Journal of Fungi最新文献_第3页

Diagnosis and Treatment of Superficial Fungal Infections. 浅表真菌感染的诊断与治疗。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-12 DOI: 10.3390/jof11090669

Suzana Otašević, Aleksandra Ignjatović

引用次数: 0

Virulence of Candida Isolates in Patients with Tuberculosis and Oral/Oesophageal Candidiasis: Co-Infection Evaluation. 念珠菌分离株在结核和口腔/食管念珠菌病患者中的毒力：联合感染评估。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-11 DOI: 10.3390/jof11090665

Rayana Larissa Pinheiro Soares Ferreira, Alessandra Teixeira Macedo, Conceição de Maria Pedrozo E Silva de Azevedo, Sirlei Garcia Marques, Marliete Carvalho Costa, João Carlos Maia Dornelas de Oliveira, Paulo Henrique Fonseca do Carmo, Yankee Costa Magalhães Diniz, Heylane Ferreira Cutrim, Cristina Andrade Monteiro, Maria Rosa Quaresma Bomfim, Daniel Assis Santos, Rodrigo Assuncao Holanda, Julliana Ribeiro Alves Santos

{"title":"Virulence of Candida Isolates in Patients with Tuberculosis and Oral/Oesophageal Candidiasis: Co-Infection Evaluation.","authors":"Rayana Larissa Pinheiro Soares Ferreira, Alessandra Teixeira Macedo, Conceição de Maria Pedrozo E Silva de Azevedo, Sirlei Garcia Marques, Marliete Carvalho Costa, João Carlos Maia Dornelas de Oliveira, Paulo Henrique Fonseca do Carmo, Yankee Costa Magalhães Diniz, Heylane Ferreira Cutrim, Cristina Andrade Monteiro, Maria Rosa Quaresma Bomfim, Daniel Assis Santos, Rodrigo Assuncao Holanda, Julliana Ribeiro Alves Santos","doi":"10.3390/jof11090665","DOIUrl":"10.3390/jof11090665","url":null,"abstract":"Tuberculosis (TB) is an infection caused by Mycobacterium tuberculosis complex (MTBC), which can be exacerbated by fungal infections. This study evaluated the clinical characteristics and virulence of Candida spp. in patients with tuberculosis. Antifungal sensitivity, phospholipase and proteinase production, biofilm formation, phagocytic index, and reactive oxygen (ROS) and nitrogen (RNS) species were assessed. Candida spp. were isolated from 14 patients, 28.5% women and 71.4% men, mainly from sputum and tracheal secretions. Five (35.7%) patients were co-infected with Mycobacterium, Candida, and HIV. Candida albicans (78.6%) and Candida tropicalis (21.4%) were identified in all 14 patients. All isolates showed sensitivity to amphotericin B and dose-dependent responses to fluconazole (16 μg/mL). Phospholipase activity was detected in 35.7% of the isolates, whereas all isolates showed proteinase activity (100%). A significant difference in phospholipase activity, phagocytosis, and production of reactive oxygen species (ROS) and nitrogen species (RNS) was observed when Candida isolates from patients with TB, living with or without HIV, were compared to Candida isolates from healthy individuals. All isolates were biofilm producers. This study highlights the relevance of mycoses diagnosis in patients with TB, since Candida spp. may be more virulent and contribute to the deterioration of the clinical condition.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery and Identification of Four Novel Species of Distoseptispora (Distoseptisporaceae, Distoseptisporales) on Decaying Wood from Hainan and Fujian Provinces, China. 海南和福建腐木上4个新菌种的发现和鉴定。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-11 DOI: 10.3390/jof11090667

Wenwen Liu, Changzhun Yin, Yang Jiang, Xigang Yan, Xingsheng Wang, Xiuguo Zhang, Shi Wang

{"title":"Discovery and Identification of Four Novel Species of Distoseptispora (Distoseptisporaceae, Distoseptisporales) on Decaying Wood from Hainan and Fujian Provinces, China.","authors":"Wenwen Liu, Changzhun Yin, Yang Jiang, Xigang Yan, Xingsheng Wang, Xiuguo Zhang, Shi Wang","doi":"10.3390/jof11090667","DOIUrl":"10.3390/jof11090667","url":null,"abstract":"Decaying wood, as a unique substrate, which contains cellulose, hemicellulose, lignin, and nitrogen-containing compounds, harbors significant fungal biodiversity, particularly among dematiaceous hyphomycete species. This study uses a combination of morphological and molecular systematics methods. Phylogenetic analyses of ITS, LSU, RPB2 and TEF1 sequences were conducted using the maximum likelihood (ML) and the Bayesian inference (BI) method. Combined with the morphological characteristics, four new species of Distoseptispora, D. bawanglingensis sp. nov., D. changjiangensis sp. nov., D. daanyuanensis sp. nov. and D. jianfenglingensis sp. nov., were identified from decaying wood collected in Hainan and Fujian provinces, China. This study provides detailed descriptions, illustrations, and phylogenetic trees with the aim of clarifying the taxonomic status of these four new species, thereby enhancing our understanding of the species diversity of Distoseptispora in Hainan and Fujian provinces, China.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can We Have Guidelines or Just Guidance for Rare Fungal Infections? 对于罕见的真菌感染，我们能有指南还是仅仅是指南？

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-11 DOI: 10.3390/jof11090666

Nancy N Vuong, Dimitrios P Kontoyiannis

引用次数: 0

The Use of Genetics and Immunology in the Diagnosis and Care of Advanced Coccidioidomycosis: Where Are We Going? 遗传学和免疫学在晚期球孢子菌病诊断和护理中的应用：我们要去哪里？

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-11 DOI: 10.3390/jof11090664

Kavitha Thiagarajan, Shikha Mishra, Rob Purdie, Bianca Torres, Royce H Johnson, Manish J Butte

{"title":"The Use of Genetics and Immunology in the Diagnosis and Care of Advanced Coccidioidomycosis: Where Are We Going?","authors":"Kavitha Thiagarajan, Shikha Mishra, Rob Purdie, Bianca Torres, Royce H Johnson, Manish J Butte","doi":"10.3390/jof11090664","DOIUrl":"10.3390/jof11090664","url":null,"abstract":"Coccidioidomycosis (CM) is an endemic disease in the western United States, northern Mexico, and Central and South America. The severity of coccidioidal infection is highly variable, with potential factors including comorbidities and the patient's innate and adaptive immune response. Based on data from a predominantly healthy and mainly Caucasian male survey conducted nearly a century ago, approximately 60% of infections are asymptomatic, with an estimated 40% of people experiencing some respiratory symptoms; with 10% of those diagnosed with CM. Disseminated disease occurs in approximately 1% of cases and can involve the meninges and, potentially, any place in the body. It is not yet fully understood why some people experience severe disease while many people do not; it is understood that the immune response has a major role. Immunomodulators, including dupilumab and interferon-gamma (IFN-γ), have shown promise in treating patients with disseminated infection. This article summarizes the latest genetic and immunologic evidence demonstrating immune dysfunction. Immunomodulators and potential therapeutic strategies based on the above are reviewed.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metagenomic Insights into Disease-Induced Microbial Dysbiosis and Elemental Cycling Alterations in Morchella Cultivation Soils: Evidence from Two Distinct Regions. 微生物生态失调和羊肚菌栽培土壤元素循环变化的宏基因组研究：来自两个不同地区的证据。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-10 DOI: 10.3390/jof11090663

Zong-Lin Deng, Feng-Ming Yu, Xiang Ma, Qi Zhao, Jian-Kui Liu

{"title":"Metagenomic Insights into Disease-Induced Microbial Dysbiosis and Elemental Cycling Alterations in Morchella Cultivation Soils: Evidence from Two Distinct Regions.","authors":"Zong-Lin Deng, Feng-Ming Yu, Xiang Ma, Qi Zhao, Jian-Kui Liu","doi":"10.3390/jof11090663","DOIUrl":"10.3390/jof11090663","url":null,"abstract":"Soil-borne diseases represent a major constraint on the sustainable cultivation of morel mushrooms (Morchella spp.), yet the microbial ecological mechanisms driving disease occurrence and progression remain poorly understood. In this study, we conducted comparative metagenomic analyses of rhizosphere and root-adhering soils associated with healthy and diseased Morchella crops from two major production regions in China, aiming to elucidate shifts in microbial community composition, assembly processes, and functional potential. Disease conditions were linked to pronounced microbial dysbiosis, with community assembly shifting from stochastic to deterministic processes, particularly within fungal communities under host selection and pathogen pressure. Co-occurrence network analysis revealed substantial reductions in connectivity, modularity, and clustering coefficients in diseased soils, indicating the loss of ecological stability and keystone taxa. Functional annotations using CAZy, COG, and KEGG databases showed that healthy soils were enriched in genes related to carbohydrate metabolism, aerobic respiration, and ecosystem resilience, whereas diseased soils exhibited higher abundance of genes associated with stress responses, proliferation, and host defense. Furthermore, elemental cycling analysis demonstrated that healthy soils supported pathways involved in aerobic carbon degradation, nitrogen fixation, phosphate transport, and sulfur oxidation, while diseased soils favored fermentation, denitrification, phosphorus limitation responses, and reductive sulfur metabolism. Collectively, these results highlight the importance of microbial functional integrity in maintaining soil health and provide critical insights into microbiome-mediated disease dynamics, offering a foundation for developing microbiome-informed strategies for sustainable fungal crop management.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Malassezia Folliculitis: An Underdiagnosed Mimicker of Acneiform Eruptions. 马拉色菌性毛囊炎：一种未被诊断为痤疮样疹的模仿者。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-10 DOI: 10.3390/jof11090662

Natalia V Chalupczak, Shari R Lipner

引用次数: 0

The Predicted Structure of S. cerevisiae Ssp1 Reveals Parallel Evolution in the Pil1 BAR Domain Family Proteins of Ascomycetes. 酿酒酵母Ssp1预测结构揭示子囊菌Pil1 BAR结构域家族蛋白平行进化

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-09 DOI: 10.3390/jof11090661

Yasuyuki Suda, Aaron M Neiman

{"title":"The Predicted Structure of S. cerevisiae Ssp1 Reveals Parallel Evolution in the Pil1 BAR Domain Family Proteins of Ascomycetes.","authors":"Yasuyuki Suda, Aaron M Neiman","doi":"10.3390/jof11090661","DOIUrl":"10.3390/jof11090661","url":null,"abstract":"BAR domains are a superfamily of widely conserved membrane binding motifs. In fungi, Pil1 family proteins are BAR domain containing proteins involved in organizing the plasma membrane. S. pombe encodes a sporulation-specific Pil1 family protein, Meu14, which has a specialized role in shaping the forespore membrane during sporulation. The functional analog of Meu14 in S. cerevisiae is Ssp1. While Ssp1 has no primary sequence homology to Pil1 or Meu14, AlphaFold predicts that it contains a Pil1-related BAR domain. Consistent with this structural prediction, mutation of residues in the putative lipid binding face of Ssp1 or in a residue implicated in multimerization disrupt sporulation. Characterization of the mutant proteins indicates that the BAR domain is necessary for recruitment of Ssp1 to the highly curved leading edge of the prospore membrane and multimerization of Ssp1 at that location is required for assembly of the leading edge complex. The distribution of Pil1 family proteins across an evolutionary tree of Ascomycetes reveals that Meu14 and Ssp1 arose independently in the lineages leading to S. pombe and S. cerevisiae, respectively.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Secreted Protein VdCUE Modulates Virulence of Verticillium dahliae Without Interfering with BAX-Induced Cell Death. 分泌蛋白VdCUE调节大丽花黄萎病毒力而不干扰bax诱导的细胞死亡。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-08 DOI: 10.3390/jof11090660

Haonan Yu, Haiyuan Li, Xiaochen Zhang, Mengmeng Wei, Xiaoping Hu, Jun Qin

{"title":"Secreted Protein VdCUE Modulates Virulence of Verticillium dahliae Without Interfering with BAX-Induced Cell Death.","authors":"Haonan Yu, Haiyuan Li, Xiaochen Zhang, Mengmeng Wei, Xiaoping Hu, Jun Qin","doi":"10.3390/jof11090660","DOIUrl":"10.3390/jof11090660","url":null,"abstract":"Verticillium wilt, caused by Verticillium dahliae, severely threatens various crops and trees worldwide. This study aimed to characterize the function of a CUE (coupling of ubiquitin conjugation to endoplasmic reticulum (ER) degradation)-domain-containing protein, VdCUE, in V. dahliae, which exhibits sequence divergence between the defoliating strain XJ592 and the non-defoliating strain XJ511. We generated ∆VdCUE-knockout mutants and evaluated their phenotypes in growth and virulence. Functional analyses included verifying the signal peptide activity of VdCUE, testing its ability to induce cell death or inhibit BAX-induced cell death in Nicotiana benthamiana leaves, and identifying host targets via yeast two-hybrid screening. The ∆VdCUE mutants showed reduced formation of melanized microsclerotia but no other obvious growth defects. Cotton plants infected with the ∆VdCUE mutants exhibited a significantly lower disease index and defoliation rate. VdCUE was confirmed to be secreted via a functional signal peptide, but it neither triggered cell death nor inhibited BAX-induced cell death. Three putative host targets were identified and supported by AI-based three-dimensional structural modeling, including tRNA-specific 2-thiouridylase, peptidyl-prolyl cis-trans isomerase, and 40S ribosomal protein, which may mediate VdCUE-dependent virulence regulation. These findings reveal VdCUE as a key virulence factor in V. dahliae, contributing to our understanding of its pathogenic mechanism.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Azole Resistance in Aspergillus Species Isolated from Clinical Samples: A Nine-Year Single-Center Study in Turkey (2015-2023). 从临床样本中分离的曲霉种对唑的耐药性：土耳其为期9年的单中心研究（2015-2023）。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-09-07 DOI: 10.3390/jof11090659

Zeynep Yazgan, Reyhan Çalışkan, Gökhan Aygün

{"title":"Azole Resistance in Aspergillus Species Isolated from Clinical Samples: A Nine-Year Single-Center Study in Turkey (2015-2023).","authors":"Zeynep Yazgan, Reyhan Çalışkan, Gökhan Aygün","doi":"10.3390/jof11090659","DOIUrl":"10.3390/jof11090659","url":null,"abstract":"Azole-resistant mycotic infections pose an escalating global health threat, with an estimated 6.5 million invasive fungal infections (IFIs) annually leading to 3.8 million deaths, 68% directly caused by IFIs. While azole antifungals are the cornerstone of treatment, emerging resistance, mainly due to gene mutations and efflux pump overexpression, is a major concern. This study, spanning 2015-2023, investigated azole resistance in clinical Aspergillus isolates in Turkey, a region lacking comprehensive surveillance. Of 200 causative isolates, A. fumigatus accounted for 45% and respiratory samples 57%. Overall azole resistance was 7%, rising to 11% for A. fumigatus. Findings highlight the persistent challenge of azole resistance, emphasizing the critical need for continued local and global surveillance to inform treatment guidelines and public health interventions. Despite limitations, including a single-center focus, this research provides crucial epidemiological insights into the evolving landscape of antifungal resistance in Turkey.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0