Journal of Fungi最新文献

{"title":"Correction: Ellsworth, M.; Ostrosky-Zeichner, L. Isavuconazole: Mechanism of Action, Clinical Efficacy, and Resistance. <i>J. Fungi</i> 2020, <i>6</i>, 324.","authors":"Misti Ellsworth, Luis Ostrosky-Zeichner","doi":"10.3390/jof11030226","DOIUrl":"https://doi.org/10.3390/jof11030226","url":null,"abstract":"<p><p>In the original article [...].</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haplotype-Phased Chromosome-Level Genome Assembly of <i>Cryptoporus qinlingensis</i>, a Typical Traditional Chinese Medicine Fungus.","authors":"Yu Song, Ming Zhang, Yu-Ying Liu, Minglei Li, Xiuchao Xie, Jianzhao Qi","doi":"10.3390/jof11020163","DOIUrl":"10.3390/jof11020163","url":null,"abstract":"<p><p>This study presents the first comprehensive genomic analysis of <i>Cryptoporus qinlingensis</i>, a classical folk medicine and newly identified macrofungus from the Qinling Mountains. Utilizing advanced sequencing technologies, including PacBio HiFi and Hi-C, we achieved a high-quality chromosome-level genome assembly. The genome, sized at 39.1 Mb, exhibits a heterozygosity of 0.21% and contains 21.2% repetitive sequences. Phylogenetic analysis revealed a recent divergence of <i>C. qinlingensis</i> from <i>Dichomitus squalens</i> approximately 212.26 million years ago (MYA), highlighting the rapid diversification within the Polyporaceae family. Comparative genomic studies indicate significant gene family contraction in <i>C. qinlingensis</i>, suggesting evolutionary adaptations. The identification of a tetrapolar mating system, along with the analysis of CAZymes and P450 genes, underscores the genomic complexity and ecological adaptability of this species. Furthermore, the discovery of 30 biosynthetic gene clusters (BGCs) related to secondary metabolites, including polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS), and terpene synthesis enzymes, opens new avenues for exploring bioactive compounds with potential medicinal applications. This research not only enriches our understanding of the <i>Cryptoporus</i> genus but also provides a valuable foundation for future studies aiming to harness the therapeutic potential of <i>C. qinlingensis</i> and to further explore its ecological and evolutionary significance.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural and Functional Analysis of the Lectin-like Protein Llp1 Secreted by <i>Ustilago maydis</i> upon Infection of Maize.","authors":"Marvin Christ, Itzel Rubio Elizalde, Paul Weiland, Antonia Kern, Thomas Iwen, Christopher-Nils Mais, Jan Pané-Farré, Stephan Kiontke, Florian Altegoer, Johannes Freitag, Gert Bange","doi":"10.3390/jof11020164","DOIUrl":"10.3390/jof11020164","url":null,"abstract":"<p><p>The biotrophic fungus <i>Ustilago maydis</i>, which causes smut disease in maize, secretes numerous proteins upon plant colonization. Some of them, termed effectors, help to evade plant defenses and manipulate cellular processes within the host. The function of many proteins specifically secreted during infection remains elusive. In this study, we biochemically characterized one such protein, UMAG_00027, that is highly expressed during plant infection. We show that UMAG_00027 is a secreted protein with a lectin-like fold and therefore term it Llp1 (lectin-like-protein 1). Llp1 decorated the fungal cell wall of cells grown in axenic culture or proliferating in planta, which is in agreement with its potential sugar-binding ability. We were unable to identify the precise sugar moieties that are bound by Llp1. CRISPR/Cas9-mediated deletion of <i>llp1</i> reveals that the gene is not essential for fungal virulence. A structural search shows the presence of several other lectin-like proteins in <i>U. maydis</i> that might compensate for the function of Llp1 in ∆<i>llp1</i> mutants. We therefore speculate that Llp1 is part of a family of lectin-like proteins with redundant functions.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Burden of Hospital Illness Associated with Disseminated Versus Isolated Pulmonary Coccidioidomycosis in the United States.","authors":"Craig I Coleman, Jessica Bylyku, Andria Latifi, Belinda Lovelace, Ryan Shan, Lahar Miriyapalli, Fariba Donovan","doi":"10.3390/jof11020161","DOIUrl":"10.3390/jof11020161","url":null,"abstract":"<p><p>There are scarce data comparing inpatient mortality, length of stay (LOS) and all-cause hospital costs in disseminated coccidioidomycosis (DCM) vs. isolated pulmonary coccidioidomycosis (IPCM). We assessed the burden of hospital illness associated with DCM versus IPCM. This study was performed using National Inpatient Sample data from 2019 to 2021. DCM was defined as having a primary International Classification of Diseases-Tenth Revision (ICD-10) code for coccidioidal meningitis, a non-primary code for coccidioidal meningitis in the presence of a primary code for a meningitis complication or a procedure code depicting the need for a meningitis-related procedure, or a primary code for DCM without a code for unspecified disease. IPCM was defined as a primary code for pulmonary coccidioidomycosis without codes for DCM or unspecified disease. Multivariable regression was used to compare the odds of in-hospital mortality, LOS and all-cause hospital costs (2023 US$) for DCM versus IPCM, after covariate adjustment. A total of 6195 hospitalizations were identified, 2305 for DCM and 3890 for IPCM. Patients experiencing a DCM hospitalization had a 19.7% incidence of concomitant pulmonary coccidioidomycosis. Coccidioidal meningitis constituted 81.3% of all DCM hospitalizations, of which 78.1% received a meningitis-related procedure or were admitted for a meningitis complication. DCM was associated with an increased odds of death (odds ratio = 2.76, 95% confidence interval [CI] = 1.26-6.04) versus IPCM. DCM was associated with a longer mean hospital LOS (4.51 days, 95%CI = 3.39-5.63) and higher mean all-cause costs ($20,008, 95%CI = $15,313-$24,704) versus IPCM. DCM hospitalizations were associated with higher odds of inpatient mortality, longer LOS, and higher costs versus IPCM.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcome Analysis of Breakthrough Invasive Aspergillosis on Anti-Mold Azole Prophylaxis and Treatment: 30-Year Experience in Hematologic Malignancy Patients.","authors":"Hiba Dagher, Anne-Marie Chaftari, Andrea Haddad, Ying Jiang, Jishna Shrestha, Robin Sherchan, Peter Lamie, Jennifer Makhoul, Patrick Chaftari, Ray Hachem, Issam Raad","doi":"10.3390/jof11020160","DOIUrl":"10.3390/jof11020160","url":null,"abstract":"<p><strong>Background: </strong>Anti-mold azoles have improved the outcomes of invasive aspergillosis (IA) when used therapeutically, but they are extensively used as prophylaxis. There are limited data regarding the outcomes of patients with hematologic malignancy who develop breakthrough IA on anti-mold azoles. We aimed to determine whether breakthrough IA on azole prophylaxis shows worse outcomes compared to no prophylaxis.</p><p><strong>Methods: </strong>We compared outcomes including therapy response and mortality between antifungal regimens in hematologic malignancy patients with IA between July 1993 and July 2023.</p><p><strong>Results: </strong>Compared to an amphotericin B-containing regimen (AMB), an anti-mold azole as the primary therapy was independently associated with successful response at the end of therapy (OR = 4.38, <i>p</i> < 0.0001), protective against 42-day IA-associated mortality (OR = 0.51, <i>p</i> = 0.024) or all cause mortality (OR = 0.35, <i>p</i> < 0.0001), and protective against 84-day mortality, both IA-associated (OR = 0.50, <i>p</i> = 0.01) and all-cause mortality (OR = 0.27, <i>p</i> < 0.0001). Azole prophylaxis was independently associated with higher IA-associated mortality at 42 days (OR = 1.91, <i>p</i> = 0.012) and 84 days (OR = 2.03, <i>p</i> = 0.004), compared to fluconazole or no prophylaxis.</p><p><strong>Conclusions: </strong>Patients with breakthrough IA on anti-mold azole prophylaxis show a worse prognosis than those on other or no prophylaxis, possibly related to the emergence of azole resistance due to their widespread use as prophylaxis agents. On the other hand, anti-mold azole primary therapy is superior to AMB therapy in the treatment of IA.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal Policy and Practice Across Five Countries: A Qualitative Review.","authors":"David W Denning, John R Perfect, Neda Milevska-Kostova, Artes Haderi, Hannah Armstrong, Maarten C Hardenberg, Emily Chavez, Bruce Altevogt, Patrick Holmes, Jalal A Aram","doi":"10.3390/jof11020162","DOIUrl":"10.3390/jof11020162","url":null,"abstract":"<p><p>The burden of invasive fungal infections (IFIs) is increasing worldwide. National, regional, and local policies on IFI management should respond to the changing landscape. We assessed antifungal policies from five countries of varying size, IFI burden, and geography: the Netherlands, Italy, South Korea, China, and India. These countries were selected as a representative sample reflecting different types of economic and health systems that patients and providers access worldwide. This assessment focused on a comprehensive range of antifungal policy elements, including recognition and prioritization, awareness and education, prevention and monitoring, diagnosis and coordinated care, access to appropriate treatment, and diagnostic and treatment innovation. Although countries in this analysis all have some form of policy for IFI management, we have identified substantial gaps, including low prioritization of IFI diagnostics, omission of fungal pathogens from antimicrobial resistance policies, and a general lack of awareness and healthcare professional (HCP) training on IFI management. The gaps identified are intended to inform HCPs and policy- and decision-makers about aspects to consider in reducing the IFI burden for patients and health systems while demonstrating responsible antifungal stewardship.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of Antagonistic <i>Trichoderma</i> Strains to Enhance Soybean Growth.","authors":"Na Yu, Yijia Gao, Feng Chang, Wenting Liu, Changhong Guo, Hongsheng Cai","doi":"10.3390/jof11020159","DOIUrl":"10.3390/jof11020159","url":null,"abstract":"<p><p>This study investigates the isolation and screening of <i>Trichoderma</i> strains that exhibit antagonistic properties against soybean root-infecting <i>Fusarium</i> species, particularly <i>F. oxysporum</i>. From soybean rhizosphere soil, 37 antagonistic <i>Trichoderma</i> strains were identified using the plate confrontation method, demonstrating inhibitory effects ranging from 47.57% to 72.86% against <i>F. oxysporum</i>. Strain 235T4 exhibited the highest inhibition rate at 72.86%. Molecular identification confirmed that the strains belonged to eight species within the <i>Trichoderma</i> genus, with notable strains promoting soybean growth in greenhouse tests. In pot experiments, the application of <i>Trichoderma</i> significantly reduced the disease index of soybean plants inoculated with <i>F. oxysporum</i>, particularly with strain 223H16, which achieved an 83.78% control efficiency. Field applications further indicated enhanced soybean growth metrics, including increased pod numbers and plant height, when treated with specific <i>Trichoderma</i> strains. Additionally, <i>Trichoderma</i> application enriched the fungal diversity in the soybean rhizosphere, resulting in a significant reduction of <i>Fusarium</i> populations by approximately 50%. This study highlights the potential of <i>Trichoderma</i> species as biological control agents to enhance soybean health and productivity while improving soil fungal diversity.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856567/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Galleria mellonella</i> as an Invertebrate Model for Studying Fungal Infections.","authors":"Gabriel Davi Marena, Luciana Thomaz, Joshua Daniel Nosanchuk, Carlos Pelleschi Taborda","doi":"10.3390/jof11020157","DOIUrl":"10.3390/jof11020157","url":null,"abstract":"<p><p>The incidence of fungal infections continues to increase and one of the factors responsible for these high rates is the emergence of multi-resistant species, hospitalizations, inappropriate or prolonged use of medications, and pandemics, such as the ongoing HIV/AIDS pandemic. The recent pandemic caused by the severe acute respiratory syndrome virus (SARS-CoV-2) has led to a significant increase in fungal infections, especially systemic mycoses caused by opportunistic fungi. There is a growing and urgent need to better understand how these microorganisms cause infection and develop resistance as well as to develop new therapeutic strategies to combat the diverse diseases caused by fungi. Non-mammalian hosts are increasingly used as alternative models to study microbial infections. Due to their low cost, simplicity of care, conserved innate immunity and reduced ethical issues, the greater wax moth <i>Galleria mellonella</i> is an excellent model host for studying fungal infections and it is currently widely used to study fungal pathogenesis and develop innovative strategies to mitigate the mycoses studied. <i>G. mellonella</i> can grow at 37 °C, which is similar to the mammalian temperature, and the anatomy of the larvae allows researchers to easily deliver pathogens, biological products, compounds and drugs. The aim of this review is to describe how <i>G. mellonella</i> is being used as a model system to study fungal infections as well as the importance of this model in evaluating the antifungal profile of potential drug candidates or new therapies against fungi.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal-Induced Hemophagocytic Lymphohistiocytosis: A Literature Review in Non-HIV Populations.","authors":"Chia-Yu Chiu, Rachel S Hicklen, Dimitrios P Kontoyiannis","doi":"10.3390/jof11020158","DOIUrl":"10.3390/jof11020158","url":null,"abstract":"<p><p>We performed a thorough search of the literature published through December 2024 with no date exclusions on invasive fungal infection (IFI)-induced hemophagocytic lymphohistiocytosis (HLH) in non-human immunodeficiency virus (HIV) patients. The frequency of IFI-induced HLH reported across 16 articles was 9%. Of the 116 identified cases with available clinical information, 53% occurred in immunocompromised patients. IFIs were usually disseminated (76%), with <i>Histoplasma capsulatum</i> being the most common pathogen (51%). IFI and HLH were diagnosed simultaneously in most cases (78%). The 30-day survival rate was 64%. Reported cases had significant heterogeneity in patient characteristics, management strategies, and outcomes.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species Diversity and Community Composition of Macrofungi in the Dongling Mountains, Western Beijing, China.","authors":"Jia-Mao Huang, Tai-Min Xu, Wen Zhao, Reyila Mumin, Long Zeng, Yi-Fei Sun, Bao-Kai Cui","doi":"10.3390/jof11020155","DOIUrl":"10.3390/jof11020155","url":null,"abstract":"<p><p>Macrofungi are a group of fungi with important ecological functions and economic value in forest ecosystems. In this study, the species diversity and community composition of macrofungi in different areas, vegetation types, and elevation gradients from the Lingshan Natural Scenic Area and Xiaolongmen National Forest Park of the Dongling Mountains, Western Beijing were investigated. A total of 1379 specimens were collected. All specimens were identified by morphological and molecular methods, resulting in the identification of 311 species belonging to two phyla, four classes, 18 orders, 74 families, and 147 genera. The alpha diversity analysis indicated that the alpha diversity was significantly different among different vegetation types. The <i>Betula</i> spp. and <i>Populus davidiana</i> of the Lingshan Natural Scenic Area, and <i>Quercus liaotungensis</i> of Xiaolongmen National Forest Park had higher macrofungal alpha diversity. The difference in alpha diversity between the two areas was not significant. The principal coordinate analysis indicated a significant difference in macrofungal community composition among different vegetation types. The fungal community composition of the two areas was also significantly different. The fungal species richness and unique species tended to increase and then decrease with increasing elevation. The species composition of neighboring elevation gradients was more similar. The macrofungal species richness and the number of unique species were not significantly affected by elevation gradient. The vegetation types with higher fungal alpha diversity in the Dongling Mountains were <i>Betula</i> spp., <i>Populus davidiana</i>, and <i>Quercus liaotungensis</i>, and there were abundant fungal species in deciduous broad-leaved mixed forests. Macrofungal diversity and community composition was significantly affected by vegetation type. To better protect the macrofungi, the protection of these four vegetation types in the Dongling Mountains should be strengthened in the future.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856809/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}