Journal of Fungi最新文献

{"title":"The PKS-NRPS Gene <i>BBA_09856</i> Deletion Mutant of <i>Beauveria bassiana</i> Enhanced Its Virulence Against <i>Ostrinia furnacalis</i> Larvae and Strengthened the Host Plant's Resistance to <i>Botrytis cinerea</i> as an Endotype.","authors":"Yanan Wang, Xiaowei Zou, Xiaomin Zhu, Ji Qi, Jianfeng Liu, Zhengkun Zhang","doi":"10.3390/jof11030197","DOIUrl":"10.3390/jof11030197","url":null,"abstract":"<p><p>Nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) play crucial roles in the development and pathogenicity of the entomopathogenic fungus <i>Beauveria bassiana</i>. However, they are among the few biosynthetic gene clusters with unknown functions in <i>B. bassiana</i>. To investigate the role of the hybrid PKS-NRPS synthetase gene <i>BBA_09856</i> in <i>B. bassiana</i>, we constructed a mutant strain, ∆<i>BBA09856</i>-WT, by deleting the <i>BBA_09856</i> gene through Agrobacterium-mediated transformation. We then analyzed the biological characteristics of the mutant strain and the virulence of the mutant strain toward <i>Ostrinia furnacalis</i> larvae, as well as its antagonistic effects against the phytopathogen <i>Botrytis cinerea</i>. We found that the average growth rate of the three mutant strains, ∆<i>BBA09856</i>-WT, was significantly higher compared to the wild-type (WT) strain on the 15th day of culture on potato dextrose agar (PDA) plates (7.01 cm vs. 6.30 cm, <i>p</i> < 0.01). Additionally, the average spore production(3.16 × 10⁷/cm² vs. 9.95 × 10⁶/cm², <i>p</i> < 0.001) and germination rate (82.50% vs. 54.72%, 12 h, <i>p</i> < 0.001) were significantly different between the three mutant strains, ∆<i>BBA09856</i>-WT, and the WT strain. The average survival rates of <i>O. furnacalis</i> infected with the WT strain and the three mutant strains, ∆<i>BBA09856</i>-WT, after 8 days were 61.66%, and 30.00%, respectively, indicating that the pathogenicity of the tested mutant strains was significantly greater than that of the WT strain. The results of the dual culture test indicated that the inhibitory rates of the WT and ∆<i>BBA09856</i>-WT strains against <i>B. cinerea</i> were 40.25% and 47.65%, respectively (<i>p</i> < 0.001). Similarly, in the dual culture test, the WT strain reduced the growth of <i>B. cinerea</i> by 9.90%, while the ∆<i>BBA09856</i>-WT exhibited a significantly greater inhibition rate of 28.29% (<i>p</i> < 0.05). The diameters of disease spots, measured 6 d after inoculation with <i>B. cinerea</i> in the tomato treatment groups, revealed significant differences in endophytic colonization between the WT and ∆<i>BBA09856</i>-WT strains in the WT+Bc and ∆<i>BBA09856</i>-WT+Bc treatment groups (15.26 mm vs. 12.16 mm, <i>p</i> < 0.01). Notably, ∆<i>BBA09856</i>-WT exhibited enhanced virulence toward <i>O. furnacalis</i> larvae and increased antagonistic activity against <i>B. cinerea</i>. Our results indicate that the gene <i>BBA_09856</i> may have a negative correlation with the development and virulence of <i>B. bassiana</i> toward the insect pest <i>O. furnacalis</i> larvae, as well as its antagonism against <i>B. cinerea</i>. These findings suggest that molecular techniques, such as gene editing, could be employed to develop superior strains of <i>B. bassiana</i> for the biological control of plant diseases and insect pests.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lichens and Health-Trends and Perspectives for the Study of Biodiversity in the Antarctic Ecosystem.","authors":"Tatiana Prado, Wim Maurits Sylvain Degrave, Gabriela Frois Duarte","doi":"10.3390/jof11030198","DOIUrl":"10.3390/jof11030198","url":null,"abstract":"<p><p>Lichens are an important vegetative component of the Antarctic terrestrial ecosystem and present a wide diversity. Recent advances in omics technologies have allowed for the identification of lichen microbiomes and the complex symbiotic relationships that contribute to their survival mechanisms under extreme conditions. The preservation of biodiversity and genetic resources is fundamental for the balance of ecosystems and for human and animal health. In order to assess the current knowledge on Antarctic lichens, we carried out a systematic review of the international applied research published between January 2019 and February 2024, using the PRISMA model (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). Articles that included the descriptors \"lichen\" and \"Antarctic\" were gathered from the web, and a total of 110 and 614 publications were retrieved from PubMed and ScienceDirect, respectively. From those, 109 publications were selected and grouped according to their main research characteristics, namely, (i) biodiversity, ecology and conservation; (ii) biomonitoring and environmental health; (iii) biotechnology and metabolism; (iv) climate change; (v) evolution and taxonomy; (vi) reviews; and (vii) symbiosis. Several topics were related to the discovery of secondary metabolites with potential for treating neurodegenerative, cancer and metabolic diseases, besides compounds with antimicrobial activity. Survival mechanisms under extreme environmental conditions were also addressed in many studies, as well as research that explored the lichen-associated microbiome, its biodiversity, and its use in biomonitoring and climate change, and reviews. The main findings of these studies are discussed, as well as common themes and perspectives.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942898/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patho-Ecological Distribution and Genetic Diversity of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> in Malbhog Banana Belts of Assam, India.","authors":"Anisha Baruah, Popy Bora, Thukkaram Damodaran, Bishal Saikia, Muthukumar Manoharan, Prakash Patil, Ashok Bhattacharyya, Ankita Saikia, Alok Kumar, Sangeeta Kumari, Juri Talukdar, Utpal Dey, Shenaz Sultana Ahmed, Naseema Rahman, Bharat Chandra Nath, Ruthy Tabing, Sandeep Kumar","doi":"10.3390/jof11030195","DOIUrl":"10.3390/jof11030195","url":null,"abstract":"<p><p>Fusarium wilt, caused by <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (Foc), is recognized as one of the most devastating diseases affecting banana cultivation worldwide. In India, Foc extensively affects Malbhog banana (AAB genomic group) production. In this study, we isolated 25 Foc isolates from wilt-affected Malbhog plantations inIndia. A pathogenicity test confirmed the identity of these isolates as Foc, the primary causative agent of wilt in bananas. The morpho-cultural characterization of Foc isolates showed large variations in colony morphological features, intensity, and pattern of pigmentation, chlamydospores, and conidial size. The molecular identification of these isolates using Race1- and Race4-specific primers established their identity as Race1 of Foc, with the absence of Tropical Race 4 of Foc. For a more comprehensive understanding of the genetic diversity of Foc isolates, we employed ISSR molecular typing, which revealed five major clusters. About 96% of the diversity within the Foc population indicated the presence of polymorphic loci in individuals of a given population evident from the results of Nei's genetic diversity, Shannon's information index, and the polymorphism information content values, apart from the analysis of molecular variance (AMOVA). The current findings provide significant insights toward the detection of Foc variants and, consequently, the deployment of effective management practices to keep the possible epidemic development of disease under control along the Malbhog banana growing belts of northeast India.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Insights into Cobweb Disease Resistance in <i>Agaricus bisporus</i>: A Comparative Analysis of Resistant and Susceptible Strains.","authors":"Guohui Cheng, Xiaoya An, Yueting Dai, Changtian Li, Yu Li","doi":"10.3390/jof11030200","DOIUrl":"10.3390/jof11030200","url":null,"abstract":"<p><p><i>Agaricus bisporus</i>, a globally cultivated edible fungus, faces significant challenges from fungal diseases like cobweb disease caused by <i>Cladobotryum mycophilum</i>, which severely impacts yield. This study aimed to explore the genetic basis of disease resistance in <i>A. bisporus</i> by comparing the genomes of a susceptible strain (AB7) and a resistant strain (AB58). Whole-genome sequencing of AB7 was performed using PacBio Sequel SMRT technology, and comparative genomic analyses were conducted alongside AB58 and other fungal hosts of <i>C. mycophilum</i>. Comparative genomic analyses revealed distinct resistance features in AB58, including enriched regulatory elements, specific deletions in AB7 affecting carbohydrate-active enzymes (CAZymes), and unique cytochrome P450 (CYP) profiles. Notably, AB58 harbored more cytochrome P450 genes related to fatty acid metabolism and unique NI-siderophore synthetase genes, contributing to its enhanced environmental adaptability and disease resistance. Pan-genome analysis highlighted significant genetic diversity, with strain-specific genes enriched in pathways like aflatoxin biosynthesis and ether lipid metabolism, suggesting distinct evolutionary adaptations. These findings provide valuable insights into the genetic basis underlying disease resistance in <i>A. bisporus</i>, offering a foundation for future breeding strategies to improve fungal crop resilience.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut Mycobiome Changes During COVID-19 Disease.","authors":"Danil V Krivonos, Dmitry E Fedorov, Ksenia M Klimina, Vladimir A Veselovsky, Svetlana N Kovalchuk, Alexander V Pavlenko, Oleg O Yanushevich, Dmitry N Andreev, Filipp S Sokolov, Aleksey K Fomenko, Mikhail K Devkota, Nikolai G Andreev, Andrey V Zaborovsky, Sergei V Tsaregorodtsev, Vladimir V Evdokimov, Natella I Krikheli, Petr A Bely, Oleg V Levchenko, Igor V Maev, Vadim M Govorun, Elena N Ilina","doi":"10.3390/jof11030194","DOIUrl":"10.3390/jof11030194","url":null,"abstract":"<p><p>The majority of metagenomic studies are based on the study of bacterial biota. At the same time, the COVID-19 pandemic has prompted interest in the study of both individual fungal pathogens and fungal communities (i.e., the mycobiome) as a whole. Here, in this work, we investigated the human gut mycobiome during COVID-19. Stool samples were collected from patients at two time points: at the time of admission to the hospital (the first time point) and at the time of discharge from the hospital (the second time point). The results of this study revealed that <i>Geotrichum</i> sp. is more represented in a group of patients with COVID-19. Therefore, <i>Geotrichum</i> sp. is elevated in patients at the time of admission to the hospital and underestimated at the time of discharge. Additionally, the influence of factors associated with the diversity of fungal gut microbiota was separately studied, including disease severity and age factors.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is the Co-Occurrence of <i>Neophysopella meliosmae-myrianthae</i> and <i>N. montana</i> (<i>Pucciniales</i>) Common on Grapevines in Japan?","authors":"Izumi Okane, Akiko Kurita, Yoshitaka Ono","doi":"10.3390/jof11030193","DOIUrl":"10.3390/jof11030193","url":null,"abstract":"<p><p>Grapevine leaf rust (GLR) in temperate Asia is caused by <i>Neophysopella meliosmae-myrianthae</i> and <i>N. montana</i>; the former is commonly found on commercial grape cultivars (<i>Vitis</i> spp.) and the latter on a wild grape species, <i>Vitis coignetiae</i>. The two GLR fungi were found to co-occur in <i>V. coignetiae</i> at two survey sites in Japan. Under experimental conditions, both fungi parasitize and develop into uredinial and telial stages on commercial grape cultivars and wild species. Despite the assumed involvement of <i>N. montana</i> in GLR symptoms in commercial vineyards, there has been no confirmed report of its incidence, and it is not clear whether <i>N. meliosmae-myrianthae</i> commonly occurs on <i>V. coignetiae</i> under natural conditions. In this study, we aimed to disclose the occurrence and, in particular, the co-occurrence of the two species in a wide array of commercial grape cultivars and <i>V. coignetiae</i> in Japan based on the detection of targeted DNA markers with specific PCR primer pairs. This study confirmed the occurrence of only <i>N. meliosmae-myrianthae</i> infection in symptomatic samples of grape cultivars, while the co-occurrence was observed only in <i>V. coignetiae</i>. <i>Neophysopella montana</i> was widely detected in <i>V. coignetiae</i> specimens.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Preliminary Exploration of Transcriptome and Proteomic Changes During the Young and Harvest Periods in <i>Morchella sextelata</i>.","authors":"Weilin Feng, Zier Guo, Qunli Jin, Fei Xu, Yingyue Shen, Tingting Song, Mei Wang, Jun Zhang, Lijun Fan, Xianbin Huang, Weiming Cai","doi":"10.3390/jof11030192","DOIUrl":"10.3390/jof11030192","url":null,"abstract":"<p><p>Based on transcriptome and proteome sequencing technologies, this study aims to preliminarily reveal the molecular mechanisms of growth and development and related metabolic regulation in <i>Morchella sextelat</i>. A total of 42.31 GB of Clean Data was acquired from the transcriptome sequencing (RNA-seq) of six samples in two development phases (<i>n</i> = 3) of <i>M. sextelata.</i> In the young phase (YP) and harvest phase (HP), there were 2887 differentially expressed genes (DEGs), including 1910 up-regulated genes and 977 down-regulated genes. In YP and HP, there were 987 differentially expressed proteins (DEPs), including 417 up-regulated ones and 570 down-regulated ones. Based on GO and KEGG analysis, significant differences in the transcriptomes and proteins in metabolic pathways are disclosed. Glycometabolism, especially starch, saccharose, and polysaccharide metabolism, plays a crucial role in the growth of <i>M. sextelata.</i> In addition, expression changes in the genes related to selenium metabolism are here recognized. These research results not only offer strong support for further exploration of the biological significance and functional differences of <i>M. sextelata</i>, but are also conducive to discovering key genes and understanding their regulation network during growth.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943006/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Oxalic Acid Consumption and pH on the In Vitro Biological Control of Oxalogenic Phytopathogen <i>Sclerotinia sclerotiorum</i>.","authors":"Aislinn Estoppey, Armelle Vallat-Michel, Patrick S Chain, Saskia Bindschedler, Pilar Junier","doi":"10.3390/jof11030191","DOIUrl":"10.3390/jof11030191","url":null,"abstract":"<p><p>The phytopathogenic fungus <i>Sclerotinia sclerotiorum</i> has a wide host range and causes significant economic losses in crops worldwide. This pathogen uses oxalic acid as a virulence factor; for this reason, the degradation of this organic acid by oxalotrophic bacteria has been proposed as a biological control approach. However, previous studies on the potential role of oxalotrophy in biocontrol did not investigate the differential effect of oxalic acid consumption and the subsequent pH alkalinisation on fungal growth. In this study, confrontation experiments on different media using a wild-type (WT) strain of <i>S. sclerotiorum</i> and an oxalate-deficient mutant (strain Δ<i>oah</i>) with the soil oxalotrophic bacteria <i>Cupriavidus necator</i> and <i>Cupriavidus oxalaticus</i> showed the combined effect of media composition on oxalic acid production, pH, and fungal growth control. Oxalotrophic bacteria were able to control <i>S. sclerotiorum</i> only in the medium in which oxalic acid was produced. However, the deficient Δ<i>oah</i> mutant was also controlled, indicating that the consumption of oxalic acid is not the sole mechanism of biocontrol. WT <i>S. sclerotiorum</i> acidified the medium when inoculated alone, while for both fungi, the pH of the medium changed from neutral to alkaline in the presence of bacteria. Therefore, medium alkalinisation independent of oxalotrophy contributes to fungal growth control.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Purification and Characterization of a Novel Neutral Protease from <i>Volvariella volvacea</i> Fruiting Bodies and the Enzymatic Digestion of Soybean Isolates.","authors":"Baoting Xu, Zhiping Li, Qian Guo, Lei Zha, Chuanhua Li, Panling Yu, Mingjie Chen, Yan Zhao","doi":"10.3390/jof11030190","DOIUrl":"10.3390/jof11030190","url":null,"abstract":"<p><p>A novel protease was isolated from the fruiting bodies of the straw mushroom <i>Volvariella volvacea</i>. The protease was purified 13.48-fold using a series of techniques, including ammonium sulfate precipitation, ultrafiltration, diethylaminoethyl fast-flow (DEAE FF) ion-exchange chromatography, and Superdex 75 gel filtration chromatography, resulting in a specific enzyme activity of 286.82 U/mg toward casein as a substrate. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that the purified protease had a molecular weight of 24 kDa. The enzyme exhibited optimal activity at pH 7 and 50 °C, showing sensitivity to alkaline conditions and instability at elevated temperatures. The presence of Ca²⁺ significantly enhanced enzyme activity, whereas Ni²⁺ and Cu²⁺ exerted strong inhibitory effects, with other metal ions showing weak inhibition. β-mercaptoethanol, Tween-80, and Triton X-100 had more pronounced inhibitory effects, whereas PMSF, EDTA, and CTAB had weaker inhibitory effects. The Michaelis constant (Km) and maximum velocity (Vm) of the protease were determined to be 1.34 g/L and 3.45 μg/(mL·min), respectively. The protease exhibited a greater degree of enzymatic degradation of soybean-isolate protein (7.58%) compared to trypsin (5.24%), with the enzyme product containing a high percentage of medicinal amino acids (73.54%), particularly phenylalanine (Phe) and arginine (Arg), suggesting their presence at the enzyme's active site. These findings suggest that the protease from <i>V. volvacea</i> holds promising potential for applications in the food industry, particularly in protein hydrolysate production and flavor enhancement.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Twist in Yeast: New Perspectives for Studying TDP-43 Proteinopathies in <i>S. cerevisiae</i>.","authors":"Roberto Stella, Alessandro Bertoli, Raffaele Lopreiato, Caterina Peggion","doi":"10.3390/jof11030188","DOIUrl":"10.3390/jof11030188","url":null,"abstract":"<p><p>TAR DNA-binding protein 43 kDa (TDP-43) proteinopathies are a group of neurodegenerative diseases (NDs) characterized by the abnormal accumulation of the TDP-43 protein in neurons and glial cells. These proteinopathies are associated with several NDs, including amyotrophic lateral sclerosis, frontotemporal lobar degeneration, and some forms of Alzheimer's disease. Yeast models have proven valuable in ND research due to their simplicity, genetic tractability, and the conservation of many cellular processes shared with higher eukaryotes. For several decades, <i>Saccharomyces cerevisiae</i> has been used as a model organism to study the behavior and toxicity of TDP-43, facilitating the identification of genes and pathways that either exacerbate or mitigate its toxic effects. This review will discuss evidence showing that yeast models of TDP-43 exhibit defects in proteostasis, mitochondrial function, autophagy, and RNA metabolism, which are key features of TDP-43-related NDs. Additionally, we will explore how modulating proteins involved in these processes reduce TDP-43 toxicity, aiding in restoring normal TDP-43 function or preventing its pathological aggregation. These findings highlight potential therapeutic targets for the treatment of TDP-43-related diseases.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 3","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143710196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}