Patho-Ecological Distribution and Genetic Diversity of Fusarium oxysporum f. sp. cubense in Malbhog Banana Belts of Assam, India.

Abstract

Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), is recognized as one of the most devastating diseases affecting banana cultivation worldwide. In India, Foc extensively affects Malbhog banana (AAB genomic group) production. In this study, we isolated 25 Foc isolates from wilt-affected Malbhog plantations inIndia. A pathogenicity test confirmed the identity of these isolates as Foc, the primary causative agent of wilt in bananas. The morpho-cultural characterization of Foc isolates showed large variations in colony morphological features, intensity, and pattern of pigmentation, chlamydospores, and conidial size. The molecular identification of these isolates using Race1- and Race4-specific primers established their identity as Race1 of Foc, with the absence of Tropical Race 4 of Foc. For a more comprehensive understanding of the genetic diversity of Foc isolates, we employed ISSR molecular typing, which revealed five major clusters. About 96% of the diversity within the Foc population indicated the presence of polymorphic loci in individuals of a given population evident from the results of Nei's genetic diversity, Shannon's information index, and the polymorphism information content values, apart from the analysis of molecular variance (AMOVA). The current findings provide significant insights toward the detection of Foc variants and, consequently, the deployment of effective management practices to keep the possible epidemic development of disease under control along the Malbhog banana growing belts of northeast India.