 26 horses) were performed by qualified veterinarians, with liver and bile-duct examination for <i>Fasciola.</i> Data on region, species, year, and season were analyzed in R 4.4.0 using ANOVA, Kruskal-Wallis, Tukey's post hoc tests, and principal component analysis (PCA). Economic losses were calculated from condemned liver weights in Blida, Laghouat, and Jijel, converted to USD. PCA distinguished three regional prevalence profiles, with PC1 (77.7% variance) separating overall prevalence levels. Region I exhibited the highest mean prevalence (2.47%), peaking at 3.54% in 2018 - significantly greater than Region II (1.39%) and Region III (1.96%) (p < 0.01). Cattle showed the greatest infection rate (mean 4.14%), significantly higher than sheep (1.32%; p < 0.001) and goats (0.25%; p < 0.001), while horses and camels remained uninfected. Seasonal analysis revealed highest prevalence in autumn and winter (≈2.1%) versus spring (≈1.5%). Economic losses totaled USD 10.6 million in Blida, USD 1.0 million in Laghouat, and USD 142.2 million in Jijel over the study period. Targeted control strategies, adapted to regional and seasonal risk patterns, are essential. Future work should investigate environmental and management factors driving regional differences and evaluate cost-effective interventions to mitigate fasciolosis impact in Algerian livestock.</p>","PeriodicalId":15928,"journal":{"name":"Journal of Helminthology","volume":"99 ","pages":"e77"},"PeriodicalIF":1.3,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}