Journal of Fungi最新文献_第2页

Culturable Yeast Diversity Associated with Industrial Cultures of the Microalga Microchloropsis gaditana and Their Ability to Produce Lipids and Biosurfactants.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-03-17 DOI: 10.3390/jof11030228

Madalena Matos, Mónica A Fernandes, Inês Costa, Natacha Coelho, Tamára F Santos, Veronica Rossetto, João Varela, Isabel Sá-Correia

{"title":"Culturable Yeast Diversity Associated with Industrial Cultures of the Microalga Microchloropsis gaditana and Their Ability to Produce Lipids and Biosurfactants.","authors":"Madalena Matos, Mónica A Fernandes, Inês Costa, Natacha Coelho, Tamára F Santos, Veronica Rossetto, João Varela, Isabel Sá-Correia","doi":"10.3390/jof11030228","DOIUrl":"10.3390/jof11030228","url":null,"abstract":"The marine oleaginous microalga Microchloropsis gaditana (formerly Nannochloropsis gaditana) exhibits a high capacity to thrive in a broad range of environmental conditions, being predominantly utilized as feed in aquaculture. This article reports the characterization of the culturable yeast population present during the scale-up process of M. gaditana cultivation at Necton S.A. facilities, from 5 L flasks until tubular photobioreactors. The 146 yeast isolates obtained, molecularly identified based on D1/D2 and ITS nucleotide sequences, belong to the species Rhodotorula diobovata, R. mucilaginosa, R. taiwanensis, R. sphaerocarpa, Vishniacozyma carnescens, Moesziomyces aphidis, and Meyerozyma guilliermondii. The yeast abundance was found to increase throughout upscaling stages. The yeast populations isolated from microalgal cultures and water samples share phylogenetically close isolates, indicating a possible common source. The impressive high percentage of red yeasts isolated (90%) is consistent with the recognized role of carotenoid pigments in yeast photoprotection. Sixty yeast isolates were tested for lipid (Nile Red staining) and biosurfactant (oil drop dispersion and emulsification index) production. Results revealed that these capacities are common features. Microbial lipids and biosurfactants have promising biotechnological applications. Moreover, biosurfactants can fulfill various physiological roles and provide advantages in natural environments contributing to the promising use of yeasts as probiotics in microalgae production.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943003/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome Sequencing and Comparative Transcriptomic Analysis of Rice Brown Spot Pathogen Bipolaris oryzae Adaptation to Osmotic Stress.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-03-17 DOI: 10.3390/jof11030227

Chun Wang, Kexin Yang, Sauban Musa Jibril, Ruoping Wang, Chengyun Li, Yi Wang

{"title":"Genome Sequencing and Comparative Transcriptomic Analysis of Rice Brown Spot Pathogen Bipolaris oryzae Adaptation to Osmotic Stress.","authors":"Chun Wang, Kexin Yang, Sauban Musa Jibril, Ruoping Wang, Chengyun Li, Yi Wang","doi":"10.3390/jof11030227","DOIUrl":"10.3390/jof11030227","url":null,"abstract":"Rice brown spot disease, caused by Bipolaris oryzae, is a significant fungal disease that poses a major threat to global rice production. Despite its widespread impact, genomic studies of B. oryzae remain limited, particularly those involving high-quality genomic data. In this study, we performed whole-genome sequencing of the B. oryzae strain RBD1, which was isolated from the demonstration field for upland rice cultivation in Haozhiba Village, Lancang County, Pu'er City, Yunnan Province, China, using a combination of second-generation Illumina sequencing and third-generation Single-Molecule Real-Time (SMRT) sequencing. The assembled genome was 37.5 Mb in size with a G + C content of 49.39%, containing 42 contigs with a contig N50 of 2.0 Mb. Genomic analysis identified genes related to carbon, nitrogen, and lipid metabolism, highlighting the strain's metabolic flexibility under diverse environmental conditions and host interactions. Additionally, we identified pathogenicity-related genes involved in MAPK signaling, G protein signaling, and oxidative stress responses. Under 1.2 M sorbitol-induced osmotic stress, we observed significant differences in growth responses between RBD1 and the rice blast fungus Magnaporthe oryzae H7. Transcriptomic analysis using Illumina sequencing revealed that RBD1 responds to osmotic stress by enhancing carbohydrate metabolism, fatty acid degradation, and amino acid synthesis, while H7 primarily relies on protein synthesis to enhance growth tolerance. This study provides a valuable foundation for understanding the pathogenic mechanisms of rice brown spot and future disease control strategies.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943334/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A New Root and Trunk Rot Disease of Grapevine Plantlets Caused by Fusarium in Four Species Complexes.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-03-17 DOI: 10.3390/jof11030230

José Manoel Ferreira de Lima Cruz, Otília Ricardo de Farias, Brunno Cassiano Lemos Araújo, Alejandra Valencia Rivera, Cláudia Rita de Souza, Jorge Teodoro de Souza

{"title":"A New Root and Trunk Rot Disease of Grapevine Plantlets Caused by Fusarium in Four Species Complexes.","authors":"José Manoel Ferreira de Lima Cruz, Otília Ricardo de Farias, Brunno Cassiano Lemos Araújo, Alejandra Valencia Rivera, Cláudia Rita de Souza, Jorge Teodoro de Souza","doi":"10.3390/jof11030230","DOIUrl":"10.3390/jof11030230","url":null,"abstract":"Grapevines are propagated by grafting, but the rootstocks used in commercial plantations are susceptible to several diseases. In this study, we focused on a novel root and trunk rot disease of grapevine plantlets that show symptoms during cold storage, before field establishment. Our objectives were to study the aetiology, symptomatology, plant resistance responses, and mode of action of the pathogen that was initially identified as Fusarium. The characterisation of this pathosystem was performed by isolation, pathogenicity assays, genetic diversity studies with BOX-PCR, and identification by sequencing a fragment of the tef1 gene. Scanning electron microscopy and X-ray spectroscopy were used to study the mode of action and plant resistance responses. The results showed that 12 species of Fusarium, initially isolated from both healthy and diseased plantlets, and classified into 4 species complexes, were pathogenic to grapevines. Comparative analyses between diseased and healthy roots showed typical resistance responses in diseased plantlets, including tyloses formation, translocation of Ca, and accumulation of Si. Field experiments confirmed that 100% of the diseased plantlets died within 90 days of transplantation. This study contributes to a better understanding of root and trunk rot disease under cold storage and provides insights for the development of management strategies.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcription Factors in Biocontrol Fungi.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-03-15 DOI: 10.3390/jof11030223

Han-Jian Song, Xiao-Feng Li, Xin-Ran Pei, Zhan-Bin Sun, Han-Xu Pan

{"title":"Transcription Factors in Biocontrol Fungi.","authors":"Han-Jian Song, Xiao-Feng Li, Xin-Ran Pei, Zhan-Bin Sun, Han-Xu Pan","doi":"10.3390/jof11030223","DOIUrl":"10.3390/jof11030223","url":null,"abstract":"Transcription factors are extensively found in fungi and are involved in the regulation of multiple biological processes, including growth, development, conidiation, morphology, stresses tolerance, and virulence, as well as the production of secondary metabolites. Biocontrol is a complex biological process through which several biocontrol behaviors, such as the secretion of cell wall-degrading enzymes and the production of secondary metabolites, are regulated by transcription factors. To date, biocontrol-related transcription factors have been reported in several biocontrol fungi, such as Beauveria bassiana, Clonostachys rosea, Coniothyrium minitans, and different species in the genera Metarhizium, Trichoderma, and Arthrobotrys. However, comprehensive reviews summarizing and analyzing transcription factors with biocontrol potential in these fungi are scarce. This review begins by giving a basic overview of transcription factors and their functions. Then, the role of biocontrol-related transcription factors in biocontrol fungi is discussed. Lastly, possible approaches for further work on transcription factors in biocontrol fungi are suggested. This review provides a basis for further elaborating the molecular mechanisms of transcription factors in the context of biocontrol.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of Alternaria Section Nimbya in Iran, with the Description of Eight New Species.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-03-15 DOI: 10.3390/jof11030225

Abdollah Ahmadpour, Youbert Ghosta, Zahra Alavi, Fatemeh Alavi, Alireza Poursafar, Pabulo Henrique Rampelotto

{"title":"Diversity of Alternaria Section Nimbya in Iran, with the Description of Eight New Species.","authors":"Abdollah Ahmadpour, Youbert Ghosta, Zahra Alavi, Fatemeh Alavi, Alireza Poursafar, Pabulo Henrique Rampelotto","doi":"10.3390/jof11030225","DOIUrl":"10.3390/jof11030225","url":null,"abstract":"Alternaria includes endophytes, saprophytes, and pathogens affecting both plants and animals, with a global distribution across various hosts and substrates. It is categorized into 29 sections, each defined by a type species and six monophyletic lineages. The Alternaria section Nimbya comprises 10 species primarily associated with the families Juncaceae and Cyperaceae, functioning as either saprophytes or plant pathogens. In this study, 189 fungal strains were collected from multiple locations across six provinces in Iran. The isolates were initially classified based on morphological characteristics and ISSR-PCR molecular marker banding patterns. Multi-gene phylogenetic analyses of 38 selected strains, using ITS-rDNA, GAPDH, TEF1, RPB2, and Alt a 1 gene sequences, combined with morphological data, led to the identification of 13 species, including eight new species, namely Alternaria caricifolia, A. cyperi, A. juncigena, A. junci-inflexi, A. persica, A. schoenoplecti, A. salkadehensis, and A. urmiana. In addition, this work identified new host associations (matrix nova) for three previously known species: A. caricicola on Cyperus sp., A. cypericola on Eleocharis sp., and A. junci-acuti on Carex sp. The study provides detailed morphological descriptions and illustrations of all identified species, discusses their habitats, distribution, and phylogenetic relationships within section Nimbya, and presents a key for species identification within this section in Iran. Furthermore, these findings highlight the significance of studying fungal biodiversity in Iran and contribute to a better understanding of species distribution and host range within the Alternaria section Nimbya.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Serum Biomarkers for Improved Diagnosis of Candidemia.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-03-15 DOI: 10.3390/jof11030224

Amélie Kinet-Poleur, Corentin Deckers, Imane Saad Albichr, Pierre Bogaerts, Patrick M Honoré, Pierre Bulpa, Nathalie Ausselet, Frederic Foret, François Kidd, Te-Din Huang, Isabel Montesinos

{"title":"Evaluation of Serum Biomarkers for Improved Diagnosis of Candidemia.","authors":"Amélie Kinet-Poleur, Corentin Deckers, Imane Saad Albichr, Pierre Bogaerts, Patrick M Honoré, Pierre Bulpa, Nathalie Ausselet, Frederic Foret, François Kidd, Te-Din Huang, Isabel Montesinos","doi":"10.3390/jof11030224","DOIUrl":"10.3390/jof11030224","url":null,"abstract":"Background: Candidemia, associated with high morbidity and mortality, remains challenging to diagnose due to the limitations of blood cultures. Serological biomarkers offer faster detection, enabling earlier treatment and improving outcomes.Methods: This study, conducted at CHU UCL Namur (August 2023-January 2025), evaluated three diagnostic biomarkers for candidemia: CAGTA IgG VirClia Monotest, Wako β-D-glucan test, and CandId OLM RT-PCR. A total of 35 candidemia cases and 20 controls were included.Results: CAGTA IgG VirClia Monotest demonstrated low sensitivity (46%) and moderate specificity (75%). Both Wako β-D-glucan test and CandId OLM RT-PCR exhibited higher sensitivity (74% and 71%, respectively) and excellent specificity (100%). The combined use of Wako β-D-glucan test and CandId OLM RT-PCR further enhanced diagnostic performance, achieving 91% sensitivity and 100% specificity. Misidentification between Candida albicans and Candida dubliniensis was observed with CandId PCR, underlining a potential limitation in species-level discrimination.Conclusions: Both Wako β-D-glucan test and CandID OLM RT-PCR outperformed CAGTA IgG VirClia Monotest in diagnosing candidemia, and their combination significantly improved diagnostic accuracy, albeit at a higher cost. These findings highlight the potential of integrating multiple biomarkers into diagnostic workflows to optimize early detection, though further studies are needed to refine strategies and address challenges.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942904/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fungal-Infected Weeds: A Potential Source of Leaf Spot Disease in Rubber Trees from Southern Thailand.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-03-14 DOI: 10.3390/jof11030220

Narit Thaochan, Chaninun Pornsuriya, Thanunchanok Chairin, Kodeeyah Thoawan, Putarak Chomnunti, Anurag Sunpapao

{"title":"Fungal-Infected Weeds: A Potential Source of Leaf Spot Disease in Rubber Trees from Southern Thailand.","authors":"Narit Thaochan, Chaninun Pornsuriya, Thanunchanok Chairin, Kodeeyah Thoawan, Putarak Chomnunti, Anurag Sunpapao","doi":"10.3390/jof11030220","DOIUrl":"10.3390/jof11030220","url":null,"abstract":"The rubber tree (Hevea brasiliensis) is an economically important crop in Thailand. Severe defoliation caused by emerging diseases has been reported to substantially reduce rubber yields during the leaf fall phase. The classical disease dispersal patterns of fungi in rubber tree plantations might be derived from weeds in adjacent fields. However, this hypothesis remains untested. Therefore, in this study, we collected and isolated fungi from symptomatic weed samples in rubber tree plantations in Krabi Province in southern Thailand. We found that Parameria sp. were dominant, showing the development of conidiomata on leaves. A total of 25 symptomatic Parameria sp. leaves were collected and tested for their pathogenicity on rubber tree leaves. The tests produced six fungal isolates, WC001, WC002, WL001, WL002, WN001, and WN002, that caused spots on the rubber tree leaves similar to those observed on the weeds. Morphological characterization revealed that fungal isolates WC001 and WC002 were Colletotrichum sp., WL001 and WL002 were Lasiodiplodia sp., and WN001 and WN002 were Neopestalotiopsis sp. Multigene phylogenetic analyses of combined act, gapdh, ITS, and tub2 regions identified WC001 and WC002 as Colletotrichum siamense, while analyses of ITS, tub2, and tef1-α regions identified WL001 and WL002 as Lasiodiplodia brasiliensis and WN001 and WN002 as Neopestalotiopsis cubana. The occurrence of fungal diseases in rubber trees is significantly associated with leafy weeds in and around rubber tree plantations that could constitute reservoirs of fungal pathogens. The strategies used to control weeds have to be further considered in the future.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different Proteostasis Mechanisms Facilitate the Assembly of Individual Components on the Chitin Synthase 3 Complex at the Endoplasmic Reticulum.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-03-14 DOI: 10.3390/jof11030221

Noelia Sánchez, Rosario Valle, César Roncero

{"title":"Different Proteostasis Mechanisms Facilitate the Assembly of Individual Components on the Chitin Synthase 3 Complex at the Endoplasmic Reticulum.","authors":"Noelia Sánchez, Rosario Valle, César Roncero","doi":"10.3390/jof11030221","DOIUrl":"10.3390/jof11030221","url":null,"abstract":"Chitin synthase 3 complex assembly begins at the endoplasmic reticulum where the formation of a Chs3/Chs7 complex facilitates its exit from the ER and its transport along the secretory route. In the present study, our work shows that orphan molecules of Chs7 can exit the ER and are later recycled from the early Golgi by coat protein I (COPI) machinery via the adaptor complex Erv41/Erv46. Moreover, an eventual excess of the protein in the Golgi is recognized by the GGA complex and targeted to the vacuole for degradation through the ESCRT machinery. Non-oligomerizable versions of Chs3 can also exit the ER individually and follow a similar route to that of Chs7. We therefore demonstrate the traffic of unassembled CS3 subunits and describe the cellular mechanisms that guarantee the correct assembly of this protein complex at the ER while providing a default traffic route to the vacuole in case of its failure. This traffic route is shared with canonical ER adaptors, such as Erv29 and Erv14, and other components of protein complexes. The comparative analysis of their traffic allows us to discern a cellular program that combines COPI recycling, proteasomal degradation, and vacuolar disposal for maintaining protein homeostasis at the ER.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943272/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variation of Arbuscular Mycorrhizal Fungi Communities Between Root and Rhizosphere Soil of Endangered Plant Heptacodium miconioides Along Elevation Gradient.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-03-14 DOI: 10.3390/jof11030222

Yueling Li, Guangyu Luo, Shijie Wu, Dang Yang, Pengpeng Lv, Zexin Jin, Junmin Li

{"title":"Variation of Arbuscular Mycorrhizal Fungi Communities Between Root and Rhizosphere Soil of Endangered Plant Heptacodium miconioides Along Elevation Gradient.","authors":"Yueling Li, Guangyu Luo, Shijie Wu, Dang Yang, Pengpeng Lv, Zexin Jin, Junmin Li","doi":"10.3390/jof11030222","DOIUrl":"10.3390/jof11030222","url":null,"abstract":"Arbuscular mycorrhizal fungi (AMF) are considered crucial for the survival of many endangered plant species. However, the dynamics of AMF communities in the roots and rhizosphere soil of Heptacodium miconioides, particularly along elevation gradients, remain underexplored. This study investigates AMF colonization, spore density, and community structure in the root and rhizosphere soil of H. miconioides across an elevation range from 306 to 1028 m a.s.l., employing high-throughput sequencing. Our results show that AMF colonization and spore density in H. miconioides increased with elevation. Glomus was the dominant genus in both root and rhizosphere samples. Elevation significantly influenced the AMF community structure and diversity in the root, with alpha diversity decreasing linearly with elevation. In contrast, no significant elevation-related changes were observed in the rhizosphere soil alpha diversity. The difference in AMF beta diversity between the root and rhizosphere soil was lowest at the highest elevation. Compared to the rhizosphere soil, the degree and degree centralization of AMF community co-occurrence networks in the root showed a significant increase at higher elevations. Variations in soil properties, particularly soil pH, available phosphorus, and total nitrogen levels strongly influenced AMF communities in rhizosphere soil, while nitrate nitrogen, available potassium, and acid phosphatase were correlated with AMF communities in the root. These findings highlight the impact of elevation on AMF communities in both root and rhizosphere soil, providing valuable insights for the habitat restoration and conservation efforts for this species.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of Protoplast Preparation and Establishment of PEG-Mediated Genetic Transformation Method in Cordyceps cicadae.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-03-13 DOI: 10.3390/jof11030219

Haikun Qi, Haihua Ruan, Tao Wu, Hongyang Zhang, Rui Dong, Yanjun Jiang

{"title":"Optimization of Protoplast Preparation and Establishment of PEG-Mediated Genetic Transformation Method in Cordyceps cicadae.","authors":"Haikun Qi, Haihua Ruan, Tao Wu, Hongyang Zhang, Rui Dong, Yanjun Jiang","doi":"10.3390/jof11030219","DOIUrl":"10.3390/jof11030219","url":null,"abstract":"Cordyceps cicadae (C. cicadae) is an important edible medicinal fungus; however, owing to its wild growth and lack of genome annotation, construction of a stable genetic transformation system in C. cicadae is greatly limited, impeding the extensive exploitation of C. cicadae in industry. Here, we successfully established an efficient plasmid transformation method within protoplasts of C. cicadae by PEG mediation using pCas9-EGFP as a marker plasmid. In order to overcome low transformation efficiency and acquire sufficient protoplasts for transformation, the influence of enzyme species, enzymatic hydrolysis time, enzymatic hydrolysis temperature, and fungal age on protoplast preparation were analyzed sequentially, and the optimal conditions for protoplast preparation were determined as follows: 2-day-old C. cicadae mycelia with 1.5% lywallzyme hydrolysis at 34 °C for 5 h. Our results indicate that no less than 5.1 × 107 CFU/mL protoplasts could be acquired. Additionally, five osmotic pressure stabilizers including potassium chloride (KCl), sodium chloride (NaCl), glucose, mannitol, and sucrose were employed to enhance the regeneration of protoplasts, among which sucrose exhibited the highest regeneration rate of 10.43%. The transformation efficiency of plasmid was 37.3 CFU/µg DNA. On this basis, a genetic transformation method was successfully constructed, laying the foundation for further gene editing and metabolic engineering of C. cicadae.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0