Journal of Indian Society of Periodontology最新文献

{"title":"\"Efficacy of cytotoxic effect of green tea catechins on the human periodontal fibroblasts and human dental pulp fibroblasts -An in vitro study\".","authors":"Sanjeevini Hattarki,&nbsp;Chetana Bogar,&nbsp;Kishore Bhat","doi":"10.4103/jisp.jisp_168_22","DOIUrl":"10.4103/jisp.jisp_168_22","url":null,"abstract":"<p><strong>Background: </strong>Inflammation of tooth-supporting tissue and the pulp tissue is followed by wound healing and regeneration process that involves the specific type of connective tissue cells, the fibroblasts. During periodontitis and pulpitis, the inflammation of the tissue causes damage to the fibroblasts. These fibroblasts secrete collagen proteins and maintain the structural framework; along with this the inflammatory process moves toward healing where in the specific cells such as the fibroblast cells play important roles. Green tea catechins epigallocatechin-3-gallate (EGCG) being one of the major catechins is known to have multiple beneficial effects on human fibroblasts.</p><p><strong>Objective: </strong>To assess the <i>in vitro</i> cytotoxicity of green tea catechins on the human periodontal ligament (PDL) fibroblasts and human dental pulp fibroblasts.</p><p><strong>Materials and methods: </strong>Human PDL fibroblasts (hPDLFs) and human dental pulp fibroblasts were isolated from the two extracted premolar teeth that were indicated for orthodontic treatment. The fibroblasts were then seeded in 96 well tissue culture plate for cell viability study. EGCG was used at different concentration to treat the cells. After 48 h; (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT) assay was performed to determine the cell viability.</p><p><strong>Results: </strong>The vitality of hPDLFs and human dental pulp fibroblasts was found to be inversely proportional to EGCG concentrations.</p><p><strong>Conclusions: </strong>hPDLFs have shown 37% proliferation at lowest concentration of EGCG used and human dental pulp fibroblasts have shown 99% viability at lowest concentration of EGCG used.</p>","PeriodicalId":15890,"journal":{"name":"Journal of Indian Society of Periodontology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ISP Scientific Events.","authors":"","doi":"10.4103/jisp.jisp_176_23","DOIUrl":"10.4103/jisp.jisp_176_23","url":null,"abstract":"","PeriodicalId":15890,"journal":{"name":"Journal of Indian Society of Periodontology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281309/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9684257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the effects of hydrogen water on human gingival fibroblast cell culture in patients with chronic periodontitis.","authors":"Akanksha Bhatt,&nbsp;Aarati Nayak,&nbsp;Kishore Bhat,&nbsp;Chetana Bogar,&nbsp;Ranganath Nayak,&nbsp;Sachita Naik","doi":"10.4103/jisp.jisp_546_21","DOIUrl":"10.4103/jisp.jisp_546_21","url":null,"abstract":"<p><strong>Background: </strong>Activated inflammatory cells produce reactive oxygen species (ROS) to eliminate pathogens. Under normal conditions, the pathogens are taken care of, and tissues are repaired. However, in periodontal disease, persistent inflammation causes increased ROS release and impaired healing. Therefore, removal of overproduced ROS using antioxidants is necessary. Hydrogen water has an antioxidative effect on cells and impedes oxidative stress-related disorders.</p><p><strong>Aim: </strong>To study the effect of hydrogen water on cell viability, migration, and its antioxidative potential in fibroblasts obtained from chronic periodontitis patients.</p><p><strong>Materials and methods: </strong>The gingival tissue samples were obtained from 26 subjects (13 periodontally healthy individuals and 13 chronic periodontitis patients) and processed. The human gingival fibroblasts were cultured and the assays were commenced once adequate growth was detected. The effect of hydrogen water on cell viability was checked by neutral red assay, while the migration potential was assessed by transwell migration assay. The antioxidative potential of hydrogen water was evaluated by CUPRAC assay.</p><p><strong>Statistical analysis: </strong>Intergroup comparison was done using Mann-Whitney <i>U</i>-test. Intragroup comparison was done using Wilcoxon signed-rank test.</p><p><strong>Results: </strong>Hydrogen water was nontoxic to the fibroblasts at 24 h and 48 h. The intergroup comparison of the cell viability between hydrogen water-treated periodontally healthy gingival fibroblasts (HF) and fibroblasts from patients with chronic periodontitis (CF) showed a statistically significant (<i>P</i> = 0.00) difference at 24 h and 48 h. Hydrogen water also positively influenced the migratory capacity. Hydrogen water-treated fibroblasts obtained from chronic periodontitis patients showed more migration in comparison to the healthy group (<i>P</i> = 0.00). Hydrogen water showed an antioxidative potential. The maximum potential was seen in relation to the fibroblasts obtained from chronic periodontitis patients at 48 h.</p><p><strong>Conclusion: </strong>Hydrogen water was nontoxic, increased the migratory capacity, and showed an antioxidative potential on human fibroblasts obtained from periodontally healthy individuals and patients with chronic periodontitis.</p>","PeriodicalId":15890,"journal":{"name":"Journal of Indian Society of Periodontology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281311/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9709338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effectiveness of green tea mouth rinse on oral halitosis, tongue coating, and plaque accumulation in comparison with 0.2% chlorhexidine mouth rinse - A double-blind randomized control trial.","authors":"Pratibha Sharma,&nbsp;Byalakere Rudraiah Chandrashekar,&nbsp;Kenganura Mruthunjaya,&nbsp;Vivek Bhaskar","doi":"10.4103/jisp.jisp_355_22","DOIUrl":"10.4103/jisp.jisp_355_22","url":null,"abstract":"<p><strong>Background: </strong>Green tea (<i>Camellia sinensis</i>) mouth rinse is found effective in reducing periodontitis. However, studies evaluating the effectiveness of green tea extracts in reducing oral halitosis and tongue coating on Indian population were scanty.</p><p><strong>Objective: </strong>The objective of this study was to evaluate the effectiveness of green tea-based mouth rinse in comparison with 0.2% chlorhexidine gluconate mouth rinse in reducing dental plaque, tongue coating, and halitosis among human volunteers.</p><p><strong>Materials and methods: </strong>This was a parallel-arm double-blind randomized controlled trial conducted in two residential hostels in Mysuru city over 21 days. 90 adult participants were recruited and randomized into three groups: Group A: mouth rinse containing saline, Group B: 5% <i>C. sinensis</i> mouth rinse, and Group C: 0.2% chlorhexidine diluted to with equal quantity of water. Preintervention prophylaxis was done; tongue coating and oral halitosis scores were recorded and compared between the groups at baseline and after 21 days.</p><p><strong>Results: </strong>The mean plaque buildup at postintervention was highest in Group 1 (2.45 ± 0.38) followed by Group 3 (1.18 ± 0.12) and Group 2 (1.08 ± 0.11) in the descending order. The mean oral halitosis score was highest in Group 1 (3.00 ± 0.79) followed by Group 3 (1.53 ± 0.50) and Group 2 (1.50 ± 0.50) in the descending order. The mean tongue coating score was highest in Group 1 (1.17 ± 0.47) followed by Group 2 (0.75 ± 0.36) and Group 3 (0.69 ± 0.34) in the descending order.</p><p><strong>Conclusion: </strong>Five percent <i>C. sinensis</i> mouth rinse is as effective as commercially available 0.2% chlorhexidine mouthwash in reducing plaque deposition, tongue coating, and oral halitosis.</p>","PeriodicalId":15890,"journal":{"name":"Journal of Indian Society of Periodontology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281303/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of vestibular depth: An observational cross-sectional study.","authors":"Tanya Nandkeoliar,&nbsp;Ashish Kumar,&nbsp;Sachidananda Chungkham,&nbsp;Sinam Subhaschandra Singh","doi":"10.4103/jisp.jisp_293_22","DOIUrl":"10.4103/jisp.jisp_293_22","url":null,"abstract":"<p><strong>Background: </strong>The vestibular depth (VD) varies to a great extent among individuals and at different points of reference. The normal range of depth of the vestibule at different areas in the mouth remains to be established.</p><p><strong>Aim: </strong>The primary objective of this study is to assess the average VD around different teeth in the maxilla and the mandible. Furthermore, an attempt has been made to identify the various ranges of VD and understand what could be suggestive of a normal VD.</p><p><strong>Materials and methods: </strong>The participants included were divided into four age groups, i.e., 6-14 years (Group 1), 15-30 years (Group 2), 31-50 years (Group 3), and >50 years (Group 4). Further, the subjects were grouped into subgroup A, periodontally healthy and subgroup B, gingival recession (GR). Group 1, 2, and 3 included 30 subjects each in subgroup A, while Group 4 subgroup B had 18 subjects. In case of subgroup B, Group 2 included 9 subjects, Group 3-31 subjects, and Group 4-30 subjects. Measurements included the depth of vestibule from 2 reference point, i.e., gingival margin (GM) and incisal edge (IE).</p><p><strong>Results: </strong>In subgroup A, the average VD from GM was found to be the least in the Group 1 i.e., 6-14 years (9.2 mm ± 4.26 in maxilla and 7.01 mm ± 3.14 in the mandible) and the highest in the Group 4 i.e., >50 years (12.06 mm ± 2.73 in maxilla and 10.08 mm ± 2.58 in the mandible). In the case of subgroup B, the least depth from GM was found in the Group 3 i.e., >30-50 years (9.44 mm ± 2.73 in the maxilla and 8.32 mm ± 1.7 in the mandible) and the maximum depth in the Group 4 (>50 years) (11.28 ± 2.31 in maxilla and 9.42 ± 2.87 in the mandible).</p><p><strong>Conclusion: </strong>The study provides a range of VD s in different areas of the mouth in periodontally healthy and individuals with GR, which will contribute to constitute a normal range of VD.</p>","PeriodicalId":15890,"journal":{"name":"Journal of Indian Society of Periodontology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of nonsurgical periodontal therapy on serum creatinine level in systemically healthy individuals with periodontitis: An interventional study.","authors":"Suchi Suvra Bagchi,&nbsp;Thamil Selvan Muthuraj,&nbsp;Puja Sarkar,&nbsp;Prasanta Bandyopadhyay,&nbsp;Papita Ghosh","doi":"10.4103/jisp.jisp_334_22","DOIUrl":"10.4103/jisp.jisp_334_22","url":null,"abstract":"<p><strong>Background: </strong>Several studies have suggested a relationship between periodontitis and serum creatinine levels. Both low and high serum creatinine levels have been reported in individuals with periodontitis. The impact of periodontal therapy on serum creatinine levels has not been fully investigated yet. The aim of the study is to estimate the influence of nonsurgical periodontal therapy (NSPT) on serum creatinine levels in systemically healthy individuals with periodontitis at stage II and stage III with grade A and grade B.</p><p><strong>Materials and methods: </strong>Sixty-eight systemically healthy individuals included in the study were divided into Group A (GA) (Periodontally healthy) and Group B (GB) (Periodontitis: stage II and III with grade A and grade B). Gingival index, sulcular bleeding index, probing pocket depth, clinical attachment level, body mass index, and serum creatinine levels were recorded at baseline for both GA and GB, 90 days after NSPT for GB only. Collected data were analyzed statistically.</p><p><strong>Results: </strong>Serum creatinine levels in GB were significantly higher when compared with GA and serum creatinine levels in GB before and 90 days after NSPT were statistically insignificant.</p><p><strong>Conclusions: </strong>Serum creatinine levels were higher in individuals with periodontitis when compared with periodontally healthy individuals and NSPT has no considerable influence on the serum creatinine levels in individuals with periodontitis. Further studies are required to confirm these findings.</p>","PeriodicalId":15890,"journal":{"name":"Journal of Indian Society of Periodontology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281315/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9709341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gingival recession: Narrow perception - erroneous; wider canvass - out-of-focus!","authors":"Ashish Kumar","doi":"10.4103/jisp.jisp_118_23","DOIUrl":"10.4103/jisp.jisp_118_23","url":null,"abstract":"","PeriodicalId":15890,"journal":{"name":"Journal of Indian Society of Periodontology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9709339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"R.I.P: The case sheet in periodontology.","authors":"Harpreet Singh Grover","doi":"10.4103/jisp.jisp_113_23","DOIUrl":"10.4103/jisp.jisp_113_23","url":null,"abstract":"","PeriodicalId":15890,"journal":{"name":"Journal of Indian Society of Periodontology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9684256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous detection and evaluation of <i>Prevotella intermedia, Prevotella nigrescens, Prevotella loescheii</i>, and <i>Prevotella melaninogenica</i> in subgingival plaque samples of chronic periodontitis and healthy individuals through multiplex polymerase chain reaction.","authors":"Vijaylakshmi S Kottrashetti,&nbsp;Kishore G Bhat,&nbsp;Manohar S Kugaji,&nbsp;Sachita Shrikant Naik,&nbsp;Praveen Tanakanti","doi":"10.4103/jisp.jisp_154_22","DOIUrl":"10.4103/jisp.jisp_154_22","url":null,"abstract":"<p><strong>Background: </strong><i>Prevotella</i> is a Gram-negative anaerobic bacilli. The phenotypic characteristics of the various species of <i>Prevotella</i> are similar, which often makes it difficult in routine differentiation and identification of all the species.</p><p><strong>Aim: </strong>The purpose of the study was to detect and compare presence of <i>Prevotella intermedia, Prevotella nigrescens, Prevotella melaninogenica</i>, and <i>Prevotella loescheii</i> in subgingival plaque samples of chronic periodontitis and healthy individuals.</p><p><strong>Materials and methods: </strong>Two hundred and thirty-six subjects were considered consisting of chronic periodontitis (128) and healthy (108) individuals. Subgingival plaque sample was collected in reduced transport fluid and analyzed. DNA extraction and polymerase chain reaction (PCR) were performed for genus <i>Prevotella</i> followed by positive samples were considered for the detection of selected species through multiplex PCR using specific primers.</p><p><strong>Results: </strong>Out of 236 samples, 94.1% were positive for genus <i>Prevotella</i>. Out of 222 cases <i>P. nigrescens</i> showed the highest number of cases positive (59.5%) followed by <i>P. melaninogenica</i> (57.2%), <i>P. intermedia</i> (55.4%), and <i>P. loescheii</i> (40.1%). Species were analyzed individually between chronic periodontitis and healthy, <i>P. intermedia, P. nigrescens</i>, and <i>P. loescheii</i> showed greater positivity in healthy compared to chronic periodontitis. Positivity for <i>P. melaninogenica</i> was high in chronic periodontitis compared to healthy.</p><p><strong>Conclusion: </strong>The number of positive cases for species, when correlated with clinical parameters showed an increase in mean score for all clinical parameters assessed, suggesting the presence of variation in the prevalence of <i>Prevotella</i> species and geographic variation do exist in oral microflora. Findings suggest that they can be normal commensals and opportunistic.</p>","PeriodicalId":15890,"journal":{"name":"Journal of Indian Society of Periodontology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9703099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlled release devices as adjuvants in nonsurgical periodontal therapy: A systematic review.","authors":"Samuel Chillavert Dias Pascoal,&nbsp;Maria Clara Ayres Estellita,&nbsp;Karlos Eduardo Rodrigues Lima,&nbsp;Eduardo da Cunha Queiroz,&nbsp;Talita Arrais Daniel Mendes","doi":"10.4103/jisp.jisp_50_22","DOIUrl":"10.4103/jisp.jisp_50_22","url":null,"abstract":"<p><p>Controlled release devices (CRD) have been widely studied regarding their application in periodontal therapy. Therefore, the present study aims to evaluate the use and effectiveness of controlled-release devices in nonsurgical periodontal therapy through a systematic review. The research was carried out in six different databases, namely: Online Medical Literature Search and Analysis System (Medline-PubMed), Web of Science, Science Direct, Scopus, Scielo, and Google Scholar. The descriptors \"Delayed-Action Preparation,\" \"Therapeutics,\" and \"Periodontitis,\" were used with their EntryTerms and connected through the Boolean operators AND and OR. A total of 2847 studies were found, and after applying the Preferred Reporting Items for Systematic Reviews and Meta-Analyses checklist flowchart, 34 were selected using the eligibility criteria. After that, the qualitative results were tabulated and the risk of bias in each of them was evaluated. It can be observed that the use of CRD presents itself as a successful alternative for adjuvant treatment to periodontal therapy, a fact due to its availability and local concentration in the crevicular fluid. However, further clinical research is still needed to develop devices that are effective, with an easy and quick application, as well as available at a good cost-benefit ratio.</p>","PeriodicalId":15890,"journal":{"name":"Journal of Indian Society of Periodontology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10281306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9714630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}