Journal of Fungi最新文献_第8页

Comparison of Rhizosphere Microbiomes Between Domesticated and Wild Wheat in a Typical Agricultural Field: Insights into Microbial Community Structure and Functional Shifts.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-20 DOI: 10.3390/jof11030168

Jie Fang, Mihal Blaschkauer, Assaf Distelfeld, Zihao Liu, Bin Song, Shimon Rachmilevitch, Jonathan M Adams

{"title":"Comparison of Rhizosphere Microbiomes Between Domesticated and Wild Wheat in a Typical Agricultural Field: Insights into Microbial Community Structure and Functional Shifts.","authors":"Jie Fang, Mihal Blaschkauer, Assaf Distelfeld, Zihao Liu, Bin Song, Shimon Rachmilevitch, Jonathan M Adams","doi":"10.3390/jof11030168","DOIUrl":"10.3390/jof11030168","url":null,"abstract":"While the differences between domesticated crops and their wild relatives have been extensively studied, less is known about their rhizosphere microbiomes, which hold potential for breeding stress-resistant traits. We compared the rhizosphere microbiomes of domesticated wheat (Triticum aestivum L.) and its wild ancestor (Triticum turgidum ssp. dicoccoides) in a typical agricultural field using 16S rRNA and ITS gene sequencing. Our results revealed a high level of conservation in the rhizosphere microbiomes between wild and domesticated wheat, with minimal divergence in community composition and microbial network structure. However, domesticated wheat exhibited a higher prevalence of fungal pathogens and increased functional redundancy, with significant enrichment of genes involved in carbon and nitrogen cycling. The microbial community assemblies in both wheats were predominantly governed by deterministic processes. This suggests that long-term conventional agricultural practices have imposed minor effects on the compositional differences between the microbiomes of wild and domesticated wheat. Nonetheless, the lower abundance of apparent pathogens in the rhizosphere of the wild wheat suggests greater natural biota or innate host plant resistance against pathogenic fungi. This study may provide valuable insights into the host selection, assembly patterns, and functional potential of microbial communities in wild versus domesticated wheat, with implications for manipulating microbial communities in future crop breeding.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungal Empyema Thoracis Associated with Clavispora lusitaniae-First Report in a Domestic Cat.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-20 DOI: 10.3390/jof11030170

Radka Garnoeva, Velina Dinkova

引用次数: 0

Integrated Multi-Omics Analysis to Investigate the Molecular Mechanisms Underlying the Response of Auricularia heimuer to High-Temperature Stress.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-20 DOI: 10.3390/jof11030167

Fang Lu, Xin Sun, Xiaodong Dai, Piqi Zhang, Yinpeng Ma, Yafei Xu, Lei Wang, Jiechi Zhang

{"title":"Integrated Multi-Omics Analysis to Investigate the Molecular Mechanisms Underlying the Response of Auricularia heimuer to High-Temperature Stress.","authors":"Fang Lu, Xin Sun, Xiaodong Dai, Piqi Zhang, Yinpeng Ma, Yafei Xu, Lei Wang, Jiechi Zhang","doi":"10.3390/jof11030167","DOIUrl":"10.3390/jof11030167","url":null,"abstract":"High-temperature stress is a key factor that reduces the yields of edible fungi. Auricularia heimuer (A. heimuer) is a nutrient-rich edible fungus that is widely cultivated in China. In this study, we analyzed the physiological, transcriptomic, and metabolomic results of A. heimuer (variety \"Hei29\") under high-temperature stress. Our findings revealed that high temperatures (30 °C and 35 °C) significantly reduced hyphal growth, increased malondialdehyde content and antioxidant enzyme activity, and enhanced the accumulation of secondary metabolites, such as phenolic compounds and flavonoids. A total of 15 candidate genes potentially responsive to high-temperature stress were identified through transcriptomic analysis, including those involved in regulating antioxidant defense, heat shock response, sugar metabolism, amino acid metabolism, and accumulating secondary metabolites. Metabolomic analysis identified three candidate metabolites potentially responsive to high-temperature stress, including kinetin, flavonoids, and caffeic acid, as well as several metabolic pathways, including nucleotide metabolism, ABC transporters, and cofactor biosynthesis. These mechanisms help mitigate oxidative damage to cellular structures and energy deficits caused by elevated temperatures, enabling the fungus to maintain cellular stability, metabolic function, and growth under heat stress. This study is the first to explore the molecular mechanism of A. heimuer in response to high-temperature stress. The results provide valuable insights into the molecular mechanisms of heat stress tolerance in A. heimuer, highlighting potential targets for developing heat-tolerant strains for industrial application.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943417/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taxonomic Revision of Solorina (Peltigeraceae, Ascomycota), Reveals a New Genus and Three New Species.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-20 DOI: 10.3390/jof11030169

Ting Zheng, Lisong Wang, Min Ai, Yuxin Gan, Rong Fan, Yingjun Zhang, Fiona Ruth Worthy, Jizhen Jin, Wenping Meng, Shengbang Zhang, Xinyu Wang

{"title":"Taxonomic Revision of Solorina (Peltigeraceae, Ascomycota), Reveals a New Genus and Three New Species.","authors":"Ting Zheng, Lisong Wang, Min Ai, Yuxin Gan, Rong Fan, Yingjun Zhang, Fiona Ruth Worthy, Jizhen Jin, Wenping Meng, Shengbang Zhang, Xinyu Wang","doi":"10.3390/jof11030169","DOIUrl":"10.3390/jof11030169","url":null,"abstract":"The lichen genus Solorina exhibits significant morphological and chemical variations between species. Recent molecular studies have demonstrated that Solorina is polyphyletic, underscoring the need for a comprehensive taxonomic revision. Phylogenetic analyses employing Bayesian methods and Maximum Likelihood approaches based on three molecular loci (nrITS, nrLSU, mtSSU) revealed that species of Solorina segregate into two distinct clades. The first clade includes species characterized by bright orange lower surfaces that contain secondary metabolites, notably solorinic acid. The type species, Solorina crocea, is retained in the genus Solorina. The second clade encompasses species with white or brownish lower surfaces; most species lack secondary metabolites and are now classified as a new genus, Pseudosolorina. As a result of this taxonomic revision, two species: S. crocea and S. crocoides remain in the genus Solorina. Five species with white or brownish lower surfaces were transferred to the new genus Pseudosolorina, which consists of three newly described species and five new combinations. Four species previously described as Solorina: S. embolina, S. fuegiensis, S. octospora, and S. platycarpa have morphology consistent with Pseudosolorina, but are currently retained in Solorina due to the absence of supporting DNA sequence data. A key to Solorina and Pseudosolorina is provided. The spores of S. crocea exhibit wall ornamentation featuring rounded papillae, which are distinct from those of Pseudosolorina. Molecular data and morphological characters also indicate that both Solorina and Pseudosolorina engage in symbiotic associations with photobionts cyanobacteria Nostoc and chlorophytes Coccomyxa or Asterochloris.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Haplotype-Phased Chromosome-Level Genome Assembly of Cryptoporus qinlingensis, a Typical Traditional Chinese Medicine Fungus.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-19 DOI: 10.3390/jof11020163

Yu Song, Ming Zhang, Yu-Ying Liu, Minglei Li, Xiuchao Xie, Jianzhao Qi

{"title":"Haplotype-Phased Chromosome-Level Genome Assembly of Cryptoporus qinlingensis, a Typical Traditional Chinese Medicine Fungus.","authors":"Yu Song, Ming Zhang, Yu-Ying Liu, Minglei Li, Xiuchao Xie, Jianzhao Qi","doi":"10.3390/jof11020163","DOIUrl":"10.3390/jof11020163","url":null,"abstract":"This study presents the first comprehensive genomic analysis of Cryptoporus qinlingensis, a classical folk medicine and newly identified macrofungus from the Qinling Mountains. Utilizing advanced sequencing technologies, including PacBio HiFi and Hi-C, we achieved a high-quality chromosome-level genome assembly. The genome, sized at 39.1 Mb, exhibits a heterozygosity of 0.21% and contains 21.2% repetitive sequences. Phylogenetic analysis revealed a recent divergence of C. qinlingensis from Dichomitus squalens approximately 212.26 million years ago (MYA), highlighting the rapid diversification within the Polyporaceae family. Comparative genomic studies indicate significant gene family contraction in C. qinlingensis, suggesting evolutionary adaptations. The identification of a tetrapolar mating system, along with the analysis of CAZymes and P450 genes, underscores the genomic complexity and ecological adaptability of this species. Furthermore, the discovery of 30 biosynthetic gene clusters (BGCs) related to secondary metabolites, including polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS), and terpene synthesis enzymes, opens new avenues for exploring bioactive compounds with potential medicinal applications. This research not only enriches our understanding of the Cryptoporus genus but also provides a valuable foundation for future studies aiming to harness the therapeutic potential of C. qinlingensis and to further explore its ecological and evolutionary significance.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and Functional Analysis of the Lectin-like Protein Llp1 Secreted by Ustilago maydis upon Infection of Maize.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-19 DOI: 10.3390/jof11020164

Marvin Christ, Itzel Rubio Elizalde, Paul Weiland, Antonia Kern, Thomas Iwen, Christopher-Nils Mais, Jan Pané-Farré, Stephan Kiontke, Florian Altegoer, Johannes Freitag, Gert Bange

{"title":"Structural and Functional Analysis of the Lectin-like Protein Llp1 Secreted by Ustilago maydis upon Infection of Maize.","authors":"Marvin Christ, Itzel Rubio Elizalde, Paul Weiland, Antonia Kern, Thomas Iwen, Christopher-Nils Mais, Jan Pané-Farré, Stephan Kiontke, Florian Altegoer, Johannes Freitag, Gert Bange","doi":"10.3390/jof11020164","DOIUrl":"10.3390/jof11020164","url":null,"abstract":"The biotrophic fungus Ustilago maydis, which causes smut disease in maize, secretes numerous proteins upon plant colonization. Some of them, termed effectors, help to evade plant defenses and manipulate cellular processes within the host. The function of many proteins specifically secreted during infection remains elusive. In this study, we biochemically characterized one such protein, UMAG_00027, that is highly expressed during plant infection. We show that UMAG_00027 is a secreted protein with a lectin-like fold and therefore term it Llp1 (lectin-like-protein 1). Llp1 decorated the fungal cell wall of cells grown in axenic culture or proliferating in planta, which is in agreement with its potential sugar-binding ability. We were unable to identify the precise sugar moieties that are bound by Llp1. CRISPR/Cas9-mediated deletion of llp1 reveals that the gene is not essential for fungal virulence. A structural search shows the presence of several other lectin-like proteins in U. maydis that might compensate for the function of Llp1 in ∆llp1 mutants. We therefore speculate that Llp1 is part of a family of lectin-like proteins with redundant functions.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Burden of Hospital Illness Associated with Disseminated Versus Isolated Pulmonary Coccidioidomycosis in the United States.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-19 DOI: 10.3390/jof11020161

Craig I Coleman, Jessica Bylyku, Andria Latifi, Belinda Lovelace, Ryan Shan, Lahar Miriyapalli, Fariba Donovan

{"title":"The Burden of Hospital Illness Associated with Disseminated Versus Isolated Pulmonary Coccidioidomycosis in the United States.","authors":"Craig I Coleman, Jessica Bylyku, Andria Latifi, Belinda Lovelace, Ryan Shan, Lahar Miriyapalli, Fariba Donovan","doi":"10.3390/jof11020161","DOIUrl":"10.3390/jof11020161","url":null,"abstract":"There are scarce data comparing inpatient mortality, length of stay (LOS) and all-cause hospital costs in disseminated coccidioidomycosis (DCM) vs. isolated pulmonary coccidioidomycosis (IPCM). We assessed the burden of hospital illness associated with DCM versus IPCM. This study was performed using National Inpatient Sample data from 2019 to 2021. DCM was defined as having a primary International Classification of Diseases-Tenth Revision (ICD-10) code for coccidioidal meningitis, a non-primary code for coccidioidal meningitis in the presence of a primary code for a meningitis complication or a procedure code depicting the need for a meningitis-related procedure, or a primary code for DCM without a code for unspecified disease. IPCM was defined as a primary code for pulmonary coccidioidomycosis without codes for DCM or unspecified disease. Multivariable regression was used to compare the odds of in-hospital mortality, LOS and all-cause hospital costs (2023 US$) for DCM versus IPCM, after covariate adjustment. A total of 6195 hospitalizations were identified, 2305 for DCM and 3890 for IPCM. Patients experiencing a DCM hospitalization had a 19.7% incidence of concomitant pulmonary coccidioidomycosis. Coccidioidal meningitis constituted 81.3% of all DCM hospitalizations, of which 78.1% received a meningitis-related procedure or were admitted for a meningitis complication. DCM was associated with an increased odds of death (odds ratio = 2.76, 95% confidence interval [CI] = 1.26-6.04) versus IPCM. DCM was associated with a longer mean hospital LOS (4.51 days, 95%CI = 3.39-5.63) and higher mean all-cause costs ($20,008, 95%CI = $15,313-$24,704) versus IPCM. DCM hospitalizations were associated with higher odds of inpatient mortality, longer LOS, and higher costs versus IPCM.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Outcome Analysis of Breakthrough Invasive Aspergillosis on Anti-Mold Azole Prophylaxis and Treatment: 30-Year Experience in Hematologic Malignancy Patients.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-19 DOI: 10.3390/jof11020160

Hiba Dagher, Anne-Marie Chaftari, Andrea Haddad, Ying Jiang, Jishna Shrestha, Robin Sherchan, Peter Lamie, Jennifer Makhoul, Patrick Chaftari, Ray Hachem, Issam Raad

{"title":"Outcome Analysis of Breakthrough Invasive Aspergillosis on Anti-Mold Azole Prophylaxis and Treatment: 30-Year Experience in Hematologic Malignancy Patients.","authors":"Hiba Dagher, Anne-Marie Chaftari, Andrea Haddad, Ying Jiang, Jishna Shrestha, Robin Sherchan, Peter Lamie, Jennifer Makhoul, Patrick Chaftari, Ray Hachem, Issam Raad","doi":"10.3390/jof11020160","DOIUrl":"10.3390/jof11020160","url":null,"abstract":"Background: Anti-mold azoles have improved the outcomes of invasive aspergillosis (IA) when used therapeutically, but they are extensively used as prophylaxis. There are limited data regarding the outcomes of patients with hematologic malignancy who develop breakthrough IA on anti-mold azoles. We aimed to determine whether breakthrough IA on azole prophylaxis shows worse outcomes compared to no prophylaxis.Methods: We compared outcomes including therapy response and mortality between antifungal regimens in hematologic malignancy patients with IA between July 1993 and July 2023.Results: Compared to an amphotericin B-containing regimen (AMB), an anti-mold azole as the primary therapy was independently associated with successful response at the end of therapy (OR = 4.38, p < 0.0001), protective against 42-day IA-associated mortality (OR = 0.51, p = 0.024) or all cause mortality (OR = 0.35, p < 0.0001), and protective against 84-day mortality, both IA-associated (OR = 0.50, p = 0.01) and all-cause mortality (OR = 0.27, p < 0.0001). Azole prophylaxis was independently associated with higher IA-associated mortality at 42 days (OR = 1.91, p = 0.012) and 84 days (OR = 2.03, p = 0.004), compared to fluconazole or no prophylaxis.Conclusions: Patients with breakthrough IA on anti-mold azole prophylaxis show a worse prognosis than those on other or no prophylaxis, possibly related to the emergence of azole resistance due to their widespread use as prophylaxis agents. On the other hand, anti-mold azole primary therapy is superior to AMB therapy in the treatment of IA.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal Policy and Practice Across Five Countries: A Qualitative Review.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-19 DOI: 10.3390/jof11020162

David W Denning, John R Perfect, Neda Milevska-Kostova, Artes Haderi, Hannah Armstrong, Maarten C Hardenberg, Emily Chavez, Bruce Altevogt, Patrick Holmes, Jalal A Aram

{"title":"Antifungal Policy and Practice Across Five Countries: A Qualitative Review.","authors":"David W Denning, John R Perfect, Neda Milevska-Kostova, Artes Haderi, Hannah Armstrong, Maarten C Hardenberg, Emily Chavez, Bruce Altevogt, Patrick Holmes, Jalal A Aram","doi":"10.3390/jof11020162","DOIUrl":"10.3390/jof11020162","url":null,"abstract":"The burden of invasive fungal infections (IFIs) is increasing worldwide. National, regional, and local policies on IFI management should respond to the changing landscape. We assessed antifungal policies from five countries of varying size, IFI burden, and geography: the Netherlands, Italy, South Korea, China, and India. These countries were selected as a representative sample reflecting different types of economic and health systems that patients and providers access worldwide. This assessment focused on a comprehensive range of antifungal policy elements, including recognition and prioritization, awareness and education, prevention and monitoring, diagnosis and coordinated care, access to appropriate treatment, and diagnostic and treatment innovation. Although countries in this analysis all have some form of policy for IFI management, we have identified substantial gaps, including low prioritization of IFI diagnostics, omission of fungal pathogens from antimicrobial resistance policies, and a general lack of awareness and healthcare professional (HCP) training on IFI management. The gaps identified are intended to inform HCPs and policy- and decision-makers about aspects to consider in reducing the IFI burden for patients and health systems while demonstrating responsible antifungal stewardship.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of Antagonistic Trichoderma Strains to Enhance Soybean Growth.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-19 DOI: 10.3390/jof11020159

Na Yu, Yijia Gao, Feng Chang, Wenting Liu, Changhong Guo, Hongsheng Cai

{"title":"Screening of Antagonistic Trichoderma Strains to Enhance Soybean Growth.","authors":"Na Yu, Yijia Gao, Feng Chang, Wenting Liu, Changhong Guo, Hongsheng Cai","doi":"10.3390/jof11020159","DOIUrl":"10.3390/jof11020159","url":null,"abstract":"This study investigates the isolation and screening of Trichoderma strains that exhibit antagonistic properties against soybean root-infecting Fusarium species, particularly F. oxysporum. From soybean rhizosphere soil, 37 antagonistic Trichoderma strains were identified using the plate confrontation method, demonstrating inhibitory effects ranging from 47.57% to 72.86% against F. oxysporum. Strain 235T4 exhibited the highest inhibition rate at 72.86%. Molecular identification confirmed that the strains belonged to eight species within the Trichoderma genus, with notable strains promoting soybean growth in greenhouse tests. In pot experiments, the application of Trichoderma significantly reduced the disease index of soybean plants inoculated with F. oxysporum, particularly with strain 223H16, which achieved an 83.78% control efficiency. Field applications further indicated enhanced soybean growth metrics, including increased pod numbers and plant height, when treated with specific Trichoderma strains. Additionally, Trichoderma application enriched the fungal diversity in the soybean rhizosphere, resulting in a significant reduction of Fusarium populations by approximately 50%. This study highlights the potential of Trichoderma species as biological control agents to enhance soybean health and productivity while improving soil fungal diversity.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0