Rafaelly Camargo, Jonatas Silva de Oliveira, Amanda Costa Ferro, Beatriz Ribeiro Ribas, Alan Augusto Valério Alves, Janaina Habib Jorge
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引用次数: 0
Abstract
Studies assessing the long-term effects of brushing with cleaning and disinfecting agents on surface roughness and biofilm accumulation on the three-dimensionally (3D) printed resins remain scarce. This study aimed to evaluate the effects of different solutions and simulated brushing times on the surface roughness and biofilm formation on heat-cured and 3D-printed denture base resins. Discs samples (10 mm × 1.2 mm) were prepared and randomly assigned to brushing treatments using the following solutions: distilled water, disinfectant liquid soap and dentifrice. The specimens (n = 9) were subjected to 10,000, 20,000 and 50,000 cycles to simulate 1, 2 and 5 years of brushing, respectively. The control group consisted of unbrushed samples. Surface roughness (Ra) was measured. Candida albicans biofilm formation was evaluated by counting colony forming units, cellular metabolic activity, and qualitative and quantitative analysis through confocal fluorescence microscopy. A significant increase in roughness was observed in both resins after two years of simulated brushing, mainly with dentifrice. After two years of brushing, an increase in the number of cells and metabolism of C. albicans was observed, in agreement with the fluorescence and biofilm thickness results. Brushing with dentifrice increased the roughness of heat-cured and 3D-printed resins and potentially increased C. albicans biofilm formation.
评估清洁和消毒剂对三维(3D)打印树脂表面粗糙度和生物膜积累的长期影响的研究仍然很少。本研究旨在评估不同溶液和模拟刷牙次数对热固化和3d打印义齿基托树脂表面粗糙度和生物膜形成的影响。制备10 mm × 1.2 mm的盘状样品,并随机分配使用以下溶液进行刷牙处理:蒸馏水、消毒液体皂和牙膏。9个样本分别进行了10,000、20,000和50,000次循环,以模拟1年、2年和5年的刷牙。对照组由未刷毛的样品组成。测量表面粗糙度(Ra)。通过计数菌落形成单位、细胞代谢活性和共聚焦荧光显微镜的定性和定量分析来评估白色念珠菌生物膜的形成。经过两年的模拟刷牙(主要是用牙膏),两种树脂的粗糙度都显著增加。经过两年的刷牙,观察到白色念珠菌的细胞数量和代谢增加,与荧光和生物膜厚度的结果一致。用牙膏刷牙增加了热固化和3d打印树脂的粗糙度,并可能增加白色念珠菌生物膜的形成。
期刊介绍:
Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.