Journal of Fungi最新文献

{"title":"<i>Colletotrichum gloeosporioides</i> Swiftly Manipulates the Transcriptional Regulation in <i>Citrus sinensis</i> During the Early Infection Stage.","authors":"Siyu Zhang, Xinyou Wang, Wei Zeng, Leijian Zhong, Xiaoyong Yuan, Zhigang Ouyang, Ruimin Li","doi":"10.3390/jof10110805","DOIUrl":"10.3390/jof10110805","url":null,"abstract":"<p><p><i>Citrus</i> spp. represent an economically important fruit tree crop worldwide. However, molecular mechanisms underlying the interaction between citrus and the <i>Colletotrichum gloeosporioides</i> remain largely unexplored. In this study, we analyzed the physiological and transcriptomic changes in <i>Citrus sinensis</i> at different stages of incubation with <i>C. gloeosporioides</i>. The results indicated that <i>C. gloeosporioides</i> infection rapidly triggered necrosis in the epicarp of <i>C. sinensis</i> fruits, decreased the total flavonoid contents, and suppressed the activity of catalase, peroxidase, and superoxide dismutase enzymes. Upon inoculation with <i>C. gloeosporioides</i>, there were 4600 differentially expressed genes (DEGs) with 1754 down-regulated and 2846 up-regulated after six hours, while there were only 580 DEGs with 185 down-regulated and 395 up-regulated between six and twelve-hours post-inoculation. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that the DEGs, which exhibited consistent up-regulation, were associated with metabolic processes and stress responses. Through Weighted Gene Co-Expression Network Analysis, 11 key genes have been identified that could potentially play a role in the transcriptional regulation of this process, including the transcription factor bHLH189. Furthermore, the infection of <i>C. gloeosporioides</i> had a notable effect on both the flavonoid metabolism and the metabolic pathways related to reactive oxygen species. Our findings help to understand the interaction between citrus and <i>C. gloeosporioides</i> and unveil how new insights into how <i>C. gloeosporioides</i> circumvents citrus defense mechanisms.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Two <i>Trichoderma</i> Strains on Apple Replant Disease Suppression and Plant Growth Stimulation.","authors":"Wen Du, Pengbo Dai, Mingyi Zhang, Guangzhu Yang, Wenjing Huang, Kuijing Liang, Bo Li, Keqiang Cao, Tongle Hu, Yanan Wang, Xianglong Meng, Shutong Wang","doi":"10.3390/jof10110804","DOIUrl":"10.3390/jof10110804","url":null,"abstract":"<p><p><i>Fusarium oxysporum</i>, the pathogen responsible for apple replant disease (ARD), is seriously threatening the apple industry globally. We investigated the antagonistic properties of <i>Trichoderma</i> strains against <i>F. oxysporum</i> HS2, aiming to find a biological control solution to minimize the dependence on chemical pesticides. Two of the thirty-one <i>Trichoderma</i> strains assessed through plate confrontation assays, L7 (<i>Trichoderma atroviride</i>) and M19 (<i>T. longibrachiatum</i>), markedly inhibited = <i>F. oxysporum</i>, with inhibition rates of 86.02% and 86.72%, respectively. Applying 1 × 10⁶ spores/mL suspensions of these strains notably increased the disease resistance in embryonic mung bean roots. Strains L7 and M19 substantially protected <i>Malus robusta</i> Rehd apple rootstock from ARD; the plant height, stem diameter, leaf number, chlorophyll content, and defense enzyme activity were higher in the treated plants than in the controls in both greenhouse and field trials. The results of fluorescent labeling confirmed the effective colonization of these strains of the root soil, with the number of spores stabilizing over time. At 56 days after inoculation, the M19 and L7 spore counts in various soils confirmed their persistence. These results underscore the biocontrol potential of L7 and M19 against HS2, offering valuable insights into developing sustainable ARD management practices.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of <i>Diaporthe goulteri</i> on Soybean in Germany.","authors":"Behnoush Hosseini, Maximilian Gerhard Gröbner, Tobias Immanuel Link","doi":"10.3390/jof10110803","DOIUrl":"10.3390/jof10110803","url":null,"abstract":"<p><p><i>Diaporthe</i> (anamorph: <i>Phomopsis</i>) species are endophytes or fungal pathogens for many different plant species. Soybean (<i>Glycine max</i>) can be infected by many different <i>Diaporthe</i> species; among them, <i>D. caulivora</i> and <i>D. longicolla</i> are responsible for the most significant damages. <i>Diaporthe goulteri</i> is a species that was only recently described and has so far been found on sunflower (<i>Helianthus annuus</i>) in Australia and an unknown host in Thailand. Here, we report isolation of <i>D. goulteri</i> from soybean in southern Germany, molecular species identification, and additional morphological description. We also show that <i>D. goulteri</i> can infect soybean and describe the symptoms we observed, both on the plant where the isolate came from and following artificial inoculation.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Expanding Truffle Environment: A Study of the Microbial Dynamics in the Old Productive Site and the New <i>Tuber magnatum</i> Picco Habitat.","authors":"Mara Rondolini, Maurizio Zotti, Gilberto Bragato, Leonardo Baciarelli Falini, Lara Reale, Domizia Donnini","doi":"10.3390/jof10110800","DOIUrl":"10.3390/jof10110800","url":null,"abstract":"<p><p>Truffles are valuable underground mushrooms with significant economic importance. In recent years, their cultivation has achieved satisfactory results, but not for all species. The harvesting of white truffles (<i>Tuber magnatum</i> Picco) is still dependent on natural production, which is at risk due to various issues, such as improper forest management. A useful practice to protect natural resources is to promote the expansion of productive forests. In this study, we investigate the dynamics of the microbiome in an old and new truffle forest using an amplicon sequencing approach of the fungal ITS region and the prokaryotic 16S rRNA gene. We monitor the soil biological community's development to compare differences and similarities between the primary productive forest and the expanding area over a two-year sampling period. In particular, we observed the colonization of vacant ecological niches by certain fungi, such as those belonging to the genus <i>Mortierella</i>. Additionally, we examined the competitive interactions between saprotrophs and ectomycorrhizal fungi (ECM). In both study areas, the bacterial community was dominated by Pseudomonadota, Planctomycetota, and Actinomycetota. The behavior of the <i>Tuber</i> genus differed significantly from other ECMs and displayed positive correlations with bacterial taxa such as <i>Ktedonobacter</i>, <i>Zavarzinella</i>, and <i>Sphingomonas</i>. The present work provides an initial overview of expanding white truffle habitats. Further, more specific research is needed to explore potential connections between individual <i>taxa</i>.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous L-Arginine Enhances Pathogenicity of <i>Alternaria alternata</i> on Kiwifruit by Regulating Metabolisms of Nitric Oxide, Polyamines, Reactive Oxygen Species (ROS), and Cell Wall Modification.","authors":"Di Wang, Lingkui Meng, Haijue Zhang, Rong Liu, Yuhan Zhu, Xinyu Tan, Yan Wu, Qingchao Gao, Xueyan Ren, Qingjun Kong","doi":"10.3390/jof10110801","DOIUrl":"10.3390/jof10110801","url":null,"abstract":"<p><p>Black spot, one of the major diseases of kiwifruit, is caused by <i>Alternaria alternata</i>. A comprehensive investigation into its pathogenicity mechanism is imperative in order to propose a targeted and effective control strategy. The effect of L-arginine on the pathogenicity of <i>A. alternata</i> and the underlying mechanisms were investigated. The results showed that treatment with 5 mM L^-1 of L-arginine promoted spore germination and increased the colony diameter and lesion diameter of <i>A. alternata</i> in vivo and in vitro, which were 23.1% and 9.3% higher than that of the control, respectively. Exogenous L-arginine treatment also induced endogenous L-arginine and nitric oxide (NO) accumulation by activating nitric oxide synthase (NOS), arginine decarboxylase (ADC) and ornithine decarboxylase (ODC). In addition, exogenous L-arginine triggered an increase in reactive oxygen species (ROS) levels by activating the activity and inducing gene expression upregulation of NADPH oxidase. The hydrogen peroxide (H₂O₂) and superoxide anion (O₂^.-) levels were 15.9% and 2.2 times higher, respectively, than in the control group on the second day of L-arginine treatment. Meanwhile, antioxidant enzyme activities and gene expression levels were enhanced, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase (GPX), and glutathione reductase (GR). In addition, exogenous L-arginine stimulated cell wall-degrading enzymes in vivo and in vitro by activating gene expression. These results suggested that exogenous L-arginine promoted the pathogenicity of <i>A. alternata</i> by inducing the accumulation of polyamines, NO, and ROS, and by activating systems of antioxidants and cell wall-degrading enzymes. The present study not only revealed the mechanism by which low concentrations of L-arginine increase the pathogenicity of <i>A. alternata</i>, but also provided a theoretical basis for the exclusive and precise targeting of <i>A. alternata</i> in kiwifruit.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595605/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population Structure and Mating Type Distribution of <i>Cercospora sojina</i> from Soybeans in Indiana, United States.","authors":"Guohong Cai, Leandro Lopes da Silva, Natalia Piñeros-Guerrero, Darcy E P Telenko","doi":"10.3390/jof10110802","DOIUrl":"10.3390/jof10110802","url":null,"abstract":"<p><p>Frogeye leaf spot on soybeans is traditionally considered as a southern disease in the United States but its impact in North Central USA has been rising in recent years. In this study, we investigated the population structure and mating type distribution in the <i>C. sojina</i> population from Indiana, USA. Based on 27 single nucleotide polymorphism markers, 49 multi-locus genotypes (MLGs) were identified in 234 isolates collected from 29 counties in Indiana in 2020. Bayesian analysis grouped the 49 MLGs into three clusters. This grouping was supported by principal coordinate analysis and, in large part, by the unweighted pair group method with arithmetic mean and minimal spanning tree. Only one mating-type idiomorph was found in each isolate and in each MLG. The <i>MAT1-1</i> idiomorph was found in 22 MLGs and the <i>MAT1-2</i> idiomorph was found in 27 MLGs. Based on clone-corrected data, the distribution of mating-type idiomorphs did not deviate significantly from 1:1 ratio in Indiana as a whole and in 22 out of 24 counties where two or more MLGs were found. Thirty MLGs contained QoI-resistant isolates and 22 MLGs contained QoI-sensitive isolates, with three MLGs containing both types of isolates. MLG1, the most common MLG with 90 isolates, contained mostly QoI-resistant isolates. Interestingly, MLG1 was also the dominant genotype in the Tennessee population collected in 2015, suggesting that MLG1 has been a dominant genotype in a wider region for many years. Based on the standard index of association (r¯_d), the Indiana population as a whole was in significant linkage disequilibrium. However, in five out of 16 counties where three or more MLGs were found, the null hypothesis of linkage equilibrium was not rejected. Tests of linkage disequilibrium between locus pairs showed that 33.3% of locus pairs on the same contigs were in significant disequilibrium and 17.7% of locus pairs on different contigs were in significant disequilibrium. The possibility of a cryptic sexual stage was discussed.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and Characterization of <i>Colletotrichum</i> Species Associated with Maize in Sichuan, China.","authors":"Rui Yang, Ying Li, Henan Zhao, Xiaofang Sun, Wen Chen, Pan Li, Xuehu Li, Cuiping Wu, Miaomiao Ma, Guoshu Gong","doi":"10.3390/jof10110799","DOIUrl":"10.3390/jof10110799","url":null,"abstract":"<p><p><i>Colletotrichum</i> species are the most common cause of maize anthracnose, which often manifests as leaf spots. However, field observations often reveal symptoms similar to those caused by other leaf spot pathogens, such as <i>Curvularia</i> and <i>Bipolaris</i>. In this study, 99 isolates were identified using tissue separation and single-spore isolation techniques. As preliminary measures of species diversity, all 99 isolates were identified morphologically, and the glyceraldehyde-3-phosphate dehydrogenase (<i>GAPDH</i>) gene sequences were phylogenetically analyzed. Furthermore, 48 representative strains were selected for molecular identification using multi-locus phylogenetic analyses based on five gene loci (ITS, <i>TUB</i>, <i>ACT</i>, <i>GAPDH</i>, and <i>CAL</i>). Finally, 10 species of <i>Colletotrichum</i> isolated from maize leaf spots were identified. <i>Colletotrichum cliviicola</i> was the most dominant species (24.2%), followed by <i>C. fructicola</i> (18.2%), <i>C. karstii</i> (16.1%), <i>C. siamense</i> (13.1%), <i>C. boninense</i> (7.1%), <i>C. kahawae</i> (7.1%), <i>C. brevisporum</i> (6.1%), <i>C. truncatum</i> (5.1%), <i>C. gigasporum</i> (2.0%), and <i>C. gloeosporioides</i> (1.0%). For the first time, pathogenicity tests revealed that <i>C. cliviicola</i>, <i>C. fructicola</i>, <i>C. siamense</i>, <i>C. karstii</i>, and <i>C. truncatum</i> are the causative agents of maize anthracnose. Additionally, <i>C. boninense</i> was identified as an endophytic fungus on healthy maize. In conclusion, this study is the first to identify the pathogen of maize anthracnose in Sichuan Province. It provides valuable insights for accurately diagnosing and managing maize anthracnose.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ppn2 Polyphosphatase Improves the Ability of <i>S. cerevisiae</i> to Grow in Mild Alkaline Medium.","authors":"Irina A Eliseeva, Lubov Ryazanova, Larisa Ledova, Anton Zvonarev, Airat Valiakhmetov, Maria Suntsova, Aleksander Modestov, Anton Buzdin, Dmitry N Lyabin, Ivan V Kulakovskiy, Tatiana Kulakovskaya","doi":"10.3390/jof10110797","DOIUrl":"10.3390/jof10110797","url":null,"abstract":"<p><p>Inorganic polyphosphates and respective metabolic pathways and enzymes are important factors for yeast active growth in unfavorable conditions. However, particular proteins of polyphosphate metabolism remain poorly explored in this context. Here we report biochemical and transcriptomic characterization of the CRN/PPN2 yeast strain (derived from Ppn1-lacking CRN strain) overexpressing poorly studied Ppn2 polyphosphatase. We showed that Ppn2 overexpression significantly reduced lag phase in the alkaline medium presumably due to the ability of Ppn2 to efficiently hydrolyze inorganic polyphosphates and thus neutralize hydroxide ions in the cell. With RNA-Seq, we compared the molecular phenotypes of CRN/PPN2 and its parent CRN strain grown in YPD or alkaline medium and detected transcriptomic changes induced by Ppn2 overexpression and reflecting the adaptation to alkaline conditions. The core set of upregulated genes included several genes with a previously unknown function. Respective knockout strains (∆<i>ecm8</i>, ∆<i>yol160w</i>, ∆<i>cpp3</i>, ∆<i>ycr099c</i>) exhibited defects of growth or cell morphology in the alkaline medium, proving the functional involvement of the respective proteins in sustaining growth in alkaline conditions.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular Biosynthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Synthesized by Filamentous Fungi.","authors":"Iuliana Răut, Mariana Constantin, Raluca Șuică-Bunghez, Cristina Firincă, Elvira Alexandrescu, Ioana Cătălina Gîfu, Mihaela Doni, Lucian-Gabriel Zamfir, Ana-Maria Gurban, Luiza Jecu","doi":"10.3390/jof10110798","DOIUrl":"10.3390/jof10110798","url":null,"abstract":"<p><p>The green synthesis of metal nanoparticles has received substantial attention due to their applications in various domains. The aim of the study was to obtain silver nanoparticles (AgNPs) by green synthesis with filamentous fungi, such as <i>Cladosporium cladosporoides</i>, <i>Penicillium chrysogenum</i>, and <i>Purpureocillium lilacinum</i>. Fungal species were grown on nutrient media and aqueous mycelium extracts were used to reduce Ag⁺ to Ag (0). The silver nanoparticles were analyzed by various techniques, such as UV-Visible spectroscopy (UV-Vis), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), dynamic light scattering (DLS), and Zeta potential. The formation of silver nanoparticles was confirmed by UV-Vis spectroscopy and the color change of the mixture containing metal precursor and aqueous mycelium extract. FTIR displayed different functional groups as capping and reducing agents for the biosynthesis of AgNPs. SEM and TEM provided information on the particles' morphology. DLS diagrams indicated mean particle diameters in the 124-168 nm region. All biosynthesized AgNPs had negative zeta values, which is a sign of good stability. Silver nanoparticles were evaluated for antimicrobial activity, and the most active were those synthesized with metabolites from <i>Cladosporium</i>, leading to 93.75% inhibition of <i>Staphylococcus aureus</i>, 67.20% of <i>Escherichia coli</i>, and 69.56% of <i>Candida albicans</i>. With the highest microbial inhibition percentage and a very good Poly Dispersion Index (Pd I), <i>Cladosporium cladosporoides</i> was selected as an environmentally friendly source of silver nanoparticles that could be used as a potential antimicrobial agent.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of the Endophytic Microbiome in <i>Cotinus coggygria</i> Roots to Verticillium Wilt Infection.","authors":"Yanli Cheng, Juan Zhao, Yayong Liu, Taotao Zhang, Tom Hsiang, Zhihe Yu, Wentao Qin","doi":"10.3390/jof10110792","DOIUrl":"10.3390/jof10110792","url":null,"abstract":"<p><p>Verticillium wilt caused by <i>Verticillium dahliae</i> Kleb. is a lethal soil-borne fungal disease of <i>Cotinus coggygria</i>. The plant endophytic microbiome plays an important role in maintaining plant health and disease resistance, but it is unclear how the endophytic microbiome of <i>C. coggygria</i> roots varies in response to Verticillium wilt occurrence. In this study, the endophytic microbial diversity, community composition, dominant species, and co-occurrence network of <i>C. coggygria</i> under Verticillium wilt-affected and healthy conditions were assessed using Illumina sequencing. Compared with healthy plants, the bacterial alpha diversity indices of Verticillium wilt-affected plants decreased significantly, while the fungal alpha diversity indices showed obvious increases. The relative abundance of dominant taxa including Proteobacteria, Actinobacteriota, Ascomycota, and Basidiomycota at the phylum level, as well as Gammaproteobacteria, Thermoleophilia, Dothideomycetes, and Agaricomycetes at the class level, differed significantly between Verticillium wilt-affected and healthy plants. Co-occurrence networks revealed that the fungal network of Verticillium wilt-affected roots was denser with more negative interactions, which may be relevant to functional changes from reciprocity to competition in the microbial community, in response to <i>V. dahliae</i> infection. The results enhanced our understanding on the relationships between the endophytic microbiome and Verticillium wilt, which could provide information for the management of this disease in <i>C. coggygria</i>.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595824/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}