Journal of Fungi最新文献_第3页

A New Genus Neotricholomopsis Gen. Nov and Description of Neotricholomopsis globispora Sp. Nov. (Phyllotopsidaceae, Agaricales) from Northwestern China Based on Phylogeny, Morphology, and Divergence Time. 基于系统发育、形态和分化时间的中国西北地区姬松茸科 Neotricholomopsis Gen.基于系统发育、形态学和分化时间的中国西北地区姬松茸科（Phyllotopsidaceae, Agaricales）新属Neotricholomopsis Gen Nov和Neotricholomopsis globispora Sp.

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2024-11-13 DOI: 10.3390/jof10110784

Longfei Fan, Biyue Wang, Xue Zhong, Hongmin Zhou, Shunyi Yang, Xiaohong Ji

{"title":"A New Genus Neotricholomopsis Gen. Nov and Description of Neotricholomopsis globispora Sp. Nov. (Phyllotopsidaceae, Agaricales) from Northwestern China Based on Phylogeny, Morphology, and Divergence Time.","authors":"Longfei Fan, Biyue Wang, Xue Zhong, Hongmin Zhou, Shunyi Yang, Xiaohong Ji","doi":"10.3390/jof10110784","DOIUrl":"10.3390/jof10110784","url":null,"abstract":"While investigating macrofungi diversity in Gansu province, northwestern China, five fresh and fleshy specimens were collected, which are characterized by nearly white to buff hemispherical pileus with waved margins, a disc depressed with coral to brownish red fibrillose scales, adnate to sub-decurrent lamellae with four relatively regular rows of lamellulae, a stipe that is central, hollow, frequently straight to curved; basidiospores that are globose to subglobose, 5.0-6.0 × (3.5-) 4.0-5.0 (-5.5) μm, narrowly clavate cheilocystidia predominantly, pleurocystidia and caulocystidia not observed; and a cutis pileipellis, with hyphae slightly inflated in the KOH. The results of phylogeny analysis indicated that the species forms an independent lineage in Phyllotopsidaceae based on the ITS (ITS5/ITS4) and nLSU (LR0R/LR7) dataset. Molecular clock analyses suggested the common ancestor of Neotricholomopsis emerged later than upper Cretaceous with a mean crown age of 229.36 Mya (95% highest posterior density of 129.63-343.08 Mya). These five specimens were described as an unreported taxon based on the phylogeny analysis combined with morphological examination and ecological and geographical distribution. Detailed descriptions, illustrations, and phylogenetic trees to demonstrate the placement of this species and discussions with its related species are provided.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of COVID-19 on Antifungal Prescribing in the UK-Lessons to Learn. COVID-19 对英国抗真菌药物处方的影响--值得借鉴的经验。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2024-11-13 DOI: 10.3390/jof10110787

Katharine Pates, Zhendan Shang, Rebeka Jabbar, Darius Armstrong-James, Silke Schelenz, Jimstan Periselneris, Rossella Arcucci, Anand Shah

{"title":"The Effects of COVID-19 on Antifungal Prescribing in the UK-Lessons to Learn.","authors":"Katharine Pates, Zhendan Shang, Rebeka Jabbar, Darius Armstrong-James, Silke Schelenz, Jimstan Periselneris, Rossella Arcucci, Anand Shah","doi":"10.3390/jof10110787","DOIUrl":"10.3390/jof10110787","url":null,"abstract":"Fungal infections are increasingly prevalent; however, antifungal stewardship attracts little funding or attention. Previous studies have shown that knowledge of guidelines and scientific evidence regarding antifungals is poor, leading to prescribing based on personal experiences and the inherent biases this entails. We carried out a retrospective study of inpatient antifungal usage at two major hospitals. We assessed the longitudinal trends in antifungal usage and the effect of COVID-19 on antifungal prescription, alongside levels of empirical and diagnostically targeted antifungal usage. Our results showed that the longitudinal patterns of total systemic antifungal usage within the trusts were similar to national prescribing trends; however, the composition of antifungals varied considerably, even when looking exclusively at the more homogenous group of COVID-19 patients. We showed a high level of empirical antifungal use in COVID-19 patients, with neither trust adhering to international recommendations and instead appearing to follow prior prescribing habits. This study highlights the significant challenges to optimise antifungal use with prescribing behaviour largely dictated by habit, a lack of adherence to guidelines, and high rates of empirical non-diagnostic-based prescribing. Further research and resources are required to understand the impact of antifungal stewardship on improving antifungal prescribing behaviours in this setting and the effects on outcome.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595319/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Algerian Soil-Living Streptomyces alboflavus Strain as Source of Antifungal Compounds for the Management of the Pea Pathogen Fusarium oxysporum f. sp. pisi. 阿尔及利亚土壤生物链霉菌（Streptomyces alboflavus）菌株是防治豌豆病原体 Fusarium oxysporum f. sp. pisi 的抗真菌化合物来源。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2024-11-12 DOI: 10.3390/jof10110783

Marco Masi, Dorsaf Nedjar, Moustafa Bani, Ivana Staiano, Maria Michela Salvatore, Karima Khenaka, Stefany Castaldi, Jesus Garcia Zorrilla, Anna Andolfi, Rachele Isticato, Alessio Cimmino

{"title":"An Algerian Soil-Living Streptomyces alboflavus Strain as Source of Antifungal Compounds for the Management of the Pea Pathogen Fusarium oxysporum f. sp. pisi.","authors":"Marco Masi, Dorsaf Nedjar, Moustafa Bani, Ivana Staiano, Maria Michela Salvatore, Karima Khenaka, Stefany Castaldi, Jesus Garcia Zorrilla, Anna Andolfi, Rachele Isticato, Alessio Cimmino","doi":"10.3390/jof10110783","DOIUrl":"10.3390/jof10110783","url":null,"abstract":"Fusarium wilt caused by Fusarium oxysporum f. sp. pisi (Fop) poses significant threats to pea cultivation worldwide. Controlling this disease is mainly achieved through the integration of various disease management procedures, among which biological control has proven to be a safe and effective approach. This study aims to extract and identify antifungal secondary metabolites from Streptomyces alboflavus KRO3 strain and assess their effectiveness in inhibiting the in vitro growth of Fop. This bacterial strain exerts in vitro antagonistic activity against Fop, achieving highly significant inhibition over one week. The ethyl acetate extract, obtained from its ISP2 agar medium culture, also exhibited strong antifungal activity, maintaining an inhibition rate of approximately 90% at concentrations up to 250 µg/plug compared to the control. Thus, the organic extract has been fractionated using chromatographic techniques and its bioguided purification allowed us to isolate the main bioactive compound. This latter was identified as metacycloprodigiosin using nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and specific optical rotation data. Metacycloprodigiosin demonstrates dose-dependent inhibitory activity against the phytopathogen with an effective concentration of 125 µg/plug. The other secondary metabolites present in the ethyl acetate extract were also identified by gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR). This study highlighted the potential of S. alboflavus KRO3 strain and its antimicrobial compounds for the management of the pea pathogen Fusarium oxysporum f. sp. pisi.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Key Amino Acid Residues of the Agt1 Transporter for Trehalose Transport by Saccharomyces cerevisiae. 麦角酵母转运吸海盐的 Agt1 转运体的关键氨基酸残基

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2024-11-11 DOI: 10.3390/jof10110781

Anqi Chen, Yuhan Cheng, Liushi Meng, Jian Chen

{"title":"Key Amino Acid Residues of the Agt1 Transporter for Trehalose Transport by Saccharomyces cerevisiae.","authors":"Anqi Chen, Yuhan Cheng, Liushi Meng, Jian Chen","doi":"10.3390/jof10110781","DOIUrl":"10.3390/jof10110781","url":null,"abstract":"Trehalose is crucial for the stress resistance of Saccharomyces cerevisiae, primarily through its stabilization of proteins and membranes. The Agt1 transporter, a member of the Major Facilitator Superfamily, mediates trehalose uptake, a key process for maintaining cellular integrity under stress. Despite its importance, the molecular mechanisms of Agt1-mediated trehalose transport remain underexplored. In this study, we expressed and purified the trehalase enzyme TreA from E. coli to develop reliable trehalose assays. We screened 257 wild S. cerevisiae isolates, identifying strains with enhanced trehalose transport capacities. Comparative analyses, including structural modeling and molecular docking, revealed that specific Agt1 variants exhibited significantly higher transport efficiency, influenced by key residues in the transporter. Molecular dynamics simulations and steered molecular dynamics provided further insights, particularly into the role of the Agt1 channel head region in substrate recognition and binding. Site-directed mutagenesis validated these findings, showing that mutations at critical residues, such as 156Q, 164L, 256Q, 395E, 396R, and 507Y significantly reduced transport activity, while 137Q, 230T, and 514 N increased efficiency under certain conditions.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595304/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases. 金属纳米粒子对引起柠檬采后主要病害的抗真菌病原体的抗真菌作用。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2024-11-11 DOI: 10.3390/jof10110782

Carina G Baigorria, Luciana Cerioni, Mario A Debes, Ana E Ledesma, Patricio Alastuey, Mónica Tirado, Sabrina I Volentini, Viviana A Rapisarda

{"title":"Antifungal Action of Metallic Nanoparticles Against Fungicide-Resistant Pathogens Causing Main Postharvest Lemon Diseases.","authors":"Carina G Baigorria, Luciana Cerioni, Mario A Debes, Ana E Ledesma, Patricio Alastuey, Mónica Tirado, Sabrina I Volentini, Viviana A Rapisarda","doi":"10.3390/jof10110782","DOIUrl":"10.3390/jof10110782","url":null,"abstract":"Postharvest fungal diseases are the main cause of economic losses in lemon production. The continued use of synthetic fungicides to control the diseases favors the emergence of resistant strains, which encourages the search for alternatives. The aim of this study was to assess the efficacy of metallic nanoparticles (NPs) as antifungal agents against local isolates of Penicillium digitatum and Penicillium italicum, each of them in a fungicide-sensitive and -resistant version, and a Geotrichum citri-aurantii isolate. NPs of ZnO, CuO, and Ag were synthesized and characterized by spectroscopy and microscopy, presenting average sizes < 25 nm and spherical shapes. ZnO-NPs did not present antifungal activity at the assayed conditions, while the minimum fungicidal concentrations (MFCs) were 1000 and 10 µg mL-1 for CuO-NPs and Ag-NPs, respectively. The NPs' antimicrobial action included conidial membrane permeability and strong intracellular disorganization. Moreover, the Ag-NPs reduced green mold incidence on inoculated lemons when applied to the fruit. Taken together, Ag-NPs were effective in inhibiting both fungicide-sensitive and -resistant isolates of the main lemon postharvest pathogens, suggesting their potential use as an alternative approach.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595958/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatiotemporal Pattern of a Macrofungal Genus Phylloporia (Basidiomycota) Revealing Its Adaptive Evolution in China. 大型真菌 Phylloporia 属（担子菌纲）的时空模式揭示其在中国的适应性演化

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2024-11-10 DOI: 10.3390/jof10110780

Xue-Wei Wang, Li-Wei Zhou

{"title":"Spatiotemporal Pattern of a Macrofungal Genus Phylloporia (Basidiomycota) Revealing Its Adaptive Evolution in China.","authors":"Xue-Wei Wang, Li-Wei Zhou","doi":"10.3390/jof10110780","DOIUrl":"10.3390/jof10110780","url":null,"abstract":"The understanding of distribution and the evolutionary scenario is crucial for the utilization and conservation of biological resources; nevertheless, such explorations rarely focus on macrofungi. The current study selects a macrofungal genus, Phylloporia, and explores its spatiotemporal pattern in China. A total of 117 available occurrence records of Phylloporia in China were summarized for the current analyses. Ensemble modeling supports the highly suitable habitat of Phylloporia concentrated in southern, especially southeastern, China, where the ancestor of Phylloporia originated 77.74 million years ago and then dispersed to other parts of China. Benefitting from the available suitable habitats, Phylloporia rapidly diversified after its divergence in Southeast China. Then, the net diversification rate slowed down when the rapidly diversifying species filled available niches in Southeast China and the dispersed species in other parts of China inhabited the less suitable and unsuitable habitats. During adaptive evolution, precipitation, temperature and the host plant are the major environmental variables that shape the spatiotemporal pattern of Phylloporia. In conclusion, the current study reveals the adaptive evolutionary scenario of Phylloporia and provides the first exploration of the spatiotemporal pattern of macrofungi.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative Monitoring of Cyclic Glycine-Proline in Marine Mangrove-Derived Fungal Metabolites. 海洋红树林衍生真菌代谢物中环甘氨酸-脯氨酸的定量监测。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2024-11-10 DOI: 10.3390/jof10110779

Jing Lin, Fei Qin, Zeye Lin, Weijian Lin, Minxin You, Li Xu, Lei Hu, Yung-Husan Chen

引用次数: 0

Recent Updates on the Secondary Metabolites from Fusarium Fungi and Their Biological Activities (Covering 2019 to 2024). 镰刀菌次生代谢物及其生物活性的最新进展（2019 年至 2024 年）。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2024-11-09 DOI: 10.3390/jof10110778

Prosper Amuzu, Xiaoqian Pan, Xuwen Hou, Jiahang Sun, Muhammad Abubakar Jakada, Eromosele Odigie, Dan Xu, Daowan Lai, Ligang Zhou

{"title":"Recent Updates on the Secondary Metabolites from Fusarium Fungi and Their Biological Activities (Covering 2019 to 2024).","authors":"Prosper Amuzu, Xiaoqian Pan, Xuwen Hou, Jiahang Sun, Muhammad Abubakar Jakada, Eromosele Odigie, Dan Xu, Daowan Lai, Ligang Zhou","doi":"10.3390/jof10110778","DOIUrl":"10.3390/jof10110778","url":null,"abstract":"Fusarium species are commonly found in soil, water, plants, and animals. A variety of secondary metabolites with multiple biological activities have been recently isolated from Fusarium species, making Fusarium fungi a treasure trove of bioactive compounds. This mini-review comprehensively highlights the newly isolated secondary metabolites produced by Fusarium species and their various biological activities reported from 2019 to October 2024. About 276 novel metabolites were revealed from at least 21 Fusarium species in this period. The main metabolites were nitrogen-containing compounds, polyketides, terpenoids, steroids, and phenolics. The Fusarium species mostly belonged to plant endophytic, plant pathogenic, soil-derived, and marine-derived fungi. The metabolites mainly displayed antibacterial, antifungal, phytotoxic, antimalarial, anti-inflammatory, and cytotoxic activities, suggesting their medicinal and agricultural applications. This mini-review aims to increase the diversity of Fusarium metabolites and their biological activities in order to accelerate their development and applications.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three New Species and Five New Host Records from Chaetomiaceae with Anti-Phytopathogenic Potential from Cover Crops Astragalus sinicus and Vicia villosa. 具有抗病原菌潜能的茶黄菊科（Chaetomiaceae）3个新种和5个新宿主记录，产自黄芪（Astragalus sinicus）和紫云英（Vicia villosa）。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2024-11-08 DOI: 10.3390/jof10110776

Ning Qian, Yuhong Wu, Wei Zhang, Jun Yang, Vijai Bhadauria, Guozhen Zhang, Jiye Yan, Wensheng Zhao

{"title":"Three New Species and Five New Host Records from Chaetomiaceae with Anti-Phytopathogenic Potential from Cover Crops Astragalus sinicus and Vicia villosa.","authors":"Ning Qian, Yuhong Wu, Wei Zhang, Jun Yang, Vijai Bhadauria, Guozhen Zhang, Jiye Yan, Wensheng Zhao","doi":"10.3390/jof10110776","DOIUrl":"10.3390/jof10110776","url":null,"abstract":"Cover crops, typically planted during off-seasons and requiring less agronomic manipulation, may provide abundant fungal resources. Certain species of Chaetomiaceae could serve as potential agents for controlling plant diseases and developing bioorganic fertilizers. Eight species from five genera of Chaetomiaceae were identified from healthy Astragalus sinicus and Vicia villosa, two major cover crops, through multigene phylogenetic analysis, morphological identification, and pairwise homoplasy index testing. The identified species comprise three new species: Achaetomium astragali, Subramaniula henanensis, and S. sichuanensis, as well as five known but new host record species: Botryotrichum murorum, Chaetomium coarctatum, C. pseudocochliodes, C. pseudoglobosum, and Collariella pachypodioides. Dual culture tests revealed that isolates of all eight Chaetomiaceae species exhibited antagonistic effects on multiple phytopathogens. Among the identified fungi, the NSJA2 isolate, belonging to C. coarctatum, exhibited significant relative inhibition effects on 14 out of 15 phytopathogens tested in this study, indicating its broad-spectrum antagonistic effects. Additionally, NSJA2 exhibited excellent salt tolerance. Overall, our study has identified multiple fungi with anti-phytopathogens potential, among which NSJA2 exhibits high potential for practical application. This finding paves the way for further exploration and exploitation of NSJA2 as a promising biocontrol agent.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595803/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the Potential of Yarrowia lipolytica to Utilize Waste Motor Oil as a Carbon Source. 揭示脂肪分解酵母菌利用废机油作为碳源的潜力。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2024-11-08 DOI: 10.3390/jof10110777

Sílvia M Miranda, Isabel Belo, Marlene Lopes

{"title":"Unraveling the Potential of Yarrowia lipolytica to Utilize Waste Motor Oil as a Carbon Source.","authors":"Sílvia M Miranda, Isabel Belo, Marlene Lopes","doi":"10.3390/jof10110777","DOIUrl":"10.3390/jof10110777","url":null,"abstract":"This study evaluated the potential of Y. lipolytica (CBS 2075 and DSM 8218) to grow in waste motor oil (WMO) and produce valuable compounds, laying the foundation for a sustainable approach to WMO management. Firstly, yeast strains were screened for their growth on WMO (2-10 g·L-1) in microplate cultures. Despite limited growth, the CBS 2075 strain exhibited comparable growth to control conditions (without WMO), while DSM 8218 growth increased 2- and 3-fold at 5 g·L-1 and 10 g·L-1 WMO, respectively. The batch cultures in the bioreactor confirmed the best performance of DSM 8218. A two-stage fed-batch strategy-growth phase in aliphatic hydrocarbons, followed by the addition of WMO (one pulse of 5 g·L-1 or five pulses of 1 g·L-1 WMO), significantly increased biomass production and WMO assimilation by both strains. In experiments with five pulses, CBS 2075 and DSM 8218 strains reached high proteolytic activities (593-628 U·L-1) and accumulated high quantities of intracellular lipids (1.3-1.7 g·L-1). Yeast lipids, mainly composed of oleic and linoleic acids with an unsaturated/saturated fraction > 59%, meet the EU biodiesel standard EN 14214, making them suitable for biodiesel production.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596002/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0