Integrative Transcriptome and Metabolome Analysis Reveals Candidate Genes Related to Terpenoid Synthesis in Amylostereum areolatum (Russulales: Amylostereaceae).

IF 4.2 2区生物学 Q2 MICROBIOLOGY

Journal of Fungi Pub Date : 2025-05-16 DOI:10.3390/jof11050383

Lixia Wang, Ningning Fu, Ming Wang, Zhongyi Zhan, Youqing Luo, Jianrong Wu, Lili Ren

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引用次数: 0

Abstract

Amylostereum areolatum (Chaillet ex Fr.) Boidin (Russulales: Amylostereaceae) is a symbiotic fungus of Sirex noctilio Fabricius that has ecological significance. Terpenoids are key mediators in fungal-insect interactions, yet the biosynthetic mechanisms of terpenoids in this species remain unclear. Under nutritional conditions that mimic natural growth, A. areolatum was sampled during the lag phase (day 7), exponential phase (day 14), and stationary phase (day 21). Metabolome (solid-phase microextraction (SPME) combined with gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS)) and transcriptome (Illumina NovaSeq) profiles were integrated to investigate terpenoid-gene correlations. This analysis identified 103 terpenoids in A. areolatum, substantially expanding the known repertoire of terpenoid compounds in this species. Total terpenoid abundance progressively increased across three developmental stages, with triterpenoids and sesquiterpenoids demonstrating the highest diversity and abundance levels. Transcriptomic profiling (61.66 Gb clean data) revealed 26 terpenoid biosynthesis-associated genes, establishing a comprehensive transcriptional framework for fungal terpenoid metabolism. Among 11 differentially expressed genes (DEGs) (|log2Fold Change| ≥ 1, adjusted p < 0.05), HMGS1, HMGR2, and AaTPS1-3 emerged as key regulators potentially governing terpenoid biosynthesis. These findings provide foundational insights into the molecular mechanisms underlying terpenoid production in A. areolatum and related basidiomycetes.

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综合转录组和代谢组分析揭示了直链淀粉萜类合成相关的候选基因。

Amylostereum areolatum (Chaillet ex Fr.) Boidin （Russulales: Amylostereaceae）是一种具有生态意义的夜蛾（Sirex noctilio Fabricius）共生真菌。萜类化合物是真菌与昆虫相互作用的关键介质，但萜类化合物在该物种中的生物合成机制尚不清楚。在模拟自然生长的营养条件下，分别在滞后期（第7天）、指数期（第14天）和稳定期（第21天）取样。结合代谢组（固相微萃取（SPME）、气相色谱-质谱（GC-MS）和液相色谱-质谱（LC-MS））和转录组（Illumina NovaSeq）谱来研究萜类化合物与基因的相关性。该分析鉴定出了103种萜类化合物，大大扩展了该物种中已知的萜类化合物库。总萜类丰度在三个发育阶段逐渐增加，其中三萜类和倍半萜类的多样性和丰度最高。转录组学分析（61.66 Gb干净数据）揭示了26个萜类生物合成相关基因，建立了真菌萜类代谢的综合转录框架。在11个差异表达基因（DEGs）（|log2Fold Change|≥1，调整后p < 0.05）中，HMGS1、HMGR2和AaTPS1-3被认为是控制萜类生物合成的关键调控因子。这些发现提供了基础见解的分子机制下萜类化合物的生产在a.a areolatum和相关担子菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Fungi Medicine-Microbiology (medical)

CiteScore

6.70

自引率

14.90%

发文量

1151

审稿时长

11 weeks

期刊介绍： Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.