Yifan Xue, Yao Wang, Jiang Shi, Jingyao Wei, Qiong Wang, Wenchen Song
{"title":"Plant Functional Traits and Soil Nutrients Drive Divergent Symbiotic Fungal Strategies in Three Urban Street Tree Species.","authors":"Yifan Xue, Yao Wang, Jiang Shi, Jingyao Wei, Qiong Wang, Wenchen Song","doi":"10.3390/jof11060454","DOIUrl":"10.3390/jof11060454","url":null,"abstract":"<p><p>Understanding species-specific mechanisms governing symbiotic fungal responses to plant traits and soil factors is critical for optimizing urban tree \"plant-soil-fungus\" systems under pollution stress. To address this gap, we combined δ<sup>13</sup>C/δ<sup>15</sup>N isotope analysis and ITS sequencing for three common street trees in Beijing: <i>Sophora japonica</i>, <i>Ginkgo biloba</i>, and <i>Populus tomentosa</i>. In <i>S. japonica</i>, symbiotic fungal abundance was positively associated with leaf δ<sup>15</sup>N, indicating root exudate-mediated \"plant-microbe\" interactions during atmospheric NO<sub>x</sub> assimilation. <i>G. biloba</i>, with weak NO<sub>x</sub> assimilation, exhibited a negative correlation between fungal abundance and soil available N/P, suggesting mycorrhizal nutrient compensation under low fertility. <i>P. tomentosa</i> showed decreased fungal abundance with increasing soil N/P ratios and specific leaf area, reflecting carbon allocation trade-offs that limit mycorrhizal investment. These results demonstrate that symbiotic fungi respond to atmospheric and edaphic drivers in a tree species-dependent manner. Urban greening strategies should prioritize <i>S. japonica</i> for its NO<sub>x</sub> mitigation potential and optimize fertilization for <i>G. biloba</i> (nutrient-sensitive fungi) and <i>P. tomentosa</i> (nutrient balance sensitivity). Strategic mixed planting of <i>P. tomentosa</i> with <i>S. japonica</i> could synergistically enhance ecosystem services through complementary resource acquisition patterns. This study provides mechanism-based strategies for optimizing urban tree management under atmospheric pollution stress.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bruno B A Arantes, Ana Karla L F Cabral, Kelvin S Dos Santos, Matheus B Mendonça, Rafaela C Dos Santos, Beatriz C M Bugalho, Lígia De S Fernandes, Luis R Martinez, Ana Marisa Fusco-Almeida, Maria José S Mendes-Giannini
{"title":"Characterization of Biofilm Formation by the Dermatophyte <i>Nannizzia gypsea</i>.","authors":"Bruno B A Arantes, Ana Karla L F Cabral, Kelvin S Dos Santos, Matheus B Mendonça, Rafaela C Dos Santos, Beatriz C M Bugalho, Lígia De S Fernandes, Luis R Martinez, Ana Marisa Fusco-Almeida, Maria José S Mendes-Giannini","doi":"10.3390/jof11060455","DOIUrl":"10.3390/jof11060455","url":null,"abstract":"<p><p>Dermatophytosis is a fungal infection that affects the skin, hair, and nails, impacting approximately 25% of the global population. <i>Nannizzia gypsea</i> is a geophilic fungus that can cause infections in humans and animals. Several studies have been conducted regarding its virulence, or ability to cause disease. This species may produce keratinolytic enzymes and form biofilms, which can increase resistance to treatment. Thus, this study focuses on investigating the biofilm formation of <i>N. gypsea</i> isolated from canine dermatophytosis using an ex vivo hair model, its biofilm extracellular matrix macromolecular contents, and the expression of genes involved in the colonization of keratinized surfaces. The biofilm was analyzed for metabolic activity using the XTT reduction assay, crystal violet staining to measure biofilm biomass, scanning electron microscopy (SEM), and the presence of polysaccharides, proteins, and extracellular DNA in the biofilm extracellular matrix. The virulence genes subtilisin 7, fungalysin (extracellular metalloproteinase), and efflux pump (Multidrug and Toxin Extrusion Protein 2) were evaluated by qPCR, comparing the planktonic and biofilm phenotypes. <i>N. gypsea</i> formed a robust biofilm, which matured after 5 days. Scanning electron microscopy (SEM) revealed the presence of an extensive extracellular matrix. In the hair model, the characteristic ectothrix parasitism of the species is observable. The gene expression analysis revealed a higher expression of all evaluated genes in the biofilm form compared to the planktonic form. Thus, <i>N. gypsea</i> exhibits a biofilm characterized by a robust extracellular matrix and high gene expression of factors related to pathogenesis and resistance.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"<i>Streptomyces</i>-Based Bioformulation to Control Wilt of <i>Morchella sextelata</i> Caused by <i>Pestalotiopsis trachicarpicola</i>.","authors":"Binghan Li, Yue Liu, Aihua Mao, Zhong Hu, Jin Li","doi":"10.3390/jof11060452","DOIUrl":"10.3390/jof11060452","url":null,"abstract":"<p><p>In recent years, there has been extensive documentation of pathogenic fungi infecting <i>Morchella sextelata</i>. However, investigations of microorganisms with antagonistic properties against these pathogens are limited. This study successfully isolated two isolates of the genus <i>Streptomyces</i> (F16 and F19) from the rhizosphere soil of <i>M. sextelata</i> fruiting bodies, both of which exhibit potent antagonistic activity against <i>Pestalotiopsis trachicarpicola</i>, the causative agent of <i>M. sextelata</i> wilt disease. Comprehensive characterization, including physiological-biochemical tests and 16S rDNA sequence analysis, led to the identification of these isolates as <i>Streptomyces</i> sp. F16 and <i>Streptomyces</i> sp. F19. Both isolates significantly inhibited <i>P. trachicarpicola</i> through multiple mechanisms. The volatile compounds produced by these isolates effectively suppressed the conidial germination of <i>P. trachicarpicola</i> in vitro. Furthermore, fermentation filtrates at various dilutions exhibited pronounced antifungal activity against conidial germination, with <i>Streptomyces</i> sp. F16 showing 66.93% inhibition at a 50× dilution and <i>Streptomyces</i> sp. F19 achieving 49.22% inhibition under identical conditions. Field experiments have demonstrated the practical applicability of these antagonists. The topical application of fermentation filtrates (diluted 50×) from both isolates significantly reduced the incidence and severity of disease in <i>M. sextelata</i> cultivation. Notably, the yield improvements were substantial: fields treated with <i>Streptomyces</i> sp. F16 produced 299.6 g/m<sup>2</sup>, whereas those treated with <i>Streptomyces</i> sp. F19 yielded 277.65 g/m<sup>2</sup>. These yields significantly surpassed those of both the untreated control group (231 g/m<sup>2</sup>) and the <i>P. trachicarpicola</i>-inoculated group (134.93 g/m<sup>2</sup>). These findings indicate that the two isolates not only effectively control <i>P. trachicarpicola</i> but also increase the yield of <i>M. sextelata</i>.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marco Pepe, Mohsen Hesami, Livia Fleishmann, Tom Hsiang, Andrew Maxwell Phineas Jones
{"title":"Mycelium Growth and Development of <i>Psilocybe</i> spp. Mother Cultures on Agar-Based Media.","authors":"Marco Pepe, Mohsen Hesami, Livia Fleishmann, Tom Hsiang, Andrew Maxwell Phineas Jones","doi":"10.3390/jof11060450","DOIUrl":"10.3390/jof11060450","url":null,"abstract":"<p><p>The resurgence of interest in the therapeutic potential of psilocybin-producing mushrooms has recently led to numerous research and commercialization efforts. Due to its ease of cultivation and high potency, <i>Psilocybe</i> is the primary genus of interest, and there is a growing need to standardize maintenance, proliferation, and cultivation techniques for efficient and consistent production. The investigation of mycelial growth and development on agar-based media is of principal importance to regulate and optimize mycelium growth and preservation protocols for subsequent fruiting body development. The current investigation is the first to examine the mycelial growth and morphology of four <i>Psilocybe</i> genotypes cultured on different agar-based media. The results from this simple set of experiments provides the foundation for future optimization studies. Ultimately, the information presented can be used to develop genotype-specific mycelial growth and development practices that will shape the future of psychedelic mushroom production for clinical and industrial applications.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genomic and Functional Analysis of a Novel Yeast <i>Cyberlindnera fabianii</i> TBRC 4498 for High-Yield Xylitol Production.","authors":"Pawarin Bonthong, Benjarat Bunterngsook, Wuttichai Mhuantong, Katesuda Aiewviriyasakul, Wipawee Sritusnee, Verawat Champreda, Hataikarn Lekakarn","doi":"10.3390/jof11060453","DOIUrl":"10.3390/jof11060453","url":null,"abstract":"<p><p>The development of yeast cell factories for efficient xylose utilization and xylitol production is crucial for advancing sustainable biotechnological processes. Xylose, a major component of lignocellulosic biomass, presents challenges for microbial conversion due to its complex metabolic pathways. This study presents the genomic perspective and xylitol production capability of a novel xylose utilizing yeast <i>Cyberlindnera fabianii</i> TBRC 4498. Genome sequencing and functional annotation revealed key metabolic networks and genes involved in the xylose metabolism pathway, providing insights into the strain's performance. The <i>Cy. fabianii</i> TBRC 4498 had excellent growth and xylose assimilation at a broad range of xylose concentrations from 40 to 140 g/L, with the highest growth rate at 80 g/L of xylose. The highest xylitol production yield (83.19 g/L) was detected from 120 g/L of xylose at 30 °C for 72 h, equivalent to 0.65 g xylitol/g xylose and 1.16 g/L/h productivity. Remarkably, <i>Cy. fabianii</i> TBRC 4498 produced high-purity xylitol, achieving over 95% homogeneity without forming undesirable byproducts, such as acid or ethanol. These results demonstrated the potential of <i>Cy. fabianii</i> TBRC 4498 as a whole-cell biocatalyst for xylitol production using high xylose concentrations, offering a promising microbial cell factory for large-scale xylitol production from lignocellulosic sugar.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of Homeodomain Proteins at the Aβ Sublocus in <i>Schizophyllum commune</i> and Their Role in Sexual Compatibility and Development.","authors":"Chen Chu, Dongxu Li, Changhong Liu","doi":"10.3390/jof11060451","DOIUrl":"10.3390/jof11060451","url":null,"abstract":"<p><p>The A mating-type locus in <i>Schizophyllum commune</i>, which encodes homeodomain (HD) transcription factors, is essential for regulating sexual compatibility and development. While the role of the Aα sublocus and its Y-Z HD protein complex is adequality understood, the function of HD proteins at the Aβ sublocus remains unclear. In this study, we analyzed the Aβ sublocus of eight monokaryotic <i>S. commune</i> strains derived from the parental dikaryotic strain 20R-7-Z01 and identified four HD genes, <i>abr</i>, <i>abs</i>, <i>abv</i>, and <i>abq</i>, located at the Aβ sublocus. These genes encode two HD1 proteins (S and Q) and two HD2 proteins (R and V). Protein structure prediction, interaction assays, and in vivo functional analyses revealed that R-S and V-Q interactions independently regulate sexual compatibility and fruiting body development. This research highlights the critical role of the Aβ sublocus in fungal reproduction and provides valuable insights for breeding edible and medicinal <i>S. commune</i> strains.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jie Huang, Xingzong Zhang, Wenjing Tan, Yi Wu, Hai Xu, Shuwaner Wang, Sajid Mehmood, Xinli Zhou, Suizhuang Yang, Meinan Wang, Xianming Chen, Wanquan Chen, Taiguo Liu, Xin Li, Chongjing Xia
{"title":"Virulence Characterization of <i>Puccinia striiformis</i> f. sp. <i>tritici</i> in China in 2020 Using Wheat <i>Yr</i> Single-Gene Lines.","authors":"Jie Huang, Xingzong Zhang, Wenjing Tan, Yi Wu, Hai Xu, Shuwaner Wang, Sajid Mehmood, Xinli Zhou, Suizhuang Yang, Meinan Wang, Xianming Chen, Wanquan Chen, Taiguo Liu, Xin Li, Chongjing Xia","doi":"10.3390/jof11060447","DOIUrl":"10.3390/jof11060447","url":null,"abstract":"<p><p>Wheat stripe (yellow) rust, caused by the fungus <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (<i>Pst</i>), is one of the most threatening wheat diseases worldwide. Monitoring the virulence of <i>Pst</i> population is essential for managing wheat stripe rust. In this study, 18 wheat <i>Yr</i> single-gene lines were used to identify the virulence patterns of 67 isolates collected from 13 provinces in China in 2020, from which 33 <i>Pst</i> races were identified. The frequency of virulence to different <i>Yr</i> genes varied from 1.49% to 97.01%, with 4.48% to <i>Yr1</i>, 26.87% to <i>Yr6</i>, 11.94% to <i>Yr7</i>, 95.52% to <i>Yr8</i>, 19.40% to <i>Yr9</i>, 11.94% to <i>Yr17</i>, 2.99% to <i>Yr24</i>, 35.82% to <i>Yr27</i>, 38.81% to <i>Yr43</i>, 97.01% to <i>Yr44</i>, 8.96% to <i>YrSP</i>, 1.49% to <i>Yr85</i>, 95.52% to <i>YrExp2</i>, and 7.46% to <i>Yr76</i>. None of the isolates were virulent to <i>Yr5</i>, <i>Yr10</i>, <i>Yr15</i>, and <i>Yr32</i>. Among the 33 races, PstCN-062 (with virulence to <i>Yr8</i>, <i>Yr44</i>, and <i>YrExp2</i>) and PstCN-001 (with virulence to <i>Yr8</i>, <i>Yr43</i>, <i>Yr44</i>, and <i>YrExp2</i>) were the prevalent races, with frequencies of 28.36% and 11.94%, respectively. These results provide valuable information for breeding resistant wheat cultivars for controlling stripe rust.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Engineering of Global Transcriptional Regulators (GTRs) in <i>Aspergillus</i> for Natural Product Discovery.","authors":"Yujie Zhao, Qing Gong, Huawei Zhang","doi":"10.3390/jof11060449","DOIUrl":"10.3390/jof11060449","url":null,"abstract":"<p><p>The <i>Aspergillus</i> genus is an important group of filamentous fungi, and the various biological activities of its secondary metabolites (SMs) have great biosynthetic potential. Despite over 4200 SMs having been isolated from <i>Aspergillus</i> spp., their metabolic potential remains unexplored due to the presence of numerous silent biosynthetic gene clusters (BGCs) in their genomes. Fortunately, over the last two decades, the global transcriptional regulator (GTR) engineering strategy has emerged as a powerful tool for activating these cryptic BGCs in order to synthesize previously undiscovered SMs from <i>Aspergillus</i> spp. This review highlights recent advances in fungal GTR engineering techniques, the regulatory mechanisms of GTRs, and current challenges and future perspectives for their application in natural product discovery in the genus <i>Aspergillus</i>.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L Bernal-Martínez, P De la Cruz-Ríos, R Viedma, S Gago, S Ortega-Madueño, L Alcazar-Fuoli, M J Buitrago
{"title":"Usefulness of Serum as a Non-Invasive Sample for the Detection of <i>Histoplasma capsulatum</i> Infections: Retrospective Comparative Analysis of Different Diagnostic Techniques and Quantification of Host Biomarkers.","authors":"L Bernal-Martínez, P De la Cruz-Ríos, R Viedma, S Gago, S Ortega-Madueño, L Alcazar-Fuoli, M J Buitrago","doi":"10.3390/jof11060448","DOIUrl":"10.3390/jof11060448","url":null,"abstract":"<p><p>Diagnosis of histoplasmosis is challenging. A rapid, sensitive, and specific method is essential. Serum is a non-invasive and easy sample to obtain in any hospital. The diagnostic accuracy of different techniques that use serum has been evaluated. Forty-one serum samples from patients with proven or probable histoplasmosis were analyzed. Different diagnostic techniques based on the detection of antibodies (ID Fungal Antibody System), antigens (Histoplasma GM EIA and Platelia<sup>TM</sup> Aspergillus Ag), and DNA (\"in-house\" real-time PCR (RT-PCR) were tested and compared. Additionally, the quantification of cytokines and biomarkers related to histoplasmosis was performed. Global results from 27 samples in which all the tests were performed showed that the sensitivity of the Histoplasma GM EIA kit was 87.5% in patients with disseminated infection and HIV as an underlying disease; in immunocompetent (IC) patients, it was 54.5%. The detection of <i>Histoplasma</i> spp. with the ID Fungal Antibody System was positive in 90.9% of IC and in 62.5% of HIV patients. The Platelia-Asp kit had a low performance in both groups of patients (37.5% in HIV and 9% in non-HIV), and, finally, RT-PCR was better in immunosuppressed patients (44% in HIV vs. 27% in non-HIV). The combination of diagnostic techniques increased the detection of Histoplasma infection in inmunosupressed patients. Overall, patient groups infected with H. capsulatum (Hc) showed higher IL-8, IL-6, IL-1β, TNF-α, and IL-18 median values compared to non-Hc-infected controls. The effectiveness of diagnostic techniques on serum samples is highly influenced by the patient's clinical presentation and underlying condition. Consequently, a thorough assessment of the patient's clinical presentation and disease phenotype is crucial in selecting the most suitable diagnostic method.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paul J Resong, Joseph Lee, Adam Vazquez, David Hess, Kirk Bronander, Samuel A Lee
{"title":"Genetic Confirmation of Clonal Spread of <i>Candida auris</i> from Southern to Northern Nevada.","authors":"Paul J Resong, Joseph Lee, Adam Vazquez, David Hess, Kirk Bronander, Samuel A Lee","doi":"10.3390/jof11060445","DOIUrl":"10.3390/jof11060445","url":null,"abstract":"<p><p><i>Candida auris</i> is an emerging fungal pathogen characterized by high levels of antifungal drug resistance and hospital outbreaks in a global distribution. Since introduction to the United States, it has been identified most frequently in New York, Illinois, California, Florida, and Nevada. Its surge poses significant risk as a nosocomial infection with multi-drug resistance, with clades bearing resistance to fluconazole, micafungin, and amphotericin B. Within the state of Nevada, and specifically the greater Las Vegas area in the southern part of the state, there are ongoing outbreaks from clade I and clade III, with 1728 confirmed clinical cases identified as of January 2025. In northern Nevada, three clinical cases have been identified to date, with two occurring at our facility. Both patients had been hospitalized at facilities in Las Vegas, Nevada. The <i>C. auris</i> strains isolated from these two cases have been identified as belonging to clade III and demonstrate resistance to fluconazole. Genome sequencing of the <i>C. auris</i> isolates indicates close genetic identity to strains from the Las Vegas outbreak. These data indicate that the spread of these clonal isolates is due to hospitalization and subsequent patient relocation to northern Nevada, revealing the ongoing importance of screening for geographic spread.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}