Journal of Fungi最新文献

{"title":"The First Whole Genome Sequence and Methylation Profile of <i>Gerronema lapidescens</i> QL01.","authors":"Yanming Qiao, Zhiyuan Jia, Yuying Liu, Na Zhang, Chun Luo, Lina Meng, Yajie Cheng, Minglei Li, Xiuchao Xie, Jianzhao Qi","doi":"10.3390/jof11090647","DOIUrl":"10.3390/jof11090647","url":null,"abstract":"<p><p><i>Gerronema lapidescens</i> (Lei Wan), a valued medicinal basidiomycete traditionally employed for antiparasitic and digestive ailments, faces severe conservation threats due to unsustainable wild harvesting and the absence of reliable cultivation protocols. To address this crisis and unlock its pharmacotherapeutic potential, we present the first chromosome-scale genome assembly and comprehensive methylome profile for the wild strain <i>G. lapidescens</i> QL01, domesticated from the Qinling Mountains. A multi-platform sequencing strategy (Illumina and PacBio HiFi) yielded a high-quality 82.23 Mb assembly anchored to 11 chromosomes, exhibiting high completeness (98.4% BUSCO) and 46.03% GC content. Annotation predicted 15,847 protein-coding genes, with 81.12% functionally assigned. Genome-wide analysis identified 8.46 million high-confidence single-nucleotide polymorphisms (SNPs). Notably, methylation profiling revealed 3.25 million methylation events, with elevated densities on chromosomes 4, 9, and 10, suggesting roles in gene silencing and environmental adaptation. Phylogenomic analyses clarified the evolutionary status of <i>G. lapidescens</i>, whilst gene family evolution indicated moderate dynamics reflecting niche adaptation. Carbohydrate-Active enzymes (CAZymes) analysis identified 521 enzymes, including 211 Glycoside Hydrolases (GHs), consistent with organic matter degradation. Additionally, 3279 SSRs were catalogued as molecular markers. This foundational resource elucidates <i>G. lapidescens</i>'s genetic architecture, epigenetic regulation, evolutionary history, and enzymatic toolkit, underpinning future research into medicinal compound biosynthesis, environmental adaptation, germplasm conservation, and sustainable cultivation.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal Susceptibilities of Rare Yeast Isolates.","authors":"Deniz Turan, Zafer Habip, Hakan Odabaşı, Esra Dömbekçi, Narin Gündoğuş, Merve Özmen, Sebahat Aksaray","doi":"10.3390/jof11090645","DOIUrl":"10.3390/jof11090645","url":null,"abstract":"<p><p>The recent increase in the number of rare yeasts isolated from clinical specimens is a cause for concern, requiring accurate identification of these yeasts and assessment of their antifungal susceptibility to guide treatment. In this regard, we identified 196 rare yeasts isolated from various clinical specimens, mostly urine and respiratory tract specimens of patients hospitalized in intensive care unit and wards, using MALDI-TOF MS, and assessed their susceptibility to amphotericin B, fluconazole, voriconazole, posaconazole, itraconazole, isavuconazole and anidulafungin using the EUCAST broth microdilution method. Among the rare yeast species we isolated, <i>Candida lusitaniae</i> (13.8%) was the most common, followed by <i>Magnusiomyces capitatus</i> (13.3%), <i>Candida fabianii</i> (12.2%), and <i>Trichosporon asahii</i> (11.7%). Antifungal susceptibility testing revealed high echinocandin MIC values against <i>Magnusiomyces</i> spp., <i>Trichosporon</i> spp., and <i>Rhodotorula mucilaginosa</i> isolates. Similarly, we found high MIC values for fluconazole against the isolates of <i>Magnusiomyces</i> spp., <i>T. asahii</i>, <i>R. mucilaginosa</i>, and several <i>Candida</i> spp., including <i>Candida guilliermondii</i>, <i>Candida pararugosa</i>, <i>Candida rugosa</i>, <i>Candida pelliculosa</i>, <i>Candida norvegensis</i>, and <i>Candida fabianii</i>. We found similar MIC values across phylogenetically closely related species. In conclusion, prompt identification of rare yeasts and assessment of their antifungal susceptibilities are essential for effective treatment of the infections they cause.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rare Mould Fungaemia at a Tertiary Academic Hospital in Athens, Greece: A 15-Year Survey and Literature Review.","authors":"Maria Siopi, Angeliki Alevra, Dimitrios Mitsopoulos, Spyros Pournaras, Joseph Meletiadis","doi":"10.3390/jof11090644","DOIUrl":"10.3390/jof11090644","url":null,"abstract":"<p><p>Invasive infections caused by rare moulds (RM) are increasingly reported and often exhibit resistance to antifungal agents. Their epidemiology varies regionally, yet data from Greece are scarce. To address this gap, we conducted a 15-year retrospective study of RM fungaemia at a tertiary academic hospital in Athens, Greece. All microbiologically confirmed cases in hospitalised patients between 2010 and 2024 were reviewed. Demographic and clinical data were retrieved from medical records. Incidence rates were calculated per 1000 admissions and 10,000 bed-days. Isolates were morphologically identified and, when available, molecularly characterised and tested for antifungal susceptibility according to EUCAST guidelines. Eight RM fungaemia episodes (0.8% of total fungaemias) were identified, with an incidence of 0.01/1000 admissions and 0.03/10,000 bed-days, without bacterial co-infections. Haematological malignancies (62%) were the most common underlying condition. <i>Fusarium</i> spp. were the predominant pathogens (6/8), followed by single cases due to <i>Lomentospora prolificans</i> and <i>Acremonium</i> spp. Amphotericin B showed the highest in vitro activity against <i>Fusarium</i> isolates (MIC 0.5-1 mg/L), followed by voriconazole (MICs 2-8 mg/L) whereas other azoles showed no in vitro activity (MICs ≥ 8 mg/L). Half of the infections were breakthrough, whereas in 3/8 cases, the diagnosis was established post-mortem (n = 2) or post-discharge. Among the five patients who received treatment, the crude mortality rate was 60%. This first epidemiological report on RM fungaemia in Greece highlights the predominance of <i>Fusarium</i> spp., the frequency of breakthrough infections, and the challenges in early diagnosis and management. Increased clinical awareness and regional surveillance are essential for optimising outcomes.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isavuconazole Therapy for Patients with Hematologic Diseases and Hematopoietic Cell Transplantation with and Without Breakthrough Invasive Fungal Infections.","authors":"Fabián Herrera, Diego Torres, Gustavo Mendez, Noelia Mañez, Rosana Jordán, Adriana Manzur, Myrna Cabral, Manuel Alderete, Natalia García Allende, José Benso, Claudia Salgueira, María Laura Pereyra, Hugo Peretti, Carla Niveyro, Maximiliano Castro, Federico Pollastrelli, Silvina García Rojas, Juan Dapás, Agustina Risso Patrón, Verónica Fernández, Rocío Gago, Javier Afeltra","doi":"10.3390/jof11090648","DOIUrl":"10.3390/jof11090648","url":null,"abstract":"<p><p>There are no data available on the effectiveness and safety of isavuconazole (ISA) for treating breakthrough invasive fungal infections (bIFIs). A retrospective and prospective cohort study was conducted between January 2020 and March 2025 in 13 centers in Argentina. Hematologic diseases (HD) and hematopoietic cell transplantation (HCT) patients who received ISA for IFI were included and followed for 12 weeks. Patients with proven and probable bIFIs and non-bIFIs were compared. One hundred and sixty-three patients were included. IFIs were classified as proven (13.5%), probable (26.9%) and possible (59.5%). Among 66 proven and probable IFIs, 53% were bIFIs, with aspergillosis and mucormycosis being the most common. Twenty-three (34.8%) patients had acute myelogenous leukemia, and 40.9% had received HCT. Forty-eight (72.7%) patients experienced neutropenia, with a median duration of 26 days (interquartile range [IQR] 16-44). Fluconazole and posaconazole were the most frequently received antifungal prophylaxis. ISA was prescribed as first-line therapy in 31 (46.9%) patients. The other 35 received ISA as a continuation therapy, mainly as a step-down therapy after liposomal amphotericin B. Four (6.1%) patients developed adverse effects, and one discontinued ISA. The 90-day overall clinical response between patients with bIFI vs. non-bIFI was 91.4% vs. 70.9% (<i>p</i> = 0.052). The 90-day overall and IFI-related mortality rates were, respectively, 11.4% vs. 32.3% (<i>p</i> = 0.068) and 5.7% vs. 9.7% (<i>p</i> = 0.659). The study data evidence ISA effectiveness and safety for the treatment of HD and HCT patients with and without bIFIs.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blue Light Receptor WC-2 Regulates Ganoderic Acid Biosynthesis in <i>Ganoderma lingzhi</i>.","authors":"Yan Xu, Xiong-Min Huang, Zi-Xu Wang, Ying-Jie Zhao, Dong-Mei Lv, Jun-Wei Xu","doi":"10.3390/jof11090646","DOIUrl":"10.3390/jof11090646","url":null,"abstract":"<p><p>Ganoderic acid (GA) is a key bioactive component with pharmacological properties that is found in <i>Ganoderma lingzhi</i>, a renowned medicinal mushroom. Currently, the regulatory mechanisms underlying GA biosynthesis in <i>G. lingzhi</i> remain to be further elucidated. In this study, blue light induction was found to significantly enhance the GA content in <i>G. lingzhi</i>. To explore the regulatory mechanism of GA biosynthesis in response to blue light, the blue light receptor WC-2 was identified, and its regulatory role was characterized. The deletion of <i>wc-2</i> resulted in a significant reduction in both GA content and the accumulation of intermediates compared to the wild-type control strain, largely due to the strong downregulation of key GA biosynthetic genes. Additionally, decreased asexual spore production and reduced expression of sporulation-specific genes were observed with the deletion of <i>wc-2</i>. The overexpression of <i>wc-2</i> led to greatly enhanced GA accumulation. Under blue light induction, the maximum contents of GA-Mk, GA-T, GA-S, and GA-Me were 2.27-, 2.51-, 2.49-, and 2.08-fold higher, respectively, compared to the control kept in darkness. These results demonstrate that the blue light receptor WC-2 functions as a positive regulator of GA biosynthesis in <i>G. lingzhi</i>, influencing the expression of genes involved in GA biosynthesis and asexual spore production, thereby advancing our understanding of the intricate regulatory network of GA biosynthesis.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rise and Fall of <i>Phytophthora infestans</i> Resistance to Non-Specific Fungicide in Experimental Populations.","authors":"Shao-Bin Fan, Meng Xie, Zu-Lei Xiang, Tong-Xin Xu, Wen-Jing Wang, Zong-Hua Wang, Hong-Li Hu, Li-Xia Chen, Li Tang, Jia-Sui Zhan, Li-Na Yang","doi":"10.3390/jof11090643","DOIUrl":"10.3390/jof11090643","url":null,"abstract":"<p><p><b>Background</b>: Fungicide resistance is one of the major factors threatening social and ecological sustainability. Many issues associated with the evolutionary processes and mechanisms of fungicide resistance in pathogens remain poorly understood, and better knowledge of these issues through experimentally observing the rise and fall of the resistance is critical for the development of effective management strategies to ensure food security and ecological health. <b>Methods</b>: An experimental evolution approach was used to continuously acclimate a series of <i>Phytophthora infestans</i> populations under different mancozeb conditions for 400 consecutive days. <b>Results</b>: We found that <i>P. infestans</i> developed mancozeb resistance after 200 days of acclimation. This resistance was associated with ABC transporters and endocytic proteins. Potentially due to fitness costs associated with aggressiveness, mancozeb resistance was reversible. And the pathogen exhibited comparable rates of resistance gain during acclimation and resistance loss during the reversal experiment. <b>Conclusions</b>: Our results suggest that this pathogen may also develop resistance to mancozeb. However, this type of resistance may not be persistent, implying the fungicides concerned could be reused in practice. These results provide new insights into the evolution of fungicide resistance and sustainable plant disease chemical management based on the fungicide dose chosen beyond that of potato blight, warranting further study on the resistance target gene.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological and Genomic Insights into <i>Fusarium acuminatum</i> Causing Needle Blight in <i>Pinus tabuliformis</i>.","authors":"Linin Song, Yuying Xu, Tianjin Liu, He Wang, Xinyue Wang, Changxiao Fu, Xiaoling Xie, Yakubu Saddeeq Abubakar, Abah Felix, Ruixian Yang, Xinhong Jing, Guodong Lu, Jiandong Bao, Wenyu Ye","doi":"10.3390/jof11090636","DOIUrl":"10.3390/jof11090636","url":null,"abstract":"<p><p>Chinese pine, <i>Pinus tabuliformis</i>, is one of the most important garden plants in northern China, and the planting of this species is of great significance for the improvement of the ecological environment. In this study, different fungi were isolated and purified from diseased <i>Pinus tabuliformis</i> samples collected in Xi'an city, Shaanxi Province. Of these fungal isolates, only one (isolate AP-3) was pathogenic to the healthy host plant. The pathogenic isolate was identified as <i>Fusarium acuminatum</i> by morphological characteristics and <i>ITS</i> and <i>TEF-1α</i> sequence analyses. The optimal growth conditions for this isolate were further analyzed as follows: Optimal temperature of 25 °C, pH of 11, soluble starch and sodium nitrate as the most preferred carbon and nitrogen sources, respectively. By combining Oxford Nanopore Technologies (ONT) long-read sequencing with Illumina short-read sequencing technologies, we obtained a 41.50 Mb genome assembly for AP-3, with 47.97% GC content and 3.04% repeats. This consisted of 14 contigs with an N50 of 4.64 Mb and a maximum length of 6.45 Mb. The BUSCO completeness of the genome assembly was 98.94% at the fungal level and 97.83% at the Ascomycota level. The genome assembly contained 13,408 protein-coding genes, including 421 carbohydrate-active enzymes (CAZys), 120 cytochrome P450 enzymes (CYPs), 3185 pathogen-host interaction (PHI) genes, and 694 candidate secreted proteins. To our knowledge, this is the first report of <i>F. acuminatum</i> causing needle blight of <i>P. tabuliformis</i>. This study not only uncovered the pathogen responsible for needle blight of <i>P. tabuliformis</i>, but also provided a systematic analysis of its biological characteristics. These findings provide an important theoretical basis for disease control in <i>P. tabuliformis</i> and pave the way for further research into the fungal pathogenicity mechanisms and management strategies.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population, Physiological, and Genetic Insights into Carbendazim Resistance in Populations of the Phytopathogenic Fungus <i>Microdochium nivale</i>.","authors":"Guzalia Sh Murzagulova, Olga A Gogoleva, Egor A Ryazanov, Karina A Shatravina, Natalia V Tendiuk, Ildar T Sakhabutdinov, Sergey N Ponomarev, Inna B Chastukhina, Olga N Makshakova, Mira L Ponomareva, Vladimir Y Gorshkov","doi":"10.3390/jof11090639","DOIUrl":"10.3390/jof11090639","url":null,"abstract":"<p><p>Fungicide treatment is one of the most common methods for controlling fungal plant diseases. However, many phytopathogenic fungi develop resistance to fungicides. Addressing this agriculturally important issue requires comprehensive investigations into fungicide resistance. Our study aims to assess the degree and prevalence of resistance to carbendazim-one of the most widely used fungicides-in populations of <i>Microdochium nivale</i>, the causal agent of the deleterious plant disease pink snow mold; to explore possible relationships between carbendazim resistance and physiological and genetic traits; and to gain insight into the molecular basis of carbendazim resistance in this species. We showed that carbendazim resistance is widespread in the analyzed <i>M. nivale</i> populations, and that the application of carbendazim increases the proportion of resistant strains. Nevertheless, carbendazim-resistant strains are present at high relative abundance in populations that have never been exposed to fungicides. Carbendazim resistance in <i>M. nivale</i> is strongly associated with sequence variations in the β-tubulin gene, resulting in amino acid sequence variability that leads to differential affinity for carbendazim. Additionally, we propose a metabarcoding-based approach employing a genetic marker linked to a specific phenotypic trait to assess the ratio of genotypes with contrasting properties within a particular fungal species in environmental communities.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting the Structural Effects of CUG Codon Translation on Uncharacterized Proteins in <i>Candida albicans</i>.","authors":"Michaela Čermáková, Olga Heidingsfeld","doi":"10.3390/jof11090638","DOIUrl":"10.3390/jof11090638","url":null,"abstract":"<p><p>In the standard genetic code, the CUG triplet is translated as leucine. The pathogenic yeast <i>Candida albicans</i> and other CTG-clade yeasts contain tRNA_CAG, which is recognized by both leucine- and serine-tRNA synthetases. The CUG codon in these yeasts is translated most often as serine, and only in 3-5% of cases as leucine. Therefore, CTG <i>Candida</i> species have unstable proteomes. The effect of serine-leucine exchange on the structure and function of proteins has only been experimentally examined for a few cases. In <i>C. albicans</i>, CUG codons occur even in genes deemed to be essential. This means that serine-leucine ambiguity either does not affect the structure and function of the respective proteins, or that the presence of these amino acids at specific positions is associated with meaningful alteration of the proteins' function. This study employed AlphaFold2 to evaluate the potential effects of serine-to-leucine exchange in 12 proteins encoded by essential genes lacking orthologs in other yeasts and human genomes. The low homology with known proteins allowed us to make only low-confidence predictions. The analyzed proteins could be grouped into subsets based on the structural outcomes. Structural changes were observed only in four proteins. The remaining eight proteins showed no significant differences between serine and leucine variants.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Fusarium suttonianum</i> Identified as the Causal Agent of Root Rot in <i>Plukenetia volubilis</i> in Peru.","authors":"Elvin Delgado-Mera, Angel David Hernández-Amasifuen, Ángel Tuesta-Casique, Julio Santiago Chumacero-Acosta, Gerry Antonio Cosme-Garate, Gladstone Alves da Silva, Fernando Marcelo Carvajal Vallejos, Ronan Xavier Corrêa, Mike Anderson Corazon-Guivin","doi":"10.3390/jof11090642","DOIUrl":"10.3390/jof11090642","url":null,"abstract":"<p><p>Sacha inchi (<i>Plukenetia volubilis</i>) is a high-value crop due to its high content of omega-3 fatty acids and its outstanding nutritional, pharmaceutical, and cosmetic properties. However, this species faces challenges from diseases, particularly root rot. In this study, we identified one of the causal agents of root rot in sacha inchi using morphological observations, molecular methods, and pathogenicity tests. The pathogen was isolated from root tissues showing symptoms of <i>Fusarium</i> infection, observed in a plot in the Picota province. Morphological identification, DNA sequencing, and phylogenetic analysis using the <i>ITS</i> and <i>TEF-1α</i> markers revealed that the isolate causing root rot was <i>Fusarium suttonianum</i> (FSSC 20). Analysis of the PQ636870 (<i>ITS</i>) and PQ639345 (<i>TEF-1α</i>) sequences in the NCBI database, together with phylogenetic analysis, revealed 99.58% and 99.51% similarity with the <i>ITS</i> and <i>TEF</i> sequences, respectively, corresponding to <i>F. suttonianum</i>. Pathogenicity tests confirmed that this species induced the same symptoms observed in the field, fulfilling Koch's postulates. This study represents the first report of <i>F. suttonianum</i> as a pathogen causing root rot in sacha inchi in Peru. This finding is critical for developing effective strategies for disease management and control, contributing to the sustainability and improvement of sacha inchi production in the region.</p>","PeriodicalId":15878,"journal":{"name":"Journal of Fungi","volume":"11 9","pages":""},"PeriodicalIF":4.2,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}