Journal of Fungi最新文献_第2页

Properties of Putative APSES Transcription Factor AfpA in Aspergillus fumigatus. 烟曲霉APSES转录因子AfpA的特性。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-16 DOI: 10.3390/jof11090678

Young-Ho Choi, Min-Woo Lee, Kwang-Soo Shin

引用次数: 0

Comparative Mitogenome Analysis of Colletotrichum Species Causing Anthracnose of Rubber Trees Unveils Distinct Species Complex-Specific Evolution Trajectories Within the Genus. 引起橡胶树炭疽病的炭疽菌种的比较有丝分裂基因组分析揭示了属内独特的物种复杂特异性进化轨迹。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-16 DOI: 10.3390/jof11090679

Yehao Wu, Fan Zhou, Qingqin Chen, Lijuan He, Yining Zang, Zirui Wang, Chunhua Lin, Weiguo Miao, Zhigang Li

{"title":"Comparative Mitogenome Analysis of Colletotrichum Species Causing Anthracnose of Rubber Trees Unveils Distinct Species Complex-Specific Evolution Trajectories Within the Genus.","authors":"Yehao Wu, Fan Zhou, Qingqin Chen, Lijuan He, Yining Zang, Zirui Wang, Chunhua Lin, Weiguo Miao, Zhigang Li","doi":"10.3390/jof11090679","DOIUrl":"10.3390/jof11090679","url":null,"abstract":"Colletotrichum spp. are the causative agents of anthracnose of rubber trees, one of the most destructive diseases, resulting in substantial economic losses. To investigate the evolutionary characteristics of these pathogenic species, we first assembled the complete mitogenomes of four dominant pathogens, i.e., C. siamense, C. fructicola, C. wanningense and C. bannaense. Comparative analyses revealed that variations in their mitogenome size were primarily driven by intron expansion and expansion/contraction within the cox1, cob and nad genes. Moreover, we observed the strong conservation of gene content, mitochondrial DNA copy number, gene order and intron features within species complexes, but a clear divergence between them. Notably, further studies indicated that patterns such as genomic organization, selective pressures and codon usage were consistent across the genus, suggesting that Colletotrichum species complexes had followed distinct evolutionary trajectories, particularly in the arrangement of protein-coding genes. Therefore, this study systematically characterized the mitogenomes of the four major Colletotrichum species associated with rubber tree anthracnose and provided novel insights into the broad evolutionary mechanisms shaping Colletotrichum species complexes.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470318/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

National Belgian Study on Terbinafine Resistance in Trichophyton interdigitale/mentagrophytes/indotineae (2022-2023): Epidemiology and Molecular Features. 比利时国家对特比萘芬抗性的研究（2022-2023）：流行病学和分子特征。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-13 DOI: 10.3390/jof11090676

Rosalie Sacheli, Sabrina Egrek, Khalid El Moussaoui, Bahoz Kurt, Emilie Machowski, Saadia Harag, Marie-Pierre Hayette

{"title":"National Belgian Study on Terbinafine Resistance in Trichophyton interdigitale/mentagrophytes/indotineae (2022-2023): Epidemiology and Molecular Features.","authors":"Rosalie Sacheli, Sabrina Egrek, Khalid El Moussaoui, Bahoz Kurt, Emilie Machowski, Saadia Harag, Marie-Pierre Hayette","doi":"10.3390/jof11090676","DOIUrl":"10.3390/jof11090676","url":null,"abstract":"Objectives: Trichophyton indotineae, a dermatophyte closely related to T. interdigitale and T. mentagrophytes, is of growing concern due to its high terbinafine resistance and widespread presence in India. Its emergence in Europe calls for enhanced surveillance. Resistance is linked to mutations in the squalene epoxidase (SQLE) gene. This multicentric national study aimed to evaluate the prevalence, terbinafine susceptibility, and phylogenomics of T. interdigitale/mentagrophytes/indotineae strains in Belgium, with a focus on SQLE substitutions. Methods: Between February 2022 and April 2023, 137 isolates from 16 Belgian labs were analyzed for antifungal susceptibility using the EUCAST E.Def.11.0 method. Whole-genome sequencing (WGS) was performed via Illumina sequencing method. Results: Phylogenomic analysis identified 8 T. indotineae, 91 T. interdigitale, and 38 T. mentagrophytes (including 7 genotype VII strains). Terbinafine resistance (5.1%) was mainly found in T. indotineae (87.5%), always linked to SQLE substitutions. T. interdigitale was fully susceptible. T. mentagrophytes showed mildly elevated MICs, often associated with K276N substitution. Conclusions: Terbinafine-resistant T. indotineae is emerging in Belgium, mostly via imported cases. Continued molecular surveillance and species-specific treatment strategies are essential.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and Characterization of the Mycovirus MpChrV2 from Macrophomina phaseolina. 菜绿巨噬菌分枝病毒MpChrV2的克隆与鉴定。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-12 DOI: 10.3390/jof11090675

Peimeng Sun, Luyang Song, Mengyuan Mu, Jiayi Ma, Xinyu Li, Kunni Tian, Mengyuan Zhang, Mingyue Zhang, Yuanyuan Zhang, Caiyi Wen, Jing Wang, Ying Zhao

{"title":"Cloning and Characterization of the Mycovirus MpChrV2 from Macrophomina phaseolina.","authors":"Peimeng Sun, Luyang Song, Mengyuan Mu, Jiayi Ma, Xinyu Li, Kunni Tian, Mengyuan Zhang, Mingyue Zhang, Yuanyuan Zhang, Caiyi Wen, Jing Wang, Ying Zhao","doi":"10.3390/jof11090675","DOIUrl":"10.3390/jof11090675","url":null,"abstract":"Macrophomina phaseolina is a widely distributed soilborne phytopathogenic fungus that causes destructive diseases such as charcoal rot and stem canker, posing serious threats to crop yield and quality. In recent years, mycoviruses have gained attention as potential biological control agents. In this study, a novel double-stranded RNA (dsRNA) virus was identified from M. phaseolina strain 22C-8, isolated from sesame (Sesamum indicum L.) charcoal rot samples in Fuyang, Anhui Province, China. The viral genome comprised four dsRNA segments, each encoding a single open reading frame (ORF) predicted to encode RNA-dependent RNA polymerase (RdRp), coat protein (CP), and two hypothetical proteins. Phylogenetic analysis classified the virus as a new member of the genus Betachrysovirus in the family Chrysoviridae, and it was designated Macrophomina phaseolina chrysovirus 2 (MpChrV2). Pathogenicity assays in sesame seedlings revealed that MpChrV2 infection significantly reduced the virulence of M. phaseolina strain 22C-8. In contrast, virus-free derivatives (22C-8-VF18), obtained via protoplast regeneration, caused more severe symptoms and exhibited enhanced growth rates, indicating that MpChrV2 alters fungal physiology and pathogenicity. These findings suggest that MpChrV2 possesses a typical hypovirulence phenotype and holds promise as a biocontrol agent for sesame charcoal rot.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics and Outcomes of Hospitalized Patients with Histoplasmosis: Comparison of Immunocompromised and Non-Immunocompromised Adult Patients. 组织胞浆菌病住院患者的特征和结局：免疫功能低下和非免疫功能低下成人患者的比较

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-12 DOI: 10.3390/jof11090671

Liam M Dalton, Carol A Kauffman, Marisa H Miceli

{"title":"Characteristics and Outcomes of Hospitalized Patients with Histoplasmosis: Comparison of Immunocompromised and Non-Immunocompromised Adult Patients.","authors":"Liam M Dalton, Carol A Kauffman, Marisa H Miceli","doi":"10.3390/jof11090671","DOIUrl":"10.3390/jof11090671","url":null,"abstract":"We sought to investigate the role of immunocompromise in patients with newly diagnosed histoplasmosis in an era when AIDS is less prevalent. We performed a retrospective comparison of immunocompromised and non-immunocompromised adults hospitalized at Michigan Medicine from 2015 to 2024. Of 51 patients, 37 (73%) were immunocompromised, 32 from solid organ transplantation or tumor necrosis factor antagonist/disease-modifying anti-rheumatic drugs. Of these 37, 34 had disseminated and 3 had pulmonary histoplasmosis; of the 14 non-immunocompromised patients, 8 had disseminated and 6 had pulmonary histoplasmosis, p = 0.004. Fever was the only symptom/sign that was more common in the immunocompromised cohort (86% vs. 36%, p = 0.003). Laboratory/radiological studies showed no major differences between immunocompromised and non-immunocompromised cohorts. Histoplasma urinary antigen was positive for all immunocompromised vs. 79% non-immunocompromised patients, p = 0.003. Median antigen levels were 17.5 (IQR 6.2-19.7) ng/mL for immunocompromised vs. 1.9 (0.6-19.7) ng/mL for non-immunocompromised patients, p = 0.004. Cultures for Histoplasma were more often positive in the immunocompromised cohort, p = 0.025. All-cause 90-day mortality was 14% in each cohort (five immunocompromised and two non-immunocompromised patients); all deaths occurred in those with disseminated histoplasmosis, and four were in the first week of hospitalization. Disseminated histoplasmosis in both immunocompromised and non-immunocompromised patients continues to be a serious, often fatal infection.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

T-DNA Mutagenesis Reveals FpPer1 as a Dual-Function Regulator of Virulence and Fungicide Resistance in Fusarium pseudograminearum. T-DNA突变揭示FpPer1是伪谷物镰刀菌毒力和杀菌剂抗性的双功能调节剂。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-12 DOI: 10.3390/jof11090673

Haiyang Li, Panpan Zhang, Xueqian Song, Huiying Li, Cong Chen, Limin Wang, Zhifang Wang, Lingjun Hao, Yun Li, Xinlong Wang, Jiangang Kang, Honglian Li, Min Wang, Shengli Ding

{"title":"T-DNA Mutagenesis Reveals FpPer1 as a Dual-Function Regulator of Virulence and Fungicide Resistance in Fusarium pseudograminearum.","authors":"Haiyang Li, Panpan Zhang, Xueqian Song, Huiying Li, Cong Chen, Limin Wang, Zhifang Wang, Lingjun Hao, Yun Li, Xinlong Wang, Jiangang Kang, Honglian Li, Min Wang, Shengli Ding","doi":"10.3390/jof11090673","DOIUrl":"10.3390/jof11090673","url":null,"abstract":"Fusarium crown rot (FCR), caused by Fusarium pseudograminearum, is a devastating wheat disease leading to significant yield losses worldwide. However, the pathogenic mechanism of F. pseudograminearum and its resistance to fungicides remain poorly understood. In this study, we identified a hypothetical gene encoding GPI-anchored protein, designated FpPer1, by screening a T-DNA insertion mutant library of F. pseudograminearum for tebuconazole resistance. The ΔFpper1 mutant exhibited increased sensitivity to the triazole antifungal drugs and fludioxonil. Additionally, the deletion of FpPER1 impaired fungal growth, conidiation, and pathogenicity in barley leaves and wheat coleoptiles. Furthermore, the ΔFpper1 mutant displayed enhanced susceptibility to various environmental stresses, including NaCl, CR, sorbitol, H2O2, and SDS. The mutant also showed reduced penetration peg formation and impaired reactive oxygen species (ROS) scavenging ability during infection. Subcellular localization analysis revealed that FpPer1-GFP co-localized with the endoplasmic reticulum (ER) marker RFP-HDEL in both conidia and hyphae, indicating its localization in the ER. In summary, our findings demonstrate that FpPER1 plays an important role in pathogenicity and fungicide resistance in F. pseudograminearum. This study not only provides a theoretical foundation for understanding fungal virulence mechanisms but also offers practical insights for developing novel fungicide strategies.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What Do We Know About Cryptococcus spp. in Portugal? One Health Systematic Review in a Comprehensive 13-Year Retrospective Study (2013-2025). 关于葡萄牙隐球菌我们知道些什么？一项综合13年回顾性研究（2013-2025）的健康系统评价。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-12 DOI: 10.3390/jof11090672

Ricardo Lopes, Andreia Garcês, Hugo Lima de Carvalho, Vanessa Silva, Filipe Sampaio, Cátia Fernandes, Gonçalo Barros, Alexandre Sardinha de Brito, Ana Rita Silva, Elsa Leclerc Duarte, Luís Cardoso, Ana Cláudia Coelho

{"title":"What Do We Know About Cryptococcus spp. in Portugal? One Health Systematic Review in a Comprehensive 13-Year Retrospective Study (2013-2025).","authors":"Ricardo Lopes, Andreia Garcês, Hugo Lima de Carvalho, Vanessa Silva, Filipe Sampaio, Cátia Fernandes, Gonçalo Barros, Alexandre Sardinha de Brito, Ana Rita Silva, Elsa Leclerc Duarte, Luís Cardoso, Ana Cláudia Coelho","doi":"10.3390/jof11090672","DOIUrl":"10.3390/jof11090672","url":null,"abstract":"Cryptococcosis, caused by the Cryptococcus neoformans and Cryptococcus gattii species complexes (pathogenic Cryptococcus spp.), is an environmentally acquired mycosis of One Health relevance. This study integrates a PRISMA-compliant systematic review (2000-2025) of Portuguese animal, human, and environmental reports with a 13-year retrospective dataset of laboratory-confirmed veterinary cryptococcosis cases (2013-2025). Clinical specimens were cultured and identified by MALDI-TOF mass spectrometry, and associations were assessed using χ2 and Fisher's exact tests. Of 1059 submissions, 48 (4.5%) were culture-positive: 6.8% of canine, 5.3% of feline samples, and 4.0% of avian samples, with no detections in other vertebrate groups (p = 0.705). Cryptococcus neoformans predominated in carnivores (73.7%), while Papiliotrema laurentii (formerly Cryptococcus laurentii) was most frequent in birds (86.2%). Infection was not associated with sex or age. Seasonality was evident, with a July peak and summer predominance (p = 0.010). Most cases were from the Centre region (62.5%), with significant regional variation of Cryptococcus spp. distribution (p < 0.001). The systematic review confirmed autochthonous C. gattii complex disease and widespread C. neoformans contamination in pigeon guano and arboreal niches. These findings demonstrate a compartmentalised eco-epidemiology, reinforcing the need for integrated molecular typing, antifungal susceptibility testing, and coordinated human-animal-environment surveillance to inform targeted prevention and control strategies in Portugal.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470549/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Transformation and Protein Expression of the Edible Mushroom Stropharia rugosoannulata Protoplasts by Agrobacterium-tumefaciens-Mediated Transformation. 农杆菌介导的食用菌原生质体转化及蛋白表达

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-12 DOI: 10.3390/jof11090674

Dongjie Yin, Hairong Xiong

{"title":"The Transformation and Protein Expression of the Edible Mushroom Stropharia rugosoannulata Protoplasts by Agrobacterium-tumefaciens-Mediated Transformation.","authors":"Dongjie Yin, Hairong Xiong","doi":"10.3390/jof11090674","DOIUrl":"10.3390/jof11090674","url":null,"abstract":"Stropharia rugosoannulata is a cultivated edible mushroom characterized by its nutritional composition and efficient cellulolytic enzymatic systems. However, the lack of genetic tools has significantly impeded the investigation of its molecular mechanisms, severely constraining the study of functional genomic and precision breeding in S. rugosoannulata. It was demonstrated in this study that the Agrobacterium-tumefaciens-mediated genetic transformation (ATMT) system is applicable for the transformation of S. rugosoannulata protoplasts. Through this proposal, we successfully achieved the expression of exogenous genes (mCherry gene encoding red fluorescent protein, hph gene encoding hygromycin B phosphotransferase, and GUS gene encoding β-glucuronidase) and the endogenous mutant gene SDI encoding the iron-sulfur protein subunit of succinate dehydrogenase in S. rugosoannulata. Furthermore, this study employed endogenous promoters of GPD encoding glyceraldehyde-3-phosphate dehydrogenase and SDI to enhance transformation efficiency and drive target gene expression. This study establishes the feasibility of ATMT in S. rugosoannulata systems, while achieving stable expression of a panel of selectable marker genes and reporter genes critical for genetic research in S. rugosoannulata.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface Changes Induced by Brushing Increase Candida albicans Biofilms on 3D-Printed Denture Base Resin. 刷牙引起的表面变化增加了3d打印义齿基托树脂上的白色念珠菌生物膜。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-12 DOI: 10.3390/jof11090668

Rafaelly Camargo, Jonatas Silva de Oliveira, Amanda Costa Ferro, Beatriz Ribeiro Ribas, Alan Augusto Valério Alves, Janaina Habib Jorge

{"title":"Surface Changes Induced by Brushing Increase Candida albicans Biofilms on 3D-Printed Denture Base Resin.","authors":"Rafaelly Camargo, Jonatas Silva de Oliveira, Amanda Costa Ferro, Beatriz Ribeiro Ribas, Alan Augusto Valério Alves, Janaina Habib Jorge","doi":"10.3390/jof11090668","DOIUrl":"10.3390/jof11090668","url":null,"abstract":"Studies assessing the long-term effects of brushing with cleaning and disinfecting agents on surface roughness and biofilm accumulation on the three-dimensionally (3D) printed resins remain scarce. This study aimed to evaluate the effects of different solutions and simulated brushing times on the surface roughness and biofilm formation on heat-cured and 3D-printed denture base resins. Discs samples (10 mm × 1.2 mm) were prepared and randomly assigned to brushing treatments using the following solutions: distilled water, disinfectant liquid soap and dentifrice. The specimens (n = 9) were subjected to 10,000, 20,000 and 50,000 cycles to simulate 1, 2 and 5 years of brushing, respectively. The control group consisted of unbrushed samples. Surface roughness (Ra) was measured. Candida albicans biofilm formation was evaluated by counting colony forming units, cellular metabolic activity, and qualitative and quantitative analysis through confocal fluorescence microscopy. A significant increase in roughness was observed in both resins after two years of simulated brushing, mainly with dentifrice. After two years of brushing, an increase in the number of cells and metabolism of C. albicans was observed, in agreement with the fluorescence and biofilm thickness results. Brushing with dentifrice increased the roughness of heat-cured and 3D-printed resins and potentially increased C. albicans biofilm formation.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Candidemia in a General Hospital in Kuwait: Epidemiology, Species Distribution, Risk Factors, and Antifungal Susceptibility Patterns over a 10-Year Period (2015-2024). 科威特一家综合医院的念珠菌病：10年间（2015-2024）的流行病学、种类分布、危险因素和抗真菌药敏模式

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-12 DOI: 10.3390/jof11090670

Khalifa Al Benwan, Sarah Ahmed, Dalal Al Banwan, Maria John

{"title":"Candidemia in a General Hospital in Kuwait: Epidemiology, Species Distribution, Risk Factors, and Antifungal Susceptibility Patterns over a 10-Year Period (2015-2024).","authors":"Khalifa Al Benwan, Sarah Ahmed, Dalal Al Banwan, Maria John","doi":"10.3390/jof11090670","DOIUrl":"10.3390/jof11090670","url":null,"abstract":"This 10-year retrospective observational study (2015-2024) conducted at Al-Amiri Hospital in Kuwait aimed to analyze the epidemiology, species distribution, and key risk factors associated with Candida bloodstream infections. Data were collected on patient demographics and clinical risk factors, and the distribution of Candida species was determined based on isolates recovered from patients with confirmed candidemia. Multivariate logistic regression was performed to identify factors associated with candidemia outcomes. Cases significantly increased from 33 (2015-2016) to 93 (2023-2024), predominantly affecting elderly patients (≥65 years) and intensive care unit (ICU) admissions. A shift in species distribution was observed, with a decline in Candida albicans and a marked increase in Candidozyma auris (formerly Candida auris) and C. parapsilosis. Antifungal susceptibility patterns were species-specific: C. albicans, C. parapsilosis, and C. tropicalis remained highly susceptible to all tested antifungals, while Nakaseomyces glabratus (formerly Candida glabrata) showed fluconazole resistance in 25% of isolates. C. auris exhibited resistance to fluconazole (97%) and variable resistance to echinocandins and voriconazole. Echinocandins retained broad-spectrum activity across most species. Independent risk factors included ICU admission, advanced age, and comorbidities. N. glabratus and C. auris infections were linked to higher mortality. This study highlights the growing candidemia burden in Kuwait, driven by emerging non-albicans Candida (NAC) spp. and related species. Early species identification and susceptibility testing are crucial for effective treatment and improved outcomes, necessitating enhanced infection control and antifungal stewardship.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0