Journal of Fungi最新文献

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Plant Functional Traits and Soil Nutrients Drive Divergent Symbiotic Fungal Strategies in Three Urban Street Tree Species. 植物功能性状和土壤养分驱动三种城市行道树不同的共生真菌策略
IF 4.2 2区 生物学
Journal of Fungi Pub Date : 2025-06-14 DOI: 10.3390/jof11060454
Yifan Xue, Yao Wang, Jiang Shi, Jingyao Wei, Qiong Wang, Wenchen Song
{"title":"Plant Functional Traits and Soil Nutrients Drive Divergent Symbiotic Fungal Strategies in Three Urban Street Tree Species.","authors":"Yifan Xue, Yao Wang, Jiang Shi, Jingyao Wei, Qiong Wang, Wenchen Song","doi":"10.3390/jof11060454","DOIUrl":"10.3390/jof11060454","url":null,"abstract":"<p><p>Understanding species-specific mechanisms governing symbiotic fungal responses to plant traits and soil factors is critical for optimizing urban tree \"plant-soil-fungus\" systems under pollution stress. To address this gap, we combined δ<sup>13</sup>C/δ<sup>15</sup>N isotope analysis and ITS sequencing for three common street trees in Beijing: <i>Sophora japonica</i>, <i>Ginkgo biloba</i>, and <i>Populus tomentosa</i>. In <i>S. japonica</i>, symbiotic fungal abundance was positively associated with leaf δ<sup>15</sup>N, indicating root exudate-mediated \"plant-microbe\" interactions during atmospheric NO<sub>x</sub> assimilation. <i>G. biloba</i>, with weak NO<sub>x</sub> assimilation, exhibited a negative correlation between fungal abundance and soil available N/P, suggesting mycorrhizal nutrient compensation under low fertility. <i>P. tomentosa</i> showed decreased fungal abundance with increasing soil N/P ratios and specific leaf area, reflecting carbon allocation trade-offs that limit mycorrhizal investment. These results demonstrate that symbiotic fungi respond to atmospheric and edaphic drivers in a tree species-dependent manner. Urban greening strategies should prioritize <i>S. japonica</i> for its NO<sub>x</sub> mitigation potential and optimize fertilization for <i>G. biloba</i> (nutrient-sensitive fungi) and <i>P. tomentosa</i> (nutrient balance sensitivity). Strategic mixed planting of <i>P. tomentosa</i> with <i>S. japonica</i> could synergistically enhance ecosystem services through complementary resource acquisition patterns. This study provides mechanism-based strategies for optimizing urban tree management under atmospheric pollution stress.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of Biofilm Formation by the Dermatophyte Nannizzia gypsea. 南褐皮菌形成生物膜的特性研究。
IF 4.2 2区 生物学
Journal of Fungi Pub Date : 2025-06-14 DOI: 10.3390/jof11060455
Bruno B A Arantes, Ana Karla L F Cabral, Kelvin S Dos Santos, Matheus B Mendonça, Rafaela C Dos Santos, Beatriz C M Bugalho, Lígia De S Fernandes, Luis R Martinez, Ana Marisa Fusco-Almeida, Maria José S Mendes-Giannini
{"title":"Characterization of Biofilm Formation by the Dermatophyte <i>Nannizzia gypsea</i>.","authors":"Bruno B A Arantes, Ana Karla L F Cabral, Kelvin S Dos Santos, Matheus B Mendonça, Rafaela C Dos Santos, Beatriz C M Bugalho, Lígia De S Fernandes, Luis R Martinez, Ana Marisa Fusco-Almeida, Maria José S Mendes-Giannini","doi":"10.3390/jof11060455","DOIUrl":"10.3390/jof11060455","url":null,"abstract":"<p><p>Dermatophytosis is a fungal infection that affects the skin, hair, and nails, impacting approximately 25% of the global population. <i>Nannizzia gypsea</i> is a geophilic fungus that can cause infections in humans and animals. Several studies have been conducted regarding its virulence, or ability to cause disease. This species may produce keratinolytic enzymes and form biofilms, which can increase resistance to treatment. Thus, this study focuses on investigating the biofilm formation of <i>N. gypsea</i> isolated from canine dermatophytosis using an ex vivo hair model, its biofilm extracellular matrix macromolecular contents, and the expression of genes involved in the colonization of keratinized surfaces. The biofilm was analyzed for metabolic activity using the XTT reduction assay, crystal violet staining to measure biofilm biomass, scanning electron microscopy (SEM), and the presence of polysaccharides, proteins, and extracellular DNA in the biofilm extracellular matrix. The virulence genes subtilisin 7, fungalysin (extracellular metalloproteinase), and efflux pump (Multidrug and Toxin Extrusion Protein 2) were evaluated by qPCR, comparing the planktonic and biofilm phenotypes. <i>N. gypsea</i> formed a robust biofilm, which matured after 5 days. Scanning electron microscopy (SEM) revealed the presence of an extensive extracellular matrix. In the hair model, the characteristic ectothrix parasitism of the species is observable. The gene expression analysis revealed a higher expression of all evaluated genes in the biofilm form compared to the planktonic form. Thus, <i>N. gypsea</i> exhibits a biofilm characterized by a robust extracellular matrix and high gene expression of factors related to pathogenesis and resistance.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Streptomyces-Based Bioformulation to Control Wilt of Morchella sextelata Caused by Pestalotiopsis trachicarpicola. 链霉菌生物制剂防治细盘拟盘多毛孢引起的六口羊肚菌枯萎病。
IF 4.2 2区 生物学
Journal of Fungi Pub Date : 2025-06-13 DOI: 10.3390/jof11060452
Binghan Li, Yue Liu, Aihua Mao, Zhong Hu, Jin Li
{"title":"<i>Streptomyces</i>-Based Bioformulation to Control Wilt of <i>Morchella sextelata</i> Caused by <i>Pestalotiopsis trachicarpicola</i>.","authors":"Binghan Li, Yue Liu, Aihua Mao, Zhong Hu, Jin Li","doi":"10.3390/jof11060452","DOIUrl":"10.3390/jof11060452","url":null,"abstract":"<p><p>In recent years, there has been extensive documentation of pathogenic fungi infecting <i>Morchella sextelata</i>. However, investigations of microorganisms with antagonistic properties against these pathogens are limited. This study successfully isolated two isolates of the genus <i>Streptomyces</i> (F16 and F19) from the rhizosphere soil of <i>M. sextelata</i> fruiting bodies, both of which exhibit potent antagonistic activity against <i>Pestalotiopsis trachicarpicola</i>, the causative agent of <i>M. sextelata</i> wilt disease. Comprehensive characterization, including physiological-biochemical tests and 16S rDNA sequence analysis, led to the identification of these isolates as <i>Streptomyces</i> sp. F16 and <i>Streptomyces</i> sp. F19. Both isolates significantly inhibited <i>P. trachicarpicola</i> through multiple mechanisms. The volatile compounds produced by these isolates effectively suppressed the conidial germination of <i>P. trachicarpicola</i> in vitro. Furthermore, fermentation filtrates at various dilutions exhibited pronounced antifungal activity against conidial germination, with <i>Streptomyces</i> sp. F16 showing 66.93% inhibition at a 50× dilution and <i>Streptomyces</i> sp. F19 achieving 49.22% inhibition under identical conditions. Field experiments have demonstrated the practical applicability of these antagonists. The topical application of fermentation filtrates (diluted 50×) from both isolates significantly reduced the incidence and severity of disease in <i>M. sextelata</i> cultivation. Notably, the yield improvements were substantial: fields treated with <i>Streptomyces</i> sp. F16 produced 299.6 g/m<sup>2</sup>, whereas those treated with <i>Streptomyces</i> sp. F19 yielded 277.65 g/m<sup>2</sup>. These yields significantly surpassed those of both the untreated control group (231 g/m<sup>2</sup>) and the <i>P. trachicarpicola</i>-inoculated group (134.93 g/m<sup>2</sup>). These findings indicate that the two isolates not only effectively control <i>P. trachicarpicola</i> but also increase the yield of <i>M. sextelata</i>.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mycelium Growth and Development of Psilocybe spp. Mother Cultures on Agar-Based Media. 裸盖菇菌丝在琼脂培养基上的生长发育。
IF 4.2 2区 生物学
Journal of Fungi Pub Date : 2025-06-13 DOI: 10.3390/jof11060450
Marco Pepe, Mohsen Hesami, Livia Fleishmann, Tom Hsiang, Andrew Maxwell Phineas Jones
{"title":"Mycelium Growth and Development of <i>Psilocybe</i> spp. Mother Cultures on Agar-Based Media.","authors":"Marco Pepe, Mohsen Hesami, Livia Fleishmann, Tom Hsiang, Andrew Maxwell Phineas Jones","doi":"10.3390/jof11060450","DOIUrl":"10.3390/jof11060450","url":null,"abstract":"<p><p>The resurgence of interest in the therapeutic potential of psilocybin-producing mushrooms has recently led to numerous research and commercialization efforts. Due to its ease of cultivation and high potency, <i>Psilocybe</i> is the primary genus of interest, and there is a growing need to standardize maintenance, proliferation, and cultivation techniques for efficient and consistent production. The investigation of mycelial growth and development on agar-based media is of principal importance to regulate and optimize mycelium growth and preservation protocols for subsequent fruiting body development. The current investigation is the first to examine the mycelial growth and morphology of four <i>Psilocybe</i> genotypes cultured on different agar-based media. The results from this simple set of experiments provides the foundation for future optimization studies. Ultimately, the information presented can be used to develop genotype-specific mycelial growth and development practices that will shape the future of psychedelic mushroom production for clinical and industrial applications.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194638/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genomic and Functional Analysis of a Novel Yeast Cyberlindnera fabianii TBRC 4498 for High-Yield Xylitol Production. 高产木糖醇新酵母菌fabianii TBRC 4498的基因组学和功能分析
IF 4.2 2区 生物学
Journal of Fungi Pub Date : 2025-06-13 DOI: 10.3390/jof11060453
Pawarin Bonthong, Benjarat Bunterngsook, Wuttichai Mhuantong, Katesuda Aiewviriyasakul, Wipawee Sritusnee, Verawat Champreda, Hataikarn Lekakarn
{"title":"Genomic and Functional Analysis of a Novel Yeast <i>Cyberlindnera fabianii</i> TBRC 4498 for High-Yield Xylitol Production.","authors":"Pawarin Bonthong, Benjarat Bunterngsook, Wuttichai Mhuantong, Katesuda Aiewviriyasakul, Wipawee Sritusnee, Verawat Champreda, Hataikarn Lekakarn","doi":"10.3390/jof11060453","DOIUrl":"10.3390/jof11060453","url":null,"abstract":"<p><p>The development of yeast cell factories for efficient xylose utilization and xylitol production is crucial for advancing sustainable biotechnological processes. Xylose, a major component of lignocellulosic biomass, presents challenges for microbial conversion due to its complex metabolic pathways. This study presents the genomic perspective and xylitol production capability of a novel xylose utilizing yeast <i>Cyberlindnera fabianii</i> TBRC 4498. Genome sequencing and functional annotation revealed key metabolic networks and genes involved in the xylose metabolism pathway, providing insights into the strain's performance. The <i>Cy. fabianii</i> TBRC 4498 had excellent growth and xylose assimilation at a broad range of xylose concentrations from 40 to 140 g/L, with the highest growth rate at 80 g/L of xylose. The highest xylitol production yield (83.19 g/L) was detected from 120 g/L of xylose at 30 °C for 72 h, equivalent to 0.65 g xylitol/g xylose and 1.16 g/L/h productivity. Remarkably, <i>Cy. fabianii</i> TBRC 4498 produced high-purity xylitol, achieving over 95% homogeneity without forming undesirable byproducts, such as acid or ethanol. These results demonstrated the potential of <i>Cy. fabianii</i> TBRC 4498 as a whole-cell biocatalyst for xylitol production using high xylose concentrations, offering a promising microbial cell factory for large-scale xylitol production from lignocellulosic sugar.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of Homeodomain Proteins at the Aβ Sublocus in Schizophyllum commune and Their Role in Sexual Compatibility and Development. 裂叶植物Aβ亚座同源结构域蛋白的特征及其在性相容性和发育中的作用。
IF 4.2 2区 生物学
Journal of Fungi Pub Date : 2025-06-13 DOI: 10.3390/jof11060451
Chen Chu, Dongxu Li, Changhong Liu
{"title":"Characterization of Homeodomain Proteins at the Aβ Sublocus in <i>Schizophyllum commune</i> and Their Role in Sexual Compatibility and Development.","authors":"Chen Chu, Dongxu Li, Changhong Liu","doi":"10.3390/jof11060451","DOIUrl":"10.3390/jof11060451","url":null,"abstract":"<p><p>The A mating-type locus in <i>Schizophyllum commune</i>, which encodes homeodomain (HD) transcription factors, is essential for regulating sexual compatibility and development. While the role of the Aα sublocus and its Y-Z HD protein complex is adequality understood, the function of HD proteins at the Aβ sublocus remains unclear. In this study, we analyzed the Aβ sublocus of eight monokaryotic <i>S. commune</i> strains derived from the parental dikaryotic strain 20R-7-Z01 and identified four HD genes, <i>abr</i>, <i>abs</i>, <i>abv</i>, and <i>abq</i>, located at the Aβ sublocus. These genes encode two HD1 proteins (S and Q) and two HD2 proteins (R and V). Protein structure prediction, interaction assays, and in vivo functional analyses revealed that R-S and V-Q interactions independently regulate sexual compatibility and fruiting body development. This research highlights the critical role of the Aβ sublocus in fungal reproduction and provides valuable insights for breeding edible and medicinal <i>S. commune</i> strains.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Virulence Characterization of Puccinia striiformis f. sp. tritici in China in 2020 Using Wheat Yr Single-Gene Lines. 利用小麦单基因品系对2020年中国小麦纹状锈菌毒力的研究
IF 4.2 2区 生物学
Journal of Fungi Pub Date : 2025-06-12 DOI: 10.3390/jof11060447
Jie Huang, Xingzong Zhang, Wenjing Tan, Yi Wu, Hai Xu, Shuwaner Wang, Sajid Mehmood, Xinli Zhou, Suizhuang Yang, Meinan Wang, Xianming Chen, Wanquan Chen, Taiguo Liu, Xin Li, Chongjing Xia
{"title":"Virulence Characterization of <i>Puccinia striiformis</i> f. sp. <i>tritici</i> in China in 2020 Using Wheat <i>Yr</i> Single-Gene Lines.","authors":"Jie Huang, Xingzong Zhang, Wenjing Tan, Yi Wu, Hai Xu, Shuwaner Wang, Sajid Mehmood, Xinli Zhou, Suizhuang Yang, Meinan Wang, Xianming Chen, Wanquan Chen, Taiguo Liu, Xin Li, Chongjing Xia","doi":"10.3390/jof11060447","DOIUrl":"10.3390/jof11060447","url":null,"abstract":"<p><p>Wheat stripe (yellow) rust, caused by the fungus <i>Puccinia striiformis</i> f. sp. <i>tritici</i> (<i>Pst</i>), is one of the most threatening wheat diseases worldwide. Monitoring the virulence of <i>Pst</i> population is essential for managing wheat stripe rust. In this study, 18 wheat <i>Yr</i> single-gene lines were used to identify the virulence patterns of 67 isolates collected from 13 provinces in China in 2020, from which 33 <i>Pst</i> races were identified. The frequency of virulence to different <i>Yr</i> genes varied from 1.49% to 97.01%, with 4.48% to <i>Yr1</i>, 26.87% to <i>Yr6</i>, 11.94% to <i>Yr7</i>, 95.52% to <i>Yr8</i>, 19.40% to <i>Yr9</i>, 11.94% to <i>Yr17</i>, 2.99% to <i>Yr24</i>, 35.82% to <i>Yr27</i>, 38.81% to <i>Yr43</i>, 97.01% to <i>Yr44</i>, 8.96% to <i>YrSP</i>, 1.49% to <i>Yr85</i>, 95.52% to <i>YrExp2</i>, and 7.46% to <i>Yr76</i>. None of the isolates were virulent to <i>Yr5</i>, <i>Yr10</i>, <i>Yr15</i>, and <i>Yr32</i>. Among the 33 races, PstCN-062 (with virulence to <i>Yr8</i>, <i>Yr44</i>, and <i>YrExp2</i>) and PstCN-001 (with virulence to <i>Yr8</i>, <i>Yr43</i>, <i>Yr44</i>, and <i>YrExp2</i>) were the prevalent races, with frequencies of 28.36% and 11.94%, respectively. These results provide valuable information for breeding resistant wheat cultivars for controlling stripe rust.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineering of Global Transcriptional Regulators (GTRs) in Aspergillus for Natural Product Discovery. 曲霉中用于天然产物发现的全局转录调控因子(GTRs)工程。
IF 4.2 2区 生物学
Journal of Fungi Pub Date : 2025-06-12 DOI: 10.3390/jof11060449
Yujie Zhao, Qing Gong, Huawei Zhang
{"title":"Engineering of Global Transcriptional Regulators (GTRs) in <i>Aspergillus</i> for Natural Product Discovery.","authors":"Yujie Zhao, Qing Gong, Huawei Zhang","doi":"10.3390/jof11060449","DOIUrl":"10.3390/jof11060449","url":null,"abstract":"<p><p>The <i>Aspergillus</i> genus is an important group of filamentous fungi, and the various biological activities of its secondary metabolites (SMs) have great biosynthetic potential. Despite over 4200 SMs having been isolated from <i>Aspergillus</i> spp., their metabolic potential remains unexplored due to the presence of numerous silent biosynthetic gene clusters (BGCs) in their genomes. Fortunately, over the last two decades, the global transcriptional regulator (GTR) engineering strategy has emerged as a powerful tool for activating these cryptic BGCs in order to synthesize previously undiscovered SMs from <i>Aspergillus</i> spp. This review highlights recent advances in fungal GTR engineering techniques, the regulatory mechanisms of GTRs, and current challenges and future perspectives for their application in natural product discovery in the genus <i>Aspergillus</i>.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Usefulness of Serum as a Non-Invasive Sample for the Detection of Histoplasma capsulatum Infections: Retrospective Comparative Analysis of Different Diagnostic Techniques and Quantification of Host Biomarkers. 血清作为无创样本检测荚膜组织浆体感染的有效性:不同诊断技术和宿主生物标志物定量的回顾性比较分析。
IF 4.2 2区 生物学
Journal of Fungi Pub Date : 2025-06-12 DOI: 10.3390/jof11060448
L Bernal-Martínez, P De la Cruz-Ríos, R Viedma, S Gago, S Ortega-Madueño, L Alcazar-Fuoli, M J Buitrago
{"title":"Usefulness of Serum as a Non-Invasive Sample for the Detection of <i>Histoplasma capsulatum</i> Infections: Retrospective Comparative Analysis of Different Diagnostic Techniques and Quantification of Host Biomarkers.","authors":"L Bernal-Martínez, P De la Cruz-Ríos, R Viedma, S Gago, S Ortega-Madueño, L Alcazar-Fuoli, M J Buitrago","doi":"10.3390/jof11060448","DOIUrl":"10.3390/jof11060448","url":null,"abstract":"<p><p>Diagnosis of histoplasmosis is challenging. A rapid, sensitive, and specific method is essential. Serum is a non-invasive and easy sample to obtain in any hospital. The diagnostic accuracy of different techniques that use serum has been evaluated. Forty-one serum samples from patients with proven or probable histoplasmosis were analyzed. Different diagnostic techniques based on the detection of antibodies (ID Fungal Antibody System), antigens (Histoplasma GM EIA and Platelia<sup>TM</sup> Aspergillus Ag), and DNA (\"in-house\" real-time PCR (RT-PCR) were tested and compared. Additionally, the quantification of cytokines and biomarkers related to histoplasmosis was performed. Global results from 27 samples in which all the tests were performed showed that the sensitivity of the Histoplasma GM EIA kit was 87.5% in patients with disseminated infection and HIV as an underlying disease; in immunocompetent (IC) patients, it was 54.5%. The detection of <i>Histoplasma</i> spp. with the ID Fungal Antibody System was positive in 90.9% of IC and in 62.5% of HIV patients. The Platelia-Asp kit had a low performance in both groups of patients (37.5% in HIV and 9% in non-HIV), and, finally, RT-PCR was better in immunosuppressed patients (44% in HIV vs. 27% in non-HIV). The combination of diagnostic techniques increased the detection of Histoplasma infection in inmunosupressed patients. Overall, patient groups infected with H. capsulatum (Hc) showed higher IL-8, IL-6, IL-1β, TNF-α, and IL-18 median values compared to non-Hc-infected controls. The effectiveness of diagnostic techniques on serum samples is highly influenced by the patient's clinical presentation and underlying condition. Consequently, a thorough assessment of the patient's clinical presentation and disease phenotype is crucial in selecting the most suitable diagnostic method.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic Confirmation of Clonal Spread of Candida auris from Southern to Northern Nevada. 内华达南部到北部耳念珠菌克隆传播的遗传学证实。
IF 4.2 2区 生物学
Journal of Fungi Pub Date : 2025-06-12 DOI: 10.3390/jof11060445
Paul J Resong, Joseph Lee, Adam Vazquez, David Hess, Kirk Bronander, Samuel A Lee
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