Journal of Fungi最新文献_第3页

The Biotherapeutic Potential of a Novel Probiotic Kluyveromyces marxianus Isolated from a Sourdough Starter Against Vaginal Candida albicans Strains.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-14 DOI: 10.3390/jof11020147

Annalisa Buonanno, Marianna Imparato, Angela Maione, Federica Carraturo, Emilia Galdiero, Marco Guida, Elisabetta de Alteriis

{"title":"The Biotherapeutic Potential of a Novel Probiotic Kluyveromyces marxianus Isolated from a Sourdough Starter Against Vaginal Candida albicans Strains.","authors":"Annalisa Buonanno, Marianna Imparato, Angela Maione, Federica Carraturo, Emilia Galdiero, Marco Guida, Elisabetta de Alteriis","doi":"10.3390/jof11020147","DOIUrl":"10.3390/jof11020147","url":null,"abstract":"There is an increasing interest in yeasts isolated from natural sources to be used as probiotics. Saccharomyces-based probiotics have been proposed as a valid alternative to the conventional drug therapy for the prevention and treatment of vulvovaginal candidiasis, also considering the resistance of some Candida strains to many antifungals. Here, we isolated from an artisanal sourdough a new yeast strain which was identified as Kluyveromyces marxianus and assessed its probiotic and safety properties, which resulted in comparable properties to all those exhibited by the commercial probiotic Saccharomyces boulardii. Then, we checked the antagonistic activity of the new isolate against some clinical fluconazole resistant C. albicans strains, showing its ability to inhibit filamentation, biofilm formation, and the adhesion of C. albicans to vaginal epithelial A-431 cells. Also, K. marxianus reduced the cell damage provoked by C. albicans and the expression of SAP2 and SAP6 genes. On the whole, our results enlarge the spectrum of the beneficial properties of the food-grade yeast K. marxianus showing for the first time its biotherapeutic potential against C. albicans.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Characterization of Three Epichloë Endophytes Isolated from Wild Barley in China.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-13 DOI: 10.3390/jof11020142

Zhengfeng Wang, Feng Zhao, Qijun Bao, Xiaoning Liu, Cheng Guo

{"title":"Identification and Characterization of Three Epichloë Endophytes Isolated from Wild Barley in China.","authors":"Zhengfeng Wang, Feng Zhao, Qijun Bao, Xiaoning Liu, Cheng Guo","doi":"10.3390/jof11020142","DOIUrl":"10.3390/jof11020142","url":null,"abstract":"Epichloë endophytes have been found in cool-season grasses and can produce alkaloids that are toxic to vertebrates and insects. Due to their beneficial effects, Epichloë can provide plants with resistance to some abiotic and biotic stresses. The biological and physiological characteristics of the endophytic strains XJE1, XJE2, and XJE3 isolated from wild barley were measured across a range of pH, salt concentrations, and growth values. The phylogenetic position of the Epichloë isolates was examined using the tefA and actG genes. The optimal pH values for mycelial growth of XJE1, XJE2, and XJE3 were 7-8, 6-7, and 8-9, respectively. The isolates grew significantly better at 0.3 mol/L NaCl than at 0.5 mol/L and 0.1 mol/L NaCl. Based on the conidiophore and conidia morphology, growth characteristics, and phylogenetic relationships, the endophyte isolated from wild barley is likely Epichloë bromicola. These isolates exhibited differences in mating types and alkaloid biosynthesis genes. Screening for salt tolerance and alkaloid biosynthetic genes in endophytic strains will provide new insights into useful traits to breed into new forage germplasms.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143491570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insight into the Mechanisms and Clinical Relevance of Antifungal Heteroresistance.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-13 DOI: 10.3390/jof11020143

Yanyu Su, Yi Li, Qiaolian Yi, Yingchun Xu, Tianshu Sun, Yingxing Li

{"title":"Insight into the Mechanisms and Clinical Relevance of Antifungal Heteroresistance.","authors":"Yanyu Su, Yi Li, Qiaolian Yi, Yingchun Xu, Tianshu Sun, Yingxing Li","doi":"10.3390/jof11020143","DOIUrl":"10.3390/jof11020143","url":null,"abstract":"Antifungal resistance poses a critical global health threat, particularly in immuno-compromised patients. Beyond the traditional resistance mechanisms rooted in heritable and stable mutations, a distinct phenomenon known as heteroresistance has been identified, wherein a minority of resistant fungal cells coexist within a predominantly susceptible population. Heteroresistance may be induced by pharmacological factors or non-pharmacological agents. The reversible nature of it presents significant clinical challenges, as it can lead to undetected resistance during standard susceptibility testing. As heteroresistance allows fungal pathogens to survive antifungal treatment, this adaptive strategy often leads to treatment failure and recurring infection. Though extensively studied in bacteria, limited research has explored its occurrence in fungi. This review summarizes the current findings on antifungal heteroresistance mechanisms, highlighting the clinical implications of fungal heteroresistance and the pressing need for deeper mechanism insights. We aim to bring together the latest research advances in the field of antifungal heteroresistance, summarizing in detail its known characteristics, inducing factors, molecular mechanisms, and clinical significance, and describing the similarities and differences between heteroresistance, tolerance and persistence. Further research is needed to understand this phenomenon and develop more effective antifungal therapies to combat fungal infections.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocontrol of Seedborne Fungi on Small-Grained Cereals Using Bacillus halotolerans Strain B33.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-13 DOI: 10.3390/jof11020144

Tatjana Popović Milovanović, Renata Iličić, Ferenc Bagi, Goran Aleksić, Nenad Trkulja, Vojislav Trkulja, Aleksandra Jelušić

{"title":"Biocontrol of Seedborne Fungi on Small-Grained Cereals Using Bacillus halotolerans Strain B33.","authors":"Tatjana Popović Milovanović, Renata Iličić, Ferenc Bagi, Goran Aleksić, Nenad Trkulja, Vojislav Trkulja, Aleksandra Jelušić","doi":"10.3390/jof11020144","DOIUrl":"10.3390/jof11020144","url":null,"abstract":"The development of biological pesticides is rapidly becoming an integral aspect of pest management in sustainable agriculture. This study was conducted to evaluate the effectiveness of Bacillus halotolerans strain B33 against three common seedborne fungal pathogens-Fusarium graminearum, Alternaria alternata, and Aspergillus flavus. B33 strain identity was determined using the 16S rRNA and tuf gene sequences. Commercial wheat, barley, oat, and rye seeds were artificially infected by fungal isolates and then treated with B33 overnight culture in Nutrient Broth. The obtained results indicate high efficacy against F. graminearum (83.55-94.38%) and A. alternata (85.05-96.70%), whereby the highest efficacy was noted on wheat seed and the lowest was detected on rye seed. On the other hand, B33 achieved 100% efficacy against A. flavus on barley, rye, and oat seeds, while being 96.24% effective against this pathogen on wheat. Principal component analysis indicated the highest treatment influence on A. flavus. The effect of all tested treatments on seed germination was statistically significant compared to the controls, whereby the number of germinated seeds declined as the seed infection rate increased. B. halotolerans strain B33 effectively managed seedborne fungal pathogens, thereby enhancing seed germination.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beauveria bassiana Induces Strong Defense and Increases Resistance in Tomato to Bemisia tabaci.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-13 DOI: 10.3390/jof11020141

Mengying Liu, Dong Xiang, Heikki M T Hokkanen, Tiandi Niu, Junjie Zhang, Jinlin Yang, Qiuyang Wei, Hanqiu Chen, Huai Liu, Yaying Li

{"title":"Beauveria bassiana Induces Strong Defense and Increases Resistance in Tomato to Bemisia tabaci.","authors":"Mengying Liu, Dong Xiang, Heikki M T Hokkanen, Tiandi Niu, Junjie Zhang, Jinlin Yang, Qiuyang Wei, Hanqiu Chen, Huai Liu, Yaying Li","doi":"10.3390/jof11020141","DOIUrl":"10.3390/jof11020141","url":null,"abstract":"Pre-stimulation of plants can change their resistance mechanisms, thereby enhancing their defense responses. Beauveria bassiana, a broad-spectrum entomogenous fungi, can also induce plant defenses, but it received little attention. Here, we show that B. bassiana can act as a stimulus to prime tomato defense responses, improving resistance in the plant to herbivore stress. The results illustrated that four defense genes (PIN2, PR2, PAL, and MPK3) were upregulated in all B. bassiana treatments, especially the phenylalanine deaminase (PAL) gene, which was highly expressed in tomato plants after B. bassiana inoculation. Feeding through Bemisia tabaci resulted in a weak upregulation of defense genes. However, in combined fungal inoculation and B. tabaci feeding, a total of nine defense genes were upregulated, among which five genes-PAL, PPO, PIN2, PR2, and PR1-were closely related to the phenol synthesis. The results of tomato plant metabolism showed that B. bassiana mainly activates tomato phenylpropane metabolic pathways, with this modulation being influenced by jasmonate. Further explorations revealed a significant enhancement in the antioxidant capacity of the plants, as evidenced by the determination of their antioxidant compounds and the coloration of leaf phenolic substances. Thus, entomopathogenic fungi can act as an exogenous substance to activate the defense responses of tomatoes without damaging the plant, indicating a good potential for developing applications using B. bassiana to promote resistance in tomatoes for pest management.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Differences Between Two Fusarium oxysporum Formae Speciales Causing Root Rot in Cucumber.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-12 DOI: 10.3390/jof11020140

Ernest Nailevich Komissarov, Roderic Gilles Claret Diabankana, Inna Abdeeva, Daniel Mawuena Afordoanyi, Sergey Vladimirovich Gudkov, Ekaterina Mikhailovna Dvorianinova, Sergey Alexandrovich Bruskin, Alexey Alexandrovich Dmitriev, Shamil Zavdatovich Validov

{"title":"Genomic Differences Between Two Fusarium oxysporum Formae Speciales Causing Root Rot in Cucumber.","authors":"Ernest Nailevich Komissarov, Roderic Gilles Claret Diabankana, Inna Abdeeva, Daniel Mawuena Afordoanyi, Sergey Vladimirovich Gudkov, Ekaterina Mikhailovna Dvorianinova, Sergey Alexandrovich Bruskin, Alexey Alexandrovich Dmitriev, Shamil Zavdatovich Validov","doi":"10.3390/jof11020140","DOIUrl":"10.3390/jof11020140","url":null,"abstract":"The host specificity of Fusarium oxysporum (Fox) formae speciales has been reported to be linked to effector proteins known as Secreted in Xylem (SIX). These genes are associated with the non-autonomous mobile element miniature impala (mimp), normally distributed on the accessory chromosomes. The pattern of mimp associated with effector genes has been used to predict candidate effector profiles which characterize Fox formae speciales. In this study, we demonstrate the pathogenicity of strains Fusarium oxysporum f.sp. radicis-lycopersici (Forl) ZUM2407 and Fusarium oxysporum f.sp. radicis-cucumerinum (Forc) V03-2g in a common host plant (cucumber) and compare their genomes. The Forl ZUM2407 genome lacks SIX genes and their homologs, in contrast to Forc V03-2g. We predicted the total number of mimp elements in the genome of Forl ZUM2407 to be three-fold less than that of Forc V03-2g (10 and 36 copies, respectively). The mimp distribution pattern in Forl ZUM2407 was completely different from that present in Forc V03-2g. Candidate effector profile analysis did not predict that Forl ZUM2407 was able to infect cucumber plants like Forc V03-2g. Therefore, we assume that Forl ZUM2407 has a different type of genome organization associated with pathogenicity, whose effector profile cannot be described using the mimp-based approach.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856433/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Inevitable Fate of Tetranychus urticae on Tomato Plants Treated with Entomopathogenic Fungi and Spinosad.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-12 DOI: 10.3390/jof11020138

Waqas Wakil, Maria C Boukouvala, Nickolas G Kavallieratos, Aqsa Naeem, Dionysios Ntinokas, Muhammad Usman Ghazanfar, Pasco B Avery

{"title":"The Inevitable Fate of Tetranychus urticae on Tomato Plants Treated with Entomopathogenic Fungi and Spinosad.","authors":"Waqas Wakil, Maria C Boukouvala, Nickolas G Kavallieratos, Aqsa Naeem, Dionysios Ntinokas, Muhammad Usman Ghazanfar, Pasco B Avery","doi":"10.3390/jof11020138","DOIUrl":"10.3390/jof11020138","url":null,"abstract":"Tetranychus urticae (Acari: Tetranychidae) is a pervasive and damaging mite pest of tomato crops, leading to important economic losses globally. This study evaluated the acaricidal efficacy of spinosad, alone and in combination with Beauveria bassiana (Bb) WG-21 and Metarhizium robertsii (Mr) WG-04, in the laboratory (application to tomato leaf discs) and greenhouse (application to tomato plants), considering mortality and establishment, respectively. The combination treatments of Bb WG-21 or Mr WG-04 with spinosad achieved 100% mortality of T. urticae nymphs within 2 days on leaf discs, while individual applications of each control agent resulted in lower mortalities, ranging between 62.91 and 86.25% after 3 days. The paired treatment of Mr WG-04 + spinosad killed all exposed adults within 5 d, while that of Bb WG-21 + spinosad achieved the same results after 7 d. However, spinosad, Mr WG-04, and Bb WG-21 alone killed ≥77.08% of adults after 7 d. In the greenhouse, the combination treatment of WG-04 + spinosad deterred the presence of T. urticae (adults, immatures, and eggs) on either surface of the tomato leaves, while Bb WG-21 + spinosad suppressed the populations only on the adaxial surface. These findings indicate that combined treatments of the tested EPF + spinosad, especially Mr WG-04, on tomato plants under greenhouse conditions can provide substantially enhanced control of T. urticae life stages compared to each treatment applied alone.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New Species of Ascomycetes from Two Hypersaline Endorheic Lagoon Complexes in Zaragoza Province (Aragon Community, Spain).

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-12 DOI: 10.3390/jof11020139

María Barnés-Guirado, José F Cano-Lira, Andrew N Miller, Alberto M Stchigel

{"title":"New Species of Ascomycetes from Two Hypersaline Endorheic Lagoon Complexes in Zaragoza Province (Aragon Community, Spain).","authors":"María Barnés-Guirado, José F Cano-Lira, Andrew N Miller, Alberto M Stchigel","doi":"10.3390/jof11020139","DOIUrl":"10.3390/jof11020139","url":null,"abstract":"Although certain hypersaline endorheic lagoons in Spain have been investigated to evaluate the composition, spatial structure, and ecological roles of their macro- and microbiota, the fungi inhabiting these niches remain largely unexplored. In this study, we isolated several microfungi from the Salada Grande de Chiprana and La Playa lagoons, located in the Saladas de Chiprana (Directed Natural Reserve and Ramsar Wetland) and the Saladas de Sástago-Bujaraloz (Ramsar Wetland), respectively. As a result of morphological characterization and phylogenetic analysis using four informative molecular markers, we report the discovery of two new species of the genus Montagnula (M.), M. globospora and M. terricola, as well as one new species of Monosporascus (Mo.), Mo. auratispora. Montagnula globospora, isolated from a sediment sample from Salada Grande de Chiprana lagoon, is the only species of the genus producing unicellular, globose ascospores inside cleistothecial ascomata with a cephalothecoid peridium. Montagnula terricola was originally isolated from a soil sample in Malawi (ex-type strain). However, we have also identified another strain of this species from a sediment sample collected at La Playa lagoon. The remarkable features of M. terricola are the production of non-cephalothecoid cleistothecial ascomata and bicellular, bi-cupulate ascospores. Regarding Mo. auratispora, it was isolated from sediments of Salada Grande de Chiprana and is characterized by the production of golden-brown ascospores that do not turn black with age. Also, due to the results of our phylogenetic analysis, we transferred Herpotrichia striatispora to the genus Montagnula, as M. striatispora, and Montangula jonessi to the new genus Neomontagnula (N.), as N. jonessi.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetically Improved Yeast Strains with Lower Ethanol Yield for the Wine Industry Generated Through a Two-Round Breeding Program.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-11 DOI: 10.3390/jof11020137

Eduardo I Kessi-Pérez, Melissa Gómez, William Farías, Verónica García, María Angélica Ganga, Amparo Querol, Claudio Martínez

{"title":"Genetically Improved Yeast Strains with Lower Ethanol Yield for the Wine Industry Generated Through a Two-Round Breeding Program.","authors":"Eduardo I Kessi-Pérez, Melissa Gómez, William Farías, Verónica García, María Angélica Ganga, Amparo Querol, Claudio Martínez","doi":"10.3390/jof11020137","DOIUrl":"10.3390/jof11020137","url":null,"abstract":"Saccharomyces cerevisiae is a species of industrial significance in the production of alcoholic beverages; it is the main species responsible for the fermentation of grape must. One of the main current problems in the wine industry is high alcohol levels caused by climate change. Pre- and post-fermentation strategies are used to reduce the alcohol content in wines; however, they are inefficient, affect organoleptic properties, face legal restrictions, and/or increase production costs, which has motivated efforts to obtain microbiological solutions. In the present work, we carried out a two-round breeding program to obtain improved yeast strains with lower ethanol yield. The trait under study showed high heritability (0.619), and we were able to lower the ethanol yield by 10.7% in just one generation. We finally obtained a population composed of 132 strains, of which 6 were used to produce wine from natural grape musts on a pilot scale, highlighting improved strains \"C2-1B4\" and \"C7-1B7\" as those that showed the best results (alcohol levels between 0.3 and 1.5% ABV less than expected). Further studies are required to understand the connection between initial sugar concentration and ethanol yield, as well as the genetic variants underlying this phenotype.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome Sequencing, Assembly, and Characterization of Cyberlindnera rhodanensis J52 as a Non-Saccharomyces Yeast with Ester-Enhancing Potential.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-02-11 DOI: 10.3390/jof11020135

Yong Shen, Zongcai Tu, Lizhou Tang, Yiyong Luo

{"title":"Genome Sequencing, Assembly, and Characterization of Cyberlindnera rhodanensis J52 as a Non-Saccharomyces Yeast with Ester-Enhancing Potential.","authors":"Yong Shen, Zongcai Tu, Lizhou Tang, Yiyong Luo","doi":"10.3390/jof11020135","DOIUrl":"10.3390/jof11020135","url":null,"abstract":"Cyberlindnera rhodanensis J52, a non-Saccharomyces yeast isolated from edible roses, markedly improves the organoleptic qualities of fermented foods. To facilitate the development and application of this strain, this study sequenced and assembled the genome of C. rhodanensis J52, subsequently conducting functional annotation of its genes utilizing the NR, Swiss-Prot, COG, GO, KEGG and CAZy databases. The findings revealed that this yeast harbors genes involved in the biosynthesis of flavor compounds, including higher alcohols, acetate esters, ethyl esters, volatile organic acids, aromatic amino acids and benzyl alcohol or benzaldehyde. Furthermore, it possesses β-glucosidase, an extracellular enzyme which enhances the flavor profile of fermented products. Further analysis revealed that the yeast features biosynthetic pathways for the production of isoamyl acetate, isoamyl 2-methylbutyrate, benzyl acetate, phenethyl acetate, ethyl butanoate and ethyl decanoate, which verifies its ability to produce esters at the genetic level. Additionally, the yeast was found to have the capacity to biosynthesize selenoproteins, suggesting that it not only enhances flavor but also imparts functional benefits. These findings provide a theoretical foundation for the further exploration and application of C. rhodanensis J52.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 2","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0