Journal of Fungi最新文献

{"title":"Four New Species of <i>Deconica</i> (Strophariaceae, Agaricales) from Subtropical Regions of China.","authors":"Jun-Qing Yan, Sheng-Nan Wang, Ya-Ping Hu, Cheng-Feng Nie, Bin-Rong Ke, Zhi-Heng Zeng, Hui Zeng","doi":"10.3390/jof10110745","DOIUrl":"10.3390/jof10110745","url":null,"abstract":"<p><p><i>Deconica</i> is a relatively small genus, with only 90 names recorded in previous research. In this study, four new species of <i>Deconica</i> have been identified based on morphological and phylogenetic evidence from subtropical regions of China. This represents the first discovery of new species of <i>Deconica</i> in China. Morphologically, <i>D</i>. <i>austrosinensis</i> is characterized by medium-sized spores that are elliptical to elongated-ellipsoid in face view, and fusiform to sublageniform and slightly thick-walled pleurocystidia; <i>D</i>. <i>furfuracea</i> is identified by a well-developed and evanescent veil, medium-sized spores that are rhomboid to mitriform in face view, and fusiform to subclavate pleurocystidia that are rare and subacute at apex; <i>D</i>. <i>fuscobrunnea</i> is recognized by dark brown pileus, medium-sized spores that are rhomboid to mitriform in face view, an ixocutis pileipellis, lageniform cheilocystidia with a long neck and lacks pleurocystidia; <i>D</i>. <i>ovispora</i> is distinguished from other <i>Deconica</i> species by medium-sized spores that are ovoid in face view, an ixocutis pileipellis, lageniform cheilocystidia with a long to short neck, and lacks pleurocystidia. Their distinct taxonomic status is confirmed by the positions of the four new species in ITS + LSU phylogenetic trees. Detailed descriptions and morphological photographs of four new species are presented.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Key Enzymes of Carbon Metabolism and the Glutathione Antioxidant System Protect <i>Yarrowia lipolytica</i> Yeast Against pH-Induced Stress.","authors":"Tatyana I Rakhmanova, Natalia N Gessler, Elena P Isakova, Olga I Klein, Yulia I Deryabina, Tatyana N Popova","doi":"10.3390/jof10110747","DOIUrl":"10.3390/jof10110747","url":null,"abstract":"<p><p>In this study, we first thoroughly assayed the response of the key enzymes of energy metabolism and the antioxidant system in <i>Yarrowia lipolytica</i> yeast at extreme pH. The activity of the tricarboxylic acid cycle enzymes, namely NAD-dependent isocitrate dehydrogenase, aconitate hydratase, NAD-dependent malate dehydrogenase, and fumarate hydratase, NADPH-producing enzymes of glucose-6-P dehydrogenase and NADP-dependent isocitrate dehydrogenase, and the enzymes of the glutathione system was assessed. All the enzymes that were tested showed a significant induction contrary to some decrease in the aconitate hydratase activity with acidic and alkaline stress. It is probable that a change in the enzyme activity in the mitochondria matrix is involved in the regulation of the cellular metabolism of <i>Y. lipolytica</i>, which allows the species to prosper at an extreme ambient pH. It distinguishes it from any other type of ascomycete. A close relationship between the induction of the Krebs cycle enzymes and the key enzymes of the glutathione system accompanied by an increased level of reduced glutathione was shown. The assumption that the increased activity of the Krebs cycle dehydrogenases and promotion of the pentose phosphate pathway at pH stress launches a set of events determining the adaptive response of <i>Y. lipolytica</i> yeast.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of <i>Candida</i> Species Is Associated with Comorbidities, Prolonged Mechanical Ventilation, and Treatment Outcomes in Surgical ICU Patients, a Cross-Sectional Study.","authors":"Josipa Glavaš Tahtler, Ana Cicvarić, Despoina Koulenti, Marios Karvouniaris, Maja Bogdan, Kristina Kralik, Irena Krajina Kmoniček, Marina Grbić Mlinarević, Slavica Kvolik","doi":"10.3390/jof10110743","DOIUrl":"10.3390/jof10110743","url":null,"abstract":"<p><p>The isolation of <i>Candida</i> may be related to comorbidity, prolonged mechanical ventilation, and survival during intensive care unit (ICU) stay, especially with non-albicans <i>Candida</i> (NAC). To examine the frequency of <i>Candida</i> isolation, associated comorbidities and outcomes in the surgical ICU in Osijek University Hospital, Croatia, the data from the electronic database from May 2016 to 30 June 2023 were analyzed. In a cross-sectional study examining 15,790 microbiological samples, different strains of <i>Candida</i> were observed in 581 samples from 236 patients. The control group (N = 261) was 130 consecutive patients from March to May 2019 and 131 in the same months in 2020 (pre- and post-COVID-19). Comorbidities, duration of mechanical ventilation, and survival were compared. Patients with isolated <i>Candida</i> were more often non-elective and had significantly more heart, kidney, and liver diseases and sepsis than the control group (<i>p</i> < 0.001). The duration of mechanical ventilation was 9.2 [2.2-9.24], 96 [24-146], 160 [19.5-343], and 224 [73.5-510] hours in the controls, in patients with <i>Candida albicans</i>, in patients with NAC, and in patients with ≥2 <i>Candida</i> species isolated, respectively. The mortality was significantly higher (42%) in patients with isolated <i>Candida</i> than in the control group (19%, <i>p</i> < 0.001). In a multivariate analysis adjusted for patients' age, the Simplified Acute Physiology Score II, days of ICU, and type of admission, only sepsis on admission was an independent predictor of mortality (odds ratio = 2.27).</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Characterization and Pathogenicity of <i>Colletotrichum falcatum</i> Causing Red Rot on Sugarcane in Southern Florida.","authors":"Fernanda Rodrigues Silva, Mário Lúcio V de Resende, Larissa Carvalho Ferreira, Olamide Adesina, Katia V Xavier","doi":"10.3390/jof10110742","DOIUrl":"10.3390/jof10110742","url":null,"abstract":"<p><p>Red rot disease reduces sugarcane yield and impacts the sugar quality, posing an important threat to the sugarcane industry in Florida. Although <i>Colletotrichum falcatum</i>, the causal agent of red rot in Florida, was first reported in 1984 based on morphology, molecular and pathological data have remained limited, highlighting the critical need for comprehensive characterization. Thirteen isolates were obtained from three local sugarcane varieties in Belle Glade, Florida. Phylogenetic analyses of five genetic markers (ITS, <i>ACT</i>, <i>TUB2</i>, <i>GAPDH</i>, and <i>CHS-1</i>) confirmed all the strains as <i>C. falcatum</i>. In addition, the study documented the disease progression at the cellular level and assessed the pathogenicity of representative strains using the leaf sheath and whole-seedling inoculation methods. The varieties CP96-1252 and CP89-2143 showed greater host resistance. These findings represent the first report of <i>C. falcatum</i> causing red rot in southern Florida, offer valuable insights for/into red rot management, and provide a basis for future breeding programs to enhance sugarcane resistance to red rot disease.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of Catalase Expression and Activity by <i>Dh</i>Hog1 in the Halotolerant Yeast <i>Debaryomyces hansenii</i> Under Saline and Oxidative Conditions.","authors":"Ileana de la Fuente-Colmenares, James González, Norma Silvia Sánchez, Daniel Ochoa-Gutiérrez, Viviana Escobar-Sánchez, Claudia Segal-Kischinevzky","doi":"10.3390/jof10110740","DOIUrl":"10.3390/jof10110740","url":null,"abstract":"<p><p>Efficient transcriptional regulation of the stress response is critical for microorganism survival. In yeast, stress-related gene expression, particularly for antioxidant enzymes like catalases, mitigates reactive oxygen species such as hydrogen peroxide (H₂O₂), preventing cell damage. The halotolerant yeast <i>Debaryomyces hansenii</i> shows oxidative stress tolerance, largely due to high catalase activity from <i>DhCTA</i> and <i>DhCTT</i> genes. This study evaluates <i>D. hansenii'</i>s response to oxidative stress caused by H₂O₂ under saline conditions, focusing on cell viability, gene expression, and catalase activity. Chromatin organization in the promoter of <i>DhCTA</i> and <i>DhCTT</i> was analyzed, revealing low nucleosome occupancy in promoter regions, correlating with active gene expression. Stress-related motifs for transcription factors like Msn2/4 and Sko1 were found, suggesting regulation by the <i>DhHog1</i> MAP kinase. Analysis of a <i>Dhhog1</i>Δ mutant showed <i>Dh</i>Hog1's role in <i>DhCTA</i> expression under H₂O₂ or NaCl conditions. These findings highlight <i>Dh</i>Hog1's critical role in regulating the stress response in <i>D. hansenii</i>, offering insights for enhancing stress tolerance in halotolerant yeasts, particularly for industrial applications in saline wastewater management.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-Free Optical Transmission Tomography for Direct Mycological Examination and Monitoring of Intracellular Dynamics.","authors":"Eliott Teston, Marc Sautour, Léa Boulnois, Nicolas Augey, Abdellah Dighab, Christophe Guillet, Dea Garcia-Hermoso, Fanny Lanternier, Marie-Elisabeth Bougnoux, Frédéric Dalle, Louise Basmaciyan, Mathieu Blot, Pierre-Emmanuel Charles, Jean-Pierre Quenot, Bianca Podac, Catherine Neuwirth, Claude Boccara, Martine Boccara, Olivier Thouvenin, Thomas Maldiney","doi":"10.3390/jof10110741","DOIUrl":"10.3390/jof10110741","url":null,"abstract":"<p><p>Live-cell imaging generally requires pretreatment with fluorophores to either monitor cellular functions or the dynamics of intracellular processes and structures. We have recently introduced full-field optical coherence tomography for the label-free live-cell imaging of fungi with potential clinical applications for the diagnosis of invasive fungal mold infections. While both the spatial resolution and technical set up of this technology are more likely designed for the histopathological analysis of tissue biopsies, there is to our knowledge no previous work reporting the use of a light interference-based optical technique for direct mycological examination and monitoring of intracellular processes. We describe the first application of dynamic full-field optical transmission tomography (D-FF-OTT) to achieve both high-resolution and live-cell imaging of fungi. First, D-FF-OTT allowed for the precise examination and identification of several elementary structures within a selection of fungal species commonly known to be responsible for invasive fungal infections such as <i>Candida albicans</i>, <i>Aspergillus fumigatus</i>, or <i>Rhizopus arrhizus</i>. Furthermore, D-FF-OTT revealed the intracellular trafficking of organelles and vesicles related to metabolic processes of living fungi, thus opening new perspectives in fast fungal infection diagnostics.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chinese Populations of <i>Magnaporthe oryzae</i> Serving as a Source of Human-Mediated Gene Flow to Asian Countries: A Population Genomic Analysis.","authors":"Guohua Duan, Yuchan Liu, Cheng Zheng, Kaihui Yu, Jiahui Xie, Baohua Wang, Huakun Zheng, Wei Tang, Jiandong Bao, Zonghua Wang, Meilian Chen","doi":"10.3390/jof10110739","DOIUrl":"10.3390/jof10110739","url":null,"abstract":"<p><p><i>Magnaporthe oryzae</i>, a filamentous heterothallic ascomycete fungus that serves as the causative agent of rice blast disease, is globally distributed in rice-growing regions. Populations shaped by environmental factors and human intervention play important roles in the formation of genetic structure. In this study, population structures and spatiotemporal dynamics were investigated based on large-scale whole genomic sequences of rice-infecting <i>M. oryzae</i> around the world. By analyzing these genetic structures, we identified divergent clades that crossed geographic boundaries. While we observed associations between the isolates and their geographic origins, we also found that there were frequent migration events occurring across Asia in main rice cultivation regions. Within Asia, China was the migration origin, facilitating gene flows to Japan and South Korea. Since the 1970s, the genetic diversity of <i>M. oryzae</i> populations in China has also shown a steadily increasing trend, continuing through to the 2020s. Additionally, our analysis of the evolutionary history of Asian <i>M. oryzae</i> populations provided insights into the population expansion that has taken place in recent decades. Overall, our findings indicate that human-mediated gene flows played a pivotal role in shaping the genetic structure of <i>M. oryzae</i>.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Harvest Delay and Barley Variety on Grain Nutritional Composition and Mycotoxin Contamination.","authors":"Eimantas Venslovas, Yuliia Kochiieru, Sigita Janavičienė, Lauksmė Merkevičiūtė-Venslovė, Mohammad Almogdad, Vadims Bartkevics, Zane Bērziņa, Romans Pavlenko","doi":"10.3390/jof10110738","DOIUrl":"10.3390/jof10110738","url":null,"abstract":"<p><p>This study investigated the effects of delayed harvesting, varying meteorological conditions, and barley variety on <i>Fusarium</i> spp. infection rates, nutritional composition, and mycotoxin contamination in barley grains. Field experiments were conducted from 2020 to 2022 and involved two barley varieties: 'Laureate' for malting and 'Luokė' for feed. The results indicated that the dominant <i>Fusarium</i> species isolated were <i>F. avenaceum</i>, <i>F. culmorum</i>, <i>F. poae</i>, <i>F. sporotrichioides</i>, <i>F. tricinctum</i>, and <i>F. equiseti</i>. These tended to increase in number with delayed harvest times and were more prevalent during harvest periods of higher precipitation (<i>p</i> < 0.05). Malting barley had higher starch and lower protein content compared to feed barley (<i>p</i> < 0.05). Delayed harvesting generally increased dry matter, crude fat, and crude ash contents while decreasing crude protein, zinc, and iron contents (<i>p</i> < 0.05). Mycotoxin analysis revealed significant differences under specific weather conditions. HT-2 toxin levels were higher under slightly warmer and wetter conditions during flowering, with harvest conditions similar to the long-term average. Zearalenone levels increased with dry, warm growing seasons followed by rainy harvests. Nivalenol and enniatin levels increased with rainy growing seasons and dry, warm harvests. Deoxynivalenol concentrations did not reach the limit of quantification throughout the study. No consistent trend was observed for higher contamination in any specific barley variety (<i>p</i> > 0.05). The strongest correlations between mycotoxins and nutritional value indicators were observed with less-studied mycotoxins, such as nivalenol and enniatins, which exhibited negative correlations with crude protein (<i>p</i> < 0.01), crude fat (<i>p</i> < 0.05), and zinc (<i>p</i> < 0.01), and positive correlations with crude ash (<i>p</i> < 0.05) and phosphorus (<i>p</i> < 0.01).</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity and Anti-Infectious Components of Cultivable Rhizosphere Fungi Derived from Three Species of <i>Astragalus</i> Plants in Northwestern Yunnan, China.","authors":"Guo-Jun Zhou, Wei-Jia Xiong, Wei Xu, Zheng-Rong Dou, Bo-Chao Liu, Xue-Li Li, Hao Du, Hai-Feng Li, Yong-Zeng Zhang, Bei Jiang, Kai-Ling Wang","doi":"10.3390/jof10110736","DOIUrl":"10.3390/jof10110736","url":null,"abstract":"<p><p><i>Astragalus</i>, a group of legume plants, has a pronounced rhizosphere effect. Many species of <i>Astragalus</i> with limited resource reserves are distributed in the high-altitude area of northern Yunnan, China. Although some of these plants have high medicinal value, the recognition of them is still at a low level. The aim of this research is to explore the species diversity of cultivable rhizofungi derived from <i>Astragalus acaulis</i>, <i>A. forrestii</i> and <i>A. ernestii</i> growing in a special high-cold environment of northwest Yunnan and discover anti-infective components from these fungi. A total of 93 fungal strains belonging to 38 species in 18 genera were isolated and identified. Antibacterial and antimalarial screening yielded 10 target strains. Among them, the ethyl acetate crude extract of the fermented substrate of the rhizofungus <i>Aspergillus calidoustus</i> AA12 derived from the plant <i>A. acaulis</i> showed broad-spectrum antibacterial activity and the best antimalarial activity. Further chemical investigation led to the first discovery of seven compounds from the species <i>A. calidoustus</i>, including sesterterpine 6-epi-ophiobolin G; three sesquiterpenes, penicisochroman A, pergillin and 7-methyl-2-(1-methylethylethlidene)-furo [3,2-H]isoquinoline-3-one; and three polyketides, trypacidin, 1,2-seco-trypacidin and questin. Among them, the compound 6-epi-ophiobolin G exhibited moderate to strong antibacterial activity against six Gram-positive pathogens with the minimum inhibitory concentration (MIC) ranging from 25 to 6.25 μg/mL and a prominent inhibitory effect on the biofilm of <i>Streptococcus agalactiae</i> at an MIC value of 3.125 μg/mL. This compound also displayed potent antimalarial activity against <i>Plasmodium falciparum</i> strains 3D7 and chloroquine-resistant Dd2 at the half-maximal inhibitory concentration (IC₅₀) values of 3.319 and 4.340 µmol/L at 72 h, respectively. This study contributed to our understanding of the cultivable rhizofungi from characteristic <i>Astragalus</i> plants in special high-cold environments and further increased the library of fungi available for natural anti-infectious product screening.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New Polyketide Congeners with Antibacterial Activities from an Endophytic Fungus <i>Stemphylium globuliferum</i> 17035 (China General Microbiological Culture Collection Center No. 40666).","authors":"Yingying Li, Guoliang Zhu, Jing Wang, Junjie Yu, Ke Ye, Cuiping Xing, Biao Ren, Bin Zhu, Simin Chen, Lijun Lai, Yue Li, Tom Hsiang, Lixin Zhang, Xueting Liu, Jingyu Zhang","doi":"10.3390/jof10110737","DOIUrl":"10.3390/jof10110737","url":null,"abstract":"<p><p>Four new polyketides, heterocornol Y (<b>1</b>), stemphyindan (<b>2</b>), pestalospirane C (<b>3</b>), and stemphyspyrane (<b>4</b>), along with five known ones (<b>5</b>-<b>9</b>) were isolated from the endophytic fungus <i>Stemphylium globuliferum</i> 17035 (SG17035) based on the One Strain Many Compounds (OSMAC) strategy allied with an LC-MS approach. These structures were elucidated through extensive spectroscopic analyses, single-crystal X-ray diffraction, and ¹³C NMR-DP4 analysis. Pestalospirane C (<b>3</b>) and stemphyspyrane (<b>4</b>) featured unprecedented spiroketal skeletons. In addition, the putative biosynthetic logic for compounds <b>1</b>-<b>4</b> was proposed. Antibacterial and cytotoxic activities of compounds <b>1</b>-<b>9</b> were evaluated. Stemphyspyrane (<b>4</b>) displayed promising antibacterial activity against different pathogens, especially against <i>Staphylococcus aureus, Porphyromonas gingivalis</i>, and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) with MIC values of 3.125 μM, 6.25 μM, and 12.5 μM, respectively. It is promising as an antibacterial agent for further optimization.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"10 11","pages":""},"PeriodicalIF":4.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}