Journal of food protection最新文献

{"title":"Food Safety Knowledge, Attitudes, and Practices among Vegetable Handlers in Bangladesh","authors":"Ismat Ara Begum ,&nbsp;Mohammad Jahangir Alam ,&nbsp;Bhavani Shankar ,&nbsp;Tamanna Mastura ,&nbsp;Gregory Cooper ,&nbsp;Karl Rich ,&nbsp;Panam Parikh ,&nbsp;Nazmun N. Ratna ,&nbsp;Suneetha Kadiyala","doi":"10.1016/j.jfp.2024.100428","DOIUrl":"10.1016/j.jfp.2024.100428","url":null,"abstract":"<div><div>This study delves into the Knowledge, Attitudes, and Practices of vegetable handlers in Jashore Sadar, Bangladesh, specifically focusing on the crucial significance of food safety across the entire farm-to-fork process. Integrating quantitative and qualitative data provides a multifaceted depiction of this pivotal component within the food supply chain. Ten focus group discussions were conducted, and in total, 50 vegetable handlers were interviewed separately. In the survey, there were 17 questions on food safety knowledge, 16 on food safety attitudes, and 17 on food safety-related practices followed by vegetable handlers. We found that while approximately 60% of handlers had good knowledge about food safety, only about one-quarter of the interviewed vegetable handlers had good attitudes and practices toward food safety. The attitudes and practices of individuals involved in handling vegetables demonstrate a complex range of variations shaped by factors such as educational background, prior experience, and availability of food safety training. Concerns regarding the rising expenses linked to adherence to food safety regulations and a perceived lack of accountability among those involved were also noted. The interviewed wholesaler group reported that approximately BDT 100–150 (USD 0.91–1.37¹)/maund<span><span>¹</span></span> was incurred in maintaining proper safety practices, while the interviewed retailer group said costs would increase by BDT 20–40 (USD 0.18–0.36)/maund if they followed proper handling practices. It is important to note that vegetable handlers recognize the favorable economic consequences of implementing appropriate food safety measures. This study highlights the significance of implementing focused educational and policy interventions to address knowledge disparities and encourage the adoption of food safety procedures among individuals involved in handling vegetables.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 1","pages":"Article 100428"},"PeriodicalIF":2.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11718158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Apple Detection Method Based on Multidimensional Feature Extraction Network Model and Transformer Module","authors":"Wei Ji,&nbsp;Kelong Zhai,&nbsp;Bo Xu,&nbsp;Jiawen Wu","doi":"10.1016/j.jfp.2024.100397","DOIUrl":"10.1016/j.jfp.2024.100397","url":null,"abstract":"<div><div>To enhance the fast and accurate detection of pollution-free green apples for food safety, this paper uses the DETR network as a framework to propose a new method for pollution-free green apple detection based on a multidimensional feature extraction network and Transformer module. Firstly, an improved DETR network main feature extraction module adopts the ResNet18 network and replaces some residual layers with deformable convolutions (DCNv2), enabling the model to better adapt to pollution-free fruit changes at different scales and angles, while eliminating the impact of microbial contamination on fruit testing; Subsequently, the extended spatial pyramid pooling model (DSPP) and multiscale residual aggregation module (FRAM) are integrated, which help reduce feature noise and minimize the loss of underlying features during the feature extraction process. The fusion of the two modules enhances the model’s ability to detect objects of different scales, thereby improving the accuracy of near-color fruit detection. At the same time, in order to solve the problems of slow convergence speed and large calculation amount of the basic network model, the convergence speed of the overall network model is improved by replacing the attention mechanism of Transformer. Experimental results show that compared with the original DETR model, the proposed algorithm has improved in AP, AP50, and AP75 indicators, especially in the AP50 indicator, which has the most obvious improvement reaching a detection accuracy of 97.12%. In the meantime, the trained network model is deployed on the picking robot. Compared with the original DETR network model, its average detection accuracy is as high as 96.58%, and the detection speed is increased by about 51%. Mixed sample detection tests were carried out before and after the model deployment, and the detection rate of the proposed method for nonpolluted fruits reached more than 0.95. enabling the picking robot to efficiently complete the task of picking green apples. The test results show that the algorithm proposed in this article exhibits great potential in the task of detecting pollution-free near-color fruits by the picking robot. It ensures pollution-free fruit picking and the application of AI in food safety.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 1","pages":"Article 100397"},"PeriodicalIF":2.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142590721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of National Chemical Hazards Monitoring, Total Diet Study, and Human Biomonitoring Programmes for Food Safety Exposure Assessment in Singapore","authors":"Angela Li ,&nbsp;Jun Cheng Er ,&nbsp;Wei Ching Khor ,&nbsp;Mei Hui Liu ,&nbsp;Valerie Sin ,&nbsp;Sheot Harn Chan ,&nbsp;Kyaw Thu Aung","doi":"10.1016/j.jfp.2024.100414","DOIUrl":"10.1016/j.jfp.2024.100414","url":null,"abstract":"<div><div>Food safety and food security can impact the quality of human life, and these two aspects are interrelated alongside many influencing external factors. Global stressors such as climate change, recent pandemic, and geopolitical tensions have demonstrated tangible impacts on food security and safety. Food and food system innovation is a key strategy towards feeding the world in a more sustainable and climate-resilient manner. This paper highlights the use of a science-based risk assessment and management in Singapore’s food safety system, specifically in the integration of exposure assessment approaches to support evidence-based food safety risk analysis and decision-making. The use of complementary top-down and bottom-up exposure assessment approaches through the market monitoring programme, total diet study and human biomonitoring forms a comprehensive integrated exposure assessment strategy which can ultimately inform policy and measures in ensuring and securing a supply of safe food. The discussion on such application for chemical food safety in Singapore offers additional insights into the synergistic inter-relationships contributing to the exposure assessment associated with chemicals in food.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 1","pages":"Article 100414"},"PeriodicalIF":2.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Improved Sanitation Standard Operating Procedures on Microbial Populations at Three Tree Fruit Packing Facilities","authors":"M. Laura Rolon ,&nbsp;Marysabel Mendez Acevedo ,&nbsp;Priscilla Sinclair ,&nbsp;Dumitru Macarisin ,&nbsp;Luke F. LaBorde ,&nbsp;Jasna Kovac","doi":"10.1016/j.jfp.2024.100436","DOIUrl":"10.1016/j.jfp.2024.100436","url":null,"abstract":"<div><div>Cleaning and sanitizing are of vital importance to control <em>Listeria monocytogenes</em> in food processing facilities. Here, we evaluated the effect of four cleaning and sanitation standard operating procedures (SSOPs; T1, T2, T3, T4) on the reduction of total aerobic mesophilic microorganisms, the occurrence of <em>L. monocytogenes,</em> and the microbiota composition in three tree fruit packing facilities (F1, F2, and F3) over two packing seasons (Y1 and Y2). Environmental samples were collected from non-food contact surfaces before and after the application of SSOPs. Total aerobic bacteria were quantified using a standard plate count method, and <em>Listeria</em> spp. and <em>L. monocytogenes</em> concentration was quantified using a Most Probable Number method. Amplicon sequencing was used to determine bacterial and fungal microbiota composition, and Nanopore sequencing was used to detect functional elements in the microbiota that could promote the survival and persistence of <em>L. monocytogenes</em> in the studied environments. The use of SSOPs reduced the total bacterial load by 0.27–2.48 log₁₀ CFU/swab (<em>p</em> ≤ 0.001). Among the treatments tested, the inclusion of a biofilm remover in T4 was most effective in significantly reducing the total <em>Listeria</em> spp. concentration by 1.57–1.27 log₁₀ MPN/swab (<em>p</em> &lt; 0.02) and the frequency of <em>L. monocytogenes,</em> although the latter was not statistically significant. We observed inconsistent changes in the bacterial and fungal microbiota composition due to the application of cleaning and sanitizing SSOPs, which may be due to the presence of dead DNA after the treatment. Using Nanopore sequencing, we detected functional elements related to biofilm formation and stress resistance in the microbiomes of the studied environments. Overall, our study shows that the implementation of SSOPs improved the sanitation outcomes in tree fruit packing facilities. There is a need for the future work to focus on optimizing and validating the standard operating procedures, especially in the areas in which SSOPs were less effective, such as those covered with wax residues.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 1","pages":"Article 100436"},"PeriodicalIF":2.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contamination of Japanese Retail Foods With Enterotoxigenic Clostridium Perfringens Spores","authors":"Takahiro Ohnishi ,&nbsp;Maiko Watanabe ,&nbsp;Yusuke Yodotani ,&nbsp;Emiri Nishizato ,&nbsp;Seiya Araki ,&nbsp;Satomi Sasaki ,&nbsp;Yukiko Hara-Kudo ,&nbsp;Yuka Kojima ,&nbsp;Naoaki Misawa ,&nbsp;Nobuhiko Okabe","doi":"10.1016/j.jfp.2024.100429","DOIUrl":"10.1016/j.jfp.2024.100429","url":null,"abstract":"<div><div>The contamination of Japanese retail foods and the intestinal contents of animals with the spores of enterotoxigenic <em>Clostridium perfringens</em> were investigated by analyzing clostridial toxin genes (<em>cpa</em> and <em>cpe</em>) using a culture method and PCR. Enterotoxigenic <em>C. perfringens</em> was detected in 12.3% (8/65 samples) of shellfishes, 8.4% (7/83 samples) of dried seafoods, 7.4% (15/204 samples) of curry mixes and spices, 2.6% (1/39 samples) of dried seaweeds, 2.5% (2/79 samples) of fishes and shrimp, 1.9% (2/105 samples) of chicken, and 0.8% (1/121 samples) of root vegetables. Enterotoxigenic <em>C. perfringens</em> was not detected in beef (95 samples) and pork (110 samples). The ratio of enterotoxigenic <em>C. perfringens</em>-positive to all <em>C. perfringens</em>-positive samples was high for fish and shrimp (40.0%), curry mixes and spices (19.0%), shellfish (18.1%), dried seafood (16.7%), and dried seaweed (16.7%). Although <em>C. perfringens</em> was investigated in the intestinal contents of cattle (212 samples), pigs (207 samples), and chicken (159 samples), enterotoxigenic <em>C. perfringens</em> was not detected. These results indicate that beef and pork sold in Japan are unlikely to be contaminated with enterotoxigenic <em>C. perfringens</em>, and that other foods such as curry powder, shellfish, and dried seafoods are more important as the sources of contamination in Japan. Dried seafoods are frequently used to make soup stock in Japanese and other Asian dishes. In cases of food-borne illness linked to <em>C. perfringens</em> contamination of Japanese and Asian dishes, dried seafood should be investigated, in addition to other ingredients such as meat.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 1","pages":"Article 100429"},"PeriodicalIF":2.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survival of Generic Escherichia coli on In-Field Mature and Immature Gala and Golden Delicious Apples With or Without Overhead Evaporative Cooling Treatment","authors":"Claire M Murphy ,&nbsp;Kyu Ho Jeong ,&nbsp;Lauren Walter ,&nbsp;Manoella Mendoza ,&nbsp;Tonia Green ,&nbsp;Andy Liao ,&nbsp;Karen Killinger ,&nbsp;Ines Hanrahan ,&nbsp;Mei-Jun Zhu","doi":"10.1016/j.jfp.2024.100410","DOIUrl":"10.1016/j.jfp.2024.100410","url":null,"abstract":"<div><div>The application of overhead evaporative cooling (EC) with untreated surface water is common in Washington State to decrease sunburn on apples. While this practice reduces crop and economic losses, applying untreated surface water to produce introduces potential risks of foodborne pathogen contamination. This study examined EC and the survival of inoculated generi<em>c Escherichia coli</em> for two apple varieties over three growing seasons. Factors examined included EC treatment (conventional, untreated), canopy location (high, low), and apple variety (Gala, Golden Delicious). Fruit maturity (mature, immature) was also examined for one year. A rifampicin-resistant generic <em>E. coli</em> cocktail was applied to apples at 7.3 ± 0.4 log CFU/apple, and apples were enumerated for <em>E. coli</em> levels at 0, 2, 10, 18, 34, 42, 58, 82, 106, and 154 h postinoculation. Log-linear, Weibull, and Biphasic models were utilized to characterize the die-off pattern of <em>E. coli</em>. For most treatments, inoculated <em>E. coli</em> decreased by almost or over 6 log CFU/apple over the study duration; however, <em>E. coli</em> remained detectable at low levels on apples at 154 h postinoculation. EC treatment and canopy location did not significantly impact daily linear <em>E. coli</em> die-off rates. Apple variety and maturity demonstrated small but statistically significant impacts on daily linear die-off rates. Nonlinear models (i.e., biphasic and Weibull) best captured <em>E. coli</em> die-off on apples, showing a rapid initial decrease followed by a slower decline over time. Overall, results demonstrate that EC, a useful fruit quality practice, did not impact the survival of inoculated generic <em>E. coli</em> on apple surfaces. The study provides valuable insights for the apple industry regarding <em>E. coli</em> die-off rates on in-field apples, guiding practical decisions to mitigate risk.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 1","pages":"Article 100410"},"PeriodicalIF":2.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Food Safety Emergency Incidents on Sina Weibo: Using Text Mining and Sentiment Evolution","authors":"Biao Ma ,&nbsp;Ruihan Zheng","doi":"10.1016/j.jfp.2024.100418","DOIUrl":"10.1016/j.jfp.2024.100418","url":null,"abstract":"<div><div>Food safety remains a crucial concern in both public health and societal stability. In the age of information technology, social media has emerged as a pivotal channel for shaping public opinion and disseminating information, exerting a substantial influence on how the public perceives incidents related to food safety. This study specifically focuses on the “Rat-Headed Duck Neck” incident as a case study, conducting a comprehensive analysis of extensive social media data to investigate how online public discourse molds perceptions of such events. To accomplish this research, data were initially gathered using a custom web crawler technology. These data encompassed various aspects, including user interactions, emotional expressions, and the evolution of topics. Subsequently, the study employed an innovative approach by combining BERT-TextCNN and BERTopic models for a thorough analysis of sentiment and thematic aspects of the textual data. This analysis provided insights into the intricate emotions and primary concerns of the public regarding incidents related to food safety. Furthermore, the research harnessed Gephi, a network analysis tool, to scrutinize the dissemination of information within the network and to monitor dynamic shifts in public opinion. The findings from this study not only shed light on the role of online public sentiment in the propagation of food safety events but also provide fresh perspectives for policymakers and business leaders when responding to similar crises, taking into account the subtleties of online public sentiment. These innovative methodologies and findings significantly enhance our comprehension of public responses to food safety incidents within the realm of social media.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 1","pages":"Article 100418"},"PeriodicalIF":2.1,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Outbreak Data Reveals Factors Contributing to Salmonellosis Outbreaks Linked to Cantaloupes","authors":"Megan Rose-Martel ,&nbsp;Sandeep Tamber","doi":"10.1016/j.jfp.2024.100404","DOIUrl":"10.1016/j.jfp.2024.100404","url":null,"abstract":"<div><div>Over the last thirty years, the presence of <em>Salmonella</em> spp. on cantaloupes has been linked to multiple large and deadly foodborne outbreaks in multiple countries. To identify factors associated with the number of cases and risk of severe illness in these outbreaks, information from previous melon-associated salmonellosis outbreaks was analyzed. Data were collected from sixty outbreak investigations. Compared to other melon types, such as watermelon, honeydew, and Galia melon, cantaloupes had the highest public health burden. Cantaloupes were implicated in 43% of reported melon-related outbreaks, 51% of melon-related laboratory-confirmed cases, 54% of melon-related hospitalizations, and 76% of melon-related deaths. In the United States, imported cantaloupes were associated with higher rates of severe salmonellosis and a greater diversity of <em>Salmonella</em> spp. serovars compared to domestically grown cantaloupes. Cantaloupes implicated in outbreaks were equally likely to have been consumed in either private or public settings. Larger outbreaks were associated with the consumption of precut cantaloupe and/or the consumption of cantaloupes in public settings. With the identification of these contributing factors, a literature search was conducted to assess the state of knowledge concerning <em>Salmonella</em> and cantaloupes. Several gaps in the literature were noted and are discussed in the context of reducing the number of illnesses associated with the presence of <em>Salmonella</em> on cantaloupes.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 1","pages":"Article 100404"},"PeriodicalIF":2.1,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Storage Conditions on Salmonella enterica and Listeria monocytogenes in Pre- and Post-Printed 3D Food Ink","authors":"Allyson N. Hamilton,&nbsp;Kristen E. Gibson","doi":"10.1016/j.jfp.2024.100409","DOIUrl":"10.1016/j.jfp.2024.100409","url":null,"abstract":"<div><div>3D food printers (3DFPs) allow for the customization of physicochemical properties of foods in new ways. Storage conditions for food ink capsules and printed food inks have not been investigated. This study aimed to determine the impact of storage temperature, time, and method (pre- vs. postprinting) on <em>Salmonella enterica</em> and <em>Listeria monocytogenes</em>. A bacterial cocktail was cultured in minimal media and added to a protein cookie food ink at ∼6.5 log CFU/g. The inoculated food ink was divided into 18 capsules (50 g/capsule); half were 3D printed. The remaining capsules and printed products were stored at three temperatures [20 °C, 4 °C, −18 °C]. Selective media (XLT-4 and CHROMagar Listeria) were used for pathogen enumeration. Aerobic plate count and yeast counts were performed at each time point. The pH and water activity (<em>a_w</em>) of the food ink were measured at the initial and final timepoints. A significant four-way interaction effect was observed between microorganism type (<em>L. monocytogenes/Salmonella</em>), time, temperature, and storage method (capsule/print) (<em>p</em> = 0.014). Significant findings include (1) at −18 °C, concentrations of <em>L. monocytogenes</em> decreased between Day 0 and Day 1, (2) at 20 °C, concentrations of <em>S. enterica</em> were significantly higher in the capsule than in the printed food on Day 1 (<em>p</em> &lt; 0.0001), and (3) at 4 °C, concentrations of <em>S. enterica</em> were significantly higher in the printed food on Day 5 compared to Day 1 (<em>p</em> &lt; 0.0001) with a 0.9 (95% CI: 0.89, 0.91) log increase. In addition, a significant three-way interaction effect was found between microorganism type (yeast/aerobic counts), time, and temperature (<em>p</em> = 0.024). Yeast counts remained steady at all temperatures, while aerobic counts increased at 4 °C. Minimal differences were observed between <em>Listeria</em> and <em>Salmonella</em> and their responses to varying storage conditions over time indicating that storage method and temperature may be less important for a low-water activity product such as protein cookie food ink.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 1","pages":"Article 100409"},"PeriodicalIF":2.1,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the Antibacterial, Antioxidant, and Cholesterol-lowering Properties of Yogurt Fortified with Postbiotic of Lactobacillus acidophilus and Lactiplantibacillus plantarum in the Wistar Rat Model","authors":"Sareh Davarzani ,&nbsp;Mohammad Reza Sanjabi ,&nbsp;Naheed Mojgani ,&nbsp;Saeed Mirdamadi ,&nbsp;Mostafa Soltani","doi":"10.1016/j.jfp.2024.100408","DOIUrl":"10.1016/j.jfp.2024.100408","url":null,"abstract":"<div><div>Postbiotics have gained attention in the food industry due to their functional properties and ease of use compared to their live parent cells. Postbiotics are the metabolic byproducts of probiotic microorganisms, offering advantages such as antimicrobial and anti-diabetic effects. The study aimed to explore the potential antibacterial, antioxidant, and cholesterol-lowering effects of postbiotics from <em>Lactobacillus acidophilus</em> (<em>LA</em>) and <em>Lactiplantibacillus plantarum</em> (<em>LbP</em>) through <em>in vitro</em> and <em>in vivo</em> studies. Freeze-dried postbiotics from <em>L. acidophilus</em> BLAC 258 and <em>L. plantarum</em> were used in yogurt to inhibit foodborne pathogens over a 21-day storage period at 4 °C. The cholesterol-lowering effects of the postbiotic yogurt were assessed in Wistar rats fed with Normal Basal Diet (NBD) and High Cholesterol Diet (HCD). All experiments were performed in triplicate, and the collected data were analyzed with a one-way ANOVA using SPSS v.20 (2021) software. The Tukey Honestly Significant Difference (HSD) test was used for means differences at the 95% confidence interval. The results showed that postbiotic-fortified yogurt exhibited significant antioxidant and antibacterial effects. The antioxidant capacity of the yogurt increasingly peaked at 48.81% on day 14. Also, <em>Listeria monocytogenes</em> counts in the postbiotic yogurt decreased by approximately 2 Log₁₀ on day 3. High-cholesterol-fed rats receiving postbiotic yogurt experienced significant reductions in total cholesterol, triglycerides, and LDL levels. Overall results indicate that postbiotics functional yogurt might be a safe and effective strategy for managing cholesterol levels and inhibiting foodborne pathogens.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"87 12","pages":"Article 100408"},"PeriodicalIF":2.1,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}