A.C. Valim , A.M.D. Costa , J.G. Quitzan , A.S. Borges , J.P. Oliveira-Filho , R.M. Amorim , D.G.A. Andrade
{"title":"Urothelial carcinoma in a mule","authors":"A.C. Valim , A.M.D. Costa , J.G. Quitzan , A.S. Borges , J.P. Oliveira-Filho , R.M. Amorim , D.G.A. Andrade","doi":"10.1016/j.jevs.2024.105127","DOIUrl":"https://doi.org/10.1016/j.jevs.2024.105127","url":null,"abstract":"<div><p>Equine bladder neoplasms are rare. This report aimed to describe the clinical signs and treatment of urothelial carcinoma (UC) in a mule. Cystoscopy of a 20-year-old female mule with a one-week history of hematuria and anemia revealed vascular congestion in the mucosa and an intraluminal, pedunculated mass in the dorsal bladder region. Histopathological examination revealed UC. Initial therapy consisted of four weekly cystoscopic guided injections of fluorouracil. At the fourth chemotherapy session, a paler and more friable tumor mass was observed. Consequently, we opted to surgically remove it during cystoscopy. Following mass excision, patient comfort, gross appearance of urine, and the hematocrit returned to normal. Repeat cystoscopy examinations revealed no gross appearance of tumor recurrence 18 months after treatment. Bladder neoplasms clinically resemble urolithiasis and cystitis and should be considered a differential diagnosis in cases of anemia and hematuria.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"139 ","pages":"Article 105127"},"PeriodicalIF":1.3,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141294705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pectin-honey hydrogel to prevent laparotomy surgical site infection in horses: A pilot study","authors":"Marco Gandini, Anna Cerullo, Gessica Giusto","doi":"10.1016/j.jevs.2024.105128","DOIUrl":"10.1016/j.jevs.2024.105128","url":null,"abstract":"<div><p>Surgical site infection (SSI) is a common complication after celiotomy in horses, leading to increased morbidity and costs. Increased concern about antibiotic resistance justifies evaluation of alternative preventive approaches, such a Manuka honey which has displayed antimicrobial properties. Pectin-Honey Hydrogels (PHH), composed by Manuka honey and pectin provide a moist wound environment and microbial growth inhibition. The aim of the study was to evaluate the effectiveness of PHHs in preventing SSI in horses subjected to emergency laparotomy. Horses undergoing laparotomy were evaluated. Horses were randomly divided into two groups: Group 1 received PHH application onto the sutured <em>linea alba</em> before skin closure, while Group 2 received no treatment. Horses with postoperative antimicrobial administration or survival of less than 5 days were excluded. The incidence of SSIs was reported as percentages and compared between groups. Out of 44 horses enrolled in the study, only thirty-six were ultimately included. Exclusions occurred either due to death before 5 days postoperatively (2 horses) or the administration of postoperative antimicrobials (6 horses). The median length of hospitalization was 9 days (range 8–14 days). The overall occurrence of SSI was 19.4 %. One out of eighteen horses (5.5 %) in Group 1 and 6 out of 18 (33.3 %) horses in Group 2 developed SSI. Group 2 had an 8.5-fold increased risk of SSI (<em>p</em> = 0.035, OR = 8.5, 95 % CI. 0.9–80.07). No macroscopically visible adverse reactions were associated with PHH. PHH placed at the abdominal incision during surgery was safe and reduced the prevalence of SSI in horses.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"139 ","pages":"Article 105128"},"PeriodicalIF":1.3,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Limited association between stallion-like behavior and hormonal indicators of testicular remnants in geldings","authors":"K. Omyla , A. Conley , D. Varner , P. Dini","doi":"10.1016/j.jevs.2024.105104","DOIUrl":"10.1016/j.jevs.2024.105104","url":null,"abstract":"<div><p>Persistent stallion-like behavior is a common sign of cryptorchidism in supposed geldings. The presence of testicular tissue can be evaluated by analyzing hormones such as testosterone and anti-Müllerian hormone (AMH). Here, we used hormonal analysis to investigate relationships between the likely presence of testicular tissue and stallion-like behavior in samples submitted from presumptive geldings (<em>n</em> = 1,202), retrospectively. Most geldings with stallion-like behaviors had serum concentrations of testosterone (851/1,056; 80.6 %) and AMH (682/877; 77.8 %) below the laboratory reference range for cryptorchids (< 60 pg/mL and ≤ 0.15 ng/mL for testosterone and AMH, respectively). A total of 13 samples (13/716; 1.8 %) showed AMH concentrations typical for geldings but testosterone above the cryptorchid range. Conversely, 31 samples (31/716; 4.3 %) had high AMH, suggesting cryptorchidism, but testosterone concentrations implied no testicular tissue. Among the cryptorchid stallions, the AMH and testosterone concentrations did not vary based on the season. However, age categories affected the concentration of both hormones among the presumptive true cryptorchid stallions. The results of this study demonstrate that undesirable behavior in geldings is rarely associated with the presence of testicular tissue, as assessed by these two hormonal biomarkers. This information highlights the complexity of behavior and demonstrates that persistent stallion-like behavior in geldings could be related to factors other than the presence of testicular tissue.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"139 ","pages":"Article 105104"},"PeriodicalIF":1.3,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0737080624001102/pdfft?md5=566c2c442dd0a19a997fcd5d3434d831&pid=1-s2.0-S0737080624001102-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dry matter concentration, particle size distribution and sand presence in faeces from horses with and without colic","authors":"Cecilia E. Müller","doi":"10.1016/j.jevs.2024.105126","DOIUrl":"10.1016/j.jevs.2024.105126","url":null,"abstract":"<div><p>A study comprising 74 colic and 74 control horses admitted to an animal hospital was performed. Faecal samples were collected and analysed for dry matter concentration, particle size distribution using wet-sieving, and sand presence through a sand sedimentation test. Data on horse breed, age, gender and basic feeding variables was collected and analysed using χ<sup>2</sup>-tests. Faecal dry matter concentration, particle size distribution and sand score was compared between colic and non-colic horses, and between horses with different colic types, using one-way ANOVA. Results showed that colic and non-colic horse groups were similar in breed, age, gender and basic feeding variables. Faecal dry matter concentration, particle size distribution and sand score were similar among colic and non-colic horses. Horses diagnosed with “unknown colic cause” had higher proportion of particles >0.5 <1.0 mm size compared to horses with colic due to impactions in caecum or colon, torsion or gas accumulation (<em>P</em><0.05), but this difference was very small and most likely not of biological importance. Faecal dry matter concentration and sand score were similar among horses with different types of colic. Increased knowledge of the composition of particles of different size in equine faeces may enhance our understanding of digesta passage rate in colic and non-colic horses, which is needed to develop preventative measures of certain types of colic.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"139 ","pages":"Article 105126"},"PeriodicalIF":1.3,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0737080624001321/pdfft?md5=74821b78b10152caca5d32c25d5cc231&pid=1-s2.0-S0737080624001321-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A.H. Silva , J.E.C. Lucena , F.M.P. Taran , D.S. Cunha , J.A.T. Almeida , R.S. Lima , W.S. Dias , J.M. Santiago
{"title":"Tropical grass digestibility assessed by the mobile bag and in vitro methods in horses","authors":"A.H. Silva , J.E.C. Lucena , F.M.P. Taran , D.S. Cunha , J.A.T. Almeida , R.S. Lima , W.S. Dias , J.M. Santiago","doi":"10.1016/j.jevs.2024.105112","DOIUrl":"10.1016/j.jevs.2024.105112","url":null,"abstract":"<div><p>This study aimed to compare the digestibility of tropical grasses by horses by the <em>in vivo</em> method using mobile nylon bags with the <em>in vitro</em> digestibility method using horse feces as a source of inoculum. Five horses were used in a 2 × 5 factorial design with randomized blocks featuring two methods (<em>in vivo</em> and <em>in vitro</em>) and five grasses: Tifton 85 hay (<em>Cynodon</em> spp.), sixweeks threeawn grass (<em>Aristida adsencionis,</em> Linn), Alexandergrass (<em>Brachiaria plantaginea</em> (Link) Hitchc.), capim-de-raiz (<em>Chloris orthonoton</em>, Doell), and Sabi grass (<em>Urochloa mosambicensis</em>). No difference (<em>P>0.05</em>) was found between the <em>in vivo</em> and in <em>vitro</em> methods regarding nutrient digestibility of Sabi grass and sixweeks threeawn. Tifton 85 was the only grass that showed differences (<em>P<0.05</em>) between the two methods concerning the apparent digestibility of all nutrients. Alexandergrass, Tifton 85, and capim-de-raiz exhibited the best digestibility of dry matter, neutral detergent fiber, acid detergent fiber, and organic matter by the mobile bag method compared to the <em>in vitro</em> method. Tifton 85 and capim-de-raiz had higher crude protein digestibility by the mobile bag method than by the <em>in vitro</em> method. The mean retention time of the mobile bags in the digestive tract of the horses was 43.69 h. The bags with samples of sixweeks threeawn and Sabi grass had shorter retention times than capim-de-raiz and Alexandergrass (<em>P<0.0001</em>). It is concluded that, for sixweeks threeawn and Sabi grass, digestibility in horses can be assessed using the <em>in vitro</em> method in place of the mobile nylon bag method.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"139 ","pages":"Article 105112"},"PeriodicalIF":1.3,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141275384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Francesca Aragona , Francesca Arfuso , Maria Rizzo , Francesco Fazio , Giuseppe Acri , Giuseppe Piccione , Claudia Giannetto
{"title":"Using infrared thermography for the evaluation of road transport thermal homeostasis in athletic horse","authors":"Francesca Aragona , Francesca Arfuso , Maria Rizzo , Francesco Fazio , Giuseppe Acri , Giuseppe Piccione , Claudia Giannetto","doi":"10.1016/j.jevs.2024.105102","DOIUrl":"10.1016/j.jevs.2024.105102","url":null,"abstract":"<div><p>This study aimed to evaluate changes in body temperature in athletic horses during two different road transport distances. Six Italian Saddle horses were subjected to a 100 and 300 km transport during different times of day (am and pm). Rectal and cutaneous temperatures were recorded before (T0), immediately (following 5 min- T1) and 1 hour (T2) after transport by means of a rectal digital thermometer and a thermal infrared camera (FLIR T440) respectively, for the evaluation of left and right side of four body regions: jugular, shoulder, croup and inner thigh. There were no differences between left and right sides, inner thigh or rectal temperatures when comparing the transport distance, time points or time of day. At T0, jugular (P < 0.0001), shoulder (P < 0.01) and croup (P < 0.01) average temperatures were higher in the <em>pm</em> compared to those in the <em>am</em> in both journeys. At T1, jugular (P < 0.01) and croup (P < 0.01) temperatures were lower in the <em>pm</em> compared to <em>am</em> following the 300 km journey. Jugular temperature (P < 0.0001) was higher following the 300 km compared to the 100 km journey at each time point (T1 and T2) at both times of day (am and pm). Shoulder (P < 0.0001) and croup temperatures (P < 0.0001) were higher at T2 after the 300 km journey than at T2 after the 100 km journey). The current results suggested a difference between the two distances and the time of day appeared to have as great effect on ocular temperature as road transport distance</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"138 ","pages":"Article 105102"},"PeriodicalIF":1.3,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Aguila , P. Cabrera , ME. Arias , M. Silva , R. Felmer
{"title":"Effect of sperm treatment with lysolecithin on in vitro outcomes of equine intracytoplasmic sperm injection","authors":"L. Aguila , P. Cabrera , ME. Arias , M. Silva , R. Felmer","doi":"10.1016/j.jevs.2024.105095","DOIUrl":"10.1016/j.jevs.2024.105095","url":null,"abstract":"<div><p>Intracytoplasmic sperm injection (ICSI) in horses is currently employed for clinical and commercial uses, but the protocol could be optimized to improve its efficiency. We have hypothesized that destabilization of plasma and acrosomal membranes prior to injection would positively impact the developmental potential of equine zygotes generated by ICSI. This study evaluated effects of the sperm treatment with lysolecithin on plasma and acrosomal membranes and on oocyte activation ability, initially following heterologous ICSI on bovine oocytes and subsequently employing equine oocytes. The effects of the lysolecithin -treatment on the efficiency of conventional and piezo-assisted equine ICSI were evaluated. To do this, the equine sperm were treated with different concentrations of lysolecithin and the sperm plasma membrane, acrosome and DNA integrity were evaluated by flow cytometry. The results showed that a lysolecithin concentration of 0.08 % destabilized the membranes of all sperm and affected DNA integrity within the range described for the species (8–30 %). In addition, the heterologous ICSI assay showed that lysolecithin treatment was detrimental to the sperm's ability to activate the oocyte, therefore, chemical oocyte activation was used after equine ICSI after injection with lysolecithin -treated sperm. This group showed similar developmental rate to the control group with and without exogenous activation. In conclusion, lysolecithin pre-treatment is not necessary when using ICSI to produce equine embryos <em>in vitro</em>. The results from the current study provide additional insight regarding the factors impacting ICSI in horses.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"138 ","pages":"Article 105095"},"PeriodicalIF":1.3,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ömer Deniz , Gencay Ekinci , Ali Cesur Onmaz , Fatih Mehmet Derelli , Francesco Fazio , Francesca Aragona , René van den Hoven
{"title":"Monitoring of inflammatory blood biomarkers in foals with Rhodococcus Equi pneumonia during antimicrobial treatment","authors":"Ömer Deniz , Gencay Ekinci , Ali Cesur Onmaz , Fatih Mehmet Derelli , Francesco Fazio , Francesca Aragona , René van den Hoven","doi":"10.1016/j.jevs.2024.105103","DOIUrl":"10.1016/j.jevs.2024.105103","url":null,"abstract":"<div><p><em>Rhodococcus equi (R. equi)</em>, a gram-positive facultative intracellular pathogen, is a common cause of pneumonia in foals and represents a major cause of disease and death. The aim of the present study was to investigate the time-depended changes in White Blood Cells (WBC), basophils (Baso), neutrophils (Neu), lymphocytes (Lymf), monocytes (Mon), eosinophils (Eos), platelet (PLT) counts, fibrinogen (Fbg) concentration, interferon (IFN-α, IFN-γ) and interleukins (IL-2 and IL-10) in foals with clinical <em>R. equi</em> pneumonia. The main treatment was with azithromycin-rifampicin for 14 days. Blood was sampled prior to, 7 and 14 days after starting therapy. Treatment was associated with significantly decreased counts of WBC, (25.6 ± 6.7 and 14.2 ± 2,7 × 10<sup>3</sup>/ml), Neu (18.6 ±6.2 and 10.7 ± 3.1 × 10<sup>3</sup>/ml), Mon (1.5 ± 0.5 and 0.9 ± 0.2 × 10<sup>3</sup>/ml) and Fbg (539 ± 124 and 287 ± 26 g/dl) between day 0 and day 14. IL-2 and IL-10 concentrations were significantly increased (<em>P</em> = 0.028, <em>P</em> = 0.013, respectively) after treatment, whereas IFN-α and IFN-γ concentrations were not. The diagnostic potentials of INF-α, INF-γ, IL-2 and IL-10 <em>per se</em> seems not very high, however, the study suggests that the activity change of selected interleukins in the course of the disease may be associated with amelioration. We concluded that patterns of serum concentration changes of INF-α, INF-γ, IL-2 and IL-10 may help in the study of the innate immune response in foals during infection and treatment of <em>R. equi</em> pneumonia.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"138 ","pages":"Article 105103"},"PeriodicalIF":1.3,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141137555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Equine Talocalcaneal subluxation","authors":"Lily Bertelsen","doi":"10.1016/j.jevs.2024.105101","DOIUrl":"10.1016/j.jevs.2024.105101","url":null,"abstract":"<div><p>A Quarter horse filly was evaluated for a two-week old wound of the right hind cannon bone after the mare stepped on her. A sequestrum was noted radiographically and was surgically debrided. After continued follow-up a left hind varus deformity was noted and was corrected with a lateral transphyseal screw in the distal left tibia. After the varus deformity was improved, a lateromedial radiograph was taken of the distal left tibia and tarsus and talocalcaneal subluxation was seen. The same view was taken of the right tarsus and the same deformity was noted. After finding no case reports on the condition with information on treatment or surgical correction, humane euthanasia was elected. A few months later, the same owner brought a two-month-old colt out of the same mare to the hospital for an elective procedure. Tarsal radiographs were taken due to the owner's previous experience, although the colt had no clinical history or any traumatic incidents. A lateromedial view of the right tarsus revealed talocalcaneal subluxation.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"138 ","pages":"Article 105101"},"PeriodicalIF":1.3,"publicationDate":"2024-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141071116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}