Alternaria alternata botybirnavirus 1 (AaBRV1) Infection Affects the Biological Characteristics of Its Host Fungus Alternaria alternata.

IF 4.2 2区生物学 Q2 MICROBIOLOGY

Journal of Fungi Pub Date : 2025-05-15 DOI:10.3390/jof11050376

Xinyi Zhang, Qiqi Zhu, Ziyuan Chen, Ju Chen, Zhijun Liu, Xuehong Wu

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引用次数: 0

Abstract

A botybirnavirus, Alternaria alternata botybirnavirus 1 (AaBRV1), had been identified from Alternaria alternata strain SD-BZF-19 isolated from diseased watermelon leaves in our previous study. In the current study, AaBRV1 was eliminated from its host fungus strain SD-BZF-19 using single hyphal tip culture method combined with high-temperature treatment to obtain the AaBRV1-free strain, which was named SD-BZF-19-G14. Compared with strain SD-BZF-19-G14, following AaBRV1 infection, colony color of strain SD-BZF-19 changed, and colony growth rate, dry weight of mycelial biomass, and sensitivity to difenoconazole, fludioxonil, and tebuconazole of strain SD-BZF-19 all decreased. However, the virulence of strain SD-BZF-19 was not significantly different from strain SD-BZF-19-G14, with disease index of watermelon leaves inoculated with SD-BZF-19 and SD-BZF-19-G14 being 90.83 and 81.67, respectively. A total of 1244 differentially expressed genes (DEGs) were identified in a comparative transcriptome analysis between the two strains, SD-BZF-19 and SD-BZF-19-G14. Relative to strain SD-BZF-19, the number of upregulated and downregulated DEGs in strain SD-BZF-19-G14 was 660 and 584, respectively. Notably, Pfam database annotated that the number of DEGs related to Major Facilitator Superfamily (MFS) and Cytochrome P450 (CYP450) was 36 and 28, respectively. To our knowledge, this is the first documentation of biological characteristics induced by AaBRV1 infection in A. alternata.

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互交黑穗病双联病毒1型（AaBRV1）感染对寄主真菌互交黑穗病生物学特性的影响

本研究从西瓜病叶中分离得到一株互花疫病毒SD-BZF-19，并鉴定出一株互花疫病毒1型（AaBRV1）。本研究采用单菌丝尖端培养结合高温处理的方法，从宿主真菌SD-BZF-19中去除AaBRV1，获得无AaBRV1的菌株，命名为SD-BZF-19- g14。与菌株SD-BZF-19- g14相比，感染AaBRV1后，菌株SD-BZF-19菌落颜色发生变化，菌落生长率、菌丝生物量干重、对二苯醚康唑、氟菌腈和戊康唑的敏感性均降低。而菌株SD-BZF-19的毒力与菌株SD-BZF-19- g14无显著差异，接种SD-BZF-19和SD-BZF-19- g14后西瓜叶片病害指数分别为90.83和81.67。对SD-BZF-19和SD-BZF-19- g14菌株进行转录组比较分析，共鉴定出1244个差异表达基因（DEGs）。相对于菌株SD-BZF-19，菌株SD-BZF-19- g14的deg上调和下调数量分别为660和584个。值得注意的是，Pfam数据库注释了与主要促进者超家族（MFS）和细胞色素P450 （CYP450）相关的deg数量分别为36和28。据我们所知，这是第一次记录了由AaBRV1感染引起的交替田鼠的生物学特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Fungi Medicine-Microbiology (medical)

CiteScore

6.70

自引率

14.90%

发文量

1151

审稿时长

11 weeks

期刊介绍： Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.