Journal of Fungi最新文献

{"title":"Curvulones D-E and Bioactive Cytochalasans from <i>Macrozamia communis</i>-Associated Fungus <i>Penicillium</i> sp. MNP-GP-4.","authors":"Sherif S Ebada, Mohamed S Elnaggar, Ahmed M Elissawy, Rémy Bertrand Teponno, Noureddine Bouras, Wael M Afifi, Nehal Ibrahim, Abdel Nasser B Singab, Nicole Teusch","doi":"10.3390/jof12100786","DOIUrl":"10.3390/jof12100786","url":null,"abstract":"<p><p><i>Macrozamia communis</i> (Australian cycad) is the most abundant cycad in Australia, yet its associated fungi lack chemical investigation. As a continuation of our ongoing studies on <i>M. communis</i>-associated fungi, an in-depth chemical exploration of the fermented fungal biomass of <i>Penicillium</i> sp. MNP-GP-4 afforded the identification of two previously undescribed curvularin derivatives, curvulones D (<b>1</b>) and E (<b>2</b>), together with three known metabolites, including curvularin (<b>3</b>) and two cytochalasan derivatives chaetoglobosins E and Fex (<b>4</b> and <b>5</b>). Chemical structures of the isolated compounds were elucidated based on comprehensive 1D and 2D NMR spectroscopic analyses and HR-(+)ESI-MS and comparison with the reported literature. All the isolated secondary metabolites were evaluated for their cytotoxic activity against a pancreatic duct adenocarcinoma (PDAC) cell line, where compounds <b>4</b> and <b>5</b> were the most active with IC₅₀ values of 2.9 and 3.7 µM, respectively.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"12 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13705659/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149268748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solid-State Fermentation of <i>Ganoderma lucidum</i> on Brewer's Spent Grain and Sawdust for Mycelium-Based Biomaterial Production.","authors":"Nelson Dueñas, Richelly Celedon, Doménica Salomé Araque Buitrón, María Alejandra Cruz","doi":"10.3390/jof12100788","DOIUrl":"10.3390/jof12100788","url":null,"abstract":"<p><p><i>Ganoderma lucidum</i> is a widely studied fungal species due to its potential for producing mycelium-based biomaterials. In mycotecture applications, fungal mycelium colonizes organic substrates, forming composite structures that can be used as sustainable biomaterials. Meanwhile, the management of agro-industrial and wood residues remains an environmental challenge, as these materials are often discarded or burned. This study aimed to evaluate the effects of substrate composition and inoculum load on the recovered dry fraction obtained during the solid-state fermentation (SSF) of <i>G. lucidum</i> using brewer's spent grain (BSG), an agro-industrial by-product of beer production, and sawdust, a wood-processing residue. An exploratory approach based on a reduced central composite design (CCD) was used to evaluate the effects of inoculum load and substrate composition on the recovered dry fraction. The apparent specific growth rate (µ) during the observed apparent exponential growth interval (days 3-6) and the corresponding doubling time (td) were also estimated. The treatment associated with the highest recovered dry fraction contained 40.13% (<i>w</i>/<i>w</i>) sawdust and 59.87% (<i>w</i>/<i>w</i>) BSG, with an inoculum load of 2.69% (<i>v</i>/<i>w</i>). Growth kinetics were subsequently estimated under these conditions. The apparent specific growth rate over days 3-6 was 0.6346 day^-1, corresponding to a doubling time of 1.0922 days. Substrate composition was the main factor associated with variation in the recovered dry fraction. These findings suggest that BSG and sawdust may be suitable substrate components for valorization through the SSF of <i>G. lucidum</i>. However, the recovered dry fraction is an operational measurement and should not be interpreted as a direct quantification of pure fungal biomass. The resulting specimens produced in this study exhibited a density of 0.24-0.27 g cm^-3 and a compressive strength of 550-700 kPa.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"12 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13706297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149268848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subcellular Localization of Nicotinic Acid Utilization Pathway Proteins in <i>Aspergillus nidulans</i> and a Putative HxnX-Dependent Piggyback Import of HxnW.","authors":"Suren Nemuuzaya, Judit Ámon, Gabriella Varga, Eszter Bokor, Kitti Vecsernyés-Nagy, Zsuzsanna Hamari","doi":"10.3390/jof12100785","DOIUrl":"10.3390/jof12100785","url":null,"abstract":"<p><p>A recently discovered eukaryotic nicotinic acid catabolic pathway involves the concerted action of eight Hxn enzymes including HxnX, a peroxisomal enzyme carrying a canonical PTS1 motif (SRL) at its C-terminus. None of the seven remaining pathway enzymes contains an obvious peroxisomal targeting signal (PTS). However, the nicotinic acid transporters HxnP and HxnZ, and the 2,5-dihydroxypyridine hydroxylase HxnV, carry non-canonical PTS1 motifs originally described in plants. N-terminal Gfp fusions localized HxnP and HxnZ to the plasma membrane and septa, whereas Gfp-HxnV showed diffuse cytoplasmic localization, with no detectable peroxisomal targeting. The <i>hxnX</i> and <i>hxnW</i> genes, which encode HxnX and the 5,6-dihydroxypiperidine-2-one ketoreductase HxnW, respectively, are divergently transcribed from a shared intergenic region, suggesting coordinated regulation of their expression and raising the possibility of a functional association between the two proteins. Consistent with this possibility, bimolecular fluorescence complementation (BiFC) provided evidence for an interaction between HxnX and HxnW. However, neither the BiFC fusion proteins nor the Gfp-HxnW fusion complemented the nicotinic acid utilization defects of the <i>hxnXΔ hxnWΔ</i> and <i>hxnWΔ</i> strains, respectively, and neither showed peroxisomal localization. These findings indicate that fusion of Gfp or split-Yfp fragments interferes with HxnW function, potentially by affecting protein folding, catalytic activity, protein-protein interaction, or intracellular targeting. Thus, while the observed HxnX-HxnW interaction is consistent with a possible piggyback-mediated import mechanism, the peroxisomal import of HxnW remains to be demonstrated experimentally.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"12 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13705651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149268983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Course of Four Patients with Mucormycosis Managed Predominantly with Ayurveda-Based Interventions: A Case Series.","authors":"Rajnikant Patel, Skanthesh Lakshmanan, Vinayak Padikkal, Maël Voegeli, Keyur Mandaliya","doi":"10.3390/jof12100781","DOIUrl":"10.3390/jof12100781","url":null,"abstract":"<p><p>Mucormycosis is a rapidly progressive angioinvasive fungal infection caused by Mucorales, predominantly affecting immunocompromised individuals and associated with substantial morbidity and mortality. Despite advances in standard care involving surgical debridement and systemic antifungal therapy, outcomes may remain suboptimal because of recurrence, treatment-related toxicity, including nephrotoxicity associated with amphotericin B, and substantial economic burden. These challenges justify continued investigation of adjunctive therapies and other approaches that may potentially improve outcomes or reduce treatment burden, while established antifungal therapy and surgical management remain the cornerstone of care. Ayurveda, a traditional system of medicine in India, employs comprehensive therapeutic strategies that may include oral formulations, local interventions, dietary regulation, and lifestyle modifications. Ayurveda has been utilised by patients with mucormycosis; however, its evidence base remains limited and largely restricted to adjunctive or isolated case reports. This paper presents a descriptive case series of four patients diagnosed with mucormycosis who were managed predominantly with Ayurveda-based interventions. Three patients opted to receive stand-alone Ayurveda-based treatment after declining recommended standard biomedical treatment. All patients had complex comorbidities, including diabetes mellitus, hypertension, and renal impairment. Serial follow-up imaging documented radiological changes interpreted by the reporting radiologists as improvement in three patients, with no documented recurrence during the available follow-up periods. One patient with suboptimal treatment adherence showed persistent radiological abnormalities at the three-month assessment, although survival was maintained for approximately one year following diagnosis. No treatment-related adverse events were documented in the available follow-up records; one patient with pre-existing renal impairment showed an increase in serum creatinine from 1.48 to 3.3 mg/dL during follow-up. These observations are descriptive and derive from an uncontrolled case series with non-standardised follow-up and outcome assessment. They do not establish treatment effectiveness, safety, non-inferiority, or suitability for clinical application and should not be interpreted as evidence supporting replacement of current standard-of-care management. Rather, these observations advance ongoing hypothesis generation and provide conceptual and methodological insights for the development of future clinical research protocols evaluating predominantly Ayurveda-based interventions for mucormycosis care.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"12 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13706485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149268782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory Effects and Transcriptomic Insights of L-Arginine Against <i>Diaporthe nobilis</i>.","authors":"Shuang Zhou, Xuesong Wang, Ziye Wang, Lifeng Duan, Jianhua Feng, Dong-Hui Yan","doi":"10.3390/jof12100783","DOIUrl":"10.3390/jof12100783","url":null,"abstract":"<p><p><i>Diaporthe nobilis</i> causes postharvest decay in Chinese chestnuts (<i>Castanea mollissima</i> Blume). This study evaluated the effects of exogenous L-arginine (L-Arg) on fungal growth, fruit decay, and transcriptional responses using a single <i>D. nobilis</i> isolate. In a preliminary in vitro screen of nine amino acids at 30 mM, L-Arg showed the strongest inhibition of mycelial growth. L-Arg inhibited mycelial growth in a concentration-dependent manner in vitro. In the single fruit assay, the 2.5-20 mM groups had lower observed disease incidence and disease indices than the water control, whereas the 30 mM group had values close to the control. Transcriptomic analysis of mycelia grown on potato dextrose agar (PDA) showed lower transcript levels for several genes annotated as encoding membrane transporters, cell wall-degrading enzymes (CWDEs), and proteins involved in secondary metabolism under L-Arg treatment. These findings describe transcriptional responses under in vitro culture conditions. Without functional or biochemical validation, they do not establish reduced CWDE activity or suppression of virulence during host infection. The contrasting responses of fungal growth and fruit decay indicate that stronger inhibition in vitro does not necessarily translate into better disease control. Further studies across isolates, together with measurements during infection and functional validation, are needed to assess the mechanisms and practical value of L-Arg for postharvest chestnut protection.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"12 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13706520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149268742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal Activity of <i>Streptomyces</i> sp. F204 Against Rhizoctonia Solani and Whole-Genome Sequencing and Metabolomic Analysis.","authors":"Longhui Shen, Jinyan Xue, Linyan Zhu","doi":"10.3390/jof12100782","DOIUrl":"10.3390/jof12100782","url":null,"abstract":"<p><p><i>Rhizoctonia solani</i> Kühn is a destructive phytopathogenic fungus with an exceptionally broad host range, infecting over 32 plant families and 188 genera. In this study, we evaluated the antifungal activity of compound <b>1</b> from <i>Streptomyces</i> sp. F204 against <i>R. solani</i>, and characterized its genomic features and metabolomic responses under different culture conditions. Based on whole-genome sequences, the highest average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain F204 and its closest related type strain, <i>Streptomyces puniciscabiei</i> S77, were 91.64% and 45.00%, respectively. Compound <b>1</b>, the active metabolite isolated from the fermentation broth of <i>Streptomyces</i> sp. F204, was evaluated using the mycelial growth rate method. Genome annotation predicted 8022 genes in strain F204, including protein-coding genes, rRNA genes, and tRNA genes, covering 86.72% of the total genome length. Additionally, antiSMASH 6.0.0 analysis identified 23 biosynthetic gene clusters putatively involved in secondary metabolite production. Comparative metabolomics revealed significantly enriched pathways across different media, including nicotinate and nicotinamide metabolism in Medium No. 2 (B) versus Gause's synthetic medium (A), and pyrimidine metabolism as well as alanine, aspartate, and glutamate metabolism in ISP4 medium (C) versus Gause's synthetic medium (A). On the basis of NMR and mass spectrometry (MS) data, compound <b>1</b> is proposed to be a structural analog of daptomycin. It exhibited strong concentration-dependent inhibition of <i>R. solani</i> mycelial growth, achieving 93.07 ± 0.49% inhibition at 200 μg/mL, with an IC₅₀ value of 14.9 μg/mL. These findings indicate that <i>Streptomyces</i> sp. F204 represents a promising source of antifungal metabolites with considerable biosynthetic potential.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"12 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13705709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149268709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Updated Checklist of Cadaver-Associated Culturable Fungi, Along with the Isolation of Microfungi from a 70-Year-Old Male Cadaver.","authors":"Marco Iannaccone, Laura Canonica, Silvia Giusto, Grazia Cecchi, Simone Di Piazza, Rosario Barranco, Francesco Ventura, Mirca Zotti","doi":"10.3390/jof12100780","DOIUrl":"10.3390/jof12100780","url":null,"abstract":"<p><p>Forensic mycology investigates the role of fungi in human decomposition and may provide useful information on the postmortem interval (PMI), environmental conditions, and possible body movement. However, cadaver-associated fungal communities remain insufficiently investigated, particularly in terms of culturable taxa and their preservation in reference collections. To address this gap, we present an updated checklist of cadaver-associated culturable fungi spanning 1923-2026, compiled from 27 literature sources across 12 countries and comprising 883 records (879 from the literature and 4 from this study), including previously overlooked early 20th-century Italian records recovered from historical archives. The checklist includes four new isolates obtained from a 70-year-old male cadaver found in a basement in the putrefaction stage. Visible fungal growth was sampled from the left hand, left cheek, and right calf, cultured on Sabouraud and Rose Bengal media, and identified through morphological analysis and DNA barcoding: <i>Penicillium brevicompactum</i>, <i>Penicillium chrysogenum</i>, <i>Mucor saturninus</i>, and <i>Aspergillus versicolor</i>. Notably, <i>A. versicolor</i> was recovered from a cadaver in the putrefaction stage, expanding current knowledge of its forensic relevance. All isolates were deposited in the ColD UNIGE JRU MIRRI-IT collection, contributing to the development of forensic mycological databases.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"12 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13706631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149268753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TLR4 Regulates the Proinflammatory Response and Myeloperoxidase Activity in Neutrophils During Interaction with <i>Sporothrix schenckii</i>.","authors":"Yolanda Romo-Lozano, Aylin Mayela Avila-Colis, Daniel Cervantes-García, Rodolfo González-Segovia, Estela Ruiz-Baca","doi":"10.3390/jof12100779","DOIUrl":"10.3390/jof12100779","url":null,"abstract":"<p><p><i>Sporothrix schenckii</i> is a pathogenic fungus that triggers innate immune responses. Toll-like receptors (TLRs), such as TLR4, upon recognizing microbial components (including fungal ones), activate inflammatory and immune responses in cells. Meanwhile, in neutrophils, myeloperoxidase (MPO) is involved in oxidative mechanisms with antimicrobial activity. In this context, the aim of this study was to evaluate the role of TLR4 in the neutrophil response to <i>S</i>. <i>schenckii</i> and its potential relationship with MPO. To achieve this, human neutrophils, untreated or pre-treated with an anti-TLR4 antibody, were exposed for two hours to baseline conditions, lipopolysaccharide (LPS) stimulation, or <i>S</i>. <i>schenckii</i> conidia. Subsequently, viability, phagocytosis, MPO activity, and protein expression (TLR4, MPO) were assessed via Western blot. Additionally, gene expression (<i>TLR4</i>, <i>MPO</i>, <i>IL8</i>, <i>TNF</i>, <i>IL18</i>, <i>IL1B</i>) was evaluated using RT-qPCR, and IL-8 levels were quantified by ELISA. The results showed that <i>S</i>. <i>schenckii</i> conidia increased TLR4 and MPO expression in neutrophils. TLR4 blockade tended to reduce phagocytosis and fungal viability nonsignificantly while increasing <i>MPO</i> expression, altering cytokine production (increasing <i>IL8</i>, <i>IL18</i>, and <i>TNF</i>, and decreasing LPS-induced <i>IL1B</i>). These findings suggest that TLR4-mediated fungal recognition may modulate inflammation, MPO secretion, and cytokine release to control infection, highlighting its potential as an immunotherapeutic target.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"12 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13706431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149268895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accurate Extraction of Phenotypic Parameters for DUS Testing of <i>Oudemansiella raphanipes</i> Based on Deep Learning.","authors":"Tao Yuan, Dong Hu, Mengyuan Xu, Minglu Tian, Hailong Yu, Linyi Li","doi":"10.3390/jof12100778","DOIUrl":"10.3390/jof12100778","url":null,"abstract":"<p><p>To enable the rapid and accurate acquisition of phenotypic parameters during distinctness, uniformity, and stability (DUS) testing of edible mushroom varieties, and to address the low efficiency, strong subjectivity, and insufficient standardization of conventional manual measurement methods, this study used the fruiting bodies of <i>Oudemansiella raphanipes</i> as the research object and proposed an automatic phenotypic parameter extraction method based on deep learning instance segmentation. An instance-segmentation dataset containing 473 <i>O. raphanipes</i> images and 1419 annotated objects was constructed, and the YOLOv11s-seg model was improved by introducing the lightweight feature-extraction network StarNet_s050 to construct the YOLOv11s-star-seg model, thereby improving model inference efficiency and meeting practical application requirements. Under standardized imaging conditions, pileus and stipe regions were obtained from the instance-segmentation results, and image-scale calibration was combined to automatically extract DUS phenotypic parameters, including pileus diameter, pileus context thickness, stipe length, and stipe diameter, while quantitatively describing stipe shape. The experimental results showed that the YOLOv11s-star-seg model performed well in the instance segmentation of <i>O. raphanipes</i>, achieving an mAP50 of 99.1%, an F1 value of 98.9%, and an mIoU of 97.86%; compared with the YOLOv11s-seg model, the number of parameters was reduced by 39% and the inference time was reduced by 27%, thereby achieving model lightweighting while maintaining high segmentation accuracy. The automatically extracted results showed high consistency with the manual measurements; the coefficients of determination (R²) from linear regression fitting for stipe length, stipe diameter, and pileus diameter all exceeded 0.96, indicating that the method can effectively replace part of the manual measurement process. The results can provide technical support for the digitalization of DUS testing of <i>O. raphanipes</i>, intelligent breeding of edible mushrooms, and accurate phenotypic identification.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"12 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13705975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149268738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of Biotechnological Applications of <i>Glomeromycota</i>: Inoculum Production, Plant Performance, Environmental Management, and Metabolites.","authors":"Cícero Pinheiro Inácio, Greicilene Maria Rodrigues Albuquerque, Maria Alice Ribeiro Alves, Isabelly Guimarães Silva, Geuda Barbosa da Silva, Delson Laranjeira, Rejane Pereira Neves","doi":"10.3390/jof12100777","DOIUrl":"10.3390/jof12100777","url":null,"abstract":"<p><p>Arbuscular mycorrhizal fungi (<i>Glomeromycota</i>) offer routes to inoculum production, crop management, phytomanagement of contaminated soils, and modulation of plant metabolites. This critical narrative review integrates 102 selected original studies, including genomic, physiological, transcriptomic, cultivation, and field research. Evidence is interpreted by fungal strain, host genotype, tissue, environment, and application-specific endpoint. Host-lipid transfer and asymbiotic propagation inform inoculum manufacture, but spore production remains distinct from infective potency, storage stability, and economic feasibility. Receptor signaling and functional nutrient-transport studies connect symbiotic establishment to resource exchange; stress experiments reveal context-dependent changes in water transport, ion homeostasis, photosynthesis, and oxidative regulation. Commercial-product variability, resident microbiota, pesticide exposure, and ecological responses constrain translation. Metal localization and transport studies distinguish retention, mobilization, and tolerance from contaminant removal. Plant-metabolite experiments demonstrate chemical modulation rather than direct fungal biosynthesis or therapeutic efficacy. Soil carbon and hydraulic responses further show that ecological benefits cannot be generalized across communities or substrates. Development should prioritize verified inocula, defined host × fungal strain × environment combinations, relevant controls, and repeatable field outcomes. The narrative design and author-proposed assessment framework covers a deployment protocol.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"12 10","pages":""},"PeriodicalIF":4.3,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13706223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149268766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}