Journal of Fungi最新文献_第3页

Isolation and Identification of Endophytic Bacterium B5 from Mentha haplocalyx Briq. and Its Biocontrol Mechanisms Against Alternaria alternata-Induced Tobacco Brown Spot. 薄荷内生细菌B5的分离与鉴定。烟草褐斑病的生物防治机制研究

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-06-12 DOI: 10.3390/jof11060446

Qunying Qin, Boyu Liu, Baige Ma, Xihong Wei, Yi Zhou, Zhengxiang Sun

{"title":"Isolation and Identification of Endophytic Bacterium B5 from Mentha haplocalyx Briq. and Its Biocontrol Mechanisms Against Alternaria alternata-Induced Tobacco Brown Spot.","authors":"Qunying Qin, Boyu Liu, Baige Ma, Xihong Wei, Yi Zhou, Zhengxiang Sun","doi":"10.3390/jof11060446","DOIUrl":"10.3390/jof11060446","url":null,"abstract":"The fungus Alternaria alternata, which causes tobacco brown spot disease, poses a serious threat to the tobacco industry. Beneficial microorganisms and their secondary metabolites have emerged as a promising green strategy for disease management. This study recovered 16 endophytic bacterial strains from Mentha haplocalyx Briq., a therapeutic herb. The study revealed that strain B5, with an inhibition rate of 82.76%, exhibited the highest antifungal activity against A. alternata. This strain exhibited broad-spectrum antifungal activity, with inhibition rates ranging from 66.34% to 87.23%. Phylogenetic analysis of 16S rDNA and gyrA gene sequences identified it as Bacillus velezensis (GenBank: PV168970 and PV173738). Further characterization revealed that strain B5 can secrete cell wall-degrading enzymes, produce IAA, and synthesize siderophores. The growth of mycelium in A. alternata was greatly reduced by both the ethyl acetate extract and the filtered liquid from the sterile fermentation, resulting in marked morphological abnormalities. Multiple antifungal active substances were identified through liquid LC-MS analysis. Greenhouse experiments demonstrated that the B5 fermentation broth effectively suppressed the occurrence of tobacco brown spot disease, achieving a relative control efficacy of 60.66%, comparable to that of 10% difenoconazole water dispersible granule (WDG). Additionally, strain B5 enhances plant disease resistance by activating the activities of key defense enzymes. B. velezensis B5 serves as a safe alternative to chemical fungicides and is highly effective at controlling tobacco brown spot disease.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tar Spot on Maize: Impact of Soil Types and Environmental Conditions on the Survival of Phyllachora maydis in the Subtropical Climate of Florida. 佛罗里达亚热带气候下玉米焦油斑：土壤类型和环境条件对毛叶蚜存活的影响

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-06-11 DOI: 10.3390/jof11060443

Vitor A S Moura, Larissa C Ferreira, Marcio F R Resende, Katia V Xavier

{"title":"Tar Spot on Maize: Impact of Soil Types and Environmental Conditions on the Survival of Phyllachora maydis in the Subtropical Climate of Florida.","authors":"Vitor A S Moura, Larissa C Ferreira, Marcio F R Resende, Katia V Xavier","doi":"10.3390/jof11060443","DOIUrl":"10.3390/jof11060443","url":null,"abstract":"Tar spot, caused by Phyllachora maydis, is an established maize disease in the Midwest of the United States but remains an emerging concern in Florida. While this pathogen can overwinter on plant residue, its survival in Florida's subtropical environment is not well understood. This study evaluated how environmental factors affect the germination of P. maydis ascospores and stroma integrity. Symptomatic maize leaves were incubated under four conditions: Histosol soil (muck), Krome soil (rocky), 4 °C, and 23 °C. Extensive leaf decomposition occurred in both soil types, with most plant material degraded after eight weeks, while the stroma maintained its structure. Despite this, ascospore germination declined across all conditions. After eight weeks, ascospores incubated at 4 °C retained 25% viability, while those at 23 °C had the lowest germination (0.7%). Ascospores from leaves buried in soil exhibited low viability (1-6%), with no significant differences between soil types (p=0.9944). Weather analysis revealed that increased temperature reduced germination rates, while higher humidity enhanced them. These findings suggest that P. maydis displays limited survivability under Florida-like conditions, with germination rates declining over time. Therefore, cultural practices such as tillage, already employed by corn producers in Florida, may be effective in reducing sources of P. maydis inoculum.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pilot Study Comparing the In Vitro Response of Circulating Monocytes to Aspergillus fumigatus Swollen Conidia in Patients with Chronic Graft-Versus-Host Disease and Healthy Volunteers. 比较慢性移植物抗宿主病患者和健康志愿者循环单核细胞对烟曲霉肿胀分生孢子体外反应的初步研究。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-06-11 DOI: 10.3390/jof11060444

Claire Kenny, Charles Oliver Morton, Eibhlin Conneally, Ann Atzberger, Anthony Davies, Hermann Einsele, Juergen Loeffler, Thomas R Rogers

{"title":"Pilot Study Comparing the In Vitro Response of Circulating Monocytes to Aspergillus fumigatus Swollen Conidia in Patients with Chronic Graft-Versus-Host Disease and Healthy Volunteers.","authors":"Claire Kenny, Charles Oliver Morton, Eibhlin Conneally, Ann Atzberger, Anthony Davies, Hermann Einsele, Juergen Loeffler, Thomas R Rogers","doi":"10.3390/jof11060444","DOIUrl":"10.3390/jof11060444","url":null,"abstract":"Invasive fungal disease (IFD) is a recognised and potentially life-threatening complication of chronic graft-versus-host disease (cGVHD) and its treatment. Invasive aspergillosis (IA), most often due to the species Aspergillus fumigatus, is the leading IFD in this setting. IA can occur during the early weeks following allogeneic haematopoietic stem cell transplantation (HSCT) coinciding with profound neutropenia, but increasingly, cases of IA occur after engraftment, coinciding with the occurrence of cGVHD. Immunomodulatory treatments of cGVHD can impair innate immune responses to inhaled Aspergillus conidia, increasing the risk of developing IA. Here, in a pilot study, we present an analysis of the phenotypic characteristics (phagocytic efficiency, fungal killing, and cytokine release) of circulating monocytes derived from patients with cGVHD compared to healthy volunteers. We found that there was no statistically significant difference in their ability to phagocytose A. fumigatus conidia, and while there was a trend in their reduced ability to kill conidia, this was not significant when compared to the ability of volunteers' monocytes to do so. Although we could not demonstrate in this small cohort of patients with cGVHD that monocytes may be a factor in the increased susceptibility to IA, further investigation of larger numbers of study subjects is warranted so that in vitro biomarkers may be developed for immune responses to Aspergillus in patients with cGVHD.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Candida glabrata Prosthetic Joint Infection Managed with Ibrexafungerp. 人工关节假丝酵母菌感染治疗。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-06-11 DOI: 10.3390/jof11060442

Ella Nadarevic, Jack McHugh, Paschalis Vergidis

引用次数: 0

Biocontrol and Mycotoxin Mitigation: An Endophytic Fungus from Maize Exhibiting Dual Antagonism Against Fusarium verticillioides and Fumonisin Reduction. 生物防治和缓解霉菌毒素：一种玉米内生真菌对黄萎病镰刀菌具有双重拮抗作用和还原伏马菌素。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-06-11 DOI: 10.3390/jof11060441

Qianhui Li, Dongbeng Zhang, Dongyan Ye, Shuo Zhang, Qiurui Ma, Helong Bai, Fanlei Meng

{"title":"Biocontrol and Mycotoxin Mitigation: An Endophytic Fungus from Maize Exhibiting Dual Antagonism Against Fusarium verticillioides and Fumonisin Reduction.","authors":"Qianhui Li, Dongbeng Zhang, Dongyan Ye, Shuo Zhang, Qiurui Ma, Helong Bai, Fanlei Meng","doi":"10.3390/jof11060441","DOIUrl":"10.3390/jof11060441","url":null,"abstract":"Fusarium verticillioides is one of the pathogenic fungi causing maize ear rot, and its secreted fumonisins accumulated in plants pose significant threats to human health. To reduce the incidence of maize ear rot and fumonisin contamination, this study isolated numerous endophytic fungi from maize plants. Through inhibition zone and dual culture assays, an endophytic fungal strain, FJ284, demonstrating notable antifungal activity against F. verticillioides was screened. 18S rDNA gene sequencing was employed for fungal identification, and the sequences were deposited in NCBI GenBank. FJ284 was identified as Penicillium oxalicum. The ethyl acetate extract of P. oxalicum was analyzed using gas chromatography-mass spectrometry (GC-MS), revealing 52 compounds, including several secondary metabolites with documented anticancer, antimicrobial, and antioxidant activities. Furthermore, a spectroscopic method was developed to assess the inhibitory effect of strain FJ284 against F. verticillioides, showing maximum inhibition at 48 h. Finally, Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS) analyses confirmed that FJ284 significantly inhibited three fumonisins (suppression rates > 50%), with efficacy ranked as fumonisin B3 (FB3) > fumonisin B2 (FB2) > fumonisin B1 (FB1).","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response to Oxidative Stress in Sporothrix schenckii. 申氏孢子丝菌对氧化应激的响应。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-06-10 DOI: 10.3390/jof11060440

Estela Ruiz-Baca, Pablo Jaciel Adame-Soto, Carlos Antonio Alba-Fierro, Ana Lilia Martínez-Rocha, Armando Pérez-Torres, Angélica López-Rodríguez, Yolanda Romo-Lozano

{"title":"Response to Oxidative Stress in Sporothrix schenckii.","authors":"Estela Ruiz-Baca, Pablo Jaciel Adame-Soto, Carlos Antonio Alba-Fierro, Ana Lilia Martínez-Rocha, Armando Pérez-Torres, Angélica López-Rodríguez, Yolanda Romo-Lozano","doi":"10.3390/jof11060440","DOIUrl":"10.3390/jof11060440","url":null,"abstract":"Oxidative stress is key in immune defense against fungal infections, such as those caused by Sporothrix schenckii, the dimorphic fungus responsible for sporotrichosis. Phagocytic cells utilize oxidative stress as a crucial mechanism to control pathogen spread. During S. schenckii infection, phagocytic cells recognize pathogen-associated molecular patterns (PAMPs) on their surface through conserved transmembrane or soluble receptors, known as pattern recognition receptors (PRRs). This recognition triggers a cascade of immune responses, including the generation reactive oxygen species (ROS) essential for pathogen elimination. However, S. schenckii has developed sophisticated mechanisms to evade and counteract this response, contributing to its persistence in the host. These mechanisms include the production of antioxidant enzymes, alterations to its cell wall (CW), and the production of melanin, which helps neutralize oxidative stress. In addition, S. schenckii modulates the production of other proteins, such as moonlighting proteins, suggested to have roles in immune evasion and stress response, helping its survival in the host. These strategies, along with the modulation of gene expression, allow the fungus to survive and persist inside the immune system's hostile environment, facilitating the progression of the infection. Understanding these interactions between phagocytic cells and S. schenckii is key to developing more effective therapeutic strategies to combat sporotrichosis.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a High-Performance Trichoderma Mutant for Enhanced Cellulase Production Through UV-Induced Random Mutagenesis. 通过紫外诱导随机诱变提高纤维素酶产量的高效木霉突变体的开发。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-06-09 DOI: 10.3390/jof11060439

Seungjun Kim, Iksu Ha, Yun-Yeong Lee, Junseo Lee, Jeonghee Yun

{"title":"Development of a High-Performance Trichoderma Mutant for Enhanced Cellulase Production Through UV-Induced Random Mutagenesis.","authors":"Seungjun Kim, Iksu Ha, Yun-Yeong Lee, Junseo Lee, Jeonghee Yun","doi":"10.3390/jof11060439","DOIUrl":"10.3390/jof11060439","url":null,"abstract":"Ultraviolet (UV)-induced mutagenesis is a cost-effective and straightforward technique for introducing random genetic variations without the use of chemical reagents or genetic engineering. It is commonly employed to enhance enzyme activity in industrial trains. In this study, Trichoderma sp. was exposed to UV radiation at varying distances (4, 9, and 13 cm) and durations (2, 4, 6, and 8 min) to induce mutations. The activities of endoglucanase (EG), β-glucosidase (BGL), and cellobiohydrolase (CBH) were assessed following treatment. The 4 cm exposure distance yielded the highest enhancement, with EG, BGL, and CBH activities increasing 1.5-, 1.3-, and 0.9-fold, respectively. When the distance was fixed at 4 cm, the optimal exposure time was identified as 4 min, yielding further enhancements of 1.9-, 1.6-, and 1.4-fold, respectively. The resulting mutant, designated Mut-4, was scaled up in a 10-L bioreactor to assess its industrial applicability. Mut-4 retained its enhanced performance, achieving 1.9-, 2.0-, and 1.4-fold enhancements in EG, BGL, and CBH activities, respectively, compared with the original strain. These findings indicate that combining UV-induced mutagenesis with basic screening is an effective strategy for enhancing cellulolytic enzyme production, representing a promising approach for lignocellulosic biomass conversion.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inbreeding and Genetic Differentiation Among Geographic Populations of Lactarius hatsudake in Southwest China. 西南地区鹿茸地理居群的近交与遗传分化。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-06-08 DOI: 10.3390/jof11060438

Kuan Zhao, Mingwei Mao, Xianghua Wang, Jianping Xu

{"title":"Inbreeding and Genetic Differentiation Among Geographic Populations of Lactarius hatsudake in Southwest China.","authors":"Kuan Zhao, Mingwei Mao, Xianghua Wang, Jianping Xu","doi":"10.3390/jof11060438","DOIUrl":"10.3390/jof11060438","url":null,"abstract":"Lactarius hatsudake is an economically important wild edible fungus in sub-tropical and temperate Asia. At present, little is known about its genetic diversity, mode of reproduction, and population structure in natural environments. In this study, we collected 102 specimens from eight geographic locations across three provinces in southwest China. Five simple sequence repeat markers that demonstrated high polymorphism were developed and used to analyze the patterns of genetic variations within and among the eight geographic populations. Analyses of molecular variance revealed that 60% of the observed genetic variation occurred among populations, with the remaining 40% attributable to within populations, while inter-provincial variation was nonsignificant. Combined analyses based on discriminant analysis of principal components, STRUCTURE, and the unweighted pair group method with arithmetic mean identified two distinct genetic subpopulations with each genetic subpopulation showing a wide geographical distribution, a result consistent with ancient divergence and recent gene flow within this species in southwest China. Interestingly, excess homozygosity was observed at most loci in almost all of the eight geographic populations, consistent with inbreeding being common for this species in nature. Together, our results revealed the genetic diversity, mode of reproduction, and geographic structuring of this important wild mushroom in southwest China.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proposed Diagnostic Criteria for Invasive Pulmonary Aspergillosis in Patients with Autoimmune Inflammatory Rheumatic Diseases: A Proof-of-Concept Study. 自身免疫性风湿性疾病患者侵袭性肺曲霉病的拟议诊断标准：一项概念验证研究

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-06-07 DOI: 10.3390/jof11060437

Takashi Kurita, Koh Okamoto, Noritaka Sekiya, Ryoichi Hanazawa, Akio Yamamoto, Tadashi Hosoya, Akihiro Hirakawa, Shinsuke Yasuda, Yoshiaki Gu

{"title":"Proposed Diagnostic Criteria for Invasive Pulmonary Aspergillosis in Patients with Autoimmune Inflammatory Rheumatic Diseases: A Proof-of-Concept Study.","authors":"Takashi Kurita, Koh Okamoto, Noritaka Sekiya, Ryoichi Hanazawa, Akio Yamamoto, Tadashi Hosoya, Akihiro Hirakawa, Shinsuke Yasuda, Yoshiaki Gu","doi":"10.3390/jof11060437","DOIUrl":"10.3390/jof11060437","url":null,"abstract":"The EORTC/MSGERC definition lacks sufficient sensitivity for diagnosing invasive pulmonary aspergillosis (IPA) in patients with autoimmune inflammatory rheumatic diseases (AIIRDs). We hypothesized that the partial fulfillment of the EORTC/MSGERC definition can improve its diagnostic sensitivity. This retrospective observational study included patients with AIIRDs on immunosuppressive therapy who underwent serum galactomannan antigen testing for suspected IPA. Patients who fulfilled the clinical features or mycological evidence as per the EORTC/MSGERC definition were considered as having \"potential IPA.\" We compared the clinical characteristics of 364 patients who were categorized into 3 groups-potential IPA (n = 29), proven/probable IPA (n = 24), and non-IPA (n = 311; not meeting any definition). The potential and proven/probable IPA groups had significantly lower survival rates than the non-IPA group (p < 0.001). The potential IPA (adjusted hazard ratio [aHR], 2.0; 95% confidence interval [CI], 1.1-3.8) and proven/probable IPA (aHR, 2.6; 95% CI, 1.4-4.9) were independent risk factors for mortality. Compared with the EORTC/MSGERC definition, our proposed criteria improved sensitivity based on the diagnosis at the end of observation (50.0%, 100.0%, respectively). The characteristics and mortality rates of patients were similar between the potential and proven/probable IPA groups. Using these criteria for clinical diagnosis may provide high sensitivity.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in Diagnosing Talaromycosis: Exploring Novel Strategies and Emerging Technologies. 塔拉芳香菌病的诊断进展：探索新策略和新兴技术。

IF 4.2 2区生物学

Journal of Fungi Pub Date : 2025-06-06 DOI: 10.3390/jof11060434

Yihui Wang, Xiaoyue Wang, Liyan Xi, Sha Lu

{"title":"Advancements in Diagnosing Talaromycosis: Exploring Novel Strategies and Emerging Technologies.","authors":"Yihui Wang, Xiaoyue Wang, Liyan Xi, Sha Lu","doi":"10.3390/jof11060434","DOIUrl":"10.3390/jof11060434","url":null,"abstract":"Talaromycosis (TM) is an invasive fungal infection caused by Talaromyces marneffei (T. marneffei). It has high morbidity and mortality rates, particularly among immunocompromised people. Globally, approximately 17,300 cases and 4900 deaths are reported annually. TM often has vague clinical signs with limited current tests, leading to misdiagnosis, incorrect treatments, or the long-term use of expensive antifungal drugs, which raises healthcare costs and patient risks. Although accurate diagnosis is key for starting the right antifungal therapy and improving outcomes, there are not enough reliable and fast tests. Recent progress with monoclonal antibodies (mAbs) that have high specificity for antigens may boost diagnostic accuracy and cut misdiagnosis rates. This review explores current ways to diagnose TM, including culture, histopathology, and molecular methods such as polymerase chain reaction (PCR) and antigen detection. We also discuss the merits and weaknesses of each method and highlight how mAbs may help diagnose TM. We searched PubMed, Web of Science, and Google Scholar for English-language papers (1990-1 January 2025) using \"Talaromycosis\" OR \"Talaromyces marneffei\" plus diagnostic terms ('diagnosis', 'molecular diagnostics', 'monoclonal antibody', 'lateral flow', 'antigen detection', and 'fungal diagnosis'). After deduplication and relevance screening, studies with original data or substantive discussion on T. marneffei diagnostics or mAb development were retained to inform this narrative review.","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 6","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0