Journal of Fungi最新文献

{"title":"<i>Galleria mellonella</i> as an Invertebrate Model for Studying Fungal Infections.","authors":"Gabriel Davi Marena, Luciana Thomaz, Joshua Daniel Nosanchuk, Carlos Pelleschi Taborda","doi":"10.3390/jof11020157","DOIUrl":"10.3390/jof11020157","url":null,"abstract":"<p><p>The incidence of fungal infections continues to increase and one of the factors responsible for these high rates is the emergence of multi-resistant species, hospitalizations, inappropriate or prolonged use of medications, and pandemics, such as the ongoing HIV/AIDS pandemic. The recent pandemic caused by the severe acute respiratory syndrome virus (SARS-CoV-2) has led to a significant increase in fungal infections, especially systemic mycoses caused by opportunistic fungi. There is a growing and urgent need to better understand how these microorganisms cause infection and develop resistance as well as to develop new therapeutic strategies to combat the diverse diseases caused by fungi. Non-mammalian hosts are increasingly used as alternative models to study microbial infections. Due to their low cost, simplicity of care, conserved innate immunity and reduced ethical issues, the greater wax moth <i>Galleria mellonella</i> is an excellent model host for studying fungal infections and it is currently widely used to study fungal pathogenesis and develop innovative strategies to mitigate the mycoses studied. <i>G. mellonella</i> can grow at 37 °C, which is similar to the mammalian temperature, and the anatomy of the larvae allows researchers to easily deliver pathogens, biological products, compounds and drugs. The aim of this review is to describe how <i>G. mellonella</i> is being used as a model system to study fungal infections as well as the importance of this model in evaluating the antifungal profile of potential drug candidates or new therapies against fungi.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal-Induced Hemophagocytic Lymphohistiocytosis: A Literature Review in Non-HIV Populations.","authors":"Chia-Yu Chiu, Rachel S Hicklen, Dimitrios P Kontoyiannis","doi":"10.3390/jof11020158","DOIUrl":"10.3390/jof11020158","url":null,"abstract":"<p><p>We performed a thorough search of the literature published through December 2024 with no date exclusions on invasive fungal infection (IFI)-induced hemophagocytic lymphohistiocytosis (HLH) in non-human immunodeficiency virus (HIV) patients. The frequency of IFI-induced HLH reported across 16 articles was 9%. Of the 116 identified cases with available clinical information, 53% occurred in immunocompromised patients. IFIs were usually disseminated (76%), with <i>Histoplasma capsulatum</i> being the most common pathogen (51%). IFI and HLH were diagnosed simultaneously in most cases (78%). The 30-day survival rate was 64%. Reported cases had significant heterogeneity in patient characteristics, management strategies, and outcomes.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species Diversity and Community Composition of Macrofungi in the Dongling Mountains, Western Beijing, China.","authors":"Jia-Mao Huang, Tai-Min Xu, Wen Zhao, Reyila Mumin, Long Zeng, Yi-Fei Sun, Bao-Kai Cui","doi":"10.3390/jof11020155","DOIUrl":"10.3390/jof11020155","url":null,"abstract":"<p><p>Macrofungi are a group of fungi with important ecological functions and economic value in forest ecosystems. In this study, the species diversity and community composition of macrofungi in different areas, vegetation types, and elevation gradients from the Lingshan Natural Scenic Area and Xiaolongmen National Forest Park of the Dongling Mountains, Western Beijing were investigated. A total of 1379 specimens were collected. All specimens were identified by morphological and molecular methods, resulting in the identification of 311 species belonging to two phyla, four classes, 18 orders, 74 families, and 147 genera. The alpha diversity analysis indicated that the alpha diversity was significantly different among different vegetation types. The <i>Betula</i> spp. and <i>Populus davidiana</i> of the Lingshan Natural Scenic Area, and <i>Quercus liaotungensis</i> of Xiaolongmen National Forest Park had higher macrofungal alpha diversity. The difference in alpha diversity between the two areas was not significant. The principal coordinate analysis indicated a significant difference in macrofungal community composition among different vegetation types. The fungal community composition of the two areas was also significantly different. The fungal species richness and unique species tended to increase and then decrease with increasing elevation. The species composition of neighboring elevation gradients was more similar. The macrofungal species richness and the number of unique species were not significantly affected by elevation gradient. The vegetation types with higher fungal alpha diversity in the Dongling Mountains were <i>Betula</i> spp., <i>Populus davidiana</i>, and <i>Quercus liaotungensis</i>, and there were abundant fungal species in deciduous broad-leaved mixed forests. Macrofungal diversity and community composition was significantly affected by vegetation type. To better protect the macrofungi, the protection of these four vegetation types in the Dongling Mountains should be strengthened in the future.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Infection Model Using A6 Cells Sets the Stage for Host-<i>Batrachochytrium salamandrivorans</i> Exploration.","authors":"Elin Verbrugghe, Frank Pasmans, An Martel","doi":"10.3390/jof11020156","DOIUrl":"10.3390/jof11020156","url":null,"abstract":"<p><p>The chytrid fungus <i>Batrachochytrium salamandrivorans</i> (Bsal) poses a significant threat to amphibian biodiversity, driving severe declines in salamander populations in Europe. While understanding the host-pathogen interaction may yield novel avenues for disease mitigation, effective in vitro models are currently lacking. We here develop a cell-culture-based model using A6 cells to reproduce the complete life cycle of Bsal in vitro, encompassing key stages such as β-galactose-associated cell attachment, active host cell penetration, intracellular maturation, host cell death, and Bsal release. Using imaging techniques, we provide evidence that Bsal penetrates A6 cells through a mechanism independent of conventional host actin dynamics. Our comparative analysis reveals that Bsal infection closely mirrors responses observed in native salamander skin tissues, validating the A6 cell line as an effective surrogate for in vivo studies. This research enhances our understanding of Bsal's pathogenicity and emphasizes the potential of the A6 cell model for future studies.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significant Enrichment of Potential Pathogenic Fungi in Soil Mediated by Flavonoids, Phenolic Acids, and Organic Acids.","authors":"Shaoguan Zhao, Yan Sun, Lanxi Su, Lin Yan, Xingjun Lin, Yuzhou Long, Ang Zhang, Qingyun Zhao","doi":"10.3390/jof11020154","DOIUrl":"10.3390/jof11020154","url":null,"abstract":"<p><p>It is well established that root exudates play a crucial role in shaping the assembly of plant rhizosphere microbial communities. Nonetheless, our understanding of how different types of exudates influence the abundance of potential pathogens in soil remains insufficient. Investigating the effects of root exudates on soil-dwelling pathogenic fungi is imperative for a comprehensive understanding of plant-fungal interactions within soil ecosystems and for maintaining soil health. This study aimed to elucidate the effects of the principal components of root exudates-flavonoids (FLA), phenolic acids (PA), and organic acids (OA)-on soil microbial communities and soil properties, as well as to investigate their mechanisms of action on soil potential pathogenic fungi. The results demonstrated that the addition of these components significantly modified the composition and diversity of soil microbial communities, with OA treatment notably altering the composition of dominant microbial taxa. Furthermore, the introduction of these substances facilitated the proliferation of saprophytic fungi. Additionally, the incorporation of flavonoids, phenolic acids, and organic acids led to an increased abundance of potential pathogenic fungi in the soil, particularly in the FLA and PA treatments. It was observed that the addition of these substances enhanced soil fertility, pH, and antioxidant enzyme activity. Specifically, FLA and PA treatments reduced the abundance of dominant microbial taxa, whereas OA treatment altered the composition of these taxa. These findings suggest that the inclusion of flavonoids, phenolic acids, and organic acids could potentially augment the enrichment of soil potential pathogenic fungi by modulating soil properties and enzymatic activities. These results offer valuable insights into the interactions between plants and fungal communities in soil ecosystems and provide a scientific foundation for the management and maintenance of soil health.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Candidemia in ICU Patients: What Are the Real Game-Changers for Survival?","authors":"Bianca Leal de Almeida, Caroline Agnelli, Thaís Guimarães, Teresa Sukiennik, Paulo Roberto Passos Lima, Mauro José Costa Salles, Giovanni Luís Breda, Flavio Queiroz-Telles, Ana Verena Almeida Mendes, Luís Fernando Aranha Camargo, Hugo Manuel Paz Morales, Viviane Maria de Carvalho Hessel Dias, Afonso Rafael da Silva Junior, João Nóbrega de Almeida Junior, Camila de Melo Picone, Evangelina da Motta Pacheco Alves de Araújo, Edson Abdala, Flávia Rossi, Arnaldo Lopes Colombo, Marcello Mihailenko Chaves Magri","doi":"10.3390/jof11020152","DOIUrl":"10.3390/jof11020152","url":null,"abstract":"<p><p>Candidemia infection remains a critical challenge in intensive care units (ICUs), with high morbidity and mortality rates despite advances in therapeutic practices. This multicenter prospective surveillance study assessed the epidemiology, clinical management, and mortality predictors of candidemia in critically ill patients across two periods (2010-2012 and 2017-2018) in 11 tertiary hospitals in Brazil. Among 314 ICU patients with candidemia, the overall mortality rate was 60.2%, with no significant reduction over time (58.8% vs. 62.6%, <i>p</i> = 0.721). <i>Candida albicans</i> was the predominant pathogen (43.6%), followed by <i>C. tropicalis</i> (20%) and <i>C. glabrata</i> (13.7%). The use of echinocandins increased significantly in the second period (21.1% to 41.7%, <i>p</i> < 0.001); however, 70% of patients still did not receive these agents as first-line therapy. Catheter removal due to candidemia was performed in only 52.1% of cases but was associated with improved 30-day survival (<i>p</i> < 0.001). Multivariate analysis identified cancer, inadequate treatment, and vasoactive drug use as independent predictors of mortality. Our findings underscore persistent gaps in adherence to guidelines, particularly regarding timely echinocandin initiation and catheter removal. Strengthening therapeutic strategies focused on these key interventions is essential to improving outcomes for ICU patients with candidemia.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Analysis of <i>Penicillium griseofulvum</i> CF3 Reveals Potential for Plant Growth Promotion and Disease Resistance.","authors":"Jianfei Yang, Wenshuai Zang, Jie Chen, Dongying Lu, Ruotong Li, Ciyun Li, Yinhua Chen, Qin Liu, Xiaolei Niu","doi":"10.3390/jof11020153","DOIUrl":"10.3390/jof11020153","url":null,"abstract":"<p><p><i>Penicillium griseofulvum</i> CF3 is a fungus isolated from healthy strawberry soil, with the potential to promote the growth of plants and enhance their resistance to diseases. However, the genome sequence of <i>P. griseofulvum</i> CF3 remains unclear. Therefore, we performed the whole-genome CCS sequencing of <i>P. griseofulvum</i> CF3 using the PacBio Sequel II platform. The assembled genome comprised 104 contigs, with a total length of 37,564,657 bp, encoding 13,252 protein-coding genes. Comprehensive functional annotation was performed using various BLAST databases, including the non-redundant (Nr) protein sequence database, Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG), EuKaryotic Orthologous Groups (KOG), and the Carbohydrate-Active enZymes (CAZy) database, to identify and predict protein-coding genes, tRNAs, and rRNAs. The Antibiotics and Secondary Metabolites Analysis Shell (Antismash) analysis identified 50 biosynthetic gene clusters involved in secondary metabolite production within the <i>P. griseofulvum</i> CF3 genome. The whole-genome sequencing of <i>P. griseofulvum</i> CF3 helps us to understand its potential mechanisms in promoting plant growth and enhancing disease resistance, paving the way for the application of the CF3 strain in sustainable crop production.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SsNEP2 Plays a Role in the Interaction Between <i>Sclerotinia sclerotiorum</i> and <i>Coniothyrium minitans</i>.","authors":"Huizhang Zhao, Zihang Zhu, Yueli Xu, Haixuan Wang, Jiatao Xie, Jiasen Cheng, Daohong Jiang, Yanping Fu","doi":"10.3390/jof11020151","DOIUrl":"10.3390/jof11020151","url":null,"abstract":"<p><p><i>Sclerotinia sclerotiorum</i>, a fungal pathogen that is spread worldwide and causes serious diseases on crops, can be parasitized specifically by the mycoparasite <i>Coniothyrium minitans</i>. <i>SsNEP2,</i> encoding a necrosis-inducing protein in <i>S. sclerotiorum,</i> was previously inferred to play a role in the virulence to host plants. In this study, silencing of <i>SsNEP2</i> in <i>S. sclerotiorum</i> had no significant (<i>p</i> < 0.01) influence on mycelial morphology, while overexpression led to lower mycelial growth and more branches. When amended with the fermentation broth of the <i>SsNEP2</i> silencing mutants, conidial germination of <i>C. minitans</i> was promoted, while conidial production decreased. When parasitized by <i>C. minitans</i>, enhanced resistance of the <i>SsNEP2</i> silencing mutants and weaker resistance of the overexpressed transformants were observed compared to the wild-type <i>S. sclerotiorum</i> strain 1980. In addition, the expression of <i>SsNEP2</i> in <i>C. minitans</i> enhanced mycelial parasitism on <i>S. sclerotiorum</i> and restored the effect of silencing <i>SsNEP2</i> in <i>S. sclerotiorum</i> on mycoparasitism. Thus, we highlight the role of <i>SsNEP2</i> as a PAMP-like protein in the mycoparasitism between <i>C. minitans</i> and its host fungus <i>S. sclerotiorum. SsNEP2</i> can be used to promote the biological potential of <i>C. minitans</i>.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856156/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species-Specific Sensitivity and Levels of Beta-D-Glucan for the Diagnosis of Candidemia-A Systematic Review and Meta-Analysis.","authors":"Nadir Ullah, Marco Muccio, Laura Magnasco, Chiara Sepulcri, Daniele Roberto Giacobbe, Antonio Vena, Matteo Bassetti, Malgorzata Mikulska","doi":"10.3390/jof11020149","DOIUrl":"10.3390/jof11020149","url":null,"abstract":"<p><strong>Background: </strong>1, 3-ß-D-Glucan (BDG) is an antigen present in the cell wall of many pathogenic fungi and is used as a marker for the early diagnosis of candidemia and discontinuation of empirical treatment. Changes in the epidemiology of <i>Candida</i> species might have a negative impact on the performance of serum BDG. The aim of this study was to analyze the performance of BDG in candidemia diagnosis focusing on species-specific differences in BDG sensitivity and BDG levels.</p><p><strong>Methods: </strong>The PRISMA system was used for the systematic search. The following databases were searched for articles published from January 2010 to November 2023: PubMed, Science Direct, and Scopus.</p><p><strong>Results: </strong>A total of 21 studies that met the inclusion criteria were included, reporting data from 1633 patients with candidemia; 11 reported both sensitivity and specificity, 15 reported species-specific sensitivity, and nine reported species-specific BDG levels. The pooled sensitivity of BDG in all studies was 0.73 (95% confidence interval (CI), 0.66-0.80), while the pooled sensitivity and specificity in 11 studies were 0.81 (95% CI 0.73-0.89) and 0.80 (95% CI 0.74-0.87). BDG pooled sensitivity (all assays) and BDG levels (for assays with cutoff of 80 pg/mL) were the highest in <i>C. krusei</i> (currently <i>Pichia kudriavzevii</i>) and the lowest in <i>C. auris</i>: 0.76 and 417 pg/mL for <i>C. krusei</i>, 0.73 and 345 pg/mL for <i>C. albicans</i>, 0.74 and 356 pg/mL for <i>C. glabrata</i> (currently <i>Nakaseomyces glabrata</i>), 0.70 and 324 pg/mL for <i>C. tropicalis</i>, 0.63 and 95 pg/mL for <i>C. parapsilosis,</i> 0.51 and 62 pg/mL for <i>C. auris,</i> and 0.44 and 79 pg/mL for other <i>Candida</i> species. These differences were statistically significant for BDG sensitivity and levels of <i>C. albicans</i>, <i>C. glabrata</i>, <i>C. krusei</i>, and <i>C. tropicalis</i> compared to <i>C. auris</i>, <i>C. parapsilosis</i>, and other <i>Candida</i> species.</p><p><strong>Conclusion: </strong>The sensitivity of BDG in candidemia diagnosis depends on the <i>Candida</i> species, with the lowest being for <i>C. auris</i> and <i>C. parapsilosis</i>. This might have a clinical impact in centers where these species are prevalent.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-Dimensional Chromatographic Isolation of High Purity Erinacine A from <i>Hericium erinaceus</i>.","authors":"Katerina Naumoska, Andrej Gregori, Alen Albreht","doi":"10.3390/jof11020150","DOIUrl":"10.3390/jof11020150","url":null,"abstract":"<p><p>A simple and robust two-dimensional chromatographic fractionation protocol for the isolation of the neuroprotective compound erinacine A from <i>Hericium erinaceus</i> is proposed. This production platform yielded 19.4 mg of erinacine A from approximately 130 g of mushroom material, with a chromatographic purity of 97.4%. The procedure includes extraction, concentration, fractionation, purification, and characterisation of the bioactive compound. The crude <i>H. erinaceus</i> extract was fractionated in the first dimension by normal-phase flash chromatography, and the fraction containing erinacine A was further purified in the second dimension by semi-preparative reversed-phase chromatography. This strategy utilises the orthogonality of the two chromatographic modes to effectively eliminate difficult impurities, including structural isomers and analogues of erinacine A. Complementary analytical approaches such as high-performance thin-layer chromatography (HPTLC) and high-performance liquid chromatography with ultraviolet and tandem mass spectrometric detection (HPLC-UV-MS/MS) were employed to unambiguously confirm erinacine A in the isolated fractions, while HPLC with a charged aerosol detector (CAD) was used to determine its purity. Given the limited commercial availability and the high price of erinacine A, the described method offers a straightforward and cost-effective alternative to obtain this valuable compound for further research and applications.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}